LOCUS       DACDSR010000001       118925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDSR010000001 DACDSR010000000
VERSION     DACDSR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 118925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 118925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..118925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.001"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..291
                     /locus_tag="IWK15_000001"
     CDS             <1..291
                     /locus_tag="IWK15_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HAP9545634.1"
                     /translation="FKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSD
                     KGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            491..805
                     /locus_tag="IWK15_000002"
     CDS             491..805
                     /locus_tag="IWK15_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9545635.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            826..1203
                     /locus_tag="IWK15_000003"
     CDS             826..1203
                     /locus_tag="IWK15_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9545636.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1213..1986
                     /locus_tag="IWK15_000004"
     CDS             1213..1986
                     /locus_tag="IWK15_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545637.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2008..3411
                     /locus_tag="IWK15_000005"
     CDS             2008..3411
                     /locus_tag="IWK15_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9545638.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3593..4777
                     /locus_tag="IWK15_000006"
     CDS             3593..4777
                     /locus_tag="IWK15_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9545639.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4774..5064
                     /locus_tag="IWK15_000007"
     CDS             4774..5064
                     /locus_tag="IWK15_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545640.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5061..5282
                     /locus_tag="IWK15_000008"
     CDS             5061..5282
                     /locus_tag="IWK15_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545641.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5324..6103
                     /locus_tag="IWK15_000009"
     CDS             5324..6103
                     /locus_tag="IWK15_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9545642.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6170..6664
                     /locus_tag="IWK15_000010"
     CDS             6170..6664
                     /locus_tag="IWK15_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9545643.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6720..7358
                     /locus_tag="IWK15_000011"
     CDS             6720..7358
                     /locus_tag="IWK15_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545644.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7430..7825
                     /locus_tag="IWK15_000012"
     CDS             7430..7825
                     /locus_tag="IWK15_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9545645.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7809..10262
                     /locus_tag="IWK15_000013"
     CDS             7809..10262
                     /locus_tag="IWK15_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9545646.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10259..12286
                     /locus_tag="IWK15_000014"
     CDS             10259..12286
                     /locus_tag="IWK15_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545647.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12283..13305
                     /locus_tag="IWK15_000015"
     CDS             12283..13305
                     /locus_tag="IWK15_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9545648.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13322..14251
                     /locus_tag="IWK15_000016"
     CDS             13322..14251
                     /locus_tag="IWK15_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9545649.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14496..14612
                     /locus_tag="IWK15_000017"
     CDS             14496..14612
                     /locus_tag="IWK15_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9545650.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14628..15431
                     /locus_tag="IWK15_000018"
     CDS             14628..15431
                     /locus_tag="IWK15_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9545651.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15606..15902
                     /locus_tag="IWK15_000019"
     CDS             15606..15902
                     /locus_tag="IWK15_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545652.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15909..17861
                     /locus_tag="IWK15_000020"
     CDS             15909..17861
                     /locus_tag="IWK15_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9545653.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17933..18430)
                     /gene="dfrG"
                     /locus_tag="IWK15_000021"
     CDS             complement(17933..18430)
                     /gene="dfrG"
                     /locus_tag="IWK15_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9545654.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18792..19832
                     /locus_tag="IWK15_000022"
     CDS             18792..19832
                     /locus_tag="IWK15_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9545655.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            19930..20118
                     /locus_tag="IWK15_000023"
     CDS             19930..20118
                     /locus_tag="IWK15_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9545656.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20176..20529)
                     /locus_tag="IWK15_000024"
     CDS             complement(20176..20529)
                     /locus_tag="IWK15_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9545657.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21060..21482
                     /locus_tag="IWK15_000025"
     CDS             21060..21482
                     /locus_tag="IWK15_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9545658.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21479..21709
                     /locus_tag="IWK15_000026"
     CDS             21479..21709
                     /locus_tag="IWK15_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9545659.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22207..22407
                     /locus_tag="IWK15_000027"
     CDS             22207..22407
                     /locus_tag="IWK15_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9545660.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22434..23627
                     /locus_tag="IWK15_000028"
     CDS             22434..23627
                     /locus_tag="IWK15_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9545661.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23695..25260)
                     /gene="guaA"
                     /locus_tag="IWK15_000029"
     CDS             complement(23695..25260)
                     /gene="guaA"
                     /locus_tag="IWK15_000029"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9545662.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25319..26242)
                     /locus_tag="IWK15_000030"
     CDS             complement(25319..26242)
                     /locus_tag="IWK15_000030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9545663.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26438..27043
                     /locus_tag="IWK15_000031"
     CDS             26438..27043
                     /locus_tag="IWK15_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9545664.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27351..28367
                     /locus_tag="IWK15_000032"
     CDS             27351..28367
                     /locus_tag="IWK15_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9545665.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28508..29809
                     /locus_tag="IWK15_000033"
     CDS             28508..29809
                     /locus_tag="IWK15_000033"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9545666.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29855..30517
                     /gene="deoC"
                     /locus_tag="IWK15_000034"
     CDS             29855..30517
                     /gene="deoC"
                     /locus_tag="IWK15_000034"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9545667.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30538..30936
                     /locus_tag="IWK15_000035"
     CDS             30538..30936
                     /locus_tag="IWK15_000035"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9545668.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31002..32084
                     /locus_tag="IWK15_000036"
     CDS             31002..32084
                     /locus_tag="IWK15_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9545669.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32296..33858
                     /locus_tag="IWK15_000037"
     CDS             32296..33858
                     /locus_tag="IWK15_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545670.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33851..34984
                     /locus_tag="IWK15_000038"
     CDS             33851..34984
                     /locus_tag="IWK15_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9545671.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34984..35940
                     /locus_tag="IWK15_000039"
     CDS             34984..35940
                     /locus_tag="IWK15_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9545672.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36039..37205
                     /gene="deoB"
                     /locus_tag="IWK15_000040"
     CDS             36039..37205
                     /gene="deoB"
                     /locus_tag="IWK15_000040"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9545673.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37229..38047
                     /locus_tag="IWK15_000041"
     CDS             37229..38047
                     /locus_tag="IWK15_000041"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9545674.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38064..38771
                     /gene="deoD"
                     /locus_tag="IWK15_000042"
     CDS             38064..38771
                     /gene="deoD"
                     /locus_tag="IWK15_000042"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9545675.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39069..39755)
                     /gene="gpmA"
                     /locus_tag="IWK15_000043"
     CDS             complement(39069..39755)
                     /gene="gpmA"
                     /locus_tag="IWK15_000043"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9545676.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(39927..40508)
                     /locus_tag="IWK15_000044"
     CDS             complement(39927..40508)
                     /locus_tag="IWK15_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9545677.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40641..41324)
                     /gene="rpiA"
                     /locus_tag="IWK15_000045"
     CDS             complement(40641..41324)
                     /gene="rpiA"
                     /locus_tag="IWK15_000045"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9545678.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41643..42056
                     /gene="rpsL"
                     /locus_tag="IWK15_000046"
     CDS             41643..42056
                     /gene="rpsL"
                     /locus_tag="IWK15_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9545679.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42140..42610
                     /gene="rpsG"
                     /locus_tag="IWK15_000047"
     CDS             42140..42610
                     /gene="rpsG"
                     /locus_tag="IWK15_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9545680.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42704..44791
                     /gene="fusA"
                     /locus_tag="IWK15_000048"
     CDS             42704..44791
                     /gene="fusA"
                     /locus_tag="IWK15_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9545681.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            44963..46150
                     /gene="tuf"
                     /locus_tag="IWK15_000049"
     CDS             44963..46150
                     /gene="tuf"
                     /locus_tag="IWK15_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9545682.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46557..46865
                     /gene="rpsJ"
                     /locus_tag="IWK15_000050"
     CDS             46557..46865
                     /gene="rpsJ"
                     /locus_tag="IWK15_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9545683.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            46896..47525
                     /gene="rplC"
                     /locus_tag="IWK15_000051"
     CDS             46896..47525
                     /gene="rplC"
                     /locus_tag="IWK15_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9545684.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47553..48176
                     /gene="rplD"
                     /locus_tag="IWK15_000052"
     CDS             47553..48176
                     /gene="rplD"
                     /locus_tag="IWK15_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9545685.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48176..48466
                     /gene="rplW"
                     /locus_tag="IWK15_000053"
     CDS             48176..48466
                     /gene="rplW"
                     /locus_tag="IWK15_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9545686.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48505..49338
                     /gene="rplB"
                     /locus_tag="IWK15_000054"
     CDS             48505..49338
                     /gene="rplB"
                     /locus_tag="IWK15_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9545687.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49381..49659
                     /gene="rpsS"
                     /locus_tag="IWK15_000055"
     CDS             49381..49659
                     /gene="rpsS"
                     /locus_tag="IWK15_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9545688.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49682..50029
                     /gene="rplV"
                     /locus_tag="IWK15_000056"
     CDS             49682..50029
                     /gene="rplV"
                     /locus_tag="IWK15_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9545689.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50043..50699
                     /gene="rpsC"
                     /locus_tag="IWK15_000057"
     CDS             50043..50699
                     /gene="rpsC"
                     /locus_tag="IWK15_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9545690.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50702..51136
                     /gene="rplP"
                     /locus_tag="IWK15_000058"
     CDS             50702..51136
                     /gene="rplP"
                     /locus_tag="IWK15_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9545691.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51126..51314
                     /gene="rpmC"
                     /locus_tag="IWK15_000059"
     CDS             51126..51314
                     /gene="rpmC"
                     /locus_tag="IWK15_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9545692.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51339..51605
                     /gene="rpsQ"
                     /locus_tag="IWK15_000060"
     CDS             51339..51605
                     /gene="rpsQ"
                     /locus_tag="IWK15_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9545693.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51663..52031
                     /gene="rplN"
                     /locus_tag="IWK15_000061"
     CDS             51663..52031
                     /gene="rplN"
                     /locus_tag="IWK15_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9545694.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52068..52376
                     /gene="rplX"
                     /locus_tag="IWK15_000062"
     CDS             52068..52376
                     /gene="rplX"
                     /locus_tag="IWK15_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9545695.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52404..52943
                     /gene="rplE"
                     /locus_tag="IWK15_000063"
     CDS             52404..52943
                     /gene="rplE"
                     /locus_tag="IWK15_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9545696.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            52962..53147
                     /locus_tag="IWK15_000064"
     CDS             52962..53147
                     /locus_tag="IWK15_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9545697.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53183..53581
                     /gene="rpsH"
                     /locus_tag="IWK15_000065"
     CDS             53183..53581
                     /gene="rpsH"
                     /locus_tag="IWK15_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9545698.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53613..54149
                     /gene="rplF"
                     /locus_tag="IWK15_000066"
     CDS             53613..54149
                     /gene="rplF"
                     /locus_tag="IWK15_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9545699.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54310..54666
                     /gene="rplR"
                     /locus_tag="IWK15_000067"
     CDS             54310..54666
                     /gene="rplR"
                     /locus_tag="IWK15_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9545700.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54687..55187
                     /gene="rpsE"
                     /locus_tag="IWK15_000068"
     CDS             54687..55187
                     /gene="rpsE"
                     /locus_tag="IWK15_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9545701.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55202..55381
                     /gene="rpmD"
                     /locus_tag="IWK15_000069"
     CDS             55202..55381
                     /gene="rpmD"
                     /locus_tag="IWK15_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9545702.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55425..55865
                     /gene="rplO"
                     /locus_tag="IWK15_000070"
     CDS             55425..55865
                     /gene="rplO"
                     /locus_tag="IWK15_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9545703.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            55865..57160
                     /gene="secY"
                     /locus_tag="IWK15_000071"
     CDS             55865..57160
                     /gene="secY"
                     /locus_tag="IWK15_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9545704.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57222..57869
                     /locus_tag="IWK15_000072"
     CDS             57222..57869
                     /locus_tag="IWK15_000072"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9545705.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58060..58278
                     /gene="infA"
                     /locus_tag="IWK15_000073"
     CDS             58060..58278
                     /gene="infA"
                     /locus_tag="IWK15_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9545706.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58311..58427
                     /gene="rpmJ"
                     /locus_tag="IWK15_000074"
     CDS             58311..58427
                     /gene="rpmJ"
                     /locus_tag="IWK15_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9545707.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58445..58810
                     /gene="rpsM"
                     /locus_tag="IWK15_000075"
     CDS             58445..58810
                     /gene="rpsM"
                     /locus_tag="IWK15_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9545708.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            58838..59227
                     /gene="rpsK"
                     /locus_tag="IWK15_000076"
     CDS             58838..59227
                     /gene="rpsK"
                     /locus_tag="IWK15_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9545709.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59307..60245
                     /locus_tag="IWK15_000077"
     CDS             59307..60245
                     /locus_tag="IWK15_000077"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9545710.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60273..60653
                     /gene="rplQ"
                     /locus_tag="IWK15_000078"
     CDS             60273..60653
                     /gene="rplQ"
                     /locus_tag="IWK15_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9545711.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            60925..61764
                     /locus_tag="IWK15_000079"
     CDS             60925..61764
                     /locus_tag="IWK15_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9545712.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            61740..62609
                     /locus_tag="IWK15_000080"
     CDS             61740..62609
                     /locus_tag="IWK15_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9545713.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62599..63396
                     /locus_tag="IWK15_000081"
     CDS             62599..63396
                     /locus_tag="IWK15_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9545714.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63599..64345
                     /gene="truA"
                     /locus_tag="IWK15_000082"
     CDS             63599..64345
                     /gene="truA"
                     /locus_tag="IWK15_000082"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9545715.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64678..67347)
                     /locus_tag="IWK15_000083"
     CDS             complement(64678..67347)
                     /locus_tag="IWK15_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9545716.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67585..67773)
                     /locus_tag="IWK15_000084"
     CDS             complement(67585..67773)
                     /locus_tag="IWK15_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9545717.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            68043..68405
                     /locus_tag="IWK15_000085"
     CDS             68043..68405
                     /locus_tag="IWK15_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9545718.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68457..70139
                     /locus_tag="IWK15_000086"
     CDS             68457..70139
                     /locus_tag="IWK15_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9545719.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            70256..72292
                     /gene="recG"
                     /locus_tag="IWK15_000087"
     CDS             70256..72292
                     /gene="recG"
                     /locus_tag="IWK15_000087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9545720.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72341..73342
                     /gene="plsX"
                     /locus_tag="IWK15_000088"
     CDS             72341..73342
                     /gene="plsX"
                     /locus_tag="IWK15_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9545721.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73464..73706
                     /gene="acpP"
                     /locus_tag="IWK15_000089"
     CDS             73464..73706
                     /gene="acpP"
                     /locus_tag="IWK15_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9545722.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            73974..74978
                     /locus_tag="IWK15_000090"
     CDS             73974..74978
                     /locus_tag="IWK15_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545723.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            74979..75923
                     /locus_tag="IWK15_000091"
     CDS             74979..75923
                     /locus_tag="IWK15_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545724.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            75923..76885
                     /locus_tag="IWK15_000092"
     CDS             75923..76885
                     /locus_tag="IWK15_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9545725.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            76912..77826
                     /locus_tag="IWK15_000093"
     CDS             76912..77826
                     /locus_tag="IWK15_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9545726.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            77852..79633
                     /locus_tag="IWK15_000094"
     CDS             77852..79633
                     /locus_tag="IWK15_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9545727.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            79981..80667
                     /locus_tag="IWK15_000095"
     CDS             79981..80667
                     /locus_tag="IWK15_000095"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9545728.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            80687..84268
                     /gene="smc"
                     /locus_tag="IWK15_000096"
     CDS             80687..84268
                     /gene="smc"
                     /locus_tag="IWK15_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9545729.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            84277..85086
                     /locus_tag="IWK15_000097"
     CDS             84277..85086
                     /locus_tag="IWK15_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9545730.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            85097..86095
                     /gene="ftsY"
                     /locus_tag="IWK15_000098"
     CDS             85097..86095
                     /gene="ftsY"
                     /locus_tag="IWK15_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9545731.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            86282..88549
                     /gene="gshAB"
                     /locus_tag="IWK15_000099"
     CDS             86282..88549
                     /gene="gshAB"
                     /locus_tag="IWK15_000099"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9545732.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(88638..89090)
                     /locus_tag="IWK15_000100"
     CDS             complement(88638..89090)
                     /locus_tag="IWK15_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9545733.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            89240..90190
                     /locus_tag="IWK15_000101"
     CDS             89240..90190
                     /locus_tag="IWK15_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9545734.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            90183..91142
                     /locus_tag="IWK15_000102"
     CDS             90183..91142
                     /locus_tag="IWK15_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9545735.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            91139..91894
                     /locus_tag="IWK15_000103"
     CDS             91139..91894
                     /locus_tag="IWK15_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9545736.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            91917..92870
                     /locus_tag="IWK15_000104"
     CDS             91917..92870
                     /locus_tag="IWK15_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9545737.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(93514..93810)
                     /locus_tag="IWK15_000105"
     CDS             complement(93514..93810)
                     /locus_tag="IWK15_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9545738.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            94165..94521
                     /locus_tag="IWK15_000106"
     CDS             94165..94521
                     /locus_tag="IWK15_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9545739.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            94531..95430
                     /locus_tag="IWK15_000107"
     CDS             94531..95430
                     /locus_tag="IWK15_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9545740.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(95827..96906)
                     /locus_tag="IWK15_000108"
     CDS             complement(95827..96906)
                     /locus_tag="IWK15_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9545741.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            97142..97732
                     /locus_tag="IWK15_000109"
     CDS             97142..97732
                     /locus_tag="IWK15_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9545742.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(97860..99209)
                     /locus_tag="IWK15_000110"
     CDS             complement(97860..99209)
                     /locus_tag="IWK15_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9545743.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(99377..100117)
                     /locus_tag="IWK15_000111"
     CDS             complement(99377..100117)
                     /locus_tag="IWK15_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9545744.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(100130..101722)
                     /locus_tag="IWK15_000112"
     CDS             complement(100130..101722)
                     /locus_tag="IWK15_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9545745.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(102290..102586)
                     /locus_tag="IWK15_000113"
     CDS             complement(102290..102586)
                     /locus_tag="IWK15_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545746.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            102762..103487
                     /locus_tag="IWK15_000114"
     CDS             102762..103487
                     /locus_tag="IWK15_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9545747.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            103480..104322
                     /locus_tag="IWK15_000115"
     CDS             103480..104322
                     /locus_tag="IWK15_000115"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9545748.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            104325..104846
                     /locus_tag="IWK15_000116"
     CDS             104325..104846
                     /locus_tag="IWK15_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9545749.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(105099..105662)
                     /locus_tag="IWK15_000117"
     CDS             complement(105099..105662)
                     /locus_tag="IWK15_000117"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9545750.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            105913..106182
                     /gene="rpsO"
                     /locus_tag="IWK15_000118"
     CDS             105913..106182
                     /gene="rpsO"
                     /locus_tag="IWK15_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9545751.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            106370..108496
                     /gene="pnp"
                     /locus_tag="IWK15_000119"
     CDS             106370..108496
                     /gene="pnp"
                     /locus_tag="IWK15_000119"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9545752.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            109006..109272
                     /locus_tag="IWK15_000120"
     CDS             109006..109272
                     /locus_tag="IWK15_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9545753.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            109416..111407
                     /locus_tag="IWK15_000121"
     CDS             109416..111407
                     /locus_tag="IWK15_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9545754.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            111761..113062
                     /locus_tag="IWK15_000122"
     CDS             111761..113062
                     /locus_tag="IWK15_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9545755.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(113192..114562)
                     /locus_tag="IWK15_000123"
     CDS             complement(113192..114562)
                     /locus_tag="IWK15_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9545756.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(114744..115067)
                     /locus_tag="IWK15_000124"
     CDS             complement(114744..115067)
                     /locus_tag="IWK15_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9545757.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(115392..116000)
                     /locus_tag="IWK15_000125"
     CDS             complement(115392..116000)
                     /locus_tag="IWK15_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9545758.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            116330..117046
                     /locus_tag="IWK15_000126"
     CDS             116330..117046
                     /locus_tag="IWK15_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9545759.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            117059..117754
                     /locus_tag="IWK15_000127"
     CDS             117059..117754
                     /locus_tag="IWK15_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9545760.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            117838..118467
                     /gene="udk"
                     /locus_tag="IWK15_000128"
     CDS             117838..118467
                     /gene="udk"
                     /locus_tag="IWK15_000128"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9545761.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        38741 a        21538 c        24944 g        33702 t
ORIGIN      
        1 tttaaatctc gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat
       61 gatactgtta tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt
      121 aatgtttctg ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga
      181 gcaaaaaata atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg
      241 ctttatccca ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag
      301 gtagtcgttg ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt
      361 acccaatttt gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct
      421 ctcccccctt taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat
      481 aggtaaaaat atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt
      541 agaatatgct ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct
      601 ctctcgtagt tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct
      661 tccctccgaa gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc
      721 acatattacc gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca
      781 cgcagacgat atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa
      841 ggtattgtga ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt
      901 gaggttcgta tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat
      961 gacttaaaat caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct
     1021 ttgaaagaat ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta
     1081 gcgacggcta cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta
     1141 ttaaaaggac agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa
     1201 taggtgttgc ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc
     1261 atttctttta ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt
     1321 ctagggattt tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa
     1381 cccctacaaa ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta
     1441 gaaattggaa agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa
     1501 gctctttcag ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca
     1561 ctggcagatg gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac
     1621 tgcgggtatt tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct
     1681 cgaagtgctc gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat
     1741 tggctttccc aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt
     1801 cgctatctct atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga
     1861 cgtcattatt atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa
     1921 tatgaagcca tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta
     1981 ttttgatagg tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc
     2041 aaagccaacg ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt
     2101 ctagtcgtcc tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt
     2161 cagttagata catttttcta tcaacttcat acgccatact tttggagcag tattctcact
     2221 gctctttttg tatgcggact tatcagctgg ctttattatc actatcgtat agattctata
     2281 aaacagttag agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa
     2341 gccgaagaaa ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa
     2401 cgaatccgcc attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat
     2461 gtagaaatca ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa
     2521 acaggattgt attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc
     2581 ctactttatg atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga
     2641 cgtgtcaaat tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt
     2701 gctgggggca ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg
     2761 catacaaatg caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg
     2821 gcggtcatga ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt
     2881 tacgaagata tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga
     2941 gaaaattatg cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca
     3001 atgatggata tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta
     3061 atgttaggac gacaagcagg cttcttttta attcttgcct gtcaacgtcc agacgcaaaa
     3121 tattttagtg atgggattcg ggacaacttt aacttccgtg tggctttggg tcgtatgtct
     3181 gaaatgggct ttggtatgat gttcggaagc gatacacaaa aacaattttt cttaaaaccc
     3241 atcaagggaa gaggttacgt ggatacagga aaaagtgtga ttagtgagtt ttatacaccc
     3301 cttgtaccaa aaaggtacga tttcttagga gaaataggaa aagtcattca aaaaaagcaa
     3361 tctgagcctg taccgcacga agtgaaaggt acaggttcag acttgctttg attggtgtgg
     3421 cgttagccac gccaatcctt tagccccctc tatctaacag gggggcacaa atacaactga
     3481 aaacaggcaa ttctcatcaa ccaatgttga tggtacaaag gattgcggaa aatatgaaag
     3541 gtgtaaactc aagggtcata gtttacatct tttttagatt ggaggtttgc gaatggaaaa
     3601 cgaaaaaatt tggattaagg aattgaaaga aaagcgactg gcttatggcg tttctcaaaa
     3661 taaactggcg gttgcttctc atattaccag accttaccta tcggatattg aaacaggcaa
     3721 ggcagtccct actcaacagg tgaaagaaga tttactgaac gctttggaac gcttcaatcc
     3781 cgataaccca ttggagatgt tattcgacta tgtacggatt cgattcccta ccaatgatgt
     3841 ggctcaaatt atcggtgagg ttttacgttt gaatatggat tatatgctac acgaagactt
     3901 tggctattat tcttatcccg aacactatcg tttcggtgat attgttgtcc tagtctctca
     3961 tgatgtaagt aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc aatttgaaaa
     4021 ctttctattg gctcaacatc ggagctggta tgatttcttt caagattgca tggaacataa
     4081 aggtattttc aaacgcttgg atttagctat caacgataaa acagggattt tgaatattcc
     4141 agatttggca aagaaatgta aacaggaaga atgcatttcc gtctttcgaa gctttaaaaa
     4201 ttatcgcagt ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa acacacttta
     4261 tattggctca ttaaaaagtg aagtctattt ctgtatatat gagaaagatt atgagcaata
     4321 cgtgaaaaat gatgttccct tagaagacgc agaagtaaag aatcggtttg aaattcggtt
     4381 aaaaaatgac cgagccaccc aagccatgaa agatttattg gcacatcaac aggcggaaaa
     4441 aacagccttt gaaattatca atcgctatat ccgctttgca gataaagacg acactaaacg
     4501 tcggagtgat tggaaaacta atgaacgttg ggaatggttt atcggtaaaa atcgtggagc
     4561 attacgccta accacacagc cagagcctta ttcttttgaa cgaacattga actggttaca
     4621 tcatcaagtc gcccctacat taaaaatcgc aagcatttta gatgttctca acggtacaac
     4681 cattatctcc accatgattc aggaagccaa actgacagaa aaacatgaga aattaattga
     4741 acaacaacat ctagccatgg aggatttgat tacatgagcc ttttaacaaa agaattaaag
     4801 aagttagggt ttcagtgtgg cattgagttt caagcgtata tacaaaatac agggaaatat
     4861 acgtctctca ttattgaggg aaaacggcaa gctggtgata ccatctatac ctatgatttt
     4921 tacaaggtga gattttataa taattatacc aatcgagtaa ccgtctatgg agagcattta
     4981 accccttttc aattattaag gcgtgtaaaa agctatatct attatcgaga aaaatatctt
     5041 aaggaaagga ggacaatcac atgaattttg ggcagaattt gtacaactgg ttcttggata
     5101 atgcacagtc attggtattg ttagcgattg ttgtcattgg actgttttta ggttttaaac
     5161 gagaattttc aaaactcatt ggcttcttga ttattgctct aatcgccgtc ggacttgtgt
     5221 tcaatgcagc tggcgtgaaa gatgtgttat taaacttatt taaccgaatc attggtgctt
     5281 aacagatatt tttcatgata aaataggaag tatatatttt gttatgaaag gatttatgaa
     5341 tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa gaaagagctc
     5401 ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat cagaggtaac
     5461 tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct ggaatacgct
     5521 ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg aaagtcataa
     5581 agatttgatt acccagacag caagctccat atatgataaa caaggtgata atgtattagt
     5641 ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta gtcagttgaa
     5701 aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga gtaatggtga
     5761 atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa ttgaaatact
     5821 aaaaaaatat gaaattacag ttcctagaga cgaaaatcta tctaaattat atagtaggat
     5881 acaacaatta attgaaaaag aaattcaacc tactgaactt gctgatatag tgaagacaac
     5941 gataaggtca tcaaatggaa tgattagctc tcttaatgaa gcaagaaata ggcattcact
     6001 tgcacaccct aatacaaata ttattggaaa gagagaagca aaactaataa taggtatttc
     6061 aagtacagtt acagattata ttagcggata ccttgataag tagcgtcatt aactgattgc
     6121 tacttttttt atgactattt ttataaaaag gatgtgataa gaattggaaa tgaaagtcta
     6181 tgtggcaaat ttaggacggt ataatgaagg tgaattagtc ggagcatggt ttactccacc
     6241 aattgatgag gaagaaatgg cagaaagaat cggtttaaat gaggactacg aagaatatgc
     6301 aatacatgat tttgagcttc cttttgatgt ggacgaatac acgccgatta gtgaaatcaa
     6361 ccgtctatgt gaagccattc aagaaatcga aggtacacct atttataatg aactcaaaga
     6421 aattcaaggc atgtggttta gtagcttgga agaattactg gaaaacaaag aggatattca
     6481 ttgttatagt gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg aagaaacagg
     6541 acagcttggt gaagtccctt ccaatttaca aaattatatt gactaccaat ctttgggacg
     6601 tgatatggaa atagagggta attacctagt tacctctcat ggggtatttg aatattgtca
     6661 gtaagtttaa caatccgtgt tataattaaa ttaataaata gcacggagag gtataaatta
     6721 tggtaaatgt atttgaaaat cttaaactat taaaactaga catgaaagat aaaggctggg
     6781 tgatagattc cttttacttt cgttacaaac agcaaaatta tattgtactt gttaagctat
     6841 ttgaaaaaga ggaaaaagtt ccagaatatg ctctgttaaa actagaattt ttaaaggaaa
     6901 acgatttttc agatatgcta gctgtctatg ctaattcggt aaaattatat acagacacga
     6961 aaacaataag agaatatttt ggtatcgaat acagtagtaa cttgggagat gttttatttc
     7021 aatttagcca aactcttgct cgatttattc cgacagaagt tagtgagaag aaaaatgaag
     7081 accaaaaaga agccatgtgt ttctcattaa gtcaaagtga ttcagaagat cccagaaaaa
     7141 aatattgttt ttcagttaga agaaatcctt taaagggaaa tggtgagctg ggaaaacgaa
     7201 gtccttttaa tgacaataaa acgagattgt atagacccag cttatatgaa cgattagggt
     7261 cagataccaa tttaagtttc agatattcaa tgaatccaga tgatgaagaa actgatgaag
     7321 gtattattgc aaaatggaca aaaaataaaa ttgaatagtt aaagcattga aagagcaatc
     7381 acctattttg attgttcttt ttctattttt acggaaagga cgtgaagaca tgaaaaaaat
     7441 aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcgatta atgatttaca
     7501 actccctttc ccaattacct ttacgcaaat gacatggttt atcgtttctt tgtttgtcgt
     7561 catactcttt ggcagtttac caccactttc tctgattgac ggagcactac tcaaatactt
     7621 agggattccc attgttctca ctgggttcat gtgccaaaag acctttgacg gaaagaaacc
     7681 ctatggattt ctaaagtcta tcattaccta tgctctacgc cctaaagtaa cctacgctgg
     7741 caaacaaata cgattaaaaa aagaaacaat gaatgaatcg attacgattg taaggagtga
     7801 acaactagat ggcatatccg attaaatata tggaagataa tctcgtattt aatcatgatg
     7861 gcgaagtatt cgcttattat gaattaattc cctataatta cagtttttta agttccgatg
     7921 aaaaaattca agttcatgat aatttctgtc aattgattgc ccaaaatcgt gatggcaaga
     7981 ttcatgctct gcaactagca accgaatcaa gtattcgagc cacacaggaa caatcgaaaa
     8041 aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa cagaccgaag
     8101 cgttaatttc tatgattggt gacaatcaag tagactatcg cttttttatt ggttttaaac
     8161 tctacctcaa tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt caaaaagcat
     8221 ttgcggaatt ctttcatggt gtgaatcaca aattaatggg cgattttgtt tctatgccta
     8281 tggcggaaat cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa ttatccagac
     8341 gctttcagat tcgtaaattg gagaaagatg attttggtta tattattgaa catctttacg
     8401 ggcaaacagg tgtccctttt gatgactacg actaccattt accgaaacga tacgaagata
     8461 aaaaagtgtt ggtaaagaaa tacgatattc tcaaacccac tcgctgttta atcgaagaaa
     8521 atcaacgcta tttacggatt gaacaggaag ataccactac ttatgtggcg tattttacaa
     8581 ttaatagtat tgttggtgaa ttggatttcc ctaatagtga aattttttat taccagcaac
     8641 agcagtttac tttccccgtt gataccagta tgaatgttga aattgtcgcc aataaaaaag
     8701 ccttatctac cgttcgcaat aaaaagaaag aactgaaaga tttagatagc cacgcatggg
     8761 aaagcgacaa cgaaaccagt actaatgtgg tggacgcttt agaatctgtc gatgaattag
     8821 aaagtaattt agaccaaacc aaagaatcta tgtacaaact aagctatgtg gtacgggtat
     8881 cagcaaagaa tttggacgag ctgaaacaac gttgtgatga agtaaaagat ttttatgatg
     8941 atctgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat aatgaattta
     9001 ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg gattttttgg
     9061 ctggtcttgg tttcggtgct acacaaatga ttggtgaaaa ttctggtatt tacgtcggtt
     9121 acaatctcga tacaggcaga aatatttatc tccaacctag ccttgcaagt caaggaatta
     9181 aaggctctgt aacaaatgca ttagcgtccg cttttattgg ttcattaggt ggcggaaaat
     9241 cattctctaa caatatgctg gtctattatt cagtactctt tggcggacag gcaaccattg
     9301 ttgacccaaa agctgaacgt ggtgaatgga aagaaacctt acctgatatt gcccatgaaa
     9361 tcaatattgt taatctcacg agtgagaacg acaataaagg gttacttgac ccttatgtga
     9421 ttttaaaact cccgaaagat tcagaaagcc ttgccattga tattctcacg ttcttaacag
     9481 gtatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagctatt cggagtgtaa
     9541 ctcaaagcaa acaacgaggg ttgttattgg tgattgatga attgcgaaaa gaagatacgc
     9601 ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat tttgcccatc
     9661 tgttattcag tgatggcagt gtcacccaat ccattagctt ggaaaaacaa ctcaatatta
     9721 ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa tacactacca
     9781 tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta gactttatcc
     9841 actctgaccg ttccattttt aaaattgttg accttgatga ggcttggagt tttttacaag
     9901 tggcacaagg aaaagctcta tctatgcgat tggttcgagc aggacgtgcc atgaatgcag
     9961 gggtctattt cgttactcaa aacgcggacg atttactaga tgaaaagatg aagaataata
    10021 ttggcttgaa atttgccttt cgttccactg acctagtgga aatcaaaaag accttagaat
    10081 tttttggtgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa ttagaaaatg
    10141 gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt cacccgattt
    10201 ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag gtggaagaat
    10261 gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt gtgtgggcgt
    10321 tattttactt ttattaggca cgctgggcaa tgcagtacaa gcgacaggct tagttgatga
    10381 aaccattgat acaagcaatg aatactccaa gtatggtttg aatcaatatc agcttgatta
    10441 ttatgtggac aatagctggg gctggcttcc atggaattgg agtgatggca taggacaatc
    10501 ggtgatgtat ggattgtatg ccattactaa ttttatttgg acaatttctc tttatctatc
    10561 gaacgccaca ggttatttgg tgaaagaagc ctatgcctta gatttcatta gtcaaacatc
    10621 ccaagcaatt ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg gcttttccag
    10681 ttcagggttt tatgtcggtt tcctccttct ttttattctc attgttggaa tctatgttgc
    10741 ctatacagga ttggttaagc gagaaacgac gaaagtgata cgagctgttt taaactttgt
    10801 cgtggtgttt attctatcgg cttcctttat tgcgtatgca cccgattata ttgcgaaaat
    10861 caatgatttt tccaaagatg tcagtaccgc cagtctgaat attgggacaa aaattgttct
    10921 tcctaacacg gatagtcaag ggaaagacag tgtggacatg atacgtgaca gcttattctc
    10981 tgttcaagta aagcaaccat ggctattgct tcaatatggc tccactgatg tagaagcgtt
    11041 aggggaagaa cgtgtggaaa gtttattgtc taccagtcca gatacaaaca atggtgaaga
    11101 tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt taaccctacc
    11161 gaagacgatt tccagattag gaacagtctt ctttttgttt gtattcaata ttggtatctc
    11221 gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat ttattatttt
    11281 cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg atggcatggg
    11341 caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta ttaccttgat
    11401 tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa gttctccctt
    11461 tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta aactgggcga
    11521 tttaatgagc ctattcactt tacaaagcaa cgactctcaa aacatgggca gacaagtgtt
    11581 tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca gtatgagtaa
    11641 gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa agcagaatca
    11701 aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga aagaaacagc
    11761 actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg aaaagacagc
    11821 ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcaa aacaacagat
    11881 acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga atgtcactca
    11941 taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca aacaacagcg
    12001 tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg agctggataa
    12061 agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata accagcagca
    12121 tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta aacgtgaaaa
    12181 tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc aattcaatca
    12241 gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct aaaaataaca
    12301 gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct agtcattttc
    12361 tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctattcc acaaggcggt
    12421 gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata tggtaaggaa
    12481 tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga atcgggcggt
    12541 acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa cagtttgagt
    12601 accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac cagtgcagaa
    12661 caacaaggag tggatataga ttctgttatc caatcttata attatggcgg tggcttttta
    12721 aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca gttttctaaa
    12781 gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc tgtgaatggt
    12841 ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca atacctaacc
    12901 gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga cgaagccttg
    12961 aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc ttttgattgt
    13021 agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg tgtcgcccaa
    13081 gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg cgaccttatc
    13141 ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat atatttagag
    13201 ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag cagtcgttat
    13261 tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag gaatgaatca
    13321 aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat ctaaaaaacc
    13381 gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat taacctttgt
    13441 tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt ttactgccat
    13501 tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg ataccaacca
    13561 agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac aatcccaaga
    13621 agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat tgcaggtatt
    13681 aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg atatacaggt
    13741 gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg ttgaacaagt
    13801 tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg ttgtccatat
    13861 agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga aacctcaaaa
    13921 atcggactac caacccaaac agctagaaag tgaccataca gtagatacag aaacgatgga
    13981 cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca cagaaaaaga
    14041 attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat atgttttcga
    14101 ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga atgtcgctgt
    14161 gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt tgatattaga
    14221 aaaacaagat aattggaaga ttgtaaaata acaaatattg gtacatgatt acagatactt
    14281 tgtaatcatg tactcttttt gataaaaaat tggagattcc tttacaaata tgctcttacg
    14341 tgctattatt taagtgacta tttaaaagga gttaataaat atgcggcaag gtattcttaa
    14401 ataaactgtc aatttgatag cgggaacaaa taattagatg tcctttttta ggagggctta
    14461 gttttttgta cccagtttaa gaataccttt atcatgtgat tctaaagtat ccagagaata
    14521 tctgtatgct ttgtatacct atggttatgc ataaaaatcc cagtgataaa agtatttatc
    14581 actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta
    14641 atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat
    14701 ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata
    14761 atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt
    14821 gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag
    14881 tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg
    14941 tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct
    15001 tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta
    15061 aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt
    15121 gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc
    15181 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
    15241 gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata
    15301 tagggattga taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag
    15361 gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc
    15421 ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac ctaaatgagt
    15481 atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa
    15541 tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa ataattattg
    15601 taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt
    15661 ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat tgggaaatat
    15721 cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt actgaaagcg
    15781 aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt gaatggattg
    15841 ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat agagtgggtt
    15901 gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt gtagatgtta
    15961 aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat ttattaactc
    16021 caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct tttaaatgga
    16081 acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt tgtaattttg
    16141 ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat aaaatgactg
    16201 gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct attggcagag
    16261 gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt gaagaaatta
    16321 aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa tttcataaag
    16381 aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg
    16441 ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag ttcactaatg
    16501 ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc attcttccaa
    16561 tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat attttaggaa
    16621 ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt ttggagtttt
    16681 tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg tggcttaata
    16741 cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat
    16801 atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct gaggtcttat
    16861 caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg gatgaattaa
    16921 atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt aaaaatgata
    16981 ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa aataggaatg
    17041 cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat ggtttaaatg
    17101 ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg gttaatgccg
    17161 acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt tatagagtat
    17221 tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt aacaagacga
    17281 aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg ctcgattggg
    17341 gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat tatctaactg
    17401 aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga gaaagatatc
    17461 gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa ggattttatt
    17521 cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca aagatgctat
    17581 taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt tatatatcat
    17641 ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt tacgctaact
    17701 tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac aacttttgtc
    17761 gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta gggattacag
    17821 ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag cttggagtaa
    17881 tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc cgttaactca
    17941 ataagttttt tctttcatat acatgaaaat agtagttata cggattttta tcattcttta
    18001 tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga aaaaaagtat
    18061 ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa gggaaaaaca
    18121 aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta cataactcaa
    18181 aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc ttatctctcg
    18241 tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg ttcttcctac
    18301 ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc ttgggaatcc
    18361 tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca gcaatcaaag
    18421 aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc ttatttctat
    18481 caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt tctataagtt
    18541 atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt caattgcatt
    18601 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
    18661 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
    18721 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
    18781 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
    18841 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
    18901 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
    18961 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
    19021 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
    19081 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
    19141 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
    19201 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
    19261 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
    19321 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
    19381 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
    19441 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
    19501 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
    19561 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
    19621 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
    19681 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
    19741 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
    19801 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
    19861 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
    19921 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
    19981 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
    20041 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
    20101 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
    20161 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
    20221 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
    20281 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
    20341 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
    20401 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
    20461 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
    20521 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
    20581 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
    20641 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
    20701 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
    20761 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
    20821 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
    20881 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
    20941 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
    21001 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
    21061 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
    21121 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
    21181 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
    21241 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
    21301 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
    21361 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
    21421 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
    21481 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
    21541 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
    21601 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
    21661 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
    21721 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
    21781 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
    21841 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
    21901 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
    21961 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
    22021 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
    22081 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta ttattataaa
    22141 actatttgat ataattatag gtgaatattg gagttttcct agcctataat tgggaaagga
    22201 gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg aacttctttt
    22261 tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca tatctactat
    22321 caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct aggaattcgg
    22381 ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa actatggtta
    22441 aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat ctaggttatg
    22501 attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa aaagaagcaa
    22561 gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag aataaggata
    22621 aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag acacaggtaa
    22681 agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct tattttgata
    22741 aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta aaattatcaa
    22801 agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt gctatggatc
    22861 gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc aatgtgaaaa
    22921 agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta atctctctaa
    22981 tttacaaaga agattactat caacactttt tatttatctc tctgtggttt ttatttatga
    23041 ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat tttgactcag
    23101 gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat agatttgttg
    23161 aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca ttaacttatt
    23221 tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta atgagctata
    23281 acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat gctaagctgg
    23341 ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc ttgttaatca
    23401 gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat attgagacga
    23461 ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct aacaaattaa
    23521 atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca aagaatcaat
    23581 ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa
    23641 agataagaag agccagaaac ccttggtata agaggatttc tagctctcct acaactattc
    23701 ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc
    23761 tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa
    23821 gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt
    23881 acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca
    23941 gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg
    24001 tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc
    24061 agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg
    24121 gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg
    24181 cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat
    24241 aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt
    24301 tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc
    24361 tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga ctttgatgat
    24421 gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag
    24481 cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac
    24541 cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac
    24601 ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc
    24661 acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg
    24721 tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat
    24781 cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc
    24841 accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc
    24901 gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc
    24961 gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa
    25021 attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc
    25081 agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct
    25141 caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg
    25201 actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa
    25261 atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct
    25321 aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat
    25381 cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc
    25441 atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat
    25501 aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt
    25561 tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt
    25621 agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag
    25681 gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag
    25741 gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca
    25801 tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat
    25861 ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag
    25921 acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga
    25981 tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga
    26041 acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat
    26101 ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt
    26161 gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt
    26221 agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt
    26281 aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc
    26341 tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata
    26401 gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga
    26461 acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt
    26521 tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt
    26581 cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg
    26641 atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat
    26701 ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat
    26761 cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc
    26821 agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac
    26881 aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca
    26941 aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac
    27001 aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg
    27061 gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt
    27121 tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag
    27181 tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc
    27241 ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt
    27301 gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga
    27361 ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga
    27421 ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg
    27481 aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca
    27541 tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag
    27601 agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag
    27661 aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg
    27721 ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc
    27781 caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg
    27841 aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg
    27901 gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc
    27961 atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga
    28021 ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta
    28081 ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca
    28141 aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca
    28201 tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac
    28261 gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa
    28321 caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat
    28381 tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt
    28441 tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga
    28501 tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa
    28561 agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt
    28621 cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt
    28681 aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa
    28741 ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct
    28801 agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg
    28861 tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga
    28921 atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc
    28981 accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc
    29041 gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt
    29101 ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc
    29161 aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga
    29221 tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga
    29281 aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca
    29341 aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga
    29401 agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg
    29461 agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct
    29521 tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc
    29581 aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt
    29641 gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta
    29701 tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg
    29761 tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc
    29821 ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca
    29881 tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa
    29941 aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca
    30001 attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac
    30061 acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga
    30121 tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat
    30181 cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc
    30241 gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga
    30301 ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt
    30361 gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga
    30421 agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt
    30481 agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg
    30541 gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt
    30601 ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa
    30661 ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt
    30721 ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc
    30781 cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct
    30841 gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca
    30901 ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac
    30961 tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat
    31021 ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg
    31081 gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca
    31141 cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa
    31201 aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt
    31261 caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga
    31321 tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag
    31381 gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa
    31441 ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa
    31501 caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg
    31561 gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga
    31621 atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct caaggtatgt
    31681 atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac ggtgtattcc
    31741 aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc
    31801 gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac ttcacattga
    31861 catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa tcagcggacg
    31921 gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt gttggtctaa
    31981 cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca
    32041 tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc
    32101 ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg ttcatttatc
    32161 caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg tttgtttcaa
    32221 aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata gaaatcgtta
    32281 ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa tattaccaaa
    32341 gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc aggagaaatt
    32401 catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt gctttccgga
    32461 ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa gatcacaagt
    32521 ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat gcttgttgat
    32581 gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt
    32641 gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg attgtcagtc
    32701 aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt agaaatttta
    32761 aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc tgtattgacc
    32821 cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg
    32881 atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg ctgtacagtt
    32941 atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc acaacaatta
    33001 gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct
    33061 caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc
    33121 gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat
    33181 ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc agaaagcgga
    33241 catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat cactgaacat
    33301 ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga tatgacatta
    33361 tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa cggcatgctg
    33421 aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga tgttcgaaca
    33481 acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa agcaattatc
    33541 gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac tcgtggactg
    33601 gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga caaatacaaa
    33661 gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga tcgtattgct
    33721 gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa
    33781 ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt agaaaagggg
    33841 gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc ctgtactttc
    33901 agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt ttcagcccat
    33961 ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga taggtgaaac
    34021 gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag ctaactcagc
    34081 tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg tcagtgtctg
    34141 ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat gcgtgatctt
    34201 aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag cattttttgg
    34261 aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg taagcacatt
    34321 tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt ccaatatgat
    34381 cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg gctctcgcgt
    34441 aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc tgatgaagaa
    34501 aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt ctgagtatgc
    34561 aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg ctttggcagg
    34621 gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc aaacgacttc
    34681 gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat cagcaatcgg
    34741 tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg gcatgcagac
    34801 aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta tcttctttgt
    34861 cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga
    34921 agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact
    34981 ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt ttattcaaca
    35041 ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat cgtaaacgta
    35101 gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa cttgacgatg
    35161 agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt
    35221 atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga ccatattatc
    35281 agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat caaagcatgg
    35341 tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc tttcccagga
    35401 ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt acctgcttat
    35461 tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa attcggcttg
    35521 cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat caatgtatat
    35581 gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg cggtgctgtt
    35641 tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca agggttcatc
    35701 tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg tgcctcattg
    35761 ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt tatttcttcg
    35821 attccgccag tattgctgca aattgctcca tatttgctga caatcattgt gctggttgtt
    35881 ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa atcaaaataa
    35941 tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt
    36001 ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc gtacatctga
    36061 tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc ggtgatgttg
    36121 gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca catttagagc
    36181 agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag
    36241 gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca ggccactggg
    36301 aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg
    36361 atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac aaaccttata
    36421 gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc gatttaattg
    36481 tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc attcctttag
    36541 aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat ccgtatatga
    36601 ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca
    36661 atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct ttgaaagaag
    36721 caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga caaggaatca
    36781 ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg aaagttatga
    36841 aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca ttatacggtc
    36901 atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta cgaattccag
    36961 aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat cacggaaacg
    37021 atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg gtttacagta
    37081 aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt tcagcaacga
    37141 ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg aaattattaa
    37201 aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt tacaagaaac
    37261 gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga ttctaggttc
    37321 aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt atgatgagat
    37381 cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt atgggtctct
    37441 ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag gtcattccat
    37501 gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt cagtcattgt
    37561 gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga tgctgattac
    37621 cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg
    37681 ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc
    37741 agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct attctggacc
    37801 aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg atgcagtagg
    37861 gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag tgttaggaat
    37921 ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg aagaagttgt
    37981 agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa cattgacatt
    38041 attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg
    38101 ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg gagacttttt
    38161 tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc ggcaaatata
    38221 aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc accatctatg
    38281 cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt acatgcggtg
    38341 cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca gcgaccagtt
    38401 cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt gatttcaatc
    38461 tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat gtaggaaatg
    38521 ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa ttagctgaac
    38581 ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc gcgaaatacc
    38641 atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt gaagaaacaa
    38701 cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg gaaacagcga
    38761 tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga acatccaagc
    38821 aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg taagcttttg
    38881 aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt
    38941 tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgaga agttggcagg
    39001 agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc ccttttttat
    39061 agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa ttcataaaca
    39121 agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat accttcgata
    39181 tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt tttgttgtct
    39241 agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa tgtcactttc
    39301 aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg gtcatttgct
    39361 gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga acgacgccag
    39421 atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa accttgtaat
    39481 ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca aagttgatct
    39541 gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga agtgtaagca
    39601 acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt
    39661 ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc attccattca
    39721 ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt taagataggt
    39781 ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag agatttccat
    39841 cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa acgaacagtg
    39901 cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc tttttcaggt
    39961 aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga aataaattgg
    40021 atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt tgcatcgatt
    40081 tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac taatgtacgt
    40141 tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa atagattttt
    40201 cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc gcgatctttg
    40261 aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg aagttggcaa
    40321 atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg aacagcagta
    40381 gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc aaagtcagat
    40441 aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata
    40501 attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca gtaaaaatgt
    40561 agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct ttttttattg
    40621 gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc aacgcctttt
    40681 tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc tactacccca
    40741 acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc tagatgcaga
    40801 tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc tgtacgcaag
    40861 acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc ataagggatc
    40921 acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga atcaacgatc
    40981 caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc agcgccgccg
    41041 cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt caaatcgact
    41101 acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg ttcttttgtc
    41161 gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc
    41221 tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat cccgtccttt
    41281 acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc gtccaccttt
    41341 ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt aaaaagatac
    41401 cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt
    41461 ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg
    41521 ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat tttttatcac
    41581 aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg aggaaacaag
    41641 gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta
    41701 attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact aacgtaaact
    41761 cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa aaaccgaact
    41821 cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt actgcttata
    41881 tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc ggtggtcgtg
    41941 taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat actgctggtg
    42001 ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa gcttaatttt
    42061 aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat gaacaatatt
    42121 tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc
    42181 agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg
    42241 aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca aagaatctac
    42301 aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga
    42361 agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag ttcgccctga
    42421 acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca
    42481 cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca caggtgcttc
    42541 tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta
    42601 tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag atagtgtcct
    42661 tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca gagaattttc
    42721 tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta aaacgactgc
    42781 tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa ctcatgaagg
    42841 agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga tcacttctgc
    42901 tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac caggacacgt
    42961 agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg taactgttct
    43021 tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag caacaactta
    43081 tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg acttcttata
    43141 ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc aattaccaat
    43201 tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg ctgaaatgta
    43261 tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat accgcgaatt
    43321 agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg aagaacttac
    43381 tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag gtatccgccg
    43441 cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta agaacaaagg
    43501 ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag atatcccagc
    43561 tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag acgacagcgc
    43621 tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc gtttaacttt
    43681 cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg caacaaaagg
    43741 caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt cagaaatctc
    43801 tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa ctactgggga
    43861 cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc cagaaccagt
    43921 tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt
    43981 acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga
    44041 aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc gtatgcgtcg
    44101 tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg aaactttccg
    44161 tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg
    44221 tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc
    44281 gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg gtttgaaaga
    44341 tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag ctaaacttta
    44401 cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat
    44461 ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa tcatggctgt
    44521 agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg
    44581 tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc gtgcgatggt
    44641 tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc aaggtcgtgg
    44701 tacatttaca atgacatttg accactacga agatgtacca aaatcagtac aagaagaaat
    44761 cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt cctgcaaaga
    44821 gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta tcgatgatat
    44881 gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa aatatattgt
    44941 atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta aaccacacgt
    45001 taacattggt actatcggac acgttgacca tggtaaaact acattaacag ctgcaatcac
    45061 aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa tcgatggtgc
    45121 tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt atgaaacaga
    45181 tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta aaaacatgat
    45241 cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg acggcccaat
    45301 gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt
    45361 attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag ttgaaatgga
    45421 agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg tagttgctgg
    45481 atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat
    45541 ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac cattcatgat
    45601 gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag gtcgtgttga
    45661 acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc
    45721 aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg
    45781 agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt
    45841 tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag tatacgtgtt
    45901 gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc cacaattcta
    45961 cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg aaatggttat
    46021 gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta tcgaaaacgg
    46081 tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg ttacagaaat
    46141 caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg taatcgctcg
    46201 gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga tcaggaaaaa
    46261 agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt ttctaaatga
    46321 aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga gtttatatat
    46381 attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc agaatgattt
    46441 cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga acgagcgtga
    46501 cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg
    46561 caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag
    46621 cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt ccaattccat
    46681 taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac aaagattcac
    46741 gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac ccaacaccaa
    46801 aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc gaaatcaaat
    46861 tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc ttagggaaaa
    46921 aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta actgttgtag
    46981 aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc tacgaagcta
    47041 ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg
    47101 ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat gttgagctag
    47161 gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga gacattattg
    47221 atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa
    47281 gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca atgggtccag
    47341 ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg
    47401 taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca
    47461 aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct gtgaaagcta
    47521 aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta ttcaaacaag
    47581 atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt gaacctaatg
    47641 aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg
    47701 cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa
    47761 caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt ggcgtagtct
    47821 ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt cgtttagcaa
    47881 tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc gaaggcttga
    47941 acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta tctatcgact
    48001 ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca gcacgtaact
    48061 taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt gtatcaaata
    48121 caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt gcataatgaa
    48181 cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg ccatggatga
    48241 caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa aacaagcggt
    48301 agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt
    48361 caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga tcgttacatt
    48421 gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac taatagataa
    48481 ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac ctcaaatggt
    48541 cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc tgaaaaatca
    48601 ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat cactgtacgt
    48661 catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg taacaaagat
    48721 aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc taacatcgcg
    48781 ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg actagaagtg
    48841 ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc tcttccatta
    48901 gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg taaaggtgga
    48961 caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg caaatacgta
    49021 ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg tgctacaatc
    49081 ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg acgctctcgt
    49141 tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga tcacccacac
    49201 ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa
    49261 ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact tattgttcgt
    49321 cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag gaggttcacc
    49381 atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa aaaagttgaa
    49441 gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt
    49501 ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca tgtaccagtt
    49561 tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac aagaacttat
    49621 cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag gataaatcca
    49681 aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca cttcacctcg
    49741 aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg caatttcaat
    49801 cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga tgtcagcagt
    49861 tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt
    49921 tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc
    49981 aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag gagggataat
    50041 cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc gactgggatg
    50101 ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta agaatccgta
    50161 aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa
    50221 caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt ggtaaaggcg
    50281 gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa agaatccata
    50341 tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt gaaggaatcg
    50401 cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc atccaacgct
    50461 caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag
    50521 atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact ttacgtgcgg
    50581 acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga gttaaagtgt
    50641 ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga gggaaataat
    50701 catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga
    50761 agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg ttgattcaca
    50821 ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg
    50881 tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca aagcaattgg
    50941 ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg
    51001 taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag cgttacgtct
    51061 agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga
    51121 atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt gatcaagaaa
    51181 aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt caattagaaa
    51241 acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg
    51301 aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga agaaatcaac
    51361 gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt accgttgctg
    51421 tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct aagaaataca
    51481 aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt atggaaactc
    51541 gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa gcagttatta
    51601 tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg aggatacaca
    51661 acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt
    51721 tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt gatgtgatcg
    51781 ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag
    51841 ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat attaaatttg
    51901 atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca cgtatctttg
    51961 ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag
    52021 aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg tttgttaaaa
    52081 aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc gttgtactag
    52141 cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc
    52201 aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg ccaattcatg
    52261 tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc tacaaagaag
    52321 tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat aaataaaagt
    52381 ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa
    52441 ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct aaagttgaaa
    52501 aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac ttagataaag
    52561 cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa gctaaaaaat
    52621 caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg
    52681 gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca cgtgtacgtg
    52741 acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact ttaggtatta
    52801 aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta cgtggtatgg
    52861 acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg ttgacacaat
    52921 taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa aaatcaatga
    52981 ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt
    53041 gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac
    53101 ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc
    53161 ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt ttctaactcg
    53221 tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat caaaaattaa
    53281 acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga
    53341 agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt
    53401 tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca aagctgacga
    53461 agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt
    53521 cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt acgtatggta
    53581 atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg
    53641 ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt aaaggaccaa
    53701 aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag
    53761 taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga acaactcgtg
    53821 cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa
    53881 tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac gttggttact
    53941 cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca gctaacacac
    54001 aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca aacattcgtg
    54061 gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc gaatatgtac
    54121 gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag aagccagcag
    54181 caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc
    54241 agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa aaataaagag
    54301 gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag acatcgtcgt
    54361 gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt tcgttctaac
    54421 aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc aagtgcctct
    54481 accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa
    54541 ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga ccgtggtgga
    54601 tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa
    54661 ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat ttggaattag
    54721 aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac
    54781 gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt ggtacaggta
    54841 aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag aaaaacttag
    54901 ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct tttagtggtg
    54961 gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt ggaccagttc
    55021 gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta ggctcaaata
    55081 caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag
    55141 aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga ggacaacaat
    55201 aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg
    55261 cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta agccttctaa
    55321 tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg aagaaattta
    55381 atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa cttcatgaat
    55441 taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg
    55501 gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg
    55561 tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca aaacgaggat
    55621 tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg aaccgctttg
    55681 aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa
    55741 aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact gtgaaagcag
    55801 caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca atcgaggtga
    55861 tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat cgaaaatcct
    55921 atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg taccaggcgt
    55981 aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt tgaacttggt
    56041 aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac catacattac
    56101 tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc
    56161 gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc tgacattagt
    56221 attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg ctcaattagg
    56281 tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc taacagccgg
    56341 tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg gaaatggcgt
    56401 atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca gagaaattta
    56461 tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta tttttgtggc
    56521 cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc aacaagctga
    56581 acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa gcagctatct
    56641 gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt cgtttattgc
    56701 tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa actggtacaa
    56761 agtgatgatg gagatcttta actataatac agcacctggt gctacgattt atacgattct
    56821 gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga aattagcgga
    56881 aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg ggactgaaga
    56941 gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc ttggtttagt
    57001 ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa tcggattagg
    57061 cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac agttagaagg
    57121 cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc
    57181 cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc attttaatgg
    57241 ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct tatggtattc
    57301 cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca gctcttggcc
    57361 tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta acaaatggaa
    57421 tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta gatggctttc
    57481 cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg aataaaaaaa
    57541 ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt ctagccggcc
    57601 gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct acaaaagttg
    57661 aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat gataaacctg
    57721 agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt
    57781 ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat gctgtatttg
    57841 cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact tgcattatgc
    57901 agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac ttctagagaa
    57961 tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc gccattttat
    58021 cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga agatatgatt
    58081 gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa
    58141 aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta catccgtatc
    58201 ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg tggccgtatt
    58261 acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc atgaaagtaa
    58321 gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta
    58381 tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg gaggtgctat
    58441 aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag tagtttcact
    58501 tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg atgctggtgt
    58561 atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta tccgtgcaga
    58621 agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg
    58681 tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg
    58741 acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta tcgctggcaa
    58801 gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg
    58861 taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt ctacattcaa
    58921 taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt catcagctgg
    58981 ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga
    59041 aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa cagttaaagg
    59101 acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt tagaagtgac
    59161 tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa aacgccgtcg
    59221 tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac aagacacttt
    59281 tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa
    59341 ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga gggtatggga
    59401 ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt gctgccatca
    59461 ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt gttagagaag
    59521 acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat ggccaagaag
    59581 aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc gatattatcg
    59641 ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc tctgaaggag
    59701 ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa
    59761 ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac acacctgttc
    59821 gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat tttgataaat
    59881 tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg
    59941 cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg
    60001 ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg
    60061 aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga atcaatactg
    60121 tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac ttaggacgta
    60181 aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta cgtaaagaag
    60241 attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta ggacgcacat
    60301 ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc aacgaacgta
    60361 ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt
    60421 taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag
    60481 ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta caaaaactgt
    60541 tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc attttgaaga
    60601 cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca
    60661 ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga agatattttt
    60721 ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa gcactcatca
    60781 taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga aggcataaaa
    60841 aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg ttagtaggaa
    60901 ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa gataaattat
    60961 aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac aattgataaa
    61021 ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact tgccaaaacg
    61081 atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca aatattggat
    61141 gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa tccagacaat
    61201 cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa tcaagggatt
    61261 cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag aatggctgag
    61321 tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc
    61381 ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag tatgctagat
    61441 ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga aagtcaattg
    61501 accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg gatactagtg
    61561 atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc tgctggtcct
    61621 gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag cgcactgaaa
    61681 gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt ggattggtta
    61741 tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca tttgaacaaa
    61801 gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca ttagtagggc
    61861 ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt aagccaacaa
    61921 gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat aagaatttaa
    61981 agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag ctattcgaag
    62041 aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag
    62101 cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc tatttggaac
    62161 gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg
    62221 ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat ccccagggca
    62281 gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc actattgtat
    62341 tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat gtgttggaaa
    62401 agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt gaatggctaa
    62461 aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta atggctaaag
    62521 ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct atcgtccatt
    62581 atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat gcctggagat
    62641 tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta ttttatcgga
    62701 atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt cacattactt
    62761 tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg cccattgatc
    62821 tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg agaaacttat
    62881 tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct ctttattttc
    62941 tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac accgcctttg
    63001 gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg gttcccagtc
    63061 cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt gatggacgaa
    63121 acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga aggaaaccta
    63181 gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag cagcttcaac
    63241 cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga
    63301 acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat tgcttttgta
    63361 ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc gctatctatt
    63421 cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa gaaatattag
    63481 ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa
    63541 aattttatag aaaaatcatt attccagata accttttagg aacaaaggag agaaaaacat
    63601 ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat tccaaaaaca
    63661 gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg
    63721 aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg cgatcgggca
    63781 agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga
    63841 tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag aagacttcca
    63901 cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag ggaaaccacg
    63961 cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt tggaaaaaat
    64021 tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt gtgcatcagg
    64081 aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg taaatgaaac
    64141 aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga tgatacggat
    64201 cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata gtatcccaga
    64261 tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg aaggacttta
    64321 tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct tcaagaaata
    64381 agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt attttcgaag
    64441 aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt aactctgagt
    64501 cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg acttgtggac
    64561 gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc aaaaaacgat
    64621 ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat cgtttcttta
    64681 ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg gatcaatcgt
    64741 gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca atgcaataca
    64801 taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga aagtgacagt
    64861 atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac
    64921 tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg tcaccaacat
    64981 ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt ccatttcgac
    65041 acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta gaccaacgac
    65101 tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa atgtcactgt
    65161 tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga tttgcgtttt
    65221 tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg caaaattggt
    65281 tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc aagtgagctc
    65341 ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt
    65401 cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt catttagacc
    65461 agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg gctgctcaac
    65521 atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat tctgatacag
    65581 tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg tgctttcgtt
    65641 tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat tattgttata
    65701 acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt aagaggaaga
    65761 attacgattt cttatagaag aaaacaaact gataccactg taaccatagt tgatcgaatc
    65821 gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat ctttttttgt
    65881 ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc gggaagcata
    65941 attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg cttcagcaga
    66001 catgatcaat aataacaata tactcagatg attgaatgaa agttttttat gctgataaaa
    66061 tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg aagtcatcgt
    66121 cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga gagcagctac
    66181 gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt cccatccttt
    66241 tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata aaaacatatt
    66301 cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac ttgcgataag
    66361 tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc gactggcaaa
    66421 ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa tcgatccgta
    66481 ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat aagaagtcgc
    66541 ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa gatccagcac
    66601 tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga taccataagg
    66661 aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa aatataagaa
    66721 agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga gtaaataact
    66781 gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca aaatcactaa
    66841 tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag tgataaatga
    66901 gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct tgaacgtacg
    66961 tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca gaacatacag
    67021 tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag
    67081 atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg tataaaaaat
    67141 actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa actgtgaaaa
    67201 ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac ttagataaac
    67261 aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc cgttttctct
    67321 aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta tgaagttgta
    67381 ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa
    67441 taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa
    67501 gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac cgttcactat
    67561 ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt tcaaagcacg
    67621 agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt tttgtaagtt
    67681 aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc cactttttgt
    67741 cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg ccttgatttg
    67801 gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg ggaatttgca
    67861 agtacatttt catagataca attgacaatt gagaagaata attgtaaatt aaacaaggtt
    67921 atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta tttcaagttg
    67981 cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa ggaggctcat
    68041 tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat gatgtaattg
    68101 caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa
    68161 atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca agaggagtag
    68221 ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc agctatggta
    68281 cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat
    68341 tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt gtactgccag
    68401 actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt atctaggtga
    68461 gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc
    68521 tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca gatggcgata
    68581 cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta gattcaacct
    68641 cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg
    68701 gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa attccagatg
    68761 ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt gaaactgcgt
    68821 ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga gaagcagcga
    68881 aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg acagctgttg
    68941 taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc ctaaaagaag
    69001 tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc tttttaagtg
    69061 cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca gaaatggatg
    69121 atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca gaagatatcc
    69181 attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca gtcgatagta
    69241 cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct ttacttgtgg
    69301 tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga gaagtcttga
    69361 attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg cgcttgcagc
    69421 atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac
    69481 gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa
    69541 gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt acggaagaca
    69601 ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca aacaataaga
    69661 acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta gcagttgttc
    69721 cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat caagcttctt
    69781 tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca
    69841 ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga gactttttgg
    69901 gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca gcgttaagta
    69961 cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca ggtgatgaag
    70021 gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat cctgatttgg
    70081 aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct gctgaataat
    70141 ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt cggttagtga
    70201 tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa aatttatgcg
    70261 ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag cagctagtct
    70321 tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt ttcgctacga
    70381 agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg
    70441 aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc gattgcaatt
    70501 tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac cttatctaaa
    70561 agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa
    70621 agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt cgccgatata
    70681 tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa aagggtttca
    70741 agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga aatatcgact
    70801 gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg aagaaaacca
    70861 tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc gtcttcaagg
    70921 attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg atgtccaaaa
    70981 attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa aacgtgtaac
    71041 gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga
    71101 tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg ttacagcagg
    71161 tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata tggaaagctt
    71221 gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt caacgaaagc
    71281 aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg
    71341 cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag ttattacgga
    71401 tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc
    71461 agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta cagcatatgg
    71521 tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga tcgaaacacg
    71581 ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag aactggcaag
    71641 aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat tagatgtaaa
    71701 aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat atgaagttgg
    71761 cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag ccttcaaaga
    71821 caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa
    71881 cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc tgcaccaact
    71941 tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg ctaatcctaa
    72001 aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg gttttgtact
    72061 aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg
    72121 tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg aggtagccag
    72181 agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg agtatcagcc
    72241 tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt agcagactgt
    72301 gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc
    72361 aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga
    72421 ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga aatacttaac
    72481 aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg atgatgagcc
    72541 agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac aagcagtgaa
    72601 aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac tagcagcagg
    72661 gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc
    72721 agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta acgcagaaaa
    72781 caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac gtaaagtccg
    72841 aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa caaaaggtag
    72901 tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga attttattgg
    72961 aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca cagatggatt
    73021 tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga tgaatctatt
    73081 aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga
    73141 cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg ccgttttgtt
    73201 tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg ctgttcgcta
    73261 tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat tagtcgaaca
    73321 atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc attgaaaaaa
    73381 aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg
    73441 tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa
    73501 ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg aacatcaaag
    73561 atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt gaagatgaat
    73621 tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt ggcgcagtag
    73681 attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat aaaaagctgc
    73741 atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca tttttctgat
    73801 aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat ataattatta
    73861 ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat tctgtgtaat
    73921 gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt cgattgtccg
    73981 ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa gatgactatt
    74041 acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta gcaatcgttg
    74101 gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta cttgatccta
    74161 ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc gacctgaacg
    74221 aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa gatccgttgt
    74281 ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt tcctatcaca
    74341 caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa caagtaggga
    74401 ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc ggtatgagac
    74461 aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac
    74521 cgacgacagc cctagatgta accatccaag cacagattct tgatttactg aaagaccttc
    74581 aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc gtagcagaaa
    74641 tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag
    74701 aactgtttga tcatcctaag catccttata cacgctcttt actgaactct ataccacaag
    74761 aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca
    74821 tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca agcgatcatg
    74881 aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc tcctgctata
    74941 agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta atcacaatca
    75001 aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta acggatcatg
    75061 tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat ggtttagtgg
    75121 gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa
    75181 cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa
    75241 tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt ttgaatccta
    75301 aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc
    75361 aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg gaagatgcgc
    75421 tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga gtagcccgtg
    75481 ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct ctggatctat
    75541 ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac ggcctcagct
    75601 atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat atcgcaatta
    75661 tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat gacccgcgac
    75721 atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa
    75781 gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa aaagaatact
    75841 atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac aaagtggcgt
    75901 tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt tattgatgat
    75961 cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga tgccggggga
    76021 tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac agatgcgaca
    76081 agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg gaaatgcttt
    76141 gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact tgatcggcga
    76201 acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt atttgattgc
    76261 tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata agattgtcgt
    76321 aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga tcatgctatt
    76381 tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac taggtttgca
    76441 aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac catcaattac
    76501 ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag taatcgattc
    76561 gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga ataaagtatt
    76621 cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat
    76681 cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc cgggagtcgg
    76741 aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat ccttagtatt
    76801 gattttagga atcgcgacgc tagtggggac attactttca gatatcatca tgagtatcgt
    76861 tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc gatgagtgaa
    76921 aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat gattgctcga
    76981 gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt tttattatta
    77041 gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt tagtatcttc
    77101 gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat tctgggagca
    77161 gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa ctctgttacg
    77221 atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg gatcatctct
    77281 ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt tatcatgatt
    77341 ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata caatgtctgg
    77401 tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt attccgaagc
    77461 aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat gggaaccaat
    77521 gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat cattactaat
    77581 ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt cttaggtttc
    77641 ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag taacggagaa
    77701 gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt agttttgatg
    77761 ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg ccaacggtta
    77821 ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg gatttgcagc
    77881 gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata cctctggaaa
    77941 caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg caacaaaaaa
    78001 taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt
    78061 taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt ttatggctcc
    78121 ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg acggagctgc
    78181 aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa
    78241 atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg aaatcattgg
    78301 gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg taggtatgga
    78361 agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag acgacaaaac
    78421 agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg gtggtatctg
    78481 gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata tggaatcaag
    78541 cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta atatcgttgc
    78601 aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc ctaaattgaa
    78661 aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga actctaaatt
    78721 atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag aaattgctgg
    78781 ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca agttaggtaa
    78841 atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg ctgataaatc
    78901 attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac gtttctatgc
    78961 aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat accatgatga
    79021 tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag atgatgctgg
    79081 ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata aactaacaat
    79141 caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt actatatcca
    79201 acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga tcgaattcaa
    79261 ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc aagctgcttg
    79321 gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg cgttcaacta
    79381 cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt caagcaaagc
    79441 atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg catttgaaga
    79501 agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa
    79561 agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg ttacagcaga
    79621 ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt cggttttttg
    79681 atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat gacttctcca
    79741 ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca aaaatatgga
    79801 aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt tcacaacttc
    79861 tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca tttcaaattt
    79921 gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa
    79981 atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt tcacgatcta
    80041 aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg caatctgcaa
    80101 ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact gatggtttct
    80161 cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa aacacgagct
    80221 gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat
    80281 gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc attgctttgc
    80341 gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga cacgacccat
    80401 gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc acatgagatg
    80461 gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc aatcgaatac
    80521 cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga agtttatgta
    80581 gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga acaagctgcg
    80641 gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt atttgaaacg
    80701 aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt ttgaaaatag
    80761 cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag aggctatccg
    80821 ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat
    80881 ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag tgattttaga
    80941 caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc gccgttatcg
    81001 acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa aagatatcca
    81061 agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa
    81121 agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg aagaagcagc
    81181 tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga
    81241 agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt taactccttt
    81301 ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac tgactcaaac
    81361 cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca
    81421 agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga tccaagaaca
    81481 agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa
    81541 aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag gacaaaaaga
    81601 tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc aaacttcatt
    81661 agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct tgatgaaagc
    81721 agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa aaacacagca
    81781 agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa gagatcaata
    81841 tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca
    81901 atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg aagtagaagc
    81961 atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg cgcagctaaa
    82021 atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt
    82081 ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc aagtccagca
    82141 gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg gtttttacca
    82201 aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg gagcagtggc
    82261 tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt taggtggggc
    82321 agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca cgtatttgaa
    82381 acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac caagacaatt
    82441 accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga ttgccagtga
    82501 acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag gaacgatcct
    82561 tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg
    82621 agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag gtggagcgaa
    82681 taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac agttgactag
    82741 tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc aggagctgca
    82801 acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag gcgaacaatt
    82861 acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg aacttgaacg
    82921 ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc agtttatcga
    82981 agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg aatcgcaacg
    83041 acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag
    83101 aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg tcttgaaaga
    83161 gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag cagaggcaac
    83221 cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact tttctgatca
    83281 tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc agcgagaaac
    83341 attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa aggagatcga
    83401 taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca
    83461 atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc atttgaccta
    83521 tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc aagaaacaac
    83581 cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg aaaaactggg
    83641 accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac atacattctt
    83701 agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga
    83761 gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc gtcaagaatt
    83821 caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac tgacggaacc
    83881 aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca aaaaactgca
    83941 aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt tgctcttttc
    84001 cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag cagcgttaga
    84061 tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg acacacaatt
    84121 tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt acggagtgac
    84181 gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata tcaatgaaga
    84241 ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt agcaatcgat
    84301 ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa acaagcatta
    84361 gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc cttagctgcg
    84421 atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag catcactttc
    84481 aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa agtgatggaa
    84541 gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc actagatgtt
    84601 ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc cttgtacaat
    84661 gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac agatgaattt
    84721 gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa aatcaaagaa
    84781 ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat gcttttggaa
    84841 tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa agatcttggt
    84901 ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc gatgatccaa
    84961 tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga agcagcagat
    85021 ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa atttgtatta
    85081 gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta ctggcgaaga
    85141 gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa
    85201 aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg atgaagactt
    85261 ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa cagcgatacg
    85321 aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt
    85381 ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt
    85441 aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg gtgttaatgg
    85501 tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa
    85561 agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt tagttgtctg
    85621 gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc cagcagctgt
    85681 cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac tagttgatac
    85741 tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt
    85801 aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag atgcaaccac
    85861 tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg tgaccggtct
    85921 tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta tccgtaatga
    85981 attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact tggagccgtt
    86041 tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag aataacccat
    86101 gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa
    86161 catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt ctcaaacaat
    86221 ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg gaggtcactt
    86281 aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta tcgaccaagc
    86341 tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac
    86401 cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa cagattttag
    86461 cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat tatttcagta
    86521 tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga tgatctggcc
    86581 attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg caaaattaaa
    86641 aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga agcgaaaaca
    86701 gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa gaacattgtt
    86761 cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt atttgaaaac
    86821 agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg cttcaccaat
    86881 gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc gcagtattcg
    86941 caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa
    87001 acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg aggagaaaga
    87061 attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag caacaacagg
    87121 tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat tgggtatcaa
    87181 tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga cagaagaaaa
    87241 agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag tagcactaga
    87301 acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg aaggaatgaa
    87361 gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg aagcattgac
    87421 tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag cacagctatc
    87481 gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa gccatgaacg
    87541 tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat ttatgttcga
    87601 tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat ttttgcgtct
    87661 gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta aggacagcta
    87721 tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg
    87781 gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa aagcttatcc
    87841 gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg ggttaggtat
    87901 cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag ccattgcttt
    87961 tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat atcgtttctt
    88021 tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg ttattggtga
    88081 cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt tgcgtggaac
    88141 gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat tgatgttgaa
    88201 agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt atctaaggga
    88261 gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat tttctgaagc
    88321 ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta gtggtatcga
    88381 tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat atgggatcat
    88441 cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag gaaaaggcag
    88501 aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa agaaaaagct
    88561 tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat
    88621 ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt ttttggtttt
    88681 tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc aatcacaggt
    88741 tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt tgcagtgacg
    88801 taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat atattggttt
    88861 tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg ttttttgagt
    88921 tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt catactaaga
    88981 tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt cactaaaggt
    89041 gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat aaaaaatacc
    89101 ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata cctaaaacat
    89161 atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa aatcattctc
    89221 aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag ttattttaag
    89281 tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc tccagttaga
    89341 tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt gtttgatcct
    89401 tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc aaaataaatt
    89461 tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt tagtggctat
    89521 gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt gctttgcttt
    89581 tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa atcaattgat
    89641 gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta ctttttttgc
    89701 ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt ttgcgactgt
    89761 gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct tggtttggct
    89821 atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa atctgggaat
    89881 agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta gtgcagtcat
    89941 gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc ttgtctctag
    90001 aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag gtagtttgtt
    90061 cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa taccagtcag
    90121 tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa ggggacgacg
    90181 atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt ttatttgtta
    90241 caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt gcattgagga
    90301 gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc acaatcagct
    90361 ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt gagtcgttat
    90421 atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg cttcagcaga
    90481 caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt ctattgttat
    90541 ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg tcagggatga
    90601 ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg gatccgaatg
    90661 aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag agtcagtatc
    90721 ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga tatcgacatg
    90781 tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata gacacagatc
    90841 gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca acggctcttg
    90901 tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt gtcttttcta
    90961 cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg tttctgacag
    91021 gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt gtcgtcgttg
    91081 aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag agtatcctat
    91141 gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta aagatgtaca
    91201 gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg ctggaaagtc
    91261 aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa tctatttaga
    91321 ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat cgatcctacg
    91381 acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt
    91441 tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg aatcactgga
    91501 acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag gcggtcaact
    91561 ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt tactggatga
    91621 acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt taaaaaattt
    91681 agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact ttgctgcaag
    91741 ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag gaacgaccca
    91801 agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac ggatttgtga
    91861 gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga gaagagatga
    91921 aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca tgtggaaata
    91981 gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa actacattga
    92041 cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg
    92101 tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat aaggtagttg
    92161 gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta gaatcagcag
    92221 gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat ctaatcatca
    92281 tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg accattttcc
    92341 tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact gctttagggg
    92401 agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct agtgcaatcg
    92461 atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca cttgtgaatg
    92521 aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat gatttatttg
    92581 gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt gtttcttatg
    92641 aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca aaagctattg
    92701 gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa acgaatgctg
    92761 gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc gggggcggtt
    92821 tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag cataaataac
    92881 agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta aatagcaaga
    92941 gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg attttacgga
    93001 tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta catcatacgg
    93061 gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca gcatcaagat
    93121 cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact taaatttaga
    93181 tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc ttctcggcaa
    93241 taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc ttattactca
    93301 aagcaaatcg cagttgtcac aatctcttct aaacgatgtt ccatctgctg cttctcggca
    93361 ataagcccac tagactagaa aatatgagaa gctattttct gtccagcgtt cttattactc
    93421 aaagcaaatc gcagttgtca caatctcttc taaacaatgt tcaatcagct gaccttcggt
    93481 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
    93541 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
    93601 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
    93661 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
    93721 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
    93781 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt cttttatttg
    93841 ataagtttac tctaccgaag agttgttaat aatccgtgaa caaaaagtaa ttctttttcc
    93901 aatagttgtt aaaggttttt tattttttat agtatttttt gttatttaag aaaggaataa
    93961 ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac tgaacgaaga
    94021 aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg acttactttc
    94081 caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa taaagtgatt
    94141 attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc acaagaagta
    94201 ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa cggtgacggt
    94261 ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt gatcattgtc
    94321 aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac accagttgga
    94381 ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac acttgcttta
    94441 gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg
    94501 caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat cagcgaccta
    94561 catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca agttttgcaa
    94621 aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt agaccgttgc
    94681 ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca tttcggcaat
    94741 cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga cccacatttt
    94801 ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat gaatggctgg
    94861 tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg
    94921 tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg
    94981 cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga ggtgatttta
    95041 tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc ttatgagcgt
    95101 tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact tgaccgttat
    95161 tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga taaatccatc
    95221 ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca gctgacccga
    95281 gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt acgtgtcctc
    95341 ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca agaggaattt
    95401 gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta aaagaaaata
    95461 ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga tatgctgctt
    95521 tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt
    95581 actagatgca agcgtgtatt ctttttttga acgataatca caaaaaaaaa tataatcgta
    95641 aatttcaagt ttaagttagc cacccatggt gacccggttg ctcgttacca ccaaccgtct
    95701 aatttttcta atcgagtggg cccttgacaa tgagctgagg catggtagtc tcccgactgg
    95761 ctggccccgg ccagctttcc agacacaagc catgccgagg gaggctcgtt gtcaagggcc
    95821 cacgtgtcaa gccgtcaacc gttcctgcct aaatgcctgg acgaacgtgt catgcggcgt
    95881 tgcatagccc aaaatctttc gcgggtaatc gttcatccaa cgctgaatcc gcaagatttg
    95941 gttttcgctc acgttgccga ttgggctgcc cttggggatg aaacggcgaa tcagcttgtg
    96001 ttggttttcg ctcgtcccgc gctcccagga agcatagggg tggctgaagt agatgtttgc
    96061 cacatcctgc aaggcatcgt gcagtcccgc gaactccgag ccgttgtccg aggtaaccgt
    96121 cttgaacagc tggccgaagt acgcaccagc ccgttctctc agctgtcgga ccgcttggtt
    96181 gaccgattcg gcatccttcc cggccagctt cagaatgacc tcgaagcgtg tctgccgttc
    96241 caccaacgtc agcagcacgg catccgactt ggtcttgttt cctacgaccg tgtcgacttc
    96301 ccaatggccg aaggttttcc gggtaccaac cgcctccggg cgctcttcaa tggatttccc
    96361 tagcacccgt ttattcgggc ggttgccatg acgggacgat ttcggtttgc gggacagttt
    96421 ttctagcagg tccatattct tcgttttcat gatgccacgg tcgatccact gatagagggt
    96481 ggtggtccca gggataatgg ccgggtcgaa caattcgtgt tttctggcaa agcccaccac
    96541 cacgtccggg gaccattttt ccaacagcat catgtcgtcc gcccattcaa tgaaggcgtc
    96601 cgtgtcggcc cataagggcc gtctgccgca gttcaagcgg ttcttctcgt aggccgcctg
    96661 cccggcatcg gcgtcgtaga cgtcatgata atagtcgtag accttcccgt tctgattctg
    96721 gcggttaagc tgagtaacgg tcccgcgttt gatttcgttg tttatggtct gcggcacgca
    96781 gcccaggaca tccgcaacct gacggttgga atagttttcc tttttcagga tggcgatttg
    96841 gcaacgttct gagtaggtta ggtgttttcc cttgcgtgaa actgtgttat catggttttg
    96901 cgtcatgtga attcatcctt gtctattgag agttgtggta acttcaatat aacatggaat
    96961 tcacgtggcg tttttttatt tccaaatggc taagttaatt ataaaatccg ccttttttta
    97021 aaagtaacaa aatttcgaaa aatatgcaac aattttcaga aaattcccta tataaaacga
    97081 tggttattta ataacgtaag tgataaaata aaagcgctga atcatctgag gaggaatcat
    97141 cgtgacaaag tcgagattag aagcatttac tgatggagtg gtagccattg ttttgagagt
    97201 tattgtatta gagatcaaga tccctaatac atctagcttt cattctttat gggtcatccg
    97261 aaatacatta ttagcttata ccatcagctt tatatttgtt gcggtgatct gggtgaacca
    97321 tcatcgtata tttcagatgg cagaacgaat taattaccga gtggtctggt cgaatatctt
    97381 tttgctcttt tggctgactt tatgtccttc tgttactagt tgggtcgggc gtaaccctga
    97441 agctttttgg cctgagatgg cttatgtctt agtatacacg atgtggagtt tttcttttgg
    97501 tatcttgatg aggcaaatca tgaaagcaaa taaaccagat tcccatgtcg tgagagtttt
    97561 gtcaaaggac cgtcgaagtc ttatctccat gatgattaac ttatgcttga ttgccggtgt
    97621 cttttttgta ccgttgatcg ggttattcgg acgttttttt gtatctggta tttggatttt
    97681 ttcctataaa aaagcagatg cctattatca gcggttgttt ccggggaaat agaaaagagg
    97741 ctgggacaga agcttttatc tccaagaaat aagccgaaat ttcacaaaaa tttaagaagc
    97801 aattttcgta aattccttct tatttctcga agctgaccac ttctgtcaca actactttct
    97861 tacttcttac gttcatattt catatgatag attagtaata gaagcaagaa ccacacaggt
    97921 gttagtaata aagcgattct cgtatctttt cctaggaaca agacgacaca cacaaaggcc
    97981 atgtaaagaa ataccagata attactaaat gggtacaacg gcattttaaa tgaatgagct
    98041 tttttgccga ggatactttt acgatatttc atgtgcgtgt agacaagcat actccagata
    98101 aacaagaaac aagtagtggc aatactgctt actagtgtga atacttcact aggcatcacg
    98161 taattcaaga taacagaaat gaaaatgacg atagtagaaa atgtcaaagc attccctggc
    98221 acatgatgtg ttgttaactt tttgaatttt ttaggggcac tgccttcttg cgctaaagaa
    98281 cgaagcatac gtcctgtact atatattgca ctgttgcagg cagaagctgc tgcacttaat
    98341 acgactaaat tgatcaagga agcagcaatc gtgatcccaa tttccgaaaa tacttctaca
    98401 aatggactgt tatctggatt caagctgttc catggataga ttgccataat gacaaaaaga
    98461 gatcctaagt aaaataagat gatcctgata ggaatattat tgatagcttc cggcaacact
    98521 ttttcaggat tttctgtttc tccagcgact aatccaacta attctatccc agcaaaagca
    98581 aaggttacca tttggaaagc taagataaat ccttttgcac cagttggaaa gaatccgcca
    98641 tgagaccata gattcgtgat actggctgtg ccaatatctg tcgtatagtg ggtgaggatc
    98701 atataagctc ctacgaggat aaaggcaatg atcgctacaa ctttgatcaa tgcaaaccaa
    98761 aactctaact caccaaacaa tgagacagcc accatattca agcaaagaag aagacctaat
    98821 gcaatcagtt caggaagcca ctgaggaaca ccaggcgccc agtagcgtac atacattccg
    98881 atggctgtta aatcggccat tgcaatcgat acccagcaga accaatacgt ccagccagtg
    98941 atgaaagccg cttttttgcc aagatattct gcaacaaaat ccagaaaaga atggttattg
    99001 gtgtttgata acagaagttc gcctaacgca cgcataatga aaaaacagat gcatccagta
    99061 ataagatagg cgagcaatat cgaagggcca gctagctgga tagactttcc ggaacctaaa
    99121 aacaaccccg ttccaatcgc tccgccaata gaaatcagtt ggacatgtct atttttcagt
    99181 ccacgctgca gttcttgctc ttcattcatt tttaaatcat cctaactaaa aaagtttaat
    99241 tacttccata ttaagagagt tttctgaaaa acacaactaa gattccaaaa ttttcagaaa
    99301 tttacctttt atgagtaatt cataaaaaag aactgcggta aacaccgcag ctctttgtcc
    99361 caatcagcta ttgatcctaa gcatttaaaa tacgtgaaag gaattctttt gtacgttctt
    99421 ctttcggatg gacaaagata tcttctggtg taccttcttc tgcaatcaca cctttatcca
    99481 taaaaatgac acggtcagat acatcttttg caaattccat ctcatgcgtc acgataagca
    99541 ttgtcaagcc agtatgtgct aagtctttga tcgtattaag cacttcgcca accatttcag
    99601 ggtctaacgc cgaagtaggt tcgtcgaata gcaatacatc tggattcatt gataatgccc
    99661 gagcaattgc gacacgctgt ttctgtccac cagataattg tgctggtttg gcttcgatat
    99721 aacgatccat accaactttt tccagatttt cgattgctac ttttttggca gtttctttat
    99781 ctcgtttcag gacagttgtt tgaccagaca tacagttttc caagacattc atattgttga
    99841 acaggttgaa ggattggaaa accataccta gattcgttcg atatttaggt aaattatatt
    99901 taggagctaa tacattttct ccattataaa tgatctcgcc gcctgttggt ttttccagta
    99961 aattgataca gcgcaagaaa gtagatttac cagaaccgga agaaccgatg atcgtcacaa
   100021 cttcaccttt attgactgtt acgctgatat ctttcaatac ttcattttca ccgaagcttt
   100081 ttcttagatg tttgatttca ataatttttt cattcatgat tcttcctcct tattcctgca
   100141 aatttgaatt gtcgatttct tcaagcttgg tataagctga aggaccatcg attttctttt
   100201 ctaccgcccg caggattctg gtgatcgtaa aggtcatgac taagtagatg atcccgacga
   100261 tcgtaaatgt ttggaagaat tggaaattcg caccggaagc agagtttcct tggaagaaca
   100321 aatcagctac gccgattaca cttaaaactg ctgtatcttt gatattgata acaaactcat
   100381 ttcctgttgc tggcaagata ttccgtaaaa cttgtggaat aacaactttt ctcatcgttt
   100441 ggccatgggt cataccaatg gcttgcgcag cttcgaactg cccttcatct actgcaaaaa
   100501 tccccccacg aacgatctct gtcatgtaag ctccagtatt gagagagaca ataaacaaag
   100561 cggcaatcgt tcggttcaat gaaatgccaa aagcgagtgc cagaccgtag aagatgacca
   100621 ttgcttgtac catcatcggt gttccgcgga atacttcgac atagatagaa agaagtgcat
   100681 ttccgatttt ttggaagaag cgagtcagtg catttcctga ttcaggtacc gaacggaaga
   100741 caccaatcag taatccaagt gtagtaccga caactgtccc gatcaatgcg atgaacaagg
   100801 taagtccagc acctcgtaag aacatattgc cgtattgttt ccaaatcgta gtgaaatcag
   100861 aaatgatgcc aggtttttct tcactatcag ttgcagcagg ctgatctttg actgcttggt
   100921 ccataatttt ggttcgttca tcgttagaga tgccagaaag aatctggttt actttttgga
   100981 tatccgggtc gcctttacgc ataccaacag aaatctgaac atcttccggg tttgtctgga
   101041 agccatctgc accgctgaat tcaagcattt ccaagtcttt gttcacacta gttgccgtta
   101101 caccttccgg acgttcgctg acataaccat cgatcattcc agaagctaaa gctgttctca
   101161 ttgctgagaa attatccatt gcttgttgtt tctgcacatc tggtatctga tcgatcacag
   101221 tatagtggaa tgtatttaac tgagcagtga tctttgctcc agaaagatct gctaaacttt
   101281 ttgcttttgc atatttgcta tctttttgaa ctactactac taattgtgat tcgtaataag
   101341 gatcggtaaa atcaatttct tttcgtcgtt cagcagtagg gctcattccg gcgatgatcg
   101401 cgtcgatctt gcctgattga agggcaggag ccagaccatc ccatttcgtt tggacgatga
   101461 ctaatttttt ccctaaacca tctgctactt ttttagcgat ttgaacatca taaccgccgg
   101521 cgtaactatt ttggccttga attggcaagg cattattctc atctgtttgt tgagaccagt
   101581 tgaagggtgc atacccagcc tccataccta cgcgaaactc atcgctcggt gttttttcat
   101641 ctgcatttgc cagagatccc acactgaata gtgagaagac aaaagcaaat agtacggcaa
   101701 ctgaaaccat attttttttc atttttttct ccctttctga ttatatgata cacgagcggt
   101761 ctagatgaaa tgcggtaaag ctttatctga aaaaataaaa agcgaccgat ttatagatat
   101821 aaaaaggccg ctaaaattcc ataaggtgat aaacctctca caaaacatag cgcaacttag
   101881 catcactgct aagacagtcc gcaagctctt aacttgcgtc ccaacataca ctccaagcaa
   101941 aaaagtgcac atttcggcga tcctccctat tcctgcttct ccatgtttgc ttcactccac
   102001 agggttaatg attctcgcga cctctacctc attgattgag gcatctcgta ttcagttatt
   102061 tatacaaaaa aagtttacga tactcgagag gctttgtcaa tctataactg ctggtttttg
   102121 aaaaaatact gataaatata gaaatcttta cttttaccga aaaaacaatc aaaatgtgaa
   102181 tcatctatcc attgcaaacg atagtataat tatacacaaa aatgtatgta atacaataaa
   102241 aaaagaacta gaacagaaaa tttcctgtgt ccagttccat ctcgatcatt taagctgccg
   102301 ctttctttgg ctcacgtcct aggatcgctt gcaaaagaac aacgacgcca taaatggcta
   102361 atattagata aatcaaactt gagtattggg tgattcccca gccccatgtt cgatatacgg
   102421 ctaatgtaat ggctaaaaca attgcggcaa tcacgttgaa taaagcaaaa gcccaaagat
   102481 ttccattttc tttgcgtaca agataaaaaa cagagaaata aacgcttaac gctaaccagg
   102541 cgatacaagc cagaataacg ccccaataaa taaagaatgg taacatatcg attcacctca
   102601 cttttataca gcagtattgt aacacgtttt cattcttttg tcagtgaaca ttgtgataaa
   102661 ttgctcaaat gcccataaca cttttttatt cccatttttc tgacttttca acaataaatt
   102721 tgttcgagta tgggctataa tggaaagtga ggtgacaaaa catgaaacaa ttaacagtag
   102781 acgaagcagc aaaagagata ttagcttatc agcaaattac atttttgacc ggtgcagggg
   102841 tatccacacc ttctggagtg ccagactatc gatctttgtc aggaatctac cacgggatgg
   102901 agcagccgga atacttgctg agtcatcaag caatggtgaa tgaaccggat aaattctatc
   102961 aatttgtcaa aaagatttat catcctgaag caaaacccaa tatcattcat ttggaaatag
   103021 cacatctagc gaaagaaaaa aatgtttggg ttgtttccca aaacatcgac ggtttgcacg
   103081 caaaggctaa aagcccgcaa ctcgtcaatt ttcatgggag tctgtatcaa tgtcattgtc
   103141 gaaaatgtca acaaacggta gactggaaag aatatcttca cagcgacaaa catcaagcat
   103201 gcggaggaca aatacgccca gacatcgtgt tatatggtga agggtttcaa gatggagtca
   103261 tggagcaggc ggcttatgcc gtcagccaag cagagctgat cgtcattgtt ggaacgagtt
   103321 ttcaagttca tccgttttgt gatctggtac agtttcgcca gccgtcagcc aaaatcttag
   103381 taatcaatca aacgcctgtt tatttagcgg agccttatac gtttattcaa gaagacggag
   103441 cggttgtttt caaaaaaata caagaattag gagcagatta tgactgaatt caaaaccgaa
   103501 aaagaaaaaa tgattgcagg cgagctttat tttgccaatg atcctgagct ggttgccgac
   103561 cgtatgtttg caagaagtca aagccaaatc atcaaccaag cagaatcagc ggaactgcga
   103621 tcccaattgt tgaaagagac ttttggccgt acagggaaaa aaatctatat ggagccagtt
   103681 atcaattttg attacggata taatatattt gttggggaga atttttatgc aaatttcaat
   103741 tgtacatttt tagatgtcag cacgattgaa atcggtgata actgcatgtt cgctccgaat
   103801 gtccagcttt acacggcgac gcatccacta catccagtga aacgtaacag cggtctggag
   103861 tacgcaaaac ccattaaaat cggtaataat gtttggttag gcggaggagt tatcgtcacg
   103921 ccgggtgtca ctctagggga caacgtagta gtcggcgcag gttcggttgt gaccaaatct
   103981 tttcccgaca atgtagtgat tgcaggaaat cctgctcgta tcatcaagac agtagaggaa
   104041 gaactaacag aagagtctct tgaaacatta cgtcataaga tcgatatgat cgatcgagag
   104101 ttggttgctt tattagaaaa aagaatggat acggtaacta aaataggaca agtgaaaaaa
   104161 gaagaaaacc aagcagttta tgatgcaaaa agagagcagc aagtattgga taaagttgtt
   104221 agtctgctga aaaataaggg atataaggaa acaataacag acacgtatgt tgatttgatg
   104281 aaacactcgc gcgaatacca aaacaaaatg aaggaggaat aagtgtgatc gaaggagaga
   104341 cacggcgaaa acaaatcatg caaaccttgg caaatacaga aaaaccagtg agtgctagca
   104401 agtttgcgaa aacttttgga gtcagccgtc agatcattgt aggcgatatt gctttactac
   104461 gagctgcagg taaaagtatc gtggcaacag caagaggtta tatcctcgaa acagaagaaa
   104521 ccaatactgg gcacatcagt aaaatcgctg tccagcatgg aaaagatcaa acagaagaag
   104581 aactgagact aatcgttgaa aatggtggtg aaatcattga tgtaattgtg gaacatcccc
   104641 tttacggtga gttagcagga actttgcaca tcaaaacgcc acaagatatc gattccttta
   104701 tgagacgtta tcaaaaaagt aatgctactt tgttatccga attaacaggc ggcatccatc
   104761 tgcatacgat ccgctaccca gaaaagcata tgctgaaaaa catcaaacaa aaactagctg
   104821 aagcaggaat cctctacgaa ggataacatc gtagctgcta ctttttatcc tagaaactat
   104881 tctatcatct ctgagataaa cacccatatg gaagaaataa gaaaaaataa gaaaagatgc
   104941 gtatccaata cacagaattt tttggaattg cgatttctag atgtctatct aaatttcgat
   105001 tagtggagaa acataaaaaa gggaagtgca gcttttgagc actccccttt tttataaagc
   105061 tttcttgtag catagtatcg aaggaacctg ccaattctct actcgatcac caatacgcct
   105121 tcttttagtg caaatggatt ttgatcgttg atgtggtcat agaacatcac accgttgata
   105181 tggtcgattt catgttgaac gacgatcgat tcataattct tcaaacgaat cttgtgtttt
   105241 tcaccattca tatcataata agacacagtg attttggcat ggcgaacaac atatcctggt
   105301 acttctcgat caacagataa acagccttca ccttccccta aacaagcatc ttgaacagaa
   105361 tggctcaaaa ttttaggatt atacataaca gtacttagag aaggtccatc tgcctctggg
   105421 tcaggacttg gtacgtgaac agcaatgatt cttttagaaa tatctaattg aggagcagct
   105481 aagccaacac cgcctcgtaa gtgtagttct tcagctttga ttggatcttg gctgtttttt
   105541 aagaactcaa gcatctcttt tcctaaagag atatcttctt cgcttaatgg aagagaaact
   105601 tcttttgcaa cttcgcgtaa ggtcggattt ccttcacgaa tgatatcatc cattgtaatc
   105661 atggaatatg tacccctttc ttaaaagact gtgtttcaag tctattttcg cattatagtt
   105721 taccacaaaa atggaaaagt agtagtaaga ttgtttgaaa actacctttt cttaatgatt
   105781 aactacttga ttttcaaccc ggtttcaggt aaagtatagc tgtgtgctct aggcacatga
   105841 cctgccactt ggttaagcga atcaccaacg ttttactcag tcgcagggga ataatcgaaa
   105901 gaggtgaaag aaatggcaat ttcaaaagaa cgcaagaatg aaatcatcaa cgaatacgct
   105961 cgccatgaag gggatactgg ttctccagaa gtacaaatcg ctgtattaac tgaagaaatc
   106021 aaccacttga atgaacatgc acgcgtgcac aaaaaagacc atcattcata ccgcggccta
   106081 atgaaaaaag ttggtcaccg ccgtaacttg ttagcttact tacgtaaaac tgacgttcaa
   106141 cgttaccgcg aattgatcaa acgtttaggc ttgcgtcgtt aattttagtc aaaaagagtg
   106201 ggattcacat gaatctcact ttttttactc gtatatcaat taagtaaaat tccgtgatca
   106261 agctagatgg actcctacta accaaatgaa agctgctcaa aagacggctt tcggcagttt
   106321 tcatttgttt agtagcgtct tgctttcacg agaaggaaaa ggagaagtta tgtcagaaaa
   106381 acaagtattc aaaacgacat ggggcggccg tcccttacaa atcgaagtcg gtcagctagc
   106441 aaaacaagca aacggagccg ttttagtccg ttatggagat acagtagtct taagtgcagc
   106501 tgtggcctct aaagaagcaa aagatacaga ctttttccca ttaacgatca attatgaaga
   106561 aaaaatgtat gcagcaggga aaatccctgg aggtttcatc aaacgagaag gccgtccaag
   106621 tacggaagca acattgactg cccgtttgat cgaccgtcca attcgcccga tgtttgcaga
   106681 aggcttccgt aatgaagtcc aagtaacgaa catcgtgatg agtgtagaaa cagactgctc
   106741 gccagctatg gcagcaatgc taggttcttc tttagcacta tcaatctcag atatcccatt
   106801 tgacggccca atcgcaggcg tagaagttgg tcgtgtaaat ggcgagtacg tcttgaatcc
   106861 aacagtagaa caagcagaac aaacagatat cgaattgact gttgccggaa cgaaacaagc
   106921 catcaatatg gttgaaagcg gcgcgaaaga agtttcagaa gaagacatgc ttggtgcatt
   106981 gctgtttggt tttgacgcta tcaaagagtt agttgctttc caagaagaaa tcgttcaagc
   107041 agtagggaaa gaaaaaatgg aagtgacttt actgcaagtt gatgaagtat tgaaaaaaga
   107101 aatctttgat gcttcttacg ctacaatgaa agcagcagta atgactgaag aaaaacttgc
   107161 tcgcgaagat aatatcgagc aggtgaagat cgatatccgc gaagcttatg ctgagaaatt
   107221 tgcaggtcat gaagatgaag atcacttgtt gaaagaagtt aaacaaatca cagaagatct
   107281 tgaaaaagat gtcgtacgtg aattgatcac catcgataag atccgtcctg acgggcgtaa
   107341 attggatgaa atccgtccat tgtcatcaga ggtcagctta ttgccacgtg tacatggatc
   107401 aggtttgttc acacgaggac aaacacaagc tttatcagct tgtacactcg cacctctagg
   107461 agagcaccaa atcattgatg gtttaggggt ggaagtcagc aaacgattca tccaccacta
   107521 caatttccct caattttctg ttggttctac aggacgtgca ggctctccag ggcgtcggga
   107581 aatcggacat ggcgcattag gtgaacgtgc gttagcacaa gtaatccctt cagaagaaga
   107641 atttccttat acgatccgtt tagtagcaga agtattggaa tcaaatggtt cttcttccca
   107701 agcaagtatc tgtgctggca cattagcatt gatggatgca ggtgtaccaa tcaaagcgcc
   107761 agtcgccggt attgcgatgg gacttgtttc tgatggtgaa aactatacga tcctaactga
   107821 tatccaaggg ctggaagatc atcttggcga tatggacttt aaagttgccg gtacaaaaga
   107881 tggtatcact gctttacaga tggatatcaa gattcaagga atcacagaac aaatcctaac
   107941 tgaagcatta actcaagcaa aacaagcacg tatggaaatc ttagaagaat tgacttcaac
   108001 gattgctgct ccacgtgaag aattaagcca atatgcacca aaaatcgaaa tgatccagat
   108061 cgaacctgct aaaatcaaag atgttatcgg taaaggcggc gatacgatca acggaatcat
   108121 cgatgaaact ggtgtgaaaa tcgatatcga tcaagatgga aaagtaagta ttgcttcagc
   108181 tgatgcagaa atgatcaaaa aagctatcaa gatcatcgaa gatttgacca aagaagtcaa
   108241 agttggtgaa gtttatctag ggaaagttgt ccgtatcgaa aaattcggtg cattcgtcaa
   108301 cttgatcaaa ggaaaagatg gacttgtcca tatttcccaa ttagcaaacg atcgtgtcaa
   108361 taaagtcgaa gatgttgtta aactaggtga tgagatcctt gtgaaagtaa ctgaaatcga
   108421 taaacaagga cgcgtaaatc tgtcaagaaa agcaatgttg aacgaagatg gttcaacgaa
   108481 aaatgaagaa aaataattta attcatagga ataatgtact tgtgatcgtg aaggtgacag
   108541 ggggacaata attattcgaa aaaaggggac tgtgacaaaa gccgtgtcac agtccttttt
   108601 tttcgaataa acggtgttca aaagcttgca tctgcggtaa taagtccagc caagttaaaa
   108661 acattaggag ctgtttttat ttggctgttc ttattacttg atgcagaaca gcttttttac
   108721 aacctctcca ataaacgatg ttcaatcagc tgtccctcga tattagctca aaaaacggca
   108781 aaatatgagg aactattttt ccgtttgttt ctctaatact caggtcaaac cgctgatttc
   108841 acaacctctg attcacggtg ttccagaagc agcttctacg gaaataagct gaacactttt
   108901 gttacaactt ctttttttct ggagtttctt ggcgcatatt tttggagtcc ttctgaataa
   108961 agcgtaaaat aaaagtgaga aatatggata aaggagggaa aggagatgct gaatttttta
   109021 tggtcgttat ttatcggcgg tatattaggt ttgatagctg gcgcaatttt aggaaaggaa
   109081 gtcccgggag gcgtatctgg aaatatcatt atcggttttt taggtagttg gctcggcgaa
   109141 tttttactag gaccactggg accggaactc ggcggttttt atttgatacc agcattattg
   109201 ggtgcaattt tttgccttgc tctttattca tttgtggcaa gacgtatcag agaacgtcca
   109261 aagaaagaat aattttattc aaataagttg acacttcagt gtgttacttg gataattaga
   109321 agcaatttag gaaagcgggt gtcatggata agaatatcat ctatcattca agcttaccta
   109381 aaggatatcc atataacaag aaatgaggga aaaacgtggc aacaacgcac aataccaaaa
   109441 agatatcaat gactggcctg cttgtagcga tgggagtcgt ctacggagac atcgggacaa
   109501 gtccgcttta cgttatgaaa tcgattgttg aagataatgg cggattgtca ggaatcaatc
   109561 ctgattttgt tatcggatca gtctcattga ttttttggac attgactcta ctgacaacaa
   109621 tcaaatatgt cgtgattgca ttgaatgcag ataaccatgg tgaaggtgga attttctcac
   109681 tctacacact tgtccgaaaa ggtgggaaat atttgatcat tcctgcaatg atcggaggag
   109741 ctgccttact agcagatggg gtattgaccc ctgctgtgac ggtaacgact gcgattgaag
   109801 gcttgcgagg aattccgcag ttttttgacc ggttcggtaa taaccagaac gtgatcgtca
   109861 ttatcacttt agttattatt tttgttttat tcatggtcca acgctttggt acagatattg
   109921 tcggaaaagc attcggacca atcatgttcg cttggtttac ttttttaggc gtaatgggat
   109981 taatgaactt cagtcaagac tggacagtta tccgtgcact gaatccttat tatgctatcc
   110041 agctgctttt tagtgcagat aacaaattag gcttgttcat tcttggaaat attttcctgg
   110101 ctacgacagg tgcagaggcg ctttattccg atcttggaca tgtcggcaaa cgaaatattc
   110161 gtctgagctg gccttacgtc aaaatttgtt tggttatcaa ttattttggg caggctgcgt
   110221 ggttgttgaa tgtttatcaa aaccctgaag cacagcaaat caagaacctg aatccattct
   110281 tccaaatgat gcctcaaagt tggatcgtga tcggcgttgc ttttgcaaca gtcgctgcag
   110341 tcattgcttc gcaagcattg attacaggtt cgtttacgct agtttcagaa gcaatcaagt
   110401 tgaaactgct gcctcggtta aagatcatgt atcctgggaa cagtatcggg caaatgtata
   110461 tcccagcagt caatctgatt ttatggatcg cctgttctct agtggttgtc acattccgaa
   110521 cgtctcatca tatggaagct gcttacggtc tatcgatcac tgtgacgatg ttgatgacaa
   110581 cagcactgct ttacttttat cttatccaaa atgggcattc tcgttggctt gcatatttag
   110641 ttaccttttt cttcggtgcg atagaagtcg ttttctttat ctcaagtgtt gtcaaattct
   110701 tccgaggcgg ttttgtagcc gttctgattg gtttgatcat cttagtcgtg atgtttattt
   110761 gggaacaagg gaacattatt cgcgaatcgt caaccgagga tgtgtctttg agagattata
   110821 tcccgcagct tagagaatta cgtgatgata cttctttacc aatctatcag acaaatgtgg
   110881 tcttcttggt tcctgatatg gctgaagata aagttggccg acagttcatc tattcgattt
   110941 tagataaacg gcctaaaaga gcacgagtct attggtttgt tcatgtggaa gtgacggatg
   111001 agccatatac gaaagaatat caagtagata tgatggacac tgattttatt gtccaagtga
   111061 atctgttctt aggtttcagg gtccaacagg aaatcaacgt atatgttcgc caagtcatcc
   111121 atgatttgat gaaacaaggc cgtttgccaa aacagccaca aagatactct ctgacaccag
   111181 ggcgagaagt tggcgatttc caatttgtcc tgattgatga agtagtttcc aatgtgacaa
   111241 ctctaggaaa atggcaacgt caaatcatgc aggcaaaact ggcaatcaaa aaaatcgcta
   111301 cctcgccaga aacatggttt gggctagagt acagcgaagt aaaacatgaa actgttccgc
   111361 ttatcattgg aaatactcgg aaaacatggt tgaaagagcg gttgtgaaag tagcgctttg
   111421 acctgaacta atgtcgaatg tcagctactt gaacaccgtt tatcagaggt catgaaaaag
   111481 ctgtactgca acaagcagta agaacgagca gataaaaaca gcttctcatg tttttcatct
   111541 gtttgggctt aatgccgtag atgcaagctt ttgaataccg tttttatcag aggtcatgaa
   111601 aaagctgttt agcaacaagt aacgcaatga ttttgaagtg ataaggagat gtggctgaaa
   111661 aatgccgcat ctcctttttt attcctaaat atgaatacgc attattttca atctttttgt
   111721 agaaaaaggg tacaatagaa aaagagaaag gcgggagaat atggttatca atttcattat
   111781 tttatttttg atgattgcaa ttacttcatt ttttgtagca agtgaatttg ctttagtgaa
   111841 aatcagacga tcaagattgg aacaactaga aaaggaaaat gtaaagaacg ccaagctagc
   111901 tcttcatgtt acacaccatt tagataacta tttatcagct agtcaattag gaattacgct
   111961 aaccggtttg atcatcgggt gggtcggtga aggatctgtc gcggctttac tagaaccagt
   112021 gatcggtaaa ctacctttta gttcggctat tagcagtaca atctctgtgg cgctgggatt
   112081 tattttagtt acgtatatcg atgtggttat tggcgaactg cttcctaaga gctatagtat
   112141 cgtcaatacg gagaaggtcg tcttgttcgt agttaaacca cttcattatt tttataaagt
   112201 aatgtttcct tttatttggg tattgaatca ttcagctgca gggctaggga agctattagg
   112261 tgtgcgtctc gtatctgaag gagaagagac attgtctcaa gaagagctga cattagtcgc
   112321 attgaattct tatgaaaaag gagagttgac caaagaagaa tattattatt tggaaaacgt
   112381 gtttgaattt gatgatacct tggcaaaaga tatacaagta gaccgtacgt ccatgcaagt
   112441 attcgaagct tcagaaaccg tcaaagaagc gataaaagct tcgattgaac aaggacatac
   112501 tcgctatccg gttatcttag aatcaaaaga caatgtttta ggttatgtca ccttgccaga
   112561 tttgatcaag caatcttata aagatgacca gctgacagta gaacagttga ttgaagagcc
   112621 gatcgtaaca actgaaacga tccctataaa aaaattattg acgatcatgc gaaaaaaggg
   112681 taagcatatt gctatcttaa aagatgaata cggagggaca agcggcttag tgacgattga
   112741 agatatttta gaagaaatcg tcggggaaat ccgagatgag acagatttgg acgaagcatt
   112801 gatagcggaa cagtcagatg gctcttatat catctccggc aaactaacat tagatgattt
   112861 ccagcgttat tttcatgtag agattccaga gtttgaagaa acgaatttta ctacacttgc
   112921 gggctttgct tctagccgat ataaagaaat aaaagcagga acaatcattg agattgcatc
   112981 attccgtttc acggtattag aataccagca tgcacatatc gattatttca aagtagagtc
   113041 cacggaaaga aaaacagaat aaaagaaaaa aggctgtgac attttttgtc acagcctttt
   113101 tgttatctaa aaaacctgtt ttcggtaaac gaagaagata ttttcaataa aagttttcat
   113161 ttttagccgc aacgatttct tttaatcaag gctatcttgc ttctgcatgt tctaatgtga
   113221 aatgttcctc ttcacccatg tgtttcaatt gtttcatcga acgcatataa gagtaaagca
   113281 acacggcaac agaaccgatc caagctaaag gagaagctgc tgctgctcct gggtatcccc
   113341 acattcctgt aagaacgata gctgcaaaag aacgcataag caattccatc ataccggcga
   113401 tcgtagggat cttgctttgt ccaagccctt gaagtgtgta gcgtaaaatg aacaggactg
   113461 ctaagatcca gtaaagagac ccattgatat tgaagtaggt ttgtgctaat tcaaatacac
   113521 gtgtttctga tgggctaaca aacaattgaa caaagaaatg accgaagaaa atgacgatag
   113581 cacctgctaa gagactaaat ccgacactca tcagtaaagt ttgacgtacc ccttgcaaga
   113641 ttcggccata ttctttcgca ccatagtttt gtgcagtgaa agttgccatt gcaataccaa
   113701 atgacatcat cggctgattt gcaaattggt cgatacgacc agcagcggct tgagcagcta
   113761 cgacatctgt ccctagacta tttagtgctg cttgtaacac gatagcacca acagcgatga
   113821 tcgaagattg aaacgccatt ggcaaagcag catttaggtg tacagcaaac tcttcttttt
   113881 caaatttgaa atcttttttg cgtaactgta acaaaggcat ttttttcttg atatacacta
   113941 gacacagtac gctggaagaa acttgtgcga ttactgtagc aatagctgca ccttccacac
   114001 ccatgccaaa atagataata aagatcaaat caagtacgac gttgatgatg accgcaatga
   114061 tcagaaagaa tagtggtgtg cggctgtcac caagtgcgcg aatgacatta gaaagcaaat
   114121 tgaaactgac tgatgcaaag atccctaata aaataattga aataaatgtt tgtgcttgat
   114181 caagtatttc aggaggagtt tgcatcaatt gaagcatcgg acgaataaac aggagactta
   114241 ggacagttaa aatcacagtg acgacaagac taatgacaac actagcagca aaacttttct
   114301 tcagaccccg ataatctttt gcaccgtaac gttgtgcggt aataatggct aaaccagctg
   114361 ttaaaccttg ggcaaagcca atgatcaaaa atgtcaggct tccagttgca ccaaccgctg
   114421 ccagcgcatt tttacctagc gtctgaccca cgatgatcat atcgatcata ttgtagaact
   114481 gctgaaaaac atttccgatc agtaaaggaa cagtgaacat caaaatcagt ttagcggggc
   114541 ttccttttgt taaatcgcgc atcttttaaa ctccttatat ctttaaaatt tagttagtag
   114601 tatatagcgt ttatcctgag aataccaatt agaaatcaat gataatttat gaaactccat
   114661 aaaaagaagc caagaattga gtcaaagaat caacaacttg gcctggtctg taaaataagg
   114721 gacatccacg tttatctatc accttaatat tgtctaatct cacttggtaa aaggatcgtt
   114781 tctctcgtac cgtcatcata ggcgtatact tgagcaggaa attggttcga ataggcaaac
   114841 ggcaaatcaa tatccatttc cacattgctt cctgcttgag aaaaatgcat cgtcactttt
   114901 ccacgatttt cgatcaaatc aaaggttaat atattttcta atggaatgac tcccttaaga
   114961 tctaggtcaa taataaacca tatactgtca atcaattcat ctggcaaact tgatacgaca
   115021 ccaactgaag cgtaacggtt gcggctgcta tcaaaacttt caaacatctg tttaacctcc
   115081 ttcgaatcaa cgaatgtttt atttttataa ttaatcctag gataaactag aaatacttac
   115141 ttcaacgagg gttcattcta ataaagaatc aggaaaaatg caacaataat tgaatacttt
   115201 ttttcaaagt ataaattttt ataaaggtac gataaaagta tgcgaggaaa agcttggtaa
   115261 attaggattt ttcgtttgta aagccattta caaaagtgga agccccatat gaaaataaac
   115321 gattagaaat aaaaaaggcg tgttaaaagc tgctgacccg ccgcttctaa caccacctga
   115381 ttgtttcagt attattcttc tgttgaaccg atcacttctg gttcgccacc ttcacctgtg
   115441 ctttcttctt cttcactttg agcgactaca gcagcgattt gctcttcact gtcggtcacg
   115501 aatttgtaat cgtcgttttt tggaagatcg gctactgtaa tagaatcacc taattccaag
   115561 tttgtcacgt caacttcaat tcgttcaggc aatttgtctg gtgtagcaga tacgactact
   115621 gtatacaagt tttgagctaa cactccacca gatttagcac ccactgattc gccaacaaga
   115681 acgacttctg tttcgacttc tgtttcttcc gacatgttta cagatagaaa ttctgcatgt
   115741 tcgaaatgat tggtaaaagt gttcgtttgg atcttgtgaa ccaatgtatt gactttttgt
   115801 ccatcgatgt ccatcgtgat gactgtgttc gctccatgtt cacgcaacaa tttgcttagt
   115861 gtcaaaccat cgaatgagat aggggtactt tctactttat acccataaac aacagctgga
   115921 actttccctt cgtgacgaag tttgtttctt aatgaacggg gacgtacttc tcttttactt
   115981 acttctaatg atactgacat aaccaaaatt cctccttaaa atgtgactgt ctgctttggt
   116041 aacggacaga ttttatttaa gtgaattcta aaggctaatc tcgtgcacat catcgaaggc
   116101 agggcgctta aaaaagcgca aaaggagaga tgcgcgcatc tccccttaga aagtcaggat
   116161 tcaacttttc tccaccaggt cattacgctg tgtatgagaa atccaattca ctttactatt
   116221 taaccgtaac gtgaactgtt aattaagtca aggaataact atgatgatgt atttgcaagc
   116281 aagaatattc aaggtataat taattagtag taataagaaa ggaaacagca tgcgtttaga
   116341 taaattaata gaagaaaaat tagaaacgac acgcaaggaa atgaagcggc tgtttgcttt
   116401 gaagcaagtg atgatagatg gcacagtcga atttcgacaa aatcgaaatg tagatagttt
   116461 actgcatcaa atcgaagtag caggtgaaca cttagaaaca aatgagtcct attatcttct
   116521 caataaacct gaaaaagctg ttacagctgt atcagatcca gaaaagacga cagtaatcga
   116581 tctgattgca agtgccgatc gtttccaacc attatatcca gtgggaagac tagatagaga
   116641 tacaacagga ctattgctga tcacttcgaa tggacaattg ggatatgaaa tgctacttcc
   116701 agaaaaaaag gtttcaaaga cttatcaagt agttgtgaac gaaagggtga caccggcaga
   116761 cgtaacagca tttgaaaatg gaatcgtatt cgctgatggg tatcaatgta agccggctga
   116821 tttaaaaata ctcaaagcag acaagcacca aagtacggtt ttattgacga tccaagaagg
   116881 aaaattccat caagtcaaaa agatgttctt agcccaagga aagaaagtga ccgcattgaa
   116941 acggctaagc atgggaccat tggaattaga tgaacagcta ggagtaggcg agtatcgatc
   117001 gttgacatta gcagagatga aaaagttaag tatttatttt aaataggagg tcaaaaagat
   117061 gaaatataaa actttattgt ttgatgtaga tgatacattg ttagattttc aattaactga
   117121 agccaatgct ttaagtgctt tgtttgcaga acaaggaatc acattgacac cagaaatcga
   117181 aaccagctat aaggctttga atcatcattt atggcaggag tttgaaaaag gcagactatc
   117241 acgagaagaa ataataggca gtcgttttgg gttacttttc cagcaatttg ggaaaacagt
   117301 agatagtgat caaatggaaa aacgctaccg ccactacttg aaccaaggtc atgatcttgt
   117361 tccaggaagt ctagaaatcc tgaaaaaggt ccatccgcat gctgatcttt atattgtgac
   117421 gaacggtgtg tcgcacacgc aacatcggcg attaacagat gcaggaatgc ttgattactt
   117481 caaggatatc tttgtctcag aagctgtcgg agcgcaaaag ccgatgaaag aattttttga
   117541 tcatgtcttt tcccacattc ctaattttga taaaaagcaa actgtgatca ttggtgattc
   117601 tttgacttct gatatcaaag gaggaaatat ggcagggatc gatacgattt ggttcaatag
   117661 aaataaatta ccagaaatcg aagaaatcca gcctacttat cgaatcgatt cattagagga
   117721 actttattca ttattggaaa ttgaaaagga ttaattttca gaaaaatata gcattttcgt
   117781 gttttttcgc tataatagat tagaatgaaa aaacggatac gaaagaggtt ttagtcaatg
   117841 acaaaaagca agccaattat cattggtgtg acgggtggat ccggaagcgg aaagacaagt
   117901 gtcagccgtg cgattttcaa caacttcccg gaccattcca tcatgatgct tgaacaagat
   117961 tcttattaca aggatcagag ccatttgagt tttgaagaac gattgaacac aaattacgat
   118021 cacccatttg cctttgataa tgatctgctg attcagcatg taggcgactt actgaactat
   118081 aaagccattg aaaaacccgt gtatgactat gtagcgcata caagaagtca ggcaacgatc
   118141 attcaggaac caaaagaagt gatcattcta gaaggaatcc tgattttaga agacgaacgt
   118201 ctgcgtgact tgatggatat caaagtatac gtagatacag atgatgacat tcgtatcatt
   118261 cgtcgtatca aacgggatat ggaagaacgg ggacgcacat tggactccgt gatcgaacaa
   118321 tatttgacag ttgttaaacc gatgtatcat caatttatcg aaccaacgaa gcgctatgcg
   118381 gatatcattg ttccagaagg cggagaaaac cacgttgcta ttgatttgat tacaacaaaa
   118441 gtagctagtt tcttaaacca taaatagtgc gcagcgaaac agttgaacgg actgcgaccg
   118501 tttgaaaagc aaggattctg ctttttgaac agtcaccagc caactgtttt tttatttata
   118561 acaagaggtt gtgaaaacag catcttgacc taagtattaa aaagaagaaa cggaaaatag
   118621 ctgtttatat tttaccgttt tttgagctaa taccgaaggt cagctgattg agcaccgtga
   118681 agtagaggtt gtaaaaaagc tgtctttgca tcaagcgata agagcatcca aataaaacag
   118741 ctcctcacgt ttttcgttgg gttgggctta ttatcgtaga tgcaagcttt tgaacaccgt
   118801 ttaacgagag gttgtaacag aagtggtcag atgcaataaa taagaagaaa cttacgaaaa
   118861 ttatttctcg aatttgtgtg agatttcgac ttattttttt gaagaagctc ttttgtcgca
   118921 ccccc
//
LOCUS       DACDSR010000002        94265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDSR010000002 DACDSR010000000
VERSION     DACDSR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.002"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK15_000129"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK15_000129"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9545762.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK15_000130"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK15_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9545763.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK15_000131"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK15_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9545764.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK15_000132"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK15_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9545765.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK15_000133"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK15_000133"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9545766.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK15_000134"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK15_000134"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9545767.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWK15_000135"
     CDS             complement(8053..8523)
                     /locus_tag="IWK15_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9545768.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWK15_000136"
     CDS             complement(8569..8994)
                     /locus_tag="IWK15_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9545769.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWK15_000137"
     CDS             complement(9061..9330)
                     /locus_tag="IWK15_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9545770.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWK15_000138"
     CDS             complement(9341..10936)
                     /locus_tag="IWK15_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9545771.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK15_000139"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK15_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9545772.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWK15_000140"
     CDS             complement(14489..15049)
                     /locus_tag="IWK15_000140"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9545773.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWK15_000141"
     CDS             15402..16376
                     /locus_tag="IWK15_000141"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9545774.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWK15_000142"
     CDS             16546..17946
                     /locus_tag="IWK15_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9545775.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWK15_000143"
     CDS             complement(18024..18746)
                     /locus_tag="IWK15_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9545776.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWK15_000144"
     CDS             complement(18825..19331)
                     /locus_tag="IWK15_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9545777.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWK15_000145"
     CDS             19454..21253
                     /locus_tag="IWK15_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9545778.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWK15_000146"
     CDS             complement(21321..22895)
                     /locus_tag="IWK15_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545779.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWK15_000147"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWK15_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9545780.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWK15_000148"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWK15_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9545781.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IWK15_000149"
     CDS             complement(24672..25310)
                     /locus_tag="IWK15_000149"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9545782.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IWK15_000150"
     CDS             complement(25311..25961)
                     /locus_tag="IWK15_000150"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9545783.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWK15_000151"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWK15_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9545784.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWK15_000152"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWK15_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9545785.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IWK15_000153"
     CDS             complement(29104..29844)
                     /locus_tag="IWK15_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9545786.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWK15_000154"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWK15_000154"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9545787.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IWK15_000155"
     CDS             complement(31215..32153)
                     /locus_tag="IWK15_000155"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9545788.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWK15_000156"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWK15_000156"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9545789.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWK15_000157"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWK15_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9545790.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IWK15_000158"
     CDS             complement(35313..35618)
                     /locus_tag="IWK15_000158"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9545791.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWK15_000159"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWK15_000159"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9545792.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IWK15_000160"
     CDS             complement(36724..37008)
                     /locus_tag="IWK15_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545793.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IWK15_000161"
     CDS             complement(37026..37916)
                     /locus_tag="IWK15_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9545794.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IWK15_000162"
     CDS             complement(37936..38154)
                     /locus_tag="IWK15_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545795.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IWK15_000163"
     CDS             complement(38124..39824)
                     /locus_tag="IWK15_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9545796.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IWK15_000164"
     CDS             40091..40864
                     /locus_tag="IWK15_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545797.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWK15_000165"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWK15_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9545798.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IWK15_000166"
     CDS             complement(41688..42659)
                     /locus_tag="IWK15_000166"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9545799.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWK15_000167"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWK15_000167"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9545800.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWK15_000168"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWK15_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9545801.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IWK15_000169"
     CDS             complement(45614..46438)
                     /locus_tag="IWK15_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9545802.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IWK15_000170"
     CDS             complement(46411..47091)
                     /locus_tag="IWK15_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9545803.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IWK15_000171"
     CDS             complement(47114..48052)
                     /locus_tag="IWK15_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9545804.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IWK15_000172"
     CDS             complement(48297..49148)
                     /locus_tag="IWK15_000172"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9545805.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWK15_000173"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWK15_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9545806.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IWK15_000174"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IWK15_000174"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9545807.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWK15_000175"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWK15_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9545808.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWK15_000176"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWK15_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9545809.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IWK15_000177"
     CDS             complement(56026..57267)
                     /locus_tag="IWK15_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9545810.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWK15_000178"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWK15_000178"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9545811.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IWK15_000179"
     CDS             complement(58294..59178)
                     /locus_tag="IWK15_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9545812.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IWK15_000180"
     CDS             complement(59956..61242)
                     /locus_tag="IWK15_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9545813.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IWK15_000181"
     CDS             complement(61235..62503)
                     /locus_tag="IWK15_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9545814.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IWK15_000182"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IWK15_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9545815.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="IWK15_000183"
     CDS             complement(63276..63722)
                     /locus_tag="IWK15_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9545816.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="IWK15_000184"
     CDS             complement(63815..64060)
                     /locus_tag="IWK15_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545817.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64176..64754)
                     /locus_tag="IWK15_000185"
     CDS             complement(64176..64754)
                     /locus_tag="IWK15_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9545818.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64831..65361)
                     /locus_tag="IWK15_000186"
     CDS             complement(64831..65361)
                     /locus_tag="IWK15_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9545819.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65365..66225)
                     /gene="rlmB"
                     /locus_tag="IWK15_000187"
     CDS             complement(65365..66225)
                     /gene="rlmB"
                     /locus_tag="IWK15_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9545820.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66218..66613)
                     /locus_tag="IWK15_000188"
     CDS             complement(66218..66613)
                     /locus_tag="IWK15_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9545821.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66610..68022)
                     /locus_tag="IWK15_000189"
     CDS             complement(66610..68022)
                     /locus_tag="IWK15_000189"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9545822.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68019..68555)
                     /gene="cysE"
                     /locus_tag="IWK15_000190"
     CDS             complement(68019..68555)
                     /gene="cysE"
                     /locus_tag="IWK15_000190"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9545823.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68877..70337)
                     /locus_tag="IWK15_000191"
     CDS             complement(68877..70337)
                     /locus_tag="IWK15_000191"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9545824.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70507..71664)
                     /locus_tag="IWK15_000192"
     CDS             complement(70507..71664)
                     /locus_tag="IWK15_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9545825.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71698..73068)
                     /gene="radA"
                     /locus_tag="IWK15_000193"
     CDS             complement(71698..73068)
                     /gene="radA"
                     /locus_tag="IWK15_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9545826.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73131..73658)
                     /locus_tag="IWK15_000194"
     CDS             complement(73131..73658)
                     /locus_tag="IWK15_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9545827.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73888..74694
                     /gene="proB"
                     /locus_tag="IWK15_000195"
     CDS             73888..74694
                     /gene="proB"
                     /locus_tag="IWK15_000195"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9545828.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74691..75938
                     /locus_tag="IWK15_000196"
     CDS             74691..75938
                     /locus_tag="IWK15_000196"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9545829.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76014..76358
                     /locus_tag="IWK15_000197"
     CDS             76014..76358
                     /locus_tag="IWK15_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545830.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76475..76801)
                     /locus_tag="IWK15_000198"
     CDS             complement(76475..76801)
                     /locus_tag="IWK15_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545831.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77416..78618)
                     /locus_tag="IWK15_000199"
     CDS             complement(77416..78618)
                     /locus_tag="IWK15_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9545832.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78735..79250
                     /locus_tag="IWK15_000200"
     CDS             78735..79250
                     /locus_tag="IWK15_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9545833.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79449..79895)
                     /locus_tag="IWK15_000201"
     CDS             complement(79449..79895)
                     /locus_tag="IWK15_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9545834.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79942..81420)
                     /gene="msr"
                     /locus_tag="IWK15_000202"
     CDS             complement(79942..81420)
                     /gene="msr"
                     /locus_tag="IWK15_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9545835.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81774..82403)
                     /locus_tag="IWK15_000203"
     CDS             complement(81774..82403)
                     /locus_tag="IWK15_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9545836.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82387..82851)
                     /locus_tag="IWK15_000204"
     CDS             complement(82387..82851)
                     /locus_tag="IWK15_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9545837.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83338..84606)
                     /locus_tag="IWK15_000205"
     CDS             complement(83338..84606)
                     /locus_tag="IWK15_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9545838.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84837..85574)
                     /locus_tag="IWK15_000206"
     CDS             complement(84837..85574)
                     /locus_tag="IWK15_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9545839.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85641..86210)
                     /locus_tag="IWK15_000207"
     CDS             complement(85641..86210)
                     /locus_tag="IWK15_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9545840.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86338..87210
                     /locus_tag="IWK15_000208"
     CDS             86338..87210
                     /locus_tag="IWK15_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9545841.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87552..88424
                     /locus_tag="IWK15_000209"
     CDS             87552..88424
                     /locus_tag="IWK15_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9545842.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88421..90379
                     /locus_tag="IWK15_000210"
     CDS             88421..90379
                     /locus_tag="IWK15_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9545843.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90711..91562)
                     /locus_tag="IWK15_000211"
     CDS             complement(90711..91562)
                     /locus_tag="IWK15_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9545844.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91685..92152
                     /locus_tag="IWK15_000212"
     CDS             91685..92152
                     /locus_tag="IWK15_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9545845.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92415..92897)
                     /locus_tag="IWK15_000213"
     CDS             complement(92415..92897)
                     /locus_tag="IWK15_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9545846.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92997..93860)
                     /locus_tag="IWK15_000214"
     CDS             complement(92997..93860)
                     /locus_tag="IWK15_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9545847.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        26338 a        20305 c        16934 g        30688 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaag aaatgcaata
    64141 aaaataaata agaagctcat taagattttt ctaaattatt cctcattata aaatttcaag
    64201 tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt
    64261 gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta
    64321 gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc
    64381 tgaattgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct
    64441 attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc
    64501 ctcaacagag aatagatttg attgcttata acgcttttat aataacttcc aaagctaaca
    64561 cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc
    64621 caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga
    64681 aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat
    64741 aactcctcag acataaaata attcctccat aaattcgttt atggaggaat cttatcatca
    64801 atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa
    64861 tttttgggac aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat
    64921 aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata
    64981 taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa
    65041 atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc
    65101 gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt
    65161 ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc
    65221 ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt
    65281 gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac
    65341 aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat
    65401 aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt
    65461 cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat
    65521 gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt
    65581 gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac
    65641 gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat
    65701 tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg
    65761 taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc
    65821 ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt
    65881 aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt
    65941 aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt
    66001 ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc
    66061 tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga
    66121 aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc
    66181 aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt
    66241 tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat
    66301 agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg
    66361 cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca
    66421 aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat
    66481 tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac
    66541 gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg
    66601 tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat
    66661 ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct
    66721 tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt
    66781 aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac
    66841 agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac
    66901 cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc
    66961 taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc
    67021 ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc
    67081 tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa
    67141 acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag
    67201 agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta
    67261 attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg
    67321 aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc
    67381 cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga
    67441 aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg
    67501 ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact
    67561 taatttccca taattttcaa atttacgggt acggtaatac acatcacctg aagattcata
    67621 agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat
    67681 gactcttgga tgcagtgtag caggttcgac attcaacgct ttcgtatctt cttcaaatgc
    67741 tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat
    67801 gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc
    67861 aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata
    67921 attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa
    67981 ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct cctcactttt
    68041 tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca
    68101 tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct
    68161 aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc
    68221 gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt
    68281 tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc
    68341 gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc
    68401 gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca
    68461 tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc
    68521 tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat
    68581 tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta
    68641 tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag
    68701 ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt
    68761 ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca
    68821 ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag
    68881 ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca
    68941 ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt
    69001 tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca
    69061 tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt
    69121 tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc
    69181 gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc
    69241 ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt
    69301 agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg
    69361 tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga
    69421 tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag
    69481 cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt
    69541 tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg
    69601 atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc
    69661 agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc
    69721 aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttggatg
    69781 acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta
    69841 aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct
    69901 tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt
    69961 tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat
    70021 ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt
    70081 tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt
    70141 cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg
    70201 tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg
    70261 aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt
    70321 acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa
    70381 attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta
    70441 ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt
    70501 tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg
    70561 tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt
    70621 tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt
    70681 gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac
    70741 attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt
    70801 aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat tggttacaat
    70861 gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc
    70921 aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat
    70981 atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg
    71041 tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt
    71101 aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa
    71161 attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc
    71221 gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat
    71281 tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa
    71341 tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat
    71401 cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac
    71461 ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag
    71521 tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat
    71581 tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag
    71641 caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta
    71701 gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt
    71761 tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt
    71821 ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc
    71881 aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat
    71941 actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt
    72001 taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat
    72061 cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt
    72121 aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac
    72181 gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc
    72241 gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa
    72301 tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa
    72361 caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac
    72421 atgaccgacg ataaaaatgg ctatcccatt tgttttagcg gttttgagta gttctgcagt
    72481 ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt
    72541 ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc
    72601 gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc
    72661 gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat
    72721 ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc
    72781 accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc
    72841 taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt
    72901 tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc
    72961 ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac
    73021 atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc
    73081 ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg
    73141 gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac
    73201 ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca
    73261 aagttggtga attggaacat gatatggcct tcatttgcct cattattgta ataatcactg
    73321 tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg
    73381 ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg
    73441 gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt
    73501 tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct
    73561 tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct
    73621 ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac
    73681 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
    73741 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
    73801 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
    73861 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
    73921 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
    73981 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
    74041 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
    74101 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
    74161 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
    74221 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
    74281 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
    74341 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
    74401 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
    74461 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
    74521 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
    74581 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
    74641 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
    74701 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
    74761 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
    74821 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
    74881 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
    74941 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
    75001 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
    75061 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
    75121 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
    75181 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
    75241 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
    75301 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
    75361 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
    75421 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
    75481 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
    75541 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
    75601 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
    75661 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
    75721 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
    75781 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
    75841 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
    75901 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
    75961 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
    76021 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
    76081 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
    76141 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
    76201 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
    76261 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
    76321 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
    76381 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
    76441 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
    76501 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
    76561 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
    76621 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
    76681 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
    76741 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
    76801 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
    76861 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
    76921 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
    76981 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
    77041 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
    77101 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
    77161 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
    77221 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
    77281 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
    77341 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
    77401 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
    77461 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
    77521 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
    77581 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
    77641 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
    77701 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
    77761 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
    77821 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
    77881 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
    77941 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
    78001 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
    78061 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
    78121 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
    78181 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
    78241 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
    78301 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
    78361 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
    78421 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
    78481 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
    78541 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
    78601 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
    78661 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
    78721 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
    78781 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
    78841 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
    78901 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
    78961 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
    79021 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
    79081 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
    79141 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
    79201 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
    79261 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
    79321 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
    79381 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
    79441 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
    79501 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
    79561 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
    79621 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
    79681 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
    79741 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
    79801 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
    79861 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
    79921 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
    79981 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
    80041 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
    80101 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
    80161 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
    80221 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
    80281 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
    80341 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
    80401 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
    80461 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
    80521 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
    80581 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
    80641 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
    80701 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
    80761 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
    80821 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
    80881 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
    80941 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
    81001 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
    81061 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
    81121 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
    81181 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
    81241 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
    81301 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
    81361 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
    81421 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
    81481 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
    81541 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
    81601 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
    81661 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
    81721 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
    81781 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
    81841 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
    81901 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
    81961 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
    82021 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
    82081 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
    82141 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
    82201 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
    82261 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
    82321 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
    82381 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
    82441 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
    82501 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
    82561 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
    82621 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
    82681 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
    82741 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
    82801 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
    82861 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
    82921 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
    82981 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
    83041 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
    83101 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
    83161 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
    83221 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
    83281 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
    83341 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
    83401 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
    83461 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
    83521 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
    83581 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
    83641 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    83701 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    83761 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    83821 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    83881 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    83941 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    84001 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    84061 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    84121 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    84181 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    84241 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    84301 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    84361 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    84421 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    84481 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    84541 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    84601 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    84661 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    84721 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    84781 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    84841 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    84901 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    84961 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    85021 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    85081 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    85141 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    85201 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    85261 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    85321 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    85381 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    85441 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    85501 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    85561 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    85621 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    85681 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    85741 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    85801 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    85861 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    85921 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    85981 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    86041 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    86101 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    86161 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    86221 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    86281 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    86341 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    86401 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    86461 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    86521 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    86581 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    86641 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    86701 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    86761 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    86821 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    86881 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    86941 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    87001 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    87061 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    87121 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    87181 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    87241 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    87301 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    87361 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    87421 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    87481 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    87541 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    87601 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    87661 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    87721 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    87781 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    87841 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    87901 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    87961 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    88021 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    88081 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    88141 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    88201 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    88261 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    88321 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    88381 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    88441 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    88501 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    88561 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    88621 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    88681 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    88741 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    88801 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    88861 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    88921 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    88981 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    89041 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    89101 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    89161 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    89221 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    89281 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    89341 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    89401 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    89461 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    89521 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    89581 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    89641 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    89701 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    89761 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    89821 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    89881 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    89941 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    90001 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    90061 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    90121 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    90181 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    90241 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    90301 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    90361 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    90421 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    90481 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    90541 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    90601 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    90661 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    90721 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    90781 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    90841 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    90901 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    90961 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    91021 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    91081 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    91141 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    91201 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    91261 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    91321 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    91381 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    91441 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    91501 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    91561 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    91621 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    91681 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    91741 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    91801 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    91861 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    91921 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    91981 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    92041 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    92101 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    92161 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    92221 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    92281 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    92341 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    92401 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    92461 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    92521 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    92581 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    92641 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    92701 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    92761 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    92821 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    92881 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    92941 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    93001 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    93061 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    93121 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    93181 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    93241 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    93301 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    93361 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    93421 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    93481 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    93541 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    93601 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    93661 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    93721 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    93781 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    93841 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    93901 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    93961 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    94021 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    94081 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    94141 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    94201 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    94261 ataaa
//
LOCUS       DACDSR010000003        76945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDSR010000003 DACDSR010000000
VERSION     DACDSR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.003"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IWK15_000215"
     CDS             complement(12..1535)
                     /locus_tag="IWK15_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9545848.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IWK15_000216"
     CDS             1787..2968
                     /locus_tag="IWK15_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9545849.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWK15_000217"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWK15_000217"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9545850.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IWK15_000218"
     CDS             complement(5053..7764)
                     /locus_tag="IWK15_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9545851.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IWK15_000219"
     CDS             complement(7774..8475)
                     /locus_tag="IWK15_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545852.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IWK15_000220"
     CDS             8661..9260
                     /locus_tag="IWK15_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9545853.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IWK15_000221"
     CDS             9341..10354
                     /locus_tag="IWK15_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9545854.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IWK15_000222"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IWK15_000222"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9545855.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IWK15_000223"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IWK15_000223"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9545856.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IWK15_000224"
     CDS             11802..13175
                     /locus_tag="IWK15_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9545857.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="IWK15_000225"
     CDS             13532..15676
                     /locus_tag="IWK15_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9545858.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="IWK15_000226"
     CDS             complement(15728..16768)
                     /locus_tag="IWK15_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9545859.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="IWK15_000227"
     CDS             complement(17045..18847)
                     /locus_tag="IWK15_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9545860.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19604)
                     /locus_tag="IWK15_000228"
     CDS             complement(18861..19604)
                     /locus_tag="IWK15_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545861.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFMGASGSG
                     KSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESLTAQ
                     ENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARALITQ
                     PTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFIKDG
                     GIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19613..19975)
                     /locus_tag="IWK15_000229"
     CDS             complement(19613..19975)
                     /locus_tag="IWK15_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9545862.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20151..21023)
                     /locus_tag="IWK15_000230"
     CDS             complement(20151..21023)
                     /locus_tag="IWK15_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9545863.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21049..21561)
                     /locus_tag="IWK15_000231"
     CDS             complement(21049..21561)
                     /locus_tag="IWK15_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9545864.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21699..22529)
                     /locus_tag="IWK15_000232"
     CDS             complement(21699..22529)
                     /locus_tag="IWK15_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9545865.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22545..23153)
                     /locus_tag="IWK15_000233"
     CDS             complement(22545..23153)
                     /locus_tag="IWK15_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9545866.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23146..23463)
                     /locus_tag="IWK15_000234"
     CDS             complement(23146..23463)
                     /locus_tag="IWK15_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9545867.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23619..24347)
                     /locus_tag="IWK15_000235"
     CDS             complement(23619..24347)
                     /locus_tag="IWK15_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9545868.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24506..25387
                     /locus_tag="IWK15_000236"
     CDS             24506..25387
                     /locus_tag="IWK15_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9545869.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25624..26667)
                     /locus_tag="IWK15_000237"
     CDS             complement(25624..26667)
                     /locus_tag="IWK15_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9545870.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26670..27338)
                     /locus_tag="IWK15_000238"
     CDS             complement(26670..27338)
                     /locus_tag="IWK15_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9545871.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27480..29816
                     /locus_tag="IWK15_000239"
     CDS             27480..29816
                     /locus_tag="IWK15_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9545872.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29931..30242)
                     /locus_tag="IWK15_000240"
     CDS             complement(29931..30242)
                     /locus_tag="IWK15_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545873.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30423..31898
                     /locus_tag="IWK15_000241"
     CDS             30423..31898
                     /locus_tag="IWK15_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9545874.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31945..32529
                     /locus_tag="IWK15_000242"
     CDS             31945..32529
                     /locus_tag="IWK15_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9545875.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32531..32950
                     /locus_tag="IWK15_000243"
     CDS             32531..32950
                     /locus_tag="IWK15_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9545876.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33231..33515)
                     /locus_tag="IWK15_000244"
     CDS             complement(33231..33515)
                     /locus_tag="IWK15_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9545877.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33867..34577)
                     /locus_tag="IWK15_000245"
     CDS             complement(33867..34577)
                     /locus_tag="IWK15_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9545878.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34574..36046)
                     /locus_tag="IWK15_000246"
     CDS             complement(34574..36046)
                     /locus_tag="IWK15_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9545879.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36184..36657
                     /locus_tag="IWK15_000247"
     CDS             36184..36657
                     /locus_tag="IWK15_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9545880.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36810..37361)
                     /locus_tag="IWK15_000248"
     CDS             complement(36810..37361)
                     /locus_tag="IWK15_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9545881.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37466..37996
                     /locus_tag="IWK15_000249"
     CDS             37466..37996
                     /locus_tag="IWK15_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9545882.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38479..38922)
                     /locus_tag="IWK15_000250"
     CDS             complement(38479..38922)
                     /locus_tag="IWK15_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9545883.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38938..40098)
                     /locus_tag="IWK15_000251"
     CDS             complement(38938..40098)
                     /locus_tag="IWK15_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9545884.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40605..42305
                     /locus_tag="IWK15_000252"
     CDS             40605..42305
                     /locus_tag="IWK15_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545885.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42407..43240)
                     /locus_tag="IWK15_000253"
     CDS             complement(42407..43240)
                     /locus_tag="IWK15_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9545886.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43544..45709)
                     /locus_tag="IWK15_000254"
     CDS             complement(43544..45709)
                     /locus_tag="IWK15_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9545887.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45969..48263
                     /locus_tag="IWK15_000255"
     CDS             45969..48263
                     /locus_tag="IWK15_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9545888.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48256..48921
                     /gene="pgmB"
                     /locus_tag="IWK15_000256"
     CDS             48256..48921
                     /gene="pgmB"
                     /locus_tag="IWK15_000256"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9545889.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48914..49921
                     /locus_tag="IWK15_000257"
     CDS             48914..49921
                     /locus_tag="IWK15_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9545890.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49943..50962
                     /locus_tag="IWK15_000258"
     CDS             49943..50962
                     /locus_tag="IWK15_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9545891.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51243..52610)
                     /gene="nhaC"
                     /locus_tag="IWK15_000259"
     CDS             complement(51243..52610)
                     /gene="nhaC"
                     /locus_tag="IWK15_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9545892.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52720..54141)
                     /locus_tag="IWK15_000260"
     CDS             complement(52720..54141)
                     /locus_tag="IWK15_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9545893.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54384..56261)
                     /gene="tdc"
                     /locus_tag="IWK15_000261"
     CDS             complement(54384..56261)
                     /gene="tdc"
                     /locus_tag="IWK15_000261"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9545894.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56552..57808)
                     /locus_tag="IWK15_000262"
     CDS             complement(56552..57808)
                     /locus_tag="IWK15_000262"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9545895.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58388..59689
                     /locus_tag="IWK15_000263"
     CDS             58388..59689
                     /locus_tag="IWK15_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9545896.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59758..60357)
                     /locus_tag="IWK15_000264"
     CDS             complement(59758..60357)
                     /locus_tag="IWK15_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9545897.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60611..61492
                     /locus_tag="IWK15_000265"
     CDS             60611..61492
                     /locus_tag="IWK15_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545898.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61505..63115
                     /locus_tag="IWK15_000266"
     CDS             61505..63115
                     /locus_tag="IWK15_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9545899.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63192..63851)
                     /locus_tag="IWK15_000267"
     CDS             complement(63192..63851)
                     /locus_tag="IWK15_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9545900.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64134..64799)
                     /locus_tag="IWK15_000268"
     CDS             complement(64134..64799)
                     /locus_tag="IWK15_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545901.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64946..65551)
                     /gene="dhaL"
                     /locus_tag="IWK15_000269"
     CDS             complement(64946..65551)
                     /gene="dhaL"
                     /locus_tag="IWK15_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9545902.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65579..66562)
                     /gene="dhaK"
                     /locus_tag="IWK15_000270"
     CDS             complement(65579..66562)
                     /gene="dhaK"
                     /locus_tag="IWK15_000270"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9545903.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(66564..66959)
                     /gene="dhaM"
                     /locus_tag="IWK15_000271"
     CDS             complement(66564..66959)
                     /gene="dhaM"
                     /locus_tag="IWK15_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9545904.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67113..67433
                     /locus_tag="IWK15_000272"
     CDS             67113..67433
                     /locus_tag="IWK15_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9545905.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67545..68414)
                     /locus_tag="IWK15_000273"
     CDS             complement(67545..68414)
                     /locus_tag="IWK15_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9545906.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68510..69784)
                     /locus_tag="IWK15_000274"
     CDS             complement(68510..69784)
                     /locus_tag="IWK15_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9545907.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(69810..70319)
                     /locus_tag="IWK15_000275"
     CDS             complement(69810..70319)
                     /locus_tag="IWK15_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545908.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70494..72629)
                     /locus_tag="IWK15_000276"
     CDS             complement(70494..72629)
                     /locus_tag="IWK15_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9545909.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72712..74367)
                     /locus_tag="IWK15_000277"
     CDS             complement(72712..74367)
                     /locus_tag="IWK15_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9545910.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74369..74683)
                     /locus_tag="IWK15_000278"
     CDS             complement(74369..74683)
                     /locus_tag="IWK15_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9545911.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74853..75113
                     /locus_tag="IWK15_000279"
     CDS             74853..75113
                     /locus_tag="IWK15_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9545912.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75127..75441
                     /locus_tag="IWK15_000280"
     CDS             75127..75441
                     /locus_tag="IWK15_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9545913.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76171..76725)
                     /locus_tag="IWK15_000281"
     CDS             complement(76171..76725)
                     /locus_tag="IWK15_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9545914.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT        22821 a        15762 c        13830 g        24532 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgaattcg
    19501 cctttttcta catcgaagct gatgccgctt aatgcttgcg tctttttctc attgtttttt
    19561 ccgtatactt ttgttacatt ctgtacttcg actattttgt tcatttcttt ccctaccctt
    19621 taattagttt ttccagtgct gttttcggca cgtctttttt ttcgacttct tcatacgaag
    19681 tgacaccttt atttttgttc caggtgattt ttaaatatgc tcctttacga agcggtcgag
    19741 tcaattgtcc cttgaagtcc agttcttttt ccttaccctc tttcgtatag ccaggcagga
    19801 tatatctata gttgactgtt tcttgtccag agtctgattt tgaaactgtt tttgtaccat
    19861 ctgtcgtaat ttgtacatag tattcttctc cacccataac tacgtaatct gcgattttca
    19921 cccctgcaag taccacaacg atgaaaacaa gtagaccgat cagaagtttt ttcacttttt
    19981 ccccttctct cttactttat atactgatta tagaaaaaag cgaacaattc tgccttcgaa
    20041 tcaactatca ttcttctttc aattttgtca ttttctttaa aacaactaaa aaaagagttc
    20101 aagacaataa tccgtcctaa actccttctt cctatcttac aaaaagtatg tcacttacgg
    20161 tctttcatgt cgcctgtatt gataacaggt tgcgttcctt tgtctgtaat gattgagaca
    20221 agtaaattat tgatcaattg gactttccga tcatcggtga aattgatttc ttgtccatct
    20281 tcgatttgct ctaaggccat ttgagtgatt gtgacagctc cttcaacaat ggtttgtcga
    20341 gcagacaaaa tggcttttgc ttgttgacgt tggagcatgg cactagcgat ttctgttgca
    20401 taggctaaat ggttcaagcg tgtttcgatt acttcgacac ctgctactgc taaacgctct
    20461 tgcagctctt tttgcaactc gtcagacact gccgtcgtgt ttcctcgaag cgtcaaatca
    20521 ttctcattga atgtatcata cgggtattgg ctagcgatat gccgaatggc tgtctcactt
    20581 tggatttcta caaaatcttg ataatacgct acatcgaata atgcctttgc tgtatcaacg
    20641 actttaaaga ctactactgc tgagatttcg atggggtttc catccaaatc attgactttt
    20701 aaaacagaac tattgaaatt ccgaactttc aacgaaacag tcattttttg tgccaaaggt
    20761 atcgtaagga aaaagccatt ttctcgaatc gtacctaaat attgtccaaa aaacaaaatt
    20821 acttttgctt gattaggact gactacagtt gccgaactta acaacaataa cgtgatcaac
    20881 caaagaaaaa tacttaaaaa aagtgcccaa actttcgctt gccacatacc taaatagaag
    20941 aaaaacaatc caactaaagc taatacgacc aatccgacaa tccctgcata accattgata
    21001 taaaatgctc ttttttctct catgctactt cctgcgctcc tttatccttc aataaatatc
    21061 taatgttact tttttaattt tttttcgaat acgcgccatt acaccttcta gttctaactc
    21121 tttgtcttca gtgatgtacc aaccccttgc ctgtgtccct ccggctgcct gcggtaaata
    21181 atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta ccaagacctc
    21241 tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga taaatcgttc
    21301 cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat aataatccag
    21361 ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct ctaattggcg
    21421 accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat catagaaggt
    21481 ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta acttttctct
    21541 ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga tattttcttt
    21601 cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt ggaaggatct
    21661 ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc attgtcctct
    21721 tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt tttctgtcct
    21781 tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga ttcaccagac
    21841 gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc accgtttagg
    21901 atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct gttttcacct
    21961 gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa ttcatctttc
    22021 gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg tgtcttagta
    22081 ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc ttcgattgtc
    22141 aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact tgtgtcttct
    22201 aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac gccatttttc
    22261 tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa atccaatccg
    22321 cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc atccttgatt
    22381 ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt cagactcttt
    22441 tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat cagaccaata
    22501 ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac gcccgctgaa
    22561 tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt catcatgaca
    22621 taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc gcttcctgtg
    22681 gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc aaacacactg
    22741 attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa aacatagata
    22801 ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat aaaccccagt
    22861 gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca aatcaggcga
    22921 atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc tgaaaagtaa
    22981 ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat gatctgatct
    23041 aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc aaaacgatcg
    23101 tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt catttgtgct
    23161 ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa taatttcttc
    23221 taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc cttcaaaagg
    23281 ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg gatatagtgt
    23341 agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc catacatatc
    23401 ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa tctggatgtg
    23461 cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat tatacaacat
    23521 ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa gatttgatcc
    23581 tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg cataatccaa
    23641 tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat gtcagctact
    23701 gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa tccgccgcaa
    23761 gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt tgccaataag
    23821 aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac ataccctaaa
    23881 ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat caaaatcgta
    23941 ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact tgcacaaaat
    24001 agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc aagcttttca
    24061 ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt tatcccatta
    24121 cctaaatgaa caatcagacc aatcgctgtt cctatcacta aagcaaaaat aacagccggc
    24181 atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac accaaaaacc
    24241 atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc gccaattagt
    24301 ccgccaagca aaacggaacc tacattgatc aaaactccag ttggcatcaa aaaactcctc
    24361 tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct ttcgctgcaa
    24421 gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta aagtattctt
    24481 tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt ttcttgattt
    24541 agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc aaggaaatat
    24601 ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg aacgcgccca
    24661 tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa aaaatgtcgt
    24721 agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca acaatctttc
    24781 attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca aaatcaccga
    24841 gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa gtgtcgaatt
    24901 aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa atttccaggc
    24961 cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag cagttctacc
    25021 taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga aaaaggagaa
    25081 ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc tgtgtcgcga
    25141 agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga tcatcaatat
    25201 gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga atctattaaa
    25261 agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg catttactcg
    25321 taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa atcaagaaga
    25381 aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc gacgcatgac
    25441 tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca tttctcgtag
    25501 ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt ccctaatcac
    25561 aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt tttattcttt
    25621 ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga tactgctata
    25681 atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc ctagcccatt
    25741 cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc gatctctggt
    25801 ctcttcattc attcctacac ctgaatcggt cacttccagt ccaatctctg tggctgtctc
    25861 aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt tatccatgat
    25921 attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg cagcaagttc
    25981 gatatccaat tctagctgtt ctttctccca tttaggctgc aaaaaaacga ttacttctct
    26041 gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt tttccagttt
    26101 gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt cgataatccg
    26161 atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca attgaacata
    26221 tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca caaagtcatt
    26281 cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt tatacagttg
    26341 ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc ccgttgctac
    26401 cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc caacaaaaat
    26461 cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg ggtataacgt
    26521 tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga aaataaaacc
    26581 gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa aatagatcca
    26641 taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc tagtccccgt
    26701 actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg tttgatatgt
    26761 acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc catcaactgt
    26821 tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag aaattctttt
    26881 tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag ctctaatttt
    26941 gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag caatgcttcc
    27001 acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc tgctcctaat
    27061 ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag aacaggtgtg
    27121 tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt cggcatcatg
    27181 atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc atcctctcca
    27241 ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta aagcaactgt
    27301 aattgcggat catcttctac tactaaaata cgattcattg atctccctct gttttttgaa
    27361 aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg tatactacga
    27421 tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga agggtgatta
    27481 tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc accaaagcat
    27541 tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta ggtcctagtg
    27601 gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat gattccggtg
    27661 atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg gatgcctacc
    27721 gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat ctaggcatta
    27781 ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa aaacgaaaac
    27841 gggcagaaga tgcattgcac cgagtcggct tgacagatca tatgcataaa aagccaaatc
    27901 agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc aatgacccag
    27961 atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt atccagatca
    28021 tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca cataatcctg
    28081 aactggccca tcaatacgca gatcggatca ttgaattttc tgatggtaag atcttaacag
    28141 attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc agaacaaaga
    28201 tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa aaaggacgta
    28261 ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc gttctgtccc
    28321 tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg gcgaaatttc
    28381 cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga ttgggtgcaa
    28441 gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa gaagataaag
    28501 cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac atcgatccta
    28561 acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta cgtgatgtaa
    28621 atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa tctctttctt
    28681 tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct acacagcttg
    28741 acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga agttatccag
    28801 cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat atcaacgcat
    28861 tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat gatatcgttg
    28921 gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca actgggaatt
    28981 ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa ttgaagatct
    29041 cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca ggaatcgctt
    29101 atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa atcgtcaaag
    29161 ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa aatgccaaac
    29221 aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg atttatccga
    29281 acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat aaaggcaaat
    29341 cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa ctgactggcg
    29401 gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct ttagtaacaa
    29461 gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca aaagaaatcg
    29521 gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt gacgcagaaa
    29581 cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg ctagctacgt
    29641 tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct caactaaatc
    29701 ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt ggcggtcatc
    29761 ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca gaataaggtt
    29821 gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa acagcaagaa
    29881 acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt ttataattct
    29941 ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc ttttccattt
    30001 atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat attacgagaa
    30061 agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa ggcatcggtt
    30121 atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa tgtaactttc
    30181 aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctt catttttttc
    30241 actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat atcatagttt
    30301 attctattat caaaaaaacg catccattcc attgtagaat actttaccga acaagttgaa
    30361 ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag gaggaatctc
    30421 tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa acacttccag
    30481 gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct ggtgtttacc
    30541 gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca gctatgactt
    30601 tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg gctagtcgta
    30661 aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc ttgaaagacg
    30721 ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca gcacaagaag
    30781 tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct gcacctacaa
    30841 atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga tcagatccag
    30901 aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa gatacgatcg
    30961 gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa ttcgaagcct
    31021 tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt gccattggca
    31081 cgactttcga accacctacc actccacttg gctacaatga actatggtgt gcaggagctt
    31141 tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg ggtattgctc
    31201 gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa gaagatatca
    31261 acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat gctaatccag
    31321 ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca atcatgcaaa
    31381 caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca gaagcaagta
    31441 ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac aatcctcctg
    31501 caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa gcggatgaag
    31561 gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg aagacagtca
    31621 ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg actttcaaca
    31681 ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac catccattgg
    31741 acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg atgagtgcaa
    31801 aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta cgtgcaaaat
    31861 tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc aactgttttg
    31921 acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa aaaaatcgtg
    31981 attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa gaaactaaaa
    32041 gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca gaacttaaag
    32101 ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca ttattcaaac
    32161 aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat gatcatcgta
    32221 ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa cctgattgca
    32281 cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc tcgtgaaacg
    32341 ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg tgtgcaaatc
    32401 ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct tgttaatcac
    32461 catacaatga aacttttgga tcaatttgga attgtaaatg agcattcaaa acgttggcag
    32521 ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt tcacgatcca
    32581 agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg gagatacacc
    32641 acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg tacgtttccc
    32701 tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag ctgtgatcat
    32761 caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg accatatctc
    32821 caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca agattttaga
    32881 ttggttggaa aaaatagatt tccatatcaa agaaccagtt tattataaag atggtgaaaa
    32941 aacattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga gagaaaaaaa
    33001 tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga cagctgattg
    33061 aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta agaaaaattt
    33121 tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga aactggcttt
    33181 tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt ttatttttgc
    33241 ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg aatatctttt
    33301 cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat attcacgata
    33361 ctgccttcat acggcatatt gtcaaaagtt gcttggactt taactcttcc agcaccgaat
    33421 tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgtcgac ttcggaagca
    33481 tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt ttttacatct
    33541 agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca gttccttttt
    33601 tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcaacaaaca
    33661 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca ggctcacaac
    33721 ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa tcagatgaaa
    33781 aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc agctttttca
    33841 caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac taattttgcg
    33901 aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg ctctgctttg
    33961 ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata agcttttgct
    34021 tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc tgcttggtcc
    34081 gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct gtcatattcg
    34141 tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta ttcccaataa
    34201 gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt tgtttcgtta
    34261 tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag atacactttt
    34321 tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga atcagcactg
    34381 gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg attggacaaa
    34441 ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa agataaaaag
    34501 gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa gctgacaatc
    34561 aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct tttttcttca
    34621 ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata aacgcggcat
    34681 ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc gtattatcca
    34741 atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg atcaaaatag
    34801 ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc ccatgactga
    34861 tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg accatggaga
    34921 tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc gtttttgtca
    34981 agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg gctaaacgtt
    35041 tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc atgatcaatg
    35101 cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata ttttgaatat
    35161 acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca gctaaaacag
    35221 ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga tgttccaaat
    35281 gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag aaaccaatta
    35341 ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt aaactaccga
    35401 atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac aatggaataa
    35461 cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc agtaccatac
    35521 caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta gggcctaaag
    35581 caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt ttttctggcg
    35641 ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccacg ccgcaagctt
    35701 ggatcaagcg accgattagt aatgtactaa aattaggtgc tgtaaagcaa gtaatcgttc
    35761 cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat ttattgatca
    35821 gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc gttaaccatt
    35881 gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct gtcgtcaata
    35941 aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact aaaagtgtcc
    36001 gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt ctccttcttt
    36061 ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt tgtcaaccta
    36121 cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa aggagtgaca
    36181 aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt tgttggcatc
    36241 agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg ggaacaaaag
    36301 ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg tttacctaca
    36361 gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt gtcaaaagct
    36421 gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt tttttcaaaa
    36481 gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat cggtaacttc
    36541 aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga attaacttac
    36601 aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga actctaaacg
    36661 ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa tacttgaaaa
    36721 aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc atatagaaag
    36781 gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc ttctaataca
    36841 tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa aatcaggtag
    36901 tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt tcgctctaat
    36961 agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc tactaatgcc
    37021 ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg attaaccggt
    37081 agttcattgg ttggtgtatg aatccactct gccaattgtt ccttcccttc ttctgttaat
    37141 tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg aatgattcct
    37201 tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg ccaaaattct
    37261 cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt cttacttaat
    37321 aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc ctccttgaca
    37381 aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt attacatgtt
    37441 ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag attttattgg
    37501 aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg ccaaaaatga
    37561 ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg taaaagacca
    37621 agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga cagagccaac
    37681 agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg gaatgatctt
    37741 tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa acgaacatat
    37801 cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac ttgttcctga
    37861 atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg ttattagcga
    37921 agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg atgaaaatta
    37981 taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct gacctgagta
    38041 ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag ctaataccga
    38101 gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta tgctttgagt
    38161 aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg gcttattacc
    38221 gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt tttgacctga
    38281 gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt gagctaatac
    38341 cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat attcacacag
    38401 ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc tgggagcatt
    38461 atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca caagttcctc
    38521 ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg aaagatagaa
    38581 atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat tggtttcttt
    38641 tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt tcacaaagat
    38701 tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat ctatttctgc
    38761 taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat tctcccaata
    38821 agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg atagaacaga
    38881 ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc tccttactta
    38941 ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt ttcgcccctc
    39001 aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag caactttagg
    39061 atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct tgactttccc
    39121 atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt ttttcttatg
    39181 tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa taataaatga
    39241 aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga taaacactaa
    39301 cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca tggttctttc
    39361 ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc gatgtcctgt
    39421 cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg taactaagtc
    39481 ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt gtatattttt
    39541 aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc cgttttctaa
    39601 acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag agatcactga
    39661 atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg ctccattttc
    39721 aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct taggtatatt
    39781 gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat ttctgttctt
    39841 ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt gttttttctc
    39901 ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta cttgttcgat
    39961 acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata aagcgatcag
    40021 taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac gagactgttt
    40081 ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt tcttttatta
    40141 tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa agctgtcctg
    40201 catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt gggttgggct
    40261 tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aaactgcgtt
    40321 atgctttgag taataagagc gctagtcaga aaacagcttc tcatattttc taggctagtg
    40381 agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat gaaaaagcta
    40441 ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa caaaacttat
    40501 gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt cataaatatt
    40561 ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg aatttatcta
    40621 aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca atttttttca
    40681 ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg ggtgctgatg
    40741 ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat ggggatttcc
    40801 attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat gccgtttacg
    40861 gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc tggtttcatt
    40921 atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg ctgattttac
    40981 tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta ttcatgagta
    41041 cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg acttgttttt
    41101 atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc cctgttatca
    41161 aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg atcatgttag
    41221 ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa aaaggaaaag
    41281 cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca aatatttggg
    41341 caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt aacagagaaa
    41401 tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct aaggcctttc
    41461 ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag aacaccttat
    41521 tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact agcttgattc
    41581 catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag tttccatttc
    41641 gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca ctgaaccagt
    41701 ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt ttttcagcac
    41761 agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca tttgtcagtc
    41821 gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc agcaaagata
    41881 tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca atctataagg
    41941 agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac gaaacaactt
    42001 ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact ggggacaaaa
    42061 ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat ggaaaaacat
    42121 tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagaaa aaaggaatca
    42181 acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt tctcttactt
    42241 tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag cttttacgtg
    42301 cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta taatcgtttg
    42361 gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat tcgttcacat
    42421 ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc ccatcaaaaa
    42481 tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct atacgcaatt
    42541 tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct tttccttttt
    42601 cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca gtataataac
    42661 cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg cgagtttcca
    42721 aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta tctgtccacc
    42781 aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt ttcccaatca
    42841 agatcttgcg tgtctgataa tcttctttct tttgagattg ggagacggta aggacctgac
    42901 tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca taaccgatgt
    42961 ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc tgaactaaac
    43021 ggtaggcaaa attatcttta cggataggta cttgattctt tacctgacag aacttcatta
    43081 gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt aaagcaatca
    43141 cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc tctggttctg
    43201 gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc cttgtcttca
    43261 tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca aatagtgttc
    43321 tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg tttggctgtt
    43381 tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt attcaaactg
    43441 ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt ttgtttggct
    43501 gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt ttaatggagc
    43561 cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct tctactttat
    43621 cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc acatcaagat
    43681 ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca gcaatctcaa
    43741 aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact tcgataccat
    43801 ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact tttccagcga
    43861 tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc atcatttttt
    43921 gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa gctgcggaaa
    43981 ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt cctgtagaaa
    44041 ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata tctgatttca
    44101 agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg atcaatccca
    44161 tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca tttacactta
    44221 cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct cccagcaaat
    44281 gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat acaacggttt
    44341 cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg aattttttga
    44401 tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac aacacgaagt
    44461 tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc agcaattcga
    44521 tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat gcagcccctt
    44581 ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg aactccaatg
    44641 gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt gatttatatc
    44701 ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc atcaagatac
    44761 ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta tgcaacacaa
    44821 actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg gcccaagcca
    44881 accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat aaaatctcat
    44941 acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc aatccaaatg
    45001 gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt gcgctttctt
    45061 gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt ggccacacca
    45121 cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga acaaagcgct
    45181 ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac ttattataag
    45241 ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc aaagctggtg
    45301 cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac agagtgtggg
    45361 taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc ccagtgatcc
    45421 gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt tcttttgccc
    45481 aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt gcccaaccaa
    45541 tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg atcaagggaa
    45601 ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc acaaccatta
    45661 aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt ccgtttagct
    45721 cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt ttgtaaatgc
    45781 tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt gtgttccctt
    45841 ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat attttctttt
    45901 gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag ctcattagga
    45961 ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa atcagcacga
    46021 cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt ggaaacggtt
    46081 atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat caaggtactt
    46141 atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa aatggttatc
    46201 cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat atttcgatca
    46261 acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg gaattagata
    46321 tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat aaagtacgca
    46381 tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc tatttaacta
    46441 tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat ggaaatgttc
    46501 aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt ggaactatag
    46561 aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt gaacaattta
    46621 ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct gactatcacg
    46681 aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt gagacatggt
    46741 cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg cagcaagcag
    46801 aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca aaagaagagc
    46861 aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa ggagacgaag
    46921 aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac tacggcgaag
    46981 atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc ggagccactt
    47041 actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa cctgaagtaa
    47101 ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac aacgctcagc
    47161 aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc gaatgccata
    47221 acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc tatgcaatct
    47281 acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga ctggaagttt
    47341 tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga gcaaataaat
    47401 acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat aacaattggt
    47461 atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat ttaacttatc
    47521 aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa gatatcgcag
    47581 agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat gacacattct
    47641 tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct ctgaatcagc
    47701 attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat gtactacagg
    47761 ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac tttgatttct
    47821 atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg attttagcag
    47881 cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct cgcttggatt
    47941 tggataatta caataacgac acagaagacg gcttgcacat cacttccatg actggtagct
    48001 ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag ttgagattcg
    48061 caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat cgcggacgtt
    48121 tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa ggttttcctc
    48181 tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa ataacgctaa
    48241 gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt cattaccgat
    48301 actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg cattacgatc
    48361 gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact ctctttgctt
    48421 ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga attagcgaaa
    48481 agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga tgtcttcccc
    48541 ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc tcttgcttct
    48601 gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta ctttgatgct
    48661 attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt cctccttgca
    48721 gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc gcaagccggt
    48781 atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc tgaagacctt
    48841 ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta cttgaaagaa
    48901 aagtgtcatg accatggata atccagagat ctccgtcgta ccagttccag agactgaaat
    48961 aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt acggtgcccg
    49021 cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt tattgtctta
    49081 cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg tcggacctgt
    49141 agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa actgcggatc
    49201 acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag tagaagtttt
    49261 caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt ctacctatcc
    49321 aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag agatgacaac
    49381 ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct acttcaacct
    49441 ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt tagatggtgt
    49501 gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag aaaaactgcc
    49561 aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc cagatggact
    49621 agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac aattattcga
    49681 gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg tattattttc
    49741 tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt caaactacgg
    49801 attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat ttggatcgat
    49861 caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct ctgcacaata
    49921 acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga aaaaagccgg
    49981 tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt ccgagacaac
    50041 aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca tgactgcccg
    50101 caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc ttgattcaaa
    50161 agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg aagaagcgcg
    50221 gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc tgttagagaa
    50281 cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt attcagatag
    50341 tcaagatcct gtcattgatt atttatacca aaaccacatc cctttcactt tgatcggaca
    50401 gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc ttctaggtaa
    50461 acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg tgacaaacac
    50521 gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa tgatgatggc
    50581 tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc tgtcctttga
    50641 gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata tcttcgccct
    50701 tcgcacgatg cagttggcgc aaatgtacgg ctatgtcatt ccagagacac tctccgtcat
    50761 tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa gtatcgatat
    50821 cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga tccatgaaga
    50881 attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag agacagtcgt
    50941 tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca tttatcctat
    51001 ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc tgtaaaaaag
    51061 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
    51121 aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa aagcaatttt
    51181 cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttccttc
    51241 cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt aaagacaggt
    51301 gcaagaatcg agaaaaagac gaatggcaag taatgcaacg cgctaacttt catcgttccc
    51361 ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc acctgaatcc
    51421 gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga acgcttgaat
    51481 gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt gattccgata
    51541 cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc ttttaattct
    51601 tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc caatgcaagt
    51661 aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag cagttcatct
    51721 agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc tgcttttccg
    51781 accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga tcccgctagt
    51841 aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac tagtggaatc
    51901 aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc attgatacta
    51961 gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa cgaaaggacg
    52021 aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt aactcctcca
    52081 atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc taaaaaggca
    52141 ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg accgatcccc
    52201 aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt cactccgaca
    52261 attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag tttaaatcca
    52321 taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa gacaccaatc
    52381 aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat cccgtcatgg
    52441 atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat gaaagctgca
    52501 aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag gagtgccaac
    52561 aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat cgtcgatctc
    52621 ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg acctcctact
    52681 aaatcagcga caaaacgtcg gctgaaagac ttattttttt tatttttctt ctaaactttt
    52741 ttctacctca atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa
    52801 catgaccact gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt
    52861 agcaaatacg acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc
    52921 gtttccgttt ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt
    52981 aagcaatgaa acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt
    53041 aagtgtcaag aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga
    53101 aataacgatt gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg
    53161 gaatccgaat tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg
    53221 tccagtaacc caagctgata attgtaaaag cacaccaata aataccatga agctgaagat
    53281 atttccgatc acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat
    53341 atttgctaat tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac
    53401 attcaataaa actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc
    53461 gtctttcaaa cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat
    53521 accagctaag tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga
    53581 aaagtcacct agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa
    53641 aacgaacatg atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac
    53701 ttttgccata tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca
    53761 acctaaaacg aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa
    53821 tggtcctaaa gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca
    53881 tgcaacgaca aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc
    53941 ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa
    54001 caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat
    54061 actccgaacc gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt
    54121 acttacttca gatttactca ccgttgacac tctccttgat tttttggata aaataacggt
    54181 gtctttgaag tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt
    54241 tgagtctatt gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc
    54301 ttaggtctcc ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct
    54361 gttttctaac atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag
    54421 attttttcca atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt
    54481 tcttctttgt tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca
    54541 cggttccatt cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca
    54601 tagtctggga tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct
    54661 ttcacgtatg aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca
    54721 tcgttgcctt tttctttgaa tacatagtct accatattga aatctggata agttaatgga
    54781 tgaacttcga tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa
    54841 cgatgtgcac cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt
    54901 aagacatggt gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag
    54961 atataagccc caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag
    55021 taagagataa cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga
    55081 acatagccca ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat
    55141 gcactgtgta cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa
    55201 tgaacatctt ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca
    55261 cggccgtatc cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct
    55321 aatacgcgac gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat
    55381 cctacaacac cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt
    55441 ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg
    55501 tctaaaccga taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga
    55561 accaaccatt ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt
    55621 tcgtcgattt tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc
    55681 attaattccc aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc
    55741 ggcaatgatt tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca
    55801 gcaacgatgt ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact
    55861 tcttcttcca tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat
    55921 agcatagcaa aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc
    55981 cagtaacgtc cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct
    56041 gatagaacat cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct
    56101 ggcgtaataa taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc
    56161 accaattcat tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat
    56221 agggcattta ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa
    56281 aatatttaaa ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt
    56341 ttggcctgat ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt
    56401 gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt
    56461 aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt
    56521 tccctttttc aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat
    56581 ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga
    56641 caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt
    56701 cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt
    56761 caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg
    56821 catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca
    56881 agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac
    56941 gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga
    57001 aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt
    57061 agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc
    57121 cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctactt
    57181 ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg
    57241 ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt
    57301 gcagaatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga
    57361 tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa
    57421 gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac
    57481 caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg
    57541 tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc
    57601 caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa
    57661 tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc
    57721 tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc
    57781 gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa
    57841 gcaggaacct gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa
    57901 aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc
    57961 tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga
    58021 ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg
    58081 aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt
    58141 ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct
    58201 cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa
    58261 agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc
    58321 ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac
    58381 gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt
    58441 tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat
    58501 tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt
    58561 tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga
    58621 aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga
    58681 gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag
    58741 cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta
    58801 tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt
    58861 tttagttaat ttaaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg
    58921 gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta
    58981 tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat
    59041 caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct
    59101 gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt
    59161 ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga
    59221 caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa
    59281 agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact
    59341 agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa
    59401 aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc
    59461 agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga
    59521 tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa
    59581 tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt
    59641 catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat
    59701 ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta
    59761 tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag
    59821 cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat
    59881 caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag
    59941 atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt
    60001 tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt
    60061 gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc
    60121 cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata
    60181 aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt
    60241 ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa
    60301 agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg
    60361 tagagcctcc cgtaatttta aacttttata atatatatag ttcaaattta ttctgaggtt
    60421 tattataact atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg
    60481 acagaaattt ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg
    60541 tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag
    60601 tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt
    60661 cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg
    60721 accaaatggt tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga
    60781 cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga
    60841 acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat
    60901 catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat
    60961 tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca
    61021 aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc
    61081 tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa
    61141 agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc
    61201 ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac tcgatgaatt
    61261 gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc
    61321 acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga
    61381 taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga
    61441 atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag
    61501 cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga
    61561 ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc
    61621 tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa
    61681 cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt
    61741 aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc
    61801 tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat
    61861 tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct
    61921 gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc aagcagattc
    61981 gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg
    62041 gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct
    62101 gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc
    62161 ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat ttacaactaa
    62221 caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt
    62281 tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc
    62341 gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat
    62401 gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg
    62461 gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg
    62521 cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc
    62581 tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa
    62641 tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc
    62701 tattctattt gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt
    62761 ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa
    62821 tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc
    62881 tcgaatgggt aaacttgctt atatctactt agcttactcc tttgcactca attactttgg
    62941 cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg
    63001 gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc
    63061 actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa
    63121 aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt
    63181 cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc
    63241 tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg
    63301 gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc
    63361 cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc
    63421 atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt
    63481 ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg
    63541 caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac
    63601 tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta
    63661 gcagataatt gatacacttt catactgccg gaggccacga tcactaattg aggttctcca
    63721 ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact
    63781 ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga
    63841 tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc
    63901 ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc
    63961 agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa
    64021 gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt
    64081 cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga
    64141 aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt
    64201 atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt
    64261 cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac
    64321 aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata
    64381 tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta
    64441 ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga
    64501 ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc
    64561 caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg
    64621 ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag
    64681 tgttctttat attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc
    64741 tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt
    64801 gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag
    64861 aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat
    64921 gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa
    64981 ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat
    65041 aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga
    65101 tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca
    65161 tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga
    65221 tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca
    65281 tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg
    65341 ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt
    65401 cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg
    65461 tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc
    65521 attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt
    65581 accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt
    65641 cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc
    65701 ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga
    65761 actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta
    65821 aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct
    65881 gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat
    65941 cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta
    66001 gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg
    66061 aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc
    66121 cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac
    66181 tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat
    66241 cccctgtata attcttaacg atcagcaaaa ctccttttcc actatccgct tccttaatag
    66301 ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta
    66361 acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa
    66421 ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat
    66481 gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg
    66541 gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct
    66601 gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct
    66661 tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct
    66721 tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct
    66781 gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca
    66841 tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt
    66901 tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca
    66961 agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa
    67021 ttcaagtata taataaattt ctaattttgg gaagatatgt gtagtgcttc ttgaagtttg
    67081 ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag
    67141 agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc
    67201 atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct
    67261 atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa
    67321 aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg
    67381 acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg
    67441 acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt
    67501 ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg
    67561 atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa
    67621 gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt
    67681 tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa
    67741 atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta
    67801 gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc
    67861 gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg
    67921 aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct
    67981 ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga
    68041 tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct
    68101 aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca
    68161 gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta
    68221 tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag
    68281 cttatagttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt
    68341 cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc
    68401 atcacttttc ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg
    68461 gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg
    68521 tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa
    68581 tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg
    68641 cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact
    68701 ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat
    68761 aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta
    68821 cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg
    68881 ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt
    68941 ctgttccacc ttttgcccaa cgttttcttt gtcgatatag catcttcaac gtcacaggta
    69001 cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat
    69061 cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc acatcgatca
    69121 aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag
    69181 ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg
    69241 caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg
    69301 caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt
    69361 ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt
    69421 gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca
    69481 gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat
    69541 agttgtgata attcaatttc gtcatcaaat actcgattgt atcttcgatc acaatttctt
    69601 cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc
    69661 attctttttg cttatgtttg tataaaaaca cataacacca gacaccaata aaccatacaa
    69721 atccgcctaa aatcggataa gaaacgagaa aaaatacata atagacaaac aagtatctaa
    69781 gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt ttctttatac
    69841 aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata aaaacggacc
    69901 gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag taattcttgt
    69961 ctttttttca atttttcatc ttcatattca aaagatttat ttactttttt gtatagattt
    70021 cgattgttcc acctagtcaa tacaaatgag gtaatcagga aactgaaaaa aatcaataca
    70081 aaaaagccga ttaagaaata cagcattttc ttttctgata aaaaagcgta caaggaaaaa
    70141 tccttggctc tttctggtct cgttaatggg aaaaagaccc atataaaagg aagaacaacg
    70201 atcatccatc ctaaaagagc cactaacgtt tgccaaatct ttaaccagta attacctttt
    70261 gaaaaatact tatctttcac caaaattgat gcaacttctt ctcttgttac ttttttcata
    70321 tcctcacctc acgttttttt gttacgacgt aggagtttac catacatttc atttcttttc
    70381 acataaagtg ttcctaaatt tttcctaaga aaaagaacaa aacatttctc atcagcaaat
    70441 gaaaaatatt ttgttctttt tatcagttgt tctttttatt ttttatctct taattatgtt
    70501 gctctttcca atacgacaat gcttttcatt cctctagata tcgccgtgta catttgtctg
    70561 cgtcttcttt gctcatctcc atcgttttca aatggatata agattacatg atcaaattcc
    70621 agtccttttg ctagactcaa aggaatgatt tggcagtttt ctaatccata tgaatttttt
    70681 ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt gggtgataac tgccgctttt
    70741 tctcctttta gagaaaccaa aatctcagct agtattttca acaattcttc agaggatcga
    70801 aaagagcggt attcgggttc ttttccttgc ggtcggatcg atacgatgtc gatcttctct
    70861 tgatccacag cataggtttt gaaaagctct gtgattgctc cgctagaacg gtaactattt
    70921 ctaagatcat accgatggat cggcagatgg tagtcgtcga aacaacgcat aatatcagca
    70981 aaagtagtgg aagaattaaa gattgcctga ttctcgtcac ctactaaagt aaagtgagtt
    71041 tttggaaata attcgatcaa tagacctaat tgtgccaggg tataatcttg tacttcatca
    71101 atcaatagat agcgaaatgc tggtacattt accttttcaa caagtaaatg acgaatagct
    71161 aatataatga ctgcttcatc aacagtacgt gtgggttgcc aagcataagc tgagccatat
    71221 cgtttttcat aaattctttc aaacaactga tgctcatcca cccatgccat ttcttcgatt
    71281 tgtctggtta tttttcgata ttttttttgt aaaagtttcc ttgcataagc tacaatactt
    71341 tgttccgaat catcagaaat caatttaccg aataattcca actgcctatc ttctgtcaac
    71401 gagagtacct gattctgcac agcaggactt tttgcttgtt tcaaaagatg attcttccat
    71461 tcgctcatca gtgccttttt cgttgcttga agcctatcga tcaatttagg atgagcagga
    71521 actttctcat atattttttc gatcgttttt ttagaaagga ctgtttttcc atttttagta
    71581 agtccacgga taaaattcgg atgatctaaa aacaaaggat ctgattgttt tagattatcg
    71641 ataaactttt tgctacgcaa acgttctgtc tgctccgata cattttctcc cgtgatacgt
    71701 ttgaaataag cctcttcacc ttctatttcc tctgctgaaa gctgtgaaac cagctgtatg
    71761 atggttagat tcagtggatt tctttcacct aatgatggta ggacattcga aatatagtcg
    71821 ataaaccgat gattaggtga tagaatcagc aagtcatcgg atgtcatcgt atctcgatat
    71881 aaatagagca gatacgcaat acgctgcatg atagcagagg ttttaccact tcctgctacg
    71941 ccatttacca agatattttg acttttcaca tcccgaatga ttgtgttttg ttccgattgg
    72001 atcgtagcag tgatagcctt catttcattg gtctcatctt ggcctaagac ttctaataat
    72061 acgtcatctt gaatagctac agacgtatca aaaaacttga ccaaacgatc tctttccaaa
    72121 agaaattgtc gtctttcttt aataaccgta tcgattttat tttgacgaac cgtataagaa
    72181 gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg gtgaacgcca atcataaaca
    72241 agattttcat ctgcatcatc tgtgaaattt gctgtaccga tataaaagga ttcttttggc
    72301 tcattatcca gaaagtctac aacgactttt ccaaagtagg gacttttcaa taaacgttcg
    72361 atctttgcta gctgattttc tgcgtttttc tgtttgatat tcatctgatc gatttctcta
    72421 tttttcattt caatcatcga aaaagtatcc aggttatcta agtaactgtc aaaattcaat
    72481 cgaagctcgc cagccatttc tttcaacgac tggtttcctt ctatttttgc cgtatccatc
    72541 gattcccgca gttcgttttt tttatcaatt agcttttgat acacgttctt cagatatgct
    72601 tcttctaact gtttttgtgt gttgaccata cacattctcc tcttttttta aagtggtgga
    72661 accgattagt ttacctcttt taagagtcga atgcaagctt tttaacgagt atcatttgtt
    72721 aggatatatc agttcttcag gtcggacagt ttgatataaa gaaaatgcac catcaaggtt
    72781 ttttacgtgg tagccagact gcttcaagat tctttccgct agatagctac gtaatccgct
    72841 atgacagctg acgatatatt cttgttggct gtctaattca tttaatcggt cccgaagttc
    72901 attcaaaggg atatgtttgg cttccttgaa tttgcccttt tccaattctt ctgctgttcg
    72961 cacatctaat aaaatttttc cttttgctag ttctgtcggc aattcatgcc attgtacggt
    73021 ttcactaagc ccttctgcaa tgttcattgc tgcatatcct agcatgttta ctggatcttt
    73081 ggctgagcca aatggcggtg catacgtcaa ttctaattcc ggtaaatcga agatcgtcaa
    73141 atgccctttg attgctgttg ctaaaatatc gatccgttta tccacacctt ttgccccaac
    73201 accttgtgca ccataaatct cgccagtctc tggatggaag atcaacttca gtaaaatatc
    73261 tgttgcttct ggataatagc ttgcatgatc ttttcctgat acatgaatga cttcaaaaga
    73321 cagttcgttt tctttggctg ttctttcact taaaccggtg gatgcggcag tcattccaaa
    73381 cgttcgtata attgctgttc caataccgcc ttgatttctt cgagaaattc cggaaatcgt
    73441 gtccgcaact tgtctacctt gacgattggc tggtgaagca agagaaataa gtgcatcttt
    73501 tccagtgatt tgctgtttta caacgatagc atcccccacc gcataaatat caggaatatt
    73561 cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg cctaattcga ttccagcggt
    73621 cactgctagc tggttatcag gacgaacacc aatggataaa ataactaaat cagcagggat
    73681 ggtacgccca tcatccagtc gcaatcgttt cccgtggtct tcaaatccag cgacagcatg
    73741 agaagtaatc acttctactc cttgatgaga caattctttt tctataaaag ctgccatttc
    73801 ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc accatgactt ctaaacctct
    73861 tctttttagg ttttctgcca tttccaatcc gatgaatcct gcaccaacga tcacggcacg
    73921 ctttggctgt ttttctaatg catgcatcac tttatcgata tctggtacat ttctaataga
    73981 aaaaacattg tcggcttctg ttatccctgg aatcgatgga acaactggtt ttgcaccagg
    74041 agataaaacc aatgcatcgt actgttcaat gtgtttttga ccctcatgta cgacttctac
    74101 cgttttgttt tcgggaaaga tggctgtcac ttcatgattt ggacgcacat ctaaacaaaa
    74161 acgagcggat aatgattctg gcgtttggac aagaagattt tcccgttcac tgatttctcc
    74221 cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca tacggtcctt tttcaaaaac
    74281 aacgatttct gcatcttcca ttaatcggcg aagccgtgta gcagcagaca ttccacctgc
    74341 tacacctccg acaataacga ttttcatctt atttccctcc tactgttcga tcaaaccatt
    74401 gattcatgcc accacgtaca ttcactacat cataaccaga tttctttaat tctttagctg
    74461 cttgtttact cctcatccct gattgacaaa tcacatagac ggtcttttct ttgtctccat
    74521 catatctgtt tatactttgt aatggaacat ttttagctcc ttttatatgc ccgccccggt
    74581 attctgcagg tgttctaaca tcaagtaacg ctggattttc caaaagcttt tcctttaatt
    74641 ctgggactgt tatcgatttt attgattgaa atagcaaatt cattttctgc acctcattta
    74701 catttttata aatacctcat ggggtatatt aaacaggagc tttattaata tgtcaaccag
    74761 aatgactctt catttttttc actacttttt atatttgcat aaaataccga taaggggtat
    74821 tatctaatta taaaaataga ggagaatgtg ttatggagat tgataataaa aatatcctga
    74881 accgtttgaa acgtacagag ggacaaattc gtggtatcca aaaaatgatc gaagacgaaa
    74941 aagaatgtat ggatgtcatc actcaattaa gcgctgttcg ttctagtatt gatcgcgtaa
    75001 tgggcatgat cgttgcagat aatctgaaaa attgctttga gaaacctgat agtaatccag
    75061 aagaacaagc ggctaaacta gaacaagcga tcaagatgat tattaaaaag taaaggaatg
    75121 atttcaatga aagtcgtttt ccatattgat gaattagaaa aatggtcaga aacagggaaa
    75181 aacgtaaaaa acctgataaa agcttctcca gaaacgacga ttgtcgtatc tgtaaatggc
    75241 atcgcgatta ccggttatct agactctgcc aatgcagaat tcttagattt gcaaggagtc
    75301 gtttttcacg cttgcgccaa tgctatgcgt gctaatcata tttcagaaag ttctttacct
    75361 gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag ttgaactgca atcccaaggt
    75421 tatgcttata tcaagcctta gtcagtagag gttgcgacag aagtgatcag cttcaagaaa
    75481 tttcggctta tttttcgaag aagctacttc tggaaaaccg tgaatcaaag gttgtgaaaa
    75541 cgctgtcctg catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcgt
    75601 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    75661 aaatcagcgg tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt
    75721 gccgtttttt gagctaatac cgaaggtcag ctgattgaac accgtttaag gaagagattg
    75781 tgaaaaagtc gcttagtttc gagtattaag aaaaattttg tgaaaatgga tttctacatt
    75841 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt aaggaagagg
    75901 ttgtaaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agctgctcat
    75961 attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt ttaccaagag
    76021 gttgtgagcc taccatttaa ctccgaatca caagaaataa tttcaaaaac aacttctcct
    76081 gtttttggtt tggttggctt attatcgaga agtagctact gggagtctat ttatcaaaaa
    76141 aaggaatgcg acataggtca cacccctttt ttattttctt tgaaacatct ttcggtactc
    76201 gacagcatac aagacaaaag attgtaaaag accgtaagtg atgacggcaa aaatgactct
    76261 cacccacagc gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa catagatgac
    76321 agcagtcgct actttgccat accacttcgc tccacttaat ttgatatttt tagatagccc
    76381 gattaatcca gctacaaaca taaagccttc ttttatcaag aatagaccaa acaacagcca
    76441 aagccacggt cgataccatg ccatagacaa aatcagaaaa agttgtgtca atttatctgc
    76501 aaacggatca agcaccttcc ctaagtctgt tacctgatta tatctccgag caatgacccc
    76561 gtctagataa tcagacaaac ctgagacaac aatgaccgct cctgctatat aaaatgactg
    76621 tctcagcaca aaataaagat aaatcggcag caaaaaaata cggaatatcg ataaaagatt
    76681 cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat ttcattttat cgccctacct
    76741 cctcagcact cttttctttt tattttagct tacacgggga ataaaaccaa tgatttcact
    76801 gtttttattt ttctttacag ccactgatat tatgagctaa tttgagataa tcacttgctg
    76861 ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca tttcttttac tagtacttta
    76921 tgagcttttt ttgatcctcg ctcta
//
LOCUS       DACDSR010000004        70961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDSR010000004 DACDSR010000000
VERSION     DACDSR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.004"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IWK15_000282"
     CDS             162..392
                     /locus_tag="IWK15_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9545915.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IWK15_000283"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IWK15_000283"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9545916.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IWK15_000284"
     CDS             1803..1979
                     /locus_tag="IWK15_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9545917.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IWK15_000285"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IWK15_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9545918.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IWK15_000286"
     CDS             2474..2941
                     /locus_tag="IWK15_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9545919.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IWK15_000287"
     CDS             3026..3910
                     /locus_tag="IWK15_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9545920.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IWK15_000288"
     CDS             4027..4260
                     /locus_tag="IWK15_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9545921.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IWK15_000289"
     tRNA            4348..4428
                     /locus_tag="IWK15_000289"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IWK15_000290"
     tRNA            4435..4506
                     /locus_tag="IWK15_000290"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IWK15_000291"
     CDS             5021..5728
                     /locus_tag="IWK15_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9545922.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IWK15_000292"
     CDS             complement(5788..6432)
                     /locus_tag="IWK15_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9545923.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IWK15_000293"
     CDS             6559..6846
                     /locus_tag="IWK15_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545924.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IWK15_000294"
     CDS             6954..7274
                     /locus_tag="IWK15_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9545925.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IWK15_000295"
     CDS             7355..8668
                     /locus_tag="IWK15_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9545926.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IWK15_000296"
     CDS             8665..9384
                     /locus_tag="IWK15_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9545927.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IWK15_000297"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IWK15_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9545928.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IWK15_000298"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IWK15_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9545929.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IWK15_000299"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IWK15_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9545930.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IWK15_000300"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IWK15_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9545931.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IWK15_000301"
     CDS             14821..15714
                     /locus_tag="IWK15_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9545932.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IWK15_000302"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IWK15_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9545933.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IWK15_000303"
     CDS             17651..18508
                     /locus_tag="IWK15_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9545934.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IWK15_000304"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IWK15_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9545935.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IWK15_000305"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IWK15_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9545936.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IWK15_000306"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IWK15_000306"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9545937.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IWK15_000307"
     CDS             21264..22022
                     /locus_tag="IWK15_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9545938.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IWK15_000308"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IWK15_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9545939.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IWK15_000309"
     CDS             22950..23981
                     /locus_tag="IWK15_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9545940.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IWK15_000310"
     CDS             23978..24745
                     /locus_tag="IWK15_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9545941.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IWK15_000311"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IWK15_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9545942.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IWK15_000312"
     CDS             26713..27030
                     /locus_tag="IWK15_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9545943.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IWK15_000313"
     CDS             27030..27380
                     /locus_tag="IWK15_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9545944.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IWK15_000314"
     CDS             27547..28485
                     /locus_tag="IWK15_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9545945.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IWK15_000315"
     CDS             28500..29330
                     /locus_tag="IWK15_000315"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9545946.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IWK15_000316"
     CDS             29374..30399
                     /locus_tag="IWK15_000316"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9545947.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IWK15_000317"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IWK15_000317"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9545948.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IWK15_000318"
     CDS             31477..31917
                     /locus_tag="IWK15_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9545949.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IWK15_000319"
     CDS             31920..32522
                     /locus_tag="IWK15_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9545950.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32581..33687)
                     /locus_tag="IWK15_000320"
     CDS             complement(32581..33687)
                     /locus_tag="IWK15_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9545951.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IWK15_000321"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IWK15_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9545952.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35235..37580)
                     /locus_tag="IWK15_000322"
     CDS             complement(35235..37580)
                     /locus_tag="IWK15_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9545953.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37859..38761
                     /locus_tag="IWK15_000323"
     CDS             37859..38761
                     /locus_tag="IWK15_000323"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9545954.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38974..40749
                     /locus_tag="IWK15_000324"
     CDS             38974..40749
                     /locus_tag="IWK15_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9545955.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40815..42401)
                     /locus_tag="IWK15_000325"
     CDS             complement(40815..42401)
                     /locus_tag="IWK15_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9545956.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42652..42876
                     /locus_tag="IWK15_000326"
     CDS             42652..42876
                     /locus_tag="IWK15_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9545957.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43018..44769
                     /locus_tag="IWK15_000327"
     CDS             43018..44769
                     /locus_tag="IWK15_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9545958.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44769..46553
                     /locus_tag="IWK15_000328"
     CDS             44769..46553
                     /locus_tag="IWK15_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9545959.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46879..47730
                     /locus_tag="IWK15_000329"
     CDS             46879..47730
                     /locus_tag="IWK15_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9545960.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47784..49535
                     /locus_tag="IWK15_000330"
     CDS             47784..49535
                     /locus_tag="IWK15_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9545961.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49539..49709
                     /locus_tag="IWK15_000331"
     CDS             49539..49709
                     /locus_tag="IWK15_000331"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545962.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49749..50651)
                     /locus_tag="IWK15_000332"
     CDS             complement(49749..50651)
                     /locus_tag="IWK15_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9545963.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50882..52285
                     /locus_tag="IWK15_000333"
     CDS             50882..52285
                     /locus_tag="IWK15_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9545964.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52285..53718
                     /gene="ascB"
                     /locus_tag="IWK15_000334"
     CDS             52285..53718
                     /gene="ascB"
                     /locus_tag="IWK15_000334"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9545965.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53863..54789
                     /gene="trxB"
                     /locus_tag="IWK15_000335"
     CDS             53863..54789
                     /gene="trxB"
                     /locus_tag="IWK15_000335"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9545966.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55003..56730
                     /locus_tag="IWK15_000336"
     CDS             55003..56730
                     /locus_tag="IWK15_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9545967.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56802..57095
                     /locus_tag="IWK15_000337"
     CDS             56802..57095
                     /locus_tag="IWK15_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9545968.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57187..58047)
                     /locus_tag="IWK15_000338"
     CDS             complement(57187..58047)
                     /locus_tag="IWK15_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9545969.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58139..58567
                     /locus_tag="IWK15_000339"
     CDS             58139..58567
                     /locus_tag="IWK15_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9545970.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58729..58905
                     /locus_tag="IWK15_000340"
     CDS             58729..58905
                     /locus_tag="IWK15_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9545971.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58932..59378
                     /locus_tag="IWK15_000341"
     CDS             58932..59378
                     /locus_tag="IWK15_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9545972.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IWK15_000342"
     CDS             complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IWK15_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9545973.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61617..62588
                     /locus_tag="IWK15_000343"
     CDS             61617..62588
                     /locus_tag="IWK15_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9545974.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62612..64804
                     /locus_tag="IWK15_000344"
     CDS             62612..64804
                     /locus_tag="IWK15_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9545975.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64821..65297
                     /gene="ybeY"
                     /locus_tag="IWK15_000345"
     CDS             64821..65297
                     /gene="ybeY"
                     /locus_tag="IWK15_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9545976.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65275..65676
                     /locus_tag="IWK15_000346"
     CDS             65275..65676
                     /locus_tag="IWK15_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9545977.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65691..66590
                     /locus_tag="IWK15_000347"
     CDS             65691..66590
                     /locus_tag="IWK15_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9545978.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66733..67545
                     /gene="recO"
                     /locus_tag="IWK15_000348"
     CDS             66733..67545
                     /gene="recO"
                     /locus_tag="IWK15_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9545979.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            67929..68846
                     /gene="glyQ"
                     /locus_tag="IWK15_000349"
     CDS             67929..68846
                     /gene="glyQ"
                     /locus_tag="IWK15_000349"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9545980.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68848..70923
                     /locus_tag="IWK15_000350"
     CDS             68848..70923
                     /locus_tag="IWK15_000350"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9545981.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23222 a        12429 c        15179 g        20131 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32581 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32641 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32761 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    32941 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33001 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33181 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33301 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33361 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33421 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33481 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33541 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34081 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34141 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34201 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34261 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34501 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34561 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34681 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34741 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34801 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    34921 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    34981 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35041 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35101 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35161 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35221 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35281 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35341 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35401 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35461 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35521 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35581 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35641 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35701 acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35761 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35821 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    35881 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    35941 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36001 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36061 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36121 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36181 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36241 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36301 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36361 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36421 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36481 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36541 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36601 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36661 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36721 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36781 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36841 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    36901 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    36961 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37021 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37081 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37141 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37201 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37261 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37321 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37381 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37441 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37501 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37561 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37621 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37681 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37741 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37801 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37861 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    37921 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    37981 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38041 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38101 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38161 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38221 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38281 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38341 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38401 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38461 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38521 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38581 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38641 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38701 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38761 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38821 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    38881 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    38941 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39001 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39061 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39121 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39181 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39241 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39301 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39361 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39421 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39481 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39541 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39601 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39661 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39721 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39781 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39841 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    39901 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    39961 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40021 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40081 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40141 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40201 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40261 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40321 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40381 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40441 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40501 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40561 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40621 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40681 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40741 tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt
    40801 ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt
    40861 ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg
    40921 attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag
    40981 aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag
    41041 aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg
    41101 ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc
    41161 tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt
    41221 gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt
    41281 agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta
    41341 ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag
    41401 ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa
    41461 gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca
    41521 cccatgatca atagcagtgc attgactaga aaagccattg acaactggat attagaatcc
    41581 caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga
    41641 gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc
    41701 cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga
    41761 ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga
    41821 tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga
    41881 aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca
    41941 atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca
    42001 atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta
    42061 cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct
    42121 gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa
    42181 tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca
    42241 actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc
    42301 tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata
    42361 agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta
    42421 tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac
    42481 gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta
    42541 aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt
    42601 tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca
    42661 acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt
    42721 ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac
    42781 atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag
    42841 atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg
    42901 ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag
    42961 caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
    43021 tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
    43081 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
    43141 atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc
    43201 ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt
    43261 tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca
    43321 gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg
    43381 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
    43441 gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca
    43501 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa
    43561 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
    43621 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
    43681 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac
    43741 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
    43801 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
    43861 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
    43921 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc
    43981 gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc
    44041 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa
    44101 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa
    44161 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
    44221 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
    44281 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
    44341 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
    44401 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
    44461 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc
    44521 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
    44581 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
    44641 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
    44701 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
    44761 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
    44821 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg
    44881 ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc
    44941 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
    45001 ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt
    45061 atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
    45121 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
    45181 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
    45241 ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg
    45301 ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat
    45361 tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga
    45421 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
    45481 aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga
    45541 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt
    45601 tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
    45661 ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
    45721 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
    45781 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
    45841 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
    45901 ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca
    45961 tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
    46021 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
    46081 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
    46141 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
    46201 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
    46261 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
    46321 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc
    46381 gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
    46441 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
    46501 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt
    46561 tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt
    46621 tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt
    46681 cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata
    46741 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
    46801 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat
    46861 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc
    46921 agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa
    46981 gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg
    47041 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
    47101 tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat
    47161 attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta
    47221 ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa
    47281 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    47341 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
    47401 ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    47461 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    47521 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    47581 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    47641 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    47701 cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat
    47761 gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag
    47821 aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa
    47881 atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa
    47941 aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag
    48001 ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag
    48061 caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc
    48121 ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga
    48181 tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca
    48241 catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg
    48301 gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag
    48361 cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa
    48421 cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc
    48481 cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag
    48541 ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg
    48601 ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat
    48661 tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag
    48721 ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt
    48781 gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga
    48841 cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc
    48901 ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga
    48961 ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct
    49021 gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc
    49081 aaggtggact aggcatcttt acaattccca actatattag accagatggt gatttaacag
    49141 atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt
    49201 ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag
    49261 aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag
    49321 cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac
    49381 gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg
    49441 tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag
    49501 ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc
    49561 tacgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtgatcg
    49621 tcaccaatta cacggattat ttggatattt tggaaaacca aggggagacg gtcgggccag
    49681 acgatgcact tatcaccgca ctgacctgaa cggttaggtg aagaatggac taggcttaac
    49741 tattcttttt attccacttc tttattttca aaattgaaag gctctttcat gatggctgag
    49801 ctgatagggc gtttatcgaa tttttgaccg acagaaatta gatggactaa attctgttga
    49861 taattttctg caaaaacaca actatataac acgtctagaa gataacaaat tgatgtattg
    49921 atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct
    49981 gtactcattg cagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga
    50041 ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc
    50101 agaatcccgc acatatcact cgtgcaattg tacgcttcat agaatgattc accaaaagtg
    50161 gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc
    50221 aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg
    50281 gctttttgca gttggtcatg atgaatgaga gaaagtgtat catgaatcgt catctgttct
    50341 aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc
    50401 gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga
    50461 aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt agaggggtgg
    50521 acataacatc gctcagcaat cgcttggatg gtcatttcgc caagtgctgg agaatcgctt
    50581 aagagaaact ggatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca
    50641 cgtaataaca tgtagatacc ttcttcttat ttttttacgt aaattataac ttttttgaaa
    50701 atgatttgta aacggattta tactatataa cttaaaatag acattttttg acaagatatt
    50761 tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga aacagatttc
    50821 tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa
    50881 catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt
    50941 gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga
    51001 tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc gtatgtggga
    51061 tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag cattgaaaga
    51121 tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga
    51181 gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt
    51241 caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg
    51301 tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag aagcagttat
    51361 cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga tggcgatgat
    51421 gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct
    51481 aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca cggcagaatt
    51541 gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg atatcccgtt
    51601 tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta
    51661 tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg gtacaagagc
    51721 gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc cagaattaga
    51781 ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg ctgcctgtga
    51841 aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta caaagaatga
    51901 tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc
    51961 acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag gctggttgca
    52021 agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg attatgggac
    52081 agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag caaaacggat
    52141 catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg agacaatcag
    52201 ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat tatcaggtaa
    52261 tacacatata atggaggaag tataatggga ttcagaaaag attttttatg gggcggtgct
    52321 acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact agcaaacgta
    52381 gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa aaaaatgttt
    52441 gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta tcatcgttat
    52501 aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc
    52561 tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg cttgaaattt
    52621 tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt gacgattacg
    52681 cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc cagagaactc
    52741 gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa
    52801 tattggctga cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg
    52861 ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag
    52921 ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta
    52981 ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg
    53041 gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa
    53101 tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga aatggaacca
    53161 ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc ttattatgct
    53221 tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa tatctttgct
    53281 tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga tccattaggc
    53341 ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt tattgtagaa
    53401 aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga tgattaccga
    53461 atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact agacggagtc
    53521 gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg taccggtgag
    53581 atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa tggcacatta
    53641 gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc aaatggagaa
    53701 aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag cggatttttc
    53761 gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc
    53821 tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt
    53881 attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc
    53941 gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc agaaatcgaa
    54001 aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat gtatgaaagt
    54061 gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa ggatcacggc
    54121 agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt cattatcgca
    54181 acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgaggc
    54241 gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct agtagtcatc
    54301 gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta
    54361 gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt
    54421 gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa
    54481 atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca
    54541 gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt tagagaagca
    54601 ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac gaatatgcca
    54661 ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt
    54721 ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt agaagaaaca
    54781 acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt
    54841 cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa
    54901 tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct
    54961 atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg
    55021 tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat
    55081 ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact
    55141 gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga
    55201 caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga
    55261 ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc
    55321 aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca
    55381 gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct
    55441 cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct
    55501 cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc
    55561 gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa
    55621 gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa
    55681 acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg
    55741 ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct
    55801 gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt
    55861 gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct
    55921 gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact
    55981 gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc
    56041 actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca
    56101 attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct
    56161 gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa
    56221 agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt
    56281 ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg
    56341 caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc
    56401 ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg
    56461 acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca
    56521 gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca
    56581 ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt
    56641 tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa
    56701 gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa
    56761 actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat
    56821 caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa
    56881 ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa
    56941 tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt
    57001 ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt
    57061 tgaaccagac actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact
    57121 ggagtgtgac aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa
    57181 aaatgattaa tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc
    57241 taaagcattc aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac
    57301 tacacatcct agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc
    57361 gatatcggaa taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat
    57421 attatttaaa atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca
    57481 cagctgtttg ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt
    57541 atttgctaaa aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac
    57601 atctgcttct aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat
    57661 tgctttgatc cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat
    57721 tggcttgata ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact
    57781 aaataattga tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa
    57841 tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt
    57901 ttcatctgtc acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat
    57961 agtagcttgc gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt
    58021 aacgatttct gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt
    58081 attataccat actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat
    58141 gaaaaaacag acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata
    58201 tactaaaaaa agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc
    58261 taaagaagtt tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta
    58321 ccggaatctc catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga
    58381 aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc catcaccatc attttatctg
    58441 tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca
    58501 gctaggtgga tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg
    58561 tcaataagga tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg
    58621 acggtcaaaa cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt
    58681 tcaataacaa ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca
    58741 gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca
    58801 aaagcgggta ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga
    58861 cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg
    58921 gagttggtag tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag
    58981 cgaaagataa ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc
    59041 agattaaaga aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa
    59101 tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg
    59161 aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg
    59221 aagaaattcg acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag
    59281 aatttggcaa agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc
    59341 aagtgaatgc gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac
    59401 agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa
    59461 gaataagatt ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt
    59521 gtttgatttg gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga
    59581 taggcgtaat aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca
    59641 agtcctaatg cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca
    59701 gtttgctgac tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca
    59761 gaaaagaaca tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag
    59821 gaaagcaaga ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat
    59881 tggatgatat tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc
    59941 agccatgtca tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca
    60001 acgagtccca tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca
    60061 ccgaagttac ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat
    60121 aaaggcatct tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact
    60181 tctaacgcaa cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta
    60241 aaaccaagat cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca
    60301 tcaaccgtgt tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct
    60361 gcagcagatt ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct
    60421 aataatgcaa gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga
    60481 tgtacagtaa ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat
    60541 gaatcatatt tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc
    60601 cgcggtgtac caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca
    60661 taccattttc cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca
    60721 gctaagatag ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg
    60781 atccagtttg atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg
    60841 aagaacatcc cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt
    60901 gtttcttcca taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc
    60961 agaaactaga aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa
    61021 aaaagccctt ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc
    61081 gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata
    61141 atggaggcag ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat
    61201 tcgatttatt gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta
    61261 ctgttttcct cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt
    61321 tttgaaatgc aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc
    61381 cagagaatac ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat
    61441 gatcagcttt ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt
    61501 ttcttggtac ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt
    61561 cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga
    61621 cggaagagtc tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt
    61681 taggtgcaca cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata
    61741 cacgtggaga aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag
    61801 tcattcggac attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg
    61861 tgacggcact gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg
    61921 aagagatcgt caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga
    61981 aatatgtcga atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag
    62041 gaaaaacttt tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa
    62101 aaatcatttt gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg
    62161 atctaaaaga aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt
    62221 ttggattaga tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag
    62281 cttacatgcg ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca
    62341 cgacagttgc tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg
    62401 tcaatggaga tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg
    62461 cagaacgaac attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg
    62521 ttgttcggca ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc
    62581 aagaataagc tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca
    62641 agaaaaactt ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg
    62701 cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc
    62761 agaagaaagt atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag
    62821 aaaactggca gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga
    62881 ccagaacaat cggatcaagc aactatttga attgatcgat aaaacgaatg atgccatcaa
    62941 caaatcttat gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac
    63001 tacagaagag cggatcgccg cgttgaagaa aaattttgaa aatgtgaatg cagaaaatgt
    63061 tgcgttttat caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac
    63121 tgagatccat actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca
    63181 agttcgtgaa agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt
    63241 gaatgtcaca ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt
    63301 gaatgacgta ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt
    63361 acaaccagtg atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc
    63421 ttcagctatg aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc
    63481 gctaattgcc atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact
    63541 tcaattccaa aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc
    63601 agtcagcata gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat
    63661 cccgctgttt tatcccgctg cttttgcacc acttgtttta agctttttct tgaaccgaag
    63721 agcagggatc atggctggta ttttccaagc agtatctgca cttttcattt tttatgattc
    63781 gattggcacg aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc
    63841 aacgattgtc agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat
    63901 tatttttcct gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga
    63961 tgcaacgact tggttaatgc ttgtctgcgg gttcactggt gcactcttct cattcttgct
    64021 gacgatcggt ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt
    64081 aaatgaactc agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg
    64141 tacttatcat catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg
    64201 cggacgttct ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca
    64261 tgctagcttt ttcgttgaga acttgccttc aggagcggaa aacccgcaca atttcttatt
    64321 gccagaagac agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga
    64381 agattataat atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct
    64441 aatgcgttat ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt
    64501 ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag
    64561 ttgtgaggct gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt
    64621 ccataatctt atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt
    64681 gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt ttaagtagca ccttccattc
    64741 tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag ataaaggaag aacaagaaga
    64801 atagaaagaa gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag
    64861 aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc
    64921 cagaggaaac agaaatgtct gttactttca tggacaatgc ggcaatccaa gtaatcaatc
    64981 gtgattaccg tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag
    65041 gcgaagacga aatcccagtg atttttgaag atggagagaa tccattgcct cgtgtactag
    65101 gagacatcat gatttctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat
    65161 atgatcggga acttggcttt ttagcggtac atggattctt gcatatcaac gggtatgacc
    65221 atatgacacc agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct
    65281 atggactcaa aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt
    65341 gctgtccaag gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg
    65401 ttcggtatcc ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta
    65461 tggctgcttt tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa
    65521 aatgtagtgg acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat
    65581 atggcagctg gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta
    65641 tttgtcccta aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac
    65701 ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga
    65761 accgaatcgt gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata
    65821 aaatccaagg agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa
    65881 tccataaacc aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga
    65941 aagaagtgga tgcagtctta tttatgatca gtgctgatca aaaacgcggc cggggagacg
    66001 attttattat tgaacgtcta aaaaacgtgc attctccagt ttatctgatt atcaataaaa
    66061 tcgataagat ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc
    66121 cttttgcaca agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg
    66181 atgtattagt cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg
    66241 atcatcctga atactttatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac
    66301 gagacgaagt gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg
    66361 ataaaatcca tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta
    66421 tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa
    66481 atcttttagg agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag
    66541 acaaaaaagt ctatctgcaa gactttggct accgaaaaga cgaatactga tattctgaac
    66601 ttcttatagg ttaagtatag aaataaactg gataaaaatc cgcacgttga gaaaaacggc
    66661 ggaagatcga aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt
    66721 aggagggaga atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat
    66781 tacaaagaaa aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt
    66841 tatatcaaag gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc
    66901 gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa
    66961 gacgtccatg cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacctat
    67021 ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa
    67081 tttacgcaac aagctctgca gcggatggat caaggagatg atgcagaaat catcacgaat
    67141 atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc
    67201 gtatgcgggg aaacaaaagg aaaatttgac tattcttcca aatctggcgg tgtgatatgc
    67261 gagaaacact ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc
    67321 gttcgaatgt tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa
    67381 acgaagcagg caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat
    67441 ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca
    67501 gagaaaaata tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca
    67561 aaaaaatcaa aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa
    67621 atagatcgtt cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta
    67681 cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga
    67741 cccaaacgat ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat
    67801 tcggaagtag ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga
    67861 ctttttgtcg ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg
    67921 caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt
    67981 ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac agaaaaagga gcaggtacga
    68041 tgagcccata tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag
    68101 agccatcccg ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc
    68161 atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag
    68221 aaagcttgcg tcttttagga attgatccat tagaacacga tattcgtttt gttgaagaca
    68281 actgggagaa cccatcgatg gggtgtgccg gtgtgggttg ggaagtatgg cttgatggga
    68341 tggagatcac acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta
    68401 cttctgagat cacttatgga ctagaacgtc ttgcttcata tattcaagaa gttgaaagtg
    68461 tctatgatct ggaatgggct gatggtgtga aatatggcga gatcttcaaa caaccagaat
    68521 atgaacattc caaatattct tttgaagtaa gtgaccaaga cttgctacta agcaactttg
    68581 accgttttga aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg
    68641 attatatttt gaaatgcagc catacattca atttattaga tgctcgcggt gcagtatccg
    68701 taacagaacg agcaggttat ctttcccgta tccgcaacat ggctcgtgct gtagccaaaa
    68761 tctttgtggc agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc
    68821 cagaaaacat gaaggaggct gaataagatg gcaaaagact tattacttga aatcgggctg
    68881 gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac aattagaaga aaaaacagca
    68941 aaatttttag atgaaaacca attgacttat gatacgatcg aaacgttttc tacaccgcgc
    69001 cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag cagacagtga agaagaagtc
    69061 aaaggaccag ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagctgaa
    69121 ggatttgtcc gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc
    69181 gtggaatatg tctatgtaac gaaatacact cacggcaaag aagcgaaaga agtattgaca
    69241 ggtttagcag atgtcattaa aagcctcact ttcccagtaa cgatgcattg ggcagaatac
    69301 gattttgaat atatccgccc tattcactgg atcgtggcat tgttagatga cgaagtaatc
    69361 ccgtttgaga tcttagatgt caaaacaggc agagcctctc gaggacatcg tttcttaggc
    69421 gaagatgtga catttgcgca tgcaaaagaa tatgaagaaa aattgaatga acaattcgtt
    69481 attgcttcac cgcaaaaaag acaagaaatg atcgttacac aagcaaatga attagccgaa
    69541 aaaaatcact gggttattga tctagacgaa gatttactag aagaagtcaa caatttagtc
    69601 gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat atctttctgt tcctgaagaa
    69661 gtactagtca cttctatgaa agaacatcaa cgttatttcg aggtacggaa cgatcaaggt
    69721 atgctgcttc ctcattttat ttctgctcgt aatggtgatc gggtaaaatt ggaaaatgtg
    69781 gttcgaggca atcaaaaagt attgacggct cgtttagaag atgcggaatt cttttataat
    69841 gaagacaaga aagcttcaat cgaccaatac gttgaaaaac taaaaactgt tactttccat
    69901 gaaaaaatcg gtagtatcta tgagaaaatg cagcgggtac aagtcattgc ccgtatcatt
    69961 ggcgaacaag taggactatc tgaggaagaa ttgatggatc tgcaacgagc tgctgaaatc
    70021 tataaatttg atctagtaac caacatggta ggggaatttc ctgaactaca agggatcatg
    70081 ggcgaaaaat atgctctgct gaaaggcgag aaacctgctg tagcaacagc gattcgtgag
    70141 cattacatgc ctatatccag tgaaggagag ctacctcagt ctacagtagg ttctgtttta
    70201 gcgattgcag ataaattaga tagcattttt tctttctttg cagttgggat gattccaagc
    70261 gggtcaaacg atccttatgc cttacgtcgc caagcatacg gtgtcgttcg gatcatcgaa
    70321 tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga tgttatcagt
    70381 aaagatacgg atcgtttcgg cattggttta tcagaagggc aacaacaggt gatcgacttt
    70441 atcaaaggcc gtctgcgcca attattgaca acgaagaata ttcgtcatga tgtcatcgaa
    70501 gctgttttga atgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc
    70561 aagcagcatc ttgcagatga agacttcaaa ccatcgatgg aagctttgac acgtgtcgtg
    70621 aacttagcga aaaaagcgga gcttgaaaaa caatcagaag tagaccctga actgttcgaa
    70681 aatgaagcgg agaaagaact gcacaaagca gtagaggcaa tgaaaaaaag ctttgctgac
    70741 caaacgatca acgaaaatta tgatcagctt gtaagccttc gcccactgat cgaaaattat
    70801 tttgatcaga cgatggtcat ggcagaagaa ccagcagtac gtaagaaccg attaacgcaa
    70861 ttaagtcaaa ttgctgccat ggctttgtcg ctagcaagtt tagatcaaat catcacaaaa
    70921 taatttctaa tatgtaaata aaccagagaa taggcacaaa a
//
LOCUS       DACDSR010000005        65091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDSR010000005 DACDSR010000000
VERSION     DACDSR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.005"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(23..1483)
                     /locus_tag="IWK15_000351"
     CDS             complement(23..1483)
                     /locus_tag="IWK15_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9545982.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(1473..2768)
                     /locus_tag="IWK15_000352"
     CDS             complement(1473..2768)
                     /locus_tag="IWK15_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9545983.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(3100..3777)
                     /locus_tag="IWK15_000353"
     CDS             complement(3100..3777)
                     /locus_tag="IWK15_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9545984.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(3807..4898)
                     /locus_tag="IWK15_000354"
     CDS             complement(3807..4898)
                     /locus_tag="IWK15_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9545985.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(4999..5433)
                     /locus_tag="IWK15_000355"
     CDS             complement(4999..5433)
                     /locus_tag="IWK15_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9545986.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(6017..7474)
                     /gene="ascB"
                     /locus_tag="IWK15_000356"
     CDS             complement(6017..7474)
                     /gene="ascB"
                     /locus_tag="IWK15_000356"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9545987.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(7471..9366)
                     /locus_tag="IWK15_000357"
     CDS             complement(7471..9366)
                     /locus_tag="IWK15_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9545988.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(9424..11199)
                     /locus_tag="IWK15_000358"
     CDS             complement(9424..11199)
                     /locus_tag="IWK15_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9545989.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(11249..12745)
                     /locus_tag="IWK15_000359"
     CDS             complement(11249..12745)
                     /locus_tag="IWK15_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9545990.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(12939..14561)
                     /locus_tag="IWK15_000360"
     CDS             complement(12939..14561)
                     /locus_tag="IWK15_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9545991.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(15010..16323)
                     /locus_tag="IWK15_000361"
     CDS             complement(15010..16323)
                     /locus_tag="IWK15_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9545992.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(16568..16792)
                     /locus_tag="IWK15_000362"
     CDS             complement(16568..16792)
                     /locus_tag="IWK15_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545993.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(16812..17180)
                     /locus_tag="IWK15_000363"
     CDS             complement(16812..17180)
                     /locus_tag="IWK15_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9545994.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(17378..18088)
                     /locus_tag="IWK15_000364"
     CDS             complement(17378..18088)
                     /locus_tag="IWK15_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9545995.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(18106..18603)
                     /locus_tag="IWK15_000365"
     CDS             complement(18106..18603)
                     /locus_tag="IWK15_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9545996.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(18640..19092)
                     /locus_tag="IWK15_000366"
     CDS             complement(18640..19092)
                     /locus_tag="IWK15_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9545997.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(19227..19832)
                     /locus_tag="IWK15_000367"
     CDS             complement(19227..19832)
                     /locus_tag="IWK15_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9545998.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(20282..20902)
                     /locus_tag="IWK15_000368"
     CDS             complement(20282..20902)
                     /locus_tag="IWK15_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9545999.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(20904..21773)
                     /locus_tag="IWK15_000369"
     CDS             complement(20904..21773)
                     /locus_tag="IWK15_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9546000.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(21796..22203)
                     /locus_tag="IWK15_000370"
     CDS             complement(21796..22203)
                     /locus_tag="IWK15_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9546001.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(22222..23511)
                     /locus_tag="IWK15_000371"
     CDS             complement(22222..23511)
                     /locus_tag="IWK15_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546002.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(23538..23840)
                     /locus_tag="IWK15_000372"
     CDS             complement(23538..23840)
                     /locus_tag="IWK15_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9546003.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(23865..25958)
                     /locus_tag="IWK15_000373"
     CDS             complement(23865..25958)
                     /locus_tag="IWK15_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9546004.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(26447..27211)
                     /locus_tag="IWK15_000374"
     CDS             complement(26447..27211)
                     /locus_tag="IWK15_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9546005.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(27322..28191)
                     /gene="mdcB"
                     /locus_tag="IWK15_000375"
     CDS             complement(27322..28191)
                     /gene="mdcB"
                     /locus_tag="IWK15_000375"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9546006.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(28216..29292)
                     /locus_tag="IWK15_000376"
     CDS             complement(28216..29292)
                     /locus_tag="IWK15_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9546007.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(29316..29909)
                     /locus_tag="IWK15_000377"
     CDS             complement(29316..29909)
                     /locus_tag="IWK15_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9546008.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(29913..31589)
                     /gene="mdcD"
                     /locus_tag="IWK15_000378"
     CDS             complement(29913..31589)
                     /gene="mdcD"
                     /locus_tag="IWK15_000378"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9546009.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(31579..31878)
                     /locus_tag="IWK15_000379"
     CDS             complement(31579..31878)
                     /locus_tag="IWK15_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9546010.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(31980..33626)
                     /gene="mdcA"
                     /locus_tag="IWK15_000380"
     CDS             complement(31980..33626)
                     /gene="mdcA"
                     /locus_tag="IWK15_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9546011.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(33633..34544)
                     /locus_tag="IWK15_000381"
     CDS             complement(33633..34544)
                     /locus_tag="IWK15_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9546012.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            34664..35521
                     /locus_tag="IWK15_000382"
     CDS             34664..35521
                     /locus_tag="IWK15_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9546013.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(35708..36070)
                     /locus_tag="IWK15_000383"
     CDS             complement(35708..36070)
                     /locus_tag="IWK15_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546014.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            36416..37855
                     /locus_tag="IWK15_000384"
     CDS             36416..37855
                     /locus_tag="IWK15_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9546015.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(37862..40414)
                     /locus_tag="IWK15_000385"
     CDS             complement(37862..40414)
                     /locus_tag="IWK15_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9546016.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(40528..41859)
                     /locus_tag="IWK15_000386"
     CDS             complement(40528..41859)
                     /locus_tag="IWK15_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9546017.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(41825..43303)
                     /locus_tag="IWK15_000387"
     CDS             complement(41825..43303)
                     /locus_tag="IWK15_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9546018.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(43608..45443)
                     /gene="tdc"
                     /locus_tag="IWK15_000388"
     CDS             complement(43608..45443)
                     /gene="tdc"
                     /locus_tag="IWK15_000388"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9546019.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            45913..47340
                     /locus_tag="IWK15_000389"
     CDS             45913..47340
                     /locus_tag="IWK15_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9546020.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(47432..47797)
                     /locus_tag="IWK15_000390"
     CDS             complement(47432..47797)
                     /locus_tag="IWK15_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9546021.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(47794..48159)
                     /locus_tag="IWK15_000391"
     CDS             complement(47794..48159)
                     /locus_tag="IWK15_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9546022.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(48351..48599)
                     /locus_tag="IWK15_000392"
     CDS             complement(48351..48599)
                     /locus_tag="IWK15_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546023.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            48598..48726
                     /locus_tag="IWK15_000393"
     CDS             48598..48726
                     /locus_tag="IWK15_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9546024.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            48783..50477
                     /locus_tag="IWK15_000394"
     CDS             48783..50477
                     /locus_tag="IWK15_000394"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9546025.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            50577..50750
                     /locus_tag="IWK15_000395"
     CDS             50577..50750
                     /locus_tag="IWK15_000395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546026.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            51128..52261
                     /locus_tag="IWK15_000396"
     CDS             51128..52261
                     /locus_tag="IWK15_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9546027.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            52292..52612
                     /locus_tag="IWK15_000397"
     CDS             52292..52612
                     /locus_tag="IWK15_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546028.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(52641..53105)
                     /locus_tag="IWK15_000398"
     CDS             complement(52641..53105)
                     /locus_tag="IWK15_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546029.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(53237..54490)
                     /locus_tag="IWK15_000399"
     CDS             complement(53237..54490)
                     /locus_tag="IWK15_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546030.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(54519..54836)
                     /locus_tag="IWK15_000400"
     CDS             complement(54519..54836)
                     /locus_tag="IWK15_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9546031.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(54862..55182)
                     /locus_tag="IWK15_000401"
     CDS             complement(54862..55182)
                     /locus_tag="IWK15_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9546032.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(55646..57061)
                     /locus_tag="IWK15_000402"
     CDS             complement(55646..57061)
                     /locus_tag="IWK15_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9546033.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(57078..58394)
                     /locus_tag="IWK15_000403"
     CDS             complement(57078..58394)
                     /locus_tag="IWK15_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546034.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(58544..59242)
                     /locus_tag="IWK15_000404"
     CDS             complement(58544..59242)
                     /locus_tag="IWK15_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9546035.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(59531..60520)
                     /locus_tag="IWK15_000405"
     CDS             complement(59531..60520)
                     /locus_tag="IWK15_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9546036.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(60600..61433)
                     /gene="msrA"
                     /locus_tag="IWK15_000406"
     CDS             complement(60600..61433)
                     /gene="msrA"
                     /locus_tag="IWK15_000406"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9546037.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(61608..63242)
                     /locus_tag="IWK15_000407"
     CDS             complement(61608..63242)
                     /locus_tag="IWK15_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546038.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(63229..65076)
                     /locus_tag="IWK15_000408"
     CDS             complement(63229..65076)
                     /locus_tag="IWK15_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546039.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        19442 a        13501 c        10843 g        21305 t
ORIGIN      
        1 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
       61 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
      121 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
      181 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
      241 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
      301 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
      361 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
      421 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
      481 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
      541 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
      601 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
      661 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
      721 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
      781 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
      841 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
      901 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
      961 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
     1021 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
     1081 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
     1141 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
     1201 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
     1261 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
     1321 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
     1381 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
     1441 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
     1501 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
     1561 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
     1621 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
     1681 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
     1741 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
     1801 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
     1861 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
     1921 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
     1981 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
     2041 tcttcccaca tctgattgtc ggggcgagaa tgattagcca gcaaaacaag atcatagttc
     2101 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
     2161 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
     2221 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
     2281 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
     2341 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
     2401 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
     2461 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
     2521 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
     2581 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
     2641 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
     2701 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
     2761 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
     2821 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
     2881 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
     2941 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
     3001 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
     3061 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
     3121 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
     3181 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
     3241 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
     3301 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
     3361 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
     3421 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
     3481 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
     3541 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
     3601 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
     3661 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
     3721 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
     3781 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
     3841 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
     3901 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
     3961 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
     4021 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
     4081 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
     4141 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
     4201 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
     4261 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
     4321 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
     4381 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
     4441 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
     4501 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
     4561 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
     4621 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
     4681 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
     4741 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
     4801 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
     4861 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
     4921 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
     4981 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
     5041 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
     5101 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
     5161 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
     5221 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
     5281 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
     5341 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
     5401 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
     5461 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
     5521 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
     5581 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
     5641 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
     5701 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
     5761 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
     5821 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
     5881 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
     5941 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
     6001 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
     6061 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
     6121 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
     6181 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
     6241 tatgctgttt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
     6301 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
     6361 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
     6421 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
     6481 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
     6541 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
     6601 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
     6661 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
     6721 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
     6781 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
     6841 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
     6901 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
     6961 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
     7021 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
     7081 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
     7141 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
     7201 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
     7261 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
     7321 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
     7381 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
     7441 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
     7501 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
     7561 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
     7621 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
     7681 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
     7741 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacaggact
     7801 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
     7861 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
     7921 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
     7981 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
     8041 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
     8101 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
     8161 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
     8221 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
     8281 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
     8341 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
     8401 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
     8461 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaagt
     8521 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
     8581 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
     8641 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
     8701 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
     8761 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
     8821 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
     8881 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
     8941 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
     9001 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
     9061 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
     9121 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
     9181 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
     9241 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
     9301 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
     9361 ctccattcaa ttagtacccc ttataatagc gaacgaaact gtacaatagt gtgtatggtt
     9421 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
     9481 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
     9541 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
     9601 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
     9661 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
     9721 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
     9781 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
     9841 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
     9901 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
     9961 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    10021 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    10081 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    10141 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    10201 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    10261 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    10321 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    10381 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    10441 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    10501 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    10561 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    10621 aattgtactg atttgttgag gcaaccttgc gtgttggatc accgtttctg ttccaacaag
    10681 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    10741 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    10801 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    10861 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    10921 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    10981 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    11041 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    11101 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    11161 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    11221 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    11281 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    11341 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    11401 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    11461 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    11521 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    11581 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    11641 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    11701 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    11761 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    11821 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    11881 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    11941 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    12001 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    12061 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    12121 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    12181 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    12241 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    12301 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    12361 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    12421 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    12481 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    12541 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    12601 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    12661 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    12721 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    12781 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    12841 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    12901 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    12961 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    13021 acaataaccc ttaaagcttc acgtggaaca gtgaacgtct gcttttcact ggacatatta
    13081 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    13141 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    13201 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    13261 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    13321 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc attgattgat
    13381 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    13441 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    13501 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    13561 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    13621 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    13681 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    13741 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    13801 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    13861 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    13921 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    13981 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    14041 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    14101 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    14161 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    14221 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    14281 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    14341 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    14401 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    14461 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    14521 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    14581 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    14641 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    14701 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    14761 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    14821 aatatgagga actatttttt cgtttttttc tctaatactc aggccagaac actgatttca
    14881 caacctcttt ataaacggtg ttcactcagc tgtccctcgg tattagctca aaaaacggca
    14941 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    15001 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    15061 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    15121 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    15181 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    15241 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    15301 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    15361 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    15421 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    15481 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    15541 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    15601 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    15661 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    15721 tatcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    15781 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    15841 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    15901 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    15961 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    16021 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    16081 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc
    16141 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    16201 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    16261 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    16321 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    16381 taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg
    16441 attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa
    16501 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
    16561 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
    16621 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
    16681 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
    16741 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
    16801 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
    16861 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
    16921 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
    16981 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
    17041 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
    17101 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
    17161 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
    17221 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
    17281 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
    17341 aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat
    17401 ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca
    17461 acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca
    17521 gcaccgctgc ttgtgtttct tccgtcagca ttgttctttg ggcttcacgc cagttgagac
    17581 agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat
    17641 cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat
    17701 ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac
    17761 tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt
    17821 tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct
    17881 gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg
    17941 cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt
    18001 ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga
    18061 aattggtatc cactattact tttttcatct gttcacacct cttgattaga ataattgctt
    18121 gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt
    18181 gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag
    18241 ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt
    18301 accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg
    18361 atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc
    18421 ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt
    18481 ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc
    18541 aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt
    18601 catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc
    18661 ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta aaagatattc
    18721 caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt
    18781 ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat
    18841 ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat
    18901 tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat
    18961 tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc
    19021 tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag
    19081 gatttcagcc atttcatccc acctttattt tgtttgtaag actagtttac ctctttttgt
    19141 tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat
    19201 attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa
    19261 ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta
    19321 aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc
    19381 gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc
    19441 gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact
    19501 tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac
    19561 cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca
    19621 cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct
    19681 tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca
    19741 aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt
    19801 agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta
    19861 tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc
    19921 tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg
    19981 ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa
    20041 atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac
    20101 aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat
    20161 caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc
    20221 tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt
    20281 gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt
    20341 gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc
    20401 ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat
    20461 cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt
    20521 cacgatcacg tttgtaccga cgttgacata acacttcaaa gtctgctgtt aggcgaatcg
    20581 tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac
    20641 taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt
    20701 tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa
    20761 acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg
    20821 gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca
    20881 gaagaatgaa atactttttc atctcattta tttcctaagt taattggact ttccaagttc
    20941 ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca
    21001 ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc
    21061 cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca
    21121 ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac atcaactact
    21181 gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga
    21241 ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg
    21301 tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg
    21361 ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact
    21421 gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa
    21481 gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc
    21541 aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc
    21601 atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct
    21661 tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact
    21721 gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat
    21781 tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat
    21841 ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc
    21901 tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt
    21961 attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa
    22021 tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat
    22081 ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc
    22141 tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa
    22201 catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata
    22261 taagagtgct gcggctactg tatctacatc ggttgctgta gcattgatga atcccatcga
    22321 attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc
    22381 gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc
    22441 ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt
    22501 aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg
    22561 gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat
    22621 agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa
    22681 tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg
    22741 atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag
    22801 cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg
    22861 caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac
    22921 accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc
    22981 agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc
    23041 gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac
    23101 tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt
    23161 tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc
    23221 tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat
    23281 cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat
    23341 gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat
    23401 gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt
    23461 gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc
    23521 ctcttttctt tttgtattta gataactccc ttatcttgca gaactggggt gattttttct
    23581 tcaatttcat tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact
    23641 gcgaactttt cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg
    23701 gaaactgcgg aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa
    23761 acatcctcag cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat
    23821 aaaatgttca atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact
    23881 gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc
    23941 tcttcgttgt tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct
    24001 actgctgcca aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt
    24061 ggttgctcaa tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca
    24121 tgggctagtg ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga
    24181 atcatcgctt cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct
    24241 agttcaatcg attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc
    24301 aaaatatctt ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg
    24361 aataattttt ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg
    24421 acatctatta tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt
    24481 agttcttcat ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc
    24541 ggatcgacaa ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga
    24601 ttgacatcca atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg
    24661 atctgaggaa acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg
    24721 ttacagacaa cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc
    24781 gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact
    24841 tcttcgatcg taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg
    24901 atgttttctt tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca
    24961 gccatgtgct tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt
    25021 gtctcgttct ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg
    25081 attgaattgg agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt
    25141 tctacttgcg ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa
    25201 aagctactgt cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc
    25261 ctgattcgct ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct
    25321 tcttgtttca acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga
    25381 aaataatgga gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag
    25441 tcggtcattc ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct
    25501 gaacctttat aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc
    25561 agtgttgctc gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct
    25621 tcttcttttt tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca
    25681 aaaatacgtt cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat
    25741 cgagcttgct tttccttttc cagcttcaac aaaaaaactt tgttgcgaat tgtacggatc
    25801 tccggaagct ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc
    25861 cgctgactga cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc
    25921 atgattctgt gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt
    25981 caagtatatg gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa
    26041 gaaagtaagc aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa
    26101 gacatacgcc tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa
    26161 gctgtcctgc atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt
    26221 tggttgttct tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga
    26281 cagagatgtt cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta
    26341 caaaatcgtg ccttattgcc gataggctat ctctggcaca ctgtttattc agaaaaagga
    26401 gctgcgacaa ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa
    26461 tcggctgaat aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata
    26521 tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat
    26581 cgactgaata atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct
    26641 gtcgataatt tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg
    26701 ctttgttttt ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc
    26761 tggctttttc aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga
    26821 tttccagaaa ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg
    26881 ctttcagcct tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta
    26941 ctgacaaaac agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg
    27001 gaccataata cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat
    27061 atgaacgacc accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat
    27121 gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca
    27181 aaatgattgc taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa
    27241 aactattttt atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
    27301 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc
    27361 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    27421 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    27481 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    27541 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    27601 ttgtttctgt acatatgaat cattatctag ctccttagct gcttggattg caagcctgat
    27661 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    27721 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    27781 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    27841 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    27901 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    27961 ctcctgatca atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    28021 cgtcttagca gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag
    28081 tgcatccact aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac
    28141 caacgcagcg agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac
    28201 tccttcttcc tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag
    28261 taaccagctt aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac
    28321 tcctgaaggt aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa
    28381 tacattggct aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt
    28441 tggcgaatcg tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa
    28501 actgtataaa ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg
    28561 atgtgcgagc aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag
    28621 ataacgtact ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc
    28681 ataaatcatg aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc
    28741 agtcaatact tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat
    28801 caatactggc catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa
    28861 cggcatgaac aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc
    28921 acctgtgaca tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac
    28981 tgctggaaat agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa
    29041 aaacttttct ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata
    29101 aacaattgca aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag
    29161 aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat
    29221 cataacagcc gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt
    29281 gttttcaaac atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta
    29341 ggtccttcga ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt
    29401 gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc
    29461 gattgaatta cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa
    29521 tcactggttt cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg
    29581 attccccatt tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga
    29641 taatccatga ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg
    29701 acttgtttga cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca
    29761 cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac
    29821 atcttttcag ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca
    29881 aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg
    29941 gcacggactt tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga
    30001 tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg
    30061 atgactttgc tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga
    30121 tagatcgctc ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca
    30181 gcagctgctt cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac
    30241 atcgcttgga cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca
    30301 tgtgctaaaa atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca
    30361 gcttgacgga gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc
    30421 aattcttcgt tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt
    30481 ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa
    30541 ccttcaacta aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt
    30601 acgattgatg tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta
    30661 ctaatagaag aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca
    30721 cttgctgcag ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga
    30781 tggttagcaa ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa
    30841 tcaagtaatg ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc
    30901 attgctgcga tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg
    30961 atttttgatt ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta
    31021 gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact
    31081 ctcgcttttt ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca
    31141 aatgatccta cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg
    31201 attacatttc cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca
    31261 ccacctgtat ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac
    31321 acatgttcaa gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg
    31381 aattttcctt ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca
    31441 acgatcccgt catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt
    31501 tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg
    31561 gcgtttaatt ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg
    31621 taggtacaca actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg
    31681 ataacgatca aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc
    31741 tgtacaaatt tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc
    31801 tcctgaaccg accactccta catggatttt cttattgatc ggctgtttcg ttggatattt
    31861 ataagctaat tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc
    31921 ttattcgtct ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt
    31981 tatgaccaag ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa
    32041 tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga
    32101 tcttctggta atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta
    32161 gtcattccaa gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt
    32221 gtttttgctt tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg
    32281 ctataaatca tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct
    32341 aattcttcga caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact
    32401 aatttttggc ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta
    32461 gaatgacgac gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa
    32521 cgtccttttg ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata
    32581 aacatatcga tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg
    32641 ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact
    32701 tcgccaccaa aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt
    32761 ggatgtgggt tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca
    32821 taagttggca gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat
    32881 tgtacgccgt gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat
    32941 tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct
    33001 ttcacgacaa cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact
    33061 tcgttgactt gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt
    33121 gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct
    33181 actaaaacaa catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta
    33241 tggatatcac ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt
    33301 gcaccagcat aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt
    33361 tctggtcgtg agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact
    33421 ttttcaagac tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact
    33481 ttgtcgccgg gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac
    33541 ttgccattga gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa
    33601 gcccagttgc ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg
    33661 gcgtcatcaa taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat
    33721 tttgtatgaa tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt
    33781 cctaattctt ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc
    33841 aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata
    33901 tacaaacatc tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc
    33961 gtatcattca tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc
    34021 gcttggtgcc gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac
    34081 gtatgttgca tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt
    34141 tttgctacaa tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa
    34201 ccgcttggat aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc
    34261 tcaaatctca aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct
    34321 gcaatatcag gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc
    34381 agtaatgcta gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga
    34441 taccccgtca cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct
    34501 aacttctgac tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca
    34561 gcacttactt tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa
    34621 aagaaaaaac cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct
    34681 gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt
    34741 tatttctcaa cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc
    34801 tttgttttac catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc
    34861 atatgcagtc aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa
    34921 tgagaagatc gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa
    34981 cttggtccga atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca
    35041 tttaccagat gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat
    35101 gaccaatatt cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct
    35161 tgctaaaaaa gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc
    35221 aaagagcaac gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt
    35281 gacgatcagt tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt
    35341 agctgttagc tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt
    35401 gcgggaaatc aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga
    35461 agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata
    35521 aaaaaaaaag agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc
    35581 cagaagtaat ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg
    35641 taaattcctt cttatttctt gaagctaaac atttctgtca caacctcttc tctattgtcc
    35701 tagacattca ttgcgttgat ttaaaacttc catcaatcaa atctgctagt tcatctagct
    35761 tttctatttt acgtagatca atagagatcc aatgttcttt attcatgtga taggctggaa
    35821 aatacccctc ttttcctcgc aaaattccgc caagttcagg atcgaccttc aaatcaagaa
    35881 tattgatgat cctatggtca cttgcatcat catagatttt ttcttctgga acagacatca
    35941 ggaccccaaa ccattttttg cttttttgat gtctgaatac gcaataatca ggaaattttt
    36001 taaaaatata ttctggagac aaattgtatg tttcattgat gtgatcgatt agttcttttt
    36061 tcgttagcat ctgcttccat ctctttctta ttgattcgct ttattcaaag ataccacgta
    36121 agaatagaaa aatgaataca agtagttgct aatcaaatac ttcgatattt ttctttttta
    36181 tcaactctta aatgttcaat ccgcctgtgt tggtcttgct accatctact attaaaatgt
    36241 ttattattgt ttgcccacct attttttgat tttagtggta tcatttttca agttcatgat
    36301 gaaattctta tcatcagtaa atgctccaga aaaataaaca atgattgaaa aatcagttga
    36361 aacttgacca ccgtttattt tctccgtatt cttagaatag aagggagtct gattgatgta
    36421 ttcactaatg aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt ctttagtaga
    36481 acaattgctg aatcatacaa agataaccgt aaatgaatta gctgaaatca tagggacaac
    36541 cgaacgaacg attttttctg accttcaatc tattcgctct cagctgcccg aaggctggga
    36601 catcatcagc gatcaagcag gaatcagttt gcaaaaccag caaaatttat taacaaatga
    36661 cctttgggag atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt
    36721 tacaaaaaaa gttgctgtcc ctgattttct agccgaccat gggctttctt atgggacatt
    36781 gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc agatcgattt
    36841 gacgaaatat acagcttgta ttcttggaaa agagcgtgcg attcgtactt tttatcatcg
    36901 attgctgatt cctttcactc ataacaacta ttttttcgag gattattcga tccatgaatc
    36961 ccattatttt caatttttaa gaaacctcag tcaaactgaa ctagcggttg aaacagaaga
    37021 gattttcgga acatgttggt tctttatcaa tacgatccgt ataaaagcga attgccgatt
    37081 agattcttct atccatacca acagcacact ctcctccctg tatgactctg cattgaaaaa
    37141 attgtattta acggaaggta tttatcttaa agacactgag ctttcttttg cttctttctg
    37201 ctttctggaa agttggaatt acaacaataa ctatggacaa gaaaccgctc gctgcctgca
    37261 tcattctcca tttttagagg tcctcgaaac gtttgtagag gaactagctt cagagctgtc
    37321 tcttgatcaa ctaaaaaaaa catctctgac tgataacttg gctctcctca tattaaaata
    37381 tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac
    37441 atatgatcag caatatgcac acttgtatca acagaaagag aatttactga acgatctaaa
    37501 aaaagaaata gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc tactgatcca
    37561 acaaacgatt ttttctatca aaccgaattt gttcaatgtc tattttattt tccaaggaga
    37621 accttcatgg aaggcgttct tgcagcagga attaaaagat tacttaggaa aacgtgtctg
    37681 cttcttacct atcgaactaa ccgatctttc cactatttct tttgaaaaaa cagatatctt
    37741 gatttccaat ttccctctag attctctaga tatccctatt gtatacattt cttctattcc
    37801 aacgaaaaat gagctcaatc gtttgactga actcactttc aaaacttttt tatagtaacg
    37861 gctagcgact gtattctcta gctgtttttt cttttttgcg aaacagtttg ttcatgatcc
    37921 ctttttctat ttctacaact aagaatagaa gaatcacatt tgctccaatc aataactgtt
    37981 gtgtcatcgt taaacttact gtaccgatca gctgctggcc gactggtaga aaaataacca
    38041 tcccttgcaa taaagcaagg atacctaatg aaagaaaaag cgctttgttt tgcaaaagac
    38101 cttggttcaa tgacggcttg gacaactctc tgcaattgat catgtaaaca gcttgagcta
    38161 agacgatatt ttgcagaaga atggtttgct gtaagctttg cccttcaaac tgcatagcca
    38221 ataaataggc aggaaccatg atcagtaggg agacatagat gatccgaaac aaatgataac
    38281 cagataggat tccttgcttt ggatctcttg gtgggcgctt catgatttct ggatctggcg
    38341 actcgaaccc tagtgcataa gataaagtga ttgtcgttac catattgacc cataaaatct
    38401 gtactgggga caaaggtagc gggcgattca gcataagtgc ccaaatcaag atcaatcctt
    38461 gagctaatgc agttggcaag aagaaattga tggttttttg caaattatcc aaaatccgcc
    38521 ggccctcttt tacggctttt gcaatcgtat ggaaattatc atctcctaaa accatatcag
    38581 ctgcctgttt gcttacttca ctacctttga ttcccatagc aattcctaca tcggatcttt
    38641 tcaaagcagg tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct
    38701 gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta
    38761 ttggcacttg ctgaatcaac tcttcatcag acataagatc caaatcgatc ccttctaaaa
    38821 ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat
    38881 cccctgtaat cattttcact gagatccctg cttgttggca ttcctttaca gctttgactg
    38941 cactttcttt tggcggatcg atcaatccgg cgatccctgc aaaagttaat tgggttaaag
    39001 tctcatgcgt gagttcctgt ttcgaatcta cttttttata agcaaatcct aaaacacgct
    39061 gaccttttct cgcaaattct gcagctgtct gactccatgc ttcttgttct gcagaagaaa
    39121 gagttgattt agcaaataat acttctggtg cacctttgac gtaaatcacc gctccttttt
    39181 cctccgcatg catcgttgcc atatatttgt attcagaact gaacggaatc tttgcttcta
    39241 tttttttcaa aggtagattg gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat
    39301 tgccacttaa attttctgtt ttctgttctt cttttgtttt taattcctga cagtttttca
    39361 taatggacaa cacgcaggct tcttcttttg cagctacatc cacaactgtc atttcatttt
    39421 ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat agaacctaat gtttctaccg
    39481 aaggaagtcc tttgatgatc gccttttctt ttgccatttc atgtactcca acggatagaa
    39541 tcatggtcaa aacagctggc aatccttcgg gtaccattga caccaccaaa gcaatcgtag
    39601 ccgaaagaag gatatgccaa tccataccat atcgaaatgt cgtaaaaata actaagaata
    39661 ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt tttttaatca
    39721 gaggcgtcgt ttgttgttcg accgattgca atgcttgatt gattttaccg atttctgtcg
    39781 tgtttcctgt ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa
    39841 aagccatatt tttctggtcg ccgatcgatt cttcttctgt caaaacggca tgactttttt
    39901 cagatgcttc tgattctccc gtcaaaatcg attcttccac taataaatca tgtgcttcga
    39961 ttaatcggat atctgctgga acgacatctc cggcacgtaa gacaatgaca tctcctttta
    40021 ccagttcttt cgcagggatt ttttcttgca tcccgtttga tagtactatc gcttcttgtc
    40081 ccatcagctg cttcaaacca ttcaatgatt cttccgcttt tctttcttgc cagtaactaa
    40141 cgagtccatt gacgatcact acgaataaaa taatacttcc ttcaatatat tcactagaag
    40201 caaatttcaa aaaagaggca atgatcaaca cgatcatcag cagatcagta aaatgctttg
    40261 ctattttttt ccatcgtttt gtctgctgcc ttgcttttat ttcattatat ccttgatcag
    40321 tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca
    40381 atacatcttc taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac
    40441 tgttattata cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat
    40501 aattcgtttt gcaaaatgta tctgggccta actgatattt ctattgctta ttcttttata
    40561 tacaaaattt aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct
    40621 ttcgaataac aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata
    40681 tctttgtgta gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga
    40741 agaccacaca aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg
    40801 actatcagct acaaaaaaat gtagacaaag accggcaaaa aataacattc ctgtaattaa
    40861 aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt
    40921 ttcttttgct gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga
    40981 caaagaagca accaaaatca ttagattgac aattgctgct ccgccttggc aatggatcac
    41041 ttgcagcata gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa
    41101 taaaatcgac aaaatcgttc ctatatagaa aaagagaata cgccaaggca gacagttgat
    41161 tgcttttttc aaattctttt gcggatcact tgtctcgcca acggttaagc cgatcatttc
    41221 aattccagca aaactgaata aaaccatagg aaatgagact accattccag caaagccaaa
    41281 cggtagtaat tcatcgatct gtccaaaatt ttctttgatt ccgatagact gggtatgaaa
    41341 atctgagaca ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat
    41401 gattgagaaa aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat
    41461 taagatcatt agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg
    41521 gatatacaat cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata
    41581 agtccaattg acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat
    41641 agagttaaat gtactatttg ctaaaaataa atcacccatg gcccgcatca aaccataaat
    41701 agccaaaccg catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt
    41761 tgctgaacct aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt
    41821 tcgattagtt agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt
    41881 caagcagcat ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg
    41941 gtactacaaa caaaatgaca ctgatggctg tgactgcaat tagattgatc catgttccag
    42001 ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc
    42061 aaactccaag attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg
    42121 catgtgtata acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga
    42181 taacagtagt tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat
    42241 aaaaaagtgc aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt
    42301 caaattgatt cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt
    42361 ctgtcactgt ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga
    42421 agacaagtaa actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa
    42481 tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta
    42541 caactaaagc attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc
    42601 gaatatacgt tttaactgga ttttccaatc gagtgatata cacagaggac atttcgatac
    42661 cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg
    42721 gtattaattt gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga
    42781 tccacgcagc aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa
    42841 caccgacttt tccgccgatt tttgccatat caaatttcaa attcaaaaga gtgatgatcc
    42901 aataaacgac taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat
    42961 cgataatatt accaaacaac ggagcgagtg ctgaagcgac catcaccatc ccaggaaaca
    43021 tttggaccca taacagccat gagacaacga acccccattt ttttcctaga gcattcgata
    43081 cccaaagttc tggaccacct ttttggggaa acattccagc aaattcacca gagatcaatg
    43141 agattggaaa agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg
    43201 ttgctgctac atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa
    43261 aggtcatggt cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca
    43321 atagcgacat atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt
    43381 catcgattca gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact
    43441 gggtttgata catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct
    43501 tggcgaggaa tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg
    43561 tctgttctcc atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt
    43621 tcgtttagtt tcttttccat tgctttggcg attttttttg tataaaaatt gaaaattcga
    43681 tcatcattca aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt
    43741 ttccattctt ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa
    43801 tcttcttgtg taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc
    43861 atataagaag accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta
    43921 cagccttgtt ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag
    43981 acttcgattg tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt
    44041 tgagctgcct cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat
    44101 acacgatgtg ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata
    44161 taagctccta acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa
    44221 gaaatgatat ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca
    44281 tagcccatct tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct
    44341 tcatatacct ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat
    44401 tcagctaagg aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca
    44461 tagccgccat atgcggcatc aacatgtaaa tagaaataga tcccttcatc ttttaacctt
    44521 tctcttaatt ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg
    44581 acagcaacca cacctagaat tgggattttt tgtccagcta attcacgaat tgttttttcc
    44641 aaagcattaa tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag
    44701 ccgactccgc aaatatctaa cgccttcatc caagaatagt gttttgtttg agggaccagc
    44761 catttcccta atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc
    44821 atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa
    44881 agttcccact cagacatttt tttcactttt tcaggcagca cttccttgac agctaaagga
    44941 atcgatttga tgcaacgggc gtaccataat ccttcaagat tagcaataga accatctgct
    45001 gcaatgtgtc cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct
    45061 gcttccattt gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc
    45121 atcgcatagt tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag
    45181 taacggcctg ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc
    45241 aagacctcta tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg
    45301 ctaattgcag gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc
    45361 atcttgttca acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg
    45421 gccttgatat ccatatcttt catttttctt ccctccatat tttgttggtc aatgtcttgc
    45481 agatacatgt gtttatctgt aacacctctt cactatagcg ccaaaaatga gaaatttttt
    45541 tttataacat caaatccacg tagattttta caaacaaaaa taaataaagt cggatttttc
    45601 tgataattca acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct aaaatagata
    45661 acctaaaaaa agcgaacaaa caaataaaat gactgtcctt cattcactaa acatattttt
    45721 atttttttat attgttaaaa attcacaatt tattttttaa tagaattatc tgttttccaa
    45781 attttataac ataaaaaacc ttttttaacg gcatttatat gctttttcag aaattaactg
    45841 atgatttcaa aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa aatacaaggc
    45901 ggaggctcta ctatgattga agaatatatt gagaaaaata ttttaagaca gctgttttta
    45961 tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat
    46021 gtttgcaaaa ccactttact caatgatatc aataacataa aaaaagaatt tgaagaacag
    46081 atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct
    46141 cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt
    46201 tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc
    46261 agcaaagctt attcattaaa aaaacaagta ttagcttact ttaatgactg cgggatcgaa
    46321 atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg
    46381 tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc
    46441 gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa
    46501 gaaatcttat cgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca
    46561 atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca
    46621 gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg
    46681 acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt
    46741 caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc
    46801 tttgaatctc acttcaatca agaattattc ggaaaccaac cgttcgaacg tgcgctgatt
    46861 catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa attttatctt
    46921 ttaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc
    46981 agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa
    47041 ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg
    47101 gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa
    47161 gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca
    47221 aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt
    47281 attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa
    47341 ggggctgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa
    47401 caatctcttg atacacgtca ggctcacaac ctcacttatt ttggacatct aggggtccga
    47461 tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga
    47521 aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt
    47581 ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc tcgcttactc
    47641 ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac
    47701 aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcga
    47761 aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt
    47821 ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat
    47881 aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc
    47941 aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc
    48001 attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa
    48061 tagaacaaga acgatcccga caattaagat accgatagaa caaatattta aaatagcaat
    48121 gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt
    48181 aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg
    48241 ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccatttt tctccctcca
    48301 tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc
    48361 atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata
    48421 gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa
    48481 tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat
    48541 tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg
    48601 agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat
    48661 ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca
    48721 atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca
    48781 caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg
    48841 tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg
    48901 atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa
    48961 accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc
    49021 gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga
    49081 tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac
    49141 gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag
    49201 accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca
    49261 atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt
    49321 ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct
    49381 atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa
    49441 cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag
    49501 gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc
    49561 aagatgaaat cactgtgact ggtttgaaaa ttaccaatac actgactcaa gaagaagaat
    49621 acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag
    49681 ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta
    49741 gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg
    49801 ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt
    49861 tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt
    49921 caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatt gtcgtgcatc
    49981 atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt
    50041 atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa
    50101 tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg
    50161 aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg
    50221 cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt
    50281 cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg
    50341 aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt attcctatgt
    50401 cccgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa
    50461 aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    50521 caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc
    50581 aagcttttga acaccgttta ccaagaggtt atgaaaaagc tgccctgcat caagtaataa
    50641 gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttagactta ttaccgcaga
    50701 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa aggcgtttag tttcgagtat
    50761 gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga
    50821 gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa
    50881 ctattttttc aacgtttact ctcttgccaa tccaacatca tctaacggat acaactattt
    50941 caatgattac cattacacta aaaaatcgga aacacacctc tccgaacaca aatacataag
    51001 actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct
    51061 ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc
    51121 atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga
    51181 tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg
    51241 cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt
    51301 aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa
    51361 agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa
    51421 tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg
    51481 atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta
    51541 tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt
    51601 tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg
    51661 cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt
    51721 tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc
    51781 ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga
    51841 attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg
    51901 attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc
    51961 tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt
    52021 ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt
    52081 caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgcaa tgattgcaga
    52141 aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca
    52201 agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg
    52261 aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga
    52321 gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gcttctattt
    52381 ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga ttttctatat
    52441 ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct atttttattt
    52501 ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga acatttttat
    52561 ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat gagaattttt
    52621 caaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg agcggtcgtc
    52681 ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata aaacgtggta
    52741 tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt tctgatttcc
    52801 gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa cagggcacaa
    52861 agatttggtg tcagatcagg gcggttatgg aagtcgtttt caatgacgcc cgctcctccg
    52921 acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc cttattagcg
    52981 attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt tgatgaaaac
    53041 caattcgcgc tttgttttac taattctggg tgttctctta atttgatcca gttatatgta
    53101 accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca agtctttatg
    53161 aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa cggaaaacaa tcacttgttc
    53221 tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta
    53281 ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac
    53341 cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag
    53401 gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta
    53461 agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc
    53521 caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa
    53581 ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg
    53641 cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg
    53701 cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt
    53761 gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga
    53821 taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga
    53881 ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa
    53941 ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa
    54001 ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta
    54061 caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca
    54121 tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac
    54181 cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa
    54241 gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat tcaccaccaa
    54301 aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc attactgcta
    54361 atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc gcaactaaat
    54421 gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata aatgcaacaa
    54481 atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa tcgacgatac
    54541 agattaatga tctcttttgc taaatctgta aaagctatag atgtcatcag atgatcttga
    54601 ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc
    54661 tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat tttttcatct
    54721 gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc gctttttgca
    54781 tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc ttccatttat
    54841 tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt cgagtacttt
    54901 ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa tgcctttagg
    54961 tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta aaagtaaaac
    55021 gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag caaagatgtc
    55081 tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga gacttgtact
    55141 cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca cgttcctttc
    55201 tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt
    55261 gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag aaagcgcaca
    55321 ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa aaaaagttgt
    55381 gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg
    55441 taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta ttcttattac
    55501 ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca gctgaccctg
    55561 ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc agtaaataca
    55621 atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa tccaattctc
    55681 tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg cttcgttcat
    55741 cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt ggtgacacac
    55801 agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct gttgctttga
    55861 tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc acgattcctt
    55921 tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt atcagccaag
    55981 ggatattatg ataattttct tttagataca tggctatatc atacatgatt ttcggataga
    56041 tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact tcataatatc
    56101 gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga acgcgtttag
    56161 gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct
    56221 ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac tcttctgtgt
    56281 agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca tacatataag
    56341 cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga tttaaaatac
    56401 agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta gccgttgcta
    56461 aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc ggccagcgaa
    56521 cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta aaccagtttt
    56581 ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa aaatcaacta
    56641 cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact tcataatgtt
    56701 ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt tcacttatat
    56761 gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa cgagcccaat
    56821 tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca tcctgctggt
    56881 attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa acaaaatgtt
    56941 catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct ttccaacctt
    57001 ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata gtaattttca
    57061 tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac ttttacaaat
    57121 ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac tgcaccgata
    57181 tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg gaccattaca
    57241 actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc agtgataaga
    57301 ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat accaaaaata
    57361 actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat ttgtttcata
    57421 tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac tccagacccg
    57481 cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac ttcttttcca
    57541 gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc
    57601 ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat aaacatcaat
    57661 agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa
    57721 atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc agccgcgata
    57781 cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac attcgttggc
    57841 acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca ataaagttca
    57901 atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt atattgttta
    57961 gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata aacagttgga
    58021 ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc cactttattc
    58081 agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa acttagtgtg
    58141 acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa gaaacttatc
    58201 tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc
    58261 gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat ataccttaaa
    58321 ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat aaaactcatt
    58381 ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc tattagtcag
    58441 cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat ataaaacaaa
    58501 aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta gtttcttttt
    58561 ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc attgcatcaa
    58621 acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga atatcgatcg
    58681 tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt gattcattaa
    58741 tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa
    58801 aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt
    58861 gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct gccaaacctg
    58921 ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc cgatcctgat
    58981 cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca ctgacaaatg
    59041 tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc acagcttctc
    59101 gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc ggcagtttat
    59161 ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta gttacttcta
    59221 ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta ttttttcgta
    59281 attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg attaatccgt
    59341 tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata aaattcactc
    59401 ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat aacagacgat
    59461 cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa aaagtttgat
    59521 tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt gagaaatcta
    59581 aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct ccgaccagta
    59641 cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct aatgattgtc
    59701 ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat ccaacttgtt
    59761 cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga ataccatgta
    59821 gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc cctagccctt
    59881 cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac atattgtagc
    59941 gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc attttttttg
    60001 tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt cttacaagtt
    60061 gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga tgatgataga
    60121 agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt gaaatgatgg
    60181 atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct gctttgctag
    60241 aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc ccaaagtttt
    60301 tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg gtaattccca
    60361 tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa tcaccaaaat
    60421 tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg gatttacgat
    60481 attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct cctcatcaaa
    60541 tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa gaaaaggagt
    60601 taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg ttgatagatc
    60661 agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt tttgtaaaat
    60721 tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt tgtgacgatt
    60781 ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc taattctttt
    60841 tgtcgttcga cttgataaaa aatcactgga cgatattgct tccctctatc ttgaaattgt
    60901 ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc gctataactt
    60961 atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta accaccactt
    61021 acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa aacagatagg
    61081 atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc tgcaaatact
    61141 gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata tttttgctcc
    61201 ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt cagattcatt
    61261 cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt aacggatata
    61321 ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc tcgtgtattc
    61381 ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc catagattct
    61441 cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata aagtgaggaa
    61501 ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga atccttatcg
    61561 acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta ccttttatgg
    61621 aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata agaaataata
    61681 gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac tccgcttatt
    61741 tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat ttctacattc
    61801 gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt agttactcta
    61861 tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact gccaatcttt
    61921 gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga ataatcatat
    61981 aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt tacaacaata
    62041 tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac agttaaagtg
    62101 attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt gtatcttcta
    62161 tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa gcttaagatc
    62221 cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca tggcaccaaa
    62281 ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc tatgtttgtt
    62341 aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag tcccattgag
    62401 taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt agatgcatga
    62461 gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg ttcaaacatc
    62521 ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa taatttttta
    62581 aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat aaaaataaaa
    62641 atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac tagcaaactc
    62701 atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat gatttgataa
    62761 gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat tgcggaacga
    62821 tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa taaaaatgcc
    62881 gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt tgtacacaag
    62941 ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat ccaatataac
    63001 attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata tccagtataa
    63061 ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt gattcgagat
    63121 aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa aaagatgaaa
    63181 gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt atttattttc
    63241 attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact tattaggtgc
    63301 aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga tccataaagc
    63361 catgagagca gttggagtat attttgttgt aatttctatc ttattttctc cagcatttac
    63421 atccacattc actaagccat gttttttata agagtcattt acgactttac cgtttacttt
    63481 tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt tgttgcttgc
    63541 cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc acgattctcc
    63601 tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg ttggggcgta
    63661 atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt cattgataac
    63721 aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg ctatagacgt
    63781 gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt tattcatagt
    63841 ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga tacctaagat
    63901 acgccaagga aactggagta agcttatcgg tgttttttga aacaaaaacc atggaaataa
    63961 attagttacg catacccatg tagaaactac gccaacatag atgatttttt cttttttctc
    64021 catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata accccatagt
    64081 gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag aaagtgcttc
    64141 tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct gctgcaacat
    64201 aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta ctaaattcac
    64261 gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa ctataatcat
    64321 acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca caagtagact
    64381 tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc cataagcata
    64441 atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg atcgataata
    64501 gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa aagcagcatc
    64561 tatcttttta aaaaactttt ctccataata aaaactaatc agaatcgtac aaatatttaa
    64621 taaccaaata tatagaacat aacttaatat taagttattt gaaattccat aaaaaacgac
    64681 tgcaggaatt attgtaagaa aaggataaaa atagttcagt ccatctccat aattgttaaa
    64741 tgttgtatag ttgattggcc cttcaaaaat agaagataac cctttgattc gtgaaagatg
    64801 aaaagataaa tcacctgttg tataaatttc ttgagcattt ctcataaaat ttactgaata
    64861 taaagtacta agtaagataa gaactactaa aagaaatgct tttttactta ttttcataga
    64921 tttgttgttc tcctttttta attctatttt cagtatacta gaattgtctt tttttaataa
    64981 gttttgcgtt aaatttttca tagcactagt atgatatact agaaaagaat caatttttat
    65041 tacttttaaa taggagaaaa aaatgaaaaa aaacaaaaac caaataatag a
//
LOCUS       DACDSR010000006        61299 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDSR010000006 DACDSR010000000
VERSION     DACDSR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.006"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..561)
                     /locus_tag="IWK15_000409"
     CDS             complement(<1..561)
                     /locus_tag="IWK15_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9546040.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="IWK15_000410"
     CDS             complement(748..1398)
                     /locus_tag="IWK15_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546041.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IWK15_000411"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IWK15_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9546042.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="IWK15_000412"
     CDS             complement(2910..3908)
                     /locus_tag="IWK15_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9546043.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IWK15_000413"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IWK15_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9546044.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="IWK15_000414"
     CDS             complement(4924..5655)
                     /locus_tag="IWK15_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9546045.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="IWK15_000415"
     CDS             complement(5659..6924)
                     /locus_tag="IWK15_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9546046.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IWK15_000416"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IWK15_000416"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9546047.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IWK15_000417"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IWK15_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9546048.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="IWK15_000418"
     CDS             12266..13768
                     /locus_tag="IWK15_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9546049.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="IWK15_000419"
     CDS             13770..14507
                     /locus_tag="IWK15_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9546050.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="IWK15_000420"
     CDS             complement(14552..14725)
                     /locus_tag="IWK15_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9546051.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="IWK15_000421"
     CDS             14701..15366
                     /locus_tag="IWK15_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9546052.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="IWK15_000422"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="IWK15_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9546053.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="IWK15_000423"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="IWK15_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9546054.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="IWK15_000424"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="IWK15_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9546055.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="IWK15_000425"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="IWK15_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9546056.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="IWK15_000426"
     CDS             complement(20579..21745)
                     /locus_tag="IWK15_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9546057.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="IWK15_000427"
     CDS             complement(21761..21988)
                     /locus_tag="IWK15_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9546058.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="IWK15_000428"
     CDS             22472..23062
                     /locus_tag="IWK15_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9546059.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="IWK15_000429"
     CDS             23252..23965
                     /locus_tag="IWK15_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546060.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="IWK15_000430"
     CDS             complement(24080..25090)
                     /locus_tag="IWK15_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9546061.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="IWK15_000431"
     CDS             complement(25127..25609)
                     /locus_tag="IWK15_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9546062.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="IWK15_000432"
     CDS             25759..26127
                     /locus_tag="IWK15_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9546063.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="IWK15_000433"
     CDS             26592..27044
                     /locus_tag="IWK15_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9546064.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="IWK15_000434"
     CDS             complement(27168..28019)
                     /locus_tag="IWK15_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9546065.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="IWK15_000435"
     CDS             complement(28032..28817)
                     /locus_tag="IWK15_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9546066.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="IWK15_000436"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="IWK15_000436"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9546067.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="IWK15_000437"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="IWK15_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9546068.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="IWK15_000438"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="IWK15_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="IWK15_000439"
     CDS             34215..34388
                     /locus_tag="IWK15_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546069.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="IWK15_000440"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="IWK15_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9546070.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="IWK15_000441"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="IWK15_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9546071.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="IWK15_000442"
     CDS             complement(37238..37549)
                     /locus_tag="IWK15_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9546072.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="IWK15_000443"
     CDS             complement(37546..37839)
                     /locus_tag="IWK15_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9546073.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="IWK15_000444"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="IWK15_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9546074.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="IWK15_000445"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="IWK15_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9546075.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="IWK15_000446"
     CDS             complement(39677..44035)
                     /locus_tag="IWK15_000446"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9546076.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="IWK15_000447"
     CDS             complement(44247..45956)
                     /locus_tag="IWK15_000447"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9546077.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="IWK15_000448"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="IWK15_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9546078.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="IWK15_000449"
     CDS             complement(47453..48253)
                     /locus_tag="IWK15_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9546079.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="IWK15_000450"
     CDS             complement(48250..49062)
                     /locus_tag="IWK15_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9546080.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="IWK15_000451"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="IWK15_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9546081.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="IWK15_000452"
     CDS             complement(49817..50539)
                     /locus_tag="IWK15_000452"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9546082.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="IWK15_000453"
     CDS             complement(50675..51556)
                     /locus_tag="IWK15_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9546083.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="IWK15_000454"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="IWK15_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9546084.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="IWK15_000455"
     CDS             52796..53275
                     /locus_tag="IWK15_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9546085.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="IWK15_000456"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="IWK15_000456"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9546086.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="IWK15_000457"
     CDS             54576..54704
                     /locus_tag="IWK15_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9546087.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="IWK15_000458"
     CDS             54708..55253
                     /locus_tag="IWK15_000458"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9546088.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="IWK15_000459"
     CDS             complement(55707..57398)
                     /locus_tag="IWK15_000459"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9546089.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="IWK15_000460"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="IWK15_000460"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9546090.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="IWK15_000461"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="IWK15_000461"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9546091.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="IWK15_000462"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="IWK15_000462"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9546092.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17033 a        13093 c        10618 g        20555 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattca
//
LOCUS       DACDSR010000007        60192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDSR010000007 DACDSR010000000
VERSION     DACDSR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.007"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK15_000463"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK15_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9546093.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK15_000464"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK15_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9546094.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK15_000465"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK15_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9546095.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWK15_000466"
     CDS             1642..2274
                     /locus_tag="IWK15_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9546096.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWK15_000467"
     CDS             complement(2276..3181)
                     /locus_tag="IWK15_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9546097.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWK15_000468"
     CDS             complement(3266..3919)
                     /locus_tag="IWK15_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9546098.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWK15_000469"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWK15_000469"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9546099.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK15_000470"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK15_000470"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9546100.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWK15_000471"
     CDS             6168..7253
                     /locus_tag="IWK15_000471"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9546101.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWK15_000472"
     CDS             7250..8314
                     /locus_tag="IWK15_000472"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9546102.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWK15_000473"
     CDS             complement(8563..9366)
                     /locus_tag="IWK15_000473"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9546103.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK15_000474"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK15_000474"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9546104.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWK15_000475"
     CDS             complement(10244..11251)
                     /locus_tag="IWK15_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9546105.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWK15_000476"
     CDS             complement(11258..11902)
                     /locus_tag="IWK15_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9546106.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWK15_000477"
     CDS             complement(12002..12760)
                     /locus_tag="IWK15_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9546107.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK15_000478"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK15_000478"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9546108.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK15_000479"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK15_000479"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9546109.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK15_000480"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK15_000480"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9546110.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK15_000481"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK15_000481"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9546111.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK15_000482"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK15_000482"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9546112.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWK15_000483"
     CDS             18158..19027
                     /locus_tag="IWK15_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9546113.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWK15_000484"
     CDS             complement(19153..20052)
                     /locus_tag="IWK15_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9546114.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWK15_000485"
     CDS             complement(20217..21041)
                     /locus_tag="IWK15_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9546115.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWK15_000486"
     CDS             complement(21052..22347)
                     /locus_tag="IWK15_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9546116.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWK15_000487"
     CDS             complement(22363..22842)
                     /locus_tag="IWK15_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9546117.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWK15_000488"
     CDS             complement(22848..23876)
                     /locus_tag="IWK15_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9546118.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWK15_000489"
     CDS             complement(23893..24999)
                     /locus_tag="IWK15_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9546119.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK15_000490"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK15_000490"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9546120.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWK15_000491"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWK15_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9546121.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWK15_000492"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWK15_000492"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9546122.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IWK15_000493"
     CDS             29970..30668
                     /locus_tag="IWK15_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9546123.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IWK15_000494"
     CDS             complement(30836..33475)
                     /locus_tag="IWK15_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9546124.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWK15_000495"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="IWK15_000495"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9546125.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="IWK15_000496"
     CDS             35318..36493
                     /locus_tag="IWK15_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9546126.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="IWK15_000497"
     CDS             36497..37138
                     /locus_tag="IWK15_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546127.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="IWK15_000498"
     CDS             37135..38052
                     /locus_tag="IWK15_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9546128.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="IWK15_000499"
     CDS             38049..38720
                     /locus_tag="IWK15_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546129.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="IWK15_000500"
     CDS             complement(39261..39749)
                     /locus_tag="IWK15_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9546130.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="IWK15_000501"
     CDS             complement(39950..40216)
                     /locus_tag="IWK15_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9546131.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="IWK15_000502"
     CDS             complement(40216..40479)
                     /locus_tag="IWK15_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9546132.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(40472..40744)
                     /locus_tag="IWK15_000503"
     CDS             complement(40472..40744)
                     /locus_tag="IWK15_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546133.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            40955..41140
                     /locus_tag="IWK15_000504"
     CDS             40955..41140
                     /locus_tag="IWK15_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9546134.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="IWK15_000505"
     CDS             complement(41282..43105)
                     /locus_tag="IWK15_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9546135.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="IWK15_000506"
     CDS             complement(43703..46408)
                     /locus_tag="IWK15_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9546136.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="IWK15_000507"
     CDS             complement(46714..47661)
                     /locus_tag="IWK15_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9546137.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="IWK15_000508"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="IWK15_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9546138.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="IWK15_000509"
     CDS             complement(48296..48658)
                     /locus_tag="IWK15_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9546139.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="IWK15_000510"
     CDS             complement(48669..49790)
                     /locus_tag="IWK15_000510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9546140.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="IWK15_000511"
     CDS             complement(49806..50156)
                     /locus_tag="IWK15_000511"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9546141.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="IWK15_000512"
     CDS             complement(50286..51797)
                     /locus_tag="IWK15_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9546142.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="IWK15_000513"
     CDS             complement(52119..53471)
                     /locus_tag="IWK15_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9546143.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="IWK15_000514"
     CDS             complement(53624..54700)
                     /locus_tag="IWK15_000514"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9546144.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="IWK15_000515"
     CDS             54896..56641
                     /locus_tag="IWK15_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9546145.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="IWK15_000516"
     CDS             complement(56921..57358)
                     /locus_tag="IWK15_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9546146.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="IWK15_000517"
     CDS             complement(57342..58715)
                     /locus_tag="IWK15_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546147.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="IWK15_000518"
     CDS             complement(59037..59525)
                     /locus_tag="IWK15_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546148.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="IWK15_000519"
     CDS             complement(59547..60149)
                     /locus_tag="IWK15_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9546149.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19083 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ct
//
LOCUS       DACDSR010000008        51993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDSR010000008 DACDSR010000000
VERSION     DACDSR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.008"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            234..3068
                     /locus_tag="IWK15_000520"
     CDS             234..3068
                     /locus_tag="IWK15_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9546150.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3259..3750
                     /locus_tag="IWK15_000521"
     CDS             3259..3750
                     /locus_tag="IWK15_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9546151.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3853..4842
                     /gene="manX"
                     /locus_tag="IWK15_000522"
     CDS             3853..4842
                     /gene="manX"
                     /locus_tag="IWK15_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9546152.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4875..5678
                     /locus_tag="IWK15_000523"
     CDS             4875..5678
                     /locus_tag="IWK15_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9546153.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5699..6613
                     /locus_tag="IWK15_000524"
     CDS             5699..6613
                     /locus_tag="IWK15_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9546154.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6696..7070
                     /locus_tag="IWK15_000525"
     CDS             6696..7070
                     /locus_tag="IWK15_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9546155.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7209..7454
                     /locus_tag="IWK15_000526"
     CDS             7209..7454
                     /locus_tag="IWK15_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9546156.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7626..8690
                     /locus_tag="IWK15_000527"
     CDS             7626..8690
                     /locus_tag="IWK15_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9546157.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            8936..9361
                     /locus_tag="IWK15_000528"
     CDS             8936..9361
                     /locus_tag="IWK15_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9546158.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9354..9803
                     /gene="nusB"
                     /locus_tag="IWK15_000529"
     CDS             9354..9803
                     /gene="nusB"
                     /locus_tag="IWK15_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9546159.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9900..10754
                     /locus_tag="IWK15_000530"
     CDS             9900..10754
                     /locus_tag="IWK15_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9546160.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10755..12092
                     /locus_tag="IWK15_000531"
     CDS             10755..12092
                     /locus_tag="IWK15_000531"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9546161.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12097..12321
                     /locus_tag="IWK15_000532"
     CDS             12097..12321
                     /locus_tag="IWK15_000532"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9546162.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12323..13213
                     /locus_tag="IWK15_000533"
     CDS             12323..13213
                     /locus_tag="IWK15_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9546163.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13222..14040
                     /locus_tag="IWK15_000534"
     CDS             13222..14040
                     /locus_tag="IWK15_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9546164.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14147..14596
                     /locus_tag="IWK15_000535"
     CDS             14147..14596
                     /locus_tag="IWK15_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9546165.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14614..16296
                     /gene="recN"
                     /locus_tag="IWK15_000536"
     CDS             14614..16296
                     /gene="recN"
                     /locus_tag="IWK15_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9546166.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16351..16467)
                     /locus_tag="IWK15_000537"
     CDS             complement(16351..16467)
                     /locus_tag="IWK15_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9546167.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16540..17496)
                     /locus_tag="IWK15_000538"
     CDS             complement(16540..17496)
                     /locus_tag="IWK15_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9546168.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17667..18035
                     /locus_tag="IWK15_000539"
     CDS             17667..18035
                     /locus_tag="IWK15_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9546169.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18205..18636
                     /gene="mraZ"
                     /locus_tag="IWK15_000540"
     CDS             18205..18636
                     /gene="mraZ"
                     /locus_tag="IWK15_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9546170.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18657..19616
                     /gene="rsmH"
                     /locus_tag="IWK15_000541"
     CDS             18657..19616
                     /gene="rsmH"
                     /locus_tag="IWK15_000541"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9546171.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19621..20022
                     /gene="ftsL"
                     /locus_tag="IWK15_000542"
     CDS             19621..20022
                     /gene="ftsL"
                     /locus_tag="IWK15_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9546172.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20023..22215
                     /locus_tag="IWK15_000543"
     CDS             20023..22215
                     /locus_tag="IWK15_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9546173.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22241..23203
                     /locus_tag="IWK15_000544"
     CDS             22241..23203
                     /locus_tag="IWK15_000544"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9546174.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23217..24578
                     /locus_tag="IWK15_000545"
     CDS             23217..24578
                     /locus_tag="IWK15_000545"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9546175.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24604..25692
                     /gene="murG"
                     /locus_tag="IWK15_000546"
     CDS             24604..25692
                     /gene="murG"
                     /locus_tag="IWK15_000546"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9546176.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25707..26927
                     /locus_tag="IWK15_000547"
     CDS             25707..26927
                     /locus_tag="IWK15_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9546177.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27083..28408
                     /gene="ftsA"
                     /locus_tag="IWK15_000548"
     CDS             27083..28408
                     /gene="ftsA"
                     /locus_tag="IWK15_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9546178.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28431..29672
                     /gene="ftsZ"
                     /locus_tag="IWK15_000549"
     CDS             28431..29672
                     /gene="ftsZ"
                     /locus_tag="IWK15_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9546179.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29689..30366
                     /locus_tag="IWK15_000550"
     CDS             29689..30366
                     /locus_tag="IWK15_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9546180.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30380..30979
                     /locus_tag="IWK15_000551"
     CDS             30380..30979
                     /locus_tag="IWK15_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9546181.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            30994..31281
                     /locus_tag="IWK15_000552"
     CDS             30994..31281
                     /locus_tag="IWK15_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9546182.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31330..32112
                     /locus_tag="IWK15_000553"
     CDS             31330..32112
                     /locus_tag="IWK15_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9546183.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32133..32840
                     /locus_tag="IWK15_000554"
     CDS             32133..32840
                     /locus_tag="IWK15_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9546184.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33102..35900
                     /gene="ileS"
                     /locus_tag="IWK15_000555"
     CDS             33102..35900
                     /gene="ileS"
                     /locus_tag="IWK15_000555"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9546185.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(35949..37475)
                     /locus_tag="IWK15_000556"
     CDS             complement(35949..37475)
                     /locus_tag="IWK15_000556"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9546186.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37490..38137)
                     /locus_tag="IWK15_000557"
     CDS             complement(37490..38137)
                     /locus_tag="IWK15_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9546187.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38321..38650)
                     /locus_tag="IWK15_000558"
     CDS             complement(38321..38650)
                     /locus_tag="IWK15_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9546188.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38827..39555
                     /gene="sfsA"
                     /locus_tag="IWK15_000559"
     CDS             38827..39555
                     /gene="sfsA"
                     /locus_tag="IWK15_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9546189.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39571..40584
                     /locus_tag="IWK15_000560"
     CDS             39571..40584
                     /locus_tag="IWK15_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9546190.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40584..41861
                     /locus_tag="IWK15_000561"
     CDS             40584..41861
                     /locus_tag="IWK15_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9546191.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            41924..44626
                     /locus_tag="IWK15_000562"
     CDS             41924..44626
                     /locus_tag="IWK15_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9546192.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44778..45095
                     /locus_tag="IWK15_000563"
     CDS             44778..45095
                     /locus_tag="IWK15_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9546193.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45125..45445
                     /locus_tag="IWK15_000564"
     CDS             45125..45445
                     /locus_tag="IWK15_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9546194.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45553..47013
                     /locus_tag="IWK15_000565"
     CDS             45553..47013
                     /locus_tag="IWK15_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546195.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47081..47302
                     /locus_tag="IWK15_000566"
     CDS             47081..47302
                     /locus_tag="IWK15_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546196.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47333..47515
                     /locus_tag="IWK15_000567"
     CDS             47333..47515
                     /locus_tag="IWK15_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9546197.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47515..47928
                     /locus_tag="IWK15_000568"
     CDS             47515..47928
                     /locus_tag="IWK15_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9546198.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48051..49232
                     /locus_tag="IWK15_000569"
     CDS             48051..49232
                     /locus_tag="IWK15_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546199.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49621..49694
                     /locus_tag="IWK15_000570"
     tRNA            49621..49694
                     /locus_tag="IWK15_000570"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49655..49657,aa:Arg,seq:cct)
     gene            complement(49763..50902)
                     /locus_tag="IWK15_000571"
     CDS             complement(49763..50902)
                     /locus_tag="IWK15_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9546200.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(51016..51600)
                     /locus_tag="IWK15_000572"
     CDS             complement(51016..51600)
                     /locus_tag="IWK15_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9546201.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(51727..>51993)
                     /locus_tag="IWK15_000573"
     CDS             complement(51727..>51993)
                     /locus_tag="IWK15_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP9546202.1"
                     /translation="HFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENEL
                     TDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT        17204 a         8804 c        11085 g        14900 t
ORIGIN      
        1 agctatattg tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt
       61 tccattataa aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc
      121 cgcgtgttat caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata
      181 gtattatgtt ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa
      241 aacgcattga ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag
      301 aatatgatca aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa
      361 atagcagtaa agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac
      421 gaccagtacg ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg
      481 tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta
      541 aggatatctt tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg
      601 caaaagctgc gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg
      661 gatcaggaaa gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa
      721 tagatgagga aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc
      781 ttttgatgag tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa
      841 aaatggggat tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt
      901 tgccgcctga aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc
      961 ttggggaaac aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag
     1021 acccaggttc ttctctatta gaaacttttg ttcggcgtat cccaatcaat atcaaacttc
     1081 cggcttttga aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc
     1141 acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga
     1201 tcggcagtgt gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg
     1261 cacgaggatt tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga
     1321 cagaagggat tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc
     1381 tttctaaaat attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc
     1441 aatcggactc ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt
     1501 tactgaagtc ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca
     1561 acatccatct gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat
     1621 tagcagaatt tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca
     1681 aaagatcttt accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca
     1741 gttctttttt gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg
     1801 agatggcttt tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg
     1861 aaacgtattt tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg
     1921 tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa
     1981 acagcacagc aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac
     2041 gagcagtaga catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca
     2101 atgtacaaga agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg
     2161 cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg
     2221 taacaacttc tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag
     2281 atatgttgca tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat
     2341 ctgttcagga agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg
     2401 gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag
     2461 aaacagaatt agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta
     2521 tccaaaaaga atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc
     2581 catttattcc tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc
     2641 tgatggaatc agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa
     2701 atacgtgtgt tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg
     2761 atccaatgtg gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat
     2821 atggattcaa aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga
     2881 atgaaccatt gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata
     2941 gccaattggc agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag
     3001 aagaaatgta ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg
     3061 tgccatgaat acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa
     3121 gaatccttta aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta
     3181 gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag
     3241 gaagaaggag ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac
     3301 gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc
     3361 gatgtagtcg ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg
     3421 atcacagcaa atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta
     3481 tcacgagcaa aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc
     3541 tgcggtgtac agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca
     3601 atgattacca attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac
     3661 agtcaagaag tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg
     3721 gaactactag cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa
     3781 ttctgttgta atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata
     3841 ggaggtataa atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc
     3901 ttacaatccg gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa
     3961 ccaagtgaag gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat
     4021 aatcaagacg aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca
     4081 aacacattat tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca
     4141 atgttgattg aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca
     4201 cacatcattg aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca
     4261 gaagcaccga aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact
     4321 gtggtcggag acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat
     4381 ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca
     4441 gatgcagtat caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt
     4501 gtaaaagcaa atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt
     4561 ggaaatacca aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa
     4621 ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc
     4681 gttgtaagta aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa
     4741 caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat
     4801 gatattttga aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt
     4861 ataggaggct tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag
     4921 ctggtatgga aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat
     4981 tgatcggttt agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga
     5041 tgatcgcttt aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat
     5101 cagtagcatc agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg
     5161 cgatcgcgat tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta
     5221 caatcgctgt tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta
     5281 aggttgaaat gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag
     5341 cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag
     5401 catggttaac agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt
     5461 tggtaatcaa catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg
     5521 tagctgcaat ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc
     5581 ttatttactt gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact
     5641 ctggtgaccc tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat
     5701 ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac
     5761 cttcattcaa ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat
     5821 gattccagca atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg
     5881 tcacttggaa ttctttaata cacacccata cattgcttct ccaattttag gggttacatt
     5941 ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt
     6001 taaagtaggt atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt
     6061 acgtccaatg ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc
     6121 aatcatcttc ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga
     6181 atttggctac aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga
     6241 cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg
     6301 ggtatcaatc aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat
     6361 cgaatgggat aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa
     6421 taatggctta gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt
     6481 gattcctggt ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa
     6541 ggtttctcca atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat
     6601 cggactttta taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag
     6661 tatactgttc ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag
     6721 tagacttggt aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga
     6781 taggtgataa tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc
     6841 catgggaaga agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc
     6901 gatatgccat tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa
     6961 aagtgcttcg tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt
     7021 tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga
     7081 aaacttctaa gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc
     7141 aaaaccaatt atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag
     7201 gttatattat gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag
     7261 gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag
     7321 tcggttcaag tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg
     7381 cacttattcc atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg
     7441 gaaaacgatc ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga
     7501 ctcctttttg gctttattta tttaaaataa aaagaagttt tccggaaatt tttatttaac
     7561 tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc gtgcaaagaa agagggagtt
     7621 tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat gcaagaagaa aatttggatt
     7681 catttttggt taccagtccg tacaatcttc gttacttaac gaactttaca ggtacgacag
     7741 gattagctgt tattactttg gaaaaagcat tttttattac tgattttcgt tataccgagc
     7801 aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa tgtcggacct atttttgatg
     7861 aggtagcaaa cttagtacgc aaagaagaac tgcactcatt agcttttgag gaaacaacag
     7921 tctcattttt agaatattcg gttttagaag aaatcatcga tgcggaatta gtacctgttt
     7981 cacagatgat cgaagaactc agagaagtga aagatgaaga agaaatcgct attatcgaaa
     8041 aagcatgtca tattgcggat atggcatatg atcacatcct gaaaatgatc caaccgggca
     8101 tgactgagat tgaagtcgca aatcaactag acttttatat gcgttctctt ggcgcttcag
     8161 gtgtttcttt tgatacgatc gttgctagcg gattgcgttc ggctatgccg catggtgtag
     8221 caagcaaaaa aatcatcgaa caaggtgact tgatcacatt ggactttggt tgctattacg
     8281 aagggtacgt ttccgatatg acacgtacat tcgctattgg tgatcctggg gaaaaattaa
     8341 aagaaattta tcaaatagtc ttagatgctc aattagcagt tattgatgct gcaaaaccag
     8401 gaatgactgg tgtccaatta gatgcagttg ctagagatta tatttcaaaa catggttacg
     8461 gggaagcatt tggacattct acaggacatg ggatcggttt agagatccac gaaggaccaa
     8521 acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa tgtgatcact gatgaaccag
     8581 ggatctactt accaggaatc ggtggagtac gtattgagga tgacttgttg atcacagaag
     8641 atggaaatcg tgtgttgact cgttcaccaa aagaattgat catcttatag tttcatcaat
     8701 agaagaagta agcgtaaaaa tcagtgttca cagactttgt tatagaaaat cactcgattt
     8761 aatgatacac tatagaagaa cttttaagct gtaagagata ataaagttgg gtgttttctc
     8821 caattcatag cagtttgcag ctgatcggcg gcagttttct taccacgtga tcactatgcc
     8881 gcatcataga gaattcggat gtatctcgtg cttaggcaaa gggaggaata aataaatggc
     8941 agatgaaaaa aatttgattt taaacgcaaa ccaagattta ggagagatcg tgatcgctcc
     9001 tgaagtcatc gaagtaatta tcgggattgc agcttctaaa gtagaaggcg tatacggaat
     9061 gcgcggaact tttgccagca atgtcactga attattagga cgtgcagctc acggaaaagg
     9121 tgtttactta gcaaacgagg aagatggctt gaaagtagat ctttactgtt atctagaata
     9181 tggtgtttca gttcctaaag tagcaatgga aatgcaggaa cgggtcagcc agcaagtcct
     9241 tttcatgacg gatatcgatt tatctgaagt caatatccat gtagtagctg ttgttcctga
     9301 aaaattagaa caaccaacaa tcgaagaact ttttgaagac gaagaggaag agaatgaata
     9361 aagaattatc aagacacgag attcgggaaa tggcattgca ggctttattt ccattagact
     9421 tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc aatcgaatta gaccaccgcg
     9481 acatgatcaa cgaagatgaa tcagagtttg ttcctgttta tcttgatacg ctagttggcg
     9541 gtgtctgtgc aaaaaaagat gaattggata aagtgattga aaaacattta aaaaacaatt
     9601 ggcacatcaa tcggatctca aaaatggatt tggtgatctt gcgtatcgcg atttttgaaa
     9661 tgacttatgt gacagatgta ccagcggctg ttgctttaaa tgaagcaatc gagttggcaa
     9721 aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg tgttttatcc aatgtattga
     9781 aagaattaga agctggggcg taagtcctag cttttttcta tgaatcatca taaacctgtt
     9841 ataaatcggg aaaattctat ggtagaatga aaacacaaaa aatatctggg agtggttaga
     9901 tggcagaatt gattaacgga aaagaattag ctgaaaaaat gcaggcagag attgctgaaa
     9961 aaatccaaaa attgaaagat aatggtatcc atcccggatt agtcgtttta ttggtagggg
    10021 aaaatccagc cagccaaatc tatgtaagaa ataaagaacg tgtagcaaaa gaaatcggaa
    10081 tccattcatt ggtagagcgt tatcctgaga cgatttctga agacgaacta cttgcagaaa
    10141 ttgaaaaata caatcaagat cctagttttc atggtatatt ggtccaatta ccattgccaa
    10201 agcatataga tgaagaaaag atcctgttag ctatccgccc tgaaaaagac gtagacggtt
    10261 ttcatccgat gaatttaggc agactccttg cgggtaaacc ggacaagatt ccttgtactc
    10321 catacggcat catgaaaatg tttgaagctt attctatcga tccggcgggt aaacgagcag
    10381 tagttatcgg acgaagcaat atcgtcggta aaccgatggc tcagctactc ttgatggcag
    10441 atgcaactgt tactgttgct cattctaaaa ccgaaaatct ggctgaactg gcacaagaag
    10501 cagatatttt agtcgtagca ataggacgtg gacattttgt cacaaaagaa ttcgtaaaac
    10561 caggtgctgt agtgatagat gtagggatga atcgtgatgc taacggtaag ctgatcggtg
    10621 atgtgaaatt tgatgaagtg gagcctattg caggctggat cactcctgtg ccaaagggtg
    10681 tagggccaat gactatcacg atgctgatgt atcaaaccgt aaaatctgcc gaagcttttg
    10741 gagcaggtga atagatggca caagaatatt taactgtcac tacattaacg aagtatttga
    10801 aaagaaaatt tgatgccgat ccttatttag aacgcgtata tttaacaggc gaaatatcga
    10861 atttccgttt acgtgccaat gcccatcaat atttcagctt aaaagatgac catgcaaaaa
    10921 tatctgctat tatgttcaaa ggagcattcc aaaaattacg attccagcca aaagaaggaa
    10981 tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc gggaggttct tatcaaatct
    11041 atgtagagca catggagcct gatggaatag gtgctttata tcaagaattg gaagaacgaa
    11101 aagaaaagct gatgaaggaa ggcctttttc aagggcctaa aaaaatgctg ccacgtttcc
    11161 caaagcgaat cgcggtcctt accagtccaa gcggagcggt tatccgcgat atcatcacga
    11221 cggtgaaaag aagatatcca attgtccaat tggttttgtt cccaacagtt gtacaaggaa
    11281 accaagcagc ggacaatgtt gtcaggaata tccagagagt agaagagatg ggcaatttcg
    11341 atacaatgat catcggtcgt gggggtggtt cgattgagga tttatggccg tttaatgaag
    11401 agcgggttgc tcgggcgatt tatcaagcta atacgccggt gatttcatct gtaggacatg
    11461 aaacagatac cacgatcgct gatttagtag cagatgttcg ggctgccact ccaacagcag
    11521 ctgcagaatt agctgttcct gttttgtcag aagagttgat gcgcattcaa gaacggcaag
    11581 ctagattgca gcaggcgttt gtacgccaga tccagcgcaa acaagaacgg ttcgagcgtg
    11641 ctcgacgttc ctatgtattt cggcagcctg aaagattgta tgaaggtcag accatcaaat
    11701 tagaccagtt gaaacagcga ttatatcaag ctacacagca aatttatcat gcgaaagaaa
    11761 aacaaacgat cgcattaagc caccaattgg aacaagtggc tccgctttat cgagttaaag
    11821 cagctcgcca agaaactgct tatctagaaa aacgcttaga agagaaaatc caacaatata
    11881 tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc tcttgatttg ttgagtccat
    11941 tgaaaatcat gggaagaggc tacagctata cgacgttaaa tgatcaagta gtaaaaagtg
    12001 tcaccgagat tcatcaaggg gatttgctga cgatccatta tcaagatgga atcgttaaag
    12061 gacaagtaca agagatagac aaaggagaat aaaaatatgg aaaatccaac ttttgaagag
    12121 tcactgcaag aattagaaaa aattgtcatg cagttagaac aaggagacgt tccattagaa
    12181 tcagctctag attcgtttaa acgaggaatg gaattaagca aacattgcca agatacatta
    12241 gctaaagcag aagagacctt gacgaaaatg atgacagaat caaatgaaga agttgatttt
    12301 gacggaaacg aggaaagctg agatgagatt aactgacttt tcagctattc atctacctgc
    12361 tgtagaaaaa gaaatcttgt cttttttaga tgagcataca acggatagag gactctatga
    12421 tgcgatgagt tattcagtga aagcaggcgg gaaaagaata cgtcccttat tacttttaac
    12481 ggccgttgct tcctttgatg agcctattga tgttcccgtc tatcaagtag cagctgcttt
    12541 agaaatggtg catacgtatt ccttgatcca cgatgatttg cctgcgatgg acaatgatga
    12601 tcttcgtaga ggaaaaccta ccaatcataa agtattcggc gaagcattag ctatattggc
    12661 cggagatggt ttgcttacag gagcattcca gctaatcagt atggcacact taggtaattc
    12721 tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg ggatcacaag gtatggttgc
    12781 tgggcaagct gcagatatcg agggcgaatc gaaaaagctt tctttagaag aattagcatt
    12841 tattcatgaa agaaagacag ggcatttgat tcgttatgcc ttgctggctg gtggcatatt
    12901 agcgaagcaa cctgaagaaa tcttattgct acttcaacga ttagccgaac atttaggttt
    12961 agcttttcag ataagagatg atctactcga tgtgattggt acaacgaaaa cattagggaa
    13021 aacagctggt aaagatgaac gtatggaaaa aaatacttac ccgcgtttat taggcctaga
    13081 aaagacacgt gaggcgttgg agattgagct gttatccgcc aacaagatca tagacaaatt
    13141 agaagaggac gttttttctt ttgatggcgg gctgatgcgc cagatgatca agcaatttga
    13201 tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag atgtattagc ggtcaaacaa
    13261 ggattatttg aaacaagaga acaagccaaa cgttcagtga tggctggttt gatttacaac
    13321 gaaaaaaacg agcggtttga taaaccggga gaaaaaattc cagtgagtag tgaattaaga
    13381 gtcaaaggta aaaaacttcc ttatgtttct cgagggggat taaaattaga aaaagcactc
    13441 aaagcatttg atctatctgt tgaaggaaaa acattgttag atattggtgc atccactggc
    13501 ggatttactg atgcagcttt gcaaaatggc gcaaaaatga gttatgcttt agacgttggc
    13561 tacaatcagc tagcttggaa aataagacaa gatcctagag tcgtcgtaat ggaaagagtg
    13621 aattttcgtt atgcaaaacc cgaagacttt accttaggaa ttccagaagt tgctgttatc
    13681 gatgtttctt ttatttcact aaaactgatg ctgcctccat tacatgctat tttaaaagaa
    13741 gagggagaag tgattgcttt aatcaaaccc caattcgaag caggcagaga agctgtaggg
    13801 aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag ttttagaaga cattttgtca
    13861 tttgcggcac aaggtggata tgatgtgctg gaattaagtt attctccgat cacgggggga
    13921 gaaggaaata tcgagttctt ggctcacttg aggaaagtac ccgaaagcgg aacaatcaat
    13981 tcagctatta atatggcaga agtggtttca aatgcacacg aacagtttga ccataaataa
    14041 attgtaactg tgaaaatcaa gtgagaagta tgcggattct tctttattat accaaggaaa
    14101 taagctatca ttgattaaga agaatatgct aaggagcgaa gacgtaatga gaaaaaaaga
    14161 tcggcatcgt ttgattactc gattgttgag tgagcaagat atccgaaagc aggaagaatt
    14221 tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa gctacgattt ccagagatat
    14281 aaaggaactg aaactgatca aggtaccggc ttcagatggc ggctaccgtt atagcatacc
    14341 agctgaaaca aacgaagatg taaatactaa attggaaaaa ctattaaaag acgctttcgt
    14401 ggcagttgac caaatggaaa aatttgttat tttgaaaaca ttgccgggaa atgcttcggc
    14461 agcagcaaat ttgattgata aacgttacaa gaaagaatta ttttcgatta tcaatgatga
    14521 cgataatgtt ttgatgatta cacgaactga agaccaggca gttttattga agaaagactt
    14581 cctgcattac ctttaagttg aggagcgaat gtgatgctac aagaaatcag tattactaac
    14641 tttgcgatta ttccggaatt gcggctttct tttcacgaag gaatgaccgc gctgaccgga
    14701 gagactggtg cgggtaaatc cattattatc gatgctttag gtctgttagc aggaggaaga
    14761 ggttcaagcg attatatacg tcaaggagca gaaaaatgtg tgcttgaagg gttgtttgaa
    14821 ctaccaaaac aagaaggatt ctctgaactg atggttgaat tagggatcga aacagatgag
    14881 gacaatctga ttgttcgacg ggatatgtcc ttaactggaa aaaatgtttg tcgagtgaat
    14941 ggtcatatta tcacattagc taatctgaga agaatcggca gttatttagt agatatccaa
    15001 ggacaaaacg aacatcagga actgctccag cctgaatcac atcttgcttt attggatcgt
    15061 tttggagatg tagctttcca acagaagaaa aagagttatc agcaagaata tatctcttat
    15121 cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg aaaaatccta tgttcagaga
    15181 atagatatgc tccatttcca gcaagaagaa atagctgctg ctgatttgca agtcggagaa
    15241 gaagagaaat taaaagaaga aagagaaaaa cttagcaatt atcaaaaaat tgctgatgga
    15301 ttagctgcag gttacggagc actgacgaat aatgaacaaa acagtgtaga tggtgttggc
    15361 ttggccgttt cagaactgca agggatcgcc catctagatg tggagtatga agcaatttat
    15421 gaaaatatcc aaagtgctta ctatttgctg caagatgcaa tcggcgatat gagtcgtcag
    15481 attgatttat tggagttaga tgagagtcgt ttagaagaag ttacgcaacg tttagaatta
    15541 atccgacagt tgaaacgtaa atacggcgaa tccattgagt caatattggc ttactatgac
    15601 gaaatcaccg aagagctagc ttcttctgat ttttcagaag gtcaattgga caaaatgaaa
    15661 gaggaactag agcaaaaaga gcttctactt cagcaacaag ccgcagatct tcatgaagcg
    15721 cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc atgaattgaa aagtctgtat
    15781 atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag aaaatagaca attgaatcat
    15841 gatggatttg accaaatcga attttacatt acgaccaatc caggagagcc actgaagcct
    15901 ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt tgcttgcttt gaaaactatt
    15961 ttttcttctg agcaaggtgt gacaagtatt atttttgatg aagtggatac aggtgtgagc
    16021 ggaagagttg cacaggccat cgcagataaa atttctcaaa tatcgaaata ctctcaagtt
    16081 ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg actatcaata ttatattgtg
    16141 aaagcagtga ttgatggacg tacgcaaact tctgtttcag agttgaaaac aaaagaaaga
    16201 gaagaagaaa ttgcacgtat gctggctgga agtgagatta caaaactcac actcgaacat
    16261 gcaaaagaac tattgaaact cgcaaaaagg acctgagcca cctcgcagtg gttcaggtcc
    16321 ttttcagcag ctgatagcta tattttattt ttacatgaca ctcattacag cagtcaatac
    16381 aacagatcca atcgttaaaa tcagcattgt ccaaatgaca aattttgtta ctttagcaaa
    16441 agtacttgtt tgtttttttt cacgcattgt tttcaacctc gtcattaatt agttgattca
    16501 agtgtacaat cttttttcca tttccacaag agggcttttt tataaaaact tccctttttt
    16561 taaataatag gtagttacga tacaaataaa taatgttaat agaactagcc atataaaggc
    16621 atcatccctt tcagcaatag gtaacttaat gttcattcca tagatactgc cgacgattgt
    16681 cggtatagtt aaaataatcg ttaaagaagt taaaattttc ataatattat tcagattatt
    16741 cgatacgatt gctgaaaaag tctgattcat ttgttctact agttttaatt gaatgcgaga
    16801 agtagtcatt gcctgctctg tttccaccaa tacatcacgt aaatgatttt tcaaagcatg
    16861 attttcttgg aaaagtttat tttgcgctag ttctttcaat gcatctaaat tagcagaagt
    16921 cgcttctctg aaataaacga ggcttttttg gatatccatg atttgataaa gttgtttgtt
    16981 ttcagtggat acttgaagtt tggcttccaa agtatcgcat tgagattgaa gcgtagctaa
    17041 ataactgttg taagagagtg tgacctgcca aatgaattga aagaagatat tgagattagg
    17101 cgaaatatca ctttttggaa aggtctggct aaagactttc tttaaaaaca gtggttcatg
    17161 attcgtaatt gtgataagct tctttttggg cgtcaaaata atagagagcg ggtatgtatc
    17221 cacttgaaaa taaccgctag gacttgtttt agcaaaagga tattgtaaca ataataacgc
    17281 tggctttgtc aactttgatt gattgaattc ttctgtacgg gaattttctg catcgtccaa
    17341 tatggaagtg atgtaatctg acggaatttc aaattgatgt gtcagctgat gaatctcttc
    17401 ttgattagga cgttctacac aataccagat cgtttggtca gtttcctttc cggtattacg
    17461 cagtttctca ttatctaatg taaaatattt gatcatgatt ttcctcctgt tgcctgtttc
    17521 ttttattata acggataggg aattggaata catggaatgt gtttgtaaga tatagaatat
    17581 aactgcataa atttaaataa aatgaaacag ttgtgtaaag tatatacagt atacttgaag
    17641 caacgataaa aaaggagttt acgttcatgg gcacattcac accacttatc ttgttttggt
    17701 atatttaccc ggtaatcgtt ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa
    17761 atcgtcgttt taaaataaaa gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc
    17821 atgcattgtc tagaaataca gggacaattt ctatcttacc gtttttcttt atttctgttt
    17881 tgctaatggg aatttgtatt gctgttttcc aagcatatta ttatgaagaa attatttatc
    17941 cgcgttattt caagatgact tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg
    18001 tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt
    18061 cttttaaaag gctttttgca gcttgatttt taaaaaaatg tttatgaata aaaaaagttg
    18121 ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac taattttagt
    18181 tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa tattgatgca
    18241 aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg
    18301 acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga aaatctggaa
    18361 gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt gcgattcttc
    18421 tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat acccagcacg
    18481 ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt
    18541 gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga gaactttgac
    18601 gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc tgactaatga
    18661 ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga ttgaacatca
    18721 aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt gaatatttat
    18781 taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg
    18841 atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca tttatcaaag
    18901 cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta gacggcattt
    18961 tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga tttagttatc
    19021 atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc gcttatcacg
    19081 taattaatga atattcctac catgaattag taaagatttt cttccgctat ggcgaggaga
    19141 aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa
    19201 caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca agaagaaaag
    19261 gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat gacgaactcg
    19321 gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat ggaagaatca
    19381 gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt aaagaatata
    19441 gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc caaccagaac
    19501 tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca gaaaacaatc
    19561 gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag gagtagaatg
    19621 atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt ggtggatcag
    19681 ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg
    19741 gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt
    19801 gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag cggagctgaa
    19861 aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa aacgagttta
    19921 gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc agaagaaaaa
    19981 ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct taaaaataaa
    20041 attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt
    20101 atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg tttctcgtat
    20161 atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa acagttgtac
    20221 caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg
    20281 ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac atatacctct
    20341 ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga aatcctgcat
    20401 aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa
    20461 gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga aaccaagcaa
    20521 aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt cgaccatcag
    20581 gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc agttccagac
    20641 aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa tgatatcttg
    20701 agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc tctgccaggt
    20761 acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac tttagacagc
    20821 agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata tcagccagaa
    20881 gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat gggacaacga
    20941 cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat ctggcgtaat
    21001 ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac tactgcagct
    21061 gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa aatccaagta
    21121 gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa
    21181 gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt aggcggacga
    21241 tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg attagatgat
    21301 gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag tgcttatgga
    21361 caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat tgcgaataac
    21421 ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac tggagaagag
    21481 cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac agaaaaagtc
    21541 cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat
    21601 agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc ttctgataca
    21661 gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat ggttccttct
    21721 gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata tgatcgaatg
    21781 gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa agaaactgaa
    21841 gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta tcgtaatctt
    21901 gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt
    21961 aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat ctctggagaa
    22021 aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg atggtcaaaa
    22081 gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga tggagatggt
    22141 tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa acttagtttt
    22201 actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga cacaagcatt
    22261 gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat ttattggtta
    22321 ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag
    22381 caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta ttttgacagg
    22441 aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct tgctttttgt
    22501 attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat tcaagaaaag
    22561 aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg gaatcatctt
    22621 ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg gactagatct
    22681 acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat tttcaaatgc
    22741 agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta tctcttttgc
    22801 agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca tttgcttaag
    22861 tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca agatttttat
    22921 gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta taatgctaaa
    22981 ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag caagtgtgat
    23041 gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt ctccaattca
    23101 tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt tttggttgat
    23161 cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga cgggaaatga
    23221 aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca aaaagcggag
    23281 taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat gatggaaaac
    23341 cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa gtaataacag
    23401 gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa aatccaggga
    23461 ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg gtaattacgg
    23521 aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca ggaacgaacg
    23581 gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca gatcaaggaa
    23641 ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat
    23701 cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc acagatttcc
    23761 gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat catggtacaa
    23821 gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa aaagattatc
    23881 tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca aaagcacgcg
    23941 tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac gattactcaa
    24001 tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca ggtaagcata
    24061 atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata tcaaatgaag
    24121 ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag
    24181 aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta gcgactaaaa
    24241 tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga ttagacagag
    24301 gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg gtcgtatttg
    24361 gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag acaatagtaa
    24421 cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca gatggtgatg
    24481 tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt gaaactagag
    24541 gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag cgagcgataa
    24601 aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc agctcttgcc
    24661 tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa
    24721 cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac cttggagatt
    24781 caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt gtttcttaag
    24841 agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt tatcggtact
    24901 ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat tccaacagtt
    24961 atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg atatgtggat
    25021 cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa agcagttctt
    25081 gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat tttgaaaaca
    25141 tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca aggagcactt
    25201 aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc ttaccaagtt
    25261 ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa tcctgaaata
    25321 caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc ttccagtgat
    25381 ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct ggggttaccg
    25441 gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa tgcaatgagt
    25501 cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg tgaatcacta
    25561 agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat gtgccgagct
    25621 tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt aaaacaaata
    25681 atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc
    25741 atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga aaatatctgg
    25801 cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta gatacacccc
    25861 tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa agccagtccg
    25921 agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga ccatataacg
    25981 gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc
    26041 ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc tattatgttt
    26101 ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc aacagccaag
    26161 aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag tattttcatc
    26221 gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca gcaaatatac
    26281 atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag attgctttga
    26341 tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta gatgaaaaag
    26401 tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct gcaaaaatca
    26461 aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca atttctgaaa
    26521 tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg aatgacggca
    26581 atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat tcgcaagtag
    26641 ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt
    26701 acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact gaaagtgaag
    26761 aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac
    26821 ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa
    26881 tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa aacaacttta
    26941 aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga agcagtgaat
    27001 atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta tatggaatag
    27061 taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg
    27121 aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga atattattgg
    27181 agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata tcgataaaac
    27241 ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta tccagatcaa
    27301 aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc agggaatgat
    27361 tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg tggcttcagc
    27421 tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt
    27481 tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg taagattaga
    27541 aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac gtaaatgcgt
    27601 ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc tgactgaatc
    27661 aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg gtggcggcca
    27721 aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg accaagaagg
    27781 tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta acaatgcaga
    27841 agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag atgaagaatt
    27901 tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct acttgtcaga
    27961 aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat tggaccaaat
    28021 cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg
    28081 tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca
    28141 catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt attcagcaaa
    28201 cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg
    28261 tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag cacctgaaga
    28321 aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa
    28381 gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat atggaattct
    28441 caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc ggtggtggcg
    28501 gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa ttcattacag
    28561 ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc caattaggcc
    28621 ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa aaagcggcag
    28681 aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt ttcatcactg
    28741 caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag
    28801 aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac
    28861 gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat acattattaa
    28921 ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg cttgaagctt
    28981 tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat ttgatcacag
    29041 cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa aaccaaggta
    29101 cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa
    29161 aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa gtacttctta
    29221 acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca gatatcgtag
    29281 caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat gaagaaatgg
    29341 gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca
    29401 agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag actgtattag
    29461 atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc gattgggata
    29521 tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac aatatcgaaa
    29581 aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa
    29641 atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat gattgctgac
    29701 aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca tcgtgatccg
    29761 aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg
    29821 gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa
    29881 caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt acagcgcaga
    29941 aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag tttaagatta
    30001 gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga agtaaatgtt
    30061 tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt tgtggaatct
    30121 ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc taaagaagct
    30181 tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga tcttgttcaa
    30241 gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag tcaagacttt
    30301 caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct attcaaagac
    30361 gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta attttttcgg
    30421 attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa aaaaggctgt
    30481 acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat ctgctgcgaa
    30541 tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa gtattcagcc
    30601 ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg tagcgaaaga
    30661 aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa aaatcactgt
    30721 aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca ttgctggaga
    30781 agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa tcgttgattt
    30841 tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag atgagatttt
    30901 tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt ttgcagatgc
    30961 gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact attacttcat
    31021 ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc attgctctct
    31081 tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat ctgcgaaccg
    31141 tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt cacaatttta
    31201 ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt tatatggata
    31261 actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa tagataggag
    31321 gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg tacatttatc
    31381 gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta tctgaccgaa
    31441 tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag cagtgatttg
    31501 cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt gattttcccc
    31561 gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat ccattatcct
    31621 agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg aacggggatc
    31681 aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt atttattgca
    31741 aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc
    31801 ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac acaagaaaga
    31861 ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa tatttccaga
    31921 cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac agaaaataca
    31981 agacctgatt ttgtacttga actattagat attatttcag tcagaggtta tggcagagtc
    32041 cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact gttcggtgta
    32101 ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta gatatccaaa
    32161 ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat gatttcttag
    32221 atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag
    32281 tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct ttgaaccaat
    32341 caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc aaggaatccg
    32401 aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac gctgaaaaac
    32461 gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa
    32521 cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa acacgagtat
    32581 tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa agtccggaat
    32641 gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa gtgatcaaag
    32701 aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca gacacaggag
    32761 tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg atcgtgcctg
    32821 gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc gagccgatga
    32881 gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga cctgcttttc
    32941 cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag tttgcactag
    33001 atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc
    33061 ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg aaagaaacct
    33121 tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga gaaggcgagt
    33181 ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg aacgaaggaa
    33241 aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc catttaggac
    33301 attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg tctggctatc
    33361 gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa gtattggcta
    33421 ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat
    33481 atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga gttgcaggag
    33541 attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa gtacgcgtat
    33601 ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt tactggtctc
    33661 cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta aaatctccat
    33721 ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa gatacagctt
    33781 ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc
    33841 caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt
    33901 tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta aaagaatttg
    33961 ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt gaatcacttg
    34021 tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat actgctccag
    34081 gacatgggga agatgactat atcgctggta gcaaatataa actatctgta gtgtcacctg
    34141 tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc ttttatgaca
    34201 aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg aagctagatt
    34261 tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa
    34321 ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat gaagtagaaa
    34381 aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg
    34441 gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt ttctatgctg
    34501 aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg
    34561 aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct gaaggattta
    34621 cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg gatgtctggt
    34681 ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta tcattcccag
    34741 cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca agtatcacga
    34801 ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa ggatttgctt
    34861 tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca ccagaaaaag
    34921 tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta gattatgaag
    34981 cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat cgtaaaatca
    35041 gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa aaagatactg
    35101 tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg aaccaagtaa
    35161 tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt taccgtacag
    35221 ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct aaagatgtag
    35281 tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa
    35341 cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca gaagaaatct
    35401 ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct ggttatgaag
    35461 agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac ttccgtgata
    35521 atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa tctttggaag
    35581 caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag
    35641 atattgcaca attattgatc gtttcaccag actactttga agttatggca gcagatgaac
    35701 aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa aaagcagacg
    35761 gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat gaaaaacttc
    35821 ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg
    35881 aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg tcacaccccc
    35941 ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg ttgccagatc
    36001 cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat tgatccagct
    36061 ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac atccacgata
    36121 tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc attcaacgca
    36181 tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc actatttcta
    36241 aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc attcaaggat
    36301 aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg aagtgtcgta
    36361 tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg tttgaatacg
    36421 atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca
    36481 cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc agtctctgat
    36541 tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc caattcacct
    36601 tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata aatgcggata
    36661 gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc tactggcggt
    36721 tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg
    36781 ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc aaaagtaatt
    36841 tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt ggcaaaacgg
    36901 atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct gaaaatatct
    36961 tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg gtaatcagaa
    37021 ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat attttgtgat
    37081 ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata atagacacgg
    37141 ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa tgtctgataa
    37201 tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa ggtagttgct
    37261 tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg gtagtgctca
    37321 tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa atttcctttt
    37381 ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg
    37441 aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt tatattttat
    37501 cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta tcattttgga
    37561 tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg ttgatattca
    37621 ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt gcgatacgca
    37681 cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg acaacttcct
    37741 cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa tagttttcta
    37801 aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca tcatggactt
    37861 cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa
    37921 tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga taaggggaat
    37981 gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat gcagctgaga
    38041 catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca cctagttcta
    38101 aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa
    38161 aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt ctaaataaaa
    38221 aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag aagactcatt
    38281 ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca gataacaact
    38341 tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa aagataagat
    38401 aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc acagatgtgc
    38461 caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt
    38521 gctggctatc actaaagaac catgtacctt gatactttga gagtccttct gacttttccg
    38581 cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt gaaccaagaa
    38641 aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc gcatagacat
    38701 attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta agactttcga
    38761 ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga agaaaggtga
    38821 cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc gaatcggttt
    38881 atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa aaatacaggt
    38941 agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc tcctggacct
    39001 aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg gtttaatatc
    39061 gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac gatcctttta
    39121 ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct tcactccaaa
    39181 tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg
    39241 acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag aggattgaaa
    39301 catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt cttgtttatt
    39361 gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc agcactagct
    39421 gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta
    39481 tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat cccttttgaa
    39541 gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat cggaacaaat
    39601 tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga attgacagcg
    39661 gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga aggggatatc
    39721 gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac catttatatt
    39781 gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc aggaaaaaat
    39841 gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat catccatttg
    39901 gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga atcaggtttt
    39961 aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa tttcagctat
    40021 atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa tatcttctcg
    40081 actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata tgcagctgtt
    40141 gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc tactggtgta
    40201 gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat ccagccaggt
    40261 aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac gctagtgatg
    40321 aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa agaaatcatt
    40381 gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga ttttgccaat
    40441 gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg gatcgagcta
    40501 gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat caaattcgat
    40561 gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc gaaaattggc
    40621 agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga gcagtttttc
    40681 agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca ggtaaaacac
    40741 tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt gcgttggtca
    40801 tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt gaatggggaa
    40861 aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa cgtcagattg
    40921 agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata ttggaattga
    40981 tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat gaagcagacc
    41041 agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa gctccaacag
    41101 agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa
    41161 tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct cgaaaaggac
    41221 tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg agacgtctca
    41281 cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa ttaggcgtaa
    41341 tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct gatcagaaca
    41401 agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga gcacttttga
    41461 ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta gtgaatgcag
    41521 aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt cgaatgggta
    41581 atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta aaacggcttt
    41641 tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag ctgcaaactg
    41701 agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg aaaacttttt
    41761 ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata
    41821 aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt tatgctatga
    41881 ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga
    41941 agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg atgtagctga
    42001 gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat acaaggccca
    42061 tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa
    42121 atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac tcgttggaga
    42181 aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt atccaccgcg
    42241 ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg
    42301 tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat ttgtttcttt
    42361 caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct ttgggatcaa
    42421 aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca aagcagacgg
    42481 cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg aaatgctgtt
    42541 tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag tacatgaagc
    42601 atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat ctacgttcaa
    42661 tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac tggatgagcg
    42721 ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga
    42781 tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa atatcggtca
    42841 agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc gtactcatca
    42901 tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa aaggattact
    42961 gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa gtcagtattt
    43021 gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac gaaacatgca
    43081 ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg tggagaacat
    43141 tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat atgtcaaaga
    43201 attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc ggttaaccaa
    43261 tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa aagctcgttt
    43321 tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt
    43381 ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg tagcaatcga
    43441 tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg aaatcggctt
    43501 tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag aaaacaaagt
    43561 agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg agatggcaca
    43621 agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta tggaagttca
    43681 aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt tgacgaatgg
    43741 cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga tttatgaaga
    43801 gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga tagaagcgat
    43861 acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc agctttcctt
    43921 tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa
    43981 agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat atcagcgtat
    44041 tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt tagaacgtga
    44101 atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag caattgcggg
    44161 aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg tttttgatga
    44221 tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag ctaaaattgt
    44281 cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga tccgttcatt
    44341 gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac ctggagtaga
    44401 tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac aagaaagcca
    44461 tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa tggatatcgt
    44521 tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag aagatgatat
    44581 cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac actaatcata
    44641 ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac
    44701 gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa
    44761 taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca gcagggatga
    44821 gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg
    44881 atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag aatgtcgatg
    44941 tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa aaacttgcgc
    45001 ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg aacggagaaa
    45061 aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt gggggtataa
    45121 caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt ttggcggtaa
    45181 tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt ttgagttagc
    45241 ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg cacaaacaga
    45301 catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga ctgttcacag
    45361 tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa tcatcgaact
    45421 ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag aagaaaaatt
    45481 tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa aacttatagg
    45541 aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct gccggttgcc
    45601 gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat cggcacactg
    45661 cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg tgatttaccg
    45721 caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat ctttgccgta
    45781 ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc aatcgccgga
    45841 ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt aaatgatcta
    45901 gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg tccagcatta
    45961 tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa tagttctgag
    46021 attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg gctaaaacta
    46081 gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat ttcagtcatt
    46141 attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc agtaccacca
    46201 gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata cgtcattgct
    46261 attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga tttggtgcaa
    46321 aaatatattg cagagccatt cctaggatta tctcaagggt tgggagcagt attgatcgtc
    46381 acagtatttg ttcaagtatt ctggttcttt ggtattcatg gaccaaatgt attggcacca
    46441 gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt
    46501 tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg aaaagcttat
    46561 atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg cggaacgatc
    46621 gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac agtagctaaa
    46681 ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg tctgccaatc
    46741 gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc aacagcaatt
    46801 ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt cacatgggta
    46861 gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc accgatcgta
    46921 acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat ctctgcaaac
    46981 aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg ggacgcttta
    47041 agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa ttgccagtgc
    47101 aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag gaatgacttt
    47161 tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag aagatccgga
    47221 agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa cagtccttta
    47281 ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg agatgataaa
    47341 aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt tgaatccaca
    47401 ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag gattaggaat
    47461 ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata actaatggaa
    47521 atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat ggactctgtc
    47581 cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg
    47641 gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga agaagtcatt
    47701 gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt cgttcattat
    47761 caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa aatggagtct
    47821 tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat gtattttaaa
    47881 cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat caatgaaaca
    47941 tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca taggaagata
    48001 gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa atgacaaaca
    48061 aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc acttatgcat
    48121 ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg gagaattctt
    48181 ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg gcgacccctt
    48241 ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat gttgcttctt
    48301 atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc cttgaagatt
    48361 gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc ttctatcggc
    48421 gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt cgtgatgtgc
    48481 cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg
    48541 caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg aagcgcgaag
    48601 actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg
    48661 atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct tcctttatta
    48721 ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt
    48781 ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt aatgatcaaa
    48841 attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg atggcgagga
    48901 tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa gctgcatttg
    48961 ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg gaagtaacaa
    49021 tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca atcgttcgag
    49081 gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa gagttggtat
    49141 tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca atcttgccac
    49201 agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac aatatttttg
    49261 tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta aatcctcttt
    49321 tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt tgtggtcttg
    49381 gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc gttctgcgta
    49441 tactttttct tttggcttat cattcattgg aaaggacctc cctacattat ttcattctac
    49501 ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta taagctgatt
    49561 tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt tattgaaatt
    49621 gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg ctggttcaat
    49681 tccagtcagg aacgtacaaa taacccctgt atcctttgtg atacaggggt ttgttttgtg
    49741 tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg aacacagcat
    49801 tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa gtagacattg
    49861 tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat
    49921 aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc
    49981 ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa actaatccat
    50041 tttttaagtt gaaaaaaaca aaattatgag ggtttttaac ttcaaaattc tcgaataatt
    50101 ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca atggaaattt
    50161 tcctatttga agtttcgttt tttgtttttc cccattcatt taatttataa ttccatgttt
    50221 tattaatagt gatgatttga tcttcgaaat cgatatcttc ccatgttaat cctagaagtt
    50281 cagcaaatcg cattccagta gcaccagcaa ctaacacgag cataggagaa gagtactgcg
    50341 cagaaaggtt ttcttctacg agtttcatta aagatttaaa ttgatcataa tctaaatatt
    50401 tatcttcttt tggcttcaat gaatcttttc ctttgattac tgcttttcta gttggatcaa
    50461 acggaattaa tccttcttct acggcatctt tcaacgatgc tctaatatgg ttattaaact
    50521 taataacggt ggattttaca tggtctttcg catacttatt taaaaagcgt tgatatccaa
    50581 ctctatctaa atcagaaatc aataccgctg gcatatattt ctttatgttc attaacgtat
    50641 cttcgtattt tctataagtg ataggagata ctgttccttc tttataaagt tgcatccaat
    50701 cctcaaagta atctgataat aatagatttt tgcgatcccc tttaaggcct ttgttcaagt
    50761 tatattctaa ttcgttggcg gcatcttttg cttcgccttt tgtcttaaat cctgattttc
    50821 ttatcttcgt gtattttccg tcatcttttt tgtatgaaat ttcgtattgc caactatttc
    50881 cacgtttgac taatcttgcc ataattgaat ctactctctt tctctgatac aataggcact
    50941 ataaagaagc ctactgtata ggtttgtttt tcttagaaca cgcttgcttt ggacggtggg
    51001 cgtgtttttt atttattata cagttgcttt aaaaatagct gctttttcaa atgatactaa
    51061 aggagagaag tggatttcta tttctccctt ttggtttaac gtgaaatgag cagttacatc
    51121 catttgtttt cctggagcaa cagatcccat tgtattttcg ttggcatatg tctctgattt
    51181 tttgtcatct ggtccatata cttccacatc tgtacctaca gggatatctg aatcgccatc
    51241 attttttacg gtataagtaa tttttactac ttgtgcaggt tggttttctt caaattgatt
    51301 tctttcatca gttagttcta cactatttag tgtatattca gcatcaccaa cttttacagt
    51361 gtcaccaatc ttatagaagg tatcgctttt ttcttcttta gaagatgaag cagaggttga
    51421 agatttagtt actttttcgc caccattatc acttgcttta ttactatctg atccaccatt
    51481 taaagcagaa ccaataatta taattaaaat tactgctaat acccaaaacc atactttttt
    51541 gtaaaaaggt ttactaactt tatacatttt tccgtcttga cccataactt tttttgccat
    51601 ttaaatattc ctcacttctt gttataatat atttgcgatc tcagaaatga ggtatgagtc
    51661 cgtgtgccag cacgggcttt tttaatgttt tgctgaaatc ggttttttta ataattcttg
    51721 atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc attggcagac
    51781 agcataacaa gtgtatctga gcttattctg aatagaataa tactccataa atttttcgag
    51841 attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata aaattagaat
    51901 agctatttta tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa
    51961 aactgaatat tcaaaatgag aagcggtgaa atg
//
LOCUS       DACDSR010000009        51816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDSR010000009 DACDSR010000000
VERSION     DACDSR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.009"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..729
                     /locus_tag="IWK15_000574"
     CDS             <1..729
                     /locus_tag="IWK15_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546203.1"
                     /translation="AELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFG
                     KPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYV
                     NGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKK
                     TSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQ
                     KNEFMQFGLTVLGICTFIITVINR"
     gene            complement(969..1052)
                     /locus_tag="IWK15_000575"
     tRNA            complement(969..1052)
                     /locus_tag="IWK15_000575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1016..1018),aa:Leu,seq:gag)
     gene            1435..2652
                     /locus_tag="IWK15_000576"
     CDS             1435..2652
                     /locus_tag="IWK15_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9546204.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2836..3672
                     /locus_tag="IWK15_000577"
     CDS             2836..3672
                     /locus_tag="IWK15_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9546205.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3748..4896
                     /gene="nagA"
                     /locus_tag="IWK15_000578"
     CDS             3748..4896
                     /gene="nagA"
                     /locus_tag="IWK15_000578"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9546206.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5116..6174
                     /locus_tag="IWK15_000579"
     CDS             5116..6174
                     /locus_tag="IWK15_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9546207.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6193..7212
                     /locus_tag="IWK15_000580"
     CDS             6193..7212
                     /locus_tag="IWK15_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9546208.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7314..8030)
                     /locus_tag="IWK15_000581"
     CDS             complement(7314..8030)
                     /locus_tag="IWK15_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9546209.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7999..8784)
                     /locus_tag="IWK15_000582"
     CDS             complement(7999..8784)
                     /locus_tag="IWK15_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9546210.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8775..9524)
                     /locus_tag="IWK15_000583"
     CDS             complement(8775..9524)
                     /locus_tag="IWK15_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9546211.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9822..10346
                     /locus_tag="IWK15_000584"
     CDS             9822..10346
                     /locus_tag="IWK15_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546212.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10362..11861
                     /locus_tag="IWK15_000585"
     CDS             10362..11861
                     /locus_tag="IWK15_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9546213.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11949..12551)
                     /locus_tag="IWK15_000586"
     CDS             complement(11949..12551)
                     /locus_tag="IWK15_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9546214.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12655..13611
                     /locus_tag="IWK15_000587"
     CDS             12655..13611
                     /locus_tag="IWK15_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9546215.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13916..14872)
                     /locus_tag="IWK15_000588"
     CDS             complement(13916..14872)
                     /locus_tag="IWK15_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9546216.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15046..15606
                     /locus_tag="IWK15_000589"
     CDS             15046..15606
                     /locus_tag="IWK15_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546217.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15599..16423
                     /locus_tag="IWK15_000590"
     CDS             15599..16423
                     /locus_tag="IWK15_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9546218.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16433..17050
                     /locus_tag="IWK15_000591"
     CDS             16433..17050
                     /locus_tag="IWK15_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9546219.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17068..17751
                     /locus_tag="IWK15_000592"
     CDS             17068..17751
                     /locus_tag="IWK15_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9546220.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18434..19669
                     /locus_tag="IWK15_000593"
                     /pseudo
     CDS             18434..19669
                     /locus_tag="IWK15_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19677..20564
                     /locus_tag="IWK15_000594"
     CDS             19677..20564
                     /locus_tag="IWK15_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546221.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20581..21417
                     /locus_tag="IWK15_000595"
     CDS             20581..21417
                     /locus_tag="IWK15_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9546222.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21420..22043
                     /locus_tag="IWK15_000596"
     CDS             21420..22043
                     /locus_tag="IWK15_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546223.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22040..23332
                     /locus_tag="IWK15_000597"
     CDS             22040..23332
                     /locus_tag="IWK15_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9546224.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23691..23788
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23904..24485
                     /locus_tag="IWK15_000598"
     CDS             23904..24485
                     /locus_tag="IWK15_000598"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9546225.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24488..25795
                     /locus_tag="IWK15_000599"
     CDS             24488..25795
                     /locus_tag="IWK15_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9546226.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26088..26576
                     /gene="purE"
                     /locus_tag="IWK15_000600"
     CDS             26088..26576
                     /gene="purE"
                     /locus_tag="IWK15_000600"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9546227.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26560..27693
                     /gene="purK"
                     /locus_tag="IWK15_000601"
     CDS             26560..27693
                     /gene="purK"
                     /locus_tag="IWK15_000601"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9546228.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27706..29001
                     /gene="purB"
                     /locus_tag="IWK15_000602"
     CDS             27706..29001
                     /gene="purB"
                     /locus_tag="IWK15_000602"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9546229.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29181..29558)
                     /locus_tag="IWK15_000603"
     CDS             complement(29181..29558)
                     /locus_tag="IWK15_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546230.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29814..30542
                     /locus_tag="IWK15_000604"
     CDS             29814..30542
                     /locus_tag="IWK15_000604"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9546231.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30542..30796
                     /gene="purS"
                     /locus_tag="IWK15_000605"
     CDS             30542..30796
                     /gene="purS"
                     /locus_tag="IWK15_000605"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9546232.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30798..31469
                     /gene="purQ"
                     /locus_tag="IWK15_000606"
     CDS             30798..31469
                     /gene="purQ"
                     /locus_tag="IWK15_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9546233.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31470..33692
                     /gene="purL"
                     /locus_tag="IWK15_000607"
     CDS             31470..33692
                     /gene="purL"
                     /locus_tag="IWK15_000607"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9546234.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33677..35116
                     /locus_tag="IWK15_000608"
     CDS             33677..35116
                     /locus_tag="IWK15_000608"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9546235.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35148..36191
                     /locus_tag="IWK15_000609"
     CDS             35148..36191
                     /locus_tag="IWK15_000609"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9546236.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36188..36766
                     /gene="purN"
                     /locus_tag="IWK15_000610"
     CDS             36188..36766
                     /gene="purN"
                     /locus_tag="IWK15_000610"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9546237.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36763..38298
                     /gene="purH"
                     /locus_tag="IWK15_000611"
     CDS             36763..38298
                     /gene="purH"
                     /locus_tag="IWK15_000611"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9546238.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38314..39588
                     /gene="purD"
                     /locus_tag="IWK15_000612"
     CDS             38314..39588
                     /gene="purD"
                     /locus_tag="IWK15_000612"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9546239.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39710..40624
                     /locus_tag="IWK15_000613"
     CDS             39710..40624
                     /locus_tag="IWK15_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9546240.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40757..42322)
                     /locus_tag="IWK15_000614"
     CDS             complement(40757..42322)
                     /locus_tag="IWK15_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9546241.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42315..43766)
                     /locus_tag="IWK15_000615"
     CDS             complement(42315..43766)
                     /locus_tag="IWK15_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9546242.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43780..44796)
                     /locus_tag="IWK15_000616"
     CDS             complement(43780..44796)
                     /locus_tag="IWK15_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9546243.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45016..46104
                     /locus_tag="IWK15_000617"
     CDS             45016..46104
                     /locus_tag="IWK15_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9546244.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46158..47684
                     /locus_tag="IWK15_000618"
     CDS             46158..47684
                     /locus_tag="IWK15_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9546245.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47686..48891
                     /locus_tag="IWK15_000619"
     CDS             47686..48891
                     /locus_tag="IWK15_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546246.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49148..50125
                     /gene="guaC"
                     /locus_tag="IWK15_000620"
     CDS             49148..50125
                     /gene="guaC"
                     /locus_tag="IWK15_000620"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9546247.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50389..51813
                     /locus_tag="IWK15_000621"
     CDS             50389..51813
                     /locus_tag="IWK15_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9546248.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT        16522 a         8834 c        11357 g        15103 t
ORIGIN      
        1 gcagaattaa ttttctcttt gacaactcca gggaatgcat tacgtgctgc acatgaaatt
       61 aataaatggt ttcctgaata taaaaatttc aactttctta ataagttaga tttaggtatt
      121 tcatcatttg gaaagccgtt ttttttagac atgaatatct tatttttatt gctatttttc
      181 ttaattttcc ttctaacata taggaaatgt caaaactatt atgtgcgtat attgactgca
      241 ttaccgtttt tcttaaacct gattatttat tttggtaata caatgggtca aagctttact
      301 tacgtcaatg gaaataaaag ggcaatgatt tggagtagta gtaacctgaa taatctcttt
      361 acagagttag gaacgaaact gtcacttttt tatccaggta cttggatagc tactttagta
      421 gtcttggcat tattactatg tttaattgtt ggaatttatt taagttttga caataagaaa
      481 acttctattt ttttagttat tttgatgata atgggatttt gttcaaggtt aattatggga
      541 ttttctccca cagtatgggc ttccggaatg cggacgtact atatacttta tgttgttatc
      601 gccatcttag tactaatggc agttaaagag ctaatgaaaa gcatgagtgt acaaaaaaat
      661 gaatttatgc aatttggatt aaccgtgctt ggtatttgta cctttataat aacggtgata
      721 aacagataat atacaaaaaa tccctcgctt tagtattagg tgagggattt tactcgtttt
      781 tcgattaatt gttttaacac ttgcaagttt ctacgagtag cttgtttccg accttcatat
      841 actcacctca ggctgtttac atgattgtac gttctttttg taaaggatac agtgatatgt
      901 atataatggc ataaagaaac gttcaaacaa aaaaactcgt gaagcaaaat taacttcacg
      961 agttgtagtg cagatgatgg gactcgaacc cacacgagct aacgctcaca tgaccctcaa
     1021 tcatgcttgt ctgccaattc caacacatct gcgtaataat aacacactaa tgatagcgag
     1081 gttttacata gttgtcaagc tcaaaaatgg gaggacttat taagatggag aaatatgacg
     1141 gagaattttc aggactggga atgatcttag gtgtattgat tggacttgca ttcggacgtt
     1201 ttttgcttgg ttttatgcta ggaatcattt gtggaattgc aatggattgg gcagcgaatc
     1261 tatggaatga ctatcatgac aattaagttc tcaacgcttg tttgtgtttt tttaatactt
     1321 acgtttttcc taaataagtg gcaaaatttt ctaggatgga gtatgatatt gaaatagtaa
     1381 ataacaaaaa atttggaaag ttctaacttt ttgaaatgag ggattggcta agtgatgaga
     1441 gaatttgaaa aatccaacaa attagaaggt gtcagttacg atgttcgcgg tcccgttcta
     1501 gaagaagcgg agcggatgca agaagagggc attagtattt taaaattgaa cacgggaaat
     1561 cccgcacctt ttggatttga agcaccaaac gaagtcattc gtgatttgat catgaatgcc
     1621 cgtgactcag aaggctactc agattccaag gggatctttt ctgctagaaa agcaattgag
     1681 caatattgcc aactgaaaca ttttccgaat gtgacgatca atgatatcta caccggcaac
     1741 ggagtgagcg aattgatcac gatgtgtatg caggggttgc tggacaatgg agatgaagta
     1801 ctggttccaa tgccggacta tccgttgtgg acggcttcta ttgcattagc tggaggcaca
     1861 cctgttcatt atatttgcga tgaagaggca gaatggtatc ctgatattga tgatatcaaa
     1921 tcaaaaataa cgagtcggac aaaagctatt gttatcatta atcctaataa tcctactggg
     1981 gcactttatc caaaagaatt attagaagaa atcgttgaag tagctcgaca aaataacctg
     2041 attatttatt ccgacgaaat ctatgatcgc ttagtgatgg atggtctaga acatattccg
     2101 attgctacat tagcgcctga tttgttcgtt gttacgctga acggtctatc caaatctcat
     2161 cgtgtagcag gatttcgagt aggctggatg gtcctaagtg gagataagtc acacgtcaaa
     2221 ggctatatag aagggctgaa tatgttgtcc tccatgcgtt tgtgttccaa tgtattgtct
     2281 cagcaaatta tccaaaccgc gctaggcggt tatcaaagtg tcgatgagct gctcctgcca
     2341 ggcggaagga tctatgaaca aagagaatat atctataatg caatcaatga tattcctggt
     2401 ctttcagccg taaaaccaaa agctgctttt tatatattcc caaaaattga tacaaagcga
     2461 ttcaatattt tggatgatga aaaattcgtt ttggactttt tacatgaaca tcatattcta
     2521 cttgtacatg gtggtggatt taattggaac cagccagatc attttcgaat cgtttatctt
     2581 cctaaaatgg atgatttgaa acaaacagct aagaaaatga gagaattcct agcaacctat
     2641 cgccaaaaat gattttaaaa accaaatttt tcagtaggag ggccacagac aaacacactg
     2701 aggctctctt tctagtggag ttttcttaaa taactaaagt atattattta gttgcaagtc
     2761 ttttcttgaa gtttgcgact tcctaaagta aaatacgaat agaatgcgaa tacgcatatt
     2821 ttggggagga tacttatgga aacgttaatc gagtcctttg gaacgatcat taaagagatt
     2881 cgaaaagaac aaaaaatgac gcaaaaaatg ctttcgcatg atatttgttc acaaagtgta
     2941 ttaagtcgga tcgaaaataa tgaggaactg ccgaatgtat tggtgatgat gcagatttgt
     3001 caacggctag gggtaacgat tgatcatgtg atgaatctct ctcgaaaaac tactcggaca
     3061 aacgataaat tatttgaact gttggatagt tgtttccaaa aacgtgatta tcggaagtta
     3121 gaacaagtac taaaatcttc tgatatttct gaaaatctgt atcaagcaac tgactttcaa
     3181 cgctattatt attatctcgg tgtttgcgaa ttcacgttgc gtcaaaatat ctctcagtcc
     3241 ctttattatt tgaaagaagc attaagctac tctagccaaa aagaaagaat ccatgtttct
     3301 gacacagaaa tacaactgat tagttgtatc ggaaaaatat atggtgtggc aggaaaaacg
     3361 gctgaagcac gctactattt gagtcgaagc atccaactgc tgcatcaatc agctgatgaa
     3421 cgatcaaaaa gtgaattttc gcaaattttt tataattatg gaaatttctt attccatcaa
     3481 aatgaaatcg acgaagcgtt agaacaggtg aatcaaggaa tctattgggc tcaagagaag
     3541 aatagctact attacctcaa tgatttattt gtgcttaaaa gcttaatata caagcgtaaa
     3601 gaagaaccag aaagagcagc attctatgaa ggcttagcac aagctgtgaa acaaatttca
     3661 aaaaatttgt aaatggtgta gaccattctt gacttcttct ctaaataaga gtactttaga
     3721 ggtagaaaaa caaagggaga gaatagaatg agaactttta tctatgctga caaattcttt
     3781 ttagcgtcag gtgtcaaaca tgcaggctat cttgaaatta ccgatggact ttttggtgaa
     3841 tacaccaaag aactacctga gggagaagtc aacatcattg accaaagcgg caaatggatt
     3901 gctcctggtt tggtagatac acatattcac ggctatatga atcatgatgt aatggataat
     3961 gatgcagaag gcatcaaagt gatgtctgaa ggtctgttat cttgtggagt gacttcattt
     4021 ttaccaacaa cattgacgtc aagcaaagaa cgtttaaaag atgtagctga gacaatcggc
     4081 aaaatgtatc aggaagcacc aggagctaaa atcaaaggaa tctattttga aggtcctttc
     4141 ttcacagaag aacacaaagg ggcacaaaac ccaagttatt tcggggatcc tgatttggat
     4201 actttccatg aatggcaaga agcttcaggt ggtttaatca aaaaaattgc tttagcaccg
     4261 gaacgtcaag gtgtagaaga gtttgtcaaa caagtgactg aagaaggagt agttgtttct
     4321 ctaggacaca gtaatgctac actagaagaa gcgcaaaaag ctgttgaagc aggcgcaagt
     4381 gtatttgtcc atgctttcaa tggtatgcgt ggattgaatc atcgcgaacc aggtatggtg
     4441 ggtgcgttat tatctttaga acatgtcttt tctgaattga tttgtgatgg ccatcatgta
     4501 catccaaatg ctgcgggaat cctaatggaa aaagcaggac atgatcatgt cgcattaatt
     4561 acagactgta tgatggccgg tgggatgcct gatggaaatt acaatttagg cgaatttcca
     4621 gtagtagtaa aagaaggaac agcacgtcta gaatctggca acttagctgg aagtatattg
     4681 aaattgaaag aagcaattaa aaatgttgta gactggggat tagcaacacc agaacaagca
     4741 gtcatgatgg ctagtctgat tccagcaatc agctgtaaaa tcgatgatac atgtggaaaa
     4801 atcgctcaag gacgcgatgc agactttatc gtattgaacc ctgatatgac attggaagca
     4861 acttatctag atggcgtgga acgtttccac gcataatata atttaagtta accttaaaac
     4921 taaacttctt aagtttggtt ttaaggtttt tttgtgttct agtaaaaatc actttacata
     4981 agtttcaaaa gtttctttaa tttcctattg ttcaataagt aacaatgtaa agataaaatt
     5041 aaaaatgtta atacgtatta gcaaaataaa agcttttttt attgtgaata aatgaacata
     5101 attaggagga gaaagatgaa cttatttgac atttttcgat cgactttgac agatatgtcg
     5161 attgtcagtg caatcacttc aacagttttt attatattat tagggtttgt ttgtagaaaa
     5221 aaggggattt tttctgaatc tgttggaaaa attctatcga aagtcgtctt aacagtggct
     5281 ttgccagcac tggcatttcg agctttcatg caagatatca atacagaatt actgatgcaa
     5341 ggaatcaatg tcttgatttg gggattagtt atttatatta ttcttattct agtttcaaaa
     5401 ccttttttct taagctataa aggggataag caaacaacgt tgcgtgtcct gacgatcttt
     5461 ggttctacga cctttttcgg aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg
     5521 atgtattcct caattttcaa tattggctac cgaatctttt tgtattctta tggatacata
     5581 aaaatgagtg gcttgaagat ggaagctaaa aatatcaaag cgatgttcgt caatccaatc
     5641 attatcgcta cttttgccgg ccttttcatt tggctgttcc aagggtatct gccacaagtg
     5701 tcagttacag gtgcagaagg agagataagc caagtagcgt ttttgcgaat tgaccagact
     5761 gcaccgtggc tatttcagcc gatggattat ttagctggtt taagttcacc attggcgtgg
     5821 ctagcaatcg gcgcaacatt aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact
     5881 tcatggtatt acagtctagt caaagtggta tttgtacctg cattgaatat cgtgattcta
     5941 gcagttttga cggcaacgaa tattctacca gtcaattttg aagcactggc aacaatcgtt
     6001 atcatgatgg ctacaccagc tgcaacagtg gcagcagcct atgctatcag ttttgatagg
     6061 gaggcacttt tagcctccaa tgcatcctta ctgtcaacta tttttgctgt ggtactgaca
     6121 cctgtatgga tcgttgtatt acaaatgatt catggcatag gtttattttc ataagaaatg
     6181 gaggaagaaa gtatgagaat cgcatgttac ggtgttcgtc cgaacgagat agattttttt
     6241 gaaaaattga atatttacca ttatgatctc agtctttatg aagaactgtt gacacatgac
     6301 aatatcgtgt cagcaaagga ccatgatgct gtattgttga ggggaaattg tatagcagat
     6361 gagaccaatc ttgcaaaaat gcaggaatat gggattcgtt atgtttttac acggactgtg
     6421 ggagtcaatc atatcgatct acaagcggca gctgattttg gtatgacagt agcccgtgta
     6481 ccatcgtatt cgccaaatgc gatagcagag ttatctttga cttttgccat gatgcttttg
     6541 agaaatactg cgtatacgac gatccgaaca tctttcaaag attttcgagt ggatgagcaa
     6601 atgtttagtc gtgaaatcag aaattgcaca gtagggatca taggaacagg caggataggg
     6661 ttgacggaag ctgttttgtt taagggacta ggagccaaag tgatcggcta cgatattcat
     6721 cagacagaag ctgctaaaaa agtattgacg ttccactcgc ttgaagagtt attagccaaa
     6781 agcgatattg tcagtattca tgtacctcat atcccaggtg aaaacgatca aatgatcaat
     6841 gaaggatttt tagctcaaat gaaaaaaggt agcatcttga ttaatacagc ccgtggagag
     6901 cttcaagata atgaagctat tttaagagct ttgaaaagca atcatttagc tggttttgca
     6961 accgatgttt ttgcaaatga aacagaggtt tttttccgat ccttcaagcc atgggagacg
     7021 attccagacc ctgctatcca gcaattagta gaaatgtacc ctagagttct tgtaaccccg
     7081 catgtaggtt caaatacaga tgaggcatta tcaaatatga tttcgacaag tttcgagaac
     7141 tttcgtgaaa taatagaaac tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt
     7201 caattaagat aagaaaacat ggatattcgt caataaaatg tttaaaaata gtccaagtaa
     7261 acagttgtta cttctgtata cttggactat tttgtatggg ataattcgat gactcaacag
     7321 taatctgtga gctttttccg cccattcttt gtcaattcat aacaggttag cggctggccg
     7381 atttccaaat atttgattct ttcgttcaat atatcttgtt ctactaaaaa attcaaatat
     7441 ttcttgacgg atatacgtga cagtgagctt ttttgagcca gttcttttgt tgaaaaaata
     7501 gtatcttctt ttagaataaa tgcacttatt ttttttaaag tcagttttga cagaccttta
     7561 ggcaaatcga tgaatgatgg tatttcgtga atgggagtgg tgtggttgaa tacttgatcg
     7621 atcaatttct gatcggcttt ttcagtcgtt tggattgtct gagagaaagc aagatacttt
     7681 tcgatcgcca gctgaaaacg ttgcaaagtg aacggtttga tcaaataatc taaggcaccg
     7741 tagcggatca cattctttat ggtttcgata tcatctgcag cagtgatcaa aatcactggg
     7801 atatcatttc cttgctgtct aagccaagat aaaaaatgga taccactttc gccaggcatg
     7861 tatacatcaa gcagcagcag atctgcgttt ttttgtctga aatatttttt tccttcttct
     7921 acatttctga aactttcgat tacgttcata ttagggatgc tttccacaaa agtacgattc
     7981 aattctgcaa ccattggatc atcttcgatt atcaggacat ttgtcatcat tttgatcttc
     8041 cttttttggt aattctagat agacgattgt accgccattt gtttgtttgg aaaattctaa
     8101 aaatccaccg tggcgtttga cgattgaatg gatgagattc aatccaaacc cacgattgat
     8161 tcccttcgtc gaaaaaccta gcgtaaatat tttttctgag atttcttctg atatcccgac
     8221 accgccatcg cagacggtca cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat
     8281 ccggacctgt ccgtttcttt catgtttttg tagcacggct tcttttgcat tatcaagtaa
     8341 gttccctaac acttgcaaaa gtgaatggat ggagtcatcc atctccattt tagggataaa
     8401 agaatcgctt tctaacagca tttcaacaga ttgttctttg gcttcgttta ttttgcctaa
     8461 taaaaaaccg gcaattgcag gtactttgat cagacttgag acttggccaa tctctttttt
     8521 ataattttga tttagctgct ggataaaatg ggtgacttct tcatacttct tcaaatcaat
     8581 caaaccagaa atgacatgaa gtttattcat gaattcatgg gtttgagctc tcatggagtt
     8641 tatatattgt ttcgtccccc caagctcctc cattaatttc attacttctg atcgatttcg
     8701 taaagttgcg actgctccta aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat
     8761 agtttctatc ccatttaaat acatggattg agtccgaacg gtcgtttgat gctcaatcgt
     8821 ctctttaaac aaaacgtcat atagattatc actgattttt ttcctacttc gaaatccaat
     8881 agctgctctg tttggtagaa aatgtttttt gcaggtgtat tgatcaagag gatagtttta
     8941 tcagaagaga tggctaagat tccttcatct acttcatttt cgataatcgt tttttcactt
     9001 aactgttctg ctatttcttg tggttcaagt ccaagcagta cttttttcat tttttgggca
     9061 agaaaaacgg cgcctatgat gcctacgcta agtccaataa tcaatgaaaa aacaatcatt
     9121 ttttgtccat ctttgacttg attgtcaatc gtgttgtttg ttaagccaac acaaactgca
     9181 ccaataatct cttgatcata ataaataggg acgaaatagc ggaagccgaa ccctaacatt
     9241 ccttcttttt cagagaaaaa ttcttgtcct ccaatcactt ttttcacatc ttcatgactt
     9301 gagaaaggaa gaccgatgta acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc
     9361 atgacaacga taaaatctaa attcgatgca atcatgattt catttgttat tccttgaatc
     9421 gcatgtgtcg ggtcaccagt ctgcagcttt ttttggatga gtttatcatt ggcaacgatt
     9481 ttagcaatgc tagagatttt atctgacaca ctagatcggt tcatcgaaag aataaaattc
     9541 ctgatcaata tggcagaaac aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg
     9601 atcgtgttct ggagctttaa tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa
     9661 agaatttatt gtgaaaattt taacatgaaa acaaaaattt aacaaattgt caacgataaa
     9721 cacaaccttc ctataaaaaa caccactgag tcggtaaaag aagctataca gaattttcaa
     9781 acattggtat gatagacgta gaaatggaag gaggcgtgta tatgataggg ggaatcgcac
     9841 ttgagacgat ttattggtat acattgctga tttgtgcagg attggcagtc ctattgatta
     9901 tttttggaga tgtattcgat tttgatggcc cgcttgatcc gatgcttatt atcccttggt
     9961 taactttcac aagcttattt ggttttctag gtgagcgttt aacagatcaa tcatctttat
    10021 ttttatttat tgctagtgga ggcatatcga tagtgttggt atttttgctt aatttctata
    10081 tattgatgcc gatgaaacat gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag
    10141 gacaagtagc tgttgtgatc acaccgatcc ctgtacgagg tatgggagaa attcagttga
    10201 aaagtgttac cggttccatt agtcgtccag cttgttttta cagcccccaa gaacagacag
    10261 ctgagcgggg aacgaaagta ttgatcattg agatcaagga aagaatctgt tatgtcatgc
    10321 cttatgaaag catgataaaa gcttaaagga gcgtgtgtta aatggaaata ccagttgttt
    10381 ttatcgcgat cgcaatcgca gctttgattt tactgatgtt gctgatcgtc tttgtagcga
    10441 agtaccagac agcaaaaccg gatgaagcgt tgatcatcag tggaagttat ttaggaagca
    10501 aaaatgtgca cattgatgaa agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat
    10561 tcgtcttgcc agtttttcaa cgttcgaatc ggatcagtct attatcgagt aaattggacg
    10621 tctccacccc tgaagtgtat accgaacaag gcgtaccggt gatgtgtgat ggtacttcga
    10681 tcatcaaaat cggttcatct gtagaagaga ttgctacagc agcagaacag tttttaggca
    10741 agacaagaga agaactggaa aatgaagcaa gagaagtatt agaaggacac ttgcgctcga
    10801 ttcttggttc tatgactgta gaagaaatct atcaaaatcg cgataagttc agtcaaagcg
    10861 tacaagaagt cgcttctgta gaccttgcaa aaatggggtt gatcatcgtt tctttcacta
    10921 tcaaagaggt cagagacaaa aacggttatc ttgactcttt agggaaacca agaatcgccc
    10981 aagtcaaaag agatgcagat atagcagaag cggaagctct aaaagaaacg cgtatcaaaa
    11041 aagctcaagc agaaaaagaa tcacaagcag ctgaattaca gcgacaaaca gagatcgcag
    11101 aatcattaaa agaaaaagaa ctgaaattag caacctacaa acaagaacaa gatgttgcta
    11161 aagcaaaagc agaccaagcc tacaatttag aaagtgcccg agcacaacaa caagtaatcg
    11221 aacaggaaat gcagattaaa atcgtagaac gccaaaaaca aatcgaactg gaagaaaaag
    11281 aaatcacacg tcgtgaaaag caatatgatt cggaagtgaa gaaaaaagca gatgccgatc
    11341 ggtacgctaa agaacaagaa gcacaagctc aaaaagtcaa agaagtgact gaagccgaag
    11401 cagaacgatt ccgtgtggaa gcactggcag aagcggaagc caataagaca cgtttagctg
    11461 gacaagcaga agccgaagca gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa
    11521 agatcgccaa tgccttcaag gaatatggcg aagcagctgt cttgagtatg gttatcgata
    11581 tgctaccgca attgatgcgc gaagcagcac agccgctagg aaatatcgaa aaaatctctg
    11641 ttgtcgatac aggaagcagt tcaggagaaa ccagtggagc gaaccgagtg acaaattatg
    11701 ccacaaattt gctttcttct actcaagaaa cactaaaagc gacaacagga cttgacttga
    11761 aggaaatcgt agagaatttg tcgacacgtg ggactactag ccaagtaaat atgtatgaag
    11821 cgccaaaaga ggcagaacaa aactcgatga aggaagaata aagtagtaca cttcaccgtt
    11881 caatcaaaaa tggaggaaga ctttttaaag ttcttctctc cattttttta ttttattgca
    11941 aaagaaaatt agtcctcgct atttttcttt tgtttttgga tcttagcttc tatctcttgt
    12001 aaatttgttc ttagcaatcg ttctttcttt tggagttcga ccaaatcttc tgatagttct
    12061 tccttctttt ctgtttcagg aggacgaaga aagaaattga taccgattgc aatgaatgtc
    12121 cctataaaag tatctagtac acgttggata gcatacaaag cggattcgcc ttgagggatg
    12181 ctcaacgcaa tcagcaacaa cgtagctatt gcggaaatga taccggaatt attgttaata
    12241 ccatctgaca cgacgatcac aataataact agaataggaa gcaagatcaa ttctaccaaa
    12301 aaatcattgt gaaaatagct tttgacaaaa aaataaatga tagctaatgt accacctaat
    12361 gtattgccga tgatcctaga ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa
    12421 gaaaaaacag cagataatgc agcaatcatc gggattcctc gttgagtcaa atgaaaaaac
    12481 aagatacata aaaaaacagc taaggcactt ttgatcgtac gcatacctag tcgaaaacgg
    12541 ccaaattgca tagagattct cctttttggt ctttttacta tcatacctaa acttttgtct
    12601 gatagctagt ttttcattat tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt
    12661 ataggaatgc gaacagtcaa aacagtgatc agtgtcttct tttcgatggt cacagcgggt
    12721 agcttatcgc tgttgtattg gccagctgca gggatcattg cattattaag tgtaggaaat
    12781 accaagcgct ctacattgcg tacgggcatg aatcgaatcg cagcctttat attagctacg
    12841 gcaacagcac tgttcagttt ttttgctgtt gggtatacta tttttggctt cagtcttttt
    12901 ttattcctgt tcatcccgct tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat
    12961 tcggttttgg tcactcatta tttagtggag gaaaacatgt cttggcagct gatcggcaat
    13021 gaagcgggat taatggcgat tggattagta tttgctttgc ttgcaaatgt ttacatgcca
    13081 gatcgtacga agcagctgaa ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt
    13141 ttgagacaaa tggcagaatt tcttttatca gaaaataaag aagatgtgca gatcaaatgc
    13201 gaacatttac tgacattcat tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac
    13261 tactggctca gacagccgct atactacgaa acttacttct ctatgagaag agcacaggcc
    13321 aatgtgatta aagacatgct ggaaaatcta gagagaatcc agcaaccagc ttattatgga
    13381 aaacatatct atggtttatt gatttatact gcagaaacat tttcagagtc aaatgatggc
    13441 agacaaattt taacacgtat tgaagaagtt tatgtattgt atcggcaaat gcctctgccg
    13501 actagtcgcc ctgaattcga ggatcgagca gaattgttcc agtttttgca gtcttttaaa
    13561 agttttattg aaatcaaggc agaattctcc caacaaaaaa tccaaaaata gaggttgtga
    13621 attcagctaa tattcaggtc aaaaagctga tttcacaacc tcttctttaa acggtgttct
    13681 cctgtcaact cctcggtcac aagtcacaat attcaacaaa catgagaagc tgttttttga
    13741 aattgctcct tgtgactcgg agctgaacgg taggctcaca acctctttct taaacggtgt
    13801 tcaaaagctt tcctctgcgg taataagtcc agccaaacga aaaataggag gagctatttt
    13861 tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaaccttt taaaattaca
    13921 tatttttcca tagaacttgc atttgatcca agatttcgat gacatctttt gtcaagaaga
    13981 gagagcgatt gggcaagtca ccttcaatca tgtgtaccat attctttatt tcgtaattca
    14041 ttgcttcaga tgtattcccg ctttcgatga attcctttgt accatctgta aataagatct
    14101 ccgcacgatc agcacgcggg taatcagcaa tcgtcacata accgttctca aaggcaacga
    14161 taccgacttt tggcattttt gcctgaaaag tcaagctgac agcagcaagt tcattttctt
    14221 tgttacgcaa gatagtgacg gattgttcgt ccacgcctgt ttcaaaagga accattgtag
    14281 aggcaactac ttcaggttga gagctcaaaa agaaacgagc gaaagataca gcatacgtac
    14341 cgatatccaa cagagctcct ccagctaatt ctggattgaa gaaccggttt ttagggtctg
    14401 gttctttgta actgccgaaa ggtgcttgga tcatttttaa agcccccagt tttcctgtat
    14461 ccattattga gcgtaactgc tgataaagtg gcatgttgaa gattgtcatc gcttctgcta
    14521 agatcaaatt cttttcttct gctaagcgca tagcgtcagc cagttctttt ttattcatcg
    14581 tgatggcttt ttcacataga acgtgtttgc ctgcttccaa tgctgccaag atatggtcga
    14641 tatgctgtct atttggtaca gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt
    14701 cttgatagct tccgtaagct tttggaatat tgtaacggtg agcgaagttt tccgcttttt
    14761 ctgaagttct ggaagcaact gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa
    14821 actcatgtgc gatggtacca agaccaacga ttccccattg atattttttc atctgaaaac
    14881 gctccttcat agttggattg agtttcttgt ctgtctatta ttataacaaa aatcacgaag
    14941 aataaagagg agaggacttg aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct
    15001 ttttgatcaa aacttaagta gaatagttaa aaggagggga taaacgtgca tcaagtatat
    15061 attgatctta ttatgagtgt attattagaa cagtttgaaa ctgaaaaaaa ttttttagat
    15121 gaatacttgc tgatcagtcc agaacaatgg caaaaatgga aagatggcga gaaaaatctg
    15181 ccatctgaag cgatgcagaa agtaaaaaat cttttcactg attatgaatg gatgctaacg
    15241 caaaagattt tgcgtcaaac gattattttt cccgaaaaac gaaacgttgc cgttgcagag
    15301 tacaagcaaa tcaaaacacg catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc
    15361 gagctgatcc ctttgaaaaa taatcagcag gctggtagat accttgactt gaaagtaagt
    15421 attcaatatg atgaatgggg ctatgatgat attttgaatt ttcgtctgcc agcagatgtt
    15481 caaaaacaga tcgaaggaga aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa
    15541 gaaacttacg ttcatgaggc gaatggggaa cgtgaagaac aacgaaagga tttttcaagt
    15601 gaataaattt ttaggatttg gagcagttgt gattatgatc gcagcaatgg gctatgttat
    15661 ttttagcgat gatgattcgc atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac
    15721 caaaacaact gctagtaaag aaaagaacag tcaaacaacc agcagcaagg taaaggacaa
    15781 agaaacaaaa gatcgtacgt ccaaacaatt gccagatgtt aaaacagacg attggaagct
    15841 agttttggtt ggtccagatc acaaattaga aaaagaaatt gatgagtcta ctcaattggc
    15901 atcattaagt aacggatact tgatcgacaa acgcattgct ccagaatatg aagcatttgc
    15961 caaggctgct gaagcagctg gatttccatt agtgatggtc tcagcttacc ggtctgtatc
    16021 atcccaacaa caagttttcg aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc
    16081 agaagaggag gcgacaaaga ttactaaaga aacgattacg gagccaggat acagcgaaca
    16141 ccacacaggt ctggcagtcg atgtcgtcga tcaaaactgg tacaacagtt atccttctac
    16201 tgtattggat gcaagttacg gagatcagcc tggagctaaa tggatcgcag ataatgctgc
    16261 gcagtatgga tttatcgttc gttatccaaa aggacacgaa gatataacta agatcacata
    16321 tgaaccttgg cacttacgtt atgtcggtaa agaaaacgct gagtatatca ccaaacacca
    16381 attgacgcta gaagaatatc tgaatgaatt aaatgaaaag taggaattga tcatggcgaa
    16441 aaaaaagtgg aaaaagcaaa agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt
    16501 agcagtgata ctcttagcct ttgttttttc cttgaagaat ctgtcttctc cttttactga
    16561 taatcagcaa gaaaatgaag cgcaatccca tcagcaattt attgaacggc ttgttccgca
    16621 tgcccaagaa ctacaagatg gttatggagt gcttcctagt atcattttag gacaagctat
    16681 attagaatca aattggggaa aaagtacctt ggctagcaaa tacaataatc ttttcgggat
    16741 caaggcttat ggggatcaga agaaagtaag cctggaaaca aaggaatttg ttaacgaaga
    16801 atggataact atccaaggag acttcaaagt gtatgattca tgggaacagt ccatggatga
    16861 ccatacacaa ctgttcgtcc aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat
    16921 tacggccacc aactatcagg aagccgcaca agcattgcaa gatgcaggtt atgcgacaga
    16981 ccccggttat gcgcaaaaaa tcatccaggt catcgagacg tatcaattga atcagtatga
    17041 ccggagatag caaaggagaa gtggctgatg gcagaacaat ttacgccaga aatcatgacg
    17101 gaattgagaa aagatatcgt aagagtaccg gaaatcattt tagaagcaag cgggatcaag
    17161 atatttggaa aattgatccg ttcgatcatc tttactacgg atattgcgat catacgtaat
    17221 acaaatgcga atgcagtaat tgctgtgtat ccttttacac ctcatccagc gattactaaa
    17281 agcattattg aggcagctga cattccagtt ttctccggtg taggtggtgg tttgacacaa
    17341 ggtgctcgtt cttcctacat gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt
    17401 ttgaacggtc ctacgccttt agagacagta aaagaagttt gtcgagtagt tgatattcca
    17461 gttatcagta cggtaacgtc gaaatataca gaaatagatg aaaagttagc agctggtatt
    17521 tcgattatca atatcagtgc aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa
    17581 aaatatcctg aacttcctat cattgcgaca ggtggtccaa cggaagaaag tattgctgag
    17641 accattcaag caggagcaaa tgccatcaca tatacgccac caagtagcgg agagcttttt
    17701 agtcgtaaaa tggaaaaata tcgggaaact gagaaaaaga atcatgaata acaggtgaga
    17761 atcaaaagac gaggaagagc agatagtggc tcttcctcgt cttttgtact ccagtactat
    17821 ttgttcagaa ttcaaaaaaa agagaaattt ttttgttttt ttacctgtaa tgtacataag
    17881 aagagttaaa accttttgtt attattagca ttctaacaaa aaaatgtctt gatttattga
    17941 agcgctttct atagaatgaa aaacgaataa aaactaaaaa aggcacccga caatttagta
    18001 gcagctaaat tgtcggggct tgaaaagtca gcgggaactg accaatcaag ttgccatagt
    18061 caatgcttgt gcatcgacgt gtatattgta cctaatttca tacagaatta ctagttattt
    18121 ttctgttttt tagggaagag cgcacacgaa gaagcatgct tatttagcta tgtcaaggat
    18181 attgggcagt tgtctgatat ccttttttct ttgcgtaatt atcctcccat cattgaagca
    18241 agaagtgttt ttagcgatga aagaagaatc agcactatct aatttttctt gaaagtattg
    18301 agttgttcat cctaggcaga acgataaggc aagtcttggt ttttattccc cttgaaaaag
    18361 atcgtttaat aaatatgttc tgtataaaaa tacagtatat tgaaaaataa caatggaagt
    18421 agaggtgcag agaatgaaga aaagaatgag aatcatctta gctttaagtg ttatgttatt
    18481 cttcttgacg gcttgtgggc aagaagatga caaaaaaatc acgctgaaca tggcttggtg
    18541 gggttcgcaa gtccgacacg atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa
    18601 tccccatgtg acgatcgatt atgaatttta tgattatgaa ggatattaca caaaattagg
    18661 tacactagca gcatcagatg atatctggga cattttccaa ctgaacaatc aatttcctca
    18721 atacatcaat cagattgaac cattagatga atatgtggag caagggataa ttgatatttc
    18781 taatgcagat gaatcctatt tagcaacgac gacttacgac gggcaattag ttggactgtc
    18841 taacggggtg aattcgtttg cgattgctta caataaaaat atatttgaac aattaaatat
    18901 ccctgaacca gcttcaaatt ggacatggga tgaatttgaa caaatcagct atcaaatcac
    18961 tgaagaatta ggaatctatg ctatttcaag attcgaagat tttatagctg gctgtattat
    19021 tcaaatacca caagttgaga aaggattgaa tttttataat cgggaagatc tatctaacag
    19081 cttgggattt gaaaatccag actatctaac agattttttc cagctgcgta aaaatctggt
    19141 agacaaaggg gcccatccag aaccaggtgg tggagctgca agtacgacgg atctgtcttt
    19201 tcaggcggtt agggatagag aagcggcaat ggtgtttcta tcaagtaacc agttgacaga
    19261 aatattgtca ggggctcctg aagatatgga gattcagttg attaatccac ctcgcagaaa
    19321 agcagatgga gaatctggta tccctttacg ttcttcgcaa atgctgagta tggcaaaaag
    19381 ctcagcccat aaagaagaag ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc
    19441 caatgaaatt ttaaaaggag aacgtggtgt accaatcatg tcgaatattc gagaacagct
    19501 cgctcgtgca gatgatgcaa ctttgcaagc gacatttgat tatttagaaa tgatttccga
    19561 tatggacaac ggcgaagtga atgtctttga tagtgctttg aatccggaaa ttcaagacca
    19621 atacaatctt ttagttgaaa aagtcattta tgaagaagta acagcagaag gaaggcatga
    19681 aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac gtatttgctt
    19741 ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg tcactttatt
    19801 attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg gataattata
    19861 taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt ctctatgtat
    19921 ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt ttaacgagga
    19981 atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg atcggtggca
    20041 gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta gtgaatattt
    20101 tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa ctggtcatga
    20161 tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc tttgctgcag
    20221 gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga gcgaacaaat
    20281 ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg tataacttgg
    20341 ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt acccaaggtg
    20401 gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct ttttctttta
    20461 attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg ggagctgtca
    20521 cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga ggaggaagaa
    20581 atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt aggtctagtc
    20641 atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca agagattatg
    20701 agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc aaaaggatgg
    20761 gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt catctctgca
    20821 gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt atcgagaatc
    20881 gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat gattcctgca
    20941 caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt tgggacgtat
    21001 gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca gatcatgcaa
    21061 ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg atgcagcaag
    21121 tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat cacgacgatc
    21181 atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta tttaaacaag
    21241 ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg atctagtacg
    21301 acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt tctgattttc
    21361 ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa gggctgaata
    21421 tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc tgtcggattt
    21481 ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt ttgttcactt
    21541 taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg atgtatcctt
    21601 cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg aaagggaaaa
    21661 tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt tttgcagttg
    21721 attggtattt attagtggga aagattggag cttggtatct gattcctttt ctattgtgtc
    21781 tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc aaagaagata
    21841 aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca aaaaacgtca
    21901 aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata cagcctgatt
    21961 ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt tgcttaaaag
    22021 aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg gtgggagagc
    22081 gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac tagtcggttt
    22141 ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga acaagtatgg
    22201 acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg atgacgtacg
    22261 gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata tcattcgtgc
    22321 aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg aggaaaaagc
    22381 tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca cttttaatta
    22441 tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg tgattggtga
    22501 tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag attatttcag
    22561 aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat ctacccatca
    22621 ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg cattcggtgg
    22681 attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat tttacagtcg
    22741 atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt cagccaatga
    22801 ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc gtgaccgcag
    22861 tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca aatatgaaac
    22921 tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat tcaatgtttc
    22981 tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt acatcaatgc
    23041 aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg tctatccgat
    23101 gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg gcggaggaga
    23161 tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt ggaatcgggc
    23221 agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa atcattctat
    23281 tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat aagaatagat
    23341 ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata ggctgtcttt
    23401 tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc cttcttttta
    23461 tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc gaacaaaaag
    23521 gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac gttgtcagtt
    23581 tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat tcaaatatgg
    23641 caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat tattagtttt
    23701 acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt accgtaaatg
    23761 ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata aagtggaggg
    23821 tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt tcggtattaa
    23881 taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa aaagacggac
    23941 gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa gtagaccctg
    24001 tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa ggaatcacga
    24061 aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca agagaattag
    24121 atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa gaattattga
    24181 cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt tctcgtaaat
    24241 ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac ggacaagcgg
    24301 caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga atcgggatcg
    24361 tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga ttgagagtcg
    24421 tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt gaggaggacg
    24481 cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc tgcagcatct
    24541 gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag gactaggctt
    24601 taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg gattagcgac
    24661 attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg ttttaggatg
    24721 tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag taggagctat
    24781 ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg tcttttctaa
    24841 aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta ttggtttgac
    24901 tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag ctgattttgg
    24961 tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag gcgtgcaaat
    25021 gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg gaggaagcat
    25081 cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc cgttgttcca
    25141 tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga ttttactaat
    25201 gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg cacttggtga
    25261 cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg cagaaggact
    25321 tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt ctcaaaatgt
    25381 cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct ctgcattctt
    25441 cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca tccctgagcc
    25501 ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag gaatccgtat
    25561 gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg tttctgttgg
    25621 atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc aaacattgca
    25681 aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat tgaatttgat
    25741 ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga agtaatagag
    25801 caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca aacggaaaaa
    25861 tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg attgaacacc
    25921 gtttatccat aaaaaggaat gtgacctaac ttttgtcaca ttcctttttt tcatctttaa
    25981 taaacacgaa caataaaatg tttttctttg ttaacattcg aaatttcttt gacatcaaaa
    26041 aaagtgattg ttaaaatatg aatgtagaaa aactgggagg aaaaacaatg acagcaaaaa
    26101 tctctgtgat tatggggagt acttcagatt gggaaacaat gaaagaagca tgtcaaatat
    26161 tggatgaatt cggtgtgtct tatgaaaaaa aggtcgtttc tgctcatcga accccagatt
    26221 tgatgtttga atatgcacaa aacgctcgaa aaaaaggaat caaaatcatt attgcaggag
    26281 cagggggagc cgcgcatctg ccgggtatgg tcgcatcaaa aacaactttg ccggttatcg
    26341 gtgtaccggt ccaatcacga acattaaatg gtttggattc gcttctttcg attgtccaaa
    26401 tgcctggagg agtacctgta gctactacag caatcggaaa agcgggagca attaatgctg
    26461 gactacttgc gatacagatg ctttccatgt atgatttaga attggaggct aaattggctg
    26521 aaagacgttc aatgttggct aaaactgtta tagaaagtag tgatcaactt ggttaaacct
    26581 ttgttgcctg gttcaacgat cgggatcgtc ggtggcggtc aattaggtcg tatgatggcg
    26641 attagtgcaa aagaaatggg ctttaaagta ggtgtgctgg atcctgtgag cgattgtcca
    26701 gccgcacaag ttgcagattg gcacattgaa agtgtctatg atgacacctt tgcgctgaaa
    26761 gagcttgcca gaagaacaga tgtcattacc tatgaattcg aaaacgtcag tgtagaggcg
    26821 ttaaatgcga ttttacccat gtcatttatt cctcaaggga cagacttgct tgcgattacg
    26881 caagaccgcc tgttagaaaa atcttttttg gaaacgaata acatcgtgat tgctccttat
    26941 gcaacgatcg tcagtccgac ggatatccaa gatgcgattg acgggatcgg ctatccttgt
    27001 gttttgaaaa ccactcgagg cggctatgat ggcaaggggc agtatgtttt gaaaagtcgg
    27061 gcggatctag ctcctgccat ggacctttta agagaaggaa cctgtgtttt agaagcatgg
    27121 atcccgtttg aaaaagagat ttcgatcatg gtggcaggaa atggtcaagg agatttcacg
    27181 acttttccgg tcgtggaaaa catccaccac aacaatattc ttcatgaaac gattgctcca
    27241 gccgcgattg atcaggatgt gatcgaggag gctgagcgta ttgcccgtgt gattgcagaa
    27301 gcagtgagcc tttcaggtgt tttaggggta gaaatgtttt taacaaaaac aggcgggcta
    27361 tatgtgaatg aattagctcc tcgtcctcac aattctggac attattctat tgaagcttgt
    27421 tctatgagtc agttcgatgc ccatattcga ggtatttgtg gttggccatt aggagaaaaa
    27481 gtacgtttga taagcaatgc agtaatggtc aatgttttgg gagaacaact gctggaaagc
    27541 tatcgcttga ttgagaataa gccagactgg caattccatt tctatggaaa aaaacaagca
    27601 aaaaaaggtc gcaagatggg acatatcact atcttgacag aagacatcca gcaaacgctg
    27661 gaagaaatca aacaaacgaa tatttgggat tagaggagaa aaaacatgtt agaacgttat
    27721 acacgccctg aaatggggaa tatctggacg gaagagaaca aataccgtgc gtggctcgaa
    27781 gtcgagattt tagcagatga agcttgggca gaacttggtg aaatcccgca agaagatgtg
    27841 aaaaaaattc gtgagcatgc atcatttgat atcgatcgga tcttggaaat cgaaaaatta
    27901 acacgccatg atgtcgttgc gtttacacga gcggtttctg aaacattggg agaagaaaga
    27961 aaatgggtcc attatggtct aacaagtacg gatgtcgtag atactgctta tggctatctc
    28021 atcaagcaag caaatgatat tttacgggaa gacttgaaac gttttacgga aatcattggc
    28081 gaaaaagcaa aagaacacaa atatacagtg atgatgggtc ggacacacgg tgtccatgct
    28141 gaaccaacta cttttggatt gaaacttgcc acatggtatt cagaaatgaa acggaacatc
    28201 gaacgatttg aacatgcggc aaaaggagta gaagcaggaa aaatctcagg agcagtggga
    28261 acgttcgcaa atatcccgcc atttattgaa gagtatgtat gtaaacaatt ggggatccgt
    28321 ccacaagaaa tctctactca agttctccct cgggatctac atgctgaata tttctcagcg
    28381 atggcgctta ttgctacaag tattgaacgt tttgcaacag agatacgcgg attgcaaaaa
    28441 tccgaaacac gtgaagtaga agaatttttt gcgaaaggtc aaaaaggttc ttctgctatg
    28501 ccgcacaaaa ggaatccgat cggttcagaa aatatggctg gtctagccag agtggtgcgc
    28561 ggacacatgg tcactgccta tgaaaatgtc acattatggc atgaaagaga tatttctcat
    28621 tcttcagcag aacgaatcat cttgcctgat accacgattt tattgaatta catgttaaat
    28681 cgttttggca atatcgttaa aaatctaacg gtctttcctg aaaacatgaa gcggaatatg
    28741 tctgcaacat ttggtttgat ttatagccag cgcgttatgc tgaaattgat cgataaggga
    28801 atgacaagag aagaagctta tgatcttgtt cagccaaaaa ctgcgtacgc ttgggacaac
    28861 caagtgcagt tccgcccgct gttagaagaa gatccaacca tcacatcggt attatctaaa
    28921 gaagaaatag acgatgcctt tgattatcat catcatttga aaaacgttga cgtgatattc
    28981 aaacgagtag gtttggaata aagaagagtg agttgcaaaa ttcaattaaa atagaatagc
    29041 aaaaataacc aaaaaagaag cagatccgtt agcggttctg cttcttttag tttattatat
    29101 tttcttatca ataataggaa cctgatttta aaagcggcta cctctttacc gtgcctcttt
    29161 acatacaaaa aatagaggaa ttaagcgtaa atggcttggc tattataact aaaatgctgg
    29221 ttatggagat gttgcagcaa gttttcgaga aaagcgtatt ccatctcttg tccattgaat
    29281 aagatctgga tacgataatg atttttgaaa cgcttctgtt catgaatctg actgattgaa
    29341 agttcgcctt tgtgttctgt ctgataaaga aaagtccttt tacggcattc agcacagcgc
    29401 ttggttgctc ggcggatttt tcggaatgta taaaaactag tatctttatt gcataaataa
    29461 acgtctttga tcgacagcaa atcattatat agacaggaac gttcttcagc tgttaattct
    29521 gataaaaaaa gctgaccttg ttttattgca tcttccatcg gcaagtgcct cctcgaaccc
    29581 attatccaaa tttttagtta attctaatat accatcatta agtataaatg taaacgcttg
    29641 ttgatggtat aagattttcg tttctgataa aacacgaaca ttaaaagaat ctaatgcctt
    29701 aaatgtacat atttttgttg atttataaaa agatgattgc taaaatttaa agtgaaaaaa
    29761 cgaacaaaaa acgatgtatt tgcttttaat gtaaataaaa aggagagacg tacatggaga
    29821 agaaagaact tttgtatgaa ggaaaagcca aaaaaatata tcttacttct gatgagaact
    29881 tgatttggat agaatattta gatcaagcaa ctgcgttgaa tggcgcgagg aaagaccaga
    29941 taagtggaaa aggagaattg aataatcaaa tcacaagtgc aattttccgt tatctgactc
    30001 aaagaggaat aaaaaatcat tttattgaac aattgtctaa aaccgaacaa ttagcaaaaa
    30061 aagtacaaat tatccctctg gaagtggtta tccgaaatat tgcagcagga agtttctcaa
    30121 aacgtctagg tgttcccgaa ggtaaagaat tcatcaaacc aatcattgaa ttctactata
    30181 aggatgacca gttggatgat ccatttatca atgaagagca tatctatttc ttacaacttg
    30241 caaaaccgga agaaatcagt gagctgaaag cccaagcgtt ggctattaat gaaattttga
    30301 ccgaattatt tgctaaggtc ggtttggttt tagtagattt caagctagaa tttggccggt
    30361 cgaatggaca aattttgctt gcggatgaag taacaccgga tacttgccga ttatgggatg
    30421 aacgtacgaa tgaacatatg gacaaagacg tttaccgcag agatctaggt gatttgatcc
    30481 cagtctatca agaagtatat aaccgtctat cggaactaac tgaagaaaag gagcgagtat
    30541 gatgtatttc gtaaaagtat atgtaaccta taaagattca gtgttagatc ctcaagggga
    30601 ggcagtaaaa aaagcagtcc atcgtatggg atatgaaaca atcgaagata tccgaatagg
    30661 aaaatacttt gagatacaac ttgctgatgc agaagaacca atcgatacag tgattgaaac
    30721 gatttgtgac aagctgcttg caaatgtaaa tatggaaaca taccgatatg aaatcaaaaa
    30781 aatggaggaa gtataacatg aaatttgcgg tcatcgtttt tccaggttca aattgtgacg
    30841 cagatatgtt atgggcagtt cgcgagatca tgggcgctga tgccgaattt gtacggcatg
    30901 atgagtcctc tttagaagga tttgatgggg ttttactgcc gggaggattc tcctatggtg
    30961 actatttgag atgcggtgcg attgctcggt tttctacaat catggaagaa gtgattcgtt
    31021 ttgctgaaga agggaaacca gtattcggta catgcaatgg attccagata ttgacagaag
    31081 caggactttt gccaggtgta ctgagacgaa atgagtcttt gcattttatt tgcaaaactg
    31141 taccattgaa agtcaacccc aagacggcct ttacttcaga gtatgaagcc gacgaaataa
    31201 tctatcttcc agttgctcat ggggaaggaa attatttctg tgacgaagaa acacttcaag
    31261 aactaaaaaa aaataatcag attgccttta cttataatga caacatcaac ggcagcgtgg
    31321 aaaacattgc agggattacg aacaaagcag ggaatgtgtt aggaatgatg cctcatccag
    31381 aacgtgcagt agaagacttg ctaggatcag aagatgggaa acggttcttt gcttccattt
    31441 taaaaaactt cggaaaggtg accgtataga tgaaaacatt gaaagaaccg acagctgaag
    31501 agatcaagaa aaaaagaatc tatgccgaat ggggattgac tgatgaagaa tatcgaatga
    31561 tcgaagaaga tatcttagga agaatgccta attatacgga aactggactg ttttctgtta
    31621 tgtggagcga acattgttcc tataaaaatt ctaaaccggt tctacgcaaa tttcctacta
    31681 gtggacctca tgtgttgcaa ggccctggag aaggcgcagg catcgtcgac atcggtgatg
    31741 gactagcagt tgtcttcaaa gcagaaagtc acaatcatcc ttcagccgtg gagccttatg
    31801 aaggagctgc gacaggagtt ggcggaatca tcagagatat tttcagcatg ggggctcgtc
    31861 caattgcgtt gctagattca ttgaggttcg gcgaattaga taatccaaga acgaaatatt
    31921 tactagaaga agtagttgca ggtatcggcg gatacggcaa ttgtatcggc ataccgactg
    31981 taggcggcga aattgcattt gatccttgtt atgaaggaaa cccattagtc aatgcaatgt
    32041 gcgtaggttt gattgaacac aaacatatcc agaaagggca agccgctggc gtcggtaatt
    32101 cgatcatgta tgtaggtgca aaaacaggtc gcgacggtat ccacggagca acttttgcct
    32161 cagaagaatt taatcaagaa gaagaacagc aacgttctgc tgtacaagta ggcgatcctt
    32221 ttatggaaaa gctgctgctt gaagcgtgtt tggaattaat cttgcatcat tcggattctc
    32281 taataggtat acaggatatg ggagcagcag gacttgtttc atctagtgcg gaaatggctt
    32341 caaaagctgg ctcaggactt atattgacat tagatgaagt cccacaaaga gaaactggga
    32401 tgacacctta tgaaatgatg ttatcggaat cacaagaacg gatgttgatt tgtgttaaat
    32461 caggagagga agaaaaaatc caagaactat ttcagaagta tgatctagat gcagtcacta
    32521 tcggtaaagt aacagacgac gggcaatatc ggctatatca tcaaggagaa gaagtagcaa
    32581 atctgccagt cgatgcatta gctgaagatg caccagttta tcataaagaa atgaaagaac
    32641 cagctcgaat cgtagaattt cagcagttag ctccttatca gccagttatc gaagaacctg
    32701 gacaagtatt gcttgatctg ctgcagcaac cgacgattgc ttcaaaacgt tcaatctacg
    32761 aaacttatga ttcacaagta caaaccaata cagttgtccg tccaggaagc gatgctgctg
    32821 tgatgagggt tagagggacg aataaagcgt tagctatgac cacagactgc aacgcgcgct
    32881 atctgtattt ggatccaaaa atcggaggac aaatagcagt ggcagaagct gctagaaata
    32941 ttattgctag cggtggtaag cccttggcga tcaccgactg cctgaactat ggttctcctg
    33001 ataaaccaga agttttttgg gaattatcga cttcagctga cggaattgct gctgcgtgtg
    33061 aaacgttagg aactcctgtt atttcaggaa atgtctcttt atataatgaa acggatggtc
    33121 aagcaatcta tccaacacca atgattggta tggtcggatt gattgaggat cataaacata
    33181 tcactactca ggaattcaaa aaatcagggg atctaatcta tatattaggt aagacgtttg
    33241 cagattttga tggcagtgaa ttacaaaaga tgcagcttgg aagaatcgaa ggtgtgatcc
    33301 gaaactttga tctatccatt gaaaagagaa accaagagtt agtattgaca gccatccaaa
    33361 acggattgat tgaaagtgct catgactgtt cagaaggtgg cttggcaatt gcactggcag
    33421 aatcagcttt caaacatcag ctagggatat ctgtacagtt tgaattgtcc tctgctcaat
    33481 tatttgcaga aactcaatca agatttgtgt tgacagtcgc accggaaaat aagacacgat
    33541 tcgaagaaat gatgggagat gctgctgttt tagcaggtaa ggtgacggat gaagcgataa
    33601 tcgaaatttc agcaactgac ggtcagatca aaatagaaac tgcagtagca agaaagtgct
    33661 gggaggatgc aatcgtatgt cttatgaagt gaagagttta aatgaagaat gcgggatctt
    33721 cggtgtttgg ggtcatccag acgccgctag tgtggcatac ttcgggctcc atagtcttca
    33781 gcatcgagga caagaaggtg caggtatcgt gtccaatgat cgaggaaagc ttaaggggca
    33841 tcgagatttg gggctgcttt ctgaggtatt caaagatcaa agaaaattgc agcagttaac
    33901 aggagaagct gcaatcggac atgtacgtta tgcaacagca ggcaatggaa gtgtggacaa
    33961 tattcagcct tttctattca aatttcatga tcagtcgatc ggcttagcgc ataatggtaa
    34021 cttgatcaat gctcgttccc ttagaaaaga gttagaggaa aatggggcga tcttccattc
    34081 taactccgat acggagatat tgatgcattt gatccgacga agtgaagaaa cagatttttt
    34141 aaataaatta aaaagtgctt tgaatgaagt caaaggtggc tttgcctatt tattaatgac
    34201 agaaacggca atgattgccg ctttggaccc aaatggtttt cgtccattag caatcggaca
    34261 aatgaaaaac ggagcatacg tgattgcctc ggaaacttgt gcattagaag ttattggggc
    34321 acgttttata agagatgtcc agcctggtga gatagtgatc atcaatgacg aaggaatcca
    34381 gattgattgc tttacgaagg acacgcagct ttctatttgt tcgatggaat atatctattt
    34441 tgccagacca gattcaaaca tcgcagggat caatgtgcat actgcacgga aaaatatggg
    34501 acggaattta gcgaaagaat cgccagtaaa agcagatatg gtcgttggcg tacctaattc
    34561 ttctttgtca gccgcaagcg gttatgcgga agctagtgga atcccttatg aaatcggatt
    34621 agtaaaaaat caatacattg cccgaacatt tatccagcca acgcctgagc taagagagca
    34681 aggtgttcga atgaaattat ctgctgtacg aggagtagtg gaaggtaaaa aggtgattat
    34741 ggtcgatgat tcaattgttc ggggtacgac aagcagacgg atcgtccatc ttttgaaaga
    34801 agcaggggca aaagaagtcc atgtgaggat tgcttctcct cctttgaaat atccatgttt
    34861 ttacgggatt gatattcaaa cgcgtaaaga actcattgcg gcaaatcaca cgattgatga
    34921 aatcaaagaa tgtatcggtg cagattcttt aggtttttta agtgaagagg gtctgatcga
    34981 atcgatcggt ctggaaaaag atgctccgta ttcaggattg tgtatggctt atttcaacgg
    35041 agattatccg acgccgttat atgactatga agaaaaatat caggaatcac taaaagaaaa
    35101 agtatcgttc ttttaaaagc agaccaagaa gggagtatat gaattcaatg gcaaatgcat
    35161 acgcaaaagc tggcgtagat gtcgaagctg gatatgaagt agtagaacgg atcaagaagc
    35221 atgtcaaaaa aacggaacgt ttaggtatga tgggcgctat aggaggattc ggcggttgtt
    35281 ttgatttatc ggtctttcaa ttaaaagagc cagtccttgt ctcggggacc gatggcgtgg
    35341 ggacgaaatt agcagtggca atcgaagcag acaaacacga tacaatcggg atcgactgcg
    35401 tagcgatgtg tgtcaatgat atcttagcac aaggcgcaga gccgttatat ttcttagact
    35461 acttagcaac ggggaaaaat gatccgttac gtttggaaaa tgtagtaaaa ggtgtagcag
    35521 atggatgtgt tcaaggaaat atggcgctga ttggcggaga aacagctgaa atgcctggta
    35581 tgtactcagg taatgattat gatctagccg gatttgctgt agggatagct gaaaaatctc
    35641 aattattgac aggagaaacg atcaacccag gagatgtgtt gcttggaata gcatctagcg
    35701 ggattcattc aaatgggtac tctcttgttc gcaaaatttt ctttgagcag cagcagtttt
    35761 ctttatcatc aaaactgcca gaattgaaaa aaacacttgg cgaagaattg cttgagccaa
    35821 cgaaaatcta tgtttcttcc ttgcttccgc tgatcaaaga aaagaaaata aaaggagctg
    35881 cacatatcac tggtggaggg tttgtagaaa atatcccgcg aatgttacca gaatctatgg
    35941 ctgcacaaat caaacttggc tcatggccga ttcttccaat attcggctgt ttacaaaaat
    36001 atggtgaaat tcctgagatg gagatgtatg aaatcttcaa tatgggcatc ggtatggtcc
    36061 tggcagtcga agaatcgaaa gtaaaagaaa tagtacacga aattgagcaa gctggcgaaa
    36121 aagtttatga aatcgggaaa gtgatcgcca aagaaaaaca tcgtgtggtg tttataggag
    36181 aagaacgatg agaatcgctg tttttgcttc tggaaacgga agcaactttc aagcgctagc
    36241 cgattatcta tcgaaaaaag gattggaatc ttcgattgac tggttgtttt gtgatcagcc
    36301 tgaagcatat gttttgaaac gtgcaactgc gttgagtgta ccggcagatt gtttttcacc
    36361 taaagaattt gattcaaaaa aagagtatga agaagcaatt ttgcataaat tgaaagaaaa
    36421 aaagatcgat ttgatcgtat tagctggata tatgcgaatc attggaccgg tattgctgaa
    36481 aaattatgat aaaagaatca tcaatatcca tccgtctctt ttacctgcct tccctggttt
    36541 gcatggcata cgtgatgcct ttgaagctgg agtaaaagaa acgggtgtca caattcatta
    36601 tatcgaccaa ggtgtagata ctggaccaat catccggcag gaaaaagtga gaattgaaca
    36661 ggaggacacg ttcgattctt tagaagaaaa aatccataga gtagagcatc gaatctatcc
    36721 agaagtgatt agtgaaatta ttgaaaatgg aggctatctg aaatgacaag agcattgatc
    36781 agtgtatcag ataaaactgg gattgtagca ttcgcccaag gtttgattga acaaggagta
    36841 gaaatcattt caacaggagg aacaaagcat gcgttggata aagctgggat caaaacgctt
    36901 tcgatcgaag aagtgacagg gtttcctgaa atgatggatg gccgtgtcaa aacacttcat
    36961 cctaaaatcc atggtggtct tttaggaaga cgcgatctcc ctagccatat ggaggcaatg
    37021 gaaacggaaa atatccaacc aatcgacttt gtttgtgtca atttatatcc tttcaaagaa
    37081 acgattatga aaccagatgt cgaaatagca gaagctattg aaaacattga tatcggtggt
    37141 cctagtatgc tccgtagtgc agcaaaaaac catgaatttg tcacctctgt tgttgatcca
    37201 tctgactatg aagaagtact ttcagaatta cgtcagtatg ggcatacttc actttctttc
    37261 agacaaaaac tagcggcaaa agtctttcgt catactgcgg catatgatgc actgatcgct
    37321 caatacttaa cggattgggt ggaagaaaaa gaaccggaaa aattgacact tacgtatgaa
    37381 cgaaaacaaa cgcttcgtta cggtgaaaat agccatcaag ccgctgcctt ttatcagtcc
    37441 gctcttctgg aaaaatattc gatcgcagca gcaaaacaac tccatgggaa agaactttct
    37501 tataataata tccgagatgc agatgctgcg ctacggatca tgaaggaata tgacgagcca
    37561 acggtagtcg ccttgaaaca catgaatcct tgtggtattg ggacagcatc tacgatttta
    37621 gaagcgtggg aagcagcgta tgaagcagat cctgtttcaa tctttggtgg gatcatcgtt
    37681 ttgaatcgag aagtagatct gccaacagca gaacagatgc ataagctgtt tctagaaatc
    37741 atcattgccc cttcttatac acaagaagct ttggaattac tgcaaaaaaa gaaaaacatc
    37801 cgattactga cagtagactt tgcgcaggaa gaaaatcaaa atcgagaaga aacggtctca
    37861 gtcttaggtg gacttcttgt acaagatcaa gatctggcaa tagaaaatga agaagagtgg
    37921 aaagtcgtga caaaacgtaa gccgactcca gcagaagcaa aagcattagc gtttgcttgg
    37981 agagctgtca aacatgtgaa atctaatgcg atcgtactgg caaacgaacg tcagacagta
    38041 ggaatcggag ccgggcagat gaatcgagtc ggttcagtaa aaattgcgat tgaacaagcg
    38101 caagagaaaa tggaaggtgc agttttagct agcgatgctt attttccaat ggatgattcg
    38161 gttgaatatg cagccaaaca tggtataaaa gcaatcattc agccaggagg aagcatcaaa
    38221 gaccaagatt ctattgatat ggcgaataaa tacgatctag caatggtctt tactgatgtt
    38281 cggcatttta gacattagag aggagtcgga taaatgaaca ttttggtgat cggtgcaggt
    38341 ggtcgtgaac atgcaataag taaaaaactt ttagccagtc cgaaggtgga tacggtgtat
    38401 tgtgcgccag gaaatccggg gatgacaaaa gatggtatca attgtgtcgc tatcagtgag
    38461 cgagatcatt cagcgttaat ccattttgtt aagaaagagg ctattgattg gagtatcgtt
    38521 ggtcctgaag ttcctttgtt aaatgggatc gtcgatgatt ttatggcgga aggattgaaa
    38581 atcttcggtc cgacaaaagc agcagctctg atcgaaggtt cgaaaagttt tgctaaacaa
    38641 ttaatggata cttatcaaat cccgacagct aaatccagaa gttttgaatg ttacgaaaaa
    38701 gcagcagcgt atatcaaaga aaatggtgcg ccaatcgtag tgaaagcgga cggcttagca
    38761 gctgggaaag gtgtagtcgt ggctaccaca gtgaaggaag cgctggatgc ggcagaacaa
    38821 atgttgaacc agcatcgatt tggggatagt agcaaaaaaa tcgtcgtaga agagtttttg
    38881 gcaggagaag aattctccct tttggcgttt gtccgtaata ccaaggtgta tccgatggtc
    38941 atttcgcaag atcataagcg ggcatttgat aacgataaag ggccaaatac aggcggaatg
    39001 ggtgcttatt caccagtacc gcaaatttca aaaaagatga ttgaaaaagc tgtggaagaa
    39061 gttttaaaac cagcggcttt gggaatgaaa gaactaggac gctctttcac gggtgtttta
    39121 tatgcgggtt tgattgctac gaaggaagga ccaaaagtga tcgaattcaa tgcgcgtttc
    39181 ggtgatcctg aaacgcaagt cgttctttct cggttgaaga gtgactttgc gcaagtaatc
    39241 gatgatttat tagagaatcg tacagtagag ttaaagtggc agcaagaagg atacaatgta
    39301 ggcgttgtgg ttgcagctgc cggttacccc gaagaatatg agacaggaat ggttctacca
    39361 gattttctgg aagaagagct ttctgtttac tatgctggag tttcctcaaa taacggcaaa
    39421 ctagttggag caggaggacg tctttattta gtagaagcat acggtgagac gatcaaagaa
    39481 gcacagaaaa aaatttacac cgcgttaatg gataaaacga cagatggaac attttatcgt
    39541 acagatattg gagaaaaagc tgccaatagc gaactgatcg tgaagtaaat cagctgcttc
    39601 catttataaa aaatacaaca aacataatcg gaagcgccga ttttagtgaa gccttttcat
    39661 aaagagtgtg gtacactttt gttgtgtata taaataagga ggagttcaga tgaaaaaaat
    39721 tcaattaggt acttctgatc agaaagcagc tgcagtgatc ttaggctgta tgcgtatcaa
    39781 caatgcggaa gatccagtaa aagtgattga gacagcttat gaaaacggca ttgatttttt
    39841 tgaccatgca gatatttatg gaaacggtga atgtgaagaa atctttgcag atgctctagc
    39901 aaaaacatca atcaaacgag aagatctttt tattcaaaca aaatgtggaa tcgttccagg
    39961 tgtgatgttt gatttttcta aaaaacatat cattgaatct gtagaaaaaa gtttgacccg
    40021 tctgaaaatg gattatgtgg atgctttact tcttcatcgt ccagatacat tggtagaacc
    40081 agaggaagta gctgaagcgt ttgatgaatt ggaacggtca ggaaaagttc gttattttgg
    40141 cgtcagcaat caaaaaccaa tgcaaatcga attgctgaaa aaatatgttc gtcaaccatt
    40201 agtagctaat cagctgcaat ttggcttgaa acatactgga atgatcgatc aagggattca
    40261 tgtcaatatg acagatgaag caagtatcga tcatgacggt agtgtacttg attattcccg
    40321 attgcatgat atgacgatcc aagcatggtc accataccag tatggatttt ttgaaggtgt
    40381 atttatcggg aacgaaaaat tccctgaatt aaaccaaaca ttagaagaac tagcagctaa
    40441 atatcaaacg acaccaactg gcttggcttc tgcttggatt ttacgccatc cagccaatat
    40501 gcaagtgatc gccggtacga tgaacgtagg gcgaatcaag gaaatcgctc aagcttcaga
    40561 aattaatatg tctcgggaag attggtacga attgtaccgt gctgctggaa acattttacc
    40621 ttaaagattg tcataagcag gtcaaaaggg ctataaatta aaaagacaga ataacttgaa
    40681 agctgttttc agcttcgaga tattctgtct ttttttattg caaatggaaa gagtatcatt
    40741 gtacggattt cgtttattag ttatttttgt ttttaaggaa aagctgtgtc cgatattctt
    40801 ttggagaaat cccttgtttt ttcttaaata aataactgaa atagttaggg tcattataac
    40861 caatttcaaa agcaatatct gaaatacgat agtctgattg ctgcaataat ttttttgcta
    40921 attcgattct ttgttctgtc agataatcga tgaatgtttg gttaaacgat tgagaaaaaa
    40981 tcgtactgaa atgagaggga cttaatgcaa cttgttgagc gacccaagtt aaggacatat
    41041 ctggatcggt aaaattttct ttgataaaat ccaatgcatg gttcaaaatt ggctgatatt
    41101 tgttccttga aggctgtgag ggcagagcag ttaagaaaga aatgaacata taaactgtcg
    41161 aacggtaaag cgagtcgtcg gcgctgattg ctaagcgttg actggtggat gtgcaatgaa
    41221 ccatttcttg cgggattttc ttttctttca ggcagacatg ctggagtagc ttagtcaaga
    41281 caaataaacg atataatcgt gttcgagttg gtgtgccttg tggtttcgtc aagtgttcga
    41341 tgtacttaga tacttcgttt ttcgataatg aagacaaatc aagtgagagg tcaaagtaaa
    41401 agacagaatc aactggaagt ttcgcttctt gaatcaaatc ctcatagctg ataatttgtt
    41461 ctttaaggga actggcggtt tcaagtatca tctgcgtgat ctcaaaagat gcaggtaatt
    41521 cgctgattct ttctgcgaca ggaccaatgg cgacagaaaa attatttttt tgtccctttt
    41581 ctaattcatg gaccaacgtt tgggctacct gataactggt ctcgatcaca ttttcttcaa
    41641 gagaatcaac tattaaaaat ttgatgaatc gagaggaaac agcagagaag atcacttgtt
    41701 gattctccct aaataaaaac ttcaaataat cactgagatg agcgtaatct tcaaagtctg
    41761 tactgacttg gtttgctgct aataatacgg tcattttttt ccctgctaaa gagcgattga
    41821 aagattcagc aagtttcaat gtttcactga tcgtaagtga gcctttgaac aaaccgttca
    41881 agaaatgatt ttttttcaat tcgaaaacaa gatcagaaga agtgtgcgtt tctttttgct
    41941 gcaaatctaa agcagttact gctttttcta ttgcttgttt caattcgcta cttttaatcg
    42001 gtttaagcag ataatcatct gcctgtacct ggattgctcc tttcatataa tcgaaatcgt
    42061 cgaatccact aataaatatg agacgagtcc aaggaagtag tttccttgct tcttttgaaa
    42121 aagtcaaccc gtccatgaaa ggcatttgga tatcagtcag aatgatgtcg ggctgtaagt
    42181 cgataattga agacagtgcc atctcaccat ctccagcttc gccgacataa gtgattggat
    42241 aataagaacc aaggacagtt atttgttccc gcaagttatt gcgaatgagg tattcgtctt
    42301 cgatgataaa aactttatac atgtttgttt cctccaattg ttttgggaga tagagggatc
    42361 atcagtgtga cagttgttcc ttctcggtat ttgctgtcaa tcgtgatagg tttttgatca
    42421 tttccgtaat aaagaaggag acgcttgttt acgttgtaaa gtccgtagcc gatactgaaa
    42481 tctgaatcga tccctttggc taactcttta cgtatttctg ccagtttttc tgcagggatg
    42541 ccaatcccgt tgtctataac tttgaattgg atcatctcat caattttgca tgcggtaata
    42601 cgaataaatc ctttgtgacg gacgtacttc gttccatggt atacagcatt ttcaatcaat
    42661 ggctgcaaga tcatttttaa aatagaaaca ggaaaaagtg tttctggtat atcgatctta
    42721 tagtcaagca tatggccgta tcttattttt aagatgacta gataatcatt gatatggcta
    42781 atttcttttt caagaggaat ccagtcttgt cctttgctta aagacgtacg aaaaaagccg
    42841 gaaagtgcat gggtcatagt cagagcttct tcaaagcagt cttgttcgat caaagaaata
    42901 atggcatcca aactgttata aataaaatgg ggagtaatct gagcttgcaa tacacgaact
    42961 tcactttgtg ctaaatggta ttgtttcaaa gcgttctccc gtaaagactg gtttagatct
    43021 tccgccattt tattcatgct agcggtaagt gtttgtaatt ccagtgtttc cggcagggtt
    43081 aaacgaaagc caaggttccc tgaagaaagt tgttctgaca tctcgatcag ctgattgatc
    43141 ggttcttgga cctttctctc taaaaagcga cgattctttt tcgcatagaa aaaaatggta
    43201 ctaagaactc caagttccac tattgttaat agaatgagcg agcggacaag tgtcgtattt
    43261 tttttgcttg ctaagtcgat ctccacacga acaaaatctt gtaagatatc tgacagcagt
    43321 tgagtgacag aatctacctg attgatgatc tcttcattgg tatccataaa atcttttgcc
    43381 aagatattat tgatcagctc gttttgataa tcttccagtg tatcgatcgt acgtagcgat
    43441 acatctaacg tactaatttc tttaagtgta tttgtattat ttttgatctc tcgaatatca
    43501 ttcctaagtt cttctaggat attttcctca ttgaactcat ctacggttac ttggccagaa
    43561 atcaagtccc acatactttc caaaactttt tgatcaacct tcaaagcaat tgcatctgct
    43621 ttttggatat ttgcgatcgt ctgttgataa atcaaaagct gttgcgaata taacagtatg
    43681 ctcagtagca aagggataac agcaaataga agcatggctt tgtttgcttg ggcaaaaatc
    43741 tgttttatgc ttaaattttt tgtcatgata cttctccagt caataatttc tggttgggat
    43801 gttagctata ttctgctggg aaaagacagt ctcggaaata aaaatatctt taggtaatgt
    43861 ttttcctgtt tgaatatttc caaaataacg agtaacggta ttggctacat accagccgac
    43921 atcaggttta cactcgacta cacaattgac gattcctgag cgtagcttct cgatcatatc
    43981 ttcttgtcca tcaatagtta caatgatcaa atcttctcca ggtttgattg atgtttcttt
    44041 tatcgcagct aaagcaccgt gagtcatttc atcactatgg ctgtataata catcgatttc
    44101 ggataaatca tttgtttcta aatatcgttt catctggtct tttcctttca tccgaataaa
    44161 atcaccgaca attcggtgaa caatagtcat cggaggttta tggtcgatgg ttccatcttt
    44221 ttttagggtt ggtttacgat tgattgattc caaaaatcct tcggagcgta gcttggtcgg
    44281 tgtggagttc tctgaaccag caagttctag gattctgatt tcctgtttct ttgaatttgt
    44341 gaagtgattg ctgacaaata aacccgcccg atttccttct gctttgaaac tggggccaat
    44401 gtgggtgacg aaaagctcgg gatcattcgt ttgaatatgt ctgtcgacaa gtatgacagg
    44461 gatccctgct cgctttgctt cttttaatac agagtcccac ccggattctt gcaaaggagt
    44521 aaaaatgatc atatccactt tataggcaat gaacgtccgt atatcttgga tttgccgttc
    44581 ctgattcatg tagccatttc ggtagagaac ttggtaatct tctttttcaa gcgcagtgat
    44641 aattgattca gtatgctttt tccgccagct actctccgtt cctgattgcg aaaaaccaac
    44701 gactagtggg tcaggtgctt cctttgtatt aggtgtacac cccatcattt ggtaagcgat
    44761 tacaacaagg gctaaaaaaa ataaaacacg tttcacttta aatcactcca tctggatttg
    44821 ttagtactat cttatcagat aaaagggaaa atcaaagtca aaaggaatca atcgaaaaaa
    44881 atccaagaaa cttaaaaaaa tccaagacaa aaaaagtaca cctaaaaaat ttaaagtaaa
    44941 tcccataaaa aaatcaatgt taacgctttc tttattagct atactcggtg tatcaattga
    45001 aatgggggat aaaaaatgaa aaacaaattt agaaagattg ttgggactat cgctattttg
    45061 accgcaagca ttctattaag tgcgtgtggc aattcaggat ctgcagatga tagtacgggg
    45121 tatgtaggaa tcgctatgcc gaccaaatca gcagaaagat ggatcgctga tgggaacaac
    45181 atggtctcgg aattagaaaa actgggttat aaaacagatc ttcaatatgg agaagataaa
    45241 gtagaaaatc aagtagcaca aatagaaaat atgattacaa aaggggtaga tacacttgtg
    45301 attgcttcta tcgatggttc ggctttaact gatgtgttag ctaaagcaaa agaagcagat
    45361 atcaaggtga tcgcctatga tcgtttactg atgaattcag aaaatgtcga ttattacgcg
    45421 acttttgata attttggagt aggtgtatcg caagcagctt atattgaaga gcacttagga
    45481 ttgaaagaag gaaaaggacc attcacgatt gaattatttg gtggctcgcc tgacgacaac
    45541 aacgcactaa tcaattacaa cggagtgatg tctgttttac aaccatatat ggacaacggt
    45601 cagttggtcg tgccatctgg acagacgagt ttcagtcaaa tcgctacgct tcgctgggat
    45661 ggttctacgg cgcaagcaag gatggataat ctgttaagcg caaattatac tgaccaaaca
    45721 ttggatgctg ttttgtcgcc atatgatcca ataagtttag gaatcatttc gtcattaaaa
    45781 ggcgtggggt atgggagtga aagtaaacct ttacctgtga ttactggaca agatgcgaca
    45841 gttgctggcg ttaaatcgat cattgccggg gaacaaacac aaacgatctt taaagacaca
    45901 cgtatcttag caaaaaatac gattgaaatg atcaaagcaa tcagtgatgg agaagaggtt
    45961 ccggtcaatg atactgaaac ttatgataat ggggtgaaaa ctgtccctac gtatttagca
    46021 aatacagtat ctgtcgataa agataattat caagcagagc tgatcgacac cgattattat
    46081 aaagaatccg atctgaaaaa ttaagaggaa ccaaacaagg aactaaagag atctaagtca
    46141 gagagaggat aagaattatg gcggattgta ttttaaaaat ggcagatatc accaaaaaat
    46201 tttctggaat ccgagcgtta gattgcgtga atctgatgat tgaacgtggg gaaatccatg
    46261 cattgtgcgg agaaaacgga gcgggaaaat caacattgat gaatgtcttg agcggcttgt
    46321 atccatatgg ctcctatgac ggggaaatct tttatgatgg aaagctgtgt caatttaaag
    46381 atttgaaaga tagtgaagaa aaaggcatcg tcatcattca tcaagaatta gcactaagtc
    46441 cttatctttc aataaaagag aatatttttc tcggaaatga acaagcagaa agaggcgtga
    46501 ttgattggga actgacagaa aagaaaacat atgaactgct gaataaagtc ggtttgtcta
    46561 tcgacccgaa tcttctagta tcccagattg gcgtagggca acagcaacta gttgaaatag
    46621 ctaaagcatt atcaaaatcc gtccgtctgt tgattttgga tgagcctacc gctgcattga
    46681 atgaagaaga aagcgcgaat ttgctgagat tgatccgaga gttgaaagag caaggtgtaa
    46741 cctccatcat tatttcacat aagctgaatg aaatcgtgga tatcgcagat cgaatcacca
    46801 ttttacggga tggacagacg attgagacac tagaaaatga aatgatcagc gaagagcata
    46861 ttattcgcgg gatggtgggc agagatttag ttgatcgtta tcctgaaaga aaaccggata
    46921 ttggagaagt ttattttaaa gtagaagact ggacggtcta tcaccctctt gatcacgagc
    46981 gaatcataaa cgaccacatc aacttgcatt tgaaaaaagg agagattgta ggaatagctg
    47041 gactgatggg agcgggcaga actgaattcg ctatgagtct atttggacat tcttatggaa
    47101 gcaagatatc cggacgtata ttaaaggaag gggcagagat atcagttaag accgttcctt
    47161 cagccattgc aaacggactc gcttatgtct cggaggaccg aaaatcttta ggactgaatc
    47221 tgctgatgga tattcgggaa aacgtgacgc tgagcagttt ggataaaata agtcaaaaag
    47281 gagtattaga taaagaaaaa gaagtaactg aggcagaaaa tttccggaaa aagatgcgta
    47341 taaaaacaaa ctccatccat caaattgtca gtagtcttag tggtgggaat caacaaaaag
    47401 tcgttttatc caagtggctg atgacacaac ccgatattct ttttttggat gagccaaccc
    47461 gtgggataga tgttggggca aaatatgaga tctacacgat tatcgaagaa atggcagcaa
    47521 atggcaaagc tgtctgtctt atctcttctg aacttcctga aatattaggg atgtgtgatc
    47581 gaatctatac gatgaacgaa ggacgtatta caggagaagt ctcgcgagaa gatgcagatc
    47641 aggaagtctt gatgaaatta atgacgaaag aagaggtggg ttaagttgga acaggcaaaa
    47701 aatcttacgg ataaaaaaga gaaaaaacta aatttgaaaa gtagagcagc tgatcttttt
    47761 ggtcgctaca gcatggtgat cattttagcg ggactactta ttgcgtttca attgatgacg
    47821 gatggtattt tctggcgtcc attgaatata actaaccttg ttctgcaaaa tagccatata
    47881 ttagtactag ctgcagggat gttattagtt gtgctcttag gtcatgtgga tttgtcggta
    47941 gggtcagtga tggcgtttgt tggtgcaatt gcgggcatga tgatggtcaa tcgccaaatg
    48001 aacccgtgga tgacagttat cgcatgtctt gcagtaggcg ggctgatcgg cgcatggcaa
    48061 ggtttttggg tagcttatgc agggataccg gcgtttatcg tgactttagc aggattgctg
    48121 atgtttcgtg gactgacaca agttgttcta ggaggccaat ctctagcacc ttttccagct
    48181 gttttccaaa aaatgtctac gggttattta ccagacttaa gcgaaggaag cagccttcac
    48241 ttcttgacat tgattttagg aatgatcatc acgatcgtgt tagttgttgg tcaatggcga
    48301 acaagaaatc gtcggaaaaa tcatttattt gaagtggaat ctccaacagc tttcttggta
    48361 aaggcggtat ttacagcagt cgttgtacta ggaataactt atatctttgc ctcttatcaa
    48421 gggtatccga ttattttgat cattcttggt ttgatcgttg cagcatatgg gtttttgaca
    48481 aataaaacag tagcaggtcg acagatttat gcgacgggcg gaaatcgaaa agcggctgaa
    48541 ctatcaggga tcaaaacaaa gcgaatcacg ttctgggtat tcgtcaacat gggagtcatg
    48601 gcagctttag ctggattagt tttagctgct cgattgaatg cggcaacacc tcaagcaggt
    48661 acatcgatgg aactggatgc aatggcggca gtatattttg gtggtgcttc gacttccggc
    48721 ggcattggaa caatcatggg tgcgattgtc ggtggcttgg tcatgggtgt attgaacaat
    48781 ggcatgtcga ttttaggcgt cggggtagac tggcagcagg caatcaaagg attgatcttg
    48841 cttttagccg ttgtgctaga cttgtataat aagaaaaaac gaacaacgta ggagaacgcc
    48901 ggttattcag aaaaaagact gtgacaagac atttgtcaca gtcttttttt cgctaaaagc
    48961 gaacattcat tgtttcgttt gtatagacta aaaataatat atgttttttt accattcgta
    49021 taaattctct ttttgctctt gatttttttg cttaattgag ttaaattaca tagggcgcta
    49081 aaaaaagcct tgattaaatt aataatgttc gtgttttgct ggatgtgaac atcaggagga
    49141 taatgtaatg aaagtatttg attacgaaga tattcaatta attcctaata aatgtatcgt
    49201 aaatagccgt tctgaatgtg atacaacggt cacattaggt aaacatacat tcaaaatgcc
    49261 agtagtgcct gccaacatgc agacgattat tgatgaaaca atcgcagaat tcttagcgga
    49321 aaatggttat ttctatatca tgcatcggtt tgatgaagca gctcgaattc cgtttatcaa
    49381 aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt ggcgtcaaga aagaggaata
    49441 ttcctttata gagaagttgg cagaagaatc gctaaatcct gattatatca ctatcgacat
    49501 cgcgcatgga catgctaact cagttattga tatgattcag catattaaaa aatatttgcc
    49561 tgaaactttt gtgattgccg gtaatgttgg tacaccggaa gccgtccgtg aattggaaaa
    49621 tgcgggcgca gatgcaacaa aagtcgggat tggtccagga aaagtatgta ttacaaaaat
    49681 caagacaggc tttgggactg gaggctggca actggcagct cttagatggt gtgcgaaagc
    49741 agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca catggcgata ttgcaaaatc
    49801 tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg tttgccggtc acgaggaatc
    49861 tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa gaatactttg gttctgcttc
    49921 agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag aaaatctgga tccaacacaa
    49981 aggatctttg aaagatacac tagtggaaat gcaacaagac cttcaatcat ctatttctta
    50041 tgctggagga agagatctgg aagccattcg aaaagttgac tatgtcatcg tgaaaaattc
    50101 gatttttaat ggagacacaa tctaaaaaat aaataaaata ttaggatgtt gtgaaaaagt
    50161 tgtcctgcat taagtaataa gaacagccaa acaaaaatag ttctttatat gttttgtttg
    50221 gctggaatta ttatcgcaga tacaagcttt tgaacatcat tattcagaat aagggactgt
    50281 gataagattt ttgtcacagt cccttatgtt ttgcaaaaaa agtttttctt ttttctcgta
    50341 aattcggtaa aatcaaaaga aaaaatagac aatagaaaag agaagcttat gtgggaaaaa
    50401 gtagagaaaa aacaaggacc gatttatcgt caaattatgg aacagattat gagagaaatc
    50461 gaaaacggaa ggatcaatcc tggaagtcaa cttgaatcag aacgaaaact agggcaactt
    50521 tttggtgtga atcgttccac cgttgtccat gcattggaag agcttaggga gcttggggta
    50581 ctaacgagca aacgtggcag cggatggttt gttaatcaga ctgaatgggg gaaattcgct
    50641 gttccaagaa tcgattggcg gcaaatgatc tctcctcgtt atgagcagac ggactggtat
    50701 gagcaaaaaa taagagaagc aaagcagatc caaaaaacat cgtttcttga tttatacgct
    50761 agcgagatgc cagatgagct tttgccgaat tttgagtttc cttcttattc tcttgaagaa
    50821 atcttagcgg aagaaaaaga aatggatctt ttaggctata ctccgttacg taacaaaatc
    50881 aaacggtatt tggaaagtaa atttgaattg gcattttccc ccaaccagct tcttgtgact
    50941 ggtggcggtc agcaagcaat ctttttgatt ttacaaacga ttctttcagc tggagagggg
    51001 atagctgtag agtcaccttc ttttttttac cgattacctc ttttcaaagc ttcaggaacg
    51061 cgtttatttg ggataccgat ggatgaagaa gggatcgact tagaaaaact gaaagaatcg
    51121 atccataaaa ataaattgaa agcagtgctt atcaacccga attttcaaaa ccctacagga
    51181 aaagtgatga gtcaaaaacg acgagaaaag ctagtagaat tgtgtcgaca gtatcggctt
    51241 ccaattattg aagacgatgt atttacggat ctttcatttc taaaccaaga agaagtcctt
    51301 cctatacgaa cgattgatcc ggataatgtg ttatacgtcg gttcgttatc tagggtttta
    51361 ggaaaaacaa cgaaaatcgg ttgggtgata ggacctgaga cttttatctc gcaactagct
    51421 caagcgcaag aaatgatgga gttttcgatg aatatcctta cacaaatcac agtggctaat
    51481 gtatttaatg aagcaattga tttgaaaatg caagatgtaa gaaaaaagtt gaaagaaaaa
    51541 agccgactga taagcgcttg gggagattca caatcgtttt tcaagcttca ttcaatttta
    51601 ggtggttact atgcctggat tacttgggat gggaaaaaac tagaccccga actagcagaa
    51661 aaagtagtag atatgggtct tggaattgca ccaagtctac tttttgggca agatataaat
    51721 ggaatcagaa tcaattacag taggctggat gaaaaccaaa taccgttgtt ttattcaaag
    51781 atgaaaattc tagaaaagtg gttaacagaa taattt
//
LOCUS       DACDSR010000010        49854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDSR010000010 DACDSR010000000
VERSION     DACDSR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.010"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..1244
                     /locus_tag="IWK15_000622"
     CDS             24..1244
                     /locus_tag="IWK15_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9546249.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1241..3961
                     /locus_tag="IWK15_000623"
     CDS             1241..3961
                     /locus_tag="IWK15_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9546250.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3945..4889
                     /locus_tag="IWK15_000624"
     CDS             3945..4889
                     /locus_tag="IWK15_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9546251.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4958..5968)
                     /locus_tag="IWK15_000625"
     CDS             complement(4958..5968)
                     /locus_tag="IWK15_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9546252.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6148..6492)
                     /locus_tag="IWK15_000626"
     CDS             complement(6148..6492)
                     /locus_tag="IWK15_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9546253.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6485..6919)
                     /locus_tag="IWK15_000627"
     CDS             complement(6485..6919)
                     /locus_tag="IWK15_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9546254.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7057..7794
                     /locus_tag="IWK15_000628"
     CDS             7057..7794
                     /locus_tag="IWK15_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546255.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7787..8998
                     /locus_tag="IWK15_000629"
     CDS             7787..8998
                     /locus_tag="IWK15_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546256.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9205..9984
                     /locus_tag="IWK15_000630"
     CDS             9205..9984
                     /locus_tag="IWK15_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9546257.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10054..10716
                     /gene="trmB"
                     /locus_tag="IWK15_000631"
     CDS             10054..10716
                     /gene="trmB"
                     /locus_tag="IWK15_000631"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9546258.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10814..12568)
                     /gene="ade"
                     /locus_tag="IWK15_000632"
     CDS             complement(10814..12568)
                     /gene="ade"
                     /locus_tag="IWK15_000632"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9546259.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12700..13629
                     /gene="cpaF"
                     /locus_tag="IWK15_000633"
     CDS             12700..13629
                     /gene="cpaF"
                     /locus_tag="IWK15_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9546260.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13586..14635
                     /locus_tag="IWK15_000634"
     CDS             13586..14635
                     /locus_tag="IWK15_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9546261.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14632..14910
                     /locus_tag="IWK15_000635"
     CDS             14632..14910
                     /locus_tag="IWK15_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9546262.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14885..15355
                     /locus_tag="IWK15_000636"
     CDS             14885..15355
                     /locus_tag="IWK15_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9546263.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15372..15674
                     /locus_tag="IWK15_000637"
     CDS             15372..15674
                     /locus_tag="IWK15_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546264.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            15691..16062
                     /locus_tag="IWK15_000638"
     CDS             15691..16062
                     /locus_tag="IWK15_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9546265.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16034..16393
                     /locus_tag="IWK15_000639"
     CDS             16034..16393
                     /locus_tag="IWK15_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546266.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16565..17572
                     /locus_tag="IWK15_000640"
     CDS             16565..17572
                     /locus_tag="IWK15_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9546267.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17597..18781
                     /locus_tag="IWK15_000641"
     CDS             17597..18781
                     /locus_tag="IWK15_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9546268.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19218..20801)
                     /locus_tag="IWK15_000642"
     CDS             complement(19218..20801)
                     /locus_tag="IWK15_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9546269.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21379..21522)
                     /locus_tag="IWK15_000643"
     CDS             complement(21379..21522)
                     /locus_tag="IWK15_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9546270.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21744..22223)
                     /locus_tag="IWK15_000644"
     CDS             complement(21744..22223)
                     /locus_tag="IWK15_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9546271.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22296..22580)
                     /locus_tag="IWK15_000645"
     CDS             complement(22296..22580)
                     /locus_tag="IWK15_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546272.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22658..22852)
                     /gene="ssrS"
                     /locus_tag="IWK15_000646"
     ncRNA           complement(22658..22852)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWK15_000646"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22924..24210)
                     /locus_tag="IWK15_000647"
     CDS             complement(22924..24210)
                     /locus_tag="IWK15_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9546273.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24348..25019
                     /locus_tag="IWK15_000648"
     CDS             24348..25019
                     /locus_tag="IWK15_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9546274.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            24985..25473
                     /locus_tag="IWK15_000649"
     CDS             24985..25473
                     /locus_tag="IWK15_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9546275.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25486..26433
                     /gene="prmA"
                     /locus_tag="IWK15_000650"
     CDS             25486..26433
                     /gene="prmA"
                     /locus_tag="IWK15_000650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9546276.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26434..27198
                     /locus_tag="IWK15_000651"
     CDS             26434..27198
                     /locus_tag="IWK15_000651"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9546277.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27431..29644
                     /locus_tag="IWK15_000652"
     CDS             27431..29644
                     /locus_tag="IWK15_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9546278.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29655..30101
                     /locus_tag="IWK15_000653"
     CDS             29655..30101
                     /locus_tag="IWK15_000653"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9546279.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30272..31531
                     /locus_tag="IWK15_000654"
     CDS             30272..31531
                     /locus_tag="IWK15_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9546280.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31620..32567)
                     /locus_tag="IWK15_000655"
     CDS             complement(31620..32567)
                     /locus_tag="IWK15_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9546281.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32961..34253
                     /locus_tag="IWK15_000656"
     CDS             32961..34253
                     /locus_tag="IWK15_000656"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9546282.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34268..36037
                     /gene="aspS"
                     /locus_tag="IWK15_000657"
     CDS             34268..36037
                     /gene="aspS"
                     /locus_tag="IWK15_000657"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9546283.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36146..37021
                     /locus_tag="IWK15_000658"
     CDS             36146..37021
                     /locus_tag="IWK15_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9546284.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37287..39170
                     /locus_tag="IWK15_000659"
     CDS             37287..39170
                     /locus_tag="IWK15_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9546285.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39245..39529)
                     /locus_tag="IWK15_000660"
     CDS             complement(39245..39529)
                     /locus_tag="IWK15_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9546286.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39519..40253)
                     /locus_tag="IWK15_000661"
     CDS             complement(39519..40253)
                     /locus_tag="IWK15_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9546287.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40382..41005
                     /locus_tag="IWK15_000662"
     CDS             40382..41005
                     /locus_tag="IWK15_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9546288.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41197..42318
                     /locus_tag="IWK15_000663"
     CDS             41197..42318
                     /locus_tag="IWK15_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9546289.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42325..45453
                     /locus_tag="IWK15_000664"
     CDS             42325..45453
                     /locus_tag="IWK15_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9546290.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45472..48666
                     /locus_tag="IWK15_000665"
     CDS             45472..48666
                     /locus_tag="IWK15_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9546291.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48812..49519)
                     /locus_tag="IWK15_000666"
     CDS             complement(48812..49519)
                     /locus_tag="IWK15_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9546292.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16248 a         8779 c        10408 g        14419 t
ORIGIN      
        1 agaaaaaaag gaggaaattt ttaatggtga aatttatcca tgcggcagat ttacatatgg
       61 atcggtcatt tgaaggactg acaactcttg ataaaactgt acaggaaaaa ctattgaaga
      121 cgaatctgac cgttttatca aatatcattg aacaggcaat aataaataac gttgattttg
      181 ttttacttgc aggagataat tttcatcaaa atcgtccttc attaaaaata cagaaacatt
      241 tttcagaaca gatgaaacgg ttagaaaaga accatatacc tgtatttata atattcggaa
      301 atcatgattt ctatcaaaaa gagcggtatt ggttctcctt tcctaaaaat gtccatttat
      361 ttacatccga aacggtagag acaaaaaaaa taacgataaa aagtggagaa actgtctctc
      421 tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt
      481 ctagagaact cacagattac catattgggt tgtaccacgg ggaacctggc gtctctcaaa
      541 aaggaaacta tgctcctttc caaccctcaa agatgcaaga aaagggatac gattattggg
      601 ctttagggca tatccatgtg ccaacggtct taaatgaaaa gcaaacaatt gtttatccag
      661 gtgcgcctca aggtcatact aaaaaggaac aagcttctac atctattcta ctagttgaat
      721 taagtaaagg aagttgccaa atacatccga ttaaagtagc agaggtctat tggaagacaa
      781 aggaaatatc attgaggaaa gcaagaacga caaaagaggt cattgttcac attcgtcagc
      841 agctttcaaa agtagaggat ggatttagtt taattgagat aaaattaaaa gattatgacc
      901 atctcaatac agatgttttg gaacggatac aatcaggaga gctattagac tatttgttag
      961 aggaattctc agataggata aatgatttct ttatttggcg tatttctctc atagagaata
     1021 ctttttatac gaagacccca cttgctgctt ctgctaaatt gatggaacag ttatttcaat
     1081 actatcagac acctcaagat ttccaacaaa ttttaaatga agtatatagt catcaagaag
     1141 cggctagaat attgagcgaa ctgccggaat atcaagaaga aacacttgaa aaagccaagc
     1201 agctgcttaa tcaagatttt ttatttgagg aggatcagga atgaagattt taaggatcga
     1261 gattgctgct tttggaaaat ggcgtcaaaa aagttttgac ttttattcag gcaatcagct
     1321 gatctatggg ggaaacgaag caggaaaatc aacgatttat caatttattc aagcaatatt
     1381 atttggtttc ccttcaaaag gccgtaaaaa aaaggattat acaccaaaag acggctcagc
     1441 ctatggcggc aaaatatggc ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta
     1501 caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga
     1561 gttactagaa aaactgatcg cacctttgac gaaagagtta ttccagcagg tatttacttt
     1621 ccagcaagag cagttgatgg aactagatca tttgagagaa aaagagctac atgatgcgct
     1681 gatttcttta ggcatcacgg gcagttctca agtattccaa aagcggcaag aatattatca
     1741 gaaagcacaa caattattca agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa
     1801 tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa gaacaggaag cttattttgg
     1861 caagttgatc caacaagaac aggaatacga gtcgcaacag cagcagttat tggcacaatt
     1921 aaaagaggcg aacaaacaat tttcattagc cagacagcaa gaacttaatt tttctttata
     1981 tgaagaatgg caatctctca aaaaaaacag cactacaggt cttcctgatg agcaacttct
     2041 tttagatctg gagtctttct ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt
     2101 agaagcaaca ctggaaaagc atagcgggat ggatcagcag tcagccagat attattttta
     2161 tttagagcag gagcaggaac taaaagatct ccagcaactt caatttacag cggaacaatt
     2221 aaacgaagaa tggcaaagat tacaaattga gactagagaa acaaattcag aattgaagat
     2281 tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga
     2341 agaatggaag catcttctgg ctgaagaaag aaggattgct gatctagaaa gccagatttc
     2401 tgtgcgtctc caaatagcta aagaacagca agagatgatt gaagcagaag tgaatgactt
     2461 ggaagagacc tatccagcat tattacaaaa tcagtcagag atagcaccag tctcttcatc
     2521 taaaccgtat atgctgatgg ctggcggagc aataatcatt gcaggtatat ttgtaccaat
     2581 gcccttgaaa gtgatccttt tactaattgg tgcaggcatg attggctatt tcttctacaa
     2641 aaggcaacca aaaattaaaa ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga
     2701 aaaactagga caacttgatc tagccttagc tcaattagct gaagcagaag aaaaacaaaa
     2761 gagaactgga gaagaaaaac agattcttca gcaagaagta tatcgttttg cggatcatcg
     2821 gcacttagga aagatggata cgctcaaact catgaaagaa tatggacagg aagtcataga
     2881 ttatcagaaa ttatatggcg tatttgagcg gaaacataaa agagaacagg aaatacaaga
     2941 aaaactacga caaatcgacc aaaaatttga gtttgttaaa gaatggttac cgatacaaga
     3001 taagacaatt tcagaaaaaa tggtattgct ttcccgtttt atacaagaga tggaacagat
     3061 gaaatttgcc agaagctatc agcaaaatac tttattgaag caagaaatca atcaattgaa
     3121 aaagaaacaa aaagtatgga tacaagaata cagcgaacaa ttgactcaag caggcttatc
     3181 ttatccttcg gaagtacctg tctttttaca aaaagccaaa caagccgaac agcagatgaa
     3241 aagatacaat gaattatcgg aaatgcttac tccactattt cctgaagagc tgacaaaaga
     3301 gaaattatct gtaagactgc gccaactgaa agaaaagcaa tcccaactcc agcaagaaga
     3361 aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga
     3421 cggcacattg gacgaacttt atcaacaagc tagccggcaa aaagcaaaat tacaggaatt
     3481 actggaggaa tggggagcac ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga
     3541 gatgagtgaa agacaattac cccagttgtt aagagcagca ggtgattatt taggtatttt
     3601 aacaaaagga aattatcaga agatattact gaaaaatgat acactgactt taacagacgg
     3661 atatacagaa tttcccatct atgagttatc tacaggtacg aaagatcagc tgattatggc
     3721 gatgagattt gcttacctct cattagaagc taaccgttcg atatgcccga tcatcattga
     3781 tgatggctgg cttcattatg atcatcagcg aaagtatcaa ttagctgaac ttttagctaa
     3841 atttggagaa aagtatcaaa taatttgttt ttcttctgat aaagaaatgg taagctatta
     3901 ccatgagatg aaccaaatcg taaaacaatt agaaggagca aataatgaaa aaaattcgtg
     3961 atttagtaat cgatgagcag tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa
     4021 tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca agatacttca ggtactatcg
     4081 atggtaaata ctgggatgct tcggaggagg aaatcaataa atttacagcc ggcacagtcg
     4141 tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc tcaagtaaag attcttcata
     4201 tgcgcttaac aagggtagat gagccaggag agcctagttt atacatggaa agagcccctt
     4261 tgaagaaaga agaaatggaa gaagaaatca atcaattgtt gtttgagatc accaatgcgc
     4321 attggaatcg tattgttcgc tatctactta ataaatatca aaaacaattt tttgagtttc
     4381 cggcagcaaa aagaaaccat catgcatttg ccagtggatt agcttatcat actttgacaa
     4441 tgcttcactt agggaaatca atcgccgatg aatatcctga gctgaatcga gcattgttgt
     4501 atgctggtat cattttgcat gatttaggaa aggtgattga actttccggt cctatgtcga
     4561 cagaatatac gcttgcagga aatttgatcg gtcatctggt attgattgac gaagaaataa
     4621 ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga agatgttctt gtattacgtc
     4681 atatggtttt agcacaccat ggcttattag aatatggctc acctgtacgt ccaagaataa
     4741 tggaagcaga aatattacat caaatcgaca atatcgatgc ttcgatgcag atgatgttga
     4801 cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg gattttcggt atggacaacc
     4861 gaagtttcta tgtaccaaaa gatttatagc aaaaatttat ttttttcatt caaaacaaaa
     4921 aaagcgagac ataagtcttc gctttttttg ttttctttta ttttgatgaa tcagttgttt
     4981 cttcagttga agattctttt gttgaatcag ttgattttgt gtcagaactc tttgttgaag
     5041 ctgtagtttc acttgagtct ttcgtacttg atgaagttgt tgtgaatgca ctcaacacat
     5101 tttcaaaggc atcatctttg atttttacat ttgcatcttt taattcttca ccgattactt
     5161 tagttgtgaa tgcattgtca cttagtttag tttcagtagc gatatctttt agttgatctt
     5221 tgtatttatc catgtcattg cctttatttt ggtttttcac cattttgaca acgtagtatt
     5281 ctgtcgcata agaagtagga tttgttgtag taataacatc ggagatctca ccgtctttca
     5341 atttgaaagc tgcttctttt acttctgcag gaatagttgt tgtcgttgag tcgaatttaa
     5401 ctttaccgcc atcttctttt gtctctgtat cagttgattt gtctttggct aatttagaga
     5461 aatcatctcc atcatccgct gattttttca catcttttgc ttcatcttca cttgataatt
     5521 tgatgatttg tgcttctact tctggatgga aagatttcca agcagttttc aaatcgtcat
     5581 ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc taaattgtta cgaatatatt
     5641 ctttataagt atctttcgta tatccagcag cttctaattg tgattcaaaa gtatcaccta
     5701 atgatttcgc ttgtttgtcg tattctgcat ccacttgctt atcggttact ttatcgccat
     5761 atttgttatt gaatacttta taaatgatca tccgttgtac taaggattgg ttcgatgatt
     5821 caagttttgc ttcatcatag aaatcagata cagtgatcgt tccgcctttc atcgttgcga
     5881 tatctttatt agtatcgcct gaacaagctg ctagagttaa aacagccaac gcactagtag
     5941 ctgccaaaat aattgatttt tttttcatta aaatttgcac tcctgtaatt agttattatt
     6001 tttatatatt aactatacca tatcccattt cctttaaaaa cgttgtcgtg gtagtttcaa
     6061 acaaaattca aaatttcatc acattcctag gaaatacgca aacttttctc acttatatag
     6121 ttaaatctgt tgagaaaata atcattatta tatttgttca ggatactctg gcagatcagc
     6181 ttttagtttt tctacttgtt cattgatttg tgtgatacgt ggttcggctt ggaatttaaa
     6241 atcttcgata tcttttttga ttgcttcttg gaaagcagga atcacagatt cagctgtttc
     6301 ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg ttatttaaat catttgtcag
     6361 agttgttgct tcttccaatt cttcgatcag tttctttctt gtttcattcc cgctcctggg
     6421 cgccaataac agacctccga cagtgccagc agcagctcca aataacagtc ctttgaaaaa
     6481 gtttttaatc atcgcaggac acctgcttta cgattgtttc ttgaatagct tttagttctt
     6541 ccgatccata actatcttga tgattgacaa agtgaataga aaaatcatcc tctttgctgt
     6601 agcgagggac taaatggata tgtgaatgaa aaacagattg ataggctagt tcacggttgt
     6661 tattcaagat attaagacct ttcatttctg gaaaagcttt ttccaatgct tgagcaatct
     6721 taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc atattcaaaa atatctgcta
     6781 catgacgttt aggcacaact aatgtgtgtc ctttcgttgc ttgagagatg tctaaaaaag
     6841 catagacctt gtcgtcttca tatattttat agcttgggat ttcttgattg atgattttgc
     6901 aaaaaataca gttttccata taaaacatcc tttctataaa gaataaatac ttcagcttaa
     6961 ctttaccata agttaaagct attgtaagga aaaaaacaaa ataccgtcat tttcttcgtg
     7021 gtataataaa taaaaataaa tggcggagga aaaagaatga gtttaattat cgatcatgtc
     7081 acaggggggt acggacatct ccctgtgtta aaagatatca gtttcgatgt acagcctgga
     7141 gagatagtag ggttgattgg actgaacgga gcagggaaaa gtacgaccat caagcacatt
     7201 atcggtctgc tggaagcttc aaaaggaacg atagaaatca atcatctatc cttaaagaaa
     7261 aatacggaag aatatcgcaa acaaattggg tatattccag agactccttc tttatatgaa
     7321 gaattgacat tgagagaaca tatcgaagtc actgcgatgg cctacgatat acctatggat
     7381 atagcaatga aaagagcaga aaagttgttg gagacttttc ggttatctaa tcgtcttgac
     7441 tggttcccag ctaatttttc aaagggaatg aaacagaaag tgatgatctt atgcgccttt
     7501 ttgatcgagc cttctttata tatcatcgac gaaccatttt taggcttgga tccactagct
     7561 atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag aaggagccgg tattttgatg
     7621 tctacgcata ttttagctac tgcagaaaaa tattgtgatc gttttgtggt cttacataac
     7681 ggagagatac gagcagacgg ttctcttgag atgttgagaa aagaatttca gctgccggat
     7741 tcttctttgg acgaaatcta tattgcttta acaaaggagc aggcacatga gtagtttttt
     7801 tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa tatatgcgtt atgtattgaa
     7861 cgatcatttt gttttagttt gtcttttctt gattggcggt gtgggattat attattctaa
     7921 ttggttgaaa accctgcagc caccgtttgc aattggaggg ctcataatct ctgtattttg
     7981 gatgctctgt ttattcgtgg gtaagatggc tacttttgca gaggcagcag atattgtttt
     8041 tttacttcct aaagaaaaag aaatgcaaac ctatttggaa cgagcttttc gctattcttg
     8101 catttttccg ttgatctttt tgatattggc atgcggattg gccatgccat tagtggtggt
     8161 tagtacggag caatcctttt cgatgttttt cgtctatctg atcatattat ggagtttgaa
     8221 attcagtcat ctgcaaatca agagagcaga attgtttcga ctagatcaac aagttattcg
     8281 ttattggaaa ttaggctggt ttttttcttc tctgatcttt ttgtttctat cattttattg
     8341 gctaatatgg gcaggaatag caggtgcatt agtacaggca agtctatatt actggttttt
     8401 gtggaagcaa caagaaaaag gattggattg ggagaaaatg gtgcatgtag aagaacatag
     8461 gttgcatcag atctatcaat ttatcaattt attcacagat gttcctgaaa ttacggcaaa
     8521 gatcaaaaga agacgctatt tggatcctat attgactcat attaaaagag aatcaaaaaa
     8581 cacttatctt tacttgtttg caagaagatt ggcaagaggt tctgatttta gtggtcttta
     8641 tttccgatta gtaattatag gtgggttatt gattgccgga gtagtcgact tttattttag
     8701 tctgattatc ggtttcttat ttttatattt gatcggtttc caactgttac ctttagcgaa
     8761 ccagttccgc tatatggttc tcgttcagct ttatccaatc cataagaatc agaagaagca
     8821 ggcgattcag tatctactca catgggtatt aattatcgcg tcggttattt ttggtttgat
     8881 tgctgcagtc gtattagatg ggcagcagcg atgggttccg ctagcggctt atttgattgt
     8941 tactatgctc tttgttaaac tatatgtacc aactcgtttg aagaagatgt cagactgaaa
     9001 acaaagctgt gtagggtaaa aatgagaatg aaaaatagag cgttgacagt aagaatgctg
     9061 ttcgttctat tttttttgag aaaagctggt tttttgaatg gcaattaaat gaaaaagaac
     9121 gtatatgtgc ttgacgtgta gtgttcaggc gactagaata ataagtactg caaaacaaag
     9181 agtaagagga cgggagaatg catgatggag tttcagttag accaagagtg gcggttacgt
     9241 cccatcaaag gcgacacagg aaaaacatac ataggcatgc gtgatgagga caaagtcttt
     9301 attaaacgaa acacgactcc gatgttggct gctctttcaa gggaaggaat tacaccaaaa
     9361 cttgtttgga caaaacgaac ggggaatggc gatactttga cagcacaaga gtggctggaa
     9421 ggtcgcttgc tgactcctaa tgagattggt caaagaaacg acgtgattga tgttttgtac
     9481 catttacatc attcaaattc tcttaaaacc atgctgaccc gtatcggtgg tcgtgtcatg
     9541 acgccggaga agatgctgca aaaatatgaa aaagaattac cggaagaact ccagaaaaat
     9601 caatttctgc aaattgtcca tcgttttctt cgtgagcaga tcccgtcaat agataaggaa
     9661 aacttatcag tagtacacgg agatgtaaac catcgtaatt ggttagtatg taaaaattat
     9721 ttatatctag tcgattggga ttcaatcatg tttgcagacc ctgcggtgga tattgcaacg
     9781 attttaggaa attatgtgcc actgtctagc tggaatcagt ggctgatcag ttatggcata
     9841 cgcccgactg acgaaatgct tgaaaaattt cattggtatg cggccatgaa tttgctccaa
     9901 gaaatcacca gatattgcaa acgtgaagat catcgacgga tgaacgaaga aattttacaa
     9961 ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt tggccagacc
    10021 tttttaatta gaggaaagga atggagataa ataatgcgtg tccgaaatcg tccgggagca
    10081 gcggaaatgt tagcggctca cccgaatttt gtaattagtg atccaacatt gtggaaggga
    10141 aaatggaatg aattgtttga aaacgatcat ccaatccaca ttgagattgg tatggggaaa
    10201 ggccagttca tcactggaat ggcaaaagcc catcctgaaa tcaattatat tggagtagaa
    10261 atgcaggtca gtgttgtttc gattgcacta gacaagttga tcgaacaacc gcttcctaac
    10321 ctgaaacttc tacatgtgga tggatcagcg ttgacagagt atttcgctga cagcgaagta
    10381 gatcaaatct atctgaattt ttccgatcca tggccaaaaa aacgtcatga aaaacggcga
    10441 ttgacatata agacttttct tgctgtagat gaacagattc ttcgtccaaa tggagaaatc
    10501 catttcaaaa cagataatca aggattgttt gagtattctc tggcaagttt ttctcaatat
    10561 ggcatgatcc tcaaacaggt atggttagat ttacatcaaa gtcaatttga aggcaatatt
    10621 atgacggagt atgaagaaaa attttcttca aaaggtcaac gtatttatcg agtagaagct
    10681 caatttcaag ataagaccaa aaagaaaagt ctgtgaaact aactttctgc tttcataaaa
    10741 acattaatat ccataaataa atctcagaaa atgaacatta cagatgtaat gtattttctg
    10801 ggatttattt gatttaaaat gtatttattt catcggtttt tccacaaggt ggaatcaatt
    10861 ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt caattcgggt atgacagata
    10921 atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt agctgctcct agttgatgtg
    10981 ctttagttat taattcattc aactgttcat agacgatcga aaaaggttcc tgtgtcatga
    11041 ttccgcctat aggaagagaa acacaagcta tttcctgctg tttgttgaca acggtcatcc
    11101 cgccaccttt ttgaatcaaa agattagcag catacagcat atcctcgtcg tttgtcccaa
    11161 cgacaactag attatgcgag tcatgggcaa tcgttgtcgc tatcgctcct tgtttcagct
    11221 gaaatccttt cactatgcct aatccaatgt ttcccgtttg atggtgacgc tcgatcaccg
    11281 caatctttaa ttggtctttt tcgattgaag gaatgaatcg ttcgccttgt cggtccactt
    11341 tttctaccaa atctttagtc actagactat tcgggataat ttcgatcaca tgggcttgtg
    11401 aagaagaaag aagcagctct aacgaatgtg gatttaatgg ttgagcgttt agttttggca
    11461 gacaacacgt atatgaattt gaatgatcag caaataaatg gtcttggagt tgcccagatt
    11521 gaacaactaa tgttccctta tagaaaactt catgaattgg cagcttggta agatcgtctg
    11581 ttaagaagaa atcagcttga tacccagggg caatcgcacc tagtgttttc aaatcaaaac
    11641 attctgctgc attcaatgtt gccatctgaa tggcttgtaa tggatcgatt ccaagtgcaa
    11701 tagcttgacg gacgatatga tcgattgatc cttcttttac taaatcattg atcagtttat
    11761 catcggtgac aaagaagcat cttcttgaat tttcaggtgt gatcgccggt agtagcgatg
    11821 ccagatcttt tgcaacggtc ccttctcgaa ccattaaata cataccaaga tccaaacgtt
    11881 ctttagcttc ttttgcagtt gtagcttcat gatccgtccg aatacctgct gcaggataaa
    11941 catttaactc ttccatgccg ataccagctg catgcccgtc aatttttttc ttatgctggt
    12001 gcatcaattg gatcttttct aggatgtctg tttcattatt tgctactgct tgatagttca
    12061 tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa aggggctaat ttttttgcat
    12121 ttaaatttgc accattggtc tcaaaagggg ttacaggtac acaagaaggt agcataaaaa
    12181 agatattcat gagtgtttgt cttgcatctt ctatcataaa ctcaagacct gtactcccag
    12241 caatgtttcc tatttcatgg ggatctgtca ctacagtagt aactccatga agcaaggata
    12301 cttttgaaaa ttcgcttggt gataaaagtg agctttcgat atgcacatgg ccatcgatga
    12361 atcctggcac tagatattgc ccattagcat tatgtacgct ttttccttcg tattcaccga
    12421 ttcctacgat cattccttcg cagattgcta catctttttc catgatttct ttcgtaaaga
    12481 cgttgacgat cttagcatgc ttgatcacta ggtcagcagg ctttttttta cttgctactt
    12541 cgatatattt ccttaatgtt gtttccaatg gagtgcctcc caagataaat ttttataagt
    12601 ataacataaa ttcggctttt gccaatattt ttttgtaaat tcattttatt tgaaacaaaa
    12661 agatcaaaaa gacgtatgtt ttttgtggag gggagaataa tggaaataaa acgattatcg
    12721 gatcaaatga taagtgaaag ttttgccaaa catgcccaag acgtatatat attgccagct
    12781 tcagaagtat tttacatcta ttttcgctat ggaaaaaaac gaacattctg gaagaaagtc
    12841 tcttcggcag atggacagca actgatctca agatttaagt atttgggaca gatggatatc
    12901 ggagaaaaaa gaaaagctca gttaggtgca tttacttatt ctttagaaaa acaggaagtt
    12961 cgtctgcggt tgtcttctgt aggagattac cttcaacgag agagtttagt gattcggcta
    13021 ttatacggta cttttgaaac accccatcgt tgctttagtt ctcgagattt tgcaactatt
    13081 ttacagaaaa ccgtcagacg tggattatac cttttcagcg ggacagtcgg atcaggcaag
    13141 acttctctaa tgtatcggtt ggctttggaa gaaaaattgc aagtgattac tatcgaagat
    13201 cctgtagaga tagaagaacc gttttttctg cagctacaga tcaatccgaa aatagcacag
    13261 acttatgata cacttttgaa actcgcatta cggcatcgtc cagacttgct aatcattggc
    13321 gagataagag atgaatcaac tgcacatgct gcggtaagag cagcactaac agggcaccgg
    13381 gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat tggtcagaat ccatgaactg
    13441 acaaagaaaa aagaagagct atctgaatgt ttagctggtg tcatttatca agaacttttg
    13501 gaagatgcca atcaccatcc ttctgtttta tggagttatc aatttatgga ggaagaatca
    13561 gtaaaaaaat catgggagga aagttatgaa gaagctcagg aaaatatttg gcagtcagaa
    13621 ctattctaaa ggattaacaa gaagccagca aaaccagttt gcacaaacca tgggagaact
    13681 gcttttatct ggttttagtc tgcaagaatc cctttattta ttactcacga tccgttcgtt
    13741 tccacctgtt attttggcaa atatccaaca tcagctaaag gaagggcaag cgtttagtga
    13801 ggtactttcg caaataggat atggagcaga gcaactggta cagatcgagt tagctgaacg
    13861 tcatgggaat ctgccgacaa ctttgttgga aattgccaaa cagtttcggc ttgttgaagg
    13921 atttcgcaat gatttgaaaa agctgcttgc atatccgatg ctattgctca cctttttgct
    13981 aggtatcttg tttagtatgc ggttgttcgt tttgcctcag cttattggtt caggaatggt
    14041 catgcaagag cattggggta ttcagctgat acaagtcttt cattggtatg ttctactatt
    14101 catcctagcg gttactctat tggctttact agtaaggtat cgtctgatga agttgtctcc
    14161 tgttgatcag agtgaatgga taagcagaca attattgatt ggaccgatat attccttgta
    14221 tcaatcggct tatttgtcac tagagattgg aaagcttttt catgaaggtt tcgaattaag
    14281 gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt ctgattcagc atcttgcctc
    14341 taagttgatt caaggattgg aggcaggtat gtcattggct gagcagttcc agaattatcg
    14401 ttttctatca gaagaattca gtaaaattgt tttacaggga gaggcaaagg gaagtttagg
    14461 taaagaactg attttttaca gcgaatggac aagaaaacag cttttcaaac gtcttcatca
    14521 ctggatgcat tttattcagc caatcatttt tttgggcgta gcggctttga ttttattagt
    14581 ttatgccgct gttttattac ctgtttacgg aagcatagag gaggttttgc catgaaaaag
    14641 agaaaaatat cttattatag cggttttaca ttgatcgaaa tgctaatcgt cttactgatc
    14701 atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc gttataggaa tcatgtagac
    14761 caagaaagtc gtgaagcgat catccagttg gtagacacac aaaaagagct ctatgcgctt
    14821 caaaataatg gaagagttcc tactgtggag gaattgttga atgaaggcta tatcaaacga
    14881 gaacatgcag aaatctatca gcggccttga aggtaaggtt ttagcatcag gatatacgtt
    14941 gtttgaatca ctcttactga ttttattact tacaacgatc ctttcttttc cagttctcgc
    15001 attttctgca tggaagaatg aattggcagt ctatcaattt ttcggccaat ttgaaaaacg
    15061 aatctattct acgcaaaaaa tcgctattgt gaatcagatc caaacaggct tttattggaa
    15121 tagtgaagag aatcaaatca tttttcatgt tccgaatcca gataaaataa attgggaagt
    15181 attagatatt cctgatgaga ttaccttgaa acgccatgct tctattactt ttgctgctag
    15241 aaccgggaat gaaagttcac taaaagctta tcaattttat tgggaagaaa aagagcggac
    15301 gattacgtat caattccaga tagggagtgg tcgctataca aaaagaattg aataaaacag
    15361 gatttgtttt gttggagtcc cttacagcgc ttatgatcgc ttctgtctgt gtctacttag
    15421 tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa aagtggggag gaactgctat
    15481 ttattcgaat gatttatgaa gatatcaaga cgcagcggat gtatcacaaa agaccagagc
    15541 gaagggaaga aatgatccca tacgatatcc aaattgtcga taataatgga gtaatcgaaa
    15601 aagtgtttgt caatatggag aaagaagcga tcagtattga aaaaacaagt aattccagca
    15661 tttacgctga ttgaatgtct aattggactg atgatattga gcagtctatt gctcacattc
    15721 aatcttttga ttcagcaaac catccaagta agtgaatttt tgcagcgtaa agatcaaaaa
    15781 gagtggctaa tatttttagc acaattagaa gaagaactca agagcagtca caatgtctct
    15841 gtgaaaaatc agcaactgca ctacacgatc gaggataaac agtatatcat cgaaagatat
    15901 caggcaatga tacgtaaaag aagaacttcc ggcggacatc aacctatgtt aacaagtatc
    15961 actgagcttc aattattgga aaaagaacac acaatttctt tttatgtaca ctttgaaaat
    16021 ggagaagatg gatatgccat atggacaaaa aacgatcagt agaaaatgtt ataagggctc
    16081 tattttattt tctgtcttgc ttttatttct tttatgtacg tttttattga tggcagcatt
    16141 agaaagctac cgtttgtcaa tggatcttaa tatacgaatc aaaaattatt atatagcaaa
    16201 aataatgaag cagatggctt tatcggaaca gtctacgctt gccgaaagtt cggaaggagt
    16261 tttttactac acaactggat ctgtaacata tcagtgggta caacagtccc tattcttaga
    16321 ggtggaagtc tcaccgttta tctttcggtt cacagaagaa gtaaaaaatg atacagatac
    16381 tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt tctgttataa cactgttttt
    16441 aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg aaaacgattg aaattttgtc
    16501 aattgttcga tagaattaaa caggtgaaaa aattgttata atatatctag aaatgaggtg
    16561 caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa aacctggaag ctattcagct
    16621 tttacagaat gcattgggaa cttccttttt agaagcctat gtcgaaaatg cagaaaattt
    16681 aatagatgat tatcaagtac gtgtggtgga tggagtgccg acaaaagaga caacgcaacg
    16741 gatcacagct ctttatgaag agttgaagaa actttctttc gagccagaag aatggcgcag
    16801 actgtctcaa ttacttttat tgaagggcag ccagacagaa catttgcagg cgaaccatca
    16861 actcactcct gatagtatcg gttttttatt tgtttttctt attgaacaac tatacacaaa
    16921 taaaaaagca ccggttaaga ttctggatat tgcggcagga atgggcaact tgttattaac
    16981 agttctttta aacctatcga atgcggggta ccaaacagaa ggaattggtg tggatattga
    17041 tgatacattg ttggctgtcg cagcttctac aagtgatttg acacaagcaa atgttcaata
    17101 ctttcatcaa gacgggttgc aggaattatt aatagatcct gtcgattttg caatcagcga
    17161 tttgccaatc ggttattatc cgaatgacga aaaagcaaaa gagtttttaa caagtaccga
    17221 agaaggccat agttatgcac atcatttatt gctagagcaa tcaatgaaat atgtgaagcc
    17281 ggatggtttt ggtttgtttt tgatgccgag tggattttta gaaacggatc aaagtgaaga
    17341 aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga atgattcaat tgccagatga
    17401 attgttccgt aataaacaat cgcaaaaaag tattttgatc ttacagaaaa aaggaccaca
    17461 agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct tcattaaaag aaccagagaa
    17521 ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca tctgctctgt aatcattcaa
    17581 cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat gctgggagtt caagtttgaa
    17641 atggcaatta tatcaaatgc cagaagaaac cgtagttgcg aaagggatcg tcgaacgtat
    17701 cggtttacaa gattctattt ttaccatcaa gtacggcgat aatcaaaaat tcgaacaaat
    17761 catggatatc gaaaaccacg aagtagcagt aaaaatgctg ttagatcagt tgattgaact
    17821 gcaaatttta agctcttatg atgaaatcac cggagtaggg caccgtgttg ttcacggagg
    17881 agaaacttat ggggattcag tagtgatcga tgagaaagta atggaccgca ttgctgaatt
    17941 agcagaattt gctcctttgc ataatcctgc aaacttaatg gggatcaaag cgtttaaaaa
    18001 gattttacct gacattttga gtgtggctgt attcgataca tctttccact caacaatgcc
    18061 aaagcaaaat tatttatata gcttaccggt gtcttattat gaagatttca aagtaagaaa
    18121 atatggcttc catggcacaa gtcatcgtta tgtgtcggaa cgtgcggcaa aattattgaa
    18181 caaaccgatc gaagaactaa aaatcatcac ttgccactta ggaaatggtg tatcgatcac
    18241 agcagtcgat ggaggtaaat ccatggatac ttctatggga ttcacaccat tagctggggt
    18301 gacaatgggt acacgttcag gtgatatcga tccagctgtt ctcccatatt taatggaaaa
    18361 attagagatt gatattgatg aaatgatcaa tgttttgaac aagaaatctg gtctgctagg
    18421 cctaacagga ttgtctagtg atatgcgtga tcttgaaaag aactatgaaa aagaacatat
    18481 tcgtttagct tatgacattt tcgtggaccg catccgaaaa tacatcggcg gttatgtgac
    18541 aacgatgaat ggtgttgatg caattgtctt tactgctggt atcggcgaga atgacggaca
    18601 tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt tgtgaaatcg atccagcgtt
    18661 gaatgaattg cgtggtgaag aagtagatat ctctacaaaa gaatcaaaag taagagtatt
    18721 agtgatccca acagatgagg aactaatgat tgcccgtgat gtggagcgtc tgcgcggcta
    18781 agccaaagca ttcgttagga taaaagatga aacgcatgca agagggaatt cttgcatgtg
    18841 ttttttttgc ggagaagacc gcaaaatggt aatttaactt caatttccct tcttttttac
    18901 aggatcttct tagggaattt tgcttggaaa agtggtcaaa agggaaaaga ctggcatttt
    18961 atttattgat tttcaggaaa atcccaatat tttattttca ttcatcttta taagggacat
    19021 tacatatgta aaaagaaaaa acatttttag atcagcctcg tgggctgtcg actttattct
    19081 tcgctttatg tttagctaga aaaccgatcc gaaacattca ttgatcgaat aataaaaact
    19141 tgattttcca cctgtacttt gcctgaatcg tgaaagcaca ggtatgaaaa tcaagttttt
    19201 tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc
    19261 tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact
    19321 gctaggagcg ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg
    19381 tcgaggtgat tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc
    19441 agcaacgcca aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg
    19501 ctttgataaa agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat
    19561 gtgatggcgt aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa
    19621 taaccgattt gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg
    19681 agtaataatt tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga
    19741 tctccacgat agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg
    19801 tgcaagtttt gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc
    19861 agccgatttt tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc
    19921 agttctttgg cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa
    19981 cgacgaatca tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc
    20041 agctcagcca agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca
    20101 atcaatgcac cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga
    20161 tcatgagaaa cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga
    20221 atactgttaa gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct
    20281 gctaaaattt tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt
    20341 gcttcctgac agtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat
    20401 acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc
    20461 tctaagtttg tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg
    20521 tcctgtatct cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta
    20581 agttcttgtt cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg
    20641 acaccttctc tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgatatcg
    20701 actaaggcaa ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc
    20761 ccgccgattg tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc
    20821 actcaaaaag gccatggtta tccctggcct gtgttattta aactcagatt atcatgagaa
    20881 aaataccttg agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc
    20941 cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat
    21001 tttcattact tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg
    21061 tcttttccag tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata
    21121 tttatcgatg tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt
    21181 ggaccacatc attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga
    21241 taaaaaactt tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca
    21301 gctcttctct ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg
    21361 gcaagcggta gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc
    21421 gcggtacccg aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga
    21481 tacttcatat tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc
    21541 tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact
    21601 ttttttattt tacttgaact cattgactgt tttcccttta ttttaaacat tataaagacc
    21661 gatgagtgct tgccgcgtct tttgttttta tcacggtttt ttgtgcaaaa aaacctgccg
    21721 atagttggca ggtcgtctac gtgttaagct ttggtgtggt ctttagctgg caattggttt
    21781 tctaaatcag ttcggacaac aagcacatcg caagctgcgt ttctaataac atattcagaa
    21841 acagaaccaa taaataaacg ttctacagca ttcaatcctg tggctcctaa cataatcaaa
    21901 tctacttgat tatcttctgg gatttctctg gcaataattt gttttggtga gccatattct
    21961 acgacagagg ttacattatt caagccagct ttcttcgcat tgctttcata atctgccaat
    22021 gtttgctttg ccatttcagt agcttgttct gcaagcattc catcaaagga tgaaacggtt
    22081 tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt ctccgttatt tcggatagct
    22141 acattgaccg cctttttaaa agctaattca gcttcatcag aaccatctac ggcaaccatg
    22201 atttttttgt attgttgtaa cataagcatt cactcctcaa gtcaatcgta ttgtactctt
    22261 ttattgtaga acactttagg gaaaaagaga attatttact gctttggttc agcaattttt
    22321 tttgcaaatc ggatactgaa ggtggcagaa acaataatta cgatcagtaa aaagaccaag
    22381 gcaataaagc gttgattgaa aaaagctgtt aaaataccca ttacgcctaa aacagcatat
    22441 gcagcaccgt aaaattgtag gaatccttga tttttatcat ttttgttgat caatacaaaa
    22501 aagatatcct gtttattaaa taaataatag ctaacgaata ataatactaa agtggcgata
    22561 atcattaaaa tcctaatcat gaacaaatcc tccaaaataa gcaacaaata gaaaaagcaa
    22621 tttgccaaga gcaaattgct ttattcttta aaagatggta atctccgtta atgccgttgt
    22681 ttgtcctgta gtattgatcc cgcgaattca agcaggtggg tcccatggtc gtagcttcga
    22741 actaccaaat gataaggcat gttaccagca gtgacacaag acgggtccca agatatcaat
    22801 aaaaatgttc ggtcaacaca caaagaggac aattcgaaca tcacagattt cccacctcta
    22861 tagtagcata tgaatttaaa aagagcaatg agagagctcg cagaaacaga accgttttct
    22921 tgtctagttt tttggaggat gctttttcca ctcttttata cggtaatact gttgacccag
    22981 agcctgttca tatttccctg tatcttttgg ctgataatac tgagcattct tcagtttatc
    23041 tggtaaatat tgctgattga cccaagcctg atcaaaatga tgaggatact gataacccac
    23101 gccacggttg agtgatttcg cacctttgta atggctatct ctcaaatgat caggaacatc
    23161 tccagctttg ccctctcgaa tatcagcaag tgctgcatct aatgccatgt aggcagaatt
    23221 ggattttggc gacaagcaaa gatcgacgac gacgtctgct agtgggatac gtgcctcagg
    23281 caatcccaat ttttcagcag ctaatacagc gttcactgta cgagccgcag cagcaggatt
    23341 acctaaacct atatcttcat aaccgataac catcagacgc cggcaaatac ttgccagatc
    23401 acctgcctca actaaacggg cgagataatg aagtgcagca tccacatcac ttccacgaat
    23461 cgatttttga aaagccgaaa taacgtcata atgagcatct ccatttttgt catgggtaag
    23521 agcttttctt tggacacatt cttcaatgat cgacagtgta agatgaatcc ttccttcttt
    23581 atctgaagga gtcgatagtg tagccagttc cagcccattc aatgcactcc gcaaatcgcc
    23641 gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct aactggattg cttgttgtcc
    23701 aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc aactggatgt cttgttctgt
    23761 taatggtttg acttcaaaga tctgtgtacg gctgcggatc gctggattga tggtaatata
    23821 aggattctca gtagtcgctc cgatcaagat gattcggcca ctttctaaat gaggaagcag
    23881 aaaatcctgt tttgttttat ctaatcggtg tacttcatcc aataagagga tcactgtacc
    23941 actcattttt gcttcttccg ctacaacttg tagatccttt tttgaatctg tagcggcgtt
    24001 tagcatgcga aatgcataat tagtagagcc ggcgatcgca cttgcgatac tcgtttttcc
    24061 agtaccagga ggtccatata gaatcatgga agagagcatg cgagcatcga ccatgcggcg
    24121 tatgattttt ccttccccga ctagatgctg ctgaccaacg acttcatcga tcgttcgagg
    24181 tctcatgcga taagccaatg gctgttgcat aggattttcc tccttcttgt ttttacgtcc
    24241 cttaattata tcatatttag caagatgcct agaaagaaca gccttctgtt gttttgtttt
    24301 catagcttat ccaactaata ttgtgtatga taaagtaggt gagaaaaatg acggataaac
    24361 aaaagataga tgaatttttt tggaataaaa cgacgccaga agtggcacgg gatttattgg
    24421 ggatgtattt ggagcatgac acaccagaag gaagattagc tggatatatt gtagacgcgg
    24481 aagcatacct aggtccagaa gacgaggccg cccatagcta tggtttaaga agaacaccgc
    24541 gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct ttacacgatg catactcatc
    24601 ggattttgaa catcattact cagccagaag ggattcctca aggagtaatg atacgtgcaa
    24661 tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa actggtccag
    24721 atatcagcaa cggtccggga aaattagtgg aagcattagc aattcctcaa gaactttatg
    24781 gtcagtctat agcagatagt tcgttacgct tagtttttga gaaaaaaaag acaccaaaaa
    24841 agatcaatgc tttgcctaga atcggtatcc caaacaaagg tgtatggaca gaaaagccgt
    24901 tgcgttttgt tgtgtcggga aatccttata tctctttaca aagaaaaaac caaatcgaga
    24961 aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc tattaacata tttagatgaa
    25021 ataattgaaa aacaagtgac agactatgat gtagctttag attgggatac aaaaaatcat
    25081 acgatcgaga tcgttgttcg cttgtttgca gaaaataaag cacaatttga gattgatgat
    25141 gcagaaggga tcgtttcgca agaaccaatt atcgaatttg aagacggcgt acttcttttt
    25201 aatcctcaga aatctgtttt tgatgaacgc aattatctag cagtaatccc ttatgaagga
    25261 aaaaaagggt tggcaaaatc agtagctgat tctttagtgg aatatctaaa tgaagtatta
    25321 gctcaagggc aaagcgattt gcttgatttt ttggatgaag acgatgacga agctgtattc
    25381 gagcttcact gggataatca acaactagca gaattagtag aagtaaaaca gcaaaatgaa
    25441 gcagatactt atttaccgta cccaagttat taaggagtga cagatatgaa atggaatgaa
    25501 gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg ctaatatttt gatggaggca
    25561 ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg aacattttca gtcggatcaa
    25621 tatggtgaga ttttagataa aaatgatttt acatcgctga aagaaggcgc actagtcatg
    25681 gcttattttc cggaaacgac tttcttaccg gaaatcttgc cttttatcaa agatcgtatc
    25741 acacaattac ctgagtttgg tctagctatt ggaaaaaata aagtaacagt tagtgaagta
    25801 gaggaaagca actgggcaac agcctggaag aaatactatc atccggtgcg tattacgcga
    25861 atgctgacgg ttgcgccaag ctgggaaaac taccagacag aagatcctct agaaaaaatt
    25921 atcacactag atcctggtat ggcatttggc acaggcactc atcccacgac ttccttgaca
    25981 ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt gggaactggg
    26041 tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa aagatgtcta tgcctttgat
    26101 ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga aatagccaaa
    26161 gatgtccatg tatcagctaa cgatcttttg acaggaatcg agattgaaag tgatgtaatc
    26221 gttgcgaaca ttttagcaga tatcatctta ttgatgatcc cagatgcttg gcgtttactg
    26281 aagcaaacag gcacgctgat cgtttcagga atcattgaag agaagaagca aatggttctg
    26341 gatgccatgt tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa agactggtat
    26401 gcaattattt tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt aaaagaatca
    26461 tatgaatcaa aagactattt tgttttagaa ggggaaaact atcaccacat tgtacgggtt
    26521 atgaggatgg aacccgagga tgaagttttt ttggcattca atgatcaaat agccattgtt
    26581 gcagaaatca cggagattac tgaagaggct gtttatctga aagaaattaa aaaagaagaa
    26641 aatcaaaagg aactgccaag ttcgattacc atagcgagtg gctatcctaa aggggaaaag
    26701 ctggattgga tcgttcagaa atccactgaa ttaggagcgc aagcttttct tggtttcccg
    26761 gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca acaacggctt
    26821 gaaaagattg ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc atcagtagag
    26881 ttattggaaa cgaaacaaga tttatttgag cggctagatg attatgacgt ggtcttggtt
    26941 gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat tcttagtgaa
    27001 acaaaaccag atagtcgaat cttagctgtc tttggaccag aaggtggatt atcccgcgat
    27061 gaagtcgatt attttgtaga acacaaaggg atactttgtg gattaggacc aagaatcctt
    27121 cgtacagaaa cagcacctct ttatttatta agtgctgcta gctatcaatg ggaattaatg
    27181 aatccttctt tcagctaacg ttcttcatta ataaaagagt atcgacagaa agaattgaaa
    27241 aaatagtcaa caatttgtat aatagaaaca attatttata caatggatac gaaaaatcgt
    27301 ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg cacggatttt
    27361 gggacaatta tcaaacagat atttgtcccg attcccgttt taccgaaaaa agaaaggggg
    27421 atcgattcct gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa agctcgttag
    27481 tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat acgcgactgc
    27541 tgcacatgac ggacagttta gaaaatcagg cgaaccctac attattcatc ctatccaagt
    27601 cgcaggtata ttagcagatt taaaaatgga tccccataca gtggctacag gcttcttaca
    27661 tgatgttgtt gaagatacag aaatcacttt agaagatctg agagaagaat ttggcgatga
    27721 cgttgctatg ttagtggacg gcgtaaccaa attagggaaa ataaaatata aatcccacga
    27781 agagcagcta gcagaaaacc accgaaagat gctgctagca atggctcaag atttacgagt
    27841 tatcatggtc aaattagctg atagactaca taacatgcgt acgttgaagc acctgcgaga
    27901 agataagcaa agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc ttgcacatcg
    27961 tctagggatc agccggatca aatgggaatt agaagataca gcacttcgtt atctaaatcc
    28021 aaaacagtat taccgtatcg tccacttgat gcagacgaag agagaagaac gtgaaaaata
    28081 cgtaagtggt actgttgaag atattcgaat agcgacagaa gagctgggga tttttgcaga
    28141 aatctatgga cggccaaaac acatttattc aatctatcgt aaaatgaaag atcagaaaaa
    28201 gcagttcaac gaaatttatg acttgctagc tattcgagtg atcgtagatt caatcaagga
    28261 ttgttatgct gtattaggag caatccacac aaaatggaaa ccaatgcccg gcagatttaa
    28321 ggattatatt gccatgccaa aagctaatat gtaccaatct ttgcatacta ccgtgatcgg
    28381 tccggcagga aatccggtag aaattcaaat ccgaacacag gaaatgcatg aaatcgctga
    28441 attcggggtt gctgcacact gggcctataa agaaggaaaa aatgaaaaag tagaaccaga
    28501 tggtatgacg aaacaattaa gctggttcca tgagatactc gaacttcaag acgaaagcta
    28561 tgatgcttct gaatttatgg aaggcgtaaa aggagatatc tttagtgata aagtctacgt
    28621 cttcacacca aaaggagacg ttactgagtt accaaaagga tccggaccat tggactttgc
    28681 atacagtatc catacagata tcggtaacaa aaccactggt gcaaaagtaa atggcaaaat
    28741 ggtgcagctt gattacaaat tgaaaaacgg agatatcatt gagatcatga cttctccaaa
    28801 ttcatttggc ccaagtcgcg actggttgaa attagttgct actagcaaag caagaaataa
    28861 gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag gccacgaatc
    28921 cgtggtcaaa tgtattacag atctaggatt tacgcctaaa gatattttga cgaagaacaa
    28981 actgcaagaa gcactcgatc gttttaatta tcaaacagaa gatgatctct atgcagctgt
    29041 agggtatgga gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa aagaacgtaa
    29101 agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa atcatgaatc agccgaaaaa
    29161 agaacctgac aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag gggtagaaaa
    29221 cttactaatt cgtatcagtc gctgctgtaa tccgattcct ggtgatgata tcgttggtta
    29281 tatcactaaa ggcagaggga tatccattca tcgtcgagat tgtccgaatg ttcagcctga
    29341 caaaccaaat gtagcagaac gtttgattga agtcgaatgg gaagatacat cgaatacacg
    29401 aaaagagtat gatgcagatt tggaaattta cggctataat cgttcaggct tattgaatga
    29461 tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac agcgttgaag cacggacgaa
    29521 taaagataaa atggcgacga tccatttgac ggttggtatc cagaatttat cccacctaaa
    29581 gagtatcgtg gataaaatca aagcagtacc tgatgtctac agtgtacgcc ggacgaatgg
    29641 ataggaggac agatatgaga gcagttgttc aacgggtaag taaagctgct gtgtcagtcg
    29701 atcagcaaga aattggaaaa attggctacg gcctacttgt tttattaggc attcatgaaa
    29761 ccgatacgca aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg cgtatttttg
    29821 aagatgaaca aggaaaaatg aatattagtg tagaagatgt gggaggacaa attttaagta
    29881 tctctcaatt cacactgttt gcggatacga aaaaaggaaa tcgaccaagc tttgtggaag
    29941 ccgcacgacc agaaacagct attcctttat acgaatcatt caatgaagga atcagaagtc
    30001 gcggcatcac agtcgctacc ggacaattcg gagcagatat gtcggtttct ctcgtcaatg
    30061 atggacctgt aacgatcatt attgattcta aaaacaaata aagtaatctg gttttcacta
    30121 gtaaaaacta tgaaacacgt cgcttaagtt agactgataa actctaactt aagtggcttt
    30181 tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag cttaaattat
    30241 acaagttatt ttttatagga ggttgctgaa gatgaaagaa gtaccagtag tagatatcaa
    30301 aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg agttacgcca
    30361 tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta tcacgatcta
    30421 cggatcatca gcagcaaaag tattttatga tccaaggaat ttcaaacgaa aaggtgctat
    30481 gccaaaatta gtattgaaga cactttttgg tcaaggtgga gtacagactt tggatggagc
    30541 tgctcatcac catcggaaaa atatttttat ggatctaatg acaccggaaa gaatggaaga
    30601 ttatcatcgt atcttagata agaatctgac acaagcactt gaagcacagc atggtcaatt
    30661 tgaactattt gatttaagta agatggtttt ctttacatct atttgcgagt gggcaggtat
    30721 caatttatca gcgatttcta aagatgaggt cgaaaaatta gcagaatatc aaatctctat
    30781 gatcagcgga acgtttactt ctccgattga tcacataaaa ggtgtcgaga atcgcaaaaa
    30841 atcagaaaaa tgggcgcaag gcttgattga agaagctcga caaaatcctg tggctggaaa
    30901 agaaaatgtg gcattatatg cttttgccaa tgccacagat ttagatggtc agcttttacc
    30961 tctggaagtg gcagctgttg aattattgaa catcatccgt ccaacggttg ctctaactgt
    31021 gtgggcggca ttgatgggac atgcactttt cagtagacca gatttgtatc agcaattaaa
    31081 aaatgacttt tcgacgttac aagatccgtt tattcaagaa atgagacgct attatccttt
    31141 cttcccaatg ctacctgcga tttcgctgaa agaagtagaa gtagatggtt atcgtatacc
    31201 agaaggcagt tgggttattt tagatttata tggaacagac catgatgaac gaacagttga
    31261 agcgccagat tcctttatga tcaaacggta tgtagggaaa gcaaaagata tttcctataa
    31321 agaagaatat gagatgattg cacaaggcgg gggaaatttt cgacaaatgc atcgctgtgc
    31381 aggagaatgg atcactcttc atagtctacg tgtgttttcc gatcagctag taaataagtt
    31441 tgagttctct gtacctgaac aagattggac gattccattc aatcagtttc cgacttatcc
    31501 gaatagccga gcgctacttt acaagaatta gattagcata aacttatcga aaaaaaggaa
    31561 gatcaaacag tgctgatctt ccttttttca tattactata aattgataag gtcaattgat
    31621 caaaatccac gaaatgcttc atcaatggtt tgataatctt cattggataa atgcaaatct
    31681 aaagcttgga cattgtcaga tacttgctct ggacgcttag ctccaggtat aactacagac
    31741 actcgtggat tcttcatgta ccaagctaaa acaagttgcg caggagtggc ttggtatctt
    31801 ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt ctctgaaacg ttctccttgg
    31861 aaatctggat ttctttttcg aggatctcct tcaccaaatt gtttttcctc accaagttca
    31921 tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact gattttgttt
    31981 ttttcaagat aagggaataa ttctttttca gcctgccgat ggatcaaact gtatttatct
    32041 tctactacat cgacatatcc atctgcattc gcttctttca attgttctaa cgtgaagttt
    32101 gacacaccga ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt agcaactgat
    32161 tcattttttg gtgtgctttc atccggaaag tggatataaa aaatgtcgat gtaatcagtt
    32221 tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa actcaggaga attatcaaat
    32281 gttcgccctt tatttggtac atgagcagct tttgtagcga tgacgacacg tgagcgatca
    32341 tattcttttt ccttcagtac ctcgccaatc agttcttcgg aacggccgtt tccgtaagca
    32401 taagcggtat cgatcaaatt gatcccgcta ttcaaagctg tacgaactac ttgttttcct
    32461 gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc tcctaatcca
    32521 agctgttctg cgtagacttg tgaatgtccg attcttattt ctgccatagc tgattcctcc
    32581 tttttgtcta gtctaaaaca aaataccaaa aagctgaact aaaacgtttt tgtgagggga
    32641 aatagtcaga tatctcttga ctttgagact ggtttttagt aggttataag taatatcgaa
    32701 tgtcaatgaa agagcgagta agtttttgaa actttacaag agaggatcat cgaggctgaa
    32761 aatgatctaa agtttcatta gcctaaagac actctggaga tggacgagtg aaaagccgtc
    32821 ccgcgtacga gcgttattcg taagaggaaa gagaaatctt tcaaaattag gtggtaccgt
    32881 gtatgaattt cattcaccct tgttcatgat gaacaagggt gattttttta tttacatagc
    32941 taagaaaggg agagaaaact atgagtttcc aaagaccaaa aggaacaaat gatattttgc
    33001 caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc acgtttatta ttcagagatt
    33061 atcagtataa tgaaatgcgt acaccgattt ttgaacacta tgaagtgata tcccgaagtg
    33121 taggggatac aacggatatc gtttcaaaag agatgtatga tttttatgac aaaggggaac
    33181 gccatgtgac gcttcgacca gaaggtacag caccaatcgt tcgttctttt gttgagcata
    33241 aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt ttatatggga ccaatgtttc
    33301 gctacgaacg ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga gtagaagcat
    33361 ttggatcaga aaaccctgca actgatgtgg agagcatggt gatggcactt gatttcttca
    33421 aacagttggg gatcacgcaa atccggctag tcatcaattc attaggtgat aaagaaacgc
    33481 gtcaagccta tcgtcaagca ttgatcgact atcttttacc ttttgaatca gagctgagtg
    33541 aagactctaa acgccgcctt catgaaaatc cattacgcgt attggacagt aaagacaaac
    33601 gagatcaaaa atttgtggca gaagctccat ctatcttaga ttatctaagc gagcctgctc
    33661 aaaaacattt tgataccgtt gtttccatgc tggatgcatt ggctgttcct tatgaaatcg
    33721 acagtaatat ggttcgcgga ctagattatt acacacatac gatttttgaa atcatgagtg
    33781 acgcaccaaa aatgggggca caagcaacga tttgcgcagg cggacgttat gacaatctag
    33841 tggaagaact aggtggtcct tcaacacctg gcttcggatt tgcaatgggt atcgagcgat
    33901 tactcttgac tatggaggca gaggaagtag ttatccctgc atttaatgag ttggatgctt
    33961 acgtggtcgc actcggagac gaaacaaata tcgaagcatt gaaagtggtc caagcgattc
    34021 gaaactttgg tttttcagct gaccgcgact tcatgaaccg taaagcaaaa gcgcaattta
    34081 agacagcaga taaagcgaat gccaaattag tattggtgat cggtgaagat gagcttgcta
    34141 gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt ccattagaag
    34201 agatatatga acattttgat gaagtctatg atgaaatgac tttattcgag taggaggaaa
    34261 aaaataaatg gcacaaagaa cgatctattg cggattagtt tccgaacaac aattagaaca
    34321 aacagttaca ttaaaaggat gggtacaaaa aagacgagac cttgggggag taatctttat
    34381 cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa tcaacaaaga
    34441 agcatgggaa atcgcggata aatgtcgcag tgaatacgtg atcgaagtga ctggggaagt
    34501 tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc actggtgaat tcgaagtgat
    34561 tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa ttgaagatga
    34621 tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat ttggatctgc gtcgtccaaa
    34681 aatgactaaa aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc attatttaga
    34741 tgataatgac tttctcgata tcgaaacacc ttacctaggg aaatctacac cagaaggcgc
    34801 aagagactac ctagttcctt cacgtgtcca tgctggacat ttctatgcgt tgccgcaatc
    34861 tcctcaattg tttaaacaat tgctgatgaa tgctggattc gaccgttatt atcaaatcgt
    34921 tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag cctgaattta cgcaagtaga
    34981 tattgaaaca acgttcttaa cagcagaaga aatccaaact tacacagagg gattgattgc
    35041 gaaggtaatg aaagaagttc gcggtatcga agtcacattg ccatttcctc gtatgaccta
    35101 tgatgaagca atggctcgct acggcagcga caaaccggat acacgttttg atatggaatt
    35161 gatcgatcta agcgatacag tcaaagaagt agaattcaaa gtcttccaaa tggctcttga
    35221 aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct actcacgaaa
    35281 agacatggat cagttaggtc aatatgtagg acaattcgga gctaaggggc ttgcatggct
    35341 gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa ttcatgggag aagcaacaga
    35401 agcaatcatt aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg gcgcagataa
    35461 aagtgaaatc gtcgctgctg cattaggtgc tatccggaca cgtttaggaa aagaattagg
    35521 actaattgat gaatcgaaat tcaatttctt atgggtaact gattggccac aatttgaatt
    35581 ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc caaaagaaga
    35641 agatatcccg ttattggctg aagaccctgc aaaagtctat gcagaagctt atgatatcgt
    35701 cttgaacggt tatgaattag gcggaggttc tatccgtatt catactcgtg agcttcaaga
    35761 aaaagtacta gaaactctag gctttacaaa agaacaaatg gaagaacaat ttggtttctt
    35821 gttaacagct ttagattacg gattccctcc acatggcggt attgcattag gattggaccg
    35881 ttttgcaatg ttattagccg gagaagataa tattcgtgaa gtcattgcct ttcctaaaaa
    35941 cggaaaagca gcagatgtga tgagtgatgc accaagtatg gtttcgcctt tacaattgtt
    36001 cgaattgaat attgatgtaa cgaatataga agaatagccg ataaaaaggt aaaaaggatt
    36061 tgtcgaatac gcttcttttg acaaccttga tttaattgaa aaacacttta taatgaagcc
    36121 ataacatata aagcggggct tttccatgaa aaaaacattc gaaaattggg cttatcatga
    36181 ttacacgacc aagctttcgg tatctatcgt ttatgcgttg atcgcatcag tcgcattgaa
    36241 tttcttttat cagccaggac atatttattc aagtggtatt actggtctag ctcagatttt
    36301 gacgactttg tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa ctttgtattt
    36361 actgaatata cctttgtttg tattggcgtg gttaaaaatc gggaagaagt ttacaatttt
    36421 cacttttatc acagtcactt tgacctccct ttgtatccat attttaccgg aaacagtcct
    36481 atctaccgat ccaatcatct gtgcgttgtt tgggggggct gtgatgggac taggtattgg
    36541 gctagcgttg agaaacggcc tatcatcggg cggtctagat atcgtgagta tcacgattcg
    36601 taaaaaaaca ggaaagtcga ttggttctat ttcgatctac ttcaatgcat tgatcgtttt
    36661 tgttgccgga attctatttg gctggcaata tatgttttac agtacgttgt ctattttcgt
    36721 cagtggaaaa gtaactgatg tcgtttatac taaacaaaag aagatgcagg taatgatcgt
    36781 aacgaaacat ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta gagggatcac
    36841 gatcatcaat gaagcagaag gtgcttataa gcatgataag cagacagtat tgatgacgat
    36901 cgttacacga tacgaactgc ctctacttag agaagcaatg aaagcttctg atccacaagc
    36961 ttttgtaagt attgcagaaa acgtccagat acttggtcgt ttctatgagg atggcattta
    37021 gatcgagttt gacaaaaatt catgaaatac tgctgtagac agacgttcgt ttgtctgcag
    37081 tttttttcta ccatagtaga atcagcaaag aaaaactgct tttttatttg aaattttctt
    37141 aaagttgaaa aaaagcatgt ttattttttt aaatttatgg gatgatagaa aaaaatagta
    37201 gagaaaggac acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa
    37261 aagtttatga aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct
    37321 acatcactgg gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc
    37381 ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg
    37441 gttatttgat cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt
    37501 ggcaatttta tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga
    37561 cagcttcggc ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg
    37621 tggtcagcag cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt
    37681 tcgatcgatt tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc
    37741 aaaactattt ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa
    37801 aatggatctt ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct
    37861 tgtatacacc aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt
    37921 ctatatggtt gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca
    37981 tcccgaaaca agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga
    38041 ttctttgctt cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc
    38101 tagtgagtct gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga
    38161 atcgatggct gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc
    38221 tgtatcagtt tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg
    38281 ttttgttgca tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc
    38341 gatatgtgga gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag
    38401 gatggttcag caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac
    38461 ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc
    38521 aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca
    38581 attcagaaaa caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca
    38641 aggcacaagg gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt
    38701 tggatgctgc aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg
    38761 gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga
    38821 aagacactgt tctgattgga ctaggaacga atggctcatt ctcggaagct cagtttgata
    38881 gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga
    38941 agcgctggca aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata
    39001 tgacgctgat tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc
    39061 aagtccatcc aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc
    39121 taggaggaag ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa
    39181 aaacttcagg caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta
    39241 acattcatat agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt
    39301 gttttcgggt gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa
    39361 atacttctgc atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac
    39421 cagtaccatt gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa
    39481 atgtctgatc tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca
    39541 cgaatctctg cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg
    39601 aatccgtcta atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca
    39661 ggttttgggt aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga
    39721 atgatttcta agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt
    39781 agcttacttg aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt
    39841 gccaggtgtt tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta
    39901 cagacgacta agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg
    39961 gtgtggacag tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct
    40021 gctagacgag gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa
    40081 agtccaactg cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg
    40141 gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata
    40201 atcttgatat cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc
    40261 ctccaaaatg aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt
    40321 ggcttgtcaa acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac
    40381 tatgttttat cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa
    40441 tgcccgctat gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc
    40501 ccatcgtact tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag
    40561 ctttatggca aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc
    40621 caatgctttt ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa
    40681 gattttgaaa tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac
    40741 agaacttaaa ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc
    40801 tgtatatcaa ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg
    40861 tttcggcgaa ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga
    40921 gatcgaacga cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt
    40981 caaatatgaa gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc
    41041 tactgtcctg ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc
    41101 ctctttttgc gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt
    41161 tataataatt agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat
    41221 tggcatatcg gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta
    41281 tcttctatac tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac
    41341 ttatatgata ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc
    41401 gattggaatt taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt
    41461 acgagacttt cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag
    41521 ctagcagaag catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac
    41581 tttgaaccaa ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa
    41641 gcggtagcca aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta
    41701 ttgattagcc atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa
    41761 gtaggcgggt tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta
    41821 ggacatttgc ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca
    41881 ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca
    41941 acaggcctgg agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca
    42001 gcgagtttca aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac
    42061 atctatatct atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa
    42121 atatatcctc gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc
    42181 aaacgaaaag tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa
    42241 atgacggaag agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa
    42301 atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt
    42361 ccttttatca atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc
    42421 agcgggaaga caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt
    42481 ggtgaaactt ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca
    42541 gacgaagaaa caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa
    42601 cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc
    42661 aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt
    42721 acagaagcag atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa
    42781 atcgttttac taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa
    42841 gagactgttt tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta
    42901 aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt
    42961 ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt
    43021 ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa
    43081 gctctaacgg aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa
    43141 agcttgaaaa aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct
    43201 gctcaaaaag acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa
    43261 cgattagtcg aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca
    43321 caaaaaagta gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg
    43381 caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa
    43441 ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac
    43501 gcagaacaat taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa
    43561 aatctcgcta tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa
    43621 aaactgcaag aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa
    43681 gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc
    43741 aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa
    43801 gcagaacagc tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg
    43861 cgtccagggg agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa
    43921 cacgaacagc caaataagga agatatctta aaagtagaac aacaactggc agaagctgag
    43981 tctgctgtag aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt
    44041 caacaacaat tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa
    44101 caagaagaac acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa
    44161 acaaaaacaa ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc
    44221 aatgaaagaa tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta
    44281 gctgtctaca aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa
    44341 atcaaaagtt tagaacaaca attgcaagat acggatagta actgtttaga agaggagata
    44401 cgacgacttg aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca
    44461 ggagtgcaat tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa
    44521 caaatagaaa aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta
    44581 gaaagccagt cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca
    44641 caatataaag aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct
    44701 gaccggctgc gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag
    44761 atcagccaac agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac
    44821 ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat
    44881 cacacaagcc agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc
    44941 aacggagaaa atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa
    45001 gaagtgttac aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt
    45061 gagctagcgg ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat
    45121 gatgatcagg caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt
    45181 gcagcacttt ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta
    45241 gcaatcgatg cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa
    45301 atggcaatgg aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt
    45361 catgtaagag aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc
    45421 ggacaaagca aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg
    45481 agtattccag aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta
    45541 tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg
    45601 tatatggttg atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg
    45661 gaggaacata aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat
    45721 ctatctcgta tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag
    45781 ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg
    45841 caacctttga ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag
    45901 ctttctattt tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat
    45961 atcgtaaaaa acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt
    46021 aataaacagt accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata
    46081 ctcacgcatc ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa
    46141 gtcaaaggaa aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg
    46201 ttagagcaga tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa
    46261 ggaatacaat tttctgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa
    46321 gattatttgc tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga
    46381 gttatccaag ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg
    46441 aaacaattga ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg
    46501 tctttgttta aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct
    46561 gcagtagaag aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca
    46621 aaaggaaaaa tccaagctcg agtcgatttc gtctatggtg aagtcatcta ttcgactgac
    46681 ccgaagcatg aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag
    46741 aaacgagtac ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa
    46801 cgtccattgc caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc
    46861 cgtcagtttg gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat
    46921 aaacatcaac cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat
    46981 gtgacaggga tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat
    47041 gctttttata cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa
    47101 acaagccaag tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat
    47161 gtacctaaaa atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt
    47221 agtgaatcgt tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag
    47281 cttcccaaag gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg
    47341 aagatgttag gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa
    47401 acactacaga caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct
    47461 ttgatcgtag cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc
    47521 ccttctttaa gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct
    47581 aaagacacag atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat
    47641 cagtcgcaaa aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt
    47701 acgaaaactg cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg
    47761 acaccaatcg agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga
    47821 tttttcccga ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt
    47881 cgaccgttta ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca
    47941 gagatgaatc tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg
    48001 cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc
    48061 gggatacttg ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt
    48121 gaagattacc aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc
    48181 aaagagaaca aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg
    48241 caagaagagt tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca
    48301 caagatcggt taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt
    48361 tccttaaagg ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat
    48421 gacctttggt ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga
    48481 caaaaaaatg tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg
    48541 gattcactcc agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa
    48601 cagttgacga aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag
    48661 gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat
    48721 tgtttatttg atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt
    48781 gctatcattt ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta
    48841 acagctgatc aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt
    48901 ctcgatggtt gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt
    48961 cgtaattatc gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcca
    49021 gctgaaaaat ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc
    49081 gggaaggaat ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg
    49141 gcccatttgt gataattcct aatgggacat tcttttcctt caagaaatcg agggtttttt
    49201 tgacctcctc atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga
    49261 ggccatcttc ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat
    49321 gtgctcgcat atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac
    49381 tatgatggcg gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga
    49441 ctcttttgac cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa
    49501 acacaactga tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa
    49561 tccttgacta ttgtaacaga aaaaattgtc taaaaaagct gataaataaa ggtttttctt
    49621 actatatttc tatttccttg acagaataag gtttttatat ttcagttttt ttcactaaaa
    49681 atcgtgaaac acgtcaataa gtttgatgaa caaattaatc attatggtag aagtcgattg
    49741 atttatttta atgcaatctc tgtggaattt tcgaaagatt tattttttta atacagtctc
    49801 aatataacta ttcaaacata aaataacgtt atttgattta tttgtttata gtaa
//
LOCUS       DACDSR010000011        49582 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDSR010000011 DACDSR010000000
VERSION     DACDSR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.011"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..505)
                     /locus_tag="IWK15_000667"
     CDS             complement(<1..505)
                     /locus_tag="IWK15_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546293.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFI"
     gene            complement(604..954)
                     /locus_tag="IWK15_000668"
     CDS             complement(604..954)
                     /locus_tag="IWK15_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9546294.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1111..2640)
                     /gene="ahpF"
                     /locus_tag="IWK15_000669"
     CDS             complement(1111..2640)
                     /gene="ahpF"
                     /locus_tag="IWK15_000669"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9546295.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2650..3213)
                     /gene="ahpC"
                     /locus_tag="IWK15_000670"
     CDS             complement(2650..3213)
                     /gene="ahpC"
                     /locus_tag="IWK15_000670"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9546296.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3357..4019)
                     /locus_tag="IWK15_000671"
     CDS             complement(3357..4019)
                     /locus_tag="IWK15_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9546297.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4203..5849
                     /locus_tag="IWK15_000672"
     CDS             4203..5849
                     /locus_tag="IWK15_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9546298.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5840..5968
                     /locus_tag="IWK15_000673"
     CDS             5840..5968
                     /locus_tag="IWK15_000673"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546299.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            5935..6999
                     /gene="uxuA"
                     /locus_tag="IWK15_000674"
     CDS             5935..6999
                     /gene="uxuA"
                     /locus_tag="IWK15_000674"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9546300.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(7107..8660)
                     /locus_tag="IWK15_000675"
     CDS             complement(7107..8660)
                     /locus_tag="IWK15_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9546301.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            8808..10096
                     /locus_tag="IWK15_000676"
                     /pseudo
     CDS             8808..10096
                     /locus_tag="IWK15_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10222..11301)
                     /locus_tag="IWK15_000677"
     CDS             complement(10222..11301)
                     /locus_tag="IWK15_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9546302.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11404..11844)
                     /locus_tag="IWK15_000678"
     CDS             complement(11404..11844)
                     /locus_tag="IWK15_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9546303.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(11901..12311)
                     /locus_tag="IWK15_000679"
     CDS             complement(11901..12311)
                     /locus_tag="IWK15_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9546304.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12491..12925)
                     /locus_tag="IWK15_000680"
     CDS             complement(12491..12925)
                     /locus_tag="IWK15_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9546305.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(13025..13873)
                     /locus_tag="IWK15_000681"
     CDS             complement(13025..13873)
                     /locus_tag="IWK15_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9546306.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14262..16028
                     /locus_tag="IWK15_000682"
     CDS             14262..16028
                     /locus_tag="IWK15_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9546307.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16130..17389
                     /locus_tag="IWK15_000683"
     CDS             16130..17389
                     /locus_tag="IWK15_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9546308.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            17536..18837
                     /locus_tag="IWK15_000684"
     CDS             17536..18837
                     /locus_tag="IWK15_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546309.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            18840..19691
                     /locus_tag="IWK15_000685"
     CDS             18840..19691
                     /locus_tag="IWK15_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546310.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            19704..20519
                     /locus_tag="IWK15_000686"
     CDS             19704..20519
                     /locus_tag="IWK15_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9546311.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            20670..21836
                     /locus_tag="IWK15_000687"
     CDS             20670..21836
                     /locus_tag="IWK15_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9546312.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            22066..22713
                     /locus_tag="IWK15_000688"
     CDS             22066..22713
                     /locus_tag="IWK15_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9546313.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            22797..23741
                     /locus_tag="IWK15_000689"
     CDS             22797..23741
                     /locus_tag="IWK15_000689"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9546314.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            23876..25327
                     /locus_tag="IWK15_000690"
     CDS             23876..25327
                     /locus_tag="IWK15_000690"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9546315.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            25348..26184
                     /locus_tag="IWK15_000691"
     CDS             25348..26184
                     /locus_tag="IWK15_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9546316.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            26184..26843
                     /locus_tag="IWK15_000692"
     CDS             26184..26843
                     /locus_tag="IWK15_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9546317.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            26969..27658
                     /locus_tag="IWK15_000693"
     CDS             26969..27658
                     /locus_tag="IWK15_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546318.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            27664..28620
                     /locus_tag="IWK15_000694"
     CDS             27664..28620
                     /locus_tag="IWK15_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9546319.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            28664..29176
                     /locus_tag="IWK15_000695"
     CDS             28664..29176
                     /locus_tag="IWK15_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546320.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(29432..29980)
                     /locus_tag="IWK15_000696"
     CDS             complement(29432..29980)
                     /locus_tag="IWK15_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9546321.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(30069..31250)
                     /locus_tag="IWK15_000697"
     CDS             complement(30069..31250)
                     /locus_tag="IWK15_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9546322.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(31486..32994)
                     /locus_tag="IWK15_000698"
     CDS             complement(31486..32994)
                     /locus_tag="IWK15_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9546323.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            33153..33344
                     /locus_tag="IWK15_000699"
     CDS             33153..33344
                     /locus_tag="IWK15_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546324.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            33547..34464
                     /locus_tag="IWK15_000700"
     CDS             33547..34464
                     /locus_tag="IWK15_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9546325.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            34457..35530
                     /locus_tag="IWK15_000701"
     CDS             34457..35530
                     /locus_tag="IWK15_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546326.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            35678..36196
                     /locus_tag="IWK15_000702"
     CDS             35678..36196
                     /locus_tag="IWK15_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546327.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            36177..36569
                     /locus_tag="IWK15_000703"
     CDS             36177..36569
                     /locus_tag="IWK15_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9546328.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            36645..37580
                     /locus_tag="IWK15_000704"
     CDS             36645..37580
                     /locus_tag="IWK15_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9546329.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            37713..38492
                     /locus_tag="IWK15_000705"
     CDS             37713..38492
                     /locus_tag="IWK15_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9546330.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            38485..38802
                     /locus_tag="IWK15_000706"
     CDS             38485..38802
                     /locus_tag="IWK15_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9546331.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            39046..40656
                     /locus_tag="IWK15_000707"
     CDS             39046..40656
                     /locus_tag="IWK15_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9546332.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            40813..41661
                     /locus_tag="IWK15_000708"
     CDS             40813..41661
                     /locus_tag="IWK15_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9546333.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            41892..42371
                     /locus_tag="IWK15_000709"
     CDS             41892..42371
                     /locus_tag="IWK15_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546334.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            42404..44632
                     /locus_tag="IWK15_000710"
     CDS             42404..44632
                     /locus_tag="IWK15_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9546335.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            44761..46404
                     /locus_tag="IWK15_000711"
     CDS             44761..46404
                     /locus_tag="IWK15_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9546336.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(46477..47022)
                     /locus_tag="IWK15_000712"
     CDS             complement(46477..47022)
                     /locus_tag="IWK15_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9546337.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(47045..47530)
                     /locus_tag="IWK15_000713"
     CDS             complement(47045..47530)
                     /locus_tag="IWK15_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9546338.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(47520..48332)
                     /locus_tag="IWK15_000714"
     CDS             complement(47520..48332)
                     /locus_tag="IWK15_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9546339.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(48471..>49582)
                     /locus_tag="IWK15_000715"
     CDS             complement(48471..>49582)
                     /locus_tag="IWK15_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9546340.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        16383 a         9007 c         9580 g        14612 t
ORIGIN      
        1 tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg aatatttgga
       61 atagaagcat caaacttttg aatggattga acaataagac gaatccgcca atcaacaaca
      121 gaatactact ataaacactc caacttttag cttttgtatt catgctgaag aacaactgga
      181 acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa acgattgcag
      241 gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg aacagaccga
      301 tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc aacaacccaa
      361 gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca gaagggttta
      421 gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg tatttttgaa
      481 tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt ttaacgtgaa
      541 ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat atctctggaa
      601 aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat cgatcatttt
      661 atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc gtacaccaca
      721 aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg caaaaaattg
      781 ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat aacccgtcag
      841 ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg cttttgctac
      901 atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg ccataatcag
      961 tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa gagaagaaaa
     1021 agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca tccttttttc
     1081 cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat cgaatgctcc
     1141 taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg gactatctgt
     1201 acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct cggtcactat
     1261 ttcatttctt ggattcattt cgatcgttcc atctagccaa tccgtattag ggagtaagcc
     1321 gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac ggcgatcgat
     1381 ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg catgtttgat
     1441 aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg cttttaattc
     1501 tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga ttgctgcttc
     1561 aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata aagggccgtc
     1621 acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg gtacattgat
     1681 agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg atataccgtt
     1741 agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct gttgattcat
     1801 cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca attttggacc
     1861 ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga cttgtccgcc
     1921 gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga tagctgcact
     1981 actagctgct ggtccaccgc caattactaa tacatcataa ggttcttttt caaacaactc
     2041 ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca tgcgtccgct
     2101 actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt tatcatcgat
     2161 ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat ttaatacagc
     2221 taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc taacataact
     2281 gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt cactgatttt
     2341 cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag
     2401 ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag aaaaactagg
     2461 tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg ctatttcctt
     2521 cgtaaattca gctagttttt gactattttc actattatcc aaactaacag aaaaaacaat
     2581 aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt tgtctaacat
     2641 atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt cgccactttc
     2701 tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag cagcgcgaat
     2761 cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt ccatagcttg
     2821 aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt gctcttcatc
     2881 caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca cgtattcgat
     2941 tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg tatcggtaga
     3001 gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt gtaagtcttc
     3061 taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa tactccagtt
     3121 ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag catgtgcatt
     3181 aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa taattattta
     3241 tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg ttatttaata
     3301 aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag aaaatgctac
     3361 tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag catcatgtgt
     3421 aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa agccttgagt
     3481 aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt cactcgtaac
     3541 cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac ttcttttctt
     3601 cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg
     3661 tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac tattcgtgtt
     3721 tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg ttctcggaca
     3781 aagcgagcat tttctgcaga aacaagatta attttggaaa catacgtacc actttgaggt
     3841 atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg
     3901 ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg ttctaagtca
     3961 gcatagataa tttgtttacg aatcgcttga taagcttgtg attgcaagct gcttttcatc
     4021 tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct gaaaaacaat
     4081 ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt tgacatagcg
     4141 atttttattg tataaaattc acttgaatac aaaaactaat atgttagttt aggaggggaa
     4201 aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa aaagaagtag
     4261 aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga
     4321 tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct caacgtttag
     4381 cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat gaagaaataa
     4441 ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg ccaagcagtg
     4501 gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt catccagatt
     4561 gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta caattagtta
     4621 cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa tatataggac
     4681 cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg agtattcttg
     4741 ctgcgctact ttataaacgt tttttatctg gagcttggcc gattgcactt gtctctacag
     4801 ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc gcaaaagaat
     4861 ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat gaagaaattg
     4921 ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg tcagttagca
     4981 aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata
     5041 atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa gatcattttc
     5101 ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt gcaacggtag
     5161 ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg tctttagcta
     5221 ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat ccagatttat
     5281 cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag tctccaggta
     5341 tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct aatccaaata
     5401 ttcctgatat gcctcagcga atcgccacgg atacctctca aaaggtggcg attcgttatg
     5461 gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca cttgtattca
     5521 taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat caaggaaaac
     5581 cattcatccc gagtccagac ccactattga cagactttca gcataagctt tccgatattc
     5641 aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt ttgtcaaata
     5701 aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata gaagaaatgt
     5761 tcatcaagat ggtagcagga ccgaatgccg tacgaaaaac attacaggat tacttacaag
     5821 aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac aggaagaaaa
     5881 gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag gcacattgat
     5941 ggatcacagg aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg
     6001 attgtaggca cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa
     6061 gaactaaaag aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat
     6121 gtgcatgatg ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag
     6181 cagacaatcc gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct
     6241 gtatttgact gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta
     6301 gcttttatat ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat
     6361 tccagcaatg aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc
     6421 ttgcttgaag cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt
     6481 ctccaatcaa tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat
     6541 gatccgccat actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat
     6601 tggttgtgcc gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt
     6661 gcagaagatc cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga
     6721 atcccatttg cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca
     6781 ccgcataaat ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac
     6841 aacggattta acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga
     6901 agaccaggat acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg
     6961 gaagcaatcg aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat
     7021 tatgtaaata agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc
     7081 attcggattt atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga
     7141 tccatgagtg cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt
     7201 ttccagtctt ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc
     7261 aattgtttca aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt
     7321 attttgatcg attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata
     7381 taaaaatcac gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca
     7441 tacaaatgta ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt
     7501 aacaagtgtt gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact
     7561 tctttcaaca cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga
     7621 ttgaagttaa gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc
     7681 ttttcttttt caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt
     7741 gaataaagaa catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt
     7801 tcttccgatt cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt
     7861 tcgcgacaaa ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca
     7921 atatattcat actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc
     7981 tctttacata tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca
     8041 agtacttctt ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca
     8101 taaaattctg tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga
     8161 atttgtacct cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa
     8221 ccgtacaatg ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta
     8281 tagtttgttt gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc
     8341 aagatctgat aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa
     8401 gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt
     8461 tctgttagat tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt
     8521 gattcagaca tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca
     8581 gataggaaaa gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta
     8641 atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa
     8701 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
     8761 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
     8821 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
     8881 acacagagga gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
     8941 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
     9001 aaacagcgac catcaaagca aaaattgact attcaggaga cttagcacct atactacaat
     9061 cagctgttac atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt
     9121 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
     9181 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt
     9241 taacgccgta tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag
     9301 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
     9361 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
     9421 aaactcaacc agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag
     9481 atgtagaccc agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc
     9541 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
     9601 aggtagcccc agaagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc
     9661 aaacagtacc ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag
     9721 aggtagatcc agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc
     9781 aaggaatacc agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta
     9841 aaaaatcaaa tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa
     9901 tatttgatag cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg
     9961 tcctgaaacg aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact
    10021 gtggagcgga atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca
    10081 aaacagatct ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa
    10141 tatccagaaa atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact
    10201 ttatctttct tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat
    10261 ctttttcact ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc
    10321 ccaaacgcca aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag
    10381 ttctgttttg ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat
    10441 caaaatcgta atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa
    10501 agatagagga atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat
    10561 catgatcgct aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag
    10621 caacgtattt aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac
    10681 aaacttatcg gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa
    10741 caatcttgag aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa
    10801 tgacatagca aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga
    10861 agcgccattt tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg
    10921 taaaacagga tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat
    10981 gtcaaatgtt tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta
    11041 aacgatcagt gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg
    11101 aatgatttga acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt
    11161 aatcatactt ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg
    11221 tattttttca ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca
    11281 aaaaataaac acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa
    11341 aaaccgctaa atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca
    11401 gtttcagccc tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg
    11461 cagtgtatcc aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc
    11521 ttctgcttgt ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc
    11581 atcacccgtt tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg
    11641 agtcagcgta ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt
    11701 cttttcccat aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag
    11761 gaggggagtg tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg
    11821 attaactaaa tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata
    11881 gcggatttac acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca
    11941 gctgtagctt ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct
    12001 tccagagaaa gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca
    12061 ctttctgaat aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc
    12121 tgtttgatca attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt
    12181 tcagggttgg ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta
    12241 cgatcgggat tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac
    12301 atttttttca taaaattacc acctttaatt atattttata caattgaatt ttacacaata
    12361 taattgtgga aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac
    12421 agacgcgctt tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc
    12481 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac
    12541 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
    12601 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
    12661 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
    12721 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac
    12781 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
    12841 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
    12901 aatcgatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
    12961 agattctttt attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac
    13021 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
    13081 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
    13141 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
    13201 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
    13261 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
    13321 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
    13381 taaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
    13441 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
    13501 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
    13561 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc
    13621 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
    13681 taacgatatt caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta
    13741 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
    13801 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
    13861 tcgtttcttt catttattca cttccttaat tcattaataa tactcttatc ttattataac
    13921 ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta
    13981 tgtttcattc ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag
    14041 aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag
    14101 actaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
    14161 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac
    14221 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
    14281 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
    14341 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca
    14401 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
    14461 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc
    14521 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
    14581 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
    14641 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
    14701 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
    14761 atccagaccg agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt
    14821 acttgacgga ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca
    14881 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
    14941 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
    15001 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
    15061 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
    15121 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
    15181 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
    15241 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
    15301 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
    15361 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
    15421 tgaattatgc atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
    15481 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
    15541 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
    15601 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
    15661 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
    15721 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
    15781 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
    15841 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
    15901 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
    15961 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
    16021 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
    16081 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
    16141 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
    16201 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
    16261 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
    16321 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
    16381 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
    16441 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
    16501 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
    16561 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
    16621 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
    16681 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
    16741 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
    16801 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
    16861 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
    16921 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
    16981 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
    17041 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
    17101 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
    17161 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
    17221 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
    17281 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
    17341 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
    17401 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
    17461 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
    17521 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
    17581 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
    17641 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
    17701 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
    17761 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
    17821 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
    17881 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
    17941 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
    18001 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
    18061 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
    18121 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
    18181 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
    18241 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
    18301 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
    18361 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
    18421 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
    18481 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
    18541 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
    18601 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
    18661 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
    18721 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
    18781 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
    18841 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
    18901 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
    18961 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
    19021 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
    19081 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
    19141 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
    19201 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
    19261 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
    19321 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
    19381 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
    19441 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
    19501 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
    19561 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
    19621 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
    19681 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
    19741 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
    19801 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
    19861 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
    19921 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
    19981 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
    20041 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
    20101 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
    20161 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
    20221 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
    20281 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
    20341 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
    20401 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
    20461 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
    20521 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
    20581 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
    20641 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
    20701 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
    20761 acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga
    20821 aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg
    20881 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
    20941 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
    21001 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
    21061 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
    21121 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
    21181 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
    21241 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
    21301 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
    21361 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
    21421 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
    21481 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
    21541 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
    21601 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
    21661 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
    21721 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
    21781 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
    21841 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
    21901 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
    21961 tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
    22021 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
    22081 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
    22141 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
    22201 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
    22261 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
    22321 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
    22381 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
    22441 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
    22501 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
    22561 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
    22621 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
    22681 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
    22741 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
    22801 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
    22861 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
    22921 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
    22981 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
    23041 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
    23101 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
    23161 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
    23221 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
    23281 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
    23341 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
    23401 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
    23461 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
    23521 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
    23581 atctgctatg tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg
    23641 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
    23701 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
    23761 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
    23821 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
    23881 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
    23941 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
    24001 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
    24061 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    24121 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    24181 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    24241 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    24301 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    24361 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    24421 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    24481 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    24541 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    24601 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    24661 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    24721 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    24781 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    24841 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    24901 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    24961 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    25021 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    25081 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    25141 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    25201 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    25261 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    25321 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    25381 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    25441 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    25501 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    25561 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    25621 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    25681 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    25741 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    25801 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    25861 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    25921 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    25981 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    26041 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    26101 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    26161 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    26221 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    26281 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    26341 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    26401 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    26461 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    26521 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    26581 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    26641 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    26701 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    26761 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    26821 gttctatgca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    26881 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    26941 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    27001 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    27061 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    27121 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    27181 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    27241 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    27301 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    27361 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    27421 gcaaatggag cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag
    27481 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    27541 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    27601 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    27661 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    27721 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    27781 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc
    27841 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    27901 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    27961 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    28021 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    28081 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    28141 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    28201 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    28261 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    28321 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    28381 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    28441 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    28501 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    28561 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    28621 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    28681 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    28741 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    28801 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    28861 tgcattagtc tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    28921 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    28981 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    29041 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    29101 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    29161 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    29221 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    29281 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    29341 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct
    29401 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    29461 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    29521 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    29581 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    29641 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    29701 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    29761 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    29821 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    29881 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    29941 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    30001 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    30061 tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    30121 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    30181 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    30241 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    30301 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    30361 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    30421 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
    30481 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
    30541 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
    30601 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
    30661 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
    30721 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
    30781 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
    30841 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
    30901 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
    30961 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
    31021 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
    31081 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
    31141 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
    31201 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
    31261 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
    31321 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
    31381 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
    31441 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
    31501 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
    31561 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
    31621 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
    31681 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
    31741 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
    31801 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
    31861 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
    31921 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
    31981 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
    32041 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
    32101 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
    32161 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
    32221 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
    32281 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
    32341 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
    32401 atgtcctttt tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc
    32461 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
    32521 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
    32581 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
    32641 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
    32701 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
    32761 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
    32821 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
    32881 aactagcttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
    32941 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
    33001 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
    33061 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    33121 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    33181 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    33241 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    33301 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    33361 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    33421 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    33481 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    33541 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    33601 aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    33661 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    33721 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    33781 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    33841 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    33901 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    33961 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    34021 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    34081 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    34141 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    34201 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    34261 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    34321 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    34381 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    34441 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    34501 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    34561 tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac
    34621 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    34681 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    34741 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    34801 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    34861 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    34921 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    34981 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    35041 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    35101 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    35161 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    35221 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    35281 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    35341 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    35401 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    35461 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    35521 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    35581 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    35641 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    35701 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    35761 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    35821 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    35881 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    35941 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    36001 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    36061 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    36121 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    36181 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    36241 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    36301 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa
    36361 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    36421 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    36481 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    36541 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    36601 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    36661 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    36721 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    36781 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    36841 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    36901 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    36961 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    37021 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    37081 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    37141 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    37201 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    37261 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    37321 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    37381 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    37441 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    37501 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    37561 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    37621 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    37681 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    37741 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    37801 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    37861 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    37921 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    37981 gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac
    38041 tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata
    38101 aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga
    38161 tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga
    38221 cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag
    38281 gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat
    38341 tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa
    38401 aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact
    38461 tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt
    38521 ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa
    38581 gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta
    38641 gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata
    38701 gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt
    38761 ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa
    38821 aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt
    38881 caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca
    38941 agctcttttt tattttagga attagagagg gatttgtcct tctatgattt tttgctataa
    39001 tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg
    39061 atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc
    39121 tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca
    39181 ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt
    39241 ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt
    39301 cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca
    39361 ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat
    39421 gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca
    39481 atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc
    39541 gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact
    39601 gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta
    39661 gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat
    39721 tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt
    39781 cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac
    39841 caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg
    39901 gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc
    39961 atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat
    40021 cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt
    40081 ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat
    40141 gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta
    40201 ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta
    40261 actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc
    40321 ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat
    40381 cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta
    40441 aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg
    40501 aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga
    40561 agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg
    40621 aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc
    40681 acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt
    40741 ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag
    40801 gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt
    40861 atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt
    40921 tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa
    40981 gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg
    41041 aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt
    41101 gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac
    41161 tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat
    41221 gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac
    41281 gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt
    41341 gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa
    41401 attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt
    41461 attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat
    41521 aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa
    41581 atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc
    41641 attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca
    41701 tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta
    41761 tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca
    41821 tatagtttag taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag
    41881 gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag
    41941 actatgataa agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg
    42001 gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata
    42061 ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag
    42121 cgatcttagc tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt
    42181 atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag
    42241 aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac
    42301 aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc
    42361 taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact
    42421 aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag
    42481 ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga
    42541 tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca
    42601 attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc
    42661 agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga
    42721 acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac
    42781 actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct
    42841 agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga
    42901 aatcgttcga gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg
    42961 agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt
    43021 accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa
    43081 ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa
    43141 aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca
    43201 aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa
    43261 agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta
    43321 taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac
    43381 tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga
    43441 acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg
    43501 aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat
    43561 tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa
    43621 acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag
    43681 cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc
    43741 tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg
    43801 acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt
    43861 ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc
    43921 acttagccgg caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg
    43981 aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt
    44041 tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga
    44101 atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat
    44161 tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg
    44221 ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg
    44281 gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact
    44341 tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat
    44401 tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat
    44461 ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg
    44521 ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga
    44581 cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa
    44641 agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa
    44701 ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat
    44761 atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt
    44821 ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat
    44881 tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt
    44941 gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg
    45001 gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat
    45061 cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa
    45121 gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat
    45181 aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat
    45241 tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa
    45301 gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta
    45361 gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc
    45421 tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt
    45481 gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca
    45541 caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc
    45601 tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct
    45661 tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt
    45721 cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa
    45781 gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa
    45841 atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa
    45901 agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt
    45961 cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct
    46021 ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta
    46081 gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt
    46141 agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca
    46201 caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta
    46261 acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc
    46321 ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct
    46381 cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg
    46441 aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc
    46501 gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg
    46561 aggtctgaca ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc
    46621 gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc
    46681 agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt
    46741 ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg
    46801 gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg
    46861 accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca
    46921 tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat
    46981 ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa accttctttt
    47041 tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa
    47101 gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc
    47161 aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata
    47221 aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt
    47281 gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga
    47341 tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag
    47401 cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca
    47461 aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct
    47521 tagttttcat ttctcattcc tcctttttga ataccgccag accagacact tccggtatga
    47581 tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact
    47641 gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa
    47701 ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca
    47761 attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca
    47821 atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa
    47881 gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta
    47941 acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca
    48001 gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg
    48061 aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct
    48121 aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat
    48181 tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg
    48241 aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga
    48301 tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata
    48361 acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa
    48421 aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt
    48481 tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg
    48541 attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc
    48601 caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa
    48661 tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag
    48721 tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa
    48781 aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa
    48841 acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt
    48901 aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga
    48961 gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc
    49021 tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga
    49081 aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata
    49141 gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca
    49201 gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct
    49261 tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc
    49321 atggacatca aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa
    49381 gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat
    49441 cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg
    49501 gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt
    49561 tccattaaat aaagaatagg aa
//
LOCUS       DACDSR010000012        47029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDSR010000012 DACDSR010000000
VERSION     DACDSR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.012"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(132..659)
                     /locus_tag="IWK15_000716"
     CDS             complement(132..659)
                     /locus_tag="IWK15_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9546341.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(726..2228)
                     /locus_tag="IWK15_000717"
     CDS             complement(726..2228)
                     /locus_tag="IWK15_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9546342.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2486..3607)
                     /locus_tag="IWK15_000718"
     CDS             complement(2486..3607)
                     /locus_tag="IWK15_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9546343.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3733..4674
                     /locus_tag="IWK15_000719"
     CDS             3733..4674
                     /locus_tag="IWK15_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9546344.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4824..5720)
                     /locus_tag="IWK15_000720"
     CDS             complement(4824..5720)
                     /locus_tag="IWK15_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9546345.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5989..7329
                     /locus_tag="IWK15_000721"
     CDS             5989..7329
                     /locus_tag="IWK15_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9546346.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7547..7990
                     /locus_tag="IWK15_000722"
     CDS             7547..7990
                     /locus_tag="IWK15_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9546347.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8494..9912)
                     /gene="nhaC"
                     /locus_tag="IWK15_000723"
     CDS             complement(8494..9912)
                     /gene="nhaC"
                     /locus_tag="IWK15_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9546348.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10315..11538)
                     /locus_tag="IWK15_000724"
     CDS             complement(10315..11538)
                     /locus_tag="IWK15_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9546349.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11569..12408)
                     /locus_tag="IWK15_000725"
     CDS             complement(11569..12408)
                     /locus_tag="IWK15_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9546350.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12425..13105)
                     /locus_tag="IWK15_000726"
     CDS             complement(12425..13105)
                     /locus_tag="IWK15_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546351.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13107..14180)
                     /locus_tag="IWK15_000727"
     CDS             complement(13107..14180)
                     /locus_tag="IWK15_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9546352.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(14827..15498)
                     /locus_tag="IWK15_000728"
     CDS             complement(14827..15498)
                     /locus_tag="IWK15_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9546353.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15585..18263)
                     /gene="mgtA"
                     /locus_tag="IWK15_000729"
     CDS             complement(15585..18263)
                     /gene="mgtA"
                     /locus_tag="IWK15_000729"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9546354.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18922..19359
                     /gene="ndk"
                     /locus_tag="IWK15_000730"
     CDS             18922..19359
                     /gene="ndk"
                     /locus_tag="IWK15_000730"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9546355.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19381..20247)
                     /locus_tag="IWK15_000731"
     CDS             complement(19381..20247)
                     /locus_tag="IWK15_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9546356.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20407..22500)
                     /locus_tag="IWK15_000732"
     CDS             complement(20407..22500)
                     /locus_tag="IWK15_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9546357.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23249..23872)
                     /locus_tag="IWK15_000733"
     CDS             complement(23249..23872)
                     /locus_tag="IWK15_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546358.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(24031..25428)
                     /locus_tag="IWK15_000734"
     CDS             complement(24031..25428)
                     /locus_tag="IWK15_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9546359.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25460..26431)
                     /locus_tag="IWK15_000735"
     CDS             complement(25460..26431)
                     /locus_tag="IWK15_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9546360.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26805..27230
                     /locus_tag="IWK15_000736"
     CDS             26805..27230
                     /locus_tag="IWK15_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546361.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27718..28419)
                     /locus_tag="IWK15_000737"
     CDS             complement(27718..28419)
                     /locus_tag="IWK15_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9546362.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28435..28875)
                     /locus_tag="IWK15_000738"
     CDS             complement(28435..28875)
                     /locus_tag="IWK15_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9546363.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28875..29450)
                     /locus_tag="IWK15_000739"
     CDS             complement(28875..29450)
                     /locus_tag="IWK15_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9546364.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(30298..30777)
                     /locus_tag="IWK15_000740"
                     /pseudo
     CDS             complement(30298..30777)
                     /locus_tag="IWK15_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30936..31637)
                     /locus_tag="IWK15_000741"
     CDS             complement(30936..31637)
                     /locus_tag="IWK15_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9546365.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(31745..33154)
                     /locus_tag="IWK15_000742"
     CDS             complement(31745..33154)
                     /locus_tag="IWK15_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9546366.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(33202..35067)
                     /locus_tag="IWK15_000743"
     CDS             complement(33202..35067)
                     /locus_tag="IWK15_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9546367.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(35188..36039)
                     /locus_tag="IWK15_000744"
     CDS             complement(35188..36039)
                     /locus_tag="IWK15_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9546368.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(36349..37062)
                     /locus_tag="IWK15_000745"
     CDS             complement(36349..37062)
                     /locus_tag="IWK15_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9546369.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(37130..37456)
                     /locus_tag="IWK15_000746"
     CDS             complement(37130..37456)
                     /locus_tag="IWK15_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9546370.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(37579..37806)
                     /locus_tag="IWK15_000747"
     CDS             complement(37579..37806)
                     /locus_tag="IWK15_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9546371.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(38128..39381)
                     /locus_tag="IWK15_000748"
     CDS             complement(38128..39381)
                     /locus_tag="IWK15_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9546372.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            39442..40182
                     /locus_tag="IWK15_000749"
     CDS             39442..40182
                     /locus_tag="IWK15_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9546373.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(40268..41026)
                     /locus_tag="IWK15_000750"
     CDS             complement(40268..41026)
                     /locus_tag="IWK15_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9546374.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(41023..42051)
                     /locus_tag="IWK15_000751"
                     /pseudo
     CDS             complement(41023..42051)
                     /locus_tag="IWK15_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(42071..45238)
                     /locus_tag="IWK15_000752"
     CDS             complement(42071..45238)
                     /locus_tag="IWK15_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546375.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(45462..46346)
                     /locus_tag="IWK15_000753"
     CDS             complement(45462..46346)
                     /locus_tag="IWK15_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9546376.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(46441..46878)
                     /locus_tag="IWK15_000754"
     CDS             complement(46441..46878)
                     /locus_tag="IWK15_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546377.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        13471 a         9868 c         8034 g        15656 t
ORIGIN      
        1 attaaaataa ttaaatttca tttctaaagg ttgagtttat attatcaaaa gaattgtcat
       61 aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat ttaaataaca
      121 caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa tgtaaagaac
      181 ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc attatggcaa
      241 actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg aacgatccgt
      301 gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc taaacgtgct
      361 ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc catttctacc
      421 aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg tgctgtatct
      481 cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa atctactacc
      541 ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac atctgcccgt
      601 ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac ataaagcatg
      661 tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc ttgagattac
      721 aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag gaaggtttga
      781 tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc taattgttct
      841 tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc agctggctgt
      901 gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg ctcgatcttg
      961 ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa tgcttggtag
     1021 tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc ataattttgg
     1081 cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac cttgccgttg
     1141 aatgccccca gaagataatg gataatggat gactttcctg caccgttagg acctgaaatc
     1201 gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag attttcatac
     1261 cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg gctattcatc
     1321 gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct gatctgggtc
     1381 tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc agcacccact
     1441 gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt ctttgtttta
     1501 tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt tagcctactg
     1561 acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag ttttttctgt
     1621 tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc gattgctaaa
     1681 acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat gaagccttgt
     1741 tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa atgattcgtt
     1801 ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag taggactttt
     1861 gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg atctgtttgc
     1921 atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc atttaacacg
     1981 taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa ttcttgctga
     2041 tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa agttgtcttt
     2101 cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt tagatttgct
     2161 tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt tatttcgatt
     2221 ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag
     2281 aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc aagccaatcc
     2341 cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc agctttcatc
     2401 aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg aaattacgat
     2461 agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg
     2521 tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct tcttctaaac
     2581 ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt ccttctagct
     2641 cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt gcaagcactg
     2701 ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct tctcctgtaa
     2761 tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg actaaatcga
     2821 aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg cctgctgctc
     2881 cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc tgagcaatct
     2941 ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt tgtcttccaa
     3001 attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca gcgataatca
     3061 attttacttt agaaacaaga acgtttcttc gatcaaaatg aagtttttta gtagataata
     3121 aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt aatcctagac
     3181 caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact tcttgaagca
     3241 gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata gtttgatccg
     3301 ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt
     3361 ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag agatccatcc
     3421 catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca gtattttctc
     3481 cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag ccatttagaa
     3541 ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa
     3601 tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat ttttttcaaa
     3661 caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca ttgaataaag
     3721 gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag ccgaatcgga
     3781 ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt
     3841 gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa
     3901 ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga cttgatcgta
     3961 gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc cattattact
     4021 gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt actggaaaaa
     4081 tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg tctagctggt
     4141 cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt ggaaccagaa
     4201 ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt aaatccgatc
     4261 tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat
     4321 ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag
     4381 agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga aggaatcata
     4441 ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga caacatcggg
     4501 ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac
     4561 tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac accatattgt
     4621 gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct
     4681 ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc aataaaagat
     4741 caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt ttcagtacat
     4801 aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc atattcgtat
     4861 tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc tgcatcataa
     4921 aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc cggtaattca
     4981 tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg ataatgagca
     5041 atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa
     5101 gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt
     5161 ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg tggaagaatg
     5221 aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc tgctcgttct
     5281 tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa
     5341 ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt tgcttccaaa
     5401 aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc
     5461 tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt ggtaaatggg
     5521 cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat cagctgataa
     5581 tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc ttttccggac
     5641 caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt tgcaactagc
     5701 agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt tcctatgata
     5761 gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga aaatttaaca
     5821 tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct aaaagttttc
     5881 tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat attgctattt
     5941 attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa
     6001 aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc
     6061 ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg
     6121 attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc
     6181 gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt
     6241 gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc
     6301 gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg
     6361 gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta
     6421 ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat cttactgatc
     6481 atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt
     6541 cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct
     6601 ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa
     6661 gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt
     6721 tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca
     6781 ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg
     6841 gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat
     6901 gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta
     6961 gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt
     7021 tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt
     7081 tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg
     7141 gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta
     7201 ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc
     7261 ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg
     7321 aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg agctattgaa
     7381 gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga
     7441 cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct
     7501 catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg
     7561 gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa
     7621 tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat
     7681 gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa
     7741 gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt
     7801 acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc
     7861 tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc
     7921 ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga
     7981 aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag ttatcagctt
     8041 caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg aaaaagctgc
     8101 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt
     8161 aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtaaaatcag
     8221 cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat tttgccattt
     8281 tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt tgtgaaaaag
     8341 tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat ttttgtataa
     8401 atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa agggattgtg
     8461 acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg ctgtcttgct
     8521 tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga aggcactgac
     8581 catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg
     8641 aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta agtagttgat
     8701 tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc ctgctttttc
     8761 ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt caccaataaa
     8821 aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat
     8881 ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca acaatccacc
     8941 taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga ttcctccgcg
     9001 gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg attcgatgac
     9061 cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc ctactgctac
     9121 agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac acagtaacat
     9181 taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta gaacatcagt
     9241 cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa
     9301 aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga tgtgtttgaa
     9361 caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg gagacatctt
     9421 atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat ttaagcctag
     9481 tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc
     9541 tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc actggacact
     9601 gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta tccatacagc
     9661 aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt tgatgccatt
     9721 gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat acaacatgac
     9781 cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga caacgccaat
     9841 cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac ttggtgttcc
     9901 tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga
     9961 tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat aaaaagacct
    10021 gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt
    10081 tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg
    10141 atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa
    10201 gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt
    10261 tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt tttactagaa
    10321 agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc
    10381 taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat
    10441 cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc
    10501 ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac
    10561 atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg
    10621 aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac
    10681 atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc
    10741 acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc
    10801 acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc
    10861 tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc
    10921 attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc
    10981 atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt
    11041 tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc
    11101 ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc
    11161 atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa
    11221 tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc
    11281 gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa
    11341 ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt
    11401 aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc
    11461 tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt
    11521 tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc
    11581 gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt
    11641 gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt
    11701 tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat
    11761 ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat
    11821 gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt
    11881 ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt
    11941 gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg
    12001 gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat
    12061 tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt
    12121 gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa
    12181 agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt
    12241 tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt
    12301 tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca
    12361 aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc
    12421 actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta
    12481 agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc
    12541 tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc
    12601 cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca
    12661 aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca
    12721 ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc
    12781 caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag
    12841 tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca
    12901 gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta
    12961 ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca
    13021 tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca
    13081 aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta
    13141 tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat
    13201 gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt
    13261 tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac
    13321 agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat
    13381 ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca
    13441 agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc
    13501 tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag
    13561 atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga
    13621 ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt
    13681 gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt
    13741 tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca
    13801 agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt
    13861 aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag
    13921 atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc
    13981 gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact
    14041 aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc
    14101 gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa
    14161 acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa
    14221 tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt
    14281 gttcacacac aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg
    14341 tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt
    14401 ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat
    14461 ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa
    14521 aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc
    14581 tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat
    14641 cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca
    14701 gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg
    14761 ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attatttttt
    14821 ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa gatcttttcc
    14881 ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga caaaactgga
    14941 atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga
    15001 atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag ctgtcagata
    15061 aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg tgtccaagta
    15121 atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc
    15181 aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg catatggttt
    15241 taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct tcgctcggac
    15301 ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac tttgtttcca
    15361 gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa attgctgaaa
    15421 tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac catctaaatc
    15481 aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca tagtttagca
    15541 cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa gagttcttgg
    15601 aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc gatgactgta
    15661 gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc agcaccaaaa
    15721 cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac aaacatggca
    15781 aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg gagcactaag
    15841 gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat gaaaatcact
    15901 cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca aataacaaaa
    15961 tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg
    16021 aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc tttatccatc
    16081 cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa caaaagttgt
    16141 aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga aaacatattg
    16201 ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa agtttctcgt
    16261 ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca
    16321 gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc cactttcatt
    16381 gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt tttacgtaaa
    16441 gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc atggtcttca
    16501 gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt aaccaattca
    16561 ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt gtccccagtc
    16621 agaactttta cagctacacc atgttctttc agagctttca acgcttgttt cgttgtttct
    16681 tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc atctttgact
    16741 gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac tcgtaagcca
    16801 tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt tgttaatggt
    16861 gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc aatcgcgcct
    16921 tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat acgacgacgt
    16981 tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt atctaaagtt
    17041 tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag
    17101 ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga
    17161 tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca taacacatcg
    17221 attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt tttggccatc
    17281 acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggtgttaga
    17341 ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt tgtaagccca
    17401 ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag catggatgtt
    17461 ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt ttctgctaca
    17521 tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact tccactgatg
    17581 acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg atcgtcttca
    17641 catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc ttgggaaatg
    17701 aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc tgctgaaagc
    17761 cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt tccattacgt
    17821 tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg attggaacgt
    17881 acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat aatggctaca
    17941 ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa tgaaataatt
    18001 cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg
    18061 aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct tgaacgttcg
    18121 atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa ttcttgtttc
    18181 gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc catctcaatc
    18241 tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc tattcatcgg
    18301 atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc tggcctttcc
    18361 tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta
    18421 attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt agaccacctc
    18481 ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc caaatactca
    18541 gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt tgagttttga
    18601 cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa gccttatctc
    18661 gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg
    18721 tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata acaactaaaa
    18781 aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata cacacgttat
    18841 attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata atagaaatat
    18901 agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc agacggcgtg
    18961 agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact ggccattgct
    19021 gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca gcacttgact
    19081 gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt cgtttatctt
    19141 gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc aacaaaagct
    19201 gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac tgaaaacatc
    19261 attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca
    19321 ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta aactgctggt
    19381 ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc gttgattctt
    19441 tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc
    19501 taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat agatcactgt
    19561 acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac gtgccttttt
    19621 caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa agagatactg
    19681 ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca aataattgtt
    19741 ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata gttcaaattg
    19801 accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa
    19861 ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca aaaccaaata
    19921 atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt tataaggatc
    19981 aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact ttattggttc
    20041 atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc gctctaaaaa
    20101 tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc cgactagaga
    20161 agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt tgactcgaag
    20221 tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa atttttccac
    20281 attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aagaataaaa aaatgttttt
    20341 ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga aggttggttt
    20401 gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg gatataagtt
    20461 ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt
    20521 ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg gatcaatcgt
    20581 ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag
    20641 acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga ttcctgctgc
    20701 tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta
    20761 tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct gagaagataa
    20821 taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt
    20881 cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag tataaccagg
    20941 catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc
    21001 tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga tcccaaaatg
    21061 atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa
    21121 aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat ctactgtgct
    21181 attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg
    21241 gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc
    21301 agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt cggattcatc
    21361 aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat gatctctatt
    21421 ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct tttgtccaag
    21481 gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata atgagccttg
    21541 aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct ttccctgtcc
    21601 tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa tactatttaa
    21661 aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct ggaaactttc
    21721 taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg gatttggctc
    21781 tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc
    21841 gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg catcgattcc
    21901 tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc
    21961 ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc
    22021 catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag
    22081 tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct tcatcaaaga
    22141 aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag atttgactgt
    22201 aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa
    22261 aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat acaaagaaag
    22321 gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt actggatcgt
    22381 accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa
    22441 gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac
    22501 gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc
    22561 ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc cgtccacttt
    22621 tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata aaagtcttgt
    22681 ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt acaaaaaaag
    22741 aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca gctttttcac
    22801 aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca caatatttat
    22861 aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa acggcaggct
    22921 caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat tatgtcagga
    22981 cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    23041 ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg gctcagagtt
    23101 aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgacct tcggtattag
    23161 ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt gtttctctaa tactcaggtc
    23221 aaaacgctga tttcacaacc tctgtttttc attgtccata ggattgatat tgtccttgtg
    23281 atgactcatt cgtaataatt gaggcttgat tttcttcact tcccgttatc gtttgtccac
    23341 tgccagaatc tgaaggactt gttcctgtcg actcgttttt ttgagaagag acagttgttt
    23401 tcttgacttg ttcatttgtc tttttccatt ccgctaaacg atcggcgacc acttttttgt
    23461 ctactttttc agaataagcg gtgatcactt ttccattttt caaacgggct actttgtcgc
    23521 catcatgagt caaaagcaaa acggtcaaat gcttgtcttt aaattgatat tgtctgagag
    23581 aaatcgcact aagtacgtca tcgatattaa taaattgatt ttcttctttt ccttttggca
    23641 aataaatgtc caattcgcct ttttcgtaat tggcagttat tttatactcc gcccaccaat
    23701 aagttgagaa ttcttttttg aaattatcta ctgattcttt gcttgctacg attttttctg
    23761 tttctttctg agcaagatct gctgtcattt ttcctgaaaa tgctgcggat aaaataagca
    23821 gtaagaaaaa accgaataac caaaagagaa gaaacggtct ttttttcctc atgctttcct
    23881 ccactcctat ttaattttga ctattctatt atatacaaaa aatgaaatag aaagacaaaa
    23941 aagttgaaaa agatcgtaaa taattgaact tctttttcac aaaatggtaa gaatggagaa
    24001 tttcacgcat ttgctctcaa ctattctttc ttaagcctca aatccgtttt tatctgatac
    24061 gtctttgaac caatgacccg attttttgat tgttcttttt ccatcttgtg ctaagtctac
    24121 tgaaataaag ccatatcgat tcttatatgc attggtccac gaccagttat ccatacatgt
    24181 ccacatatga tagcctaaac aattggcacc ttcttgaatg gcttgatgga catatttcag
    24241 atgagattgg acaaattcga tccgataatc atcgtggatc atgccatctt ctttgataaa
    24301 tcgtgcttcg ttttctacac ccataccgtt ttcagaaata aagcagtcga tatttccata
    24361 atgatctcgt acattgatca aaatatcata gattccttgt tcatagatct cccatccgcg
    24421 ataaggattc attttacgac caggcatttc ataattgtcg aaataatcct caggcattgg
    24481 accgttttta gtatcgattg gtgtttcttt tgcttttacc cggcgtggct ggtaataatt
    24541 gatacctaaa agatctactg tattttcttt gataaccgtc aaatcctctg catggatcac
    24601 aggcatgaga tcaagttctt ttactatctc aatcaattca cttggaaact cgccttttac
    24661 agctggatca aggaatgaac gattgaagaa cgcatctgct atttgcgctg ctttaacatc
    24721 ttcagggttt gattcgtctc ttggatagct tggtgtcaga ttcaagatga taccaataac
    24781 accaccttgc tccatttcat ggtaagattt gatggctaac gctgaagcca acgcttcatg
    24841 atagccaact tggatcgctt ttttcatatc tgactcttct ggataatgcc atcgatataa
    24901 ataacctgct tcgactggaa cgattggttc attatgagta aaccattttt ttactttcga
    24961 accaaacaat tcaaaacaac ttttggcata tttttcatag gcttcaaccg tttctcggtt
    25021 caaccatccg cctttttctt gcagatacat cggcatatcg aaatgataca gattcacaaa
    25081 gggttcgata ccgttttcaa tcaactcatc aaaataacga tgatagaaac gaacggcttc
    25141 ttcgttgatt gttccagatt catcaggtat caaacggctc cattggatag atgttcgaaa
    25201 tgaattatgc ccagtctgtt tcatcaactg tacatcctct tgatacttcg tataaacttg
    25261 cgaggttttt tcaggtccta cttgatcaaa gaacttttgc ggttcttttt tgtaccaata
    25321 atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt ccttctgttt gtgggccact
    25381 tgctgcagaa ccccaccaaa aatttgctgg aaattgatat tttttcatca aagcacctct
    25441 ttttcttttt tagttcgttt tagttgattc catgtatgat attttccatc acaatcactt
    25501 tttcattttc ttcctcttct tttttgattc gttttaacag ctgttctgct gctcgttccc
    25561 cgattttatc tgttggtggc gtgatcgtga ttttcggaag gatactcaga tcgtttttat
    25621 ctaaataatc atagtgactg aacgcaatat ccttcccgat tctgacacct tcttgattga
    25681 aacattcgat ggcaccaatc gacatattgt aattgctgac aaaaatagca gttgcccctt
    25741 tttctaataa tgcttttgcc ccgtaatagc cgctttttct agaatagtct cctgtaaaga
    25801 cctctaattg gatcgatgga tcattttgga ccgcttcttt ggctcctgct aaacgttctc
    25861 ttgctacgta gtctgtttga gtggcagcaa taaatccgat ttttttatgt ccttgttcac
    25921 catgatgagc gatcactttt gctacacttt ctcgatcatt cactaatatt gtatcaatac
    25981 ctgctggtcc ttctggacaa ttcaatgcga taactggaat actcattttt gtgagcattt
    26041 caaatccaga ccactcttct tcggataaga agacaatcag cccatcgatc acatgcttca
    26101 tcaggtattc aattttttgt tcaatttttt ctggctgatc ggcaaacccg cttaataata
    26161 agctataccc attttcttca gctactcttt cgatccctga gacaacttcc gacccaaaac
    26221 ggctggaaat cccgtttatc aaaagtccaa tagagaagct tcgattattc ttcaaccctt
    26281 tagcaatgat gttttcttta tagtccaacg ctgaaatcgc ttctgctatt tttttcatgt
    26341 tcttttcctt cagttgctga ccattcaagt aacgggaaac tgttcccact gaaacaccag
    26401 ccatcttagc cacatcacgg atcgatgcca tacattctca ctcctgccag ttacatttga
    26461 tttctctttc ttgttgttcg attatgatga ccgcattctt tgcatagtgt tgcttgtcat
    26521 caatatccat ataactaaag tcttcagtag agcaacttcc tctcactatt aattatactt
    26581 aaacaagtat ttgaaccggt tcatttttaa tatagcattt ttttaaaaga ttgcaacact
    26641 tttttaaaac gctttctgaa agtggcggat tttttgcctt tttaaaattt tattctaaaa
    26701 tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc atccgtttga agttttacat
    26761 tccaatggta taatttcttt atcctatcaa atgggggaag tattttgaag aaattaatcg
    26821 tactatttat tttgtctgcg ggacttttga cattgactgg ctgtgatttg tttggcggct
    26881 ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt aaaagcggaa aaagcagata
    26941 ccaccaacag tgtatatacg ctaaaagtac gtaaagatat ggatttatcc gcgggacagt
    27001 tgacatttac agataaaaaa atggaatgga cacgcacgta tcagacacaa ggaactgatt
    27061 cttctgaatc atctgaagaa aaaacagtgc tgacagatat caagatcact accaaagatg
    27121 acgaatatat tatttcagga aaagaaaata aaaaagaaac aacagtcata tttacaaaaa
    27181 tcggtaatta ccgtttgaag gatgaggatg gcaacatcta cagtctatag tgccgtctgt
    27241 tggatcttct tttgtttctc cccttctttt ctgctatagt aaagaagcaa gatgattgta
    27301 aaggagacga aatggatgat cgatagtcct gattattgta catgtaataa caacaaaaat
    27361 atccatacag aatttaatga ccaaggaagc ttgagcatat gcgacaactg tcacaaacta
    27421 gtcaaagatt catttaaaac atttgaatct tatcctgttc aaaattatgt aaaaagtaga
    27481 agaagataaa caaatccaag caaaactcac tatggctgat ttttatgaac ttcaaactca
    27541 gcttcttcct ttgatcaagc ttcatcagta aaacaaacag tggatcattt cagtttacaa
    27601 atcaaagagt aaagatttat ttatttcaaa tatttgaata taaaataacc aaacgataga
    27661 aaggcttatt ctacatcacg tagtaatttt tctttcccgt cgtttggttt tttattttta
    27721 gcctaattct tgaccttttg catgctggtt atccgtaaaa tattttccag caggctttcc
    27781 ttccaattca gctaattttt cttggatctg acggatcacc ttttttgcat cttctggatt
    27841 ttctacaaaa tcgaatttat ccccatccac ttgtatttta ggacatacat caaaatcttc
    27901 ataccattga ttatagcggg tgttcaacgt ttcataatag gtatataacg tctcatcgct
    27961 ttctagctgt tcatagtcac gcccacgttt tttgatacgt tctagcatcg tgtccaaaga
    28021 aactttgata tggaccatca aatcaggccg tttttttgga gcaacatcat ctaactcgtt
    28081 tagcatcgta tctaataagc tatcgtactg ctgtacttcg atatccgtta cccgacctaa
    28141 atcagcgttt agatggaaca acaggctatc ttcatagatg gatcggtcta gcacattgtc
    28201 atcgttcact aaagcatttt tcattgctaa aaatcgcttg ttcaaaaaga agatttgcaa
    28261 taaaaaagcg tactgctcag gatttgaata aaagagcggc aatacttcgt tatcttcgac
    28321 attttcataa aatggtttag aatcaatctc ttttgcaagc atatccgtca aactgctctt
    28381 acctgcacca atcgttcctg aaatcaaaat cactgacata caacccttcc tcttctataa
    28441 gtactcgcct acgtaagttt caacaggcaa tgtttcaaaa tcatattcac gtacagcttg
    28501 gtctgatttt aaatacgtat ggagactttg tgtaatcatc aagttcgtag gtaccgtctc
    28561 ttcttgtaca atgatcatcg gttttttcaa catagttgcc acacctaatt cgtaagccgt
    28621 tcctggatcg attgtttgat gctcaaagtc aaggatagca acaacaaatt ctgcatttgt
    28681 cagttcttcc atatcttttt cgtatacttc ctgcgcccat ccagcgctga atagttcata
    28741 attgctttct tggtgatgac gtggagagta gaaacttgtg actgttttgt tttcctccaa
    28801 cgctttttcg atacgactta ctcgatcgat ttgctcctct gaaaaaaatg gtcctgctaa
    28861 atatatttga ctcattattt tgcctccttc aataataact gatttttaat agatactgct
    28921 cgtaattcat tgaccagttc caatggattg ttggaaaaga ttacttgatc caaccatttt
    28981 ttatccataa aatcctcttt tgcaaaagtt tctaataatt gttgcaagcc gctgtaataa
    29041 ccgccaatat tcaatattcc gattggcttg tcgggcgtca gaccgattgc tatccaagaa
    29101 agaagttgtg agaattcctc caatgtacct agacctccag ggaaaacgag gacagcatct
    29161 cccttttcaa ataataatac ctttctttca tcgatcgttt ctgtaggaat aaatgtagta
    29221 acatcttttc caggcaattc agtttcaaaa agcccattcg gatagatacc tgtgactttt
    29281 ccgttatttt ccagtactgt tttactaatc actcccataa tcccggagta tgaaccaccg
    29341 taaaccagtt cgatttcttc ttgtgccatg tattttccta acgtttgcgc aataaatttg
    29401 taactttctt tattaccaaa acgggaaccg caaaaaacag taagtttcat aaaaaaacac
    29461 tccctaactc aaaagtctat cttcattcta cacgtgtttt tttaaaataa aagactagat
    29521 tttgtagttt tttagtggta tataggatag acgccactat atatagtatt tagaaaaaaa
    29581 tcatttttca gccattttat agataaaaca aaaataaata acgtgttttc atcgatatga
    29641 aatgacttac ttatagaaga tactgggatt tcgtcctata tgccaataac gtgtattatt
    29701 ttaaacaaca aatacttgtt tcttttatta acgaaaaaat cattttgttc tcgtgttatg
    29761 tttcactgat aaatgatgtt tctctaaata aaaaagccga cgaactaaac gatcaaaatt
    29821 tgatcatttt tgttcatcag cttctcctaa aaatcttttt gttaacgata aatagctgga
    29881 tttgtaaaaa tatcacttga atcgtcactg ctggaagaaa attcagtgat aaccgcacct
    29941 gttgcgcctg cctggaacta gtgtttgata ttgggctcga acgtgtactg atctctagca
    30001 tgaagaataa tttctttttt tactgtataa taactttctt tcctttttgg aatagtacag
    30061 gaaagttttt ttgtttcctt tccttcgaca taaggtatac cagacccttt cgacaacgaa
    30121 aggttctcat ttttccttca tcgtcatttc aaggagggtg ccgatgctct ggacagtttc
    30181 gatcgctcct aaaccaaaat ctgtatattc gattctttct aactctgatt ctttcatacc
    30241 attcctgtct caaggtacat ctattgtact tttttcttgt aatccattct gcctctccta
    30301 actaattgtc aaatcatccc aagatatgtc ttcttcctct ttttcttctt ggatgatttc
    30361 tgcattcgga tctactggtt ttttcaataa taaaatggtt attcctgtga caaaagaacc
    30421 agctaacaat gcaccaatga atgcccatgg tctggtcatc gtagcgactg caaacattcc
    30481 cgatgccgga atacggctat ctgcgcccca aaacatgctt aatccgccac ctactgtccc
    30541 accgactccc gttgctatga cattgcgtgc caagtcgttc aatacgatag ggaaacatcc
    30601 ctctgtgatc tgacagatcc ccataggaaa agcggtcttc aatgtatcga tttcttgacg
    30661 attgagtata tttttacgaa tgattttccc taagaaataa gcgatcgtca agccgaatgg
    30721 ggttaccatc gaagctaaaa tcaatacagt gattggttca tagattcctt ctgaaaatcg
    30781 tgccacaccc ccttatccag aataaatggt aatccagaaa cagccgtgta tctgcggtaa
    30841 taagcctaac caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
    30901 atgcagggca gctttttcac aacctctttt tttatttatg tttcataatc aatatggtaa
    30961 ggatgcagct ccgtatggta gaccgcctcc gaatattcaa tcatttcccc aatttgcgta
    31021 gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg ttttcagaat tccttgttct
    31081 tcttctgtta aaaatactgc cgagaaatca tcagaaaatg agtcaatagt atatcctttt
    31141 ttcgtcagca aacgatacaa agattctttg ccaaattctt ctctgctgaa ttgcatcatt
    31201 tcttgtggaa gataatgaac aaaataaata taaggctgat tgttcaaata atataagcgc
    31261 ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag gatcttcctt ctttatatgg
    31321 accaattgca aatccagcgt ttctttccgg acttcatatc gggagttctc caagatttgc
    31381 gtaaagcttg ccgctttgga taatcgattg taaggacgat tgcttagaac cgtcgtccca
    31441 cgaccacttt tctttgcgac atattcatct gatgccagca gttcgattgc ctttcggaca
    31501 gtgattttgc tgacttgaaa cagttcttct aattcacttt ctgtcggcaa catcgaaccg
    31561 actggatatt ttcctgagaa aatatcttct ttaattttat ctgcgacttc caagtatagt
    31621 acacgtgtcc ttttcataac gtcacccttt gctttactat tactaaaaga atagcacata
    31681 aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg ttctttttca ttcagctgct
    31741 ttaattacaa gtttacgcca tttgaggcaa ttacttcttt ataccaatca aaagattttt
    31801 tctttgatcg attcagtgtc ccatttcctt catcgtctag atccacatag ataaagccgt
    31861 aacgtttact catttctcct gttgatgcac tgacaagatc gatacatccc caaggcgtat
    31921 agcccatcaa atctacaccg tctgctactg cattagccat cgcttcgata tgacgacgta
    31981 aataatcgat ccgataatca tcaataatcg tgccatcctc ttccatatga tcaatggctc
    32041 caagaccgtt ttcaacaatg aataatggtt tctggtaacg attgtataat tcattcaatg
    32101 cgattctcag cccttctgga tcgatctgcc agccccattc acttgcctca agaaacggat
    32161 tttttactcc tcccagcaag ttcccttctg aagttgctgc ttgtggatca gtctcattga
    32221 ttgccgtaga catataataa ctaaatccga tgtagtccac tggatattct tttagcaact
    32281 ggaagtcttt ttccgtttgc tctaattttt ccggatcgac tccatatttt ttatgggtac
    32341 gcgctgtgta tgcagggtat tctccacgta cttggacatc cgtgcagaaa aaattgaatt
    32401 cttggttttg gatcattgtt gctgcctgat tgactggatt ggcatcgatg ctgtaagttg
    32461 ttgcatagat aatcatgcag ccgatttgga tatccgaacg caattcgtgg ccgattttta
    32521 cggctttgct acttgcaacg aattgattat gccatgcttg gaaaatattt tggtactctc
    32581 ctgctccatt gctttttact aatccttgac ttaaagctgg aaagtgaaag gccgaattga
    32641 tttcattgaa agtcatccaa tatttgactt tactgctgta tctctctaat actgtctgag
    32701 caaatcgttc atagaagtcg atcagttttc gatttttcca gccaccgtat tcttttgcca
    32761 aatgaagagg catttcgtaa tgagaaatcg tgatcacagg ttcaatatca tattttaagc
    32821 actcatcaat cagctgatca taaaattcta atcccgcttc gttaggagta ccttcgtctc
    32881 catttggaaa aattcgtgtc caggcaatcg aaaaacgata acatttgaat cccatttccg
    32941 caaacaaggc aagatcttct ttgaaacgat cgtaatgatc gattcctcga tgattcgggt
    33001 aaatatattt ttcttgatca atcgtccagt caaattcttc actgccgata atcgcaaagc
    33061 gctgcttacc tccaggcata gcatctgcaa cggatagtcc ttttccgtct cgatcataag
    33121 ccccttctaa ttgatttggc agcagttgct ccaccccata aaaatttttc tgaaaaacgt
    33181 gattgcatcc tgatttcctc cttattttac aattgccaga acatcttcgt ttgcaataat
    33241 ctctttttgg tctaattcaa gtatatcggc aaatgcatca gtattcgtaa tgacaactgg
    33301 agtgattacg ctatagccag ctgctctaat tgcttcgatg tcaaaacgaa tcaataaatc
    33361 tcctgctttg acgagatccc cttgtttgac taagatctca aagccattcc catttaattc
    33421 tactgtatcc atcccgatat gaatcaaaat ctccgctccg tcagtcgttg tgatccctac
    33481 agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct gcaggtgcaa ctagttctcc
    33541 tacttctgga tcaatagcga taccttttcc catcacgcca gaagaaaaaa cttggtctgg
    33601 tacttcactc aatggcagta ctttacccgt taaaggactt tttaaggtat gccgtgctga
    33661 agtaatcggt tttcctgcat tcgcatgttt gttttctaat tgattgtctt gtgtttgttc
    33721 gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca aaagcaattc ctgtagctat
    33781 tactgctact gtcacatttg actctacacc atccaccgta ttaatgaaag tcggaatcgt
    33841 caataagctg accaaactgc tggagaaagc ttttactccg gaaaatccga taatagctcc
    33901 accaatacct ccagaaatac aagcggcaat gaatggtttc ttcaaaggta atgtcacacc
    33961 atacacagtt ggttcagtga ttccaaataa cgaagtcact gtagaagaaa tccctaatgc
    34021 tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa gcagccccac cttgtgccaa
    34081 gatggctgga agaagcattg gcatcaatac atcaaaccca tattgttcga tattcaaaaa
    34141 catgatcggt acgaatcccc aatgcatgcc gaacatcacg aatacttgcc ataaacttcc
    34201 catgattaga cctgctacga ttggactaaa tccataaatc gagttgaaga gaccgcctaa
    34261 agcattaccg gcaaccgtcc cgattggtcc taaagcaaga aatgttaatg gaaccatcac
    34321 tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat tgcggaatca ctttttttac
    34381 gaatggctct aatttacttt gaacccatac ggctagaatg attggtatga cagaagaagt
    34441 ataaccagat ggtgaaagaa tcactgggat cccaaagaaa ctgatagctt ctcccgcacc
    34501 agcaactgct gtgatagctg gatacacaag tgccatagca attgctactg ctaaaaactg
    34561 gttggctttg aactttttag ctgctgtaaa agcaagcatg acaggtaaga aggtaaagac
    34621 accatctgct gcagcaaaca atatttgata agtaccgcta tcagctgtca gccagcccat
    34681 caccgttgcc aatgataaga atcctttcaa taccccggcg gctgccatcg cccctagaaa
    34741 aggtgtaaat atactagaga taatatcgat cagacgattc aaaatatttt tggactcttc
    34801 ttttccatcc tccgaaactg caagaccttc ctcatccacg atcgattgat aaacatctgc
    34861 tacattactg ccaatcacta cttgatactg accgccactt tgtacgactt ggatcacgtc
    34921 cgggtcttct tttaactttt gcgtatctgc taaagattca tcttttaatc tgaatcgcaa
    34981 ccgcgtagca caatgtacta aacttttgac attcttttgt cctcctaccg cttcccatac
    35041 acgacgacca actgcttgat tatccattgt tttttccttc tttctttttt atttaaaaaa
    35101 taaaaaccca gattgaaaaa aacagtcaca aacattcgtg aacctatttt ttcaatccag
    35161 gttttgcctg ccatacagta acaatcctta tgattggatg ctttttttct cacttactac
    35221 tctttgaata tgaatcgtta aataaatctg ctctgcgatc cccatttcct gtttttgcgt
    35281 ttgtcgaaga tactcattga tccgttcgac acaatgaaac gcttttggat actttccttg
    35341 aactaatgcg tagagaaatt catcttgctc gtcagatcct tcccctttga gataacgctg
    35401 ggcaaaaaac tgcagatgag taatgatacg ctgataattc aaagattctt catcgaacac
    35461 gatgccgaag aatttactga tgatactcag tatatctctt accattgtgg tagcggtcat
    35521 cgtcacttgc atgtcttcgt ttccttgttg gctattcaca aaatgaaagg caataaaacc
    35581 ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc acgaacaaaa gagcctcttt
    35641 cgctgcttga tattctttcg gataaaattt ttttgtttca aataataatg gattaggcag
    35701 ttggatattt tcttttgctc gtgtgatcgc ataatgaaga tgatcggtca atgtcagata
    35761 aatattaggc gaaaattcaa cttttagttt atcttcagcc agttcgacta cttgtttgac
    35821 ccattcgatc tcctctgccg gaatatcagc catcagctgc tggaattggt ttttagcaaa
    35881 agaatctctt aaaacaaatt ctttttggac cgcttctggc gaaatctcgt ccccaatctt
    35941 tttttgaaaa gctagtcctt ttcccataca aacgacttct tccccgaatc catttttcga
    36001 aatgacgaca ttgttattca aaattttatc aattttcatt ttgcttcgcc ctctcaagaa
    36061 aaataaaaaa acctagaatt cgcctactgt ttcttagctg aattctggtt ttgcctgccg
    36121 tacagtaaca atccatttct tattcaaaat catcatacca tcgaattttt atttatgcaa
    36181 gccttttcat ataaaaaata aaaaaactgg aacacaagtc tatctagaaa acaaccaaat
    36241 cagagatgca cctctttgcg tttctctttt tttcagcaaa ttggtttttc cttatcgatt
    36301 catatccttt ggctgttttt aatctgtaac gacttatgtc ccagtatcct aaaatgtttt
    36361 tgtctctaga ttgaaagcag atctcatatc atctgtcgca tcttttatcg atgtaacact
    36421 ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc atgtcgccac aagtaattag
    36481 tgtgacaatt tcttttccct cgacttcatt cagtaattgg gtatctgtag gagcaacttt
    36541 ttctttaaaa aatgttttgt atgtatacac attttctaaa tctgtcaaat agatcttggc
    36601 acccatctct aaattttcta gcggtgtaaa cagcaattca gggttcgttg cacgatggct
    36661 tgctaaggca taatttcctt ttcccatttc ttgatctgga gataatgttc ctgctccgta
    36721 aagtagtgac gtattatcca agcctttgaa aaccggtaaa ttgattgaaa ctgaagggac
    36781 tgcgatacta gcaatcactg gtagatctgt tcctctcatt tgcgacctca tgaccccttc
    36841 tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca tctttatcgt tattcttttt
    36901 cagatcttct tttgttacat tcgctatagc atatttatct cctgtctctc tgacaaaata
    36961 atccttgatc tgttcattga aaatcaaagc tagtcctact aataacaaga ggaacaagag
    37021 gatattgact agccatcttt ttgcttttcc tttttttttc attttctctc cctccagact
    37081 cattttactg tttttctttt gctctttcca acagccgaat gacacatttc tacaactctg
    37141 tgtatttttt tcgtacacct gcgctttttt ctacaggata tttcaaatta tttttgatca
    37201 atttttcttc aatgatctca tctagatcca ggtgaaggtt gtctgccatc atgtatgaat
    37261 agatcaatac atctgccagt tcttctttga ttcgttctaa ttgtgtttct tttacttctt
    37321 ccggcgtttt ccattgaaaa agttctaaca attcagatgc ttccaaagaa atagagatag
    37381 ccaaatcttt ttcgttatga aattgtcgcc aattacgttc atctctaaac tggttgactt
    37441 tttccatact attcatttgc ttgccttctt ccaatcactt cttacttgat taatcatacc
    37501 atttttttac ataaaaaata gatgatgctt ttaaaaaaac tcctgttttc ttttatttaa
    37561 aataaatgtc atagaaaatc aataatttag gtagccacca tgtgtatgca gtagcgtgtc
    37621 ttcttccctt gaggcttttt gtttttcgat acgttcttct tcccaaacag acaacgctac
    37681 tagaatctct cttaatgatt ttccttcttc ggtgagcgag tactctactt taggcgggac
    37741 ttggttatag gcttttcgat ggatactttc atcttgttct aactctctta actgctgggt
    37801 cagcattttt tttgtgatcg tagggatttg cttgcttctc cacgcctaaa ataagcgata
    37861 ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca ttttgacgat cgtgggttcc
    37921 gcttttcact tcttatctac ttagtatcat gaagtatact ttcgtactaa tactaagtag
    37981 cggaagtgtc aactactttg tttaactaca tagcaaaaga aacagaaggg tgacaacggt
    38041 catcacgctt ctgtttcttt tgaatatgct gtgttgttgt ctctatctcc gtgtagactt
    38101 tcctcaaact tatcctttga cagtaagtca gtaggctgat ttgctctttc tagatgccag
    38161 atacatttct acaaaaaaat agtgattctg ttttgaaaat tcgattttta gttctgcatt
    38221 ttcgtaaaca tccaacattt ttttcgcact gtatagacct aacccgccat tttttttaga
    38281 ggtttttcct cttttgacca tctccgatag cgtcaaattc gttggatttg ccttgttcgc
    38341 tatactaaaa cgtgatccct ctttttcttc cagcagattg atcgtaatcg gactctgatc
    38401 ggctacacga tgatcgaatg cgttagaaat aaaaatagat aaacagcgaa taaaaacaac
    38461 cagatcaatc gggattgttt tcacttcgac aggaataact acttggatcg ggattttctc
    38521 cgattctgct ttttctagaa attttaaaaa gacacccctg acagctgggg atacaatatt
    38581 tgttagctgt tgtatttgcg gtttttcaaa aatagctgca gaatgatctt ctaatatttc
    38641 taaaaattcc aacgtttctt ttttattttc ctgaagagta atttttaatg aaaacagtag
    38701 acttttataa tcatgtttga attcctctag atgatggtaa tattcttcat ttatttgata
    38761 cagttgaagc gattcctgat gttgtctttc ttttacttgg aaactgatca acataatgga
    38821 aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga gatagaagca gataattaga
    38881 tgagcggaag tgaacacttc ccaataccaa gtaaatcacg ccccagctta ataaaataca
    38941 aatacctcca aaatagtgtt cagttggata agtaaaaagc aagttgagaa atctcgtctg
    39001 tttgtctata ctttttacta atagatagaa aataaatgcg ataagtagtg caaaaaaact
    39061 tgctgttcca agatgtctct ttcctaaata atccatcaga tcaaaaacca gaaaaaagct
    39121 gagtccagtg atagcaaaaa aaagattact gatcaaacag ctgataaaca ggtttttcgt
    39181 agtaaaataa aaaagaaaca tcaataataa gccaactagg taaggaaagc tcactttagg
    39241 gataggaagt aaaagtgtca ccaccatcaa agctatacaa ccaaagctgt tttttactag
    39301 cagttgtttc gaaaagaata aatagatacc taaatctaaa atcaaagtaa aggtcaaata
    39361 aagtgtcact gttgcgacca tcttattcct ccattgtttt gtttcaaaat gtacacatat
    39421 ttttttaata ggagcctgca tatgaaaaag tattttatca tagaagataa ccagtttcac
    39481 agcgaatgga tcatcagaga attggtcaat catcatatcg agaatatcgt gcagctcact
    39541 tctatctctc aaatcaatca gttgatccag ttgggcacta ttgataatca cgatgttttc
    39601 tttatcgata tcgacttgaa gcaatcactc acaggtattg atgtcgccga acaaatacgt
    39661 aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt atacgaattt tgcagtagag
    39721 atcatcaatc ggaaaatcct tccgattgga tatatcagaa aagacaggaa tttatctgaa
    39781 caaattcgtc aagtgctaga acagatcaat tggaaagaac agcagatcgt aaatgtcgat
    39841 aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt atcctcggga aattttatat
    39901 attgaaagta tcaaaggttt gaaaaaccaa gttatggtca aaacaactac agaagataaa
    39961 ttagtgaata cttctttaaa acaattgaag acagagttag aaaagtatgc ttacttttta
    40021 cttttaaaat cttactgtat caacttatcg caattgcaga aaattgattc tgctcattat
    40081 gtgttagaat ttttcaacgg tgactcctta ctcgttggtc gaaagatctt tgaaaagaca
    40141 aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt gatgaataag agctgagacg
    40201 taagcatagc agatttcagg gagtgggcct tgaatcggct ccactccctg aaatcgttga
    40261 tatgatttta cgcttcctct tctgtaggag catctgttaa cgaccatgtg aatgttgaaa
    40321 cgtagttgtc tacagcataa ttggatacag gaacaaataa ttccactgat tgtctccctt
    40381 gttccacatt ctctcctaat gaataaatcc atgtgccaat cccttcattt gctcctgcag
    40441 ttactagatc tactgcctga tctgctggga tttcgattcc ttcagcgcca gttccagaaa
    40501 cggtagatgg cattccactg ctattagttg cgctatttcg aatcacggcg tttttgaatt
    40561 tcacctttga accagttagt ttattcgtat cagatgttgt accaataaat tctgttcgtt
    40621 tgactgataa tttccaaccg gcataatttc cgcttttatc cgttacttgt acgaaattct
    40681 gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg taatgcagca tatactgttt
    40741 ttgctgtacc taacttgatt gttccaaaat caagatctgg agcttggtca atcgtcagta
    40801 atccggatgt tccctgattt ggttcgtcag gtgtaaaagg agtatcagga tcatttggat
    40861 caacaggagg aactggaaca acgtcttcag gatttgcagc atctggtttg gtcacagaaa
    40921 tcgttccttc tgtagtggct gagctcttat tctcagcaaa aacactttgt gtacataata
    40981 atagacttcc agctagaacg gtacttaatg caattgtttt tttcatttct ttttccttct
    41041 ttcttttaaa ataaataatc cagcaaataa gccaataaga atgactccaa caagtatcag
    41101 ccatcgccag tcaaatgttt ctggctgatt ttttaaatca atatcctttt cattcaattg
    41161 gtccgctgca tcatcagtta tttcaaaatc ggtatggaga ttccagcgtt tttctttcca
    41221 agcacccttc acaaccgctt gataatgtcc cggagctaat ttttttcctt ctaatgggac
    41281 aagtaaatca aaatttgtat tgggtgctac atctattttt tcttttttta ttgaaaggta
    41341 aggctctttt gtttcttttt tgtagatttc aaccgatgta gtcaagccga acatataaga
    41401 agcatttgga ttttgaagat tcgccttaat cacatttctt ccatcgcttt gatccgcata
    41461 agcttttttt aatttcaaat ctggggaaga ctctttttcg ctatttcaaa tcaagattgc
    41521 tttcacttga gaaaactgat tgacgacacc aactgtactt ttgttagaaa cgttctcttt
    41581 tttttcttga aacgtgatcc ctcctgcgac cacaccagcc agctcttttt ctggcgcttt
    41641 cagtgaaagt gtctcagtga ccgcactttg aggaggaaca gttatttctc ccactgtttt
    41701 tgctaagttt ttcatatcat aacttaatgt ttcatcaaat ttactttttg attctccata
    41761 ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc aatgtctcaa ctgttactga
    41821 tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt gttttcccag gctccatcct
    41881 tacatttata tatgttttgt tcttatcaat ctgattagcc ggtaattcaa aatctaccgc
    41941 aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg atgaatccaa ggagaaggat
    42001 cataagtaaa gagcagttgt ttcgatgact tttattccat attcgattca tcaatcatcc
    42061 tccttgatct ttattctgtc ggcgcatctg ctaatgtcca tgttatctct gctgagtaat
    42121 taccagctat ctgattttct ttggttaaga caagtttaaa cgcccgaccg ttatttaaat
    42181 attctgaata atctgcttgc acattcgttg aattttgtcc tctgacaatt tccagttctt
    42241 cttcattcac taattgatac tcctctcctt tcttgaaata taggacatcc tgcatagaaa
    42301 atcctgcatc atttgtgaaa ggctgagata attttatcgt taagatccac ggatttttcg
    42361 tttctcgctg atcataaatc cataattttt cctgtatatc catttggcca aatgcttctt
    42421 gtgtcaaacc agttgctttg atctgaccaa aatcgagttt ggaaggggca agaaatgaga
    42481 gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg cggggcatca tgataaacaa
    42541 ctggcgtatc acttggattg tgattcccaa tttcatcgtt ggtacttggc ttgttctgca
    42601 cacccttagc agtagcttct cctgcttggt cgtttaatac aatttctaat acatctcctg
    42661 caaaaagttg attttctggg atagaaagaa caaattcccc tgtatcatca atagttgttt
    42721 ctgacaaaac agcttgattt tttttcaatg aaaccaaggc tcctgcttca cctgtacctg
    42781 tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa attagcaggt tcaggaggaa
    42841 tcacatcgac tgtcgttttg gcagagtcca gttctgtcac ttcttggcca cctcttactt
    42901 ttttagcagc aacaacttgc acttgatcct ccgtttttaa tctctcatca ttgggtactt
    42961 gaacttcgaa caagccgcct tgttcgcctt ctccccatgg gttatatcct gccccttcat
    43021 tttcatctgt ttgtccttgc gtgagagcag taatttctac agatgatttc gtcgtatctg
    43081 cgtaagtaat acgtaaagtc acttctactt cgcctgtcca agcatctctt ggctttgcct
    43141 cgacaccttc gggaacaacg acatgcccat agattttttt atctgcattc gttggcactc
    43201 ttaaggattc aatcatcggg agctgattat tggcactgat tcgattataa tttttcattc
    43261 cggcacctgt ctgagcaaat tcatttgtta aacctgcact agggcttgat acgaaattgc
    43321 ctaagtcggt cccattgatc gataagtctg tcatatcaga aaaataatcc gggtcttttt
    43381 caaagttatc gaatgttgcg cctgtttttc gccataaagc gatttctgaa tgggcgatct
    43441 ttaaagaaga atctccattt ccttggaata cccagccgcc tatgccattg gtctcgcttc
    43501 caatccgttt gttttggaaa tcaaaatagg taggagattc taagtccaca ttcaacacat
    43561 ccccactgat gatgccggtc ttatcttccc ctgttttccc gaccatgact aaataagttt
    43621 tttcacctgc cgtaaaattt aaatcaatat ctccagttgt atctacagtt gctccggaat
    43681 ctgcgcgtat atcgatattt gatttttctc cagtgactgt aaaatcagct gaacctggac
    43741 tttctagaaa accagaagcg tcataaaatt ggatcgcttg atttccttga ttgagtctgt
    43801 tcacgtaaga tttgccatca cctgtatttc ttaccctcaa tgagcctcca ttggttactg
    43861 tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga acgcccactc tgcttatcga
    43921 tgtcgataac tgatccggct ccttctacga tcaaagaaag gccttttcct acaaaacgca
    43981 tcccattgtt cccgggttga tcgtcatctc cagccatttt gaccgagatt tgtccaccac
    44041 ttgatacatg gatttgacta gaatctcctt gcaagcgaaa tccgcccgta gttgctgtat
    44101 tgatggtgat tttcccacca ttcgatacag tgactttcga tcctgaacct ctcatttgga
    44161 tgattccgcg attgttgtca ttggtaatat tgatctttgt atttaccgaa aattcgcttc
    44221 ccgaaccact gacttcaaac atgacattac caggtagcgt atcgactgcc tctccttcat
    44281 cgctaacgcc atctgcccga tcataatcaa gagagacctt tgaaccgctt aagatatgga
    44341 ttcctggcgc tgtttttcca ctaccagtct catctcctga atctagaaaa ccgtagaatt
    44401 tccctagata cggtccataa agttgactac cattgatcac attcaattga tagcttctac
    44461 aaaaataggc tttacccgga gcggcattca gtccttcctt cactggttgg acatccaaca
    44521 caccggaaac accatctaat gtgacagttg cacgaggagc gtaaatcagt cctaatttgg
    44581 acccatcata caaatcaagt gtcccggtaa attttatttc tcccgtaaaa tgatatctgc
    44641 ttccccaaaa atcggtcata taagcaatga taatggcatt tccatcgata tttggtgtca
    44701 atccacctgg atgtgcagct gttcctttaa aattctggaa ctcgatcgac tgaggtatct
    44761 cattgatata aatatggtgt gtgccgaaat tgtgagcgaa tccaccaaaa tcaataacga
    44821 tatctcgttt gatctctaat tccctgtcta aagtcaaagg ctgaatcagt cggatcttag
    44881 ttactgattc gtctgcgaga agctgcgcca gttcttctgt tgtggcagct tcgctgtacc
    44941 catccgctga aacagctctt gccagtgccg cttcactgct atcagacgtt tgttcagcta
    45001 cttctgaggt ctcacttgtc ttattttctt cttgcctttt attagtagaa gcctcacttt
    45061 gagattctgt agtttcagaa gtaatcgatt cttctgaaaa agtagtcttg tcaaaacttt
    45121 gattctctga ttgcgttgat tcgtttgttt tctctacatc ggcaaaaacg gatgtcacca
    45181 tcaagagagg gaagacaaac aaaatcgacg caatcagctt ttttacacat tgattcattc
    45241 tccaccatcc tctctgacgt tttgacaatt ttattttatc gttttaatct atttccaatt
    45301 gtactttcat atttaaaata tgtattttta tggaaaaatt tttctattag gtgtaaattt
    45361 cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc acagtccctt atcccgaaca
    45421 agcggtgttc tcccgccaac tcctcggtca caacctccat tttaacaata aacctgtaaa
    45481 tgtgagggca atactttgat tgtcacaggt aatttatccc cttcatctcc atcaacattc
    45541 gtcgtcaatt cttcctcatc agctaatgac acaactaatt gctcacaagt aaggtaggct
    45601 agatttttcg tagattcatc tactcctttt aataggtctg gcaatgtttt aagcgtatcg
    45661 atcaaagaag aatcttttaa ataaaccaaa tggagcttac catcatccac ttttgcattt
    45721 ggaagtaatg tttcaaaacc gccgactgag ttagttagtc ctatcagcaa ggtactgctc
    45781 tcaatttctt ccttctttcc atcaacttct acgtgaaacg agtaggattg tgtggaagct
    45841 aactgtttga ttcctgacat gaaataagct agttttccaa atttcgtctt cttctccgca
    45901 tctacatcat tgattgattc agggatagag ccaattgcga cgacattcat aaaatagtcg
    45961 tcatttattt ttccaatatc caatggcttg actgattcaa tcgaaaaatg attgatcgct
    46021 tcctcaggtt ctagcggtat ccctaatgct cgagccaagt cattcaccgt acccaatggg
    46081 aaaaatccga aatttggccg atgttcttgt tcagcgattc cactgattcc ttcgttgact
    46141 gttccatctc cgcccatgac aaacacacta tgataccctt ccgtagccgc ttcgcgagta
    46201 aagttttttg catcccctgc tttttttgta tgcaagacga ccacttcatc aaacaccgat
    46261 tccaattttg ctatcgctaa ttgctcaaat tcctttgctt gttcaccgcc tgaactgggg
    46321 tttaccacta ataaaacttt tttcatcact ttctttccct tctgattgat ttctttaata
    46381 taacgatagc ataaagaaaa aaattaggct gtgatgaagt actgttcatg aaacattcat
    46441 ttattttttg acaagataaa acatccatac tatgaaactg aggaaaatta aaacaatgat
    46501 ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa tcttaggtgg
    46561 cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact ttttgttatc
    46621 agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt gatgtactgt
    46681 taatatgtca cggccttgaa taataaatgc gacaatcccc gctataatta gtgtgcctaa
    46741 caccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg tacttcctgc
    46801 ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa ataaaagaaa
    46861 tttgcaaaac cactccattt atctacttct acgtgcattt tttgatattg ttttctttat
    46921 ttaagattat acagaaaaat atctagggtc gctggattct aagtaaaaaa aatttttaaa
    46981 ataaaaagaa ctaaatcaag tagtaattaa actactgttg atttagttc
//
LOCUS       DACDSR010000013        45258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDSR010000013 DACDSR010000000
VERSION     DACDSR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.013"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(215..1606)
                     /locus_tag="IWK15_000755"
     CDS             complement(215..1606)
                     /locus_tag="IWK15_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9546378.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1819..2205)
                     /locus_tag="IWK15_000756"
     CDS             complement(1819..2205)
                     /locus_tag="IWK15_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9546379.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2261..2884)
                     /locus_tag="IWK15_000757"
     CDS             complement(2261..2884)
                     /locus_tag="IWK15_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9546380.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2948..3616)
                     /locus_tag="IWK15_000758"
     CDS             complement(2948..3616)
                     /locus_tag="IWK15_000758"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9546381.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3628..4068)
                     /locus_tag="IWK15_000759"
     CDS             complement(3628..4068)
                     /locus_tag="IWK15_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546382.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4127..4799)
                     /locus_tag="IWK15_000760"
                     /pseudo
     CDS             complement(4127..4799)
                     /locus_tag="IWK15_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4984..5382)
                     /locus_tag="IWK15_000761"
     CDS             complement(4984..5382)
                     /locus_tag="IWK15_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9546383.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5379..6152)
                     /locus_tag="IWK15_000762"
     CDS             complement(5379..6152)
                     /locus_tag="IWK15_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9546384.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6149..6832)
                     /locus_tag="IWK15_000763"
     CDS             complement(6149..6832)
                     /locus_tag="IWK15_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9546385.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6999..7886)
                     /locus_tag="IWK15_000764"
     CDS             complement(6999..7886)
                     /locus_tag="IWK15_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9546386.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7883..8752)
                     /locus_tag="IWK15_000765"
     CDS             complement(7883..8752)
                     /locus_tag="IWK15_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546387.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8889..10175)
                     /locus_tag="IWK15_000766"
     CDS             complement(8889..10175)
                     /locus_tag="IWK15_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9546388.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10448..11026
                     /locus_tag="IWK15_000767"
     CDS             10448..11026
                     /locus_tag="IWK15_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9546389.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11026..11955
                     /locus_tag="IWK15_000768"
     CDS             11026..11955
                     /locus_tag="IWK15_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9546390.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11952..13688
                     /locus_tag="IWK15_000769"
     CDS             11952..13688
                     /locus_tag="IWK15_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9546391.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13751..14587)
                     /gene="pheA"
                     /locus_tag="IWK15_000770"
     CDS             complement(13751..14587)
                     /gene="pheA"
                     /locus_tag="IWK15_000770"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9546392.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14584..15093)
                     /locus_tag="IWK15_000771"
     CDS             complement(14584..15093)
                     /locus_tag="IWK15_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9546393.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15086..16381)
                     /gene="aroA"
                     /locus_tag="IWK15_000772"
     CDS             complement(15086..16381)
                     /gene="aroA"
                     /locus_tag="IWK15_000772"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9546394.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16394..17485)
                     /locus_tag="IWK15_000773"
     CDS             complement(16394..17485)
                     /locus_tag="IWK15_000773"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9546395.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17502..18668)
                     /gene="aroC"
                     /locus_tag="IWK15_000774"
     CDS             complement(17502..18668)
                     /gene="aroC"
                     /locus_tag="IWK15_000774"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9546396.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18670..19734)
                     /gene="aroB"
                     /locus_tag="IWK15_000775"
     CDS             complement(18670..19734)
                     /gene="aroB"
                     /locus_tag="IWK15_000775"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9546397.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19767..20789)
                     /gene="aroF"
                     /locus_tag="IWK15_000776"
     CDS             complement(19767..20789)
                     /gene="aroF"
                     /locus_tag="IWK15_000776"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9546398.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21448..22422)
                     /locus_tag="IWK15_000777"
     CDS             complement(21448..22422)
                     /locus_tag="IWK15_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9546399.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22403..24286)
                     /locus_tag="IWK15_000778"
     CDS             complement(22403..24286)
                     /locus_tag="IWK15_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9546400.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24304..26529)
                     /locus_tag="IWK15_000779"
     CDS             complement(24304..26529)
                     /locus_tag="IWK15_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9546401.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26644..27678
                     /locus_tag="IWK15_000780"
     CDS             26644..27678
                     /locus_tag="IWK15_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9546402.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27745..28758)
                     /locus_tag="IWK15_000781"
     CDS             complement(27745..28758)
                     /locus_tag="IWK15_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9546403.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28836..29024)
                     /locus_tag="IWK15_000782"
     CDS             complement(28836..29024)
                     /locus_tag="IWK15_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9546404.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29464..29592)
                     /locus_tag="IWK15_000783"
     CDS             complement(29464..29592)
                     /locus_tag="IWK15_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9546405.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29595..30968)
                     /gene="rlmD"
                     /locus_tag="IWK15_000784"
     CDS             complement(29595..30968)
                     /gene="rlmD"
                     /locus_tag="IWK15_000784"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9546406.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31079..32134)
                     /locus_tag="IWK15_000785"
     CDS             complement(31079..32134)
                     /locus_tag="IWK15_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9546407.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32144..33574)
                     /gene="gatB"
                     /locus_tag="IWK15_000786"
     CDS             complement(32144..33574)
                     /gene="gatB"
                     /locus_tag="IWK15_000786"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9546408.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33571..35043)
                     /gene="gatA"
                     /locus_tag="IWK15_000787"
     CDS             complement(33571..35043)
                     /gene="gatA"
                     /locus_tag="IWK15_000787"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9546409.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35043..35348)
                     /gene="gatC"
                     /locus_tag="IWK15_000788"
     CDS             complement(35043..35348)
                     /gene="gatC"
                     /locus_tag="IWK15_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9546410.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35454..37490)
                     /gene="ligA"
                     /locus_tag="IWK15_000789"
     CDS             complement(35454..37490)
                     /gene="ligA"
                     /locus_tag="IWK15_000789"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9546411.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37507..39741)
                     /gene="pcrA"
                     /locus_tag="IWK15_000790"
     CDS             complement(37507..39741)
                     /gene="pcrA"
                     /locus_tag="IWK15_000790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9546412.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39896..41455
                     /locus_tag="IWK15_000791"
     CDS             39896..41455
                     /locus_tag="IWK15_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9546413.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41566..43497)
                     /locus_tag="IWK15_000792"
     CDS             complement(41566..43497)
                     /locus_tag="IWK15_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9546414.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43515..44429)
                     /gene="pfkB"
                     /locus_tag="IWK15_000793"
     CDS             complement(43515..44429)
                     /gene="pfkB"
                     /locus_tag="IWK15_000793"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9546415.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44426..45178)
                     /locus_tag="IWK15_000794"
     CDS             complement(44426..45178)
                     /locus_tag="IWK15_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9546416.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT        12539 a         9846 c         8096 g        14777 t
ORIGIN      
        1 aagctcaacc aaacgtaaaa tatgaggaac tatttttgtt tggctgttct tattacttga
       61 tgcagggcag ctttttcaca acctcttatt cacggtgttt cagaagtagc ttctcggaaa
      121 taagccgaaa tttcacaaaa atttgagaaa ctatttttag gaaacttctt cttatttctt
      181 aaagctgacc acttctatca caacctcttt ttgactatgc attcacatcg atcactttag
      241 aatcatcatt gatgacatcg cgatctaaat ctttgatgcg tgcacttgtc acgataccag
      301 ctaccatgct accgctgacg tttactgctg tacgtgccat atcgattaat ggttcaacag
      361 agataactaa cccaacgatt gctactggca agttcattgc ccctaaaacg atcagtgaag
      421 caaatgttgc gcccccaccg actccagcta caccaaaaga actgattgtt acaattgcca
      481 caagcatcaa aataaattcc aaactaaaga cattcactcc agctgctggt gcgacgatcg
      541 ttgccaacat agctggataa acgccagcac agccgttttg tccaatagac aaaccaaaac
      601 ttgctgagaa attagctgtt gcatcatcta ctcctaaagc ttttgtctga gtctcgatat
      661 tcaatggcaa agctccagca cttgaacgtg aagtaaacgc aaagctcaat accggaaagg
      721 cttttttgaa atagttgatt ggattgactt ttgcaccaat caaaatcagt gagtggacaa
      781 tcaaaacagc aagcaatgct acgtaagaag caacgacaaa tttacctaaa ttgacgatag
      841 ctgcaaaatc acttgttgcc aaaacgtttg tcatcaaagc aaatactcca taaggtgtca
      901 aacgcaaaac aagcgtcacg atccgcataa tgattttata cagactgtcg actaaatctg
      961 aaaagaatgc tgcttctttt ggcgcttttc ttttcacgcc tagataagcg attccgacaa
     1021 atgccgcgaa aataacgact ccaatcgtac ttgttgcacg actacctgct aaatcagcaa
     1081 aaacgttttt agggataaag gataaaatct gctgtggaat cgataagttt tcaactgttt
     1141 cctgacgtgt tgccagttca gagattcgtg ctgtttctgc agctccttgt gtgaatttag
     1201 ctccttggag tccgaacagc attgttgaac caattccgat caatgctgca atagctgttg
     1261 ttccaagtaa agaagtcaat acagtcgcac taattttccc tagcttttca gaaactttca
     1321 tttttgtgaa tgcaccaatg atcgaaataa acactaaagg cataactagc atttgcaaga
     1381 atgatacata tccttgacct acaatcccga tccattccat tgattgagta acgactttgt
     1441 cgtctacacc aaagatcaat tgaagcagac cgccaaagac gataccaact cctaatgctg
     1501 taaagacgcg tgttgaaaat tttgtatggc gcttttgcat acgatagaac acatataata
     1561 atgccgcaaa aactagaacc actaaaactg tgatgagtgt tgtcattttt ctcctcctca
     1621 tattattaat gacaagtcca tcgaaggaag taaaggttcc cgagaatgat tcttcaacca
     1681 tcctcctgaa aaaaattttt tcagtgactt ttatatttta gacgattttt atcgaaatga
     1741 cattcttttt gcttacaatt agtaataaaa aaacatacct gtgatagctt taatctatcg
     1801 caggtatgtt tttactgtct attcttgttt ttctgattga aaattcaatt ttaccccagg
     1861 ctgatctaag gcgatctctg tcacctcata agacattcgg cgtacgatat cgaataacgg
     1921 tgctactagc tcgtccactt cttcttgtgt gacatcattt tgaccttcaa ttttccgatt
     1981 gaggatatga ttgatctgac tcacacttcc agataaaaca tatgtatcaa acactaaacg
     2041 gaactctaag cgtgctccta cgatgctatg ttctttcgga tagttttcga tcgtttcttt
     2101 taaaggcgag aagcctactt tcaaatctgt ttctacttct tggttcggca atctcatatc
     2161 ataatgaaat gcttctacaa cttctttttg tcttctgatt tccatataat tttatcactc
     2221 ctttatgaaa attataacat atctcctttg aaaggggata ctacttttta atgatgctct
     2281 gcaaggtaag gatttcattc agatttccta ccacataatc tgccgggatc tcacccagaa
     2341 aatgatcgat gtcgtaaaga caagtagcga ctcctgcatt tttccctgct tcgatatcta
     2401 aacgacgatc gccgatcatc atcgtgtctg ttctttctaa atgaaaagta tcgatcaaat
     2461 aatttaaaga tgctggatca ggtttacgag gaaagtcctg atcgatccct actacttcct
     2521 caatcagatg tgccaatcga tgttctttta acaggcccca tgttgattct gtcaaacggt
     2581 gggtcaggat gaaatgacga ccgccgttgt cttttagcat ttccagtact tgtttcgttt
     2641 ctggaaatgg acgggaaact tccgtacttt ctttttcata tcggtgaaat aattcattaa
     2701 actctctggc attcaagttt gattcattga tcaaataact agtagaatac tttttcattt
     2761 tgaagtagat ttcttttttg tccatactga taccaaaatc ttttagtgct tgccatgcac
     2821 catctaccat tgcgggatac gtatcaaaca atgtaccatc aaagtcccat atataatttt
     2881 tcatgttcct tttgttccat tcctttgctt cttttttcta tctctcactt ttagataaag
     2941 gtgttcttta aagtattttt ttgtccagaa ttgtcaaatc atctgctaat gtatactgga
     3001 ttggttgtgc atttgggata tcgatcgtat ccatttgatc ttcaggaaca tcttctaaat
     3061 attttgtcag tgctcggatg ctattaccat gacccacaac tagtaaatct tgccgatctt
     3121 ttagttttgg tgctagatga tcttgccata agggaatgat ccgattcact gtcatttcca
     3181 gactttctcc gtatgggatc aaatgcggat ccaagtgatc atatctgcgg tcaaatctat
     3241 tttctgcggt taacggcgga acttccgtat atcctctgcg ccatgttttt acttgatctt
     3301 ttccgtatat ttctttcatc ttatctttgt ttttacccac aagatcccca taatgccgtt
     3361 catttagacg ccatgttttt attataggaa gccataactg acccatttct tctagtgcga
     3421 cttgtccggt aagaatcgca cgtttcaaaa cagaggtata aacggcatcg aattgaagtc
     3481 cggcttcaag cattttttga ccggcaactg ctgcttgttc ttttcctttt tctgttaagg
     3541 cgacatctag ccatcctgtc cagtaatttt caaaatttgc ttcgctttcg ccatgacgta
     3601 ataatactaa tttcattctt ttctccctta aacattcgtt tcgaatttta taccacatgt
     3661 tttgcatgtc acacgtattc gtccttttcc tttaggtgca cgctgctttt gatgacaatt
     3721 cgggcactca aaaaaagtat aactttttcg atccattttg acttgctttt tgttttttat
     3781 ctgatgatat ttttctttga ctctatcaag atatttcaaa taacgttgat tctcatttag
     3841 tctggggtag attttttttg ataggaaacg aaatatcatc catccgaaaa agacgatgaa
     3901 caacaattgt ccgattctat agggcaacca acgacttagc aaactaaaga caagcgcagc
     3961 gatcaataga ccgcgattca actgatcaaa tttcgcatat cttccctgca taagtttgtg
     4021 atagttctgc tgaaatcgta ttagcctttc taaccatgtt tgcatcatat tatctcctcc
     4081 atcatctaat atttattatg acaaaaaaat ccaaaaagaa aaagccttaa ttggcctttt
     4141 ccattgcaac ttggtgaagg acattccgat ttctggtaaa gtcaatcaca atcactttct
     4201 ttttatcttc tctgttgact actcgaaatg tgtccgtcaa tggaagaaac agcttcttag
     4261 atggtagctg ttgcatacgt ataaaacgat ggatcatcgt aatcaattga ggaaattgaa
     4321 tagacgtgtg aagcagacct ggatattttt tcaacagctg tgtcgttttg atcagattcc
     4381 gtttttttac tgatcacttt taaaaagctt tttagaatct tttgctgacg tctaaaaata
     4441 gaaaaagaat tttcgtcatc tatttgataa cgcatttgtt tttgcagttc tgctgcagtc
     4501 agatggatct tcccatcgtt tgtatcgatc attaaacctt ctggaaacag ttcaaccact
     4561 gctcgatccc aagattcaag agaaagctcg atataatctt ttactggtaa ttccaattct
     4621 accttaaaaa agtgcaaaac agccgacagc ccttctgctt gatagatttc tcctaatgtt
     4681 agcccctcta gatggaactc tggtgaaaaa tgcagcaatg ttttattttc ctgcaattga
     4741 acatatagaa atgtagatcg ctcaatcaga gtatctttta aaacaactaa aaaattcatt
     4801 tttctccccc ttttttcatc atttttaaaa tagcatattt tgaaaagaaa gtctgatgct
     4861 tagagtagaa tttcataaaa atttactatt tcataagtat tcacgagaaa aatacaaaca
     4921 aaaaagcgga aataacgctc atccgccttc ttgtttgtgt ttatttcctc ttcaaacgct
     4981 tgtctatgat tcagactttt ctctatcgtt attttcatct aaaagttctt gcattccgat
     5041 aatttctcga tattgatgaa tcttagtgat accaatgact ttgtctacat caatactaaa
     5101 acaaatacca ttacctggaa tatcgatgtt gatgccttga ccaatcgcat ccattacttt
     5161 attcgtcaaa ctatcgggta ctaatgtcaa cacaatatct ttttctggcg aaatgtccaa
     5221 acctaaaaat ttccctgaat catgtacacc agaacctctt ccatgtaaga tcgttccgcc
     5281 tgctgcacct gcttcttttg aaaagtcgat aacatcttcg cccattcctc cgtcaacgat
     5341 cgtcacaatc atgtgaagat tcaatgctac aagatctttc atttgctttc ccccaatttt
     5401 tgcaatcttt ctttttcgat tctgtctttt tcatcttttc gtttataaat atatcctagt
     5461 actaaagtgg ataatattgg tgtcaatgca atcattgcaa ccacaccaaa cccgtcaatg
     5521 atcggatctc ttccttcaat ttgtgcagcg attgccacgc tcattgataa aataaatgtc
     5581 gaacacatcg ggcccgttgc tactccaccg ttatcaaaag ccattgctaa aaagatcttg
     5641 tccacttttc gtcctaaaat aaatgctagc ccgtatcctg ggatcaagaa ataataaaga
     5701 gagaatccga cgagcaaacg ccacatagac aatcctactg ctgcgccgat cccaattgaa
     5761 attaccatca gcatgatttt cgatcggatt ctcccctcgc tcaattcttc aacctgtttg
     5821 atcattacat gaatcgccgg ctcagcaaac cctactaaga accccattaa aaagccaagg
     5881 ggaaccagta tataattgta aggcaatccc gcaatagcac tgcctaaatg ctgtcctacc
     5941 ggtacataag ccatattgac tccgtgcagg aaaaggatca acccaatagt cgttatcaag
     6001 aaacctttca taatgtcaaa gaaacgcttt ttgcgcatac gaaaactaat gatattcatt
     6061 aaaacaaata ggattacgat aggtgccact gccaaaatga cttccatggt aacgatttcg
     6121 attccatcta tcacttgctg ccagaaactc attggaagat cacccccaaa atcatcacgg
     6181 caagaatcgg ccctaacgaa gcaataccga ccattccaaa actgtcattt tcatttttct
     6241 tttgtttgat catactagtc atccctccgg caagggccaa gataaatgga acagttactg
     6301 gaccagtagt cactcccccc gaatcaaaag caatcggcat aaattcggaa ctggtgaaaa
     6361 atgaggcgat caaaacgcct atgtagccta tagccatcaa ttgataatag gatagcttga
     6421 atacgacccg aagcagcgca aaggctagaa atactccagt gccgacgctg acgatcccaa
     6481 tcagcaacac tctgccaatc tctccgtcag atatatcata tacttgttgt cccaaaactt
     6541 gcacggaagg ttcagcaatc gtgatcacga tcccgatagc aaatcccaaa ctgaccaaaa
     6601 cggtcaaact tttctttttg atcatatatt tccctactag atctccgact tccatcatag
     6661 aataatctgc tccaaacaaa aagagcgtca ttccgatcat catgattgcc gcaccaacca
     6721 gaaatgaagc gaggtcttct ccttctaacg gtgcaaaaac aaacgttaaa atgacaatca
     6781 ggacagtcat cggcaatata gcagcaatca cttctttaaa attttttagc atgtatcctc
     6841 ctcctttact ctttaagttt accttaaaat gaaagagttt tcgagcacaa acttaaaacg
     6901 ttatcattta atttttctca acataaaata acacacgtcc caaaagtatc tttttacttg
     6961 gaaaaatgac tttgaaggaa cgcgcttatt gtgttttttt attttacaga gccgatcatt
     7021 ccttgaacga attgcttttg caaaacaaaa aatacgatgg cagttggtaa agtagcgatg
     7081 actaatccag ccatgatcat tccatagtct ggtgtatagg aagcacccat agcagataaa
     7141 acaagcggaa ccgtccgttt atcaggagat tgcaaagcta ctaagggcca taaataattg
     7201 ttccagctac tcatgaatgt gatgattgca gccgcggcat aggtattttt agccgtcggc
     7261 atatacactc taaaaaaaat acttagttct tttagaccgt ctaatcgagc ggcttctact
     7321 agatcttttg gaaaagattt aacattttgt cgaaagaaga agatcaaaaa agcagtgcta
     7381 acagatggca ggatgacaac caagaaactg ttgattccca aaaccgttcc gttcaattgt
     7441 gaaaacattc gatataaagg gatcatcaat gccgcaaaag gaaccatcat cgatagcagt
     7501 agaatattga aaattaggtc ttttcgttta ctttgataaa tctcaaatcc atatcctgcc
     7561 attgacgaaa tcagcaatgc aaacacagtg gtcaccaaag cgatcatcgc tgaattccaa
     7621 agggaacgag taaaattcag ctcattggaa aacaaatttt gaaaattgat gatcaagtta
     7681 ctgccaatct ttatttttcc cgatgtgata tccacgggtg tattcgtcat tcccaaaacc
     7741 atccagacaa aagggaaaat ggaaataaat gataggatgc ttaatgctgt atactttaat
     7801 actgacccaa ttgtcgaacg aagttctaat gcttgttttt tgacgttggg gcgttcgatt
     7861 gtttcttttt ctaccatatt tttcatcgac tttctcctcc tactttcgtt tggattatcg
     7921 acaatatcac aataaaggaa acaataacaa aggaaactgc tgccgcatat ccaaaattag
     7981 gcgtgaattt ataactcaaa ttatagatgt attgagatag ggtggtcgtt gcatttgctg
     8041 gtcccccgcc agtaatattt tgtacttcat caaaaagctg caatgtcccg atcgtcgaag
     8101 taatggaagt aaacaaaatg attggtttta aatttgggat cgtcactttg ataaactgct
     8161 gccatttaga agcaccgtca atagatgctg cttcataaat ctccgaatca atattttgca
     8221 tgcctgaaag gaaaaaaatc atattgtatc cagtccaacg ccaagtgatc gaaagaatga
     8281 tcagaactct cgcccaaatt ggatgagtca accagccaat cggtgtgtca atcataccaa
     8341 tgcccaacaa caagttgttt acaaggccag attgagaaaa tagacttttc atgatcagag
     8401 aataactgac catcgaagtg atacatggaa gaaagatagc ggttcgaaac aaccctttat
     8461 atttcaactt tttatcattt aaaaggttgg aaataaaaag tgctagaaac aacatgatcg
     8521 gtacttgtac aagtaaatac aggaatgtat taaaaaaagc ttttttaaaa gttgcatctg
     8581 tcaacatccg ctgataatta cctaacccgt taaatgtcat attgacaggg ggaccacttt
     8641 gaaaagacgt gatcaaagcc aaaaccatcg gaataaaaac catgattgat atcaaaatga
     8701 ctggcaagat taaaaaagca tgcccgtttt tgaataaatc tttctgcttc attcttttcc
     8761 ctccaattct atcattgatt atctacatga aaagacaaga aagacaatat ctgtaaggca
     8821 gacgtgatct tttttgtctt ttcgtttgtc tgatgaaaaa gcgaagaaat tcactttttt
     8881 accaccgatc aattagccaa ttgtgcttct acttgttttt gtgtatctgc tagtaccttg
     8941 tctgtctctt ctccattgat gattcgatga agagcttcag ctacgactgc ttcaattgca
     9001 taggtctcat gtccgtaatt cacttctggt atttcagatg tccatgttga aaaatcgtca
     9061 aagatctttt gaccactgta aaattcagaa gcttcttgat aagcttcttg ttcattcgcg
     9121 ctaagcatcg tagatactag tccgatttct ttcgcaagag tgcccatcag ttcttcattc
     9181 gaagcaaacg ttgcttctag gaaatctttc gctgaatctt ctccagctac tcctttgatc
     9241 acataccagc cagcgccgcc aaggttagaa gcttgcgctt tttgcagatt ttctggaagt
     9301 gcaggtattg gtgcaatctt ccattttcca gattggtttt ctgctccttg gatcgttgaa
     9361 gaataccaag cgccagttgg gctagaagct actgctcctg attgtatcgc agttactcca
     9421 gcattccagt cagaggtttg ttcgactaga tcttctttca aaagagtggc aaacaattca
     9481 agaccatact ttaaagatgc attgtcttgg atcgtgactg tctccccatc ttcagatgta
     9541 taccattctc ctgcctgttg cataatcatc cttactcgac caaggtcaga aggattgact
     9601 tcagcgattt tctttcccgt cttttctttg acatctcgcg ctacttgaat atactcgtcc
     9661 cacttcagat tctccatatc ctcttctgta taacctgctt ctgccatcag atccgtacga
     9721 tagaagtttg ctgttacgcc cgaatcgaaa ggaacaccgt atattttatc tcctactttg
     9781 tttaccgcaa atttatacgg tgcaaaatga tcttccttta caatagcaga caaattagcg
     9841 aatgcatctg gataagaagt caaatagcct tgtatcctat aatcttcaat caagacgata
     9901 ttgggcaatc cttctgtatt cccgctcgct aaagcggtgt tcagtttctg aacgatatca
     9961 tcctgagaca tcgtaacaac ttctatctct gtttcttcgt tctcataaac tttttttgct
    10021 tcattcactg ctttgatatt gaatgtctca tcccacgccc agacagtgac tttttcttct
    10081 ttgtctgctg tactactagc acttccgcat cctgttaaga aacctgatgc tgccgctaaa
    10141 caaacgactc cagcaattaa ctttctgatg ttcattttta tccgctcctt ttccatccat
    10201 tagttgttta cgtttgctac attcatcata ccaaaatttg aaaacggttt ctttattatt
    10261 cagatacttc aaaaatttct cctaaaataa taaaaaaata caaatcaact atgacaaaag
    10321 gaataatctt agcatctaga ccaaagatcg aaatggtgag gtttctttaa aataaaaata
    10381 tccttgcatt ttctcctaga aataagtatt tttaaattac aagactattc ttaaggcggg
    10441 taaaaaaatg ccaatacgcg taattcttat cgatgatgaa cagacgattt tagatggaat
    10501 gaaacagttg atcaattggt ctgagcacgg gtttcaatta atagggactt tttccagtcc
    10561 gctgaaagcg cttgattttt ctttggtaca tgcaatcgat atcgtggtca cagatgtaat
    10621 gatgcctgaa ttaaatggaa tcgaattatc gcggctatta aaacagcaac aacctgaaac
    10681 acagatcctt atcctctcta gctatgatga atttgatatg gttaaagatt cttttaaaga
    10741 aggcgtcgct gattatcttt taaagcctcg attgagtcct gatttttttc taaattcctt
    10801 aactgccgtt tcacaaaaaa tcaaaaaaaa gcccaacaaa agcccaatca ccaatcgtta
    10861 tgagcagatc tgcgagaatc tttcacagca gattgctgga caaactcgtc cattgcttga
    10921 ccctcttctt tttgaacaca ccttgttttt ctgttatata ccgaaaaacg gccattcagt
    10981 tcaaaacaag gctctaaaca aaaacagtcg atcacccatt cattgatgga tagcgacgat
    11041 tcgtttacta tttatcctgt ttctaccttt tcggaagaat ccggctatgt aatcaatact
    11101 gattccaaag atacacttag gaagtttgtt caaaagtttc gtcaagaagt ttttccaggt
    11161 gatttttttg ttgtttctga ttcttttgca atccaagata tcaatgaaat attccaagag
    11221 atcagaaatt gcaccaaagg acaacgtttc tatcataagg gaaagaacct tgcttatcag
    11281 catgaacttc ttccgttatt aaccaaccac gagcagcaag caagtagtta tttaacaaaa
    11341 atcgttcata aagactacgc tttagctgtc aaagaacttg agaatttctt cacgacgata
    11401 ttggaacagc ctattgatcc agcttatttg aaacatgagg caatcaatcg tttctatgca
    11461 ttactcaacg ctttggctga cgagcatgtt gggaatgaag aactttcttt gctcaagatc
    11521 gcccttcctt ctttattagc cgatgctgag tatttcgaag atttttctat tcttgtcaaa
    11581 gaaacacttg agaaactcac ccacttttct gcaaatgcaa aaaaagacaa tttcgatatc
    11641 ttgactttga tctacgagtt cgtccaggaa aattatgaga aagaaatcaa tttacaactt
    11701 ttatccgaaa aatatcattt ttcatatagc tatctctcag ccatctttac tgaaaaattt
    11761 ggtatcaact tttcaaaata tctaaaaaaa gtccgtatca gtaaagccaa gctctttttg
    11821 acggaaacac ggcttacttt gacggaaatc tgtgagaaag tcggctatac cgaactgggt
    11881 tatttctcca gagtttttaa agaagagaca ggaatgacac cttctcaata ccgtaaagga
    11941 acttttgtaa aatgagaaaa aaaatcaaag agcatgaact ctttgccaaa cttctgttca
    12001 ttttgttcat cggtcttttt ctgcaagcca tcgtcatttc tttgtttatt tatcatcgat
    12061 caagggatgc ctatatccag ctgttcaatc aatcgaatga tgtggtacta aaaaagattc
    12121 aatctgaatt tgaaagtttg aatgatacaa tcgagaatac attagccgct tttgattcta
    12181 atccagctgt caaaagctat ttttccaatg atccagccca acatatggaa cagcttcagc
    12241 aactaagaac aatccaaaaa atgaatgact ctctttcaaa aatccatcca atgatcgatt
    12301 acgatgtatt gatctttgga gaaaatggaa gaacttttgt cggaaacgac atgctcactg
    12361 ccgtatctgc cgattctttt tttcaatctg cgattgctca gagagtgaat gaacgtgctg
    12421 ctgatacaca aatgcttttt gcacaccacg gattgactct tcgcgataag aaagcaccca
    12481 gcgtcttctt tgtcaaaaaa ctgaaaaata cattgaatca cgtttacgga tatgctgttt
    12541 tatcgatctc atccaagcaa ctagcgaatc tttttcaatc tgtagtaaat cctgagatat
    12601 cgaagatctc cttcatgaac gaagaacaga cgatcatcgc atcaaatgaa gaaaatacaa
    12661 ttgggagccg atcaatgatt ttcaaaaaac taacagtagg tgaaacaacg atttcaaatg
    12721 gacagcagtt gacccgtctt cctctgtacc gccaggattg tacattactc agtcaagtcg
    12781 atcttcattc tttatctaac caaatgggcg tgatttttcc gattattttg tataatatcg
    12841 tgacacttgc ttttgctggc tcattggtat ttatgtattt ggatcgacac acaaaatcga
    12901 tctaccgctt gattgattcg ctaaaacggc tagaggaaga tggcttaaaa gctactgttc
    12961 ctgtacaagg aagccatgaa gtccaattgc tagggaatac gatcaatcaa ttgctaagta
    13021 atgctcagca tcattatgaa cagacgatcc aagacgaaaa gaagaaacgt tcgcttgaaa
    13081 tccaaactat gcaagcacag atacagcccc attttattta taatacgctg actagcttga
    13141 aatttttgat ttggcagaaa aaaacagatg aagcagttca aggaatcgac agtttcatta
    13201 ctttactaag aagtacgatt gggaaaaaag aagaagtgat tccagtcaag gaagaaatca
    13261 aaagcgtaca aagttacatc gatcttttga gtatgcgata tggcgaaaga atctcggcta
    13321 aaataatgat tcctgatgaa ctgcagtcat tatctatgcc gaatatgatc cttcaaccaa
    13381 ttatcgaaaa cgcctatctt catgcttttc acaataaaac gcaaggattc atcacggtgt
    13441 acggcaaaac aaaaaatcag cttcttttat tcgaagtgat tgataacggc gatggcttcg
    13501 atactgaaaa taccaaacaa aaaaatgatc atttttcagg aatcggcatc agccatgtag
    13561 atgagcggat acgcctaatg tatggcgatg catatggctt aacgatccaa tcagtgattg
    13621 ggacaggaac gactgtaacg atttgtctac cgcttcttcc taaaaatcaa tcagaatatc
    13681 aaaaataaag aatagccaag cgctgatttt tttattttaa gttaaataaa tcaatcagta
    13741 ttcttgactt tcatctacgc gtctcttcta ctgtcagcac tggataacag cccaatattt
    13801 gactttctcc tccgagcatc ttgatttctt ggagcgcatt ctctactaat gtcattggac
    13861 ggtccaacaa caaatcgatc acgaaaaaat attctcccaa gctagttttc aatggtcggg
    13921 attcaatctt acttaagttg atttcccgcc agccaaaagc tgccagcatt ttatgaagca
    13981 tccctggccg attagcaggt agtgtcaaga tgacactcat ttttactgga gctgactgct
    14041 ggctggtcat ctttcggtca ccgacgatcc aaaaacgtgt ctggttcaac tcattatctt
    14101 ggatattctc agctaagatc tccaatccaa catgctgtgc tgtttctaat gaagcaatcg
    14161 ctgctgcgtc ttctttcgga tgttctgcta catacattgc tgcagcagtt gttgattctg
    14221 ttgctactaa aggaacatta ggataatgcg tttctaaaaa ctgttgtgat tgtgctagtg
    14281 cttgtggatg tgaaaggatt tttgtaatct ttgttgctgg attgcctaaa agctgctgct
    14341 tgataggaag aacgatttca cttttaactt cgacttctgg atgtttggaa agaagatcga
    14401 ttgtgtgatg aacagctcct tccaatgaat tctctacagg aacgacagcc agatcgactt
    14461 gtttgcgaaa caaagcgcgc aaacaaacag ggatggaagc atacgcagtc aattgttctg
    14521 taggaaatag ctgacaagcc gcctgataag tgaatgaact ttctggccct agatagctga
    14581 cgttcatatg cttttgatcc tttctataat ggtatcgatg atcttttctg gtgtttgatt
    14641 ggtcgtgttg attcgatgtg tcgcactttc ttcataccaa gaaagtcgtt gtgccaataa
    14701 tgcttttatt tcttctcttg tttttccaag agctaaaggt cgaatagacg tttcatcttg
    14761 ttggatacga tcaactagac agtccgcttc tgcctctaag taaagaacaa tcggctgtgt
    14821 ttttaaaaaa cgccgatttt ctgcactttg gacaatccct ccaccagtcg cgataatttt
    14881 gtcttcattt gacagctttc ttaaaatatc tgactccact tttcgaaagc ttttttcacc
    14941 gtaatagtga aaatattcgg caatcgacaa ttggatatgt tcctcaatct ttgtatctaa
    15001 atctacatac tctttttgta aacattgagc taacccttta ccgattgttg tttttccagc
    15061 gcccatgaaa ccaatcagca aaatgctagc catgagcttc tcctcctgac acaagttctg
    15121 ctaaatcgtc aaaaaatgcc ggataagaga ttgaaacagc ttcagctcct tctagttcgc
    15181 aaggtccatc agctatcaaa gcagctactt gcagcatcat accgattcga tgatcgcctc
    15241 tgctggacac ttttgcgcca tgcagaggtg tcggcccatg gatgatcaga ccgtctgctg
    15301 tcgcttttat ctttgctccc attttctgta attcttctgc caccgcatca attctgttgg
    15361 tttcttttac ttttaattct tctgcgtctt tgatgatcgt gattccttct gcttgagtag
    15421 ccactaaagc tagaatgggc aattcatcaa tcagacgcgg aatgatttct ccaccaatcg
    15481 ttgctttctt caaatgactc gtcttgacac ttaaatcagc cgattggttc tgttcattgt
    15541 gattcgtctg ggtaatcgct gcgcccatct cctccaacac atctaagata ccagttcttg
    15601 ttggattgac cccgacattt tttaacaaca gttggctttc cggaacaaga agtcctgctg
    15661 ctagaaagaa agcagctgat gaaatatcac ctggaacagt tatctgttga ccagttaatt
    15721 tttgcggtcc ggtcactatg atcgttttat cttccactgt gatccttccg ccaaattgac
    15781 gaatcatttc ttccgtatgg ttacgggaac gttctttttc aacgattacg gatgtgcctt
    15841 ctgcttgtaa tgccgcaaac agcaaagcag atttgacttg cgcactggct acaggcatat
    15901 tgtatcggat aggtgataaa gagcgggttc ctgtcacagt gattggtaga tactggtcat
    15961 tttcttctcc ttgcaagtca gcgcccatct cgcgtaaagg ttccatcact cgccccattg
    16021 gacgtttaga caacgaagca tccccgaaca gagtagtcgt aaaaggctgt ccagccaaaa
    16081 tacccatcaa taaacgtatg gtcgtaccgg agtttcccat gtcaattgat tcggttggtt
    16141 ggacaaatga gtggctaccg cgaccatgaa tcttgatcac ctcttcctct tcttcgatct
    16201 cgacacctaa ttgttggaag gcatgtaaag tactcagaca atcttctgcg cgcaaaaaat
    16261 tttgtatagt cgtcgttcct tcagcaattg ctccaaacat gatgctgcga tgggaaattg
    16321 atttatctgc tggtatccta acagtccctc ttaatccatg tatttgttgt aataattgca
    16381 tttatccagc ctcctatttg atccagcaat gataactggt attagacata atgattcttt
    16441 ttgcttgtaa caaatcctgt tggtttttaa aactgatttg cagtattcct aaaatatctt
    16501 ccctggtctc ttggatcttc aaattaatca aagaaactcc cgcttttccg atcagtccag
    16561 ttacttctgc aataacgcca ggttcatctg gcacatcgat aaataaatca aaaaaggcag
    16621 gaatcgcgcc ttgcttatgg acaggcaact ggtctcgact ttcttttgcc cgattgaaaa
    16681 aagaaaagat ttccgattgg ttctcagtca tgatccattc tgctatttgt gtcatttctt
    16741 gttgccaatc tgccaatcgt tccaataata tctgcttatt gctcaacaaa atatctgtcc
    16801 acatgacagg atctgaggaa gcgatcctag tgatatctcg aaagcctcct gctgccagtt
    16861 gtttggctcg cggatactct tgattgaatt gttctgcctg attgactaag ccagctgcca
    16921 gaatatgtgg aaaatgactt agcattcctg ttatacggtc atgttctctt gctgtaagag
    16981 ttacatattt tgctttggtt ccgcaaaata gttcttgcag tttttcaaca tcttttttcg
    17041 tcttttccaa aggtgtaaag atgtaatagg cattttcaaa tagtttagca tctgctgcct
    17101 gtatacctga tttatgagaa ccagccattg gatgtccacc gacaaaccga agatttttct
    17161 gctcagcgga tgtcacgact tgctgcttcg tactgctgac atccgtaatg atgacgtgat
    17221 cagacaagtt gattctttca aggacatcaa gataagctaa ggttgtacgg acgggaacgg
    17281 ctaataagat aacgtctgct tgttctgcac ctgtttcaaa atcacttgca acgtaatcaa
    17341 cgatctgctg ttctactgct tgctcacggg tttttgactg actatcccaa ccaactacta
    17401 tcagttctgg attggtctga cgaatacaaa gtgccataga agcaccaatc agtccaagcc
    17461 ccacgataaa aacatgttgt gccatcttat ttgccccctt tttagtaatg tttcaagtag
    17521 tcacggtact ccttcactgc agctttcaac tcaacaagcg aatcacttgg gaattttgac
    17581 aatagctctg tcgctaccac agtggctaca acattttccg caacgacgct agctgcagga
    17641 actgctgtac tatccgaacg ttctacactt gctttgtaag gttcttttgt atcgatatca
    17701 acactttgaa gtggcttgta caatgtagga atgggtttca tgaccccgcg aatgacaagt
    17761 ggttcgccat tggtcattcc gccttcgaag ccgcctagat tattggtacg tcggaaatag
    17821 cctctttctt cttcccatag gatctcatcc atcacttctg agcctggtaa tgaaccagaa
    17881 gtgaatccga ccccaaattc tacgccttta aaggcattga tactaaccac agcttgtgcg
    17941 attttggcat ctagcttcgt gtcccattgt acgtagcttc ctaatccagc tggaacgcct
    18001 ccaacgagca cttctacaat tcctccgatc gtatctccat ttttctttgt ctgatcgatc
    18061 aaatctttta ttctttgctc aactgctgga tcaacaactc gtacttctga cgcttcagaa
    18121 atttctttga tttcttgaat cgagatattt ccaggtattt ctgcttggat tccaccaaga
    18181 caaatcacgt gtcctgctac gatgatgtca agttcttcca gaattttctt ggcaattgcg
    18241 ccgattgcta cacgcatggt cgtctctctt gcagaggacc gttcaagcac attccgaaga
    18301 tctcgatgat ggtatttcat accgcctacc aaatccgcgt gtcctggacg cggacgtgca
    18361 actcgccgca gtgattggtc tttttcaggc acttctgcta cagacatgac agttgtccaa
    18421 tttttccaat ctttattttc taccactaaa gtgacaggtg aaccaattgt tttgccgtga
    18481 cggatgccag acgtgatccg aacacgatct ttttcgatct tcatccttcc tcctctgccg
    18541 taaccagctt gtctgcgagc gagttcgtga ttgatttctt cttctgacag aggtaatcct
    18601 gctggtagtc cttcaataat ggctgttaat tctggtccat gggattctcc cgcagtcata
    18661 taacgcatct tacagtcctc ctaagtaatc tttgaatgtc tctgtgggaa caggaacgat
    18721 ttttgcttgc ccaatctttt ctagcaagat gatattcaat tggcttcctc ttgctttctt
    18781 atcatgggtt atcgcgtgga ataattcatc tggatgactt agatcgtacg taatcggcag
    18841 atggaatttt tgaagtatcc tttctaaagc agctgtcgat ccagttggag acaagccgat
    18901 tttttctgca tgagaagtga tggctaccat tccaatagct actccttctc catgactgac
    18961 taccccatat cctgctgtat tttcaattgc atggccaatt gtatgaccaa aatttaaaat
    19021 aagtcgattt ccttggtcaa agacatcttc ttccactact tttcgtttga tttccaacgt
    19081 agcagtaatg attggaacag catgttccag aagatctttt tcgtctttca gctcgttcag
    19141 ctgtttccaa agcgttgcat ctgctatagc tgctgatttg atgatttcag cgattccttc
    19201 tcgtaaacga cgtaccggca aagtttttag aacctcagga tcaatcaata caccagctgg
    19261 ctggctaaat gttccgacaa ggtttttcgc tttgtttgta ttgactgctg tcttcccgcc
    19321 aatactagaa tctacttgtg caagtagcgt agtcggaatt tgaacgaaat ggatcccacg
    19381 catgaacgtc gaggccacga atgctgctag atcacccacc attcctccac caagtgcaag
    19441 tatcccatcg ctgcgggtaa aatcattatc tgctaaaaaa tcgtataact cagctgcttg
    19501 ttctaaagat ttactggtct cacccgccgg tacgattgaa aggactggtt caaaattagc
    19561 ttgtctcaat tggaacatga ttttttctgc atatagtgga gcaacgtttg tatcagtgat
    19621 cacagctact ttttgaggtc tccaaatcgt ttgaagccat gatccagcct gttctagtgc
    19681 gcctttttca atatgaatat cgtatttttg gttatcaaca tgtacagtaa gcattcgttt
    19741 cacttctttc ttttaaaaag ctggaattaa tcgagtgtct gaatcttttc cattgcttct
    19801 ttcatgatcg caacttcttt catcatgcgt gagtaagtct tttcattcaa cgattgggga
    19861 ccgtctgacc aagcatttgc aggatcagga tggatctcta cgatcatgcc atctgctccg
    19921 cttgccacac ccgcacgagc cattggtggt acaagttccc aaattcctgt accatgactt
    19981 ggatcaacaa tcacagggaa atgagttaat tttttcatca atgggacagc acttaaatca
    20041 aacgtattac gtgtcgctgt ttcgtaagtc cgaatcccac gttcgataaa aatgacagga
    20101 gatttgtctt cgactgcaat gtattctgca gcattaagcc attcattgat cgtaccggaa
    20161 attccacgtt tcaatccaac aggtttacct gttttaccga cagcagatag cagtttaaaa
    20221 ttttgcatat tacgtgcgcc aatctgtagg atatcgctgt actcagccac catatcgata
    20281 tgcccttcat ccatcacttc agtaatcact ttcattccaa attcatcagc cgcttgacgg
    20341 atgtatttca atccttcttc ttctaaacct tggaaagcat atggagaagt acgtggtttg
    20401 aaggcaccac ctcgcagaat ttttgcgccc ccggctttgg ccattcgcgc acattctctg
    20461 atttggtcta atccttcgat agaacaagga ccagccatcg tcacgaaact gccgtcacca
    20521 atttttactc cgtctacatc cacgactgta ttgccaggat ggaattctcg agaagtcaat
    20581 ttgtatgtgt ttaaaatacg cacagcattt tctactcctt catagcgtgt aaatgctcct
    20641 tcttgtaaac ttcgcgtatc accttttagt ccaatcacta ctcgttctgt tccttgatcg
    20701 attgagactt tgaatccttc tttttcgata tgatcgacga ctttttttat ttgttcttcc
    20761 gttgcttctt ttttcataat tactatcatt gtttttcccg ctttcttttg tttacttatc
    20821 tatcttggct gattttctct actttttcaa ggtggtttta cattaattcc tactaaatga
    20881 ccgtgtcctg ttgttctcat tttcgctcct tctttcctac ttatgaccca gataatcaac
    20941 aaaaaaagat gtccctgtag gaaattgcct ctacagtgaa catcttcaac gagccttaaa
    21001 aagcgctgca aaaatataaa atagccacta tagatgaatt tctctacgtg gctgtaagtc
    21061 gtgtttaaaa atctttagcc atcatagata caaacctggt ttgtttgtac ccacgaagtc
    21121 atgaatacaa acctatctaa aatagctaaa gtaatacgta ttcagttttg taagttttct
    21181 agatttcata aaaaatcgct ccaaactgat gtaagaattg tacctagtat agacataaaa
    21241 aaatcgactg tcaatcattt ttatgaaact ttttttaatt ttctgatagt taactttgtt
    21301 tttcagaaaa ataacaattg attttattga agaaaatagt catgaacaaa agtatctgag
    21361 aactttttta ctctcttgtt catgacatat agaataaagc agctttgtct tgtaaactcc
    21421 gtttttcaaa taataatcta aagattgcta ctgaataata aagccgattt catggtcctc
    21481 ttcgctgctg attcggtaag gtgactcaac atccgctccc cagctattga tacccccgac
    21541 tcctcttact tttgcaaaaa tcgtaagaac cgttcgtctt tctggcggta gttcttctat
    21601 atgtgttgcc tgttccagtt cttctgcttt gaatggtaaa gcggaaaaag caaatggcgt
    21661 atcctgctgt tcaaatcgca acgaaaaatc ttcttgatct ttatccgtat tatccaacgt
    21721 ttgatttctt gttagttcta cccatttcgt ttccatatgc atgccgcatt cttggggaac
    21781 aaggtatggg gaaacgggga gtcctgccac tttataaata ccttcgacac caccagccat
    21841 ccgatccgga tatgtttccc ctgataggcc ttcatactca aaaaattttg ctttcgttgg
    21901 cataatgaac cttaaaccca atgcgggtaa ttccggtaat cctttttgtc cataataatg
    21961 catattgaca agcattttac cctctccagc gatttggtag gaaattgtca cttgggtttt
    22021 tggtactgtg ttgatttcca gtgtaaaaat gatttcaacc gtttcagcgt attcattact
    22081 tgaatattga ttatttactg gagcaatcgg caaatcgatc tttttattgt ccaccagtac
    22141 ttctatttct ttgacttttg gatacaatcc ggctcctaac cacataccgg atttaaatcc
    22201 aaatccgcta cctctgtcat tatcggtcgt tgctcgccaa aaaactggaa gtgtttcccg
    22261 gtatagccat tcttttccgt ttttattgag cgattccagt ccgcctcttg tataagaaaa
    22321 aatataatgc gtattccccg atttgacacc taatgtacag tcgccgaaga cgattgcaac
    22381 tttttccgta tccatatgac ttttatttac tgtatttttc ataataatat cttacctcct
    22441 gcatggcggg tttttcttgt ccatctgcaa aaacgatccc attccctgag aattcatagt
    22501 cacttggttt atcgtcaaag tcaccgccat agcgcatcac ttctctgcct gtgattggat
    22561 cagtcacttg gatcgcctga tctttaaaat cccatatata gcctccttgg tacatttcat
    22621 aacgatcaag caacgtcatg taagaatcca ttccacctaa cgaattcccc atgtcatgca
    22681 tatattcaca taagatgaac ggtttttccg ctttattttc aaggtagtca atgatctctt
    22741 ctggtggcgc atacatgcag ctttttacat ctgaaatcga atcttcgaat gctggatttg
    22801 gaaaattccc ttcataatgg acaagtcgat cggggtctac ttttttgaag taatcgttca
    22861 tttgtcgaat gttttcttcc gcataagatt catttccaag agaccaaaaa agaatacttg
    22921 gatggttttt taatgtttcg aaattcgttt ttgctcgatc aagaacagcc gcctgccact
    22981 gaggtactgc acctggtacg ttgtaagaag gctcaaatgc gcccattttt tgccatgagc
    23041 catgggattc gaggttcgtt tccgccatca catggattcc tgcttggtcg cataaataat
    23101 accaagtgat ctggttgggg taatgagatg tccgtacgcc gttgatcttt gcatttttta
    23161 tgatagccaa atctttttcc atatcttcta tagtgatggc gcgtcctttt ttcggatgcc
    23221 actcatgtcg atttaccccg ttgataatca tccgttttcc attgaagtag atgattttat
    23281 ctttgatttc gattttacga aaaccgaagg aatagtcaat atactccgtt actctttttt
    23341 cttcgtctat cgtctccaat tcaagcttat ataaattggg ctgaaaatgc ccccacagtg
    23401 ccacattttc aaatccatcg atgcgaatcg tcatttgctc ttttaacgga tattgctgtt
    23461 gatagatttc cttcccatca ggatctagta tcgctacatt cagctgactg tttttggcta
    23521 cttgctcaaa agacaaatct acttcaagtt tgccattttg acatttgtca tctaagcgag
    23581 gtttgatcca aagatcttct aaatggtttt gtcctcgagc aactaagaag acatctctga
    23641 agatcccaaa aaatcggaaa aagtcctgat cttccaagaa tgcagctgta cttcttttgt
    23701 aaacctccac agctagaata ttttcttctt ccaaataagg tgttaaatca aattcagacg
    23761 gagtgaacga atcttcggca tacccgataa aatgaccatt caaccaaata aacattgctt
    23821 gttctacacc agcaaaatga atcgagattt gttttccctt tagaccttct tctaatcgaa
    23881 aagtcgtcaa atacgaacca accggattgt ctttagcagc tgaaaagatc ccttccgcct
    23941 ctttctcagg gcttgtcgtg aaagggggac ggcgaaatac atgtccctcc cacggataca
    24001 tcgtattgat gtaatggatc ttgtcataat ttgctaattc aatatgacta ggaacagtga
    24061 tttcatcaaa agaacaggca tcaaaatctt ttttataaaa tgtttttggt cgtaccgaag
    24121 gatttggcga ataacaaaaa cgccatgatc catttaaaga ctgtttaagg gaggatgtgg
    24181 tttctacttc ttcccttgtt gcataacaaa catgatcgct atgtgcattt gattggttca
    24241 ctctaaaaac ttgtggatca tctaaccatt ttatatttgc tttcatactt ttttccttct
    24301 ctctcataac tttttgaaat gaagcagttc tgtttggaaa tcaccttgaa aatcggggaa
    24361 gtaaagtcca actgtcatca attcggcccc cgaaaatatc cgcttcgagg tctgttcctg
    24421 ataaagtgcc tcatcatcta accccatcag atatacttca tggaacgctg gttgagctga
    24481 agctagtatc ctccctaaaa acacaatagc ttcatcttgt ttaggagaca caaacatcca
    24541 tgcggcaata ttttcttcaa aaggactcgc caaacgataa taatggccat actggatcac
    24601 cggacgaatc gtttgatagg atataatttg gtcttttact tgcttttttt cttcaggaga
    24661 cagctttgtc aaatccagtt cataaccaaa aacagcgctc atagctgttt ctcctcttgt
    24721 tttcaacgaa gtagatctgt cggtctgatg atttggtaca gctgatacat gagctgtgat
    24781 ggacgaaatc ggataagcaa ggcttgttcc atattggatt ttcatacgct ccactgcatc
    24841 cgtgttatca cttgtccatg actgcggcat ataataaata aacccggcat caaaccgtcc
    24901 acctccacca gaacagcctt cccataagat ttccggataa tcagttgtca aatgttcaag
    24961 tagttcataa agcccgagca tataacgatg cgctacttct ccttgtctct gtgggtcaag
    25021 ggtaatcgaa tagacatctg aaagcgaacg gttcatatcc catttgatgt agtcaagagg
    25081 aatcgtatct aaaatctttc tcatttgttt ctcaatcgtc tgccgaacat ctaaccttgt
    25141 aaaatccagt acatgttgtg atctggaagc agaaggcatt ctacctggct cctgcataag
    25201 aaaatcagga tgtgttcgat acagttcaga atcaaccgag atcatttcag gttcaaacca
    25261 taaaccaaat ttcaatcctt tgctatgagc ataatctgct atttcacgta atccatttgt
    25321 caacttgcct tcatactcga accagtctcc taatgaagaa ttgtccgcat ctctttttcc
    25381 aaaccagcca tcatctaata cgaacatttc gatgcctagc tcctttgctt catcaatgat
    25441 cgccttgatt ttttcactat tgaagtcaaa gtaagtggct tcccagttat tgaccagaat
    25501 cggccgttca gcaaattgat gacgacctct tgcgattctt tcacgtagca actcatggtg
    25561 gatctgactc attttgttca atccctgact tgagtaagaa aggatcattt caggtgtttg
    25621 aaaagactgg cctgcattca attcccacga aaaacgatgg ctgttgatcc caccgacgac
    25681 tctcaattga ttgatctgat ctttttcaag ttcgaaagaa tggttgccgg aataaacaaa
    25741 ttgaagtccg atcgcttctc cagtattttc tgtcgtatga tgatgaacta aagcaataaa
    25801 gttattcata tggtgactgc tcgttcctcg acggctttca aatacttttg ttccagataa
    25861 aacagattgc cgtgaaatct gtctttcatt gacatgtgct cctggcaaag caatgacttc
    25921 atcaaatcgc ctagtatggg caaagtctag ctggaaacta gccgcttttt ctagtttgat
    25981 tgtttctccg gtttcatttc ttatctttac agatcgtgtg atcactgctc tatcagtaaa
    26041 tatggtatag aacaaatcga aataaatcgc tgtttctcta tccttaagtg aaatcaccaa
    26101 cgtttgtgcc tctgacttgt ccatcacata agtttgaggc aagccttcga tctctggctt
    26161 cccatctgta atataatggc tgtcatatct cagatccagt aagtctgatc cattcttgcg
    26221 ctgaatgatc gatgcgggcg ttcgaaagtc catgctgcca tgagaagagt attcttgcgg
    26281 tagtgtatct ttcgacagag accggtcagg atttaaggga acatttcctg aaaatccacg
    26341 atctcttcgg ggataatttt tattgtcttt gtactgcttg actggtttgc caaaataaat
    26401 atgcgccaat acattgcttt cttctatctt tatcagataa gaaagaaatg tatttcgtaa
    26461 atggaagact cggctctttt catcaaataa gatgaaattt ttatctgttt ttctatgcgt
    26521 aatatccatc atttcactcc caacaaaacg gaaaccgttt actttttttt ataattcctg
    26581 tatactgttt actaaacaat tttgtcaata aaaatttttt atttttttga ggagtgtgta
    26641 aaaatggttg gaattagaga tgtcgcacga aaggcaaatg tctctcctgc tacagtttcc
    26701 cgtgtgttga ataacgatca gacgatccat gtggctcaga ctactcggca acggatagta
    26761 gaggcagctg ctgcgttgga ttatgacatc agtactcgta aatatatcaa aaagagaatg
    26821 ccttcgattg gtgtgatctc tacgatcggc aaagcgagcg aagaacagga tatctattac
    26881 aaacatttgc gagtagggat ggaagaagaa gctcgacgtc tgcatttagg gatgaatcga
    26941 atctataatc tatctgataa tcctaaacaa tgggaagatc tagatcagtt aggtgcggtg
    27001 atcgttgttg ggactgttac gaaagaatcg attaccgcct tgttaaaaca aaacatgcaa
    27061 atagtcgtcg tggataaccc tgacatccag caagaggtcg atatggtgta tgcagatctt
    27121 gaacggatga ctaaaaccgt actggaattg ttcttagaaa atggtcataa aagaatagcc
    27181 tatatcgggg gatataaagt cgatatggat gaattcggtg ggaagcagat ttctataaat
    27241 gaaaagagga tgcgtgccta tcagcatttt atgatcgatc atcacttaga aaaatatatg
    27301 tcttatcatc taggtggctg gacagaaaaa caaggtgagg tacttgcgac taatctatta
    27361 gaaaatgaat cccctcttcc tactgccatt cttgtcggga gcgatccaat ggcaattggt
    27421 gtctatcggg cattgaaaca aaaaaatatc caaattggag aagagattgt tgtagcaagc
    27481 tttgataata tcgagacaat cgcaaacctc tcccctggct tgaccagtgt tcaaatcaat
    27541 gcgaaatcaa ttgggcaagc agctgttcga atggcattgg agcgaattga cggtatacgg
    27601 gacgaaccaa tcattttgac ttatcctgca cgattgattg ttagggaaag ttttatccca
    27661 agaacaaaaa ggtattgaaa gagaggttgt gaaaagccgc ttcgtttctt catactcaaa
    27721 acgaaacggc tttttcacaa cctcttactt atacttattc acaaaatcca atgtctgctg
    27781 gtaacaattt tgcgattctt ttagcgtgta atcaaattgg tattcatggg taatctcttt
    27841 ttttgtatct gtataaaaca aggattgcac gggtaccttt aattctgtca accgattttc
    27901 taatgcttga ccttgctcct ggaatgagta tgcatttccg tcagttatat acgttggcgg
    27961 aaattcctta gaaacatggt cagccacact agcttctttc aattgctcac tcgttttcca
    28021 atcttttgtt ccaatcaacg accacgcaac ggttttgacg aaaaacttca taaatcgatc
    28081 atccgaatgc tgctgtgctg ttagcttcaa atcaacaggt ccgcaataag aaatggttcc
    28141 tttcagacta tcttttggaa gtagctgttt gattttcatt tgtttagcat acgcatcatt
    28201 cgtttgtata gctgcatatt gcaatgcgat ttgtgaacct gcactatctc caccaaaaaa
    28261 taattgttcc atgtctagca atttgtcgtc tttttcttca gctaataaat ctttgatcag
    28321 ttcattcact tgaagaactt gattagggta ttctgaatca ggcgctaatt cataattcat
    28381 cgcaaccact gcgatgttgg cgtctgaaac taattttgtc gcaaattctt tgacacccga
    28441 tttatctccg ccgacgaatc ctccaccatg tacccaaaat agaacaggaa caggcccctt
    28501 gctgtttttt ggatagtaga tgtcataagt attctcttta tacttggact catacgtcaa
    28561 atctgttttt ttcgccacgt ttttttctgc ttgatcgaac gtttttttat cagtgatttg
    28621 aacagtattt gaaaacatat ggttgatcaa gaatgctcca ggtctggggg aaagctggaa
    28681 ggcaagaaag attgcaaacg cgatcccaag aatgattccg aagaaccatc cgataaattt
    28741 aagaattttc tttttcataa ggctccctca gtagttagta ttaagcattc tgattatacc
    28801 atagtgttat ttcctatcaa gctctcctag atgatttaat gattttcccc aaacttcttc
    28861 tgcaatgctt ttgacaaaag aaattttttc cattgttctt caaacaaaac attttcttct
    28921 tctgttgaag caaatccaca ttgaggactt aagcacaaac gatccaaagg cagatatcgt
    28981 gtcgcttcgt agatccggtc aatcacttgc tggtgctctt ccatttcgcc atttttcgtt
    29041 acagtcaaaa tctaagacaa ctaatttatc ttcagagact ttctctaaaa gaacaaaact
    29101 cccagcacgt tcactatcgt attctaaata ataggcacgg acattttctt gagtaaaata
    29161 atgggtctgc tacgtcgtca tcccccctga tgccgccaaa atcgaataat aatttccgcg
    29221 tcaaacatgt atatttagtg ttgaatcatt tgagaatatg aaaataaaaa tctttttgat
    29281 caatcagtta atcaaattga aataacgaaa aatctgagac agaatgacgt tcattctgac
    29341 ccagatttga ttttgcacaa ctcaacagtc cttattggca aatcgccaaa ataatgtaga
    29401 ctaactaaaa agactaagaa acaataacaa aagttaacac ttcttagtct ttctttagca
    29461 cgtctattta ttaaagattt tagggccttt tctcatgttt ttacgatcat tcttaggtgt
    29521 ttccatttgt ttggatcggc ctccaccttg tttctttttg atcaattctt tcattttttc
    29581 tttttggtcc atttttattc tcctttaacg aatgaagcga ctaattcgat atgatgggtt
    29641 tgcgggaaaa gatctacagg ctgaactttc ttcacggcat aacctgcttc tgcaaataac
    29701 ttagcatctc ttgcaaaagt tgccggatta catgagatat agacgattcg ttctggtgac
    29761 atttcgattg ctgattcaat aaaatgatga tctaatcctt ttcgtggagg gtcaactacg
    29821 atcacagatg gtttgatccc tttttcttgc cattttttca taactttttc tgcttttccg
    29881 acttcataat gaacattttc gattttatta tttaaagcat tgaactgtgc atcttcaatt
    29941 gcttctggaa taacttccat cccatacacc ttatccactc tgtctgctaa agacaagcca
    30001 atcgtaccta ttcctgaata agcgtcgatc acaatgtctt ttttatctaa ctcggcaaac
    30061 tcgatggctg tttggtacag atactctgct tgttgagtat ttacttgata aaatgacttt
    30121 gctgagattc gataggtttt tcctaatagc tgatcttcga tatagtctct gccatataaa
    30181 actttttctt tgtctcctaa aatcacattt gttttttctt cattgatatt ttggatgacc
    30241 gaaaccactt ctggcaattc ttcatgaata gcttcagcaa tttccttgga tttaaagaat
    30301 ttttctttac gagtaactaa aacgaccatc atttcatggg aataatgacc acgacggata
    30361 atgatgtgac gaagctgtcc ttcatttttt tcttcattat atcctcgtac ttggaaccgt
    30421 tgcaagatat ctctgactac tttgattgct tgatcgatga caggatcttg gatataaaaa
    30481 tcttcgatag ggactagatc atggctattc tttttgtaaa aaccggtggc taactgtcca
    30541 tcgatacggc gaacagggat ctgtgcttta ttacggtagc cgaatggttc tgccattccg
    30601 accgttggaa gtacaggaac ttctggcatc ttagcaatct tcgccattac atgctctact
    30661 tgtttttgtt tgaagacaag ctgttgttca tacttcaaat gggctaaagg tgcgatacct
    30721 atgcgaagta atgcattgtt ttctacaggc tgacgatcag gtgattgtgt aataaatgtt
    30781 tcgacttttc catatccaaa cttattgcct gcttttacta ctagtatttc tacttcttct
    30841 ccagggagtg cattttcgat aaataatgga taaccatcga tttttgctac ccccattcct
    30901 tcataactca agtcgacgat ttcgactgta tatcgttcgt ttttctttac tatttgctgt
    30961 tctgtcatat tttgctcctt taagcctatt tttcaatcct actgaactct actataaatc
    31021 agctaattac acaactaaaa ccggcactga ccttactacg ttcagcaccg gcttttgatt
    31081 aattcttttg attaggtgtt tcgtctgcta tttttccgtc accgtcgata tcttcatccg
    31141 ttaaacgttc gacttctttt acgaattctt tgctgacaac ctctaattct tcttcatctg
    31201 tacctgtgat tgcttcatca ggaatctgat cggtattggc aaagaactca atgtgttgat
    31261 gaagattttg gaaatgcatc ggtgcttccc cgccgtattc accatctaag ttgatcatta
    31321 ctggtcgatt gatatcatct tctgtttcaa cgaacaaact gcttgttttg gtatagatta
    31381 ttcttggatc atcgacatgt cgaccgccat tcagcatgag tgctgcaata tgcaagatct
    31441 caaagatatt tgctgtcttc acaatgatta aagaaaactt cccatcatcc aatttggcat
    31501 ctggcgcgat ctgttcgaat cctccaacag agttcgtcag ccctaggaaa aacatcgaag
    31561 catttccgat atattcccct tcatcatact tcaagcgcat tttgattggc tttactcgag
    31621 ggagcatctc tgctcctttc gcaagataag caagataacc aaaaatactc tttagttctg
    31681 atggaacttc ataagtcaat tctgtcaaat agccacctgc agcgatattg atgaagtaac
    31741 tgttatcaga aaggccgatg tccattttta cggtttggtg tttttttatc acttctgctg
    31801 ctgctttaat attatctcta ggaattttta atgctcgagc atagtcgttc gttgtaccag
    31861 ctgggatgat ggccatttta ggacggtgtt caagcggagc gattccatta accacttcgt
    31921 tgattgtgcc atcgccacca gctgccacta ctagatcaaa acctagttct gcgactctac
    31981 atgcttcatt cttggctgaa tctggttctg gtgtagttgc ataggcgctg gcttcaaatc
    32041 ctgcttcttc taatgcctgt aggatatccg ccaaattttt tttcaataat tcttttcccg
    32101 aggtggggtt ataaattacg cgtgcttttt tcattttcag cctctatcgt ttcgctaatt
    32161 catctttaag tagcttattc acgacaccag gatttgcttg tcctttagtt gctttcatga
    32221 tttgaccgac taggaagcca accgcacgat cttttccatt tttgaaatca tcgacagatt
    32281 gttggttgtt atccaaaatt tcattaatga ttggcagtag tttgttcgga tcagataatt
    32341 gcacccaacc atttttttca actacttctt gggcttctcc gccatttgtg atcaattcac
    32401 ggaagacttt tttggcgatt tttgagctga tcgttccatc ggcgatcaaa cgaatcattc
    32461 ctgctaagtt ctcaggtgtc aattttgtct cagctaattc cagtttctca ctgttcaagt
    32521 aagcagaaac ttcacccatc aaccagttag atgcttgctt ggcatctgca ccattttcca
    32581 aaacagcttc aaaaaaatca gacatttctt ttgtaagtgt caataccatt gcgtcatact
    32641 ctggaagtcc aaactcacgg atatagcgtt cgcgacgaga agctggcatc tcaggcaaac
    32701 tagcttttac tttttgaatc cattcgtcat cgatcacgaa tttcgggata tcaggctctg
    32761 ggaagtaacg ataatcacta gacccttctt tgacacgcat caaaactgtt ttattcgttg
    32821 tttcgtcaaa acggcgtgtt tcttgttgga tttctcctcc tgaaagaaga acttttgctt
    32881 gacgtttttc ttcaaaagct aaaccttttt tcacgaaaga taaagagttc aagtttttca
    32941 attctgcttt cgtaccgaac tcttcttgtc cataagggcg aagagaaata ttggcatcac
    33001 agcgcataga gccttcttcc attttcacat cgctgacttc tgtaaattgg ataattgatc
    33061 gcaatgcttc tagataagcg tatgcttctt ctggtgaacg catatcagct tcagaaacga
    33121 tttcgatcaa aggcgtgcct tgtcggttca aatcaacgta agaataaccg ccaataccat
    33181 gcatgttttt tccagcatct tcttctaaat ggacacgttc gatccggatt tttttctttt
    33241 taccttctac ttcgatttcg atccatccgt catgaccgat tggttgatca aattgggaaa
    33301 tttgatatgc ttttggatta tctgggtaaa agtaattttt ccggtcaaaa tgagtatctt
    33361 tagagatttc acagtttaat gccaaagctg ctttcatccc gaattccagc gcagctttat
    33421 tcattactgg cagcacgcct ggataacccc agtcgatcac gtttgtattg ctgtttggtt
    33481 ctgcaccaaa atgagctggc gctggagaga aaatttttga gttggttttt aactcgacgt
    33541 ggacttctag tccaatgact gtttcaaaat tcattcgtct ttccctccta aaatagctgg
    33601 ttgttgttta tggaattctg ttgcttgttc aaacgcatat gccgcttgat acatcgtttt
    33661 ttcatcaaaa taattaccga tgatctgcaa tccaactggt aatccttctg ataaacctgc
    33721 cggtacagac atacctggaa gtccagccaa gttgacaggg atcgttaaga tgtcactcat
    33781 gtacattgtg attggatcgt taatgttctc accgattccg aatgcaactg ttggtgaaga
    33841 tggcccgatg atcaagtcat aatctgcaaa gactttttca aaatctcgtt tgatcaatgt
    33901 acggacttgt cctgcttttt tgaagtaagc gtcataataa cccgcgctta aggagaatgt
    33961 acctaacatg atacggcgtt ttacttcttc accgaatcct tctgaacgtg agttgacata
    34021 tacatcatct aatgatttca cattttcaga gcgataaccg tagcggatac catcgaatcg
    34081 ttgcaaattg gaacttgctt ctgatgaggc aatgatataa taaacagcca caccgtattt
    34141 tgaatgaggc aagctgactt cttccacaac agcacctaat gaacggaatg tttctgctgc
    34201 tttcaataca gcttctttta cgccaggggc cacaccttcg cctaagtatt ctttaggtaa
    34261 agcaatcttc attcctttga tgtcgcctgt cagtccctct gcaaaatcag gaactgaaac
    34321 gcctgaagaa gtaccgtctt tttcgtcata accgctgata gctgtcaatg ctaatgcatt
    34381 atctttcacc gtacgggtca ttggtccgat ttggtcaaga gatgaagcaa aggcaatcaa
    34441 accaaaacgt gagacacggc cataagtagg tttcatccct acgatgccgt taaaagctgc
    34501 tggttggcgg atacttcccc cagtatcact acctaaagaa actggaattt gtccagctgc
    34561 aactgctgct gcagaaccac cagaagagcc accaggtact tttgttgggt cccaagcatt
    34621 ttttgttttc ttgaaataag aagtttctgt acttccaccc atagcaaatt catccatgtt
    34681 tagcttacca acagggatca tatctgcttg atagatttta tccatgaccg ttgcatcata
    34741 aattggttca aaattgtaaa ggatcttaga tgcagcggtt gtcaagatat cttttgttac
    34801 gatattatcc ttgataccaa ttggaatacc agcaaggacg ttgctttcat caatcccttt
    34861 ttcatctaaa gcttttgcct gttccaatgc tttttcttcg ttcaatgtaa tgaatgcctc
    34921 gacctcaggc tctgtttctt tgattcgttc aaatgttgtt tttgtcaaat cttgtacagt
    34981 gatttctttc gatacaagca tatgatgcag ttcttctaga gaacgattgt gtaattctgt
    35041 cattatgcgc cagcctcccc attatcgatg attgcgggta ctttgataaa gccgtcttct
    35101 gtttccggca catttttcaa taattcatcg cggttccatc cagcttgcgc ccgatcttgt
    35161 cgcatcacgt ttactgtttc aaccacattc gacgtaaatg gaacaccttc cgtatctact
    35221 tcttccagca tttcaaccat atcgatgatt ttacctaatt ggtcggtaaa acttgcaagt
    35281 tcttcatctg cgaacgcaag ttttgctaat ttggcaacgt gtttcacttg ttcttcacta
    35341 atcgccatac taccccttct ttctaaacga gttaaaaact ttgctctcta ttttaacaca
    35401 agagtgccgt gtctgaaatt gtttattgaa atttgtctga aaaaactcat ttttcattct
    35461 tctactgagc gacttttagc caatgcatct gccatttgat cttctgtcca aacttcgatt
    35521 cctaattctt gtgctttggt cagcttgctg ccagcatctt ctcctgctac cacgatatcc
    35581 gtttttttcg atacgctacc agtcactttc ccgccaagat tttcgatcgt ctcttttgct
    35641 tcttcacgag taaatcgtgt tagtttacca gtaaggacta ctgttttttc tttaaaagga
    35701 gactcgactt cttgcaattg ggcatttcga agccctttgt attcgaaatt gacgcctgct
    35761 tgttttaatt cattcatcaa ttcatggact tcttcgttac tgaaataagt gactacactg
    35821 tcggcaatga tatcaccgat cgtatcaagc gcaataatcg actcatagtc acttttactc
    35881 aatgtttcta gatcaccgaa atgttctgct aaaatttttg ctgctttcgc tccgacatgt
    35941 cggattccta acccgaaaat caaccgctcg accgaattat ctttgctgtt gtcgattgct
    36001 gtcaaaatat tatttgctga tttttctttt attttgtcca acgtcaataa ctcttcttct
    36061 gtcaatttgt aaagatcagc aacatctgct actagttttt tatcatacat ctgttctagt
    36121 acgcgcggcc ccaacccatc gatattcatc gcatttcttg aaacaaagtg gttcaaacct
    36181 tctttcattt gagcagggca tttaggattg atgcaccgta aggctacttc ttcatccaaa
    36241 tggacaagtt cacttccaca aactggacaa tgtgtaggca attggtattc ttcgctgtct
    36301 ttaggtcgtt tatctaacac gacttgcgaa acttctggga tgatgtctcc cgctttataa
    36361 atcagaactg tatctttcaa gcggatatcc ttcaacttaa tataatcacc gttatgcagg
    36421 ctggcacggc ttactgtcgt tcctgctact cggactggtg tcatgatagc tgttggcgtt
    36481 actacccctg ttcgaccgac tgtccattcg atgttttcga ttagcgtttc aacttcttct
    36541 ggcggaaact tgtaagctgc tgcccatctc ggtgctttga tagtaaaacc aagttgatct
    36601 tgtagcgaga attcattcac tttgatgacg attccatcga tttcataggg caaatccaca
    36661 cgtttgtcat gatattcttc aatgtatgac cacacttcat caatcgaatg acatagacgt
    36721 ttttcccgat tcgtgtggaa tccaattttt tctagctctt ctagcgcatc atactgggtt
    36781 tttgcttgca taggaccgaa atcagctact gtgtataaaa aagtgtccag atttcttttc
    36841 gctgtgattt tagaatctaa ctgacgcaaa gaaccagctg cggcgttccg aggattcgca
    36901 aagatatctt ttccctctgc ttctctgtct tggttcaatt ggacaaatga ccgttttggc
    36961 atgaaacatt cgcctcttac ttcgatgttc attggttctt ttaatttgat tgggaccgac
    37021 cggacagttt ttaggttttc agtgatgttt tctcctacag ttccatcccc gcgagtcgca
    37081 cctctcacga aatttccatc ttcataacgc aaagaaacgg aaagtccatc tatttttagt
    37141 tcacagcaat agtcaactac acgtcccact gctttttgta cacgttgatc aaaagctatc
    37201 agctcttctt tgctgaaaac gtcgttcaaa ctgtaaagcg gaatatcatg agtgactttt
    37261 tcaaaacctt ctaatacttt tccgcctact cgttgagtgg gagaatcaga cgtgatcaaa
    37321 tcaggatatt gttcctcgat tgctactaat tctgcgtatt ctttatcata aacatagtct
    37381 tccactgttg gtttatcttc tacgtagtat tcgcgagacc attggttcag tcttgtacga
    37441 agttcttctg ctcgtttagc agcttcgtca atcgtcattt catgttccat catttaccct
    37501 ccactactaa gctttttcaa ttggcgcaaa ggcagccaaa agacgtttta ctccttgact
    37561 tgggaaagcc acatctagtt ctagatcctt cgacgtcccg cttactcgga cgactgttcc
    37621 tagaccccat tttttgtgtt tgactttatc tccaggtttc cagctgttat tttcccctcc
    37681 agtagcactt ttagagggaa caaccgctgt ctctggctgt ttatagctat atgctgtttt
    37741 tttagcgaat acaggcgttt ttttaggcgt aggacgcatg ccttcgattt ccagtaactc
    37801 ctgatcgatt tcttctacaa aacgagaagg tcggttatat tgcgtccgtc catataatgt
    37861 tctggagaaa gcatttgtta aatacaacgc ttcttctgct cgcgtgatcc ccacatatgc
    37921 gagacggcgt tcttcctcca gttcgctttc ttccatcaat gctcgagata aaggaaaaac
    37981 cccctcttct agaccaatca ggaatacaac tggaaattct aatcctttgg ctgcatgcaa
    38041 agtcatcaat gtcacttggg aagcatcttc ttctaaattg tcaatgtcgg aaacaagtgc
    38101 cagatcattt aagaagaccg ttagtttttc ttctggtgca tcagcgtctt cttcattttg
    38161 ctgttcgaat tgtttgtcga attcttgtgt gaccgttaaa aattcctcca agttttcgat
    38221 gcgtgcttgt gcttctaatg tattttggat ttttaagtct tctagatatc cgctacgatc
    38281 caaaacctct ttcgtcaatt ctgtcactgt caagtatggg atcatctgag tgacttcttg
    38341 gatcatctct ccaaatgcac ctaattgctg tcctgctttt ccaccgatat ttgccagatc
    38401 cacattttgc gctgcttcaa gcagtggcca ttcatgaaga gaagcaaaac tgcgtagttt
    38461 ttcgatagat cctggtccga tgcctcgttt gggcgaatta acgatacgct caaaactgat
    38521 tgaatcttga ggatttacca gcacattcaa atacgctaaa atatctttga tttctttacg
    38581 atcgtagaac ttgtgccctc caaccatttt ataaggaata tttgctttca acagagtttc
    38641 ttccattaca cgagattgtg cgttcgtccg gtacaagatg gcaaagtcgc cgtaattccg
    38701 atgattgctt cgaatctctt cttgcatccg gtctacgata aaacgggttt cgtctcgttc
    38761 attatctgca cggtagtaag tgatcttgtt tccttctttg ttttctgtcc acaagttttt
    38821 tggttttcga ttgctattgt tttcaatgac ttggtttgct gcgctaagga tgtttttcgt
    38881 ggagcgatag ttttgttcta gcaaaattac tgcagcatct ggataatctt tttcaaaatc
    38941 caagatgttc tgcatatctg caccgcgcca gccgtaaata ctttgatctg catctcccac
    39001 gacgcacagg ttcctaaatc tacctgccaa taaattgact agcgtatact gtgcgtgatt
    39061 tgtatcttgg tactcatcta catgaatata atggaatttg ttttgataat acgtgagtga
    39121 atcaggatgt tcttcaaata atcgaatcgt attcatgatc aggtcgtcaa agtccataca
    39181 ttgattatat tgaagctctt tttggtaagc agcataacat tttgccgcaa tctcttcgta
    39241 ataactgcct tgcatttttg caaaatcctg aggggtctgc aggctgtttt ttgcttgaga
    39301 gattgttcct aatatacttc gaggatcata ttttttcgga tcaatgttca gttcttttag
    39361 tatccgtttc atcaaggtca gctgttcaga agaatcgatg attgtaaagt tccggttata
    39421 cccgataaaa tcaacatctc ttcttaaaat acgcacacac atcgagtgaa aagtcgatac
    39481 ccagacatct tcgcctccag atgcgagaat cgcattgaca cgttctttca tttcttttgc
    39541 agccttattg gtaaaagtaa tcgctaggat gttccaagga ttgacttctt tttcctcaat
    39601 caaataggca atgcggtgtg tcagcactct cgtctttcca cttcctgcac ctgccattag
    39661 cagtaatggt ccgtcagtgt gaagaacagc ttctttctga cgggggttca tcccgtttaa
    39721 taattcagct ttggatgaca ttaacaacat cctttctttt atcaatcttg ttaattatag
    39781 cattttcttg gctttcatta aactagctag ttcaaaatca tttgtttctt tttatggatt
    39841 tttccttgtc aattcgttga tttcgcgcta aattaaaaga aagcgaggcg tttgaatgga
    39901 aaaaaaagga aaaatcaaga aattagataa gactcagata tattttattc taaaaggaat
    39961 atttgttgga ctgctgacag gaatcgtcgt tagcctgttt cgattatcta tcgaaaagct
    40021 aagcgattta gttcgaagta tctatgaaat gagtcgggaa cagccgattt acttaattgg
    40081 cctagctatt tgctgcatcc ttgcagcatt tttcgtgggt tatctggtca aaaatgaacc
    40141 ggatatcaag ggaagcggga ttccacaagt agagggacag cttcgcgggg aattatcaat
    40201 gaactggttc tctgttttat ggaaaaagtt cattggcggt gtactttcag ttggtgcggg
    40261 cctttttcta ggtagagaag gaccttctat acagcttggc gcatccattg gacaaggagc
    40321 tggacaattg tttcggagtc catcttcaga agaaaagatt ttaatttcta gcggtgcaag
    40381 tagcggactt gcagcagcat tcaatgctcc tatcgcagga ttactctttg ttttagaaga
    40441 aatccatcac tccttttctc cacttgtttg gctgacatca tttgcttcag caatcactgc
    40501 taacttcgtc tctctttact ttttcggctt gagacctgtt ttatatctag gtgaccttcc
    40561 ttctttgccg ttgaaatatt atggttcctt agtgatctta ggagtacttc taggcatctt
    40621 aggttttatt tatcagaatg tcttgctggc tttgccaaaa ttttacaacc gattgccttt
    40681 accagcttat tttcacgggt tcgttccttt cttgctgatt ttgccaattg gttatttatg
    40741 gcctagtact ctaggcggag gcaatcaaat cgttttagct tttggacatg cttccttgcc
    40801 aatcattatg attatcggtt tgtttgttct tcgattcgtt ttttccatga tttcttacgg
    40861 tgggggtctt ccagggggga ttttccttcc gatattgaca ttgggggctt taatcggtag
    40921 cttttatgga aacattatgg ttgatttcgg gatggatcca atctacatca aggattttgt
    40981 cgttattgcc atggctggtt atttcacagc gattggaaaa gcaccgctga cagcaattat
    41041 tttagtgaca gaaatggttg gctctcttaa tcatctgatg cctttaggat tggctgctct
    41101 tgttgcttat atcgtcaatg accttctcgg cggtaatcca atttacgaat cattattgga
    41161 acgtctactt ggaaatactc agaccgcctc actaaaaggt gcaaggacaa cttttgattt
    41221 tccagtaacg gcagagagtt ctttagacgg attaatggta cgagacttca attggcctaa
    41281 aaatatgctg ctaatctcta tacgcagagg aaatcaggaa atcttgactc acggtgatac
    41341 gatcatgaat gtaggagata ttttggttat cttgactgat gaagcaaact tgccaagaat
    41401 caagcaggaa atcgaggcta aatcttctat cacaaatttg aagcaatcat tttaacgaac
    41461 atggaaaaac tattttacaa caactattca tctacactac ttgattctat atgattctat
    41521 aaaaaaagaa gaggctggga aaacccagcc tcttcttttt tatttctatg cttctgctac
    41581 tgcaggagct tttctcaata aaccataaat cacaccagat acgactgctc cgatagcaat
    41641 aaacaccaaa tacatgaccg ggtgactcag taagaataca acgaaaatcc cgccgtgagg
    41701 agccagtaat ttgattccga aagctccgac taatccgcca gttaaagcag aaccaataat
    41761 aaaacttgga atggcacgaa tcgggtcagc tgctgcaaat ggaatcgatc cttccgtgac
    41821 aaacgatagt cccatcacga tgttcgtcaa accagcatct tgatctgttt tgctgaattt
    41881 attcttaaac aaacgtgtag caacgaaaat tgccaatgga ggtaccattc ctccagccat
    41941 aacagaagcc atcacgacac ttccgccagt tgcaacagtc gtagctaaag ttgctgtacc
    42001 aaacacataa gcggctttgt tgattggacc gcctaaatct gccgccatca ttccagctaa
    42061 taatgcacca agtaaagcag cattcgaacc actcatactt gtcaagaaac cgttcaatgc
    42121 atcattgatt gctttcatag ggacattgac cagcaacatc aagaaaccag taatcagtaa
    42181 gccaaacact ggataaaaga ggatcgtttt aattccatcc aatgattttg gtaagttctt
    42241 gaataattta cgtaaaaata cgatgacata accagctaag aatccaccaa tcagtgcacc
    42301 taagaatcct gcaccgcctg tattagctaa tgcaccagca gcaaatccaa cgatcaaacc
    42361 agggcggtca ccgatacttg aagcaataaa tcctgctaat accggcagca tgaaaccaaa
    42421 tgcagcttgt ccgatttggt tgaaccagct agcagcttga ttgtaattcc ctagatttga
    42481 taattgatca tgtggcacgc caataaattg gtcgatcatg aaagaaagcg caatagcgat
    42541 tcctccacca ataacgaaag gtaacatgtg tgatacgccg ttcatcaaat ctttgtaaat
    42601 tctttgtccg atagtaccat ccgcatttcc ttcttctgaa ctgctttctt ttccatcacc
    42661 atggaatact ggtgcagtac cactaagggc tagattgatc aactcttctg gtttacggat
    42721 accatcactg acaggacgat tgatcagttc ttttccgtca aagcgattca tctctacttt
    42781 tttgtctgct gcaacgatta caccttctgc acgggcgatg tcttcagcag tcaatcggtt
    42841 tttgattcct tctgaaccgt tcgtttcgac tttgatctcc acacccattt ctttcgcttt
    42901 tttcttcaaa gcatcttctg ccatgtaagt gtgagcgatc cctgttggac aagctgtaac
    42961 ggcaacaacg aattttcttt ctggatgagc agaagcttct gcagcttgtg tttcttgttc
    43021 tgctttttct tctgcttctt tttgctcttc agctgtttgg aacaagtctt ggacttcttc
    43081 tggtgtattt gcttctttta attttcctac gaaatctgca tcaatcagca atcgagatag
    43141 tgctgctaat gcttgcaaat gtgtgtcgtt tgcgccttct ggagctgcga tcatgaaaaa
    43201 cagataagtt ggctgtccgt ctaaggcttc ataatctact cctttttggc tttttgcgaa
    43261 taaaactgtt gcttctttaa ctgcactgtt tttcgcgtga ggcatggcga ttccatcacc
    43321 aagacctgta gaagtttgtg cttcgcgggc tagaattcct tctttatatg tttcgatatc
    43381 ggaaatccgg ccggcatcaa acattttttg gaccatttca tcgattgcgc cttttttatc
    43441 agtcgcttgc aaatccatga tcatcgcttc tttgaccatt aaatccttga tattcatgat
    43501 tatcctcctt gaaactaatc aatttttgtg atttcaactt ctggcagcaa ctcatcaatc
    43561 aattcgcgag ctgccaagtc atcagaaaaa gctgttgcgc ttccgcaagc aactccccat
    43621 ttaaaggctt cgattggatc tttcgtacgg ctgtaagccc caataaagcc agcaatcatt
    43681 gaatcccctg cacctactga atttttcaat gggcgaacta atacattcgt tttataagtt
    43741 ccttgttccg tgaagagcaa agcaccgtct ccagccattg agatcagcac attctgtgcc
    43801 ccttcagcta gtaatttttt cccatacgga atgatttctt ctacagattg gaaggttgtg
    43861 ttgtaaagct cagctaattc gtgattgttt ggtttgatca acaacggttt cttaacaagt
    43921 gcatttttta ggtcatcacc tgttgtatca atcacgaaat ctgcaccttt ttctcgaatg
    43981 atatcaatca gttcttgata aaatcctttt cgcaggctag ctggcgcgct tcctgacaat
    44041 acgacgatat ctccttgtcc tactgttcct aatgcttgct tcaactcatc gatttcttca
    44101 tctgaaatag atgggcctaa gccattgatc tcagtttcag tgtctgattt tagtttgata
    44161 ttgattcttg tatcttcttt tacggttgtg aacaaagact ggatttgttc tttttcaagc
    44221 cacttcgaaa taaattcgcc agtgaaccct cctaaaaacc ctagtgctgt agattgagaa
    44281 tcgatccgtt tcaaaatacg ggaaacattg atcccttttc ctccaggtaa cttgaagtct
    44341 tccttcattc ggttcaaatc gcctagtttt aacccatcta cgcgtacgat ataatcaatt
    44401 gatggattaa gtgttactgt gtaaatcatt gtattgcctc cttaattgtt gtcttttcac
    44461 ggaattcatt aacgaattct aatggacaac gctcggttat gatcgtggct gcttccaaag
    44521 gtgcaacaga ggtgaaggac aactggttga atttggtatg atcaatcaat acaaatgtct
    44581 cttcggaatt tttcatggct aaacgtttaa cagctgcctc ttctggatcc ggagtcgtga
    44641 atccttgttc aagatgggca ccgttcattc ccataaatgc tttattaaaa tgaaaatttt
    44701 ttagttgttc tatcgcatta gatcctaaaa cagcattagt tgccagcttt atcgttccac
    44761 ctaaaataat ggtagcaagc tccaaatcaa ctaaatttgc cgcatgttga acggaatttg
    44821 tcactactgt gatctgtttt ccttgcagaa aaggaatcat ttctaacgta gtagagccag
    44881 cgtctaaata aatgacatct tcgtcattaa tggtttcagc tgccgatttg gcgatttgct
    44941 gtttttcgtg aacgtttttg agtgattttt cactcatatt ctgctcaaaa ttcaataatt
    45001 gctctttctt agctccgcca tgaactcttt ctagaaaccc actattctcc agctcttgta
    45061 aatcacgtct tatcgttgat tcagaagcat tcaaaaggtt tacaagatct tgtgatttca
    45121 cgattttctg ctgttcaagc aagtctagga ttttttgtct gcgttcctct gtaagcattt
    45181 tcacgatcac ctcaatacaa ataatagcat attttttttc tactagcaat ataaatcagt
    45241 caaaaacgtt cataaaaa
//
LOCUS       DACDSR010000014        45086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDSR010000014 DACDSR010000000
VERSION     DACDSR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.014"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWK15_000795"
     CDS             212..604
                     /locus_tag="IWK15_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9546417.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWK15_000796"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWK15_000796"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9546418.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWK15_000797"
     CDS             3165..4775
                     /locus_tag="IWK15_000797"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9546419.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWK15_000798"
     CDS             5067..5936
                     /locus_tag="IWK15_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9546420.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWK15_000799"
     CDS             6101..7372
                     /locus_tag="IWK15_000799"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9546421.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWK15_000800"
     CDS             7369..8664
                     /locus_tag="IWK15_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9546422.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWK15_000801"
     CDS             8763..9026
                     /locus_tag="IWK15_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9546423.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWK15_000802"
     CDS             9471..9920
                     /locus_tag="IWK15_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9546424.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWK15_000803"
     CDS             9977..11395
                     /locus_tag="IWK15_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9546425.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWK15_000804"
     CDS             11395..12255
                     /locus_tag="IWK15_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9546426.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWK15_000805"
     CDS             12429..13202
                     /locus_tag="IWK15_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9546427.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWK15_000806"
     CDS             13349..13714
                     /locus_tag="IWK15_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9546428.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWK15_000807"
     CDS             complement(13862..15070)
                     /locus_tag="IWK15_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9546429.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWK15_000808"
     CDS             complement(15089..15367)
                     /locus_tag="IWK15_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546430.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWK15_000809"
     CDS             15560..16216
                     /locus_tag="IWK15_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9546431.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWK15_000810"
     CDS             complement(16658..17455)
                     /locus_tag="IWK15_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9546432.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWK15_000811"
     CDS             complement(17452..18636)
                     /locus_tag="IWK15_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9546433.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWK15_000812"
     CDS             complement(18661..19908)
                     /locus_tag="IWK15_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9546434.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWK15_000813"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWK15_000813"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9546435.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWK15_000814"
     CDS             21888..22202
                     /locus_tag="IWK15_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9546436.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWK15_000815"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWK15_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9546437.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWK15_000816"
     CDS             22887..23531
                     /locus_tag="IWK15_000816"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9546438.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWK15_000817"
     CDS             23546..23875
                     /locus_tag="IWK15_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546439.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWK15_000818"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWK15_000818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9546440.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWK15_000819"
     CDS             24863..25687
                     /locus_tag="IWK15_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9546441.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWK15_000820"
     CDS             25680..26027
                     /locus_tag="IWK15_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9546442.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWK15_000821"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWK15_000821"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9546443.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWK15_000822"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWK15_000822"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9546444.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWK15_000823"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWK15_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9546445.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWK15_000824"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWK15_000824"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9546446.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWK15_000825"
     CDS             31684..32451
                     /locus_tag="IWK15_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546447.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWK15_000826"
     CDS             32441..34483
                     /locus_tag="IWK15_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546448.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWK15_000827"
     CDS             34764..34913
                     /locus_tag="IWK15_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9546449.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWK15_000828"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWK15_000828"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9546450.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWK15_000829"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWK15_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9546451.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWK15_000830"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWK15_000830"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9546452.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWK15_000831"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWK15_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9546453.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWK15_000832"
     CDS             39330..40412
                     /locus_tag="IWK15_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9546454.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWK15_000833"
     CDS             40412..41215
                     /locus_tag="IWK15_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9546455.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWK15_000834"
     CDS             41285..42025
                     /locus_tag="IWK15_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9546456.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWK15_000835"
     CDS             42036..43040
                     /locus_tag="IWK15_000835"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9546457.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IWK15_000836"
     CDS             complement(43085..44929)
                     /locus_tag="IWK15_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546458.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14522 a         7846 c         9369 g        13349 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaa
//
LOCUS       DACDSR010000015        43701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDSR010000015 DACDSR010000000
VERSION     DACDSR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.015"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(341..2722)
                     /locus_tag="IWK15_000837"
     CDS             complement(341..2722)
                     /locus_tag="IWK15_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9546459.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2712..3359)
                     /gene="recU"
                     /locus_tag="IWK15_000838"
     CDS             complement(2712..3359)
                     /gene="recU"
                     /locus_tag="IWK15_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9546460.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3423..3962
                     /locus_tag="IWK15_000839"
     CDS             3423..3962
                     /locus_tag="IWK15_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9546461.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4052..4471
                     /gene="gpsB"
                     /locus_tag="IWK15_000840"
     CDS             4052..4471
                     /gene="gpsB"
                     /locus_tag="IWK15_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9546462.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4493..4860
                     /gene="rnpB"
                     /locus_tag="IWK15_000841"
     ncRNA           4493..4860
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWK15_000841"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5069..6235
                     /locus_tag="IWK15_000842"
     CDS             5069..6235
                     /locus_tag="IWK15_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9546463.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6281..7777
                     /locus_tag="IWK15_000843"
     CDS             6281..7777
                     /locus_tag="IWK15_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9546464.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7858..8541
                     /locus_tag="IWK15_000844"
     CDS             7858..8541
                     /locus_tag="IWK15_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9546465.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9186..10376
                     /locus_tag="IWK15_000845"
     CDS             9186..10376
                     /locus_tag="IWK15_000845"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9546466.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10478..11431
                     /locus_tag="IWK15_000846"
     CDS             10478..11431
                     /locus_tag="IWK15_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9546467.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11500..12849
                     /locus_tag="IWK15_000847"
     CDS             11500..12849
                     /locus_tag="IWK15_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9546468.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13032..13355)
                     /locus_tag="IWK15_000848"
     CDS             complement(13032..13355)
                     /locus_tag="IWK15_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546469.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13484..14647
                     /locus_tag="IWK15_000849"
     CDS             13484..14647
                     /locus_tag="IWK15_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546470.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14717..16066
                     /locus_tag="IWK15_000850"
     CDS             14717..16066
                     /locus_tag="IWK15_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9546471.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16111..16542)
                     /locus_tag="IWK15_000851"
     CDS             complement(16111..16542)
                     /locus_tag="IWK15_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546472.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16930..19572
                     /gene="alaS"
                     /locus_tag="IWK15_000852"
     CDS             16930..19572
                     /gene="alaS"
                     /locus_tag="IWK15_000852"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9546473.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19772..20368
                     /locus_tag="IWK15_000853"
     CDS             19772..20368
                     /locus_tag="IWK15_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9546474.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20490..21638
                     /locus_tag="IWK15_000854"
     CDS             20490..21638
                     /locus_tag="IWK15_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9546475.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21676..22089)
                     /locus_tag="IWK15_000855"
     CDS             complement(21676..22089)
                     /locus_tag="IWK15_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9546476.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22168..22599
                     /locus_tag="IWK15_000856"
     CDS             22168..22599
                     /locus_tag="IWK15_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9546477.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22698..22931)
                     /locus_tag="IWK15_000857"
     CDS             complement(22698..22931)
                     /locus_tag="IWK15_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9546478.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23114..24784
                     /locus_tag="IWK15_000858"
     CDS             23114..24784
                     /locus_tag="IWK15_000858"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9546479.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24931..25317
                     /locus_tag="IWK15_000859"
     CDS             24931..25317
                     /locus_tag="IWK15_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546480.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25304..26002
                     /locus_tag="IWK15_000860"
     CDS             25304..26002
                     /locus_tag="IWK15_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9546481.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26007..26471
                     /locus_tag="IWK15_000861"
     CDS             26007..26471
                     /locus_tag="IWK15_000861"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9546482.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26468..27379
                     /locus_tag="IWK15_000862"
     CDS             26468..27379
                     /locus_tag="IWK15_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9546483.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27614..28153
                     /gene="pyrR"
                     /locus_tag="IWK15_000863"
     CDS             27614..28153
                     /gene="pyrR"
                     /locus_tag="IWK15_000863"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9546484.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28167..29450
                     /locus_tag="IWK15_000864"
     CDS             28167..29450
                     /locus_tag="IWK15_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9546485.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29463..30389
                     /locus_tag="IWK15_000865"
     CDS             29463..30389
                     /locus_tag="IWK15_000865"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9546486.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30408..31688
                     /locus_tag="IWK15_000866"
     CDS             30408..31688
                     /locus_tag="IWK15_000866"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9546487.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31693..32769
                     /gene="carA"
                     /locus_tag="IWK15_000867"
     CDS             31693..32769
                     /gene="carA"
                     /locus_tag="IWK15_000867"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9546488.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32774..35959
                     /gene="carB"
                     /locus_tag="IWK15_000868"
     CDS             32774..35959
                     /gene="carB"
                     /locus_tag="IWK15_000868"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9546489.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36013..36789
                     /locus_tag="IWK15_000869"
     CDS             36013..36789
                     /locus_tag="IWK15_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9546490.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36786..37718
                     /locus_tag="IWK15_000870"
     CDS             36786..37718
                     /locus_tag="IWK15_000870"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9546491.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37715..38431
                     /gene="pyrF"
                     /locus_tag="IWK15_000871"
     CDS             37715..38431
                     /gene="pyrF"
                     /locus_tag="IWK15_000871"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9546492.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38438..39127
                     /locus_tag="IWK15_000872"
     CDS             38438..39127
                     /locus_tag="IWK15_000872"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9546493.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39100..39369
                     /locus_tag="IWK15_000873"
     CDS             39100..39369
                     /locus_tag="IWK15_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546494.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39407..40048
                     /locus_tag="IWK15_000874"
     CDS             39407..40048
                     /locus_tag="IWK15_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9546495.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40079..40783
                     /locus_tag="IWK15_000875"
     CDS             40079..40783
                     /locus_tag="IWK15_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9546496.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40780..41682
                     /locus_tag="IWK15_000876"
     CDS             40780..41682
                     /locus_tag="IWK15_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9546497.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41840..43546)
                     /locus_tag="IWK15_000877"
     CDS             complement(41840..43546)
                     /locus_tag="IWK15_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9546498.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14004 a         7700 c         9490 g        12507 t
ORIGIN      
        1 taactcaaaa tgaagccttc tttcaaaaaa ttattcaaaa ccatatattg tattttttat
       61 ttgataataa caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta
      121 gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg
      181 attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa
      241 aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca
      301 caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg
      361 aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac
      421 tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag
      481 gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact
      541 cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg
      601 agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat
      661 tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc
      721 ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat
      781 aaacatcaga ggctacgtgt gacgattctg aagtgacagg tgtcattttt ttattgtaac
      841 ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca
      901 ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct
      961 taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat
     1021 cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg
     1081 gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt
     1141 aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc
     1201 cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg
     1261 tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat
     1321 tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg
     1381 tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg
     1441 gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat
     1501 ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc
     1561 ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat
     1621 tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat
     1681 ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct
     1741 ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt
     1801 tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat
     1861 ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa
     1921 gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat
     1981 cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt
     2041 ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg
     2101 acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca
     2161 atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg
     2221 tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc
     2281 cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt
     2341 tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat
     2401 tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg
     2461 tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt
     2521 ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta
     2581 ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac
     2641 ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc
     2701 ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag
     2761 aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct
     2821 agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag
     2881 taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac
     2941 aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca
     3001 tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag
     3061 taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc
     3121 ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc
     3181 tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct
     3241 tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt
     3301 ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc
     3361 tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg
     3421 agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt
     3481 ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc
     3541 agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat
     3601 gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt
     3661 tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat
     3721 tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta
     3781 gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct
     3841 atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag
     3901 cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat
     3961 aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt
     4021 tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga
     4081 cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga
     4141 attcttagac aatatcatca aagattatga agcttacagc aaagaactcc ttgctttaca
     4201 agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc
     4261 tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat
     4321 cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc
     4381 aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt
     4441 agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg
     4501 gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc
     4561 ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga
     4621 aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg
     4681 gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg
     4741 gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag
     4801 atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa
     4861 aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa
     4921 aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt
     4981 ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct
     5041 ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc
     5101 aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga
     5161 atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc
     5221 taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta
     5281 tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact
     5341 agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga
     5401 ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc
     5461 agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa
     5521 agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg
     5581 cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac
     5641 aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat
     5701 tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga
     5761 atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa
     5821 agcagattat gatattgaat tagatattac tggttcagat atcaatggac ggatgattga
     5881 aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca
     5941 agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta
     6001 cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt
     6061 attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga
     6121 attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga
     6181 tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta
     6241 ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt
     6301 acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac
     6361 acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta
     6421 cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc
     6481 tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga
     6541 gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc
     6601 ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca
     6661 cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga
     6721 aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct
     6781 tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga
     6841 aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa
     6901 gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga
     6961 tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg
     7021 atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg
     7081 tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc
     7141 aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag
     7201 cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat
     7261 tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga
     7321 ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat
     7381 tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga
     7441 gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc
     7501 aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt
     7561 acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc
     7621 tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa
     7681 tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat
     7741 gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg
     7801 aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg
     7861 gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga
     7921 catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa
     7981 ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat
     8041 aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg
     8101 ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct
     8161 aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat
     8221 cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat
     8281 gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac
     8341 cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa
     8401 atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc
     8461 ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg
     8521 atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc
     8581 ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc
     8641 cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata
     8701 ttgtaacttg tgattcggaa ttggcaggag aacaccgttt attcggaaga ataggactgt
     8761 gacaagactt ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt taagaaatat
     8821 tcagagagta aaattaaaaa ttgctgataa acgaagatgg aaattttatg tatttttatt
     8881 ggtctatgca ataaataaat taaatattca gaatacaatt gattttaacg aaagggcttc
     8941 tatcaatggt tgtgttgtat ttatctgccc aggtagagta aaatagatca ttatctaaaa
     9001 ggtaattgtt catactatat aagtcatttg aaagtaaaaa gatactgtaa ataaaaaatg
     9061 tggttaaata attctcataa tcagtgtaag cgttatcaaa aatatagcat acttgctttg
     9121 tagatgaatg acacgatata tgtttggtat gttctgcaag acagatgaat atggaggtgg
     9181 agacaatgtc ttccaaagaa ctaacaatgt atttagagaa agaattggat caactgaaag
     9241 aaaaggggtt atacaatacg atcgacatac tagaaagtga aaatggtgct tgtatcatcg
     9301 tagacggaaa aaagatgatc aatctagcat ccaacaatta tcttggattt gccaatcgcg
     9361 aagaattgaa aaaagcatgt attgaagcaa ccgaaactta tggtgtagga gcaggagcag
     9421 tccgaacgat caatggttcg ctgaaaatcc atcagcaatt agaagaaaaa atcgcagaat
     9481 tcaaagggac agaagcagca atcgcctttc aatctggatt caactgcaat atgggcgcta
     9541 tctctgcagt aatgacaaaa gaagatgcta tcttatcgga tgaattaaac catgcatcta
     9601 ttattgacgg ttgccgctta tccggagcaa aaatcatccg aattaaacat caagatatga
     9661 aagaccttga gaaaaaagca aaggaagcga tagaaagtaa gaaatataaa aaaatcatgt
     9721 atatcacaga tggtgttttc tccatggatg gagacattgc acgattgccg gaaattattc
     9781 cgattgttga aaaatatgga ctgatcacct acgtcgatga tgcgcacggc tcaggcgtaa
     9841 ctggaaaagg tgcaggaacc gtcaagcatt ttggattaag cgacaaaatc gatatgcaaa
     9901 tgggcacgtt atcgaaagct ataggtgttg ttgggggtta tgtagctgga tcgaaaacac
     9961 taatcgactg gttgaaagct agaagtcggc cattcctatt ttctacttcg ctaactccag
    10021 gtgcagctgc agctgcattg gcttcgatta ctttgatgca agaacatccg gaacttgtcg
    10081 aaaaagtttg ggaaaatgct aattatttta aagaagaatt gaaaaaagta ggctataaca
    10141 tcggtatgtc tgaaacacct attactccag tcattttggg agacgaaaaa gtgactcaga
    10201 ctttctctaa gaagctgatc gaacatggga tttatgctaa accaatcgtt tatccaactg
    10261 tgcctcttgg gacaggcagg atcagaaata tgccgactgc ggaacatacg agggaaatgc
    10321 ttgatgaagc agtagcagtt tatcaaaaaa taggaaaaga aatggaaatt atttaactaa
    10381 atttagtttt ttcgtctatt taaaattaga gaacatgttc acgcatatat tgttaatcat
    10441 ctgaagatag tagtaaaaaa gatcagggag gatcatcatg aaaaaagtat tggtgacagg
    10501 atgtttagga caaattggtt cagagttgat cacacggcta cgtcaagaac taggcagtga
    10561 aaatgtcatt gcgacagata tacgcatgcc tgaagtcatt gagaaaaacg atatctttga
    10621 gatcctagat gtaatggatt acgaaaaaat gcgcagcata gcagaacaat atcaagtgga
    10681 cacattgatt catttggctg ctcttttgtc agctgttgca gaagcaaaac cacaattagc
    10741 ctggaatttg aacgtgactg ggctggtcaa tgcattggaa gtcgctagag aattccagct
    10801 gaaatttttt acgccaagtt caatcggcgc gtttggtcca aatactccaa aagatcatac
    10861 cccacaagat actcttcagc gtcctacaac catgtatggg gtaacgaagg tagctggaga
    10921 actactatgt gattattatt atgtgaaata tggcgtagat actcgtggtg tccgtttccc
    10981 tggattgatt tcttatgcaa ccttgccagg aggcggtacg acagactacg cagtcgatat
    11041 ttattattca gcaatcaaag aaggaaagta cactagtttc attgatcaag gtacatcaat
    11101 ggatatgatg tatatgccag atgcgatcga tgccattata aaactcatga atgcagatcc
    11161 tgatcgattg gttcatcgaa atgccttcaa tatatcggcg atgtcttttg acccggaaga
    11221 gatcaaagca tctattcaaa aaatcatacc agattttgaa atggattatg atgtggatcc
    11281 tgtacgtcag tcaatagcag aatcttggcc aaattcacta gatgtttctt gtgcgcaaga
    11341 ggaatggggc ttttcgccga aatacgactt agatgtgatg acaaaggata tgttggaaaa
    11401 actgaaagaa aaattggtag agacggtata aaacaagaac cacatccaaa aagtttttta
    11461 tacttttggg attggctatg gtatgaaagg ggacaaggga tggaagaata tcaagccacg
    11521 ccagtaaaag aagtaaaaat agaaaatgaa ttaaaacgta cgatgggatt ttttactgct
    11581 ttatcgactg ttatgggcac agtgatcggt gcaggggtat tttttaaagc cgcaagcgta
    11641 gcagaagtta caggatccgc cagtttgcat atgttttctt ggtttctcgg tggtatgatt
    11701 tctgtctgtg caggactaac aggagctgaa ctggcagctg ctattccaga aacaggaggg
    11761 atgattaaat atatcgaacg gatttacgga aatacagcag cttttttatt gggctgggcg
    11821 caagtcgtga tttattttcc agcaaatgta gcagccttgt cgatcatttt cggtacacag
    11881 ttcgtcaatc tatttggtct ttcccagtca atgattgttc ccgtagctgt tacagctgct
    11941 gtatcgatcc tgttgatcaa ttttttaggt tctaaagctg gaggggcttt tcagtcgatt
    12001 acgcttgtct gcaagcttat tcctttgttt gtgatcgtca ttttcggttt gttccgacaa
    12061 gaaggagtag acttccaact gtttccgatc caagcaggag aaaatctttc atttttctca
    12121 gccttgggag cgggcttgct agcaacgatg ttcgcatacg acggttggat acacgtagga
    12181 aatatttcgg gagaattgaa aaagccggcg aaagatttgc caaaagcaat ttctttagga
    12241 atcatcggaa tcatgatcgt ttatttatta gtaaatgcgg tgttcttaag aaccgcatct
    12301 atcgacggag tcgcaggaaa cagcaatgcg gcaagtgacg ttgcaaaaat gatttttggc
    12361 ggcttcggtg gaagactggt cacagttggt atcttgattt ctgtttatgg aaccatcaat
    12421 ggctataccc ttactggaat gcgtctccct tatgttatgg caaaagaaaa caacctgcct
    12481 tttagcaagc tctttgctaa acttcatgat aaaactaaag ttcctgtagc cgcaggtatc
    12541 ttagagctag tcatcgctat tggcatgatg atgatcggtg gttttgatac actaacggat
    12601 atgctgatct ttgtcatttg gattttttat acgatggtct ttgttggtgt cattcttctt
    12661 cgtaaaaaag aacctgattt gtttcgacca tacaaagttc caatgtatcc gtttattcca
    12721 ctagtagcaa tcattggagg gacttttatc gtatcaagca cgcttatcac tcagaccttt
    12781 ttagcttcga tggggatagt attgacactt gctggtatcc cgatttatct ctatttaaaa
    12841 cgtagatagc taaaacattc gaattttatt agaactgcac ttctctgcca aacaaaatat
    12901 tttatttaaa tgaggagtac gtggaagaaa tgtcaaaagt actcgttaat tttgatcacg
    12961 taaatgtcgt tttgtctacg atgaaatagc aaagtaaaag acagaaatcg ggctcaaccg
    13021 atttctgtct tttactttga tttgcgtaac tgaactattc ggcgtacgcc gcgataaata
    13081 tttaataaaa ccaagcaacc agaaatgaaa atgggaagcc aaatcagata gacagggtag
    13141 ccaagcactt tagacaaagc atgtggagtc attttatcca tttcaagaag tgcttgctgc
    13201 caagtctgaa gattgaagac tagcatgaaa cccgaaataa tcaggagaat aaatcctaaa
    13261 taatgaattg tagttctttt tcttacataa gcgatcgtag cataaaggaa cagtaaaaaa
    13321 ataggtacgc caaaaacaaa gtacatattc agcatctcga cacccccttt gtgtttatct
    13381 tactttactt tgaaagaaat gagaaataat agcctcgtta cttgcttgaa acagcaaagg
    13441 actgctaaga taagagagat acaaagaatc aaggaggagt catatggcaa gttatatcga
    13501 accgattcgt tttgcgctga ttgtttttcc atttttagcc ttagcgatct catctgtttt
    13561 ttttatttat gaatatcgaa agtatggaac atttctagta acaagggcag ttattttgta
    13621 ttctttcgtt ttttatttat tgtgtgcgta ttttttggtg atcttaccac ttccggcaaa
    13681 ggagacggtt gcccaaatga cagggccaaa gatggagctg catttatttg cttcatggca
    13741 acatttcaga gacaacacgg tattggtctt gtctgaacct agtacctacc tgccggcaat
    13801 gaagcagagt gtatttttgg aaccagcttt taatgtttta ttactcatac catttggcat
    13861 ttatttaaga tattatttcc ggctttcttt tatgaaaacg atgattgcta gtttctgcct
    13921 ttctctattt tttgaattga cgcagctaag tggactttat tttatctacc caagaccata
    13981 ccgcttattt gatgtgaatg atttagtgac aaatacattg ggtggaatca ttggttttgt
    14041 cgttacgccg ttatttacat ttatgcttcc tacaagaaga cgaatggatg aactttctta
    14101 tgaaaaagga aagactgtct cattgactcg acgcttggtc gcttatttga tcgactggac
    14161 atttttatct cttattaatt tggtagcggc gattttcttt aaaattgcgt tcgattcagg
    14221 ggcagttact tctcatatct ggtggctttt attagaagta ggggtatact ttatgattgt
    14281 accgtttata accaatggat ggacacttgg caaaaaaatt gtgcgtatcc gtttggtgga
    14341 agaagaccgc gaacgaattt ctttaaaagc attgatgatc cgctacgggt atctgtactt
    14401 tcttttttat ggtttaagct ggttggggca gttgcttcag caaatcatga attcgacaac
    14461 gaatacggtg ttgcagatga gtgcagtatt catttctctc ggtgtcttgt tactacaagg
    14521 tatttttatt ttaaatatct tgctatcatt tttgagaaaa aaacggattc tgttttatga
    14581 aaaagctagt cacacatata ctgttagcac gattggccaa acaaaggatg atgattctcg
    14641 aaattagctg agcaaaaacg ctttgtctgt gttataatgc caaagtcgtg ataatttaaa
    14701 gagaagaggt gcacgaatga ataattttga tatattccgc ttcaaaccgt ttatcaatga
    14761 agcgttaacc gcaaagggct tcaaacagcc cacagaggtc caggaacgtt tgattccttt
    14821 aattgcaaaa ggaaaaaata tcattgggca atctcaaact ggtacaggaa aaacacatac
    14881 gtttttgttg cctttgtttg aaaaaatcaa tgcagaaaaa gaggaagtac aagttgttat
    14941 tacagctcca agtagggaac tagctgcaca gatctatgaa gcagctacac aaatcgctaa
    15001 attttctaca ccggagatcc gtgtctcgaa ttttgtcggc ggtacagaca aacagcgtca
    15061 attaaatcgt ctgcatcacc aacagccaca ggttgtagta ggcacgcctg gtagaatatt
    15121 agacatgatg aatgaacaag cgctgaagat tcacaccgca tttgcatttg ttgtcgatga
    15181 agcggatatg acattggata tgggtttttt aaatgaagta gaccaaattg caagtcgatt
    15241 accagaaaag ctgcagtttt tagttttttc tgccactatt ccagaaaaac ttcgtccatt
    15301 cttacgaaaa tacatggaaa atccaatcat tgaagaaatc aaaccaaaag cagttatttc
    15361 tgaagacatt gaaaattggc tgatttcaac aaaaggtaaa gatgagaatc aaatgattta
    15421 tcaactgctg acaattggtc atccttattt agcaatcgta tttgccaata caaaacagca
    15481 tgtggatgaa attgccgatt acttgaaaaa ccaaggcttg aaagtagcaa agattcatgg
    15541 agacatcacc ccaagagaga gaaaacgagt gatgcgacaa gtacagaatc tggagtatca
    15601 atatgttgta gcgactgatt tagctgctag agggatcgat atcgaaggtg tctctcatgt
    15661 tattaatgca gaaattccag aagatctaga cttctttatc catcgtgtcg gacgtactgg
    15721 acgtaatggc ttaaaaggaa cggccattac gttatattcg ccaaatgatg agcaagccat
    15781 tgatgcgata gaaaaattgg gcgtctcatt tgaaccaaaa gaaatcaaaa acggggaaat
    15841 cgtcaaaaca tacgatcgta atagacgtgc caaacgagaa aaatcaaaag atgtattaga
    15901 tcccacgctg attggtcttg tgaagaagaa aaagaaaaag atcaagcctg gttataagaa
    15961 aaaaatcgat tgggcaattg cagaaaaaaa taaaaaagaa cgcaaaatcg aacgcagaca
    16021 gcaaagccgt actgctcgta aaaataagaa aaattcacat aaataaaaaa caagaggttg
    16081 tgacaaaagg cgtgtcacaa tctcttgttt ttaagctaga ctttttttca ttccgataca
    16141 aagtacttca tcttcaataa aaggttctgt gcaagtatga tacccttgtt tttcataaaa
    16201 atctacagcc gtcatttcag ctactacata actagtctga caaccttctg aaaccgcccg
    16261 tttttcagca gcttcaagca actgaccgcc taaccctttt cctcggtatt cttttgctac
    16321 gcaaaatcgt tccggcttga agcaagtagg tgttttcttt tcataacgca aagtagctac
    16381 tggtcttttt tgatcaagaa atagataata ggtttgttgc gggatatctt ttccgtcgaa
    16441 ttctagttct gcaggaatcc cttgttctaa tacaaaaact tgatatctca aaaagcagct
    16501 agctgcgcgt atccattcat ccgctccaac tatttttttc atcatattcc tccttgtcat
    16561 gaatagcaga gatgtattga ttcctttatt cttatcgtac caaaaatcgt ggagtatgac
    16621 caaaaggaaa tgaaaataaa aattgagata ttgacagtga tcaatagaat gcgtatacta
    16681 attaagtacg aaaaggatat gatttgtttt ttaagactca cagaaaattc tttcttggct
    16741 gaaaaaagaa tagtcactaa tcaaattgct ccctttttct attttggtag aaataccgac
    16801 gcagctcagc gttatcgagt ttaagaggta atgattacac atcattgcaa tcaaggtggt
    16861 accgcgagtt ttcgtccttg gctgtgatgg tgtttttttg tatttaattt atagaaagca
    16921 ggtaatgaca tgaaacaatt aagtagtgca caagttcgac aaatgtttct tgatttcttc
    16981 caatctaaag gacatacgat tgaacctagt gcgtctttga tccccgtgaa cgatccaacc
    17041 ttattgtgga tcaactctgg tgttgcaaca ttgaaaaaat attttgatgg ttcagttgtg
    17101 ccagaaaatc caagaatcac aaatgcccaa aaatctattc gaacaaatga tatcgaaaat
    17161 gtcggtaaaa cagcacggca ccatacgatg tttgagatgc tgggtaattt ttcgattggt
    17221 gactatttca aaaatgaagc catccactgg gcatgggaat ttttgactga tccaaaatgg
    17281 atggcattcg atccagaaaa actttatgta acagtttatc caaaagatac agaagcaaaa
    17341 cgcatctggc atgaagaagt tggcctgcca ctagatcaca ttattgatgt agaagacaac
    17401 ttctgggata tcggtgcagg cccatgtggt ccggatacgg aaattttcta tgaccgtgga
    17461 gaagcttata acgatttagc agaagacgat cctgaaaact atccaggagg agaaaatgaa
    17521 cgttatttag aaatctggaa cttagtattt tcagaattca accataagcc tgataacaca
    17581 tttgaaccac ttcctcataa aaacattgat acggggatgg gattggaacg catggtctca
    17641 atcgttcaag atgcgtcaac gaactttgag acagacttgt ttatgcctat tatccacgcc
    17701 gtagaaaagc taagcaaaca agtaaaatat ggtgaaaacc cagtaacaga tatttcttac
    17761 aaagtaatcg cagatcatat tcgcgccctt tcatttgcta ttggcgacgg tgcattgcca
    17821 tcgaatgaag ggagagggta tgtcttacgc cgtttgcttc gtcgtgctgt catgcatggg
    17881 aaaaaattag gtattgaaca agcattctta tatcgtttag tccctgttgt tggggaaatc
    17941 atggtaagtt attatcctga agtacttcaa caaaaagaat ttattgaaaa agttatccgt
    18001 acagaagaag aacgtttcaa tgaaacaatc aatgaaggtt tggaaatctt aaatcaagtg
    18061 atccaagaag taaaaaacga aaacgggacg actctagcag gaaaagacat cttcaaacta
    18121 tacgacacat atggcttccc ggtagaatta acggaagagg tggcagaaga tgaaggactg
    18181 aaagttgatc atgctggttt tgaaaaagaa atggatgcac aacgcgaacg tgctcgctct
    18241 gcccgcagta ctgaaaaatc aatgggggta caatctgctt tattgacaga tatcaaagta
    18301 gaaagtaaat tcattggata cacagaacaa gaatcagata gtcaattatt ggtcatcgtt
    18361 aaaggtgagt ctcttttgaa tgaagtcgca gaaggtgaag ctcaaatgat ctttgatcaa
    18421 acacctttct atgcagaaat gggtgggcaa gtagcggatc aaggatggat catgaatgaa
    18481 gtaggcgaaa tcgtagcagt tgttacagat gttcaaaaag cgcctaatgg tcaatttatg
    18541 cacaccgtag aagtaaaagc accaatgagt gaaggacaaa cctatcatct attgatcgat
    18601 caatcgatgc gcaatcggat catcaaaaac catacagcaa cccatctact gcacaaggca
    18661 ttgaaagaag tacttggcga gcatgcaaat caagctggtt ctttagtcgc tcctggacat
    18721 ttacgattcg actttaccca ctttggtcaa atcacttcag atgaattagc tgaaatggaa
    18781 agaatcgtca acgaaaaaat ctgggaagct atcccagttg aaacaattga aacagatatc
    18841 gatacagcca aaaacatggg tgcaatggct ttgtttggtg aaaaatacgg acatgacgtt
    18901 cgagtagtaa atattggcgg ctggtcaatc gaactgtgcg gaggcacaca tgtagcaaat
    18961 acagaaaaca tcggaatctt caaaatcgtt tctgaatcag gaattggtgc aggcgtgcga
    19021 cgaatcgagg ctgtaacaag taaagaagcg tacgaactac ttgaagaaga agaaaaacaa
    19081 ctaaaacaag tagcaacaat tgttaaatca cctcaattga aagaagttgt ttcaaaagcg
    19141 gaacaattgc aacagcaagt aagagagcta caaaaggaaa atgaacaatt agctagcaag
    19201 ttagcgaatc aacaagctgg tgatgtattc aaagatccag aaacgatcaa tggaaaaaca
    19261 atcattgcag cacaagtaaa agtgaaagat atgaatcagc tgcgtcaatt agctgaccaa
    19321 tggaaacaaa aaaatatttc agatattctt gttttagcaa acgaacaaga aggaaaagtc
    19381 agtctgctag cggcaatgac gaaagaatca aatgaagctg gactaaaagc tggcgacttg
    19441 attaaagcaa tcgcaccgaa agtaggcggt ggcggtggtg gacgtccaga catggcacaa
    19501 gctggcggga aaaatcctgc tggtattcca gatgcactta aagctgcgaa agaatgggtt
    19561 gcgcaacaat agggcgttta cgtttgacag aaaaacaaaa gttcaaacat tgaacgaaac
    19621 atgattatgg gggcgaagac aaacaagtct tgccctcata cttttttcaa ttggatggcg
    19681 catctaatca aatggtcgga ttcggatggt ctttagttga cgaaagtgac agaaattttt
    19741 acgattagta taaataaaaa ggggtgaaaa aatgaatagt agaaataaga cctttcgact
    19801 agtcttacgt gcgatcttgt tagcaatcat catcgtacaa gcgatggttc cttggctggg
    19861 gtttattcca ttaggattca ttagtttaac aattatccat atcacagtaa ttattgctgc
    19921 agtagtatta ggcccaaaag atggcatggt gattggcttg ttttggggaa tcgctacaat
    19981 tgttcgtgcc tatgccatgc caacaacgcc atttgataca ttagtgttca cgaatccgat
    20041 tatctcggta gttcccagag tacttgttgg cttagtagca ggcttggttt tccactggat
    20101 ttatcaaaaa aataaatcaa taacgatcag ctcagccttt gcaggggtac tgggttcatt
    20161 ggtcaataca gtcttagtgt taggattcat gggattattt tatacaggtg caacggcaga
    20221 tgcctatggt gtagactctg ctcttttatt taaaacctta gcaggggttg cagcaatcaa
    20281 tgggatacca gaagcaatag gtgcgggaat cataacacca ttactagcaa aagcgttatt
    20341 tgcagcaaca ccgttgaaac cagaataatg accatctttt aatagtaata ggacgcacga
    20401 tagatagaaa aaggaagaga ggagaggaaa acaatcgtcc tctccatctt tttacctgag
    20461 caacaagcaa actagaaggg gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac
    20521 aaataccgca acagcggaca aaaaaaggag tactagcggg tatcttagga ttggttacga
    20581 atctgatctt gtttgtctct aagctaatga tcgggctttt ttcaggaagt gtctccatta
    20641 tggctgatgc agtgaacagt ctatcagata ctgcttcatc catattgact ttgatcggat
    20701 ttaaaattgc tgcaaagcca gcagatcagg aacatcctta tggacacgaa cgcttcgaat
    20761 atatcagtgg gttatttgtt tcgatcatca ttacttatgt aggatttcaa tttttagagt
    20821 cttctgctga aaaaatcatc catccagaaa atgtctcttt aacacccatc gtcttttttg
    20881 tcctaatcat ttccatcttt ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc
    20941 aatccattga ttctgaaaca ttgagagcta cagcaacaga tagttataat gatgtcttta
    21001 ctacactggc tgtattgctt tcagcagcaa tcgaatgggt aactggatgg cgtattgatg
    21061 gatatatagg ttttgcttta gcattatata tcctttatag tggattgacg atgcttcgaa
    21121 attttgtata cgaactatta ggaagtcgcc caacgccagc agagattgaa gaaatggaag
    21181 agcatttaaa tgcttgcgat gatatacttg gctatcatga tttattggtg cataactatg
    21241 gacccaataa acgctttgca tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg
    21301 tggctcataa gattattgat ctgatagaaa aagactttaa aaagtctttg gatgtggaac
    21361 ttgtctgtca cttggatcca atgcctgttg ataacgaaaa atactataat atcttgcggc
    21421 agttaaagca aattcttctt gaaacaggta cagggcttaa tattcatgat tttagagtaa
    21481 ttcatcaagg aaaaacgctg caatttgatg tagtggtccc tgagaaattc cagtttgttg
    21541 atacagaact agaaagaatc ttacgtagtc aaatagaaga aaaaatcgga gattaccaat
    21601 tggaaattac gtttgatcac aattatcttt tatattaaat gagaaactat aaaaagactc
    21661 cttaaagttt ttaatttaaa gagtcttttt ttcttttaat tgtttatgga gagcctgacg
    21721 tgcaagatta tctgctcctt tatttttgtt ttcgggcaac cattgcagga tcaataaaga
    21781 aaattcggat agaagttctt ggattctttg aaggtaggga aggaagagct cgttcttcgt
    21841 tgcgtttttg tcgaccgtag aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc
    21901 attctgtaag tcgtttttct ttagaaattc taaagttttt aaaaagactt cgaactctgc
    21961 ttgatggttt gtccctatag aaagcgggat agatagctgt tcttgtttac cttgatagac
    22021 aatcaagatt ccacctccac taggaccagg gttgcctttt gtagaggcat caatgtatac
    22081 ttttaacata aaaagagatt ctcctttttg ttcaaactaa tgttatggta agattatatc
    22141 attactgaat cggtgagagg ttagagaatg aaaaaaaata ttcgatggca gcctgaactg
    22201 gcacatacga tcatctattg gtcattgacc ttgatcattc tgttttttag tctgattctt
    22261 tctttagaaa acacaggtcc ttattggaaa agcaattttg ttttaggtat ttttcttgtt
    22321 tttctatttc taggtttgcg gcgttctttt attttaaaat caaactctgt gattatacgt
    22381 tatgctgctt tttggaaaga ccgagaaata tctgtcggac agattcgatc tttgacgcca
    22441 actaatactg gggtgcaaat gatgttgcaa gatgagaaag ccccatctgt tttcatgatg
    22501 cgaaaaaaag atcaagtagt cctttttgat cattttgaaa aaaacggacc caaatcagaa
    22561 tcaattcctt atccttatac gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa
    22621 aaaacgaatt tttacaggaa aagaaaagag tgagacgtac ggttcatccc gtttagcgtc
    22681 tcactctatt tgtttatcta ttcatgcata ctagtatcgg tagtctgaac gttgcctttc
    22741 ttgacatggg cagcttgtgt tcctcgattc ccttcgatga tctcaaattc gaccacttga
    22801 ttttcttcca aagttttgaa tccctcttct tcaatcgcag taaaatgtac aaaaatttct
    22861 tctgtgtcat cataactgat aaagccgtat ccttttttgt tgtcaaacca ttttacaatt
    22921 cctgtggtca aagcactcac gacctttcta aagtctgatc atagccttca ttataaagag
    22981 tatttataag tacgtcaaat aaattaaaaa cctgtaattt tatgtttttt ttctgaataa
    23041 aaaaaggtat aattggcata tcttaacaaa gaagatattt tgttgaataa agttcgtgaa
    23101 aaggggtaat caaatgaaga atgatctgca aatttcacaa gaaacagagc tagaaccgat
    23161 tgttgaagtg gctagtaaat tagatcttaa ggaagacgat ttggaactgt atgggaaata
    23221 taaggcgaaa atcaattttt ctggaatcaa tcgatcaaag gataacgctg aggggcattt
    23281 gatcttagtt acttccatca atccaacacc agctggcgaa ggaaagtcaa cgatcactgt
    23341 agggttagga gacgcgctca ataaaatcaa taaaaaatct gttattgcat tgcgagaacc
    23401 ctcacttggt cctgtgatgg gaatcaaagg tggtgctgca ggcggaggat atgcacaggt
    23461 attgcctatg gaagatatca acttgcattt tactggtgac atgcatgcta tcacaacagc
    23521 taataatgca ttatctgctc ttttagataa tcatattcat caaggaaatg aattgaacat
    23581 cgatgcccgc cgagtgattt ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa
    23641 agtaattgtt ggcttgggtg ggccaatcca aggtgttcca agagaagatg ggtttgatat
    23701 cacagttgcc agcgaaatca tggctatctt gtgtcttgcg gcagaccttg aagatttaaa
    23761 ggctcgtctt gcaaggatcg ttgtcggcta tacatttgac cgtcagcctg tgactgctgg
    23821 agacttgaaa gcagaaggcg ctctggcatt actgctgaaa gatgcaatca agccgaatct
    23881 tgtacaaaca atttacggaa caccagcttt tgtccacggg ggaccgtttg ccaatatcgc
    23941 tcatggctgc aatagtattt tagctaccaa gacagcattg agattagcag attacgttgt
    24001 cacagaggca ggatttggtg cagatcttgg cggagagaaa tttttagata tcaaagtacc
    24061 taacttgaaa aaagcgccgg atgctgtggt gattgtcgcc actgtccgtg cattgaaaat
    24121 gcatggcgga atgaaaaaag atgaacttaa aaatgagaat cttgatgctt tgaaagtagg
    24181 ttttgcaaat ttaaagcgcc atatacgtaa tatggaacaa tatcagctac ctgtgatcgt
    24241 cgcaatcaat gaattcgtaa cagatacaga caatgaactc acactgttgg agcacttgtg
    24301 tgaaaatcag gggattcttg ccaagagagc gagtgtatgg gcagatggag cagaaggcgg
    24361 cgttgattta gcaaaagctg tagtacgatt gattgataga aaagaagcta attacaagcc
    24421 attataccgc ctagaagaaa cgatacaaga aaagacagaa acaatcgtca agaaaattta
    24481 cggaggaaat ggtgttgttt tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa
    24541 gaatggatgg gacaaactgc cgatttgtat ggcaaaaaca cagtattcat tttctgatga
    24601 tccttcactg ctaggtgcac cggaagattt caccatcacg atccgcgaaa tcatcccaaa
    24661 attaggggca ggatttttag tagccttgac tggagatgtt atgacgatgc ctggtttgcc
    24721 taaaaaacct gctgcattga atatggacgt gacaaatgac ggagaagtgt tgggattgtt
    24781 ttaagcttga ttataatcta ccatcgaagg agctgtattt tgactacagc ttcttttttt
    24841 agacgatagg gaattaattt tgtttacact aggatcattc tttttcttta ttgtattttg
    24901 agctatacta aagcataata ggagggtatg atgaaaacga catttataag aaaaaccggt
    24961 ttttacggta tgggtagctc gattcagcta agaatcaaca atgaaaaacg cagtgttctc
    25021 agtcatgaac aaaagataac gcttgatctg gaagtgccct ttacgatgca ggtcaccttt
    25081 tattggctaa aaagccccgt ttatacagtg aaagaagcaa agcctgctta tgtgatcaca
    25141 atgaattact tgattcttca aatctatccg tttttatttc tagcaactgg gttgggggcc
    25201 gttttcctac aaaatttatt tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt
    25261 ttctatataa aaaaccgggc atatttgatc aaggaggaaa attatggcaa actctgatct
    25321 ttttttaatc agtttcaatc gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg
    25381 aaatatggga tttaccgaac ttgtcagacg cctagctcaa agaaaacaat taatgatcag
    25441 aaaatacgaa gatgatcttt tacagcttgc tcagttaagg aatgcgatcg tccatgatcg
    25501 gatcgcagtc gattttgtca ttgcagagcc aaatgattgg acagtcaagc gaatccagct
    25561 gatcgaacaa gaactgattc agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac
    25621 aggttttgaa caagatcttc cgttacttga actgttaaag atagtagcag aaaaaaggta
    25681 ttcacaattt ccgttataca ataaaggaaa atttgtagca ttgattactt tacgtaatat
    25741 cgggttttgg ctagcaaaag aaagccaaaa aggtccggtt gatttaacaa ataaacgagc
    25801 gttggatttg atcatccaaa acggaaagta cacaaattat cattttgttc cagctaaaac
    25861 acatatttac gaggttgaag cgatgtttcg tgagcaaggg actttggaag ccgtacttat
    25921 cactaaagat gggaatcccg atggtaatct gctcgggatc gtacgacctc gtgatattta
    25981 taaacaaata gaaaaggatt gattttttgt taattattta ttggatattc agcgctttgc
    26041 tagtaggagt ggatcagttg gtcaaatggt ggatcactga taatctagcg cttggagaga
    26101 cgaaaaatct tataccaggt attttgtcac tgaatcatat tcgaaacact ggagccgcat
    26161 ggagtatgct agaagggaag atgtggttct ttactatagt gacactcatt gcagtcgttg
    26221 tgattctcac attgatgatt aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat
    26281 tgagtttgat tctagctggt gcgattggca attttatcga ccgtattcgt cttggctatg
    26341 tagtagatat gttccagaca gattttatga gctttccgat ttttaacgta gcagatgtga
    26401 cacttgtatg tggagtgatt tgtatgttaa tttatattat tttagatgaa aaggatcaaa
    26461 gaaaaaaatg acagaactta aaacgacgat tcaccatgaa tctggtagga tcgataaagt
    26521 attgactaat ttattcaccg attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg
    26581 agcggtttct gtcaatggac agccagtcaa agcaaattac aaagtaaaac aaggagacga
    26641 gattactgtc ttgatccccg aaccagaaac attagatgtg gtcccagaaa atatcccgtt
    26701 gacgatcgtt tacgaagatg aagatgtagc ggtgataaac aaacctcaag gaatggtcgt
    26761 tcatccttca gcaggtcata cgcatggcac aatggtcaat gcgctgatgt accatatcaa
    26821 ggatctttct acgatcaacg gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga
    26881 tacttccgga ctgctaatgg ttgccaaaaa tgaccaggca cacgaatcat tagctcagca
    26941 attaaaagat aagacctctt tgcgcaaata tgttgcttta gtccatggag agattcctca
    27001 cgaaaaagga accatcaatg cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc
    27061 gataagagaa gatgggaagt cggctgtcac acattttact gtattggaac gtttcgaagg
    27121 atttactctg gttgaattaa aactagaaac cggaagaaca caccagatcc gtgtgcatat
    27181 gaagtatatc ggttatccgt tagctggaga tccagtatac ggaccgaaga aaactttgaa
    27241 aggcaatggt cagtttctcc atgcgcaaat gttaggtttt acgcacccga ctacaaacga
    27301 aaagatgatc tttgaagcac ctcttccaga agtttttgaa aaaactttag aaaaattaag
    27361 aaaaaacatt gcattttaat ttagataacg gtatagtaat taacaacgaa agcataaaac
    27421 ttaatagaat tcctttaagt ttagtcccgt gaggctaaga agggtgggag caaggatacg
    27481 tatggtgcgc cataagtaga ttgctcattg acatagtata gctatcagtg tatcagtata
    27541 cgctcagtct aaacattcct cctgtcctct tagggcagga gtttttttat gcaaaaaagt
    27601 agggggaatg aaaatgcaag caaaagaagt agtagatcag gtaaccatga agcgagcatt
    27661 gactcgaatt acttatgaga ttatcgagag aaaccatagt atccaagata tcgtattggt
    27721 aggtatcaaa acacgaggaa tctacattgc atcaagaatt gccgaacgcc tgaagcagct
    27781 agaagatatc gatattcctg taggagaact ggatatcact ttataccgag atgacaaaaa
    27841 agaaaatcca gaagaaccgg aacttcattc ttctgacatc ccagtttcct tagaaggaaa
    27901 agaagttatt ttgattgatg atgttttgta tacaggacga acgattcgag cagcaatgga
    27961 cgcagtaatg gattttggca gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg
    28021 gcatcgagaa ttgccaatcc gtgcagatta tgttgggaag aacattccaa catctcgggc
    28081 ggaagagatt ttagtagaga tgcaagagct tgatggacaa gatcgcatca tgattttgaa
    28141 agaggaagat tagaggagag agaaacatgt cggagaaaaa aggatttcat aatgatgacg
    28201 tggtgttaga tatccacgat cgcccaaccc caggacattg ggtaggatta agtttacaac
    28261 acttgttcac gatgttcgga gcaaccgtgt tagtccccat cttagtaggt atcgatccag
    28321 gtattgcgct agtcagttca ggactaggta cattggtgta cttatttact actaaaggaa
    28381 agatacctgc gtacttagga agcagcttcg ctttcattgc agcgatgcag cttttgatga
    28441 aaacagatgg ttatccagcg attgcacaag gagcagtcac aactggatta gtctatttga
    28501 ttgttgcgct gatcatcaaa aagatcggtt cagcatggct ggataaaatc ttgcctccaa
    28561 tcgtcgtcgg tccagtcatc atggttatcg ggttgggact tgcggcaaat gcagcaacaa
    28621 atgctatgta taatcagggc acgtatgatt tcaagtatat tgctgtagcg ctgatcacat
    28681 taggattaac aattttcttc aacatgtggc taagaggttt tctcggattg attccgattt
    28741 tactaggtat tattagcgga tacatcgtag ccttgctgtt tggaatcgtg gatactaagc
    28801 caatcgtaga cgctgcctgg tttgctgttc ctaactttga gattcccttt gtacaatatg
    28861 aaccaaagct atatcttgga gcaatcacaa caatggcacc catcgctttt gtgacgatga
    28921 cagagcatat cggccatctt atggtattga ataaactaac taaacgtaat ttctttgaag
    28981 aaccgggtct tcataagacg ttgacaggtg atggattagc acaaatcgtg gcagcctttg
    29041 tcggaggtcc tcctgtaaca agttacggtg aaaacattgg tgttctggct atcacacgtg
    29101 tccatagcgt gtttgtgatc ggtggagctg cagtatttgc agtcatttta ggattcgtag
    29161 gaaaattaag cgctttgatt ttaagtatcc ctggaccagt catctcagga atcagtttta
    29221 tcttatttgg agtaattgcc gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt
    29281 ttgataaaaa gaaaaatctg ttgattgctt cagttatctt agtagtagga attggcggac
    29341 tcgtctttga agtaggcaca tttacattgt cggcaatggc attagcaact gtacttggca
    29401 tcattttaaa tcttatctta ccagaaactt cacgaagcga agaaaactaa ggaggaaaca
    29461 ttatgatcat caaatcagaa cgtatcagct taaagcattt attgaccgtt gaggcactta
    29521 ctgatcaaga agttatgggc ttgatccgca gagggcagga attcaagcaa ggtgctaatt
    29581 ggactccgca aaagcgccag tactttgcta caaacctctt tttcgaaaat agtactcgca
    29641 cacataaaag cttcgatgta gcggagaaaa aactagggct agaagtgatt gaatttgaag
    29701 caagcacaag ctctgtccaa aaaggggaaa cactatacga tacagtactg acgatgtcag
    29761 cattaggcgt agacgtagcg gttatccgtc atggtgatga gaactattat gacgaactga
    29821 tacaaagtaa aacaatccag tgttcgatta tcaatggagg agacggaagc ggacagcatc
    29881 caacccaatg cttgcttgat ttgatgacca tttatgaaga atttggtcat tttgaaggtc
    29941 tgaaagttgc catcgtaggt gatatcactc actctcgcgt agctaaatcc aatatgcaga
    30001 tgttaaaacg tctaggcgca caagtattct tctcaggtcc tagagaatgg tacgacgatg
    30061 aattcgaagt gtacggacat tatatgccat tggatgaact actagatcaa gttgatgtca
    30121 tgatgatgct tcgggtgcaa cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag
    30181 gctaccatca ggaacatggg ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa
    30241 tcattatgca cccagcacca gttaaccgtg atgtagaatt agctgattca ttagtggaag
    30301 gattacagtc gcggatcgtt cagcaaatga gcaatggagt attcgtcaga atggcaatct
    30361 tagaagcagt gctaaatgga aaagcgtaat tataggagga tgaaaaaatg aaaacactca
    30421 ttaaaaacgg tcagatcaat acacgaaaaa atatgacgac accagcagaa atttggatag
    30481 aagatgggcg aatcaaagca atcggtacag gattctcaga agccgaattt gatgaagtgt
    30541 ttgatgcaaa gggacaattg atcacacctg gtttagtgga cgtccatgtt catttgagag
    30601 agccaggatt tacatacaag gagacgatcg aagctgggac tcgctcagct gctcgtggcg
    30661 gtttcactac tgtatgcgcg atgccgaatt taaatcctgt acctgatacg gcagaaaagc
    30721 tgagacaagt atatgacatc attagaaaag atgcagttgt gaaggtattg cagtatgcgc
    30781 caatcacaga aaatctgcga agtgagaaat tagttgatca agaagcactg attgaagaag
    30841 gtgcttttgc tttcacaaat gacggtgtag gtgtccaaac agctgggacg atgtatctgg
    30901 ctatgaaaga agctgcaaaa aacaataaag ccctagtagc ccatacagaa gatgaatctt
    30961 tattatttgg cggcgttatg cacgctggca agaaggcaga agaacttggt cttcccggga
    31021 tcttaagcgt aaccgagtct tctcaaattg ctagagatct gttgctagct gaagcaactg
    31081 gtgttcatta ccatgtttgc catgtttcta ctaaagaaag tgtccgtgta atcagagacg
    31141 caaaaaaagc aggaatccac gtaacagcag aagtttcacc ccatcatctt attttgatcg
    31201 acgaagatat tcccgaagat ttcggctttt ggaaaatgaa cccgccgcta agaggacggg
    31261 aggacagaga agctttgatc gaaggcttgc ttgacggtac gatcgattgc atcgctacag
    31321 accatgcccc ccatggatta gaagaaaaaa gccaaagttt catgaaatca ccatttggta
    31381 tcgtaggaag cgaaacagcg tttcaactga tctatactca ttttgtagaa actggtcgat
    31441 tcacgttgga acaagtgatc aattggttgg cagttaaacc ggctgagatt tttggtttga
    31501 atgcaggaac gctaacggtt ggtgcccccg ctgatgtagc agtttttgat attacacaaa
    31561 cttgcacgat cgataaagaa gactttttat caaaagggga gaatacacct tttatcggct
    31621 ggaaagtaaa aggcgaaaca caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg
    31681 gagaataaaa gtatggaacg tttgcttata ttagaagatg gaacagtttt cgaaggaaag
    31741 gcttttggtg cagatgctag tgtcttcgga gaaatcgttt ttacaacaag tatgactggt
    31801 tatcaagaaa ctatcactga tcaaagtttt aacggtcaaa tcattacatt tacctatcct
    31861 atggtgggta attatggggt aaatagagat gattatgaat cgatttctcc aacatgcaaa
    31921 ggagtagtag taaaagaaca tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta
    31981 gattcatttt tgaaacaaaa aggaattcct ggaatatcag gtatcgatac gcgcgccctt
    32041 actcgcaaaa tccgtgaagt cggaacgatg aaagctagta tagttgatgc tggagattcc
    32101 ttcgaacatg catttgatca gctgaaagca accgttctag caaccaatca agtgcagcaa
    32161 gtctcaacta acaaacctta tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac
    32221 ttcggcttga aacatagtat tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta
    32281 cttccctata acacagatgc cgagacgatt ttagcgctat caccagacgg agtcatgctg
    32341 acgaatggac ctggtgatcc aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc
    32401 caaggaaaag taccgatttt tggtatttgt ctcggtcacc agttatttgc tttagccaac
    32461 ggtgcagata cctataaaat gaaattcgga catcgaggat taaatcaccc ggtacgagaa
    32521 gtagcgaccg gaagaattga ttttacttct caaaatcatg gctatgcagt cgatgaagcc
    32581 acgattgatc cagaaaaact catcgtaact catgtagaag taaatgacgg aacagtagaa
    32641 ggagtcaaac acagagatta tcccgctttc agtgtgcagt ttcatccaga tgctgcccct
    32701 ggtccgcatg atgcactaca tttatttgat gaatttatgg aaatgatgga cgcaggaaag
    32761 gagcagtaaa cagatgccga aacgtacaga tatcaagaaa attatggtga ttggatctgg
    32821 tccgatcatc attggtcaag ccgcagaatt tgactacgca ggaacacaag cctgccttgc
    32881 cttaaaagaa gaaggatatg aagtggtttt agtaaattct aatcctgcga cgatcatgac
    32941 ggataaagaa gtggcagatc aagtatatat cgaaccgatc acacttgaat ttgtatcaag
    33001 gattcttcga aaagagcgtc cagatgctct ccttccaact ttaggcggac agacaggcct
    33061 caatatggca atggagctgg cagcatcagg tattttagaa gaattgaaca tagaacttct
    33121 aggaacaaag ctttctgcga tcgatcaagc agaagatcgt gatctattca aacagttgat
    33181 gaaagaattg aatcagccaa tccccgagtc tgaaatcgtc aatacagtag aagaagctgt
    33241 agattttgca aataaaatcg gctatcctgt catcgttcgt ccagccttta ctttaggcgg
    33301 aactggtgga ggtatgtgtg aaaatgaaga agaattacgt atcgtatcag aaaatggatt
    33361 aagtctttcc cccgctaccc aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat
    33421 tgaatatgaa gtgatgagag attcggctga taatgcaatc gttgtctgca atatggaaaa
    33481 ttttgacccg gtgggtatcc atacaggtga ttctatcgta tttgccccta gtcaaacatt
    33541 gtctgaccat gaatatcaaa tgcttcgtga tgcttctctt tcgattattc gagcacttaa
    33601 gatcgaggga ggctgtaatg tccaactcgc tcttgatcca catagtttta attattatgt
    33661 gattgaagtc aatccgcgtg tttctcgttc atctgcgtta gcaagtaagg cgactggcta
    33721 tccgatcgct aagctagctg ctaaaatagc gcttggatta acattagacg aaatgaaaaa
    33781 tcctgttaca ggaacgactt ttgcagaatt tgaacctgca ttagattatg ttgtagctaa
    33841 aattcctcgc tggccgtttg ataagtttga aaacggagaa cggcttttag gcacgcagat
    33901 gaaagcaact ggtgaagtga tggctatcgg tcggaatatc gaagagtctt tattgaaagc
    33961 tgtgcgttct ttagaaatcg gtacacacca tatcgaactt cctgagttgg cagacatcac
    34021 ggacgatgac ctaatccaaa aagtggttcg agcacaagat gatcgcctgt tttatgtagc
    34081 tgaagctatt agaagagggt acaccataga agaattagcg gatttgacaa aaatcgattt
    34141 attcttttta gataaactgt tgcatatcgt agaaatcgaa cagcagttga aacagcagcc
    34201 gttggatgac aagatattga aaacagcgaa acaaaacggt tttacagaca aaaaagtggc
    34261 ggagctgtgg gaaataaacg aagaagccgt tcgccagatg agaaaagaaa atgatttgat
    34321 tcctgtatat aagatggttg acacttgtgc agcggaattc gaatcaaaaa caccttattt
    34381 ttatagttcc tatgaatatg aaaatgaaag ttcacgatca gaaaagccct caattctagt
    34441 actgggatct ggtcctatcc gtatcggaca aggggttgaa tttgactatg cgactgtgca
    34501 ttctgtcaaa gcaatccagc aggcaggata cgaagcaatc atcatgaaca gcaatcctga
    34561 aacagtttcg actgactttt ctatttcgga taaattatat tttgagccat tgaccttcga
    34621 agatgtgatg aatgtgatcg agctagaaca gccgatagga gtaatcgttc aatttggtgg
    34681 tcagacagca atcaacttag ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac
    34741 aacgatcgaa gacttggatc gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga
    34801 gttggatatt cctcaacctt taggggatac tgctacaagt aaagaagaag ccgtggttat
    34861 cgcttcgaga attggttatc cagttcttgt tcgtccaagc tatgtattag gcggacgtgc
    34921 gatggaaatc gttgaaaacc agcgagattt agaagattac atggaacatg ctgtgaaagc
    34981 atctccagaa catccagtac tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga
    35041 tgcgatttgt gacggtgaaa cagtactcat cccagggatc atggagcata ttgagcgtgc
    35101 tggtgtccat tcaggggatt caatggctgt gtatccacct caaacattat ctgaatcgat
    35161 caagaaaacg atcgaagatt acacgatccg cttagcatta ggtttgaatt gtgtcgggat
    35221 gatgaatatc cagtttgtca tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg
    35281 agcaagtcgg actgttccat tcttaagtaa ggtgacagga attccaatgg cacaaattgc
    35341 gacgaaagct attttgggag agaaactaaa gacacttggt tatgaaaacg gactttatcc
    35401 agaaacaaaa gaagttcatg ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt
    35461 tgatacttac ttagggcctg aaatgaaatc gactggagaa gtaatgggat ctgacagaca
    35521 attagacaaa gctttgtaca aggcattcga agcttctgga ttacacttgc ctgaatatgg
    35581 agccgtatta tttacgattg cggacgaaac aaaagaagaa gcactagaat tagctaaacg
    35641 ttttacagaa attggctata gtctgatagg aacgaaacat acagctgctt atttcgaaaa
    35701 aaatggtctg gtcgttgaaa cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt
    35761 agtcgactta ataagagatg gtaaaacaca agtggttgtc aatacgattg ataaagatcg
    35821 gggaaatgct tctaaagacg gtttcatcat ccgaagagaa gcagttgaac atggtattcc
    35881 attgtttact tctttagaca cagcagatgc gatcgttcga gtgatggaat cccgtgcttt
    35941 ttcaactaac cctatttaac ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc
    36001 aggaggggac agatgaaaca agaaatgatg gaaatcgttc agcaaaaaca gctggcacca
    36061 aagatttttg aaatgacatt gaaaggtgat ctagtaaaag aaatgcagca accaggacag
    36121 ttcttgcata ttctcgtgcc gcgtgcagac ttgctattac gccgaccgat tagcttgaat
    36181 catatcgacc acgaaacaaa tacttgccgt atcatttatc gggtggaagg tgaggggact
    36241 agagtcttct ctactctttc tgccggagac catttagatg tcatgggtcc tttaggcaat
    36301 ggttttgatt tgtccaatct tcatacagga gacgaagtat ttataatcgg tggaggaatc
    36361 ggtgtccctc ctctatatga gttatcaaaa cagttagtcg aaaaaggaat taagccaacg
    36421 cattttttag gttttgccac acatgaagtc gtttattatg aaaaagaatt taatcgtcta
    36481 ggagaaacaa gaatcgctac tgacgatgga acatacggta tgcacggaaa tgtcggtaat
    36541 ttattgttag gaacgaagac ggaacctaca gctgtttttg catgtggttc aaacgggtta
    36601 ttgaagacag tggaacagct gttttcttcg catcaaaatg tccagttatc attagaatcc
    36661 cgcatggcat gtgggatagg ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa
    36721 acaggaacaa aaagtgtgaa agtttgtgac gaaggaccga tttttaaaat aggagaggta
    36781 attatatgaa tgagaatcct ttagcggtca aacttcctgg cctagaatta aaaaatccta
    36841 tcatgccggc aagcggctgt ttcggttttg gcaaagaata cggaaaatac tatgatttga
    36901 atcagcttgg atcgatcatg gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca
    36961 cgccaagagt ggcggaaaca ccatctggca tgttgaatgc aattggtttg caaaatcctg
    37021 gtctagacgt tgttatgcat aaacttcttc cagaattaga ggagtacaat gagttaccca
    37081 tcatcgcaaa tgttgctggt gcttgtgaag aagactatgt agaagtttgt agcaaaattg
    37141 gcgaagcacc aaatgtgaaa gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg
    37201 gcggaattgc ttttggaaca gattctgatg ttgcttttca attaacacaa gcagtaaaga
    37261 aagtttcttc tgttcctgtc tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa
    37321 tcgctcaggc aatcgaagct ggaggggcag atgggtttac gatgatcaat actcttcttg
    37381 gtatgcggat tgaccttaag acaagaaaac cgattttagc caatcaaaca ggtggactct
    37441 ccggaccagc aatcaagcct gtcgctattc gtttgattca ccaagtagca agtatatcca
    37501 gccttccaat cattgggatg ggcggcgtgc agacagtgga tgatgttctt gagatgttta
    37561 tggctggagc aagtgctgta gctataggga ccgcgaattt caccgatccg tatatctgtc
    37621 ctaaactaat taaagagctc cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa
    37681 gattaatcaa agaagtgaga gaggaacgtg aacgatgaat caactagacc aacgaccaat
    37741 tattgcatta gatttttcaa ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa
    37801 agaagaaaaa ttgtttgtga aaatcggaat ggaattgttc taccaagaag gacctgaaat
    37861 cgtacgttat ctaaaagagg ctggtcataa tgtattttta gatctgaaac ttcacgatat
    37921 tccgaatacg gtcgaaaaag caatgcgggg attggcaaag cttggtgttg atctcacctg
    37981 tgttcatgcc gctggcggta ttcgtatgat ggaggcggca atgcgaggat tagaagaggg
    38041 gactccagaa ggtggaaaac gtccgctact tctagctatc actcagctta cttctacaag
    38101 tgaagaagaa atgcatgccg atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca
    38161 ttatgctagc tgtgccaaaa aagcaggatt agatggtgtg gtttcttctg cttgggaagt
    38221 agaagcgatc aaagaaatgg caggagacga atttgtttgc ttaactccgg gtataagacc
    38281 agaaggaaca gtcgcaggag atcagacaag agtagtcaca ccttctcagg caaaaaagat
    38341 cggctcaact tttatcgtta taggtcgacc aatcactcag tcaactaatc cttataaagc
    38401 atatcaaacg attcaaacag aatggagcta acctaaaatg aatgttgaac aaagtatcgc
    38461 aaaagactta ttagaaattg aagcagtatt tttaaatcca agtaatcctt ttacatgggc
    38521 aagtggaatc aaaagcccaa tttactgtga taaccgaatc acgatgagct atccaaaagt
    38581 ccgaaaagag atcgcaaaag gattagcaag caaaatcaaa gaagcttacc ctgaagtaca
    38641 agtgattgct ggaactgcaa cagcaggaat ccctcatgcc gcatgggtag cggaaatact
    38701 ggatttgcca atggtctata ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat
    38761 cgaaggacgt atatttgaag gtcagaagat ggttgtgatc gaggatttga tttctacagg
    38821 cggaagtgtc ttggaagcag ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt
    38881 tgcggctatc tttacgtatg agttgccaaa aggaaaagca aatttcgaaa aagccgaaat
    38941 ccctcttatg acgttgacca attattctgt attgattgaa gcagctttag aagatcgtta
    39001 tatcgatgag caagaattaa ctctgctaaa agaatggaaa aaagatcctg aaaattggca
    39061 gattgaagcg cgcttgatga agatagagaa atccgatttg tgtctccaga atcatcaatt
    39121 ggaatagcaa aaatccaaaa actgattgcg agaagcatct tttcatcagg tatgaaaaga
    39181 aacttctatc gtgatcagtt tttgattttt ttaaaattca tttttctaag actggttagc
    39241 agaaagaaga gaataatctc ttctcttttt tctattatat atagattttc tctatttttt
    39301 ggtaagggag ctgtttcaaa aatggtaaag agcggaaatg ttgcgaatat cagttattta
    39361 atgatatgat attcggaaaa agaaatctca aagggaggta acttttatgg ggttgaatga
    39421 atttatttta ggattgaaca atttagagac gatcacaagg gcacctggat tttttaaata
    39481 tacagagcat acagttgcag cacattcgta tcgtgtagca tctattgcgc aagttctagg
    39541 agacattgaa gaagcgaatg gacaaccgat cgattggaaa ctgctctatg aaaaatcatt
    39601 gaatcatgac tacacagagc gttttattgg tgatatcaaa acgccggtga aatacgcaag
    39661 caaagagtta agagggatgc tgcaaacagt tgaagaaaag atgacggacg aattcatcaa
    39721 ccaagagatt ccagctgagt tccaagaagt ttatcgccgt cggttgttcg aaggaaaaga
    39781 tgaaaccgta gaaggagaac tattagccat ttcagataag gttgaccttc tttatgaatc
    39841 ttttgatgaa atcatcaaaa ataacccgga aaaagtttat aaggagatgt tcattgaggc
    39901 agtgactacg attaaaagct ttgaacatcg gccttctgtt cagtatttct ttaaagagat
    39961 atttccggaa ctattgaacc aggaatttta tggaaaagag gcgtttttag aaaccgtctc
    40021 aacgattgta aagaaagaag aaaaataaaa atgatatgag atgcaggggg aagtatagtt
    40081 gaaaaagaat atggatgtcg aatggggcta ttctggagac aaaggtccgg aaaattgggc
    40141 gagtttatgt gattggtttg cgagaggtgc ggaatttcca ttgcagtcgc ctattcattt
    40201 gaccaagggg gaagaaacca aaatagaagg agacacgctt tcctttcatt atcaaaagca
    40261 acggtttact gacaaggaat tcaaaaacac gattcattta gtaccttttg accaactcag
    40321 ttatgtagag ttcaagaaag aacgctatta tttgacagat attcatttcc acttgccttc
    40381 tgaacatatc atcaacggaa aacaggaaga tattgaattt cattttgttc atatgaacag
    40441 caaaaaagag aatctagttg tgggtgtcat gtaccagcta atggtacagg aaggttggct
    40501 gtataatcaa gaaaatgggg aaagttggaa cttagaaaaa catgagcatt gggtaaatcc
    40561 ggatgtgttc tttccggagc aaaaaagcca tttccattat attggctcat tgacaactcc
    40621 gcctaccagc gggccaatcc aatggtttgt aatggatcat gtaggaaaac tgaaccaaga
    40681 gtttcttctc cccttcgatg gtcagtatgc tcgaccgaac aaccgaccaa tccagccctt
    40741 gaatggaaga gaaatttatt atttcgagga gtttgagcaa tgaatattca gcagatgaaa
    40801 tacgtggtcg ctgtagcaaa caatggtagt ttcagagaag cggcaaagaa aatgtttgtc
    40861 tcccagccaa gtctttcagc tggtatcaaa gaattagaaa ccgagttagg gatcacgctg
    40921 tttactcgaa cgaatcgagg ggcatattta acagaagaag ggcaagactt cttaaatcgt
    40981 gccgagaaaa tattggtcca gcttgataca ttagaaactc actatctaac gagtgaaaag
    41041 agagacaatt tttcgatcgc tgcccagcat tatgatttct taggtccttt gaccgcacag
    41101 ctactcgagg aattcaaaga cgagatcaaa caatttcgga tcatggagac gacaacagcg
    41161 agagtgatcg aagaagtcaa agagcagcac agtgagatcg gcattattta tatgaatgag
    41221 cataatcaag ctggtatcag aagatattta caacaaggag agctaagtgg ccgcagcatt
    41281 ggaaaattca aaacgcatat ttttttaagg caaggacatc cattagctga acacgcagag
    41341 atcaccaaag aagaattgtt atcttatcct caagttcgtt ttacacagga tggaaataat
    41401 ttcccgtatt tttatgaaga ccttatcgag atccctgatc aagaaacagt gatttatacg
    41461 agtgatcgag gtactttgat gaatatcgtt cttgaaactg atgcttacgc ttcaggatct
    41521 ggtattgtga ttggagggat tcgcgagcac ttgcggttaa tccctttggc agaaggcgaa
    41581 ctaaatgaat tttatatcat ttattcggcg aaacgtccgc tcagtgaaat cgctcaaaga
    41641 tttatcaaag agcttactcg gttactggac cagcagagct aagaaaagca gatgagaacg
    41701 ctgtttattg aacaccgttg atcaaaaaag aggggttgtg acagaatttt gtcacaactc
    41761 ctctttttgt agcaaacaat ttataactaa ttgctgttgt gggaaagtac gcaaatatga
    41821 ttctagaaac actcattgat tacctgtttt gtttcatagc tgtaattttt tcttcttctg
    41881 gtgtcacgat aatcgtcttt tggttttcat aaataacata accaggcttg gcgccatttg
    41941 gttttcggat atgctttacc tgtacaagat caaccggcac ttgggcagag tatcggtatt
    42001 ttgaaaaata ggcagctaac tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac
    42061 ttttaatgat gacgtgcgaa ccgggtatat tttttgcatg aagccaatag tcggtttttt
    42121 tagctgtctt cattgtcagc tggtcatttt gaagattgtt tttaccaaca agaatgagcg
    42181 ttccgtcaga agaaaagtat tgatcaggct gagatggttt cttacgtttt tgttttttta
    42241 agttcttctt tttcaggtat ccttctgaag tcaattcttc tttaattgct tcgatatcca
    42301 ttgggccggc aatttccagt tgagataaga cagattcaag atattggatt tcatctttgg
    42361 cttcttggat ttgttcgcca atcagcttaa cagcgttctt cagcttttgg tatcgatgga
    42421 aatatttctg tgcattttga tttggtgtca atgccggatc tagtgctatt ttgattggcc
    42481 gatcttcttc ataataatta ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca
    42541 gaaaagtagt aagtagttcg ccatttctac ggtaattctc agcgttttcg gattctttga
    42601 gtgtttgttc acgttttttc aatttatttt tatttcgttt tagttcattt tcgatttttc
    42661 gaatcagttc tccgccttgt tgttttgccc gatctttctc tgctttttcg tgataatagc
    42721 tatctaataa ttgactgagt gttggataag ctgttacagc agatgcctgt tcggacaata
    42781 cctgataagc aatcggtgtg aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag
    42841 gctgatggct gatggctgag aaaaattcgt cccatacaac catcttttca ttaggacgtt
    42901 ccagtaatct tttggtcagc tcttgagcga tatcgaaacc gattccctga aactgacgtt
    42961 ggattgcttt tggatctagt tcagtttggg aaagccgctg aaagattttt tctttctcgg
    43021 agctaaatgg gttcaagacc tcttgttttg gcggagcaac atattctaca ccaggaagca
    43081 aagaacgata agtgttttga gagcttccaa tgtgtttgat cgcatccagt atttttccag
    43141 tttctcgatt aactaagacg atcgtactat gacgtcccat taattcaacg atcaatatga
    43201 tattttgtag atcacccaac tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat
    43261 tttcgatttg ttcgatgtct tctaaaatag cgccatccaa atattttcga agcatcatca
    43321 cgaagttagg cggattatca ggattctgat aatcgatttg ggtaatctgt acacgagcat
    43381 aacttgggtg cgcagataat aataatcgat ggtttttgcc gcgtgaacga atcaccagca
    43441 cgatttcatt ttcataaggc tgatggattt tcgagatccg gccagatagt aacgtttcac
    43501 gcaattcgtt gaccatcgcg tgggtaaata caccatcaaa ggacatggtt cattcctcat
    43561 ttcttttcat aagtatattc attataacga aaaaaagaag aaaaaccaat ttggccgaat
    43621 agtaaagcaa aagtctagaa aaatcttgga ctaattacat aaaaaacagt gtttctatcg
    43681 ttgaaatcca aatttttagg t
//
LOCUS       DACDSR010000016        41099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDSR010000016 DACDSR010000000
VERSION     DACDSR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.016"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWK15_000878"
     CDS             complement(38..1189)
                     /locus_tag="IWK15_000878"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9546499.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWK15_000879"
     CDS             complement(1202..1639)
                     /locus_tag="IWK15_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9546500.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWK15_000880"
     CDS             complement(1654..3420)
                     /locus_tag="IWK15_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9546501.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWK15_000881"
     CDS             3684..4361
                     /locus_tag="IWK15_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546502.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IWK15_000882"
     CDS             4364..5491
                     /locus_tag="IWK15_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546503.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IWK15_000883"
     CDS             complement(5691..6131)
                     /locus_tag="IWK15_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9546504.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IWK15_000884"
     CDS             complement(6142..8175)
                     /locus_tag="IWK15_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9546505.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IWK15_000885"
     CDS             complement(8177..9880)
                     /locus_tag="IWK15_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9546506.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWK15_000886"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWK15_000886"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9546507.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IWK15_000887"
     CDS             complement(10963..11988)
                     /locus_tag="IWK15_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9546508.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IWK15_000888"
     CDS             complement(12197..13066)
                     /locus_tag="IWK15_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9546509.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IWK15_000889"
     CDS             complement(13525..15417)
                     /locus_tag="IWK15_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546510.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IWK15_000890"
     CDS             complement(15414..17141)
                     /locus_tag="IWK15_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546511.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IWK15_000891"
     CDS             complement(17157..17807)
                     /locus_tag="IWK15_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9546512.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IWK15_000892"
     CDS             18011..18307
                     /locus_tag="IWK15_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9546513.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IWK15_000893"
     CDS             18479..19099
                     /locus_tag="IWK15_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9546514.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IWK15_000894"
     CDS             complement(19191..20357)
                     /locus_tag="IWK15_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9546515.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IWK15_000895"
     CDS             complement(20369..20722)
                     /locus_tag="IWK15_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9546516.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IWK15_000896"
     CDS             20850..21872
                     /locus_tag="IWK15_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9546517.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IWK15_000897"
     CDS             complement(22311..22745)
                     /locus_tag="IWK15_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546518.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IWK15_000898"
     CDS             complement(22855..24714)
                     /locus_tag="IWK15_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9546519.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IWK15_000899"
     CDS             complement(24832..25992)
                     /locus_tag="IWK15_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9546520.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWK15_000900"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWK15_000900"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9546521.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IWK15_000901"
     CDS             complement(27739..28437)
                     /locus_tag="IWK15_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9546522.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IWK15_000902"
     CDS             complement(28394..30055)
                     /locus_tag="IWK15_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9546523.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IWK15_000903"
     CDS             30273..31673
                     /locus_tag="IWK15_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9546524.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IWK15_000904"
     CDS             complement(31925..33004)
                     /locus_tag="IWK15_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9546525.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IWK15_000905"
     CDS             complement(33090..34049)
                     /locus_tag="IWK15_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9546526.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IWK15_000906"
     CDS             complement(34061..35584)
                     /locus_tag="IWK15_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9546527.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IWK15_000907"
     CDS             complement(35594..36157)
                     /locus_tag="IWK15_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9546528.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IWK15_000908"
     CDS             complement(36163..37026)
                     /locus_tag="IWK15_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9546529.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IWK15_000909"
     CDS             complement(37040..37936)
                     /locus_tag="IWK15_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546530.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IWK15_000910"
     CDS             complement(38111..39382)
                     /locus_tag="IWK15_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9546531.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IWK15_000911"
     CDS             complement(39372..40889)
                     /locus_tag="IWK15_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9546532.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11871 a         8597 c         7104 g        13527 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaa
//
LOCUS       DACDSR010000017        35310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDSR010000017 DACDSR010000000
VERSION     DACDSR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.017"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(174..800)
                     /locus_tag="IWK15_000912"
     CDS             complement(174..800)
                     /locus_tag="IWK15_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546533.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1095..1430)
                     /locus_tag="IWK15_000913"
     CDS             complement(1095..1430)
                     /locus_tag="IWK15_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9546534.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1417..1701)
                     /locus_tag="IWK15_000914"
     CDS             complement(1417..1701)
                     /locus_tag="IWK15_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9546535.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1757..3280)
                     /locus_tag="IWK15_000915"
     CDS             complement(1757..3280)
                     /locus_tag="IWK15_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9546536.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3273..4448)
                     /locus_tag="IWK15_000916"
     CDS             complement(3273..4448)
                     /locus_tag="IWK15_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9546537.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4452..4637)
                     /locus_tag="IWK15_000917"
     CDS             complement(4452..4637)
                     /locus_tag="IWK15_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546538.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4603..6297)
                     /locus_tag="IWK15_000918"
     CDS             complement(4603..6297)
                     /locus_tag="IWK15_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9546539.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6294..6767)
                     /locus_tag="IWK15_000919"
     CDS             complement(6294..6767)
                     /locus_tag="IWK15_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9546540.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6836..6991)
                     /locus_tag="IWK15_000920"
     CDS             complement(6836..6991)
                     /locus_tag="IWK15_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9546541.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7125..7505)
                     /locus_tag="IWK15_000921"
     CDS             complement(7125..7505)
                     /locus_tag="IWK15_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9546542.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7509..7724)
                     /locus_tag="IWK15_000922"
     CDS             complement(7509..7724)
                     /locus_tag="IWK15_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546543.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7727..8134)
                     /locus_tag="IWK15_000923"
     CDS             complement(7727..8134)
                     /locus_tag="IWK15_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9546544.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8400..9875)
                     /locus_tag="IWK15_000924"
     CDS             complement(8400..9875)
                     /locus_tag="IWK15_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9546545.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9865..10713)
                     /locus_tag="IWK15_000925"
     CDS             complement(9865..10713)
                     /locus_tag="IWK15_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9546546.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10750..11112)
                     /locus_tag="IWK15_000926"
     CDS             complement(10750..11112)
                     /locus_tag="IWK15_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546547.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11412..12036)
                     /locus_tag="IWK15_000927"
                     /pseudo
     CDS             complement(11412..12036)
                     /locus_tag="IWK15_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12033..12344)
                     /locus_tag="IWK15_000928"
     CDS             complement(12033..12344)
                     /locus_tag="IWK15_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546548.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12388..12681)
                     /locus_tag="IWK15_000929"
     CDS             complement(12388..12681)
                     /locus_tag="IWK15_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546549.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12875..13522
                     /locus_tag="IWK15_000930"
     CDS             12875..13522
                     /locus_tag="IWK15_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9546550.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13583..14728
                     /locus_tag="IWK15_000931"
     CDS             13583..14728
                     /locus_tag="IWK15_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9546551.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14809..15114)
                     /locus_tag="IWK15_000932"
     CDS             complement(14809..15114)
                     /locus_tag="IWK15_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9546552.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15139..15570)
                     /gene="ruvX"
                     /locus_tag="IWK15_000933"
     CDS             complement(15139..15570)
                     /gene="ruvX"
                     /locus_tag="IWK15_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9546553.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15567..15833)
                     /locus_tag="IWK15_000934"
     CDS             complement(15567..15833)
                     /locus_tag="IWK15_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9546554.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16088..16813)
                     /locus_tag="IWK15_000935"
     CDS             complement(16088..16813)
                     /locus_tag="IWK15_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9546555.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17174..17244)
                     /locus_tag="IWK15_000936"
     tRNA            complement(17174..17244)
                     /locus_tag="IWK15_000936"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17210..17212),aa:Gly,seq:ccc)
     gene            17361..18113
                     /locus_tag="IWK15_000937"
     CDS             17361..18113
                     /locus_tag="IWK15_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9546556.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18129..18608
                     /locus_tag="IWK15_000938"
     CDS             18129..18608
                     /locus_tag="IWK15_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9546557.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18859..20439)
                     /locus_tag="IWK15_000939"
     CDS             complement(18859..20439)
                     /locus_tag="IWK15_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9546558.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20432..21175)
                     /locus_tag="IWK15_000940"
     CDS             complement(20432..21175)
                     /locus_tag="IWK15_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546559.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21351..22127)
                     /locus_tag="IWK15_000941"
     CDS             complement(21351..22127)
                     /locus_tag="IWK15_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9546560.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22375..23211)
                     /locus_tag="IWK15_000942"
     CDS             complement(22375..23211)
                     /locus_tag="IWK15_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9546561.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23222..24151)
                     /locus_tag="IWK15_000943"
     CDS             complement(23222..24151)
                     /locus_tag="IWK15_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9546562.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24343..24696
                     /locus_tag="IWK15_000944"
     CDS             24343..24696
                     /locus_tag="IWK15_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9546563.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24770..25426)
                     /locus_tag="IWK15_000945"
     CDS             complement(24770..25426)
                     /locus_tag="IWK15_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9546564.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25652..27586
                     /locus_tag="IWK15_000946"
     CDS             25652..27586
                     /locus_tag="IWK15_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9546565.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27672..28895)
                     /locus_tag="IWK15_000947"
     CDS             complement(27672..28895)
                     /locus_tag="IWK15_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9546566.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28889..30787)
                     /locus_tag="IWK15_000948"
     CDS             complement(28889..30787)
                     /locus_tag="IWK15_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9546567.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30781..31899)
                     /locus_tag="IWK15_000949"
     CDS             complement(30781..31899)
                     /locus_tag="IWK15_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9546568.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31982..32815)
                     /locus_tag="IWK15_000950"
     CDS             complement(31982..32815)
                     /locus_tag="IWK15_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9546569.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32808..33725)
                     /locus_tag="IWK15_000951"
     CDS             complement(32808..33725)
                     /locus_tag="IWK15_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546570.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33952..35076)
                     /locus_tag="IWK15_000952"
     CDS             complement(33952..35076)
                     /locus_tag="IWK15_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9546571.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10613 a         7120 c         5756 g        11821 t
ORIGIN      
        1 ctattcttct tcttccgcca tgaatgagaa acccatatca aagaactata aaggttatca
       61 aaacagcgtt tatgactaaa tgaattagag ttatctatca ctatttttca agcgatttaa
      121 accaaatggc atacaatatg gcatacaaaa acttcaataa gccgtggggg tttttaaatt
      181 tcttcttgtt ctttaaaatc aatgagtgga gctacataat ttgaatccac tttttcaaac
      241 gtcacttttt gcatcgacga atgttttctt tcccaagata acgtaaatag atttgactct
      301 ggacttataa agtctacttc accttctagt attactttct tatgcaaata ttcctctaat
      361 aaatatagac ttgctaaagc atacaacaca ttttccaaat ttgcttcctt ataatttgaa
      421 gaacgaccat gttttactct gttatatatt ttccaccaat ctggtgtatc ttcaggccac
      481 ccatctccaa aaggttttag tgaagtcaat gttaaagaat tcataaccct aacaccatta
      541 tccaaacaca ttaattcgct agatttaata acagctttaa aatcagttat gcttttattg
      601 ctactgctat caaatccgca aagttctttg aaaataatct cacactccga accaatcgct
      661 agtagtagtt tttgatattc aatagagaat gttttgaaat tattctcatc gagcatcaca
      721 aaatctgtgg tattaataaa ctcattttct aaatgtagat aatacttcca ataaaaattt
      781 gtaaactctg ctttgtccat taaatcacct cttttgaatt ataacaaaaa aaacacgtcc
      841 acaacagacg tgttctttga tttctacaca atcagcttgc caaaaccatc caatcagtcc
      901 tagcatgcct gtattaaaat tgaacgttca tcatctatca tctttcttag tccagcatgt
      961 ttgcaaccac acctacaatt ttcagtccaa caatagattt cgactagcta agtcttgcaa
     1021 aattcaggca gctaaccctt catttactac cttaattata ctaaataccc acaacgttta
     1081 caagtgtaat cattctaaga aatctgttta gctatgatcg ttgtataatc tttaaacgcc
     1141 gttccttcat tgattgaaat aattttataa ctcttgtttt tccaagctac tttcatatcg
     1201 tttgttagtt cataatcttg atcgtatcga ataataaacg tcaacgttcc ttcaagaatg
     1261 gtacctacac tagcaagcac gtcttttagc atttgtgaac gcacattagc ccaacattca
     1321 aatacagttt cttcactatc caccatctgc ccatcttcat cttttacccg ttttgtcttt
     1381 ttaaacttga ctctctcagt caattcattt acattcttaa tgattggcat aataagcacc
     1441 tctcatttgt tgtactaatg cttgtaccgt tactggtatc cgttcgctgc tggcttcttg
     1501 acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc aaatcaaatt gctgataatt
     1561 atccatatct ttatccgttg cgctactatc aatagcactt tttatatatt cgcctgctgt
     1621 atcaatcaat tgttgaataa agtcatcatc aatagtgtga tcgatccgca tacttttttt
     1681 tatgctatcc aaaattacca ttttatctct cctaatgaaa aaaaggcagt gggataaacc
     1741 gccgcccaat atttttctat acttttggtg ttacaactgg cgtaaactca atatacactg
     1801 aagcattttg atcaattttc ttataatcgt tgcgaacgat cactgcaagt ccttgtgagt
     1861 agtaatcgaa tttttcccat tgagtcgtta cttgatttct acgagcaaca aagatagatt
     1921 gtgcaatatc ccccataatc atagggaatg ttccagcttt aggattggca aacaatgtat
     1981 ctgcaatcaa tactactggc ataccgaata atgatttacc actaggtgct gtcacatcgg
     2041 gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc taggtggtaa tatccgcttt
     2101 ggtttagaat taccatttta tttaatgctg ggtctaatgc cacattgtat agttgtttca
     2161 aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt taataaatcc atgatatgct
     2221 tattgtcagt gttttctacc aatttcgcta actgatcttt gacctcttgt acaatattca
     2281 ctgctgaatc ttccacaacc tcatttgata aggcaatctt accagcacga gtttctactt
     2341 tataatcaac tgaagtaaac atttcagcgt caatatcacc aatttcagca agttcagctt
     2401 ttgttgctaa cactgcttgt tggttagttg ctactggata tttaccttgt ccatttgata
     2461 ctgtttttac tgttgcatat tgagctaagt tataatttga acgtttcaaa tcaaagactt
     2521 caccgattac ttcttcggga acaactgccg ctgcatttac agtagtgact ccatcacgca
     2581 cttcgccttg tgaacggata tattcttcat acccacgcaa ttctgttttt tcgttatcga
     2641 ttaaagtttt ttccatgtta ttactctcct tttgtgcttt agaagtggat tctaagctat
     2701 tgtttgtttg ttttgcttgg ttgttactca taaacgattc gtaagaacgc ttatttactt
     2761 ggacatttga tgaatcataa gcgggaacgg tcacgacact aatttcatta agtgctttga
     2821 ttttatctat tgatcgttca attgtgccat cttctttttt gtcgaatgaa tctaatccaa
     2881 gcacgaaccc aaaggacata gaatcaacaa ccccttttga gatattctca tacacatcat
     2941 ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa tcctgtatca tcgacattca
     3001 atttcaacgt acctgctttg acactagcta gtggctttga ataatcgtgt ccatataata
     3061 agaacacatt tgataaatcc acctctttta atgcttcggg tgtaatcact tctacaaagc
     3121 cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata gcctgaaata gttttgccgt
     3181 ctgttggttg tttttcattt tctttctcta ctttgggaga atcggctgtt agctcagctt
     3241 cttctgtcag ccgtttttcc ttttcttctt cattattcaa ctggtgtaac tccttcctgt
     3301 tcttttaatt gatatttctc caacgtatct aaatacgtaa agttcaaact tgctagtaaa
     3361 cgatcgccac catccatggg gggtagcccc attttcgacc gcccctcatt aatcgtgaga
     3421 agtgaccctt tgacctgatc caatacattt tttacttttg tttcaggatc agtctctagt
     3481 aactgatcgg aattaaagcg gatattggtt tcaagtttcg tatctaattc actcacaaac
     3541 acattaaagt aatgaatcag cgtactttgg atatactgga gattactttg aacggtgcta
     3601 gaatgttcat tttcaacgcc taaacgctca atcggtacac caaacgcctt ggcaatttgt
     3661 tttgtgttcc aatcattgga attgataagc ttcaaaacat cggtgtttat ttctaatgtt
     3721 ttgtagtcca ttgtttcatc aagaatgatc gttctaagag cattatctcc actacttgaa
     3781 ccgttggctt cttcaaactt ttcacgaatt gcgttctttg ctgatccgtc taaatctgac
     3841 ttatgaacct tcaaaatacc tgacccactg acaccacgag taaagaaatt gtgcaatgtg
     3901 cggttgccag cttgttgaat tttcagttca tcacgtaacg cataaagtgg tggtaatcct
     3961 gttaaaccat cttgggtgaa atatttgaag tgcaaaatat cgctagactt cacacgtctt
     4021 tttttatcac caatctgata agacaacgta ccattatcta attgagtaac cgttacactt
     4081 gagttaggta ataagtgaat ttcttctacc ttttcaccgt tgcgtttaat ctctgcaaag
     4141 ctattaccgt ttagcaacat attgaccgcc aaagcaaact ttaaatgcca accatccatt
     4201 tctgaattag gtttctcgtt tagtaacttc accagttcat tatcagcttg tggcatattg
     4261 tttttgacca attgaatcgg acttgaagca atatcactag caataattcg aacggctgca
     4321 aacacatcac tatttcttaa tgcacgaacg cttgtaaagc tggtggtgta atcatccgat
     4381 tggattgata ccacatgttg taggaaagga tctcccgttg tattttgtct cgctttaaaa
     4441 aatgccatct tttaacctcc cttctgttct gtatttagca tgaccgctac cccaataagc
     4501 actatgccca gcgctaacaa gccatagtag acatttgtta gtaaggtgat agcaaccaac
     4561 acacacacca atccaagaag taaaagaacg gtatgaatgt aattagaaag aaaattcttc
     4621 acttgtataa aattcattgt ctgctttcgc tccttccgtt gtggtgaagt aatccattgc
     4681 aaacacatag gcattgatta atgcagctat tggatcaatt ttgttactgt tctttgattt
     4741 attaatctga atgccgttgt tatccgtttt taaaatagcg ttgttcactg catgggtgag
     4801 aatcgtattt ttattgtgaa taacgttgcc ttcataaact tgttctctaa aagttcgggt
     4861 cggaacgttt agagtaatcg ttccttgtct tacttctaac attgggtagt tggctttttc
     4921 agctttactg ataagcgaat tagcgttata cgggtcataa caaatcccca tacattccaa
     4981 atcattttct tgaatcaaat cttgaataaa ctgaaatact tggtcatagt ccacgattcc
     5041 actttctaat tgagtaatgg aacactcacc agctttttct agttctcgat aaggcaaacc
     5101 gtcgcgcttt tctttgtctt gaagcccata tttggtggct acaaagctat gtgaatcaca
     5161 ataaagttcg ccgttatcta gcggtacgat ccatgaaacg ctagttaaat catctgtttt
     5221 agataaatcc actccgatat aaacgggctt gcctgtaata tcttgtggtt ctacttcggt
     5281 tgcctgccag tcttcagcaa tcatatagct gtcctcactc gcttgtctcc ataaattgaa
     5341 gtttttcacc aatacagaat taaggttatt ttgtttcaaa gcaaggttca catcatcttg
     5401 aatggttgga atcattactt ttttgatttc ttcactttca aaaatcggat tggctttgat
     5461 ccatttctct tgatcgtgaa tttcttcggg atcgtccaat tcccatatcg ctataaagta
     5521 cctgtcggct tgttcttcgc cttttagcac acgatctaac aataagtatt cctcgtacat
     5581 aggaacgttt aaatcaagcc cagcggtact aataacacac aataagccgt tcttttgttg
     5641 ggtcattcct gacttaatga cgttgtacgt tttacgagtt tttgcttcgt gccactcgtc
     5701 aagaattgcg gttgttggtg caaacccatc tagcgtgctg gtttcactag ccagtgccat
     5761 agcgaacgaa ttagagggca agtgctgaat cttagagttc ataattttca gttgtggtct
     5821 taaaaactta ctagacttga ccacgtttct aagtgaatta gacagcatat catagcccaa
     5881 tttagcttgt ttcaaagcgt tacttacaaa caatacttgc cttgcttctg ctggttcttg
     5941 ttccataatc aacgcattgg cagccatgcc acttgcgata tacgttttcc cattcttacg
     6001 gctcatactg gtaaacgctc gattgaatcg cctgtaattc ccttctttag tacgccagcc
     6061 ataaagactg cctagaatga atttttgaaa tcctaacatc ttgattgctt taccgtccgt
     6121 agatggtagc aactcaatga atttaatcgc tttggtggct tgttcttcat cgaacacata
     6181 agggaagcta tctgacttag aacgctccaa atcgcttata tgacgctcac acgcttggat
     6241 aattttcttg ggtgcaatca gcttacctga taggactttc tcaatgtaat cattcatgaa
     6301 ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt taaccttgtt caaagctagt
     6361 tttgatcgtg attctaaggt catacctagt gaggtggctt gttttttcaa atcactcata
     6421 gcttgtgatt gaacccgaac cgctggattg gctttcttta ctccgttttc gcatgtgata
     6481 aacgtacctg attttcttat ctcggcttcg gctgttttca ttcgagcaaa ggctaaacaa
     6541 taggctacca atgaaccata atctgcttca ctcaatggat aatctttttt taatgttggt
     6601 acgatacgat tccattcact tctagcgctc gctgccatcc agtcaggggg cgtgatattt
     6661 attaatggtt gctgcttgaa caattcttct ctagcgtcca cacggattgc tttttcttca
     6721 cttgatatat tgccttttgt atcgtgtaat attttagggt gtcccatgtt gtcctccttt
     6781 cagacaaaag aaaaaggaac gtaagcaatt aagctcacat tccttcgttt ttcgatcaga
     6841 atttttcttt atctaattta tctatataaa tgatttttcc gttctgcatt tgaatggtca
     6901 cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc tgttttatca gtaataacaa
     6961 ttgtattttt caataaatct cctactttca ttatatactt tttcacttgt gattacaagt
     7021 gtaatcgtta tttgtaatta cccttatgcc ttttcacaac ggttatttgt accaaaatga
     7081 gtaggcatcg attgtcagta agtttcacac tatgccccct taactcaaag ggtactcttt
     7141 tctcgtcttt gtctttcatt ggctgtcttg gaattatggt gtgcatggca taaagaacga
     7201 aaattagttt catctaaccg tctattccaa tcatctttta gttcaatttt gtgatctaca
     7261 acatcagctt tccttatcaa gccttcttct aggcaatcct cacatactgg atgatttaac
     7321 cgatacaagt atgaagcctt tcgccatgtt ctactttgat agaattgaaa atatttacct
     7381 tcgctttgct ttctttcttc acgcttaatt gccgtttgct ttgctttatg cttcccacaa
     7441 tacttctgcc tataatcaat caatgtttta catccagcat gattacattg ttttttaact
     7501 gccatagatt accaaccaaa gcgatctcgt tcgtcagaat catcttgttc ccattttgat
     7561 ttgtattcaa tataaggcga aactatcttc aagcacgttc cgtctttaaa attaacttta
     7621 gtgattttat cttcctcaaa actaataaag tctacatctt cttgagtgtt tgaatactca
     7681 atgtgtttct caccatcgaa gtatctaata ctttccatat gcataattat tctacctcca
     7741 ataaattagt tatcaattca tcatctgtta gtagcaaagc caagaactta tcatggatgt
     7801 ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg tctcgctcga tcatataggt
     7861 taagcagaca attaaattca tccattagct cacctgtatc atcatagttg ccactatcta
     7921 ccaatgatcc cactactagc gattgacacc acgctactga ctcctgcagc caatcaggtt
     7981 cttcttccat tatctttaat acttctggat caaatgtgga ataatcttct gttaccaatc
     8041 ccacactaaa tgccagcgtg ttcattagaa accgatccat ttcatctgta tattgaaagt
     8101 tcggtgcttt gtttgttgaa ataatattga gcatttactt tcctccttga ttcgtaacac
     8161 gtggtaacac ctacgtaaca ggtacccgtt accactcaat cccaacgggg acaagggttc
     8221 tagcgttttg gtaacaggta acgggtaaaa tccagtaaca ttattcccca gcatattagc
     8281 taactatttc aaaagtttta aggaaataag gtgttacccc gttaccaaat tgctgtatcc
     8341 cttggggcag taagggtaaa ctagtaacgg gctaggtgtt actgacccgt tactacccgt
     8401 tactttttgt actttccagc tcgattttta ttttcatagg catttgactt attgtaaaat
     8461 cctcgtcgtt tatttctttt tggcagttgc ttccgttccc aaccaggaat actgctaata
     8521 atcaaggcaa tctttttagt ttctgcatct gatcgaccat tcaataaatc ctttgcttgt
     8581 ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc tatcaactaa ataaaactct
     8641 tgttgcatag tagctccatt atagaaatgc tgtaaatttt tctcattaca tttactgtca
     8701 ggatagttct gataataaga acgtctcgcc cacgctggag cttcttccca tttatacggg
     8761 atttccattt ccaaatattt agtgattgct tctttaattg gattctccat cttggcatcc
     8821 tcttgatagt cttttgcaat ttctaagact tctttatctt catcgggact aaaatagatt
     8881 cgttgtcctt tatcaaaaag cacctttgct tcagctaaca cttgaagaaa atattcatcc
     8941 tttgttttaa acggattcat ctttggctta ttcttacaag gcaaaggata aaaacggcgg
     9001 tttcctgtat catctttcaa atactgccca tcattcgacg ttccaataaa tacacattgc
     9061 cttgcccaag ctatgacatt ccgaccataa ggaggtctaa ttttatcgat cttggcactg
     9121 atgaagtttt ttatctgttc aattttagtt ttgttcattg aagataattc tccaagttca
     9181 ataattacat tgccttgaag ctgcatataa tcatctttct tttcacctag cgaatctaat
     9241 gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa cggtactctt accaatccct
     9301 tgcggtccat ccaaaactgg aacgatctca aactttattc ctggtttata gatacgtgcg
     9361 actgatccga ctatccaacg ttttgttact tctcgtgtgt aaaggttgtc gtctacccct
     9421 aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat cccatttaac tttttcgata
     9481 cgttctttta ctgggttgaa aacattcttt ctagccacat ttactactgc tttgtgtatt
     9541 ttgttgtcat caaaaactac attgtagttt tcttcaatat atgccgttaa ggcatcctca
     9601 aaactgtctt cccattctcc agcggtacga ttgatccaag gagaatcttt taataggtga
     9661 atgtatccgc taaattcgtt ataagcaatt gttccagcta aattgctatc attggttaaa
     9721 atcaattctg cattcacaac actgttactt ttaatctgat cgtctttatt gcatcttaat
     9781 cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac gctcgctggc ttcgggaaag
     9841 tctaaaatac tatctaattt atgcttatga gccatgagct ttccccctca aatctttttt
     9901 aatcatcgat cgaataattt gattcacttc tttatctttt agcggtggtt cattgtacgc
     9961 atttgctgta taaatcaatt ctgctaaagt ttgattatct acaccgtgcc atagtaacca
    10021 accgcataac ttagttaaat gatcgtttct actaccttcg ccagcgcctt gaacaatcat
    10081 atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg tattgagtct ttatttcttg
    10141 cggttttttt agcagtaggt tcactagcca atcgggagct ggtgcaatat tatttagaga
    10201 tacgttttca ataggttcat atgctctccc aacacttctt gaaataatca tagttggcgc
    10261 tattggaact cctttagtga taatatcaat cccgcttaaa cttttacctt tatcatcacg
    10321 cccgaagata tcagcaaatg cacctgtttc ttgttggata attacaccat caggaatttt
    10381 aaagaagaaa tggattcctt tacctggtgt gatttccgta taagtagaag gaaatggatc
    10441 atacattgaa gcaagttttt tataattctc aacgccgttt cgtgtttcag aatgttgatc
    10501 taggtcaact accaccattt gatgatcttc aagcattaac ccaatatttc tagttgcatc
    10561 ttccgtccac cattcattaa tttgattaga atcgttagtt gcatcattcc aaccactaga
    10621 gccttttatt ggaattttag ttccaggaga taagggataa attgaccatc ccgacttttg
    10681 taattccgtt gctgcttgat aattatttgc caataaacct ctcctccttt ttcaaaatac
    10741 tggtataaat cattcagaca cctccatgag cttcaactgc tcaagttcgt atttcaaccc
    10801 atcccgaaca aattcagcac cttttaattg attctgatac tttacccatg tattaaatgt
    10861 tttcaccctt gcttcttgca tatcagtaat gtttaaccat gcggttaaaa tctgattttt
    10921 tagctcgttg atttctcgac aaattatttt gtgttctgca tgttttttga gtacatacaa
    10981 aactgttagt ttttcattga atgaatttcc aaagtaggtt ttcccgatat tattttctag
    11041 ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta tcgagttggt ttaatagttt
    11101 tgttttgtac atctacttat gccctccagt tcttaattga tagtcgtact cgtcataagc
    11161 agtgaagaga tataacgcta atcctgcaaa gatagctatt tttgcaatta atggtaataa
    11221 gccgaaaatt ccgccgatta aaatgcttcc tataattaag tttcgtttca taatggttcc
    11281 ctccgatttt ttaatatggt ataatcggaa caaagaatat cgggttttat cccaattaag
    11341 ttgcctttat cctgtccgcc aagactgtta tggataaggc tttttctttg ttctcgatca
    11401 tgttaatgct cctaagttgt ctgcaattca gcagaacgat tataaatttt ctcactcact
    11461 gaaaactgca acccatattt ttctttaaca gtcattaatt ctactgtctc atctaaaata
    11521 ggttcacgat caaccaacat ttcaggagtc atatctttct tttttaccat tttggaatga
    11581 ccataaatag ttgatacagc tttattggct atcacattcg ctttaatgta gtctactggt
    11641 tttggttcag taatagcgtg gcttaattta gtcattgctt ctttttggtg ttctttgtct
    11701 agcatgcgga atacttgaaa gccttcaagt cctgtggact ggcgtagttc tttgatgatg
    11761 tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac ggcttttgaa tgccatgcgg
    11821 taaatatttt ttttcgttga tgatgtttac atcttgtgtt ctgcccaatg aatcgatgac
    11881 cttactagta gtaaccccat ctttaggcaa agagtgaatg gctctagtaa cctgtttaag
    11941 acctaacgct tccgcaacat ctttcgccac tgcccaccat tcatcattaa tatcgacaaa
    12001 tcttataatg tgtccgttcc aaatttcagt tttcattcaa cgtcctccag ttcaagccct
    12061 agcaagctcg ctaacaactc aacttgctga tacaaaattt cttgatactc ttcaatagaa
    12121 agtcgttctt taaactctgc ttgtactaaa tcgttcaagc cacgcaatac tccgcttacc
    12181 gttggttctt gtggtagcaa tttttgatag tccatttgtt cgtacaaaca ttcaaacgtt
    12241 tcttctagcg ctttgccttc caagtttgct aattctgccg ctgtgtcttc tgtaagaatt
    12301 gttgataata ctttttcgat tgctttattt tgtgtcgatt ccattattat ttcctcctat
    12361 taatcccttt gcccccacgg cttgcgttta tttttggtat tcttccataa agcgatcaca
    12421 ttcatcttgt gaaattctta cgactccatc aactataatc aactttaaac cgttaggaac
    12481 atatttctgc atcatagtat tgtatgaaac gttcatatac ttagcagctt gtgccttgtt
    12541 catatatttt ggaaactggt actttttttc taataatgaa agtaacgttc cttgagtttg
    12601 ctctacttgt tgaacaactc gatcgtcaat aattttaaaa gctggatcat caagcgtgag
    12661 tttgtcaatt tttaattcca tatttatccc ccctcgttag catacatttc tatacaaaga
    12721 ttttaaaaaa atgcaagagg ctcttgaata catttctata cctcttataa actcaatata
    12781 ccatcatacc ttttcatttg caagaatttt aacatacttt tttgaacttt tatgtttcac
    12841 acatatttaa atcgtgatat agtagagaaa atatatgaat ggtggtgaaa atgtggctag
    12901 aaaaaaatac cccgaagcag tggagacagg acaaagaatt agactggcta gaattgaaaa
    12961 gggactctca atggaacaac ttggaggtaa attatcccca ccggccagta aaggtgccgt
    13021 tagtaattgg gaaaatggct ataatcttcc caataacacc aggcttaaac aactttcaaa
    13081 ggtattagat gtatccacta cctatctatt aaacggatct tatacattaa gagatatgca
    13141 tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa ttgaaaaaaa ctattgaaaa
    13201 aaataactct ataattgatt ctaatctttc taatgctatc aataacatcg aagaagataa
    13261 cctgactgtc tcacagaaga tttatttaac aaacgcaata aaatatctta aaacaggatc
    13321 tcgaattgaa tttcctgacg ggagtacccc aattgcttat ctcgcagctc ttctaaatac
    13381 gcttttacga tatgactcat taattaatga ttcagaaaaa aatacagatg agaaaaaaga
    13441 gtatcaagaa ttttatgaaa atgatcttct aaaagagttc acagctcttg ttactgagct
    13501 aaataaacac tattcaaaat aataataaac tgcctcctcc aaccgccccc acggcttgct
    13561 acactatgga gggaaaacta ttatggcaac attcaagcaa tatgaaacta aaaaaggtaa
    13621 attttggctc tatgaagcct atcttggaat gaacaaaatg actggcaaac ctgataaggt
    13681 tcgtagacga ggttacaaaa caaaaaaaga agcccagctt gccttgtcac gcttgcaagt
    13741 ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa acgtttaaag aagtttatga
    13801 cttgtggcta gaaacatata aaaccactgt ccgagaagcc accttcatga aaactgaaat
    13861 caagtttaga aagtggattc ttccaaaata tggaaatctt cgtgtgaatg aagtcacggt
    13921 aaaaaaagcc caacaaattg taaatagctg ggctgaaact actgaccaat acaaggtact
    13981 tcattcaact tctaagcgta tttttagcta cgctatcaat cttgggatta ttgagactaa
    14041 tccactagac aacgtattaa tgcctaaaag aaccaccact aagcataaag aaaaggtcaa
    14101 agtttacagt aaagaacaac tgatcacctt gtttgattac ctaaacagca aacctgaaac
    14161 ttatcgaaac gactttgata aaacgttact acgctttctc ttttatagcg gggttaggat
    14221 cagtgaagcc cttgcattga attggtcaga cattgatttt caagaaaata cggtcaccat
    14281 caataaaaca ctaagccaat ccaaaaatgg ctataaaatc agtgatccta aaactgattg
    14341 tagtgctggg gttcttccac tagatgaaag aacaatttct atattaaaaa aatggcagat
    14401 taatcagcgg aaatatatgc tgacacttgg tgtaactgat ccgactatga ttttttgtgg
    14461 tatctataaa gaactaatca cacaccacgc tatttatgca agattgatta ctatttgtga
    14521 aaaagctatg gttccatttc tcggtgtgca tgtagcaaga cacactcatg cttcaatgct
    14581 cttggacgct ggagctacta tggtagaagt tcaaactaga ttacggcata caaaaattag
    14641 tcagacgatc gattgttacg gtcacttggc aaaagaaacg aaagaaaaga ctgttgaaaa
    14701 attagtacaa cacttaaata ttgactagat ggcatacaat atggcataca gtaaaaaata
    14761 aaaaaaggag aaaccctatc atatcaaggt ttctcctagc tttatagtct attcttcttc
    14821 ttccgccatg aatgtattga atacttcttc aatcatgtcc cattctgcgt ctgtttcgat
    14881 ttgttctaac tcgccttctg ttccatcttc gttttcgatg tatgcataag cttgtaattc
    14941 tacatcttca tcttctgaaa caccagcagg atataaaaga acatagttac gtccaaattc
    15001 ttcttgtccg tcaactgtta gtaggatttc atataatgtt tcatttcctt ggtcatccac
    15061 caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg ttatgttctg tcataagatt
    15121 acctcttttc ttttattatt aaaataattg cttacctgag ccgtctaaat aattttgtaa
    15181 gatcatcaca gctgctaatt tatcgatgac tttttttcgt ttggctcttg aagtatttgc
    15241 ctgctctact agcatacgtt ctgcctgaac agtggttaat cgttcatctt gataatcaac
    15301 tggcaaggag aatagttctt tgatcttttc tccgtatgac atggaagctt ctgcccttgg
    15361 tccgatggaa ttgttcatat ttttaggtaa tccaactaca aaccggtcta cttcatattg
    15421 tgctactaat tccttcaacc gatcaaagcc aaactcttct tctgcttcat tgatccgaat
    15481 gatctctact ccttgagctg tccagccaaa ggggtcactg atagcaacac ctactgtctt
    15541 agaaccgaca tccaagccca ttactctcat aaattcactc catgatttga gaggtagtcc
    15601 ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc gaatcaaatt tcgagcatca
    15661 cgataacgag gaatgtaggc cgggtctcca gaaagcaagt agccgacaat ctgattgatt
    15721 ggattgtagc ctttttcttc caaagcccga taaacaatcg ctaatgtttc gctcacttct
    15781 tttttgcgac tatcgtcaaa accaaaacgt acggtttcat ctgtaaaacc catgattcta
    15841 cacctctttc ccgcaaggta attccagctt accttctaca accttatttt actcgaagaa
    15901 agggaaaact acaaacaaat attccaaact tccgaataaa ttaagaaaca ctatttgtat
    15961 gtttttttcc ttttttctgt agctttatca aaagtactct tttcataaag tatacgctat
    16021 atttctttga atgtatgttt ttacacaatt ttttcataat attcataaaa aattcgaata
    16081 gctgctacta attttttgtt tttgttgctt gtcctgtctc ataattgatc gtgtagccat
    16141 cttgaacatt gtagataaag acattgaatt caattttttt gtcttcaatg ctttctgctt
    16201 ccaattgcac tcccctggct accagttcat tcccctcaaa atgaggagtg acacggtagc
    16261 gtacatgatg atttgtttct ttgatatatg ccgcaatatc attttcgtga gcaagcatgt
    16321 atggagcatt caacgaacgt gtccctgtca tcaagttttt aatgttgtta ttttgaccag
    16381 tcagctggaa accaattaaa tggctgcgat tatataacca ttgcccgtca cttaatttct
    16441 tttgtttcca acctgtcggg tcaatataca aaggctctct cttttcagta gggaacaaat
    16501 cttttccaag catagcattg gcttgtccta ctctgttcaa tttatcaata tttgaatagc
    16561 tttcccatgt tcctttggaa aggctaagat cctcttttgt aaattccggc tgattcccat
    16621 tgatttctat ttcttgatgt tttccatcat attctggcac atccgctact tgagaagctt
    16681 gtggtgtgga atcaaaaata gttgtaatct gctgaaagat atctgaatct ttttgtgtgg
    16741 tggaaacacc gaatgcccct gcaatcacag cagcgacgat ccccacgatc gccttggttg
    16801 tttttttgct catttcttta ctcctttact aacagctccc taaatcttaa catatgttcc
    16861 cctataaata aatactagaa agaaatagaa acagcaaaaa ataaaacctg ttttaagatg
    16921 gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa tcagtcacca ctctatctgt
    16981 aaggtattta ttattattca aaaattttct tcatactcaa gacaatacac tgatttcaca
    17041 accgcttggt aaacggtgcg caaaagcttg catctgcggt aataagtcca tccaagttaa
    17101 aaacataaga agttgttttt gcttggctgt tcttattact tgttgcagag cagctttttc
    17161 acaacctatt ttaagcgggt gacgggaatc gaacccgcga catcagcttg ggaagctggg
    17221 attttaccac taaactacac ccgcaataca aaaacagtat acgcctcttt tattccttcc
    17281 tcaagttttt ttattcttta cgcttctggt atgttttgta gtaaaataaa actaatcaat
    17341 caattattgg agaagatgct atgacgggaa aagaattaga tctgttgctg gacacttgtt
    17401 tactcgctgg gaaaatcatg atggaaagca atgcggagat gtatcgggta gaagatacga
    17461 tgagtcgaat tgctttagct tctggaaatt tccgtttggt cagctatgtc actcaaaccg
    17521 gtctctttat tggtcttgat cgtacctcga ccatccgaat ggaacaaatt accaaccgtt
    17581 cgattaattt agaaaaagta gtaaaagtaa atgatctttc acgtaaatat gttgcttcag
    17641 agctaacatt agaagaactt tactgcgaac tgcaagcaat cgaccgagac cgttctttct
    17701 ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt atggtgttat
    17761 ttggcggaaa gatgaccgat ttacctgtga ctttagtcat tggaggattg ggatacgcag
    17821 cttatctata tagtttgaaa ttttttagaa tcaaattttt atcggaattt ttatcttctt
    17881 tattgattgg catagccgct atctacagcg tacgttttgg cttaggaagc aatcaggatc
    17941 taatcattat cggatgtgtg atgcctttag ttcctggagt tcaaattacg aacgctatcc
    18001 gtgatttgct agctggacat tatgtctcag gagtttctcg cggaacagaa gcgatgatga
    18061 ctgctacgat gatcggtttt gcgattgctt ttattttcca gcttttttat taggaaagga
    18121 acacaagtat ggggacgata tttattcagt tttcttttag ttttttagcg actgcggcat
    18181 ttgccgttat tacaaatgta cctaggcgtt cattgatttg ttgtggtctt tctggtgctt
    18241 ttggctggat gatctactgg atctgtgttc aaataggcgg atctgctgct tttggttcat
    18301 tgcttggttc tttaggagta gcattcatca gcgatctttt ctctaaacgt ttgaaaatgc
    18361 ccgtaacgat tttcaatatt cccgggatgg ttcctctagt ccctggtggt ttagcttatc
    18421 aagcggttcg aaatctcgtt actgaatcct atcgagaagc ggctcattat gcggtccaag
    18481 caattatgat tgctggagca atcgctttag gtcttgtatt atcagaagtc tttaaccata
    18541 acatccgtaa tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa aaacggcttt
    18601 ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc cttgagcgat
    18661 gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg agcaataaga agtaattatg
    18721 agtattaaaa aaagttttgt aaaaatagct tctcacattt ttacataaaa ttgacttaat
    18781 actgagaaac tgacttttga acaccctcta tctgtaaaaa aggggctgcg acatgatttt
    18841 tgtcacggcc cctcttcttt attcaaattt tttccaaaac gttcgttgat aataaacgat
    18901 ccatagagcg gaaagtatta gaactaaacc gatcacagga atattcacta accaaaatgg
    18961 ataaaacaaa gccaacattc cgtataagac gataagaata atactgatga tcaatgaacc
    19021 catcatagtt gcgactaaac ccaaacttcc tgaacctctg ttaaataatt ggttaatatt
    19081 ggtccagttc agcagcagca accgataatc tctagcgaaa taacgtagtg acaataagta
    19141 gcagccgaat aaagaaccaa atagcaagct agggataaaa aacaaaggca gctgaaaaga
    19201 cagtccgctt aataatgcga tcccaccagt taaaaaactc tgcaatatcc atccgaatct
    19261 gaacttttct tttaaatatt ggttcataga gattggcaaa gaacggataa acagaaaatt
    19321 ttcttgatcc aaagaaataa gattgctgat aaaagaagtc tgattaactg ttaagaaagc
    19381 caatgcgatc cctaccagga aaacaacacc gattaactta ttatctaaat cagttaattt
    19441 tatttcgcct cctagggcaa aagcaagaat aaaaatgatt ggagttaaca aggaagaaga
    19501 aagaacttgc ataatcaaat taggttcttt tactaattga gcattgtaac taaataaaag
    19561 ctgatgcaca ttttggttct ttttatgggt tctttttatt ttgaactcac tgtttgaatc
    19621 agtcagttgc tctgtaagct ttggtactat cagctgtttt acagcaagga aaaatcccag
    19681 agccgtgata gctagtattc cccaacccaa taaaccttgt tgagaaagtg gctgactggc
    19741 agcatcatag aatggcaata atatcgaaat cggatgccga tctgttaact cattttcaat
    19801 agtccccgtt tgatgattca tccacatgat tccagcaaat gcgatcagca tcgagctgcc
    19861 aagcaagata ctagaaactg tttttctatg ttttttaaac actgctgttc gagccaaacc
    19921 aaaaacaatc aaactagcca gacaaaaaag aataatcaaa aatagagaaa acaaaaggat
    19981 tgagataatg atagtaagca aaatccctag atcagctcgc catccggtta aaataaacat
    20041 gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa tctttgctga
    20101 aaaaacctgt tcttgtccaa aaggcagagg caaaaatact tgcaggtcat ttccttcaaa
    20161 aaagatatta taaatgactg atatactttg agttaaacct aacataccaa aaagtgccac
    20221 gtaataagta aagaaacctg gcaatttaac aaagttcata gcaaacatag caaatccgta
    20281 aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac aaaagataga tagttaatcg
    20341 ctttcccttt tttcctttct ctcttgcttt attcgtgagc tgtggattaa catataaaag
    20401 attaactcga attaattcta ataattgtcg cttatacata cggtccatct cctgtcgctt
    20461 gccttccagc cattttcaaa taaatctttt ctagcgtttc atcaggtgag ccattcaata
    20521 attcactaac tgccccctga taaatcaatt cacctttctt caaaatagcc agtttatcgc
    20581 ataactgttg tgcggtatcc aatacatgtg tagaaaaaat gactgttttt ccttttgcgg
    20641 catgagcctt catcatttct ttcaaatcaa atgctgcttg aggatcaagt ccttgcaaag
    20701 gttcatccaa tacccaaata tctggatctg gcagcaacgt gccgatcaaa atcgtttttt
    20761 gacgcattcc atgtgaaaaa cttgcgattg tttcattttg atgactatac atatcaaaaa
    20821 gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt gtctaattca taggcagctg
    20881 aaatcaagtc ccaatattct ccagccgtca attgtaaaaa aatatctggg gaatcaggca
    20941 cataaccgat tttttgtttg atactcaatc gattttccgt caatttcatt ccatctacta
    21001 aaatcgttcc cgatgatggt tcaataatac taactaaact tttaatcgta gttgattttc
    21061 cggcaccatt atgtcctaaa aatccaaaaa tttccccttg tcttatcgag agagacaagt
    21121 cttttaatgc ttgttttttc ccgtaacttt tacttacatt gatgaattca atcatttctg
    21181 tactcctttt gtttgttatt actttttatt gtacccttcg ttattggtgg agtaaatgtc
    21241 aaaaagagtg cataacaaaa aaggatagaa acgactaggc tgctacttgg gttgttctct
    21301 tctacaggaa aacactcttc taacaggtaa gatatgagtc tttgttttct ttaaaatgat
    21361 tcattttcta tcttttcttg cataaaattc tttagtttat caatacttct ctttcccatc
    21421 tctagttgtt ctccatttgc aaatgttact atttcgttta ctctgctaat agatgtaatg
    21481 ttttcagtat taatgatata agcttttaaa tctttacata aatactcata ttcgagtgat
    21541 tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa tactaggcct
    21601 attttatttt tataacctgg catgctcatt agataaagga tttcatcgta atggatatag
    21661 tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga
    21721 tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg
    21781 aattctttta tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta
    21841 ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg
    21901 cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag
    21961 gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg
    22021 atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt
    22081 ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc
    22141 actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata
    22201 aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca
    22261 ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac
    22321 aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa
    22381 agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac
    22441 agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt
    22501 ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac
    22561 tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct
    22621 atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct
    22681 agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt
    22741 ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt
    22801 gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga
    22861 ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac
    22921 tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg
    22981 ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt
    23041 tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga
    23101 aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg
    23161 caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct
    23221 cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa
    23281 taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc
    23341 caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta
    23401 taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa
    23461 tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca
    23521 tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga
    23581 ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct
    23641 cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt
    23701 tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa
    23761 caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa
    23821 aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg
    23881 tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac
    23941 atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag
    24001 gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac
    24061 agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct
    24121 gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat
    24181 tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac
    24241 aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa
    24301 ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac
    24361 ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg
    24421 cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat
    24481 gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa
    24541 gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca
    24601 cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat
    24661 tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg
    24721 attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg
    24781 tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat
    24841 tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa
    24901 agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt
    24961 cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat
    25021 cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat
    25081 caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac
    25141 ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct
    25201 ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc
    25261 caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt
    25321 cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca
    25381 aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac
    25441 gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa
    25501 ttccattgct cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa
    25561 atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga
    25621 ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc
    25681 tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc
    25741 tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc
    25801 tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata
    25861 cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc
    25921 ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga
    25981 aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca
    26041 cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg
    26101 gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg
    26161 cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga
    26221 accaacgaac catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta
    26281 tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga
    26341 agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct
    26401 agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga
    26461 aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg
    26521 agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga
    26581 tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca
    26641 aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga
    26701 tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt
    26761 gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa
    26821 tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct
    26881 agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg
    26941 tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga
    27001 aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga
    27061 tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga
    27121 ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac
    27181 aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta
    27241 tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga
    27301 atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct
    27361 tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga
    27421 atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa
    27481 actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac
    27541 ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa
    27601 cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat
    27661 atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa
    27721 taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa
    27781 acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt
    27841 cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct
    27901 gattctatta attctaataa tccacaaacc atgatcacca tactagatgt atctttttct
    27961 tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga
    28021 ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa
    28081 cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta
    28141 tcactataac cttgatgcgt acttccatat cttggttttc catctttgac atccatataa
    28201 aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga
    28261 gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca atacaataat
    28321 ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct
    28381 ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca
    28441 tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat
    28501 aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg
    28561 ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca
    28621 aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat
    28681 ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt
    28741 ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc
    28801 attaattctt tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc
    28861 actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt
    28921 tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata
    28981 cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc
    29041 cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg
    29101 tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga
    29161 taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta
    29221 ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg
    29281 tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc
    29341 tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa
    29401 tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa
    29461 aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt
    29521 ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt
    29581 ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa
    29641 aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc
    29701 cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg
    29761 ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag
    29821 tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt
    29881 ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa
    29941 aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa
    30001 tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc
    30061 tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta
    30121 cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc
    30181 ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt
    30241 ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt ccgacaatta
    30301 tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat
    30361 gaataatctg gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat
    30421 ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc
    30481 cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca
    30541 accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc
    30601 cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg
    30661 cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt
    30721 aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta
    30781 ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc
    30841 agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc
    30901 aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat
    30961 attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc
    31021 cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat cttttacagc
    31081 aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat
    31141 cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc
    31201 atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc
    31261 aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc
    31321 atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa
    31381 cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc
    31441 aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt
    31501 atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg
    31561 agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac
    31621 gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt
    31681 gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt
    31741 ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat
    31801 atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt
    31861 caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta
    31921 atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt
    31981 tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa
    32041 tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg
    32101 gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt
    32161 tatctgaaag catgactaat ggaaatagat attgattcca acttcccata aagataagta
    32221 aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc
    32281 actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat
    32341 attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat
    32401 aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa
    32461 aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat
    32521 actttgctaa tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta
    32581 cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt
    32641 gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt
    32701 gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac
    32761 caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta
    32821 tcacctccga atttaaattg aatagccgat aatataacag atacaaacac catcgtccag
    32881 gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact
    32941 cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata
    33001 ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt
    33061 agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca
    33121 ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat
    33181 cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt
    33241 aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga
    33301 ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa
    33361 tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag
    33421 taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg
    33481 taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat
    33541 tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa
    33601 atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga
    33661 gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc
    33721 atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg
    33781 agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt
    33841 atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt
    33901 tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc
    33961 aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag
    34021 ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc
    34081 cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc
    34141 ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc
    34201 atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga
    34261 ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat
    34321 catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc
    34381 ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc
    34441 cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac
    34501 ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt
    34561 ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc
    34621 attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt
    34681 gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc
    34741 tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt
    34801 tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat
    34861 atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt
    34921 gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt
    34981 tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt
    35041 tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag
    35101 ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat
    35161 ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt
    35221 ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca
    35281 accaatcctc caatcttgtc aaaaaaacac
//
LOCUS       DACDSR010000018        34330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDSR010000018 DACDSR010000000
VERSION     DACDSR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.018"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWK15_000953"
     CDS             202..1077
                     /locus_tag="IWK15_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546572.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWK15_000954"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWK15_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWK15_000955"
     CDS             complement(1642..2973)
                     /locus_tag="IWK15_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546573.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWK15_000956"
     CDS             complement(3122..3835)
                     /locus_tag="IWK15_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9546574.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWK15_000957"
     CDS             4010..5434
                     /locus_tag="IWK15_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9546575.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWK15_000958"
     CDS             5733..6440
                     /locus_tag="IWK15_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9546576.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWK15_000959"
     CDS             6427..6750
                     /locus_tag="IWK15_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9546577.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWK15_000960"
     CDS             complement(6839..7876)
                     /locus_tag="IWK15_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9546578.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWK15_000961"
     CDS             complement(8197..9180)
                     /locus_tag="IWK15_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9546579.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IWK15_000962"
     CDS             complement(9595..10365)
                     /locus_tag="IWK15_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546580.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IWK15_000963"
     CDS             complement(10358..11293)
                     /locus_tag="IWK15_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546581.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IWK15_000964"
     CDS             complement(11609..13258)
                     /locus_tag="IWK15_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9546582.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IWK15_000965"
     CDS             complement(13375..15978)
                     /locus_tag="IWK15_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9546583.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IWK15_000966"
     CDS             complement(15990..17039)
                     /locus_tag="IWK15_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9546584.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IWK15_000967"
     CDS             complement(17065..17379)
                     /locus_tag="IWK15_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9546585.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IWK15_000968"
     CDS             complement(17395..17841)
                     /locus_tag="IWK15_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9546586.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="IWK15_000969"
     CDS             complement(17842..19818)
                     /locus_tag="IWK15_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9546587.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="IWK15_000970"
     CDS             complement(19976..20872)
                     /locus_tag="IWK15_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9546588.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="IWK15_000971"
     CDS             complement(20865..21545)
                     /locus_tag="IWK15_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9546589.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="IWK15_000972"
     CDS             complement(21546..22328)
                     /locus_tag="IWK15_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9546590.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="IWK15_000973"
     CDS             complement(22321..23238)
                     /locus_tag="IWK15_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9546591.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="IWK15_000974"
     CDS             complement(23402..23725)
                     /locus_tag="IWK15_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9546592.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="IWK15_000975"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="IWK15_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9546593.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="IWK15_000976"
     CDS             27853..28167
                     /locus_tag="IWK15_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9546594.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="IWK15_000977"
     CDS             28180..28554
                     /locus_tag="IWK15_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9546595.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="IWK15_000978"
     CDS             28564..28899
                     /locus_tag="IWK15_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9546596.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="IWK15_000979"
     CDS             28982..30322
                     /locus_tag="IWK15_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9546597.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="IWK15_000980"
     CDS             30400..31074
                     /locus_tag="IWK15_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9546598.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31307..31741
                     /gene="dcm"
                     /locus_tag="IWK15_000981"
     CDS             31307..31741
                     /gene="dcm"
                     /locus_tag="IWK15_000981"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9546599.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            31742..32449
                     /locus_tag="IWK15_000982"
     CDS             31742..32449
                     /locus_tag="IWK15_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9546600.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            32439..32729
                     /locus_tag="IWK15_000983"
     CDS             32439..32729
                     /locus_tag="IWK15_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546601.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="IWK15_000984"
     CDS             32988..34172
                     /locus_tag="IWK15_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9546602.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34187..34306
                     /locus_tag="IWK15_000985"
     CDS             34187..34306
                     /locus_tag="IWK15_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9546603.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DACDSR010000019        33866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDSR010000019 DACDSR010000000
VERSION     DACDSR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.019"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..839
                     /locus_tag="IWK15_000986"
     CDS             <1..839
                     /locus_tag="IWK15_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9546604.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="IWK15_000987"
     CDS             complement(1263..1433)
                     /locus_tag="IWK15_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9546605.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="IWK15_000988"
     CDS             complement(1627..1806)
                     /locus_tag="IWK15_000988"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546606.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2193..3305
                     /locus_tag="IWK15_000989"
     CDS             2193..3305
                     /locus_tag="IWK15_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9546607.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="IWK15_000990"
     CDS             complement(3383..3676)
                     /locus_tag="IWK15_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9546608.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="IWK15_000991"
     CDS             3844..4158
                     /locus_tag="IWK15_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9546609.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="IWK15_000992"
     CDS             4179..6158
                     /locus_tag="IWK15_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9546610.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="IWK15_000993"
     CDS             6171..6497
                     /locus_tag="IWK15_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9546611.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="IWK15_000994"
     CDS             6513..7031
                     /locus_tag="IWK15_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546612.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="IWK15_000995"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="IWK15_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9546613.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="IWK15_000996"
     CDS             complement(8467..9972)
                     /locus_tag="IWK15_000996"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9546614.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="IWK15_000997"
     CDS             complement(10064..10654)
                     /locus_tag="IWK15_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9546615.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="IWK15_000998"
     CDS             10833..12479
                     /locus_tag="IWK15_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9546616.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="IWK15_000999"
     CDS             12528..13247
                     /locus_tag="IWK15_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9546617.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="IWK15_001000"
     CDS             13726..14673
                     /locus_tag="IWK15_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546618.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="IWK15_001001"
     CDS             14685..15605
                     /locus_tag="IWK15_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9546619.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="IWK15_001002"
     CDS             15646..17103
                     /locus_tag="IWK15_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9546620.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="IWK15_001003"
     CDS             17448..18062
                     /locus_tag="IWK15_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9546621.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="IWK15_001004"
     CDS             18055..19779
                     /locus_tag="IWK15_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9546622.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="IWK15_001005"
     CDS             19776..21227
                     /locus_tag="IWK15_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9546623.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="IWK15_001006"
     CDS             complement(21700..23853)
                     /locus_tag="IWK15_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9546624.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="IWK15_001007"
     CDS             complement(23855..24901)
                     /locus_tag="IWK15_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9546625.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="IWK15_001008"
     CDS             25051..26340
                     /locus_tag="IWK15_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9546626.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="IWK15_001009"
     CDS             26353..29055
                     /locus_tag="IWK15_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9546627.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="IWK15_001010"
     CDS             29040..29897
                     /locus_tag="IWK15_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9546628.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30825)
                     /locus_tag="IWK15_001011"
     CDS             complement(29968..30825)
                     /locus_tag="IWK15_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9546629.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31036..33207
                     /locus_tag="IWK15_001012"
     CDS             31036..33207
                     /locus_tag="IWK15_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9546630.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="IWK15_001013"
     CDS             33275..33616
                     /locus_tag="IWK15_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9546631.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT        11022 a         5912 c         6958 g         9974 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaa
//
LOCUS       DACDSR010000020        33817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDSR010000020 DACDSR010000000
VERSION     DACDSR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.020"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IWK15_001014"
     CDS             299..1960
                     /locus_tag="IWK15_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9546632.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IWK15_001015"
     CDS             complement(2030..2377)
                     /locus_tag="IWK15_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9546633.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IWK15_001016"
     CDS             2461..3402
                     /locus_tag="IWK15_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546634.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IWK15_001017"
     CDS             3403..4446
                     /locus_tag="IWK15_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546635.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IWK15_001018"
     CDS             4452..5522
                     /locus_tag="IWK15_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546636.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IWK15_001019"
     CDS             5512..6465
                     /locus_tag="IWK15_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9546637.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IWK15_001020"
     CDS             6477..7487
                     /locus_tag="IWK15_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9546638.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IWK15_001021"
     CDS             complement(7772..8593)
                     /locus_tag="IWK15_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9546639.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IWK15_001022"
     CDS             complement(8690..8968)
                     /locus_tag="IWK15_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546640.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IWK15_001023"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IWK15_001023"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9546641.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IWK15_001024"
     CDS             complement(11724..12311)
                     /locus_tag="IWK15_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546642.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IWK15_001025"
     CDS             12486..13808
                     /locus_tag="IWK15_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9546643.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IWK15_001026"
     CDS             13963..14739
                     /locus_tag="IWK15_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9546644.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IWK15_001027"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IWK15_001027"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9546645.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IWK15_001028"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IWK15_001028"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9546646.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IWK15_001029"
     CDS             16629..17258
                     /locus_tag="IWK15_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546647.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWK15_001030"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IWK15_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9546648.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IWK15_001031"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IWK15_001031"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9546649.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IWK15_001032"
     CDS             19445..19981
                     /locus_tag="IWK15_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9546650.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IWK15_001033"
     CDS             complement(20021..21784)
                     /locus_tag="IWK15_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546651.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IWK15_001034"
     CDS             complement(21787..23502)
                     /locus_tag="IWK15_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546652.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IWK15_001035"
     CDS             complement(24281..24505)
                     /locus_tag="IWK15_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546653.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IWK15_001036"
     CDS             24705..27473
                     /locus_tag="IWK15_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9546654.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IWK15_001037"
     CDS             27554..27973
                     /locus_tag="IWK15_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9546655.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IWK15_001038"
     CDS             27985..28455
                     /locus_tag="IWK15_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9546656.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IWK15_001039"
     CDS             28472..29251
                     /locus_tag="IWK15_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546657.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IWK15_001040"
     CDS             29238..30059
                     /locus_tag="IWK15_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9546658.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IWK15_001041"
     CDS             30076..31125
                     /locus_tag="IWK15_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9546659.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IWK15_001042"
     CDS             31138..32142
                     /locus_tag="IWK15_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9546660.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IWK15_001043"
     CDS             32775..33794
                     /locus_tag="IWK15_001043"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9546661.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11041 a         5991 c         6909 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt gttaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACDSR010000021        32979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDSR010000021 DACDSR010000000
VERSION     DACDSR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.021"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            221..1132
                     /locus_tag="IWK15_001044"
     CDS             221..1132
                     /locus_tag="IWK15_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9546662.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1388..2305)
                     /locus_tag="IWK15_001045"
     CDS             complement(1388..2305)
                     /locus_tag="IWK15_001045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9546663.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2507..2944
                     /gene="zapA"
                     /locus_tag="IWK15_001046"
     CDS             2507..2944
                     /gene="zapA"
                     /locus_tag="IWK15_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9546664.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3002..3549
                     /locus_tag="IWK15_001047"
                     /pseudo
     CDS             3002..3549
                     /locus_tag="IWK15_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            3664..6024
                     /locus_tag="IWK15_001048"
     CDS             3664..6024
                     /locus_tag="IWK15_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9546665.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6111..6425
                     /gene="trxA"
                     /locus_tag="IWK15_001049"
     CDS             6111..6425
                     /gene="trxA"
                     /locus_tag="IWK15_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9546666.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6607..8385
                     /gene="uvrC"
                     /locus_tag="IWK15_001050"
     CDS             6607..8385
                     /gene="uvrC"
                     /locus_tag="IWK15_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9546667.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8530..9429
                     /locus_tag="IWK15_001051"
     CDS             8530..9429
                     /locus_tag="IWK15_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546668.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9422..10684
                     /locus_tag="IWK15_001052"
     CDS             9422..10684
                     /locus_tag="IWK15_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546669.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10847..12745
                     /locus_tag="IWK15_001053"
     CDS             10847..12745
                     /locus_tag="IWK15_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9546670.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13044..13763)
                     /locus_tag="IWK15_001054"
     CDS             complement(13044..13763)
                     /locus_tag="IWK15_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9546671.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            14100..14432
                     /locus_tag="IWK15_001055"
     CDS             14100..14432
                     /locus_tag="IWK15_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546672.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14419..16422
                     /locus_tag="IWK15_001056"
     CDS             14419..16422
                     /locus_tag="IWK15_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9546673.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16441..16911
                     /locus_tag="IWK15_001057"
     CDS             16441..16911
                     /locus_tag="IWK15_001057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9546674.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16932..17516
                     /locus_tag="IWK15_001058"
     CDS             16932..17516
                     /locus_tag="IWK15_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9546675.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17531..18517
                     /locus_tag="IWK15_001059"
     CDS             17531..18517
                     /locus_tag="IWK15_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9546676.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            18507..18818
                     /locus_tag="IWK15_001060"
     CDS             18507..18818
                     /locus_tag="IWK15_001060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9546677.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18840..20621
                     /locus_tag="IWK15_001061"
     CDS             18840..20621
                     /locus_tag="IWK15_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9546678.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20614..21990
                     /locus_tag="IWK15_001062"
     CDS             20614..21990
                     /locus_tag="IWK15_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9546679.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            21997..22632
                     /locus_tag="IWK15_001063"
     CDS             21997..22632
                     /locus_tag="IWK15_001063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9546680.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22662..24017
                     /locus_tag="IWK15_001064"
     CDS             22662..24017
                     /locus_tag="IWK15_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9546681.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24209..25603)
                     /locus_tag="IWK15_001065"
     CDS             complement(24209..25603)
                     /locus_tag="IWK15_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9546682.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25695..26666
                     /locus_tag="IWK15_001066"
     CDS             25695..26666
                     /locus_tag="IWK15_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9546683.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26663..27445
                     /locus_tag="IWK15_001067"
     CDS             26663..27445
                     /locus_tag="IWK15_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9546684.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(27614..27985)
                     /locus_tag="IWK15_001068"
     CDS             complement(27614..27985)
                     /locus_tag="IWK15_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9546685.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            28119..28562
                     /locus_tag="IWK15_001069"
     CDS             28119..28562
                     /locus_tag="IWK15_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9546686.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28605..29414)
                     /gene="yidA"
                     /locus_tag="IWK15_001070"
     CDS             complement(28605..29414)
                     /gene="yidA"
                     /locus_tag="IWK15_001070"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9546687.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29433..30800)
                     /locus_tag="IWK15_001071"
     CDS             complement(29433..30800)
                     /locus_tag="IWK15_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9546688.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30784..31614)
                     /locus_tag="IWK15_001072"
     CDS             complement(30784..31614)
                     /locus_tag="IWK15_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9546689.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31777..32214
                     /locus_tag="IWK15_001073"
     CDS             31777..32214
                     /locus_tag="IWK15_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9546690.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32290..32910
                     /locus_tag="IWK15_001074"
     CDS             32290..32910
                     /locus_tag="IWK15_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9546691.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        10741 a         5659 c         7088 g         9491 t
ORIGIN      
        1 aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga atcaccacga
       61 atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg
      121 ttattttcta cggttatatc ttttacagcc ccttttttgc gtacttaagc tgattttaag
      181 ttttgaccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa taattggagc
      241 ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt ttggtcaaaa
      301 aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga caaaggaatc
      361 atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc gtacactata
      421 tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc tggcggagtt
      481 tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata ctggatattt
      541 agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg aagagctatc
      601 tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg cattaagcaa
      661 tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag taaaaaaggc
      721 gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat tagtacagct
      781 tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca gtttacacgc
      841 agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt actatggaca
      901 agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc tggattcaag
      961 aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt ctattttaat
     1021 cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag acagttatca
     1081 gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt aataaataag
     1141 cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat tatcgcagat
     1201 gcaagctttt gaacaccgtt taaggaagag gttgtgagcc tgccgtttaa ctccgcgtca
     1261 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac ttgtgaccga
     1321 ggagttggca tgagaacacc gtttattcgg aacaagggac tgtgacatga cttttgtcac
     1381 agttccatta tttttttagc agcttcaaag ctttttctgt attggcaaaa tgaagtttgg
     1441 catatttttc aagcataggc atgccgcctt tctccaatag ttttgccgct acttggtcag
     1501 atttcgttcc agctcctgat ggaagggtga ttccagcttc agctgatgct ttgtgtaact
     1561 cttccaaaaa gctggcacga ctgatgatag aagcagcagc aacagctaaa tgatattgtt
     1621 ctcctttagt aacgaagtac agtttttctt gtaattgatt tttttctaaa cgcgcatatt
     1681 ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat cgcttctggt ttttctggct
     1741 cgattttttt tagcagcaaa tggatcgctt gattgtgcaa agcgactttc atccgaacgg
     1801 cattgtattt tggttggatc tcattgtatt tttctggaga taagacaagc agtttatagg
     1861 ggatggtttt ttttaagatg tcagcaagct ggatgatttg gctatctttt aactcttttg
     1921 aatctctaac accaagttta cgtaattctg caagctgatc acgtttcaca taagcagcac
     1981 atactgttag cggaccaaaa tagctgccat ttccgacctc atcactacct aaaacggata
     2041 attgactgat attttttggc aaagaagagg ctgtttttgc tggcttggct tgtttatcct
     2101 gtgcctcccc ccatttatca gcttctattg ctgcattcgt accttgaaac atcacttttc
     2161 cggatgtata agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa
     2221 tctgtttatt taacaaataa gagcgatagt atgctttcat ttgactaagt tggtcgtttg
     2281 ttgctttgat aatgacgttg ctcataaaat tctcctcgtt tttatacata aaactatacc
     2341 gcaactcacg acttttcgtt ttctaagaga ttgcgagaca tagcattttt ttaaagttta
     2401 tcatataata gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga accaacttca
     2461 tggtacacta aataaaactt tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa
     2521 aacacgctac aaagcagtga ttgccaacca aacatatact attatcggac gggaaaccaa
     2581 gcatcatatg gatatcgtaa cgaagctgat caatgaacag ttagcagaat tgaaacaatt
     2641 atcaccacaa atggataatg aacaagcagc aatcttgatg gctgtcaatg cgctttcgga
     2701 tcaattgaag aaacaagaaa gaatcttgga gctggaagaa gaaacagcag aattaaagaa
     2761 aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa
     2821 cgaagctcgg gaagtattga aagaaaatgg tcaagcagat cataccatta aaaatcatgt
     2881 ggaagcacaa cagattttaa acgagaaacg aaaaggacaa attaaacaaa aatcttctaa
     2941 ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt tagaagagaa aggaacgtag
     3001 catgctgagt ttgctgattt tatttttatt gctaattgcg tttttttctg gagcacgtcg
     3061 cgggtttgct ttgcaaatca tattcgcgat tggatatgta ttgtcattta ttgctgccca
     3121 gcatttttac aaaccattag caagtcattt ggaattatac attccatacc cagctgtcac
     3181 cccaacatca aagttagcat tttttgatca ggtatttgct tttcacctag atgaagcttt
     3241 ttatgcagga actgcttttc tgattatttt gctaattggc tggctgctta cccgatttgt
     3301 gggggttttt gtccatggat tgacgtatgt gccgatcttg agacaggtag actggattgc
     3361 aggaggcatc ctgtcactaa tcatggctta cgttactata tttttgattt tgcagttact
     3421 tgcctttgtt ccattagtgt ggtccaaaat caattcggcg agcatagttt cgctcgtttt
     3481 atcgttgaga gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt
     3541 ttgaattaag gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agtttattta
     3601 agaaggctgc gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat
     3661 aaaatgaata accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga
     3721 caatttgtag tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagtgag
     3781 aaacaaacga tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg
     3841 cgaggcggga tccctatacc taaacttgaa aatatccgtc cccatatgaa acggatcgag
     3901 atcggagcag acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca
     3961 agcgaattga atcgtttcat tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg
     4021 tatatgtggg cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca
     4081 atcgatgaag acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag
     4141 aacattcggc gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa
     4201 aatgcaaagt atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca
     4261 gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga
     4321 cagactttat ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac
     4381 caaatcgctg aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt
     4441 ccccatcgtc aagaaatcat ccacaatgct tatgtgattg gtaaaatgga tctgatgaat
     4501 gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat
     4561 catgtcgtct tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat
     4621 gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg
     4681 aaaacgatca cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg
     4741 attcctgcag gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga
     4801 gacgaacagt cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta
     4861 gatattttat caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga
     4921 acagatccgc aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag
     4981 tctgcttatg tgatggcaac aacgcattat cctgaattga aagtttatgg atacaatcgg
     5041 gcaaatacga ttaatgccag catggagttc gatgtggata cactaagtcc aacgtatcgt
     5101 ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt
     5161 gatacagaag ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac
     5221 gaaatgatca cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg
     5281 cgtcattttg tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat
     5341 ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa cgaagtcgtg
     5401 tcagaggcgg aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag
     5461 attgggcaag gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat
     5521 ctacatcaag aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa
     5581 acgttgaaac cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta
     5641 cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa
     5701 gacgagctga cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta
     5761 cgatctgaat ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa
     5821 gaagcgctaa gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa
     5881 gtgactattg tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt
     5941 aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt
     6001 gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa
     6061 gtatagaaat gctatactaa tcatagattt taaaaatgga ggtctttgat atgtcacaag
     6121 taattacaga taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt
     6181 gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag
     6241 aatatgatga agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc
     6301 ctcaacaatt cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag
     6361 tagaaaaagc tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt
     6421 tataaaaact tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt
     6481 ttttttgcgg ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc
     6541 aggctggatt gtttttttga cagacatgct ataataactt taattgaata tgaaggggag
     6601 taccagatga acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt
     6661 tacttgatga aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa
     6721 aatcgtgtcc gctcatattt tactggcagc cataatacga aaacggaacg gcttgttagt
     6781 gaaatcgaag actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag
     6841 atcaatctga taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc
     6901 tatcctttcc ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaagta
     6961 ctaaaagaca aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca
     7021 aagaagatcc tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag
     7081 ccttgtcttt actatcattt gggacaatgt ctgtgtcctt attatttcga cgtagatccg
     7141 aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg acacgaaaaa
     7201 atcgagtcag agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa
     7261 gctgcagaat accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg
     7321 acaaatactg atttactgga tcgagatgta tttggttatg cgattgacaa aggatggatg
     7381 tgtgtccaag tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt
     7441 cctttctata acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa
     7501 aatgagcatt tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta
     7561 gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt
     7621 gaattggcga ataagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga
     7681 aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc
     7741 ccaatacgta tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg
     7801 atggtcgtat tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa
     7861 acagtcaacg gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattca
     7921 cgagtgattc gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga
     7981 caagtagatg tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct
     8041 ggcatggcaa aaaacgataa acataaaaca aatgagttgc tattcggtat agatttggct
     8101 gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa
     8161 gtccatcgat tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc
     8221 cgattagatg aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc
     8281 aaatcgttga aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg
     8341 aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta
     8401 caaagaaagt aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt
     8461 ggtaaataat aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg
     8521 agtgaagaaa tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc
     8581 gatcatgtct ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg
     8641 gctgggaaga caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa
     8701 gtgttatgga atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg
     8761 gaggaacggg gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa
     8821 cttagaggga aatcaagaaa agagattgaa ccaaaaatcg atttttggat ggaaaaattt
     8881 caggtaaaag ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa
     8941 gtccagctga tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc
     9001 agcgggttag atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa
     9061 catggttcat gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat
     9121 catttgatca tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt
     9181 gaatcatttg gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa
     9241 gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta
     9301 gaagatcctg aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct
     9361 atgtttaacc agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc
     9421 gatgcgtaag ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc
     9481 tttcttgatc atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa
     9541 ctttgctcaa caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat
     9601 cagtcagcaa atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga
     9661 agaaaaagca caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac
     9721 tgagaatgat aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca
     9781 actactgata caacaacaac tgactggctt gcaatcgatg cttcgagcaa gtcaattagg
     9841 catttcacca gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt
     9901 caattttgat tcaaaaggaa aaatgacagt tggagaagat aactcgacag tccaatatgt
     9961 cgtaagttat gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat
    10021 tgcacaagag atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac
    10081 gactgcacaa aagcattttt acggaaaact aacaggtgtt ttacttgttg cattaacgca
    10141 aatggcatta tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat
    10201 ggtcaaaaat atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta
    10261 ttctcttcta ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg
    10321 ctctttggtc aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc
    10381 tctaggcgga tatatgcttg gattgatact aggagcttca gatccaaaca atatcgttat
    10441 tcggatcact tcgtatattc cattcttatc ttcatatatt atgccggttc gcttagcaaa
    10501 tgagacagtc ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt
    10561 cttattgatg atcttgtctg caaacatgta caaatcgaat gtactcgtgt atagcgaagg
    10621 cggattatgg agttcattga aacaatcgat ttcaattatg agaaatgaac gaaaaaaagc
    10681 ataaaatata gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg
    10741 tatagaccta tttctatttt tttattatat tggatatata tattcattga aaatagagca
    10801 taacaacttt ataatgttga gtataccaat ttttggaggc gttttaatgg accgctattt
    10861 agatttactt tcagagaagt tccctacaac ggaagatgtc atcacggaaa tcatcaatct
    10921 cgaagcaatt ttgcagcttc caaaaggaac agagttattt ttgagcgata tacatggcga
    10981 atttcctgct tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa
    11041 agaccttttc tctgaacaaa tgacggaaac ggagatcagc caattcacta tttttacggc
    11101 ttatccagaa tatgtgttga cgactgattg gtacaaaaaa caagataaat cccagctgat
    11161 ccatcgtttg atcgatcttt tacggtttac gacagtcaaa tacactcgat caaaagttcg
    11221 aaaaggattg ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgatcg
    11281 tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa caacttgtct tgatgaagga
    11341 agaagaaaaa tttttaacaa aacttgcgca gacgatccaa aaatctgtca tcgatcattt
    11401 gcatatcgtg ggtgatattt ttgatcgagg aactcaagca gccaaagtaa tggatcgaat
    11461 catggatttt tcctctgtag atatccagtg ggggaatcat gatattttgt ggattggtgc
    11521 gtattgcggc tctgaggcat gtttattgac gcttttacgg attgccgcac gctacaacta
    11581 tttatttgaa ttagagaaag aatatggatt aaatttacgt ccattatttt cttttgccca
    11641 tcaaacctat cagaagaatc cagtatttac ccctaaaaac aggaaagatc tatctgaaag
    11701 agagcaagaa gaattagaaa agatccatca agcactttct atcctccaat tcaaatcaga
    11761 acatcagttg ttgaatcgac gacctgaatt taaaatggac gatcgtaaat tgttggaaaa
    11821 aatcaactat gaggaatcaa cgatcgtttt agaaggtcag ctctatgagt tgaaagggac
    11881 ttgttttcaa acgatccagc cagatgatcc taaaaaattc ttggaagagg aacaaaaagt
    11941 gatcgatagt ttgatgtatt cattccagca ctcgttaaga atgcaaaaac acatgacgtt
    12001 tttgatcgaa aaagggggaa tgtatctcac gaacaatcag catgtactgt tccatggctg
    12061 tatacctgta gatgaaaagg ggcagctctt agcatttgaa ttagaccaat cttatcgcgg
    12121 aaaagaactg ctgggatttt ttgaaaaaca gataactgaa agcgcaaaag atttgactgc
    12181 aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg agcgggccat tttcgccttt
    12241 attcggcaag agtaaaatgg caacttttga gcgctatttt ctcacagagc ctcatacgca
    12301 cgtcgaaaaa agcaatccct attatcactt gcgagatgaa gagtggttca gtgaaaaaat
    12361 tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc atcaatggac acacgccagt
    12421 aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga aaagcatttg ttattgacgg
    12481 aggtctatcc aaagcttatc aaaaaacaac cgggattgct ggctactcat tactatgcaa
    12541 ttcttacggt ttccaaatcg tgacacatta tccgttcttg cctattgaac aacaatttgc
    12601 aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag cagcagctcc cacgcaaact
    12661 taatcggaat acgacaaaag gaatggaatt acagcaacag attactcaac tccaaaggct
    12721 gttggattat cgaaaggacg attgagaaaa atgctagcca acattgctca atagactaaa
    12781 aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga attagctcaa aaaacggtaa
    12841 aatattagaa gctatttttc tgtttatttc tctaatactc aggtcagggc gctgatttca
    12901 cgacctcttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca
    12961 acaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc
    13021 tcacgaccta atcagaaaaa cgattaaaat tcattcacaa agaaatcgaa taaacgctgt
    13081 tcagatgaat ctacacgatg agaaagttcc ggatgccatt ggacaccaag gattttgtgc
    13141 atattatctt tactttggac tgcttcaatg agtccatcgt tggaaaaggc gatggctttt
    13201 aacaaaggag aaagatcttt gactgcctga tgatgatagg aattcacaaa gtatgaatca
    13261 gggagaaggt cacttaataa actgtccgaa acgattttga cttcgtgagt tgcaaactgc
    13321 ggggcagtgg gctgttgttc gtgttttacg gaccattttg gatataatga aagatcttga
    13381 taaagtgttc cctccaacac gacattcaat agttgaagtc ctcggcagac tgcaaaaatc
    13441 ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt cgaattggtc tcgttgaata
    13501 tttgtttccg tcagttttgg atgagggtct tccatgtaaa gttgcggact aacatcctga
    13561 ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt gaggcgctga atctggagac
    13621 atgactggca gaataagagg taatccttga gcatctaaaa ctgcatccac aaatccctgc
    13681 ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg ctcgaatcag ttgattcccg
    13741 gcaatgccga tgattggttt catttttatt ccccctaaag tagactaatt gtaacaagga
    13801 tttaaaaaaa tttaaactat gtaagacttg cccctatact tatctgttct tgctgtgtta
    13861 ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat agttgaaaat attgcttaat
    13921 ttgttataat aaaaagtaag cagaaataaa aaggttgggc gtaagcttca aggatctagt
    13981 tcttcaaggg gtgagaagga cattttattg aaggggcctg cctatactaa gttatcttta
    14041 gaggtacata ctttcaatga aaaaagaaag catgtacctc ttttttaaat cgaaaggaag
    14101 tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc agaagaagaa aatgagaagc
    14161 gtgctcgaac cctacggcag cagttgaaga cctatgcaga agaaaaagag caagaattgg
    14221 agataatcca aacgaacagt cggcaacagc tgaaacagtt ggtagaagaa aaagaacaat
    14281 tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc
    14341 aagcaacttt gaaagaccaa tatcaaaagc atcagagtca agcaatcggt gcaatcattg
    14401 aaagggtgaa agaaacttat ggccgtcact aagatggaaa aagtgaccct catttcagat
    14461 aaaagaaatc aggagaggat tttacaagca gtacaaggaa tccaaaatgt tgaaatccgg
    14521 gatctgtatc aaaatagaac aaacaaccag tgggtagaaa aatattttcc agataccacg
    14581 attattgata aagaagcaaa attaagcgct ttaggcaatc ggctgaaaga catccaagaa
    14641 gctatccaat tcatacaaca ccacggcgat agccgtcaaa agaaactcca tttgaaacgt
    14701 caagaacttt ccttacaaga cttggaagca aattattccg aagaaaattt catgaataaa
    14761 ttagaagaag tccttgattt aaaagaaaag tgggaacagc taactgaaaa acgtgatcgt
    14821 ttagaagaag aggaagaatg gcttcttcat tggcaacagc tggatattgc tccaagtatg
    14881 tatgcaagta agacgaccat ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt
    14941 ttcaaggaag aactaagtaa gcttgatgac gtttatatgg aagaaataga ttatacgcca
    15001 aaacaagttt atctttcgta tgttgcttta aaaaaatcag ttgctgctat agaagaaatt
    15061 gctgggaaat acgggttcaa taaagaaacg tatccttatg aacaaaatcc taaagaaaaa
    15121 ttgcaagaaa ccaaacagca attatccatt gttcacgagc agcaaaaaaa gataacgaca
    15181 gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat gggtagaaga agtcacactt
    15241 gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc atgccactta tctgatcgtt
    15301 ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga tgcatgtgtt aaacgaaact
    15361 gtatcagaag aagatatata cgttagtttc gaaacaccaa actcagaaga gattcagcag
    15421 gaagtcccta ctaaattgaa aaaccatccg cttatcgaac cttttgaatt gctgactgaa
    15481 atgtacagtc tgccaaaata tgaagaagta gatccaacgc cttggatgac accattttat
    15541 cttgtcttct tcgggatgat ggtcgcagat gttggttacg gattgctgat gctcattggg
    15601 acaatactag ctcaaaaact atttgtttta ccccgcggga tgaaacgttt cgtaaaattc
    15661 tttgagattc tctcggtacc atcgatcatc tggggattga tctacagttc atttttcgga
    15721 caggcattac caaaagaact atttggtatc cgcttgcctt tcccaatcct atctacgacg
    15781 gatgacgtca atacgatttt gattttatcc gtgattttcg gtctgataca gatcttggtt
    15841 ggattattcg tggcagcaaa ggaaaatatc aggaggaaag attacttgag tgctgtcagt
    15901 gatggttttg cctggcaagg gatcctgatt gggatcgtta ttgcattgat tggtgtcatg
    15961 ttgataaaaa atacagcatt tgtttatctt ggcgcggggc tatcgatcat tgctgcttta
    16021 tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag tcaagggtgc tgccaaagga
    16081 gcgtataatc tttacggatt gactggttat atcggtgatt tagtcagtta tacacgtttg
    16141 atggcactag gtatttcagg cggaagtatt ggcgcggcat tcaatatgct agttgctttc
    16201 atgccgccag ccgctcgatt cagtgtaggg atccttttga tcatcgcttt acacgcactg
    16261 aatatgtttt tgacactgtt aagtgcctat gtccatggtg cacgattgca gtatgtcgaa
    16321 ttttttggaa aattttatac aggaggcgga cgtgcctttg acccgctgaa aacggcggaa
    16381 aaatacgtca acatcaatca taagaaaaaa aacaataaat aattggagga tttttataca
    16441 atgatggatt acttaattag tcaaaatggt ggaatggtat ttgcagtatt agcgatggca
    16501 acagcgacga ttttttcagg gattggttca gctaaaggtg ttggaatgac aggtgaggca
    16561 gcagcagcgt taacaacaag tcaacccgaa aaattcggac aggctttgat tctgcaattg
    16621 cttccaggta cgcaagggtt gtatggattc gttatcgcct ttttgatttt tatcaactta
    16681 ggaaatgata tgtcagttgt acaaggattg aactttctag gcgcttctct gccgattgct
    16741 tttacaggtt tgttttcagg tatcgcccaa gggaaagttg cagcagcagg tatccaaatt
    16801 ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca
    16861 tatgcgattt taggtttcgt tatttccttc ctattagtat tgaatgccta agcataaagg
    16921 aggaaaagca gatgaacgcc attgaaaaaa tcatctctca gatgaatgaa gcagcagagc
    16981 aagaaagagc ggcgttagag caagaagagc gaatgaagat cgatcaaaac tttgaacaaa
    17041 aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga aaaacaaatc gaattacttg
    17101 aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga agtacgacaa gaaaatttga
    17161 atgcgaagca ggaattttta cgacgattgt ttgcagatgc aatagctgaa atggagaatt
    17221 gggatgaatc tgaacaaatc cagtttatca aaaattcatt gtattcatta cctttgacag
    17281 gcaaagtaac attcattgct ggagaaaaat cagcagctta tctttctcag acattgctgg
    17341 atgaatggaa caatgaactt ccctttatga tggttttaag tgatgaaact gttgctgatc
    17401 aagcgggttt tctgataaac gatcaaggag ttcagtataa ttttcttttc agcagccttg
    17461 tacaagatat ccaaggaacg atgagttttg agattgccaa tcaattattc gaataaggag
    17521 ggatcagtgt atggaatacc acgaactaaa tcccttgatt cgcggaagag agcttgaact
    17581 tttgtccaat gagacatttg aacggatgat tcaaacaaat tctgtcgaag cacttggtga
    17641 aatattgaaa tccacgattt acagtccata tatttatgac ggatatgaat atgacttcga
    17701 acagaatctt ttaaaggagc agagccgtct atttcgctgg ttaaaggaat cagcaccgga
    17761 gcctgaaatc gtttggatct atacgatgcg ctatactttc cataatctga aggttttgac
    17821 aaaagccgag atgacaaatc aaaatctgga ccatttgtat atcgacgatg gattttattc
    17881 attagaaacc ttgaaggatg ccattcatac gcaagtttca aatgaactcc ctgaaagcat
    17941 tatggacagt atacgagaag tacgtgatta ttttgaagag tctaccattc ttcagggaat
    18001 tgacgtgatt tatgatcgac agtttttgac agagcagcga cggcttgccg ataagctagg
    18061 atatcaagaa ttgctgacag aagtcattgc ttttattgac ttgaccaata tcacaacgat
    18121 ggctcgagga atattgcaga aaagaacata tgggttcatg acgacggtac tttccagctc
    18181 gggaagtatc gaaaaagaag tatttttgaa ctttgtagat aaagatatgg aagattacac
    18241 gaattttcta ttagagacag attatgctga ttttctgcgc cctgtcatcc gtgagggaaa
    18301 tcttgatctc gtaaagcttg aacagttgaa agatgattat ctaagttcat tgtatcaaga
    18361 agcacaaacc caggctttcg gaccactgcc actactagcc tttttgaacg caaaagaggt
    18421 agaaagcaaa aaccttcgtt tactggttat cggcaaaaga agccatttcg aaccagaaac
    18481 aatcagaaag cgggtgagac aagtctatga cgcataagat cggtgtagtt ggcgacaaag
    18541 attctgtttt gccattcaaa ttgtttggct ttgatgttcg ttatggtaca accaagcaaa
    18601 ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta tggcgtgatt tatgtcacag
    18661 agcaatgtgc agcattgagc ccagaaacga tcgaacgtta caaggaacaa atgatacctg
    18721 ctatcgtatt gattcccaat catcaaggat cattagggat agggctggaa gaaattcaaa
    18781 aaaacgtaga aaaagcagta ggtcaaaata tcttatagta tgaaaaggag atagaaattt
    18841 tgcaaattgg aaaaatcata aaagtctccg gacctcttgt aatggctgag aacatgtctg
    18901 atgccagtat ccaagacatg tgtctagtgg gagatctagg agttatcgga gaaattatcg
    18961 agatgcgtgg cgatgtggcg tctattcaag tatatgaaga gacttctgga attggtccag
    19021 gcgaaccggt tcgctctact ggtgaagctt tatctgttga attaggacca gggatcattt
    19081 cacaaatgtt tgacggaatc caacggccat tagatacctt tatggaaatc acacaaagta
    19141 atttcttagg gcgcggagtt caattgcccg cattggacca tgagaaaaaa tggtggttcg
    19201 aaccaacagt agaagccgga gaaacggtga gtgctggaga tgttatcggg atcgtagaag
    19261 aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa
    19321 caatcgaaaa aggttcattc actattgatg aaacagtatg tgtcattgaa accgaaaatg
    19381 gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag
    19441 aaaaattaaa cccggatgca cccatgatca ctggacagcg agtgattgat acattcttcc
    19501 cagtgacaaa aggaggagca gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg
    19561 ttcagcatca aattgcaaaa tgggccgatg tggacatggt tgtctatgtg ggatgtggcg
    19621 agcgtggaaa tgagatgacg gatgttttaa atgaattccc tgaattgatt gaccctaata
    19681 caggagaatc gttgatggaa cgtacggtat tgattgctaa tacatcaaac atgccagtag
    19741 cagctcgaga agcttctatc tatacgggaa tcacaattgc ggaatatttc cgtgacatgg
    19801 gttatgacgt tgctatcatg gcagactcca cttcacgatg ggcagaagca cttcgtgaga
    19861 tgagcggacg tttggaagaa atgcctggtg atgaaggtta tccagcttat cttggttcac
    19921 ggcttgcgga atactatgaa cgatctggaa gagtcattgc attaggatca gaaggtcgcg
    19981 aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg aggggatatt tcggaaccag
    20041 tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg tcttgattcc agtttagccc
    20101 aaaaaagaca ttttccttcg atcgattgga tacaaagcta ttctctttat tcaacagaag
    20161 tagggaagta tatggatcat atccttcaag ctgactgggc atccatggtc accgagggca
    20221 tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt gcgtttggta gggattgatt
    20281 ccctttcaga taatgaccgt ctgacattag aagtggcaaa atccattcgt gaagattatt
    20341 tgcagcaaaa tgcttttgat gatgtcgata cattcacttc aagagagaaa caattcaaaa
    20401 tgttgaagat gatcttgacc tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt
    20461 atttaagtga gatcatggac ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat
    20521 atatcccgga aacagaatta gaaaaactag atcaaattat tggtgaaatc aagcaaacga
    20581 tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta aagaatatcg tacgatcggt
    20641 gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta
    20701 atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac aagtattgga agtccaagaa
    20761 gataaagcaa tggtccaaat attcgaagga acaagtggta tcaatttacg tgactcttct
    20821 gtacgatttt taggccatcc tttagaacta ggtgtatcgg aagacatgat cggtcgagta
    20881 ttcgatggtt tgggacgacc gaaagataac ggaccagaga tcctgcctga aaagcatttg
    20941 gatattaacg gagaagtaat caatccagta gctcgggatt atccagatga gtttatccaa
    21001 acaggtatct ctgcaatcga tcatttgaat acattagttc gtgggcaaaa attaccagta
    21061 ttttctggct ctggtcttcc tcataaagaa ttagctgctc agatcgcgcg acaagcaacg
    21121 gtactcgatt catcagatga tttcgccgta gtctttgctg ctattggaat cacttttgaa
    21181 gaagctgaat ttttcatgga agattttcgt caaacgggcg caatcgaccg ttctgtcatg
    21241 tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg ctactcctcg tatggctttg
    21301 actgctgcag aatatcttgc ttatgaaaag ggaatgcatg ttttggttat catgacagac
    21361 atgaccaact atgcagaggc attgcgagag atttctgcag cacggcgaga agttccagga
    21421 agaagaggat atcctgggta tctttacacg aacttagcta ctttattcga acgagcagga
    21481 agaatccgcg gactaaaagg atcggttact caaattccga ttctaacaat gccagaagac
    21541 gataaaactc atccaatccc tgacttgact ggttatatta cagaaggaca gatcatcttg
    21601 actagagagc tttacaagag tgggattcag ccaccaattg atgtactccc gtctctttcg
    21661 cggttaaaag ataagggaac tggacccgga aagacaagag aagaccatgc agctacgatg
    21721 aatcagcttt ttgcagccta tgcacaagga aaacaggcaa aagaattagc cgtagtttta
    21781 ggtgaatctg ctttatctga tgtagacaag atctatgcga agtttgctga gcggtttgaa
    21841 aaagaatatg tcaatcaagg attctacacg aaccgttcga ttactgaaac acttgatcta
    21901 gggtgggaac tgctatcgat gttgccaaga accgaattaa aacgaatcaa agacgacatg
    21961 ctggacaaat atctgccaga agggaagtga gttctgatgg cacgattgaa tgtgaatccg
    22021 acccgcatgg aattgactcg attgaaaaaa cagttgacaa ctgccacaag agggcataaa
    22081 ttattaaaag ataagcaaga tgaactgatg cgtcaattca tcctgttgat tcgaaagaac
    22141 aaccagctgc gtcaagaaat ggaagagtct atgacagaag caatgtctga ttttgtattg
    22201 gcaaatgctt cgatcacgga acctttcatc gaagaattat ttgttttgcc ggcggaaaac
    22261 gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa aagtacctgt gatgaatttt
    22321 gaatatgatg aacagctgac agacacacct ttggaatacg ggtatttgaa ttccagtgcc
    22381 gaactggatc ggtcaatcga tcggtttact accctgcttc caaaactttt ggaactgaca
    22441 gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg aaaagacacg tcggcgagtc
    22501 aatgcattag aatatatgac gatccctcaa ttagaagaaa cgatctatta tattcgtatg
    22561 aagctggaag aaaacgagcg cgcagaagta acaaggttga tcaaagtaaa aaatatgggt
    22621 accactgact aagtggtatc tttttttaaa agaggaaaac gatgattaaa caaagcaaac
    22681 aaataaagaa acgtttgccg gctgttcagc ttctcgcatt aggatttttt ctcctgattt
    22741 tagttggcgg cggcctactt accctaccat tttttagtaa tagtgggcat gggacaaatt
    22801 ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt aacgggattg acaacattga
    22861 atacagcaga gcattggaac agtgctggtc aatttttgat catgcttttg attgagatcg
    22921 gcggtcttgg ttttatgatg gttccgattc tatgctttgc attgataaag aagaaagtca
    22981 gtctcagcac acgtattata ttaaaagaag cgctgaatct agaagaagta tcaggtgtga
    23041 tgaagctcat gctttacatc ctaaaattgg cctttgttat ccaattgatt ggtacggttg
    23101 ctttatcttt tgtatttgtg cctgaatttg gctggttgaa aggtatctgg tacagtttgt
    23161 tccatgcaat ctctagtttt tgtaatgcag ggtttgattt gttaggagac agtttagcca
    23221 atcaccagca aaatgtttat ttgatcatgg tggtatctgc tttgatcatt tctggtgggt
    23281 taggcttcat cgtatggcgt gatttactgg aatatcatca gaaaaagaag atgacgattc
    23341 attcaaaaat cgcattaacc gttacaggta cccttttatt aggcggattc gttgtgttct
    23401 ttttcacaga gcgcaatggg gcaacgctag tggaagggaa tatgtttcaa cgattggcaa
    23461 acaccttttt tatgagtgtg acacctagaa cggctggata ttattcagtt gattacctcc
    23521 atatgagcca tgcaggtctg attttaacga tgtttttgat gtacatcggc ggaacatctg
    23581 ggtcgacggc cggcggattg aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt
    23641 cgatgttgaa aggacgtacg agagcggaag catttggccg taccatccga caaggcgcag
    23701 tgttgcgggc acttacttta ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga
    23761 ttttatcggt gactgaaacg atacctaaaa cttctggcat cgaatatatt gcttttgagg
    23821 tcttctctgc gtttggtaca gtaggtctta cgatgggatt gacgccagac ttgactgcaa
    23881 tagggaaatt gatcattata gcgcttatgt acattggacg agttggtgta ttgactgtgg
    23941 tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa
    24001 gtgtcatgat tggataattt catcttctga cagaaaccta gtgatttagt aaaataagaa
    24061 aataaaataa tttttcttgc ttcttggata ctttaagtgc aaaatgttcg tttatttttt
    24121 gaggcactac ccgttgttga acctcgggta gtgcctcttt tttattgaaa ggaatcaata
    24181 agatttgatt cctttattta tcaataggtt ataattcata tttttttatt ttttctttta
    24241 cgatacttag tccagaagct aaaatttcaa ggttattaga ggatagggaa attcgaacga
    24301 atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg ataaagtcgg ataccgttag
    24361 caagaaagtc attctcaatt atcttagggt caatctcagg atcaactgga agaattcgat
    24421 agtaagggac acgattcgtt ggtttttcta ctttgaaaag agaatcaaaa agctgattgg
    24481 ctctttttgt aagacttaat ttagtttcta agatcctatt tgcttcattt gaatggagga
    24541 tttgagtgat gacttccaca tctaatccag aagttttcac atttatatta aaaattgctt
    24601 gttctatctt gctttggaca tgtactggaa ctacaagata agcaactcgc aatccagaac
    24661 aaataaattt tgtcattcca gctaagtaga ttgtttggtt aggtaataaa ttttggaaag
    24721 gaggaatgat cgtatgttga tagtaagttg ttaaaaatga atggatatca tcttcaataa
    24781 cccatagacc ttctttttca atcacttcgg ctaattgctc tcgtcttttg tgagagattt
    24841 gaaaccctat aggattatta caagaaggca taagaaagac accatgaatc ttcttttttc
    24901 tacattcctg aatcaatata tcaggtcgca ttccttgctc gtcaaaatca attggaacga
    24961 tctctaaatg atataactga gccaattcaa tgaaattagc gtatgtatat cggtctgttg
    25021 ctattctatc acctggagag aaaagtgcag aaagtgtgat agccaatcca ttttgaacac
    25081 cggaaacaat agatagagat tctaaggacg gaatgactcc ttgagttttt aaccaatttg
    25141 ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa
    25201 gatttgggtt attactagtt gattgaataa acgggacaac gaatttatta cattcttcaa
    25261 aagaactaac cagaccaaaa tcaatgatat gttcggttac ttcattggta gagatggtga
    25321 tggactcaaa gacattggga gaaataaatg tgccacttcc aatattcgta tacagcaaac
    25381 ctttctcaat tccatattta taagccttgc caacggtagt aaaatttaaa tctaaataat
    25441 cagctagctc tctttgggag ggtaactgag tgtttttctg taatttattc tttaaaatat
    25501 cttgttctaa ttgttgaatc aacgatcgat aataaggacg cttcaatttt tgtttgtctg
    25561 gtcgccaagt taacgggtaa ttgtcataag aattaatagg cattttctcc actccttaaa
    25621 attgtatacc atacaatttt accattgatt gtattgattt tagaagttaa tcttttgtga
    25681 ggagggaatt gtctatgaaa attattaatt ttgaggaagt agtagcttct tttagttttg
    25741 aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc
    25801 aaaatcctcg attggtggaa attttacctg acggaaaagg caaaaatatg tttgcgatga
    25861 tgcctgctta tttgggggag aacagatatt ttggcacaaa agtcattacc gcttttccgg
    25921 ataatcatca gtatcattta ccttctcatt taggagagat tttcttgttt gatgctcaaa
    25981 acggactacc caaagcattg ataaacgcaa atgcagtcac ctggattcga acagctgctg
    26041 tatctgcgct ggcaacagat tacttagcaa atccgcatgc agaaacactt gctttaattg
    26101 gagctggtca acaagcattg tcgcatttga ctgcgatttc taccattcgt cccatcacta
    26161 cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa
    26221 aacagtatcc taaaataacg tttataaatg caatatcagt agaagatgca gtacgtccag
    26281 cggaaatcat ttgtacgttg acctctagta aagaggcatt tcttcaaaaa gagtgggtaa
    26341 gggaagacgc acatatcaat gctgtgggca catttactcc tgatacaaga gagataagta
    26401 catcgttgat ggaaacttct caaatctatg tagatgatta ctcagctgcc ttaacagaaa
    26461 gcggggacct gcttatagcc atggcagaag gagatttaga ggaatcttgt attgttggta
    26521 cattaggcga attagtttct gagaaaaaaa cattttataa aggagaatcg aggccgacga
    26581 ttttcgacgc agttggtcta gctattgaag atctttgttg tgcagaatat atttataaca
    26641 aattagagaa aggggcttta aaatgaactt agattattac gtagttgatg catttgcaga
    26701 tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta gaagaatggc tgccagaagg
    26761 tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa acagcattta cagtgaagaa
    26821 aaaccaagaa tttgagctta gatggtttac gccagatcga gaaatcgatc tttgtggtca
    26881 cgcgacatta gcaacagcat ttgttttatt taactattac aaaatacctg atgaaacgat
    26941 taaattttct actcaaagcg gtaatctatt tgtgacaaga attaaagatt attattacat
    27001 ggattttcct agtattatgc cgaaaaaagt gccgatattg tctgaatatg aagaagcgat
    27061 tggggctaag attaaagaag cctacttagc acgtgattta tttttcgtat tagaagatga
    27121 aaaaacggtg gctaaattgc agccagattt tacagctatc aaaaattttg aattaggaat
    27181 aggagtcatc gtgacagcag aaagtgtgca aaaagacttt gtttctagaa ccttctttcc
    27241 aaaattaaca atcaatgaag atccagtatg tggatcagca cattctaatc ttattcctta
    27301 ttgggcagaa agaatgaata aaacaaaatt gtcagcttat caaatgtcat caaggggcgg
    27361 ggatctagaa tgtgaattaa aagaagatcg tgtgataata ggaggaaaag cagttttgtt
    27421 ttctaaaggt acctcgttta tttgatttct ttcaaatgaa ttaaaaataa attggtggag
    27481 agtggcaacc ttatagctac aatggttgtt aaaaaaagac gcaaactttt tcaatttaat
    27541 gaaaagtttg cgtctcattt taattattgg tgtttgtttt ttattcaatc aagagtaaaa
    27601 tgccccatac taactatcct cggatgactt cgattttata accatcaggg tcaatgacaa
    27661 agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc agtaatctca agaccggcag
    27721 ctttatgttg ctcgtgtaat ttttctagat catcagctgc tatagcgata tgaccatagc
    27781 cgtctcctag atcatacgca ccatgatcat agttgtaagt caactctaat tcatagtcat
    27841 ccccaggaag tgtcaaataa acaagtgtaa atttgttttc tggaaaatca cggcggcggc
    27901 tttcttcaaa accaaatgcc ttttgataaa attcgattga tgcttcaaga tctttcactc
    27961 gaacacatgt gtgagccatt ttcatagatt catctctcct tttcctttta ctcaatcata
    28021 acaaaaagaa gtaaggaaat agttgatttg tgcttgttca tgagaaattt ctcatgtaaa
    28081 atggatagaa aataaaactg ttaaggtgga aactattcat ggataaacca atttttggtg
    28141 aaaaaaagca atttgctgat tatcaaaccc gttatgcagc atatattgtc atagaaagag
    28201 atcagcagat tgcagtaatc gaggctccaa atggtgcttt ttttcttcct ggcggggaaa
    28261 ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa
    28321 ttgtggcaga aatttcttct tatctgggag aagctgatga gtatttttac tcgaattatc
    28381 gtgcaactta ttattataat ccaggatatt tctttgttgc tgattcttgg aagcgtatag
    28441 gagaaccaac tgaaaagacc aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga
    28501 aattgaaacg tggtagccac cgatgggcag tcgagagatg gatcgaaaaa aataaaaact
    28561 gaaagaaagc gagtagatca gctttctctc agttttttta tttattacag tacatatttt
    28621 ttgattgctt cagctacacc gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct
    28681 ttgacaagag aaacggcatt tcccattgct actcctagac cagcatattc aatcatggat
    28741 aagtcgttct cgttatctcc gatagccatg atttcatctt gatcaatacc aaggtgctgt
    28801 gcgagattcg caactgccgc acctttactt gcttctttat ttagtacttc gaagtaatag
    28861 ggcgcacttt tgaccgttgt gtatttatca cggaaagttt gcggtaaacg cgcaattgct
    28921 gcatctaaaa tttctggttc gtcgatcatc atcattttga cgatgttcat gtctggtgtc
    28981 atttcttcag gtgttcgata ttttagcggc atcttgacta aacttgcttc gtggatcgta
    29041 taagcgctga tatctcgatt aggcgtgtag atcgctttgt catcgatcga atggagatga
    29101 ctgccgacac ggcgtgccat cacttcgatc tctaggaaat cttcatgtgt cagtccgtga
    29161 cgggcgatga ttttacctga tttcgtttgt tgtacgagtg ccccgttata agtaataacg
    29221 taatcattat cttgaaaaag gttcaactcg tttaactgtt cctggacacc tggtaaggga
    29281 cggccagtgc ataaaacgat cgaaacacct tttgcaacag cagcggcaat cgcttctttt
    29341 acttcttttg ttacttctct tttctcattt aataaagtac catcgatgtc gatagcgact
    29401 agtttgatag acataatttc ctccaatata ttttaattta tttcaatcaa tgcgccatta
    29461 cggatatggc tggcaaattc ttgataagtt tcgtcaaata aatcataatg atcacgcaag
    29521 gacggatcaa ccatttcttt tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct
    29581 aatgcagcaa caagctgact aacctgtgat aattctatca aagttccgtc atttcgcaaa
    29641 atctcgattt gtgtgcgatg tctgccttgt tctgggcgat aaaaatcata aggtagatcg
    29701 tagctagaat tcacagcagt gtaataaaca ggattatatc cgaccttttc tactaacaaa
    29761 gtgagttctt ggactaattt tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt
    29821 ttacgattca aaaaacgttt agctaaatca tttaaaatag gatcagcaga ttcagaccat
    29881 tgagtaaagt aagtgcccaa gaccccatca tcaagtttga ggtattcttc taaagtaaag
    29941 cttccgtcaa gaaatggcag aagtaattga gaatggagtt caaaactgct ggcatcttct
    30001 tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa ttacttccat accacgggaa
    30061 acaggatgga agtacacttg cacatacatt tgatatcggc taacgatata atcttccact
    30121 gcatgcatgc cactcatgga aaaagcgatc ccgccttcat aaggacgaat cacacgtaat
    30181 attcgggtaa gatcaaatgt tccatattct gttccagtga aataagcatc ccgaagcaaa
    30241 tagtccattc gatctgcatc gatctgacta gaaatcattt ggacaacttg tgggttgggg
    30301 taagtttttt ggatcacact agctaccttt tcaggaaaac cttcttctac tcggttaaga
    30361 atctgataca cttctgtttc tggagaagtg atgatctcta cagtgatggc ttcatgattc
    30421 gtatggaaga tatgttcaaa tgtatgagag taaggaccat gaccgacatc atgcaacagt
    30481 gccgcacata aagtcaccag ccgttcctga tcatcccagc ctccgttgcc tattttttct
    30541 tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg aaatctcata aacgcctaat
    30601 gaatgagtaa aacggctatg ttctgctccg tgaaaagtga aagaagaagt gcctaattgt
    30661 ttaatgcgtc gtagacgttg gacttctttc gaattgatca aatctaagat tacttgatgc
    30721 tggacatgga tatagttatg gactggatca cgaaaaactt tttccatagg cagcatttga
    30781 tatttataag gggttgtcat aatgtagctc ctttatgtta ttacgttgtt ccattctctg
    30841 gatttggttg ttccattgct ccagctggat ttcttttttt agccagtcgg tagaatcctg
    30901 taaatcactt gcagaataca gcttttcaaa agcttttgca aatgcttctt tgacttcttt
    30961 tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc attgtagcag gatccactgc
    31021 aggatagcca tttgtaccaa agtcttcttt taaagcagat tgataaaatc gcttcatgac
    31081 ttgcccgcgt ttttcctgat taccgttgac acttaagtac atcataacag aaatcccatt
    31141 tttgatccga cgctgagcga taccggcaaa ttttttgcca tggatgctta aatcataagt
    31201 tccaggacaa taagaatggg tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta
    31261 tttagtcaat aggagcattt gctcgtaagc gtcatctatg gttatttttt ttgtgtcact
    31321 ttgggggaaa atcaaagaaa tattcaaaat tccttcatct gatacgatcc ctaatccacc
    31381 tgaatttctg atcactggag cgtaacctgc atctttcagt gtttggatcc cgtcacttag
    31441 aaaaggagtt cgaacatcct tcatacctaa gatgactgtg cagtcatatt gccagaaatg
    31501 taaaaagatt tgctggtgtt ctttagaata ttccgttaat atatctgtta atgcaaaagg
    31561 ggaaaagtcg tttttttgat aaatgtgctg atctagtcga atcgcagggt tcattcaacc
    31621 ggctcctttg tataaattca tattttctac aatattatac tctaaaatca aagagttgtt
    31681 ttggaaaaaa ggttttctgt aaaaatggac aagttggttg acaaaacaaa agaaactagg
    31741 aacaatgtag ctatgagaag taaaggagaa tgaataatgg aattatcaca aggaacaccg
    31801 atttcaatca aattgcgtac aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt
    31861 gatttgaaag ggcaaatggt gacaattggc gatacgctgt acatacgtta taaagagatt
    31921 caggaggaga cgggcgatga gattcctgta acgatcaaat tagttccaga tggtcatgta
    31981 cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat ttggctataa agaacggtta
    32041 gaaacatcct atcgaacacc atacggtatg atccagattg ctacttttac gaaagaactg
    32101 catgttagtt tgaaagaccg tcccacagga gggaaggttc gaatcgatta tgatctgttc
    32161 atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg attttacagc gtaaatataa
    32221 aagaaaagtt tgattttttg tgtctgattt tgtatgataa ggagtagact gtgaaaggac
    32281 gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc
    32341 gaagtagcac acgcgatctt agaacaacgt ggcgatgtca tggatttttc tgatttagct
    32401 aaccagatcc aaaactatct tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa
    32461 ttttatactg acttgaatat tgacggcagc tttatctcat taggggataa tcgttggggc
    32521 ttgcgttcat ggtatgctat cgattcgatc gatgaagaag taaaccatgg aatggacgaa
    32581 gaggatgaag atactccacg tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat
    32641 gatgaagacg cgatcgatta caatgacgat gacccagaag atacagatct gacagaagaa
    32701 gatgacgatg atctgtttga tgacgatgat gatgaagatg aagaaatcgc tgcttataat
    32761 tctgacttgc aagaaatcgg agcagatgac ccgacagatg atgaagaaga cttgccagga
    32821 aatatcgaag aagatttaag tatcatagac gaagacgatg atgatgaaga ttatgaagag
    32881 gaagaatttt ctgactctga aaaagaataa acattatttt cttacgagag gttgtgacag
    32941 agacgatcag cctatcagaa taaggaggtg tgacacgat
//
LOCUS       DACDSR010000022        31837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDSR010000022 DACDSR010000000
VERSION     DACDSR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.022"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IWK15_001075"
     CDS             complement(151..2304)
                     /locus_tag="IWK15_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9546692.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="IWK15_001076"
     CDS             complement(2598..3446)
                     /locus_tag="IWK15_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9546693.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWK15_001077"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWK15_001077"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9546694.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="IWK15_001078"
     CDS             complement(5947..6126)
                     /locus_tag="IWK15_001078"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9546695.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWK15_001079"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWK15_001079"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9546696.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="IWK15_001080"
     CDS             8274..10553
                     /locus_tag="IWK15_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9546697.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="IWK15_001081"
     CDS             complement(10646..11293)
                     /locus_tag="IWK15_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546698.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="IWK15_001082"
     CDS             complement(11472..12191)
                     /locus_tag="IWK15_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9546699.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="IWK15_001083"
     CDS             complement(12322..13473)
                     /locus_tag="IWK15_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9546700.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="IWK15_001084"
     CDS             complement(13690..14430)
                     /locus_tag="IWK15_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9546701.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWK15_001085"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWK15_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9546702.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="IWK15_001086"
     CDS             complement(14814..15407)
                     /locus_tag="IWK15_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9546703.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="IWK15_001087"
     CDS             complement(15391..16038)
                     /locus_tag="IWK15_001087"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9546704.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWK15_001088"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWK15_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9546705.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWK15_001089"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWK15_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9546706.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="IWK15_001090"
     CDS             complement(17484..18011)
                     /locus_tag="IWK15_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9546707.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="IWK15_001091"
     CDS             complement(18191..18976)
                     /locus_tag="IWK15_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9546708.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="IWK15_001092"
     CDS             complement(18969..19838)
                     /locus_tag="IWK15_001092"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9546709.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="IWK15_001093"
     CDS             complement(19839..21218)
                     /locus_tag="IWK15_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9546710.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWK15_001094"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWK15_001094"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9546711.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWK15_001095"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWK15_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9546712.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWK15_001096"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWK15_001096"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9546713.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWK15_001097"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWK15_001097"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9546714.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWK15_001098"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWK15_001098"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9546715.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="IWK15_001099"
     CDS             complement(25176..25400)
                     /locus_tag="IWK15_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9546716.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="IWK15_001100"
     CDS             complement(25453..26415)
                     /locus_tag="IWK15_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9546717.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="IWK15_001101"
     CDS             complement(26425..26865)
                     /locus_tag="IWK15_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9546718.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="IWK15_001102"
     CDS             27171..28136
                     /locus_tag="IWK15_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9546719.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="IWK15_001103"
     CDS             complement(28193..28423)
                     /locus_tag="IWK15_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9546720.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="IWK15_001104"
     CDS             complement(28509..29396)
                     /locus_tag="IWK15_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9546721.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="IWK15_001105"
     CDS             complement(29485..30708)
                     /locus_tag="IWK15_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9546722.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="IWK15_001106"
     CDS             complement(30725..31804)
                     /locus_tag="IWK15_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9546723.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10585 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agcccta
//
LOCUS       DACDSR010000023        30624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDSR010000023 DACDSR010000000
VERSION     DACDSR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.023"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..262)
                     /locus_tag="IWK15_001107"
     CDS             complement(<1..262)
                     /locus_tag="IWK15_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9546724.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTA"
     gene            complement(264..461)
                     /locus_tag="IWK15_001108"
     CDS             complement(264..461)
                     /locus_tag="IWK15_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9546725.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(855..2582)
                     /locus_tag="IWK15_001109"
     CDS             complement(855..2582)
                     /locus_tag="IWK15_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9546726.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2575..3768)
                     /locus_tag="IWK15_001110"
     CDS             complement(2575..3768)
                     /locus_tag="IWK15_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9546727.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3955..4599)
                     /locus_tag="IWK15_001111"
     CDS             complement(3955..4599)
                     /locus_tag="IWK15_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9546728.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4693..4827)
                     /locus_tag="IWK15_001112"
     CDS             complement(4693..4827)
                     /locus_tag="IWK15_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9546729.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="IWK15_001113"
     CDS             complement(4829..6961)
                     /gene="feoB"
                     /locus_tag="IWK15_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9546730.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6958..7431)
                     /locus_tag="IWK15_001114"
     CDS             complement(6958..7431)
                     /locus_tag="IWK15_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9546731.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7832..8056
                     /gene="nrdH"
                     /locus_tag="IWK15_001115"
     CDS             7832..8056
                     /gene="nrdH"
                     /locus_tag="IWK15_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9546732.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8215..10374
                     /gene="nrdE"
                     /locus_tag="IWK15_001116"
     CDS             8215..10374
                     /gene="nrdE"
                     /locus_tag="IWK15_001116"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9546733.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10424..11389
                     /gene="nrdF"
                     /locus_tag="IWK15_001117"
     CDS             10424..11389
                     /gene="nrdF"
                     /locus_tag="IWK15_001117"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9546734.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11478..12617)
                     /locus_tag="IWK15_001118"
     CDS             complement(11478..12617)
                     /locus_tag="IWK15_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9546735.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12926..13639)
                     /locus_tag="IWK15_001119"
     CDS             complement(12926..13639)
                     /locus_tag="IWK15_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9546736.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="IWK15_001120"
     CDS             complement(13893..14594)
                     /gene="nagB"
                     /locus_tag="IWK15_001120"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9546737.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14828..16360
                     /locus_tag="IWK15_001121"
     CDS             14828..16360
                     /locus_tag="IWK15_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9546738.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16551..17753)
                     /locus_tag="IWK15_001122"
     CDS             complement(16551..17753)
                     /locus_tag="IWK15_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9546739.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17835..18524)
                     /locus_tag="IWK15_001123"
     CDS             complement(17835..18524)
                     /locus_tag="IWK15_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9546740.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18630..18998)
                     /gene="rplL"
                     /locus_tag="IWK15_001124"
     CDS             complement(18630..18998)
                     /gene="rplL"
                     /locus_tag="IWK15_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9546741.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19051..19554)
                     /locus_tag="IWK15_001125"
     CDS             complement(19051..19554)
                     /locus_tag="IWK15_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9546742.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19964..20653)
                     /gene="rplA"
                     /locus_tag="IWK15_001126"
     CDS             complement(19964..20653)
                     /gene="rplA"
                     /locus_tag="IWK15_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9546743.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20757..21179)
                     /gene="rplK"
                     /locus_tag="IWK15_001127"
     CDS             complement(20757..21179)
                     /gene="rplK"
                     /locus_tag="IWK15_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9546744.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21522..22190
                     /gene="sdaAB"
                     /locus_tag="IWK15_001128"
     CDS             21522..22190
                     /gene="sdaAB"
                     /locus_tag="IWK15_001128"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9546745.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22203..23075
                     /gene="sdaAA"
                     /locus_tag="IWK15_001129"
     CDS             22203..23075
                     /gene="sdaAA"
                     /locus_tag="IWK15_001129"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9546746.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23166..24185)
                     /locus_tag="IWK15_001130"
     CDS             complement(23166..24185)
                     /locus_tag="IWK15_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9546747.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24358..25245)
                     /locus_tag="IWK15_001131"
     CDS             complement(24358..25245)
                     /locus_tag="IWK15_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9546748.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25322..25867)
                     /gene="nusG"
                     /locus_tag="IWK15_001132"
     CDS             complement(25322..25867)
                     /gene="nusG"
                     /locus_tag="IWK15_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9546749.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25968..26138)
                     /gene="secE"
                     /locus_tag="IWK15_001133"
     CDS             complement(25968..26138)
                     /gene="secE"
                     /locus_tag="IWK15_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9546750.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26162..26314)
                     /gene="rpmG"
                     /locus_tag="IWK15_001134"
     CDS             complement(26162..26314)
                     /gene="rpmG"
                     /locus_tag="IWK15_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9546751.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26476..27120
                     /locus_tag="IWK15_001135"
     CDS             26476..27120
                     /locus_tag="IWK15_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9546752.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27176..27679)
                     /locus_tag="IWK15_001136"
     CDS             complement(27176..27679)
                     /locus_tag="IWK15_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9546753.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27692..27738)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28276..29241
                     /locus_tag="IWK15_001137"
     CDS             28276..29241
                     /locus_tag="IWK15_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9546754.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29340..30512)
                     /locus_tag="IWK15_001138"
     CDS             complement(29340..30512)
                     /locus_tag="IWK15_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546755.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30499..>30624)
                     /locus_tag="IWK15_001139"
     CDS             complement(30499..>30624)
                     /locus_tag="IWK15_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546756.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9345 a         6286 c         5415 g         9578 t
ORIGIN      
        1 cagcagttct tccaatttca ctcaaatttc ttattaattc ctcttgatat tcagtgaatc
       61 ttattttatt agtataagca gtaaaatata gataattcac caatctattt attaaaggac
      121 cgggatctaa ttcatatttt tgtttttcta attttaaata taccttttgt aaaacctcaa
      181 gaatatcaga atatttagga tctttactta tagatatatc attatataat tcatgaacaa
      241 tttgcttagc attttttttc attttagcac ttccctggaa ttgctccacc taaaaaacca
      301 cctatagaca ttcctgcaat acaagtagtt gccttggccc aatcgaccgt acatttattt
      361 ttagtgcaat acactccatt tccataatat tttccactat gagtggtacc cccatagata
      421 agttgtgtct ctttaataga caaaattttt aaatgtttca taatctaatc acccctaaat
      481 taatgttttt agactattat tctaacattc attaaatttc aaaaaacaat tccttcttga
      541 aacgtagcaa aataattcct gaataagaat ttaatcactt ttaagtatat aaactatgta
      601 tattctttaa tattttttga gaatgcactt cgtacataat aaactttaat cacattagtc
      661 tttcatacct acgatcgatc tctttgactc gcacccataa attcataata gtttgtcggc
      721 gtctttggac gtttcgaaga tgagctacgc attctctttt tttatagaca aaaaagatag
      781 agacgaacat ggagcttcca aatttggata tttgatccaa ttttggaagc tccatttatt
      841 tcgtgtctgt ctttttattt tttccagctt tcgactaatt cttggttctc tttgatccag
      901 tcttttgctg cttgctgtgg atcttttcca ttgttgattt ccaacataac tttttccata
      961 tctttttctg accaatggaa attgtctaat actttgtaag cttctggctg gtcttctttc
     1021 aatccttttc gaaccatcgt atggatctgc tcttcgcttc ccattccgtt ttcaggatct
     1081 tctaagtatt tcagatcata tttggcaaac atccaatgag gtgtccatcc tgtaactaca
     1141 attggttcgt gttttttgat cgcttgacct agtgcaacgg tcattgctcc tgaagaagat
     1201 gtctctactt tccagtcttt caagttgtca tatttttgga tcgtgttttc agcagccgta
     1261 actacaccag ctcctggctc gattcctatg attttcttac ctgcctgatc ggttaactca
     1321 tcgatcgaat ttacatccat gtaggctggc actgctaaac caacttttgc tcctgtcaaa
     1381 ttggcaccta ggtcttcgac ttgatcttta tattgtgcat attgtgaacc atgagtttta
     1441 ggtagccaag ctgaaaccat ggcatctgac tcgccatttg aaacagattt ccacatgatt
     1501 gaattatcta atggcgtcat cgttacatca tagcccattt gtttcaatac ttcaccgact
     1561 acatttgttg aagcaacttc tgtatcccat tcaacataag ataagttgat acgttttgtt
     1621 tctttggcag atgaaaaact ccctatacca atcgcaccta agatcactac tactacagca
     1681 ccaatgatca gtccttgacg ttttttactt ttctgtgttg ttgctcctgc agctttttta
     1741 ttgtttggct ggttcaaatg ctgtgtaaaa cggtcaatga tgattgctaa gataactaat
     1801 gcaaccccgt tgacaaatcc attcccgact tgtgctcttt gcaatgctga taatacgccg
     1861 cgtcctaagc cgggtgcacc gatcatagag gcaataacga ccattgaaag tgacagcatt
     1921 gttgtttgat tgatccctgc catgatcgta cttttagcta aaggcaattc tagtttgaat
     1981 agtttttgac gacttgtgct cccgaatgaa tcagaagctt ctactaattc tttaggaact
     2041 tgtcgaatcc ctaaattcgt aaaacgaacg gttggtggca gagcaaagat gactgaggca
     2101 aatacccctg gaaccatccc gataccgaaa aatgcaactg ctggaatcaa ataaacgaat
     2161 cctggcattg tctgcataaa gtcaaggatc ggtttgataa tactttgggc tttactgctt
     2221 tttgccatca agatccctaa tggtacccca atgatgatcg agatcacact agataaaaga
     2281 acaagtgtga cagtattcat caaatcattc catagacctt ggttataaat aaaccataga
     2341 ccgattaatg tgaacaacgt aagtcctgga cgtttcttag aaataaagaa agctgctacc
     2401 gtcaataaca agataaacaa aggagctgga atcactaaca gtaaatttgt aatccccgac
     2461 atcactgcct gtccaatcgt ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac
     2521 cattctgtga tattttctac ccagtttgcc actggtaatt gatagttcaa tagattattc
     2581 atgttcactc acctcgcttg tttctgctaa tgcttcaatc acactacctc ggatgacgac
     2641 accttttaat tttccatcct ctacaactgc aagtggagtc ggtgagtcat aaataatatt
     2701 gaaaatatca cttaccacca tgtctttccc aatcaccgtc acattttcat ctaggaagtc
     2761 gatcaatgga cgtttttgct tcctagcttc taacgcttga tctgcagtga tgatcccttt
     2821 caggtgtctc tttctgtcaa cagccatcag catgctgact tcttcattac gcatccttgt
     2881 caatgcaacg tttggtccat cagattcgat attcgtcgtc aatgcaggta ccatgatgtt
     2941 ttgagccgta agcactttgg aacgatcgac ttcttccaca aattcacgta cataatcatt
     3001 tgctggattg gtcaatattt cttcacctgt tccgatctgc atgatttcgc cgtctttcat
     3061 caaggcgatt ctgtccccaa tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat
     3121 cgttttttga acattggctt gcaggtccag taattcgtct tgcatttctc gccggatcag
     3181 cggatcaaga gccgagaacg cttcgtccat cagcaagatc tcaggatcat ttgctaaagc
     3241 tctggctagg ccaacccgtt gttgcatccc accagataac tgactaggat attggtcttt
     3301 aaatgaaagc aaactagaat tttctaatgc tttttctgct ttttcttgtc gttcttcttt
     3361 aggtacacca cggacttcta acccgtattc tgtattttcc aagatcgttc gatgtggaaa
     3421 cagaccgaag ttttggaaaa ccatattgat tttatgacga cggacttctc gcaattcttc
     3481 tttgcttaac ttcgatacat cttctccatc aatatagata ctgcccgaag ttggctcaat
     3541 cagacgattc aatagacgga tcaatgtgga tttcccactc ccggaaagcc ccatgatcac
     3601 gaagatttct ccttctttta cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt
     3661 tttctttaaa atctccgttt tcggctggtg atcattcatc atatctaaag cggcttgtgt
     3721 ctttttacca aatatctttg ttaaatgttt tacttctact tttaccaaac aaattctcct
     3781 tttttatgta aattcatttt tctttctgaa aaaagtgacc acactatgtt aacatcatgt
     3841 agtcactttg tcaaaaatga aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa
     3901 tctttgtaaa taaaaggttt agccaagtta atattttttt aatatttcac acaatcattc
     3961 tgcaagctga tgctccgggt aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc
     4021 aataaaatga acgatgtccc ctgccgtaaa tttagcatag gggccaggtg aaacaagcag
     4081 ttcttcatta tgagaaattg cgataactgt ggcgccagtc gcctgccaga tattcaactc
     4141 gctaattgaa cggtccagat gcattgcttt ttctgataaa gataattcaa acggaagtaa
     4201 aggattcatc ttatgtattc gcttcgtttg attgaccagc tgatccaata attgagaaaa
     4261 atgattcagc tcatcttgtt gcttctccac acttgccatg atttctgctt ttgtatcttg
     4321 gatggattgt acatcttgat attgcaatag gaaatctttt gctttttcct tcgaagcaac
     4381 aaatgcccca cttccatgcc gtacttccat gattccaaga tctaccagca cattgatagc
     4441 cttgcgggta gtctcaggag aagcattgta cgtactggca agtgttgagc gggcatggat
     4501 cttctcaccc actttatatc ggttttcaac gatacgttcg gctaaatcaa cagcaatctg
     4561 ctggtagcgc gggcggacga tttttttctt tccagacatt ttctttcctc tttctttttt
     4621 tactttccct catcttacta tgctctagca aaataagcaa aaaaggggag aatagcctct
     4681 tgcaacgact gatcatcgct tgatctgttc cttttttact gggcaagctg tttgacagtc
     4741 atcacaattc ttaccggatt ttaaacgagt atagacaatt gcgcctgcac ttccaaagat
     4801 taacacactt aataagaatg tcgccatcct attcctctcc ttctaatgac aatatagtat
     4861 aaaactggtt cttggatttt ggctgacgga acaaaacata aacgagtcct atcatcagta
     4921 aaatagcaag atacgtccct aacgctacct gtccaccctc caataagtgt cccaactgat
     4981 aaatcacaaa actaactaaa taagctaaac cgcattgata gcctatggcg atccaagtcc
     5041 atttcttggc attcatttcc cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa
     5101 gtaaattgaa ggctaacaat gaataagctg ctgcgggagt atatgtgtgt tgtagcactt
     5161 gccagatttc tctgccattt tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc
     5221 caataatatt ttctttagcg accaatcctg taatcgctgc gactgttcct tgccagtttc
     5281 cccaaccaag cggctgaaaa acaactgcca ggattcgccc gaaaaaggct aagatacttt
     5341 ggtcttcaga aacaggttga gcataaaaat tataagaaga gctgaaccag atcacgatac
     5401 tagaaacaaa gataatcgtt cctgcttttt taacaaaaga cttgctgcgg tcataggttt
     5461 gtcttaggac cgctgaccat tgaggaaaat gatatgcggg caattccatg ataaaaggag
     5521 ctgtatctcc agcaaacaaa cgtgtttttt tcaagctgat tccagagaaa ataattgcag
     5581 tcataccaag aaaataggca gaaggagcta cccaagattg atgaggaaaa aaggcccctg
     5641 ctactaaggc aataatcggc aactttgcag aacaaggcat gaaggttgtg accatgatcg
     5701 tcaaacgacg atcttgctcg ttttcaatcg tgcgactggc catgacgcct ggcactccac
     5761 atcctgtagc aatcagcata ggaatgaagg attttcctga aagaccgaat ttcctgaaaa
     5821 gtcgatccat gacaaaagcg atcctcgcca tatatccgca gtcttccaga aagctcaagc
     5881 ataaaaacaa tataatcaat tgcggtagaa atcctaacac tgctcccacg cctgcgatga
     5941 tgccgttcaa gatcagctcc tgcatccatc ccgctacttg ccattcagct aacaatctgc
     6001 caacatgttc tggtaaccat gtaccaaaca atgtgtcatt gatccaatct gtccccattg
     6061 ttcctactgt ttgaatagcc agataataga tcagccacat cacaaatgcg aagatcggca
     6121 aggctaacca acgattcgtt gcaatctgat cgattttgtc actcatcgat aattggaaag
     6181 aatcatttga cacaacacag agtgtacata gttgggtaat aaattcataa cgttcattga
     6241 ccagaatcgt ctcactatcg tcgtccaata gtttctctgt taactggatc agttgctcga
     6301 tctctttttg ctgagaaact gctatctcgt aatcctctct taccttctga tctcgttcaa
     6361 aatatttcat tgcataccag cgtttcttgc cgctttgttc tcctagtaca tcttcaattt
     6421 catgtaaagc tgcttctaaa cgtggatcat atgtacaggg aacaggaata gaaggccgct
     6481 tttttgcaga ctcctgaatc ttttcgatta gtttttcgat tccttgcttt ttcaatacgc
     6541 tgattggtaa aactggtaca ccaagaccat aggaaagctt ttctagattg attttttttc
     6601 cttctttttt gatgacatcc atcatattca gaccaatgat cataggaatg tctaaatcca
     6661 ataactgcgt agttaaataa agatttcgtt ctaaattggt agcatcaata atgttgatga
     6721 tcagttcagg ctgttcctga accaaataat ctcttgcaat cacttcttct ggtgtataag
     6781 gagacaaaga atagatgccg ggtaggtcct gaatatgaat gttcggattt tttttcaacg
     6841 atccttcttt tttttctact gttacgcccg gccaattccc tacatattga ttcgaaccag
     6901 tcaagagatt gaacaagcta gttttcccgc tgtttggatt tccgactaac gcgatattca
     6961 ttctttttcc tccctttgta tcaagattag ttcggcctct gactttcgta acgtcagttc
     7021 ataccctcgc actgtcagtt cgattggatc tcccagaggg gctagttttc ggataactat
     7081 ttttacattt cgcgtcaatc ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg
     7141 gattgctacg acgatcccag tctctcctgc ttttaattga tcaagagaag tccagctttc
     7201 agaggaagca gtctttttat tgacaaagat ttgacctagt atttcccgac ttactgccac
     7261 tcgatgactt tttaccagta aaatggcatt gttgcctgat aaattgacga cgacgatttt
     7321 acttccggca acgatcccga tattttgcaa atggcgtttt gttttttctt ctgtttcgat
     7381 tcgttcgact tgataaacga aattctgctt tgcctgcgct aaggtctgca tattgattcc
     7441 tctcttgtga aataaatcat gtattaaaat cattcattat atgttctaat tttcttacgt
     7501 gttacgcaaa taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca
     7561 attatacttg tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa
     7621 tatttcttca tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat
     7681 acttgttttc agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca
     7741 caatatgtag tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga
     7801 taataagaat gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa
     7861 ttgtatgcaa tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga
     7921 agtcaatatc gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag
     7981 tgttcctgtc attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg
     8041 taaacttgcc agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct
     8101 tgagacagca ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat
     8161 gcaatgattt gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt
     8221 cttaaaaatt tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc
     8281 aatggtcaaa tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat
     8341 gtcaaaccaa atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat
     8401 caagaatata tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta
     8461 tatcaatttt tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt
     8521 tattctcaat atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat
     8581 cgagtagcct tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt
     8641 gcagacgaaa tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga
     8701 cgtaaacgtc ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac
     8761 tctatcgggc gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg
     8821 attactttat caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca
     8881 agtggtgtcg ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta
     8941 ggtcagcgtc aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct
     9001 ttcctttcag caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt
     9061 ggcgtgatcg tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta
     9121 tttagtccat atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca
     9181 gctgagtatg atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt
     9241 gatcttgaaa atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat
     9301 attgatacag cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt
     9361 tcagagattt tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta
     9421 ttaggaacgg atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc
     9481 cctaactttg gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca
     9541 tctgatatcg atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga
     9601 ttaggtgcca tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag
     9661 gattcattag actttacaaa tatttacttc atgctgttga attattggac acttgttgaa
     9721 agcaacaata tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat
     9781 gctgacggca cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa
     9841 gtcaaagcat tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa
     9901 caagcagtaa tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc
     9961 tcaatttcat atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa
    10021 gaacgccaag agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag
    10081 acgattccgt attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat
    10141 gcaatggcac aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa
    10201 atcccagaag gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat
    10261 attttaagac attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt
    10321 actgatgatg cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat
    10381 agatagattc gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta
    10441 tgaagcaata aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct
    10501 gacagagcaa ttttggctag atacacgtat cccactttca aatgacctag atgattggcg
    10561 tacattgtct caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct
    10621 agatactgtc caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca
    10681 tgaagaagcc gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc
    10741 ttctattttt agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa
    10801 tacgaatcct tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac
    10861 accgctagaa aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt
    10921 ttatactcct ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa
    10981 actaatcatc cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg
    11041 attcaacgag ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct
    11101 ttatgagctt tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg
    11161 gacagaagaa gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg
    11221 gatggatcca ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat
    11281 ttcaacaggc acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg
    11341 tcatgtagaa gcgatgaaag acgacgatta tttgattgga ttggaataag gatttatttt
    11401 aattcaaaaa tttttcgaaa aataaaaaga gtttagcgta cagatatttt aaatgtacac
    11461 tgaactcttt tttatgctta ttcacttgtt tcttccagca cttttggact gtctaacttt
    11521 tccgaaacat gttccattct tctcaataaa tagattttcc ggatatttaa tattatcgtc
    11581 ttaatcactg catagacagg tatgcaaatc agcatgccta atattcctgc gacacttcct
    11641 gctccaatca ataccaagat gatcgtcaaa ggatggatat tcattgattt cccaaataac
    11701 aacggcttga tcaaatttcc atctaattgt tggatcacta aaatcgacaa cgccatatac
    11761 aaagcttgaa atggagagat aaacaaaccg acaatgattg ctggtaccgc tccaataaaa
    11821 gggccgacat acgggataat attcgtgatt ccgcaaatga ccgcaagcaa caagctataa
    11881 ggctgatcga atattctcat tgccaaaaag ctcattacac caataatcaa agcatctaag
    11941 atcgtactgc tgatataggc agataatgtt tcgtttaatt cttttaccgt ttggcgtaac
    12001 tctttacgaa tcgctttcgg aaagaagtga gacaacgcat caataaattt gtgcccatcc
    12061 ttgaacataa atagcaaaat aaatggaacg gtaaataaca aaataaaaaa ttgcatcata
    12121 aatgaaatga tttttgagac acttgcagat aaactgacta atatgatatt gaacaaatta
    12181 ctaagagata aatttgctga tgctaattgt tcttccaaat tgaaattttt aaactcttcc
    12241 cgctgcgcca attcatttaa ccatttgctt gtttcatctg catacattgg caagctctgg
    12301 gtcaattgaa ttgtctgttc cattaactga ggcactacat tcatgacagt taaaacaact
    12361 aaaatgatta cgaccaaaaa cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt
    12421 tcgagaaaaa caacgattgg atcgaacata taatagataa aaccagcaac aagcaatggt
    12481 aaaaaaactg ccgaaagcat tttcaatatt ggactaaaaa tattcggcat ttgtaaaata
    12541 aagtacacgc cgatgatcgt gagtactagc tccactgtcc agaaaaataa ttttgattgt
    12601 ttaaaacgtg attgcataca tctcctccaa ggtcctttga tacttctatt ctatcattta
    12661 acaaagagtt acgcgctgag attttcattt gcttaaaaaa aacaaaaaaa ataaacatat
    12721 tttaatgagt agacgagtac ttaaacaaaa acacggtttg gagtagtgac aaaaattata
    12781 ctgcagtccc tttttccaaa taaacggtgt tcaaaagctt gcatcttcgt aataagccca
    12841 gccaaacgaa aaatataagg aactattttt atttggctgg gcttattact tgatgtagga
    12901 catctatttt acaacctcag atcatttatt gcgcaatact gactaaatgc aactttgtga
    12961 tatttttgtg tacggacaac tcctcgatca catcggcata agtgagccct tttggaagat
    13021 caatcgtata gatatttttg tacactcggt agtcatcaac gaattttacg tcaaaattca
    13081 catcttcgat tacgatattt ttctcttcaa aataatcggt aagaaaatct ttggtttctt
    13141 ttctatggat aaattggatt tctaactttt tagtcgtagg tacatggatc acgcgtttca
    13201 ctactgtgag tgcaatagca atcccgataa aactgatgat cgcaatggaa tagtaaccca
    13261 tgccgatggc gatccctaat ccagcaattg cccataaaga agcggcagtc gtaagtcccg
    13321 tcaccgattg tttcgtgacg atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca
    13381 cttgagcaat cagccttgct tcatctgaac gaatcgttcc agcaagctgt ggatactcta
    13441 aagcatctct taatgcacca ctagcgatct caacttggat caatgcaatg atacatgctc
    13501 ccatacaaac taaaatatgc gtacgcatac ctgctggacg atttttatat tgtcgttcaa
    13561 aaccgattgc tccgccaaat agcatagcga tccctaaacg taaaataatc tctggaattg
    13621 tcaataccat atcattcatc taaacaccct ccagtccaat atatcatgtc tgaaaggagc
    13681 agacaaaaaa agtgcaattt ttacctctta cgaaaaaagg actgtgacaa aatttttgtc
    13741 atagtccttt ttatgaataa acggtgttca atcagctgac cctcggtatt actcaacaaa
    13801 cggcaaaata tggggagcta tttttccttt gtcttctcta atactcaggt caaaacgctg
    13861 atttcacaac ctctttagcc tactattgaa aattacaact tacttgctgc ttcttcatcg
    13921 atgatcacaa caacattgtc gtgattttgc aatacacttg ctggcaaatg attcgttaca
    13981 ggtccttcaa tcatcccttt gattgcatca gctttgcttt cgccaaatgc catcaagacg
    14041 atttctttac ttttcataat tgaaccgatt cccattgaaa cagcttttgt tggtacatct
    14101 tctgctttat cgaagaatcg tttgtttgca ttgattgttg attctgttaa gtcaactact
    14161 gaagtcgttc cgtcaaatga agcaccaggt tcattgaaac cgatatgtcc attttgcccg
    14221 attcctaaga tttggatatc gattggatgt gcatcgatga tttgatcata ctttttgcat
    14281 gctgcttcca gatcttctgc ttttccatct ggtacaaacg tttctttgaa tggtttttta
    14341 tcaaataatt ggacatccat gaaatgacgg tagctttgtt catctgaacc gcctagtcca
    14401 acgtactcat ctaagtttac agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt
    14461 tctttatata aagtaactgg tgtgcttcct gttgccaatc ctaatacttt tgcacctttg
    14521 ttcatacctt ctttgatgat ttcaaatgct ttttttccgc cttcttcagc gttttttaca
    14581 cgaatgattt ccatagtgat tgctccttta tattttggta tagcccaatt atagcattct
    14641 ttttttcttt ttacaataat tagtatagac caaattgcat ttttttcatt ttcttgacaa
    14701 taaataggaa tccgtgttag gtttcataag atggttcaat caaacaatcc atgttgcatg
    14761 aaaaccaagc aagtgattca ctgtggacgc tttatgtctg cagttttttt atgaaaaagg
    14821 agttattatg agtatactga cattaaacaa cattacccag caatttggcg acaaaatcct
    14881 ctatgaaggt gtcgatctgc aattaaatgc tggcgaacat cttgggctga ttggacaaaa
    14941 cggagccggt aaaagtacat tgattaaaat catcactggt gagatcttac cagatgacgg
    15001 acagatccat tggcaaaaaa atattcacaa aggttacctc gatcaatatg tggaagtcga
    15061 tgaaacatta acaatcgaag aatttctaaa aactgcgtac gcaaaagaat acgaaaagga
    15121 agaaaaaatt gcttttcttt accaaacata tagcgagacc ttcgatgatt ccttgctgga
    15181 aaaagctggg aaactccaaa cggaattaga ccaaggagtg ttttatcaaa tcgacacctt
    15241 agttgctgag atgagcagcg ggttggggat cgatgtctta ggtttagatt cactgttgaa
    15301 aaatctaagt ggtggacaac gttcaaaggt gattttagca aaactacttt tagaaaaccc
    15361 agatgttctg atcttagatg aaccaaccaa tcacttagat gatcagcata tccaatggct
    15421 gacgcagttt cttcaggatt ttgacggtgc gtatatcgtg atttcccatg accaagaatt
    15481 tcttgatcag atcaccaccc atatcgcaga catcgagttt ggaaagatta ctaagtatac
    15541 tggccatcta aaacaagcat tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca
    15601 atatcatgct caacaaaaac aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc
    15661 cggttcccga tcaacgatgg caaaaagccg acaaaaacaa ttagataaaa tcgaacggct
    15721 gacgcctcca gcttcagcaa gtaaagctgt ctttgatttt ccattctcgc ctattgtcac
    15781 gacattagca gtggaaacaa ctgatttagt catcggatat gatcgaccgc tacttgcacc
    15841 catcaatttg accatccgct ttggtgaaaa tattgccatt cgaggcttca acgggattgg
    15901 aaaatctact ttattaaaaa cactgatcgg tgagattgaa aaaataagtg gcgatttcca
    15961 cttcccagat aacacaaaaa tcaattactt ttcacaagat ctgtactggg aaaatccatt
    16021 agaaacacca ctacaatatt taagcagtca atttccaaaa gcgactatca aagaattacg
    16081 tcgccaacta gctaaagcag ggttagttaa tcaattagca agtgaacctc tcactacatt
    16141 aagtggtggg gaacaaacta aggtgaaact tgcacagttg acaatgaacc aaggtaattt
    16201 actgattctc gatgaaccga cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga
    16261 cgggatacag aaatttccag gaacagtaat catcgtctct catgaacaag aattttatca
    16321 agatcttgtg caacgagtca ttgagattga accaaaataa acgtttgatt gaatagttga
    16381 aaaaaaggag ctgtgacaaa agttttgtca caactccttc ttctgaataa atggtggcca
    16441 attagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct atttttattt
    16501 ggctattctt attacttgat gcagaacagc ttttcacaac ctcttttcgt ctatttttta
    16561 ttttcgagat ttctctttcc tttaaaaaca actgtttgaa agaagaacgc acatccttga
    16621 aaaacaagcc cgctaaccgt tacacctaac cagccatact gctgccacat tgtcgtccct
    16681 agcaatgaac cagccgcacc tccaataaag tacataaaca taaatacagt attatttcgg
    16741 ttgcttgctg cttcacctag cccttgtact cgtgtttgat tggcgacttg accaaattga
    16801 ttcccgacgt ctaataaaat aatagctaaa agcaaaatgg ccaaatggct tccgccaacc
    16861 aataaagcaa taaagcttat tgtttgcata acaagtccca tatacacgat ttttctttca
    16921 gaataagaat ctgccagtcg tcccacgata ggtgcagcaa ctgctccaga caaaccaaaa
    16981 atcgctaata tccctgcttc gaatgttccc caattgtagg cgggactgct gatataaaaa
    17041 atcaacgtag accaaaagat cgaaaatgtt ccaaacatga aaaatccact gactgccgct
    17101 tctcttaata gacgttgaga agcaactaaa tgcggcaaac ttttaagtga atcaaggtat
    17161 gaaacagctg tactactttg ctggacagaa cgcggcattt tccagtaaag cagaagtgta
    17221 agaattccga ctgcacataa agcaaataaa tagatcatcc gccaagaaaa aagactggca
    17281 acaataccag ataccgtccg agaaagcaaa atccccacga gtaaaccgct aagcaatgta
    17341 cccatgacct tccctcttgc ttttggtccc gctagtaccg caccgtaagg aatgatgatt
    17401 tgcggcacga tcgaaagtaa tcctattaaa aaagaagcgc atgcaaataa cgtaaacgaa
    17461 ggcgcaaaaa aagcggccaa cagagagacg gcagataacg ctgccattcg aatgatcaat
    17521 tttggacgat ccaccacgtc ccctaaagga actaaaaaca gtaaccctaa cgcatagcct
    17581 aattgggtca acatcgtcaa tatcccgact gcacttgtgc taacagataa actagctgct
    17641 acttttgttc caatcggctg gatgtaatac atattggcaa caacaacgcc acaagtgatt
    17701 gataatagta aaataagcga agttgttaaa ggtttttttt cattactctt cattgatttc
    17761 ccccatttcc tatcttagtt tacaacttat ggaaaagatt ggaaatagaa aaatacaact
    17821 attcgccaca cttattattt gtgcttttgt tttccttgtt ttttcttgcg atctttataa
    17881 atgaagacaa ctaaccctac tagcacagta acacttgcaa ccaacgtgac tgcagttttg
    17941 ttatatccgt gaaacagagc ttctccacca cacgcataca acacagacga agggatgact
    18001 ccaatcacga tcgacaatat gatttgctgt agaggcaatt tcatcgcatc tgcagcaaaa
    18061 ttgaccatag aagatggaat aaccggtatc atatagccta acattacacc tatttttgga
    18121 tgtttcattt gtcggattgc ttgcacccaa tgattcgttt cggttttctt atctaaaaat
    18181 tttaagcgat gcataaggaa aattgcagtg atgttaccaa gagcattacc caaaatgttg
    18241 atgatcgcac ctgtcagtgg tccaaagctc aaaccagcaa tgactccaac tactgaagta
    18301 ggaatacctg gaacagcaca catcaccgct gtcaatacga tcaagagtat cgatgcaaaa
    18361 gtcccatgcg aacgaatatg tgcgagtagc tctgccttac tcgctctcgg atttaaaaat
    18421 aattggatgt ctgtctgata ttctaaatac aaccgatagc ccactaccag tatcagaatg
    18481 attcccaacg agatcaatat caatcggatt attttctttt tcatatttcc tcctgaaaaa
    18541 aatctatatt cattaaataa tagtttaaca taaaaacaaa aaagcaccga aatccaacat
    18601 aggatctcag tgcttttaaa tagtaaaaat tatttaacag ttacagatgc gccaacttct
    18661 tcaagtttag ctttcaattc ttcagcttct tctttagata cgccttcttt aactggtgca
    18721 ggagcgccat caactacagc tttagcttct ttcaagccta aaccagttgc ttcacgaact
    18781 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    18841 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    18901 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    18961 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaattg
    19021 ttctttttta tttaatgctt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    19081 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    19141 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    19201 gactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    19261 gtccatgatt ttcgctggtg cgactacatc ttcgttactg aatgcaacag ctgtaggtcc
    19321 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaataga
    19381 atttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    19441 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    19501 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    19561 gtttcacctc ctgaattttg atggaagagt tttttgcact gaaaaggcaa gtcactttta
    19621 tagttacttt gctcaaccag tattattcat cacctattca cttcgttcat agtgattcta
    19681 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatctc
    19741 ttcacttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    19801 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttgatg ccacctactg
    19861 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    19921 agaattattt ttcagttgga tcaatcaaca ttctaatatt taattagaaa gaagcttggt
    19981 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    20041 tagctgctga tggtttagct tttagcaata catcatggat tgctgcaaag ttttcaacta
    20101 atttttcatc gtcaaatgaa acttttccga ttggtacatg gatatttcca gctttgtcca
    20161 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttacg tccattgtaa
    20221 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatactcgt cctagacgac
    20281 ctacagtagc catcatatca ggtgttgcaa caactacatc aaagtcaaac catccgtttt
    20341 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    20401 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    20461 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    20521 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    20581 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    20641 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    20701 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggtg tatagattat
    20761 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    20821 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    20881 actttcgcaa ctttattttt gtttggttcg cctgaacctt tttcgatttt agctgctttt
    20941 ttcaatagaa cagcagctgg cggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    21001 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    21061 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    21121 ggagttgctt tacctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    21181 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    21241 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    21301 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    21361 taaacgctaa atttattcta aaaaaacaac aatttttcgt tctcatttct tcttttatgt
    21421 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    21481 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    21541 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctct cacactgccg
    21601 gagctgctag aatcgggaaa atcatacgta gtatatttgg cgaacagcct gattctgtag
    21661 atatttatct atatgaatca tttgcaaaaa cctatcgggg gcacggaaca gatattgcat
    21721 tagttggcgg cctgttaggg atggaacccg atgatgaacg tttagctgat tctttgaaat
    21781 tggcatatga agccggtatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    21841 caaactctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtc actggcattt
    21901 cgatcggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    21961 tgggtactcc taccttgatc gttgtccacc aagatgttcc aggtatgatt gctaaagtga
    22021 ctagtatact ttctgagacc aatacgaata tcggcactat gacagtgaca cgggaatcaa
    22081 aaggtgaaaa tgccattatg ataattgaag tagatgatcc tcaagtagaa gaaacggtaa
    22141 aaagactcaa acagctgcca aatatcgaca gcgtaaatta tttcgattaa ggagctaaaa
    22201 aaatgttttt atcgattgaa gaattagtag atcaagcaaa agcttatcca agtgtagcag
    22261 aattgatgat tgcagttgaa atggaaatga gtggccgttc ccgtagtcac atcatcgaga
    22321 tcatggaaaa aaacctctca gtaatggaac aatcaatcaa cgagggaaaa gaaggtgtta
    22381 cctccgtcac aggaattacc ggtggtgatg caaaaaaatt agatgattat ttaaatgagg
    22441 cagacttttt aagtggagaa acaatcttaa aagctgtacg caatgctgta gctgttaatg
    22501 aagtcaatgc aaaaatgggg ttgatttgtg ctacaccgac tgccggaagc gcgggtgttg
    22561 ttcctggtgt gctgatggct gtccgagatc ggcttgattt gacacatgaa cagcaattgg
    22621 aattcttatt taccgctggc gctttcgggt tagtcatcgc aaacaacgct tctatcagtg
    22681 gtgcagaagg gggatgtcaa gcagaagttg gttctgcaag tgcaatggca gcagcagctt
    22741 tggtttgtgc aaaaaatggg actcctcatc aagcagcaca agcagtagcc attacattga
    22801 aaaatatgat gggactgatt tgcgatcccg ttgcaggctt agttgaagtc ccgtgtgtca
    22861 aacgtaatgc tttaggctct tctcaagctt ttatctctgc tgatatggca ttagcgggta
    22921 tcgaaagtgt cattccgccg gatgaagtcg ttgctgcaat gtaccaagtc ggacgtcaaa
    22981 tgccgtcgat tttcaaagaa acagcagaag gtggcttagc tgttacgcca actgctaaac
    23041 gacttgcgga agagatttta aataagaggg cgtgataaag ctccttaatt tcgagtatta
    23101 agagattagt tatcttcata acaaaaaacg aagagaggac taattgccct tctcttcgtt
    23161 ttttattaat ccatcaattt cgtatttttt acttcatcga tcgtttttgt tcctgcaagt
    23221 tgcatcgtga tcgacaattc tttgttcagg tgttcaaaaa cagatgttac tccttctgcc
    23281 ccacctagat tcaacccata aatcactggt cggccaatcg caactaagtc agcaccactt
    23341 gccagagctt tgaagacatg ctctcctcga cggacaccag agtcgaaaat cactggtaca
    23401 cgtttgttga ctacttcggc aatttttggc aatacttcaa aggacgcagg acctccatct
    23461 agttgacgtc caccatgatt ggatacccag ataccatctg cccctgcaga gatagcgatt
    23521 actgcatctt ctggcgattg tattcctttt acgataactg gaagatgggt gatttctttg
    23581 attgttttga tgtcatctgg tgtcaagcct tgttttgcag ctgcataaat ttctgcgatt
    23641 ccttttcctt cgccattacc acttgcactt tgttcgctat acgcagctaa gttaggcatt
    23701 ggtaatggaa attggaattg attgatgacg tcttcttcac ggtaaccacc gagcgttgaa
    23761 tctgccgtta aaatgatagc tttagcccct gcttctactg ctttgttcaa aatgaactca
    23821 ttgaatctgt cgtctttact catatataat tggaaaaact gcggtgcgcc tggcgctgca
    23881 tcggctgcgt ctttgattgt cgtatttgca taagtgctga tggaaaagat tgatccagca
    23941 gcagctactc ctttagctgt atcagcttcc cctttttcat gtgccaatcc ttgggccgct
    24001 gatggcgctt ggataatcgg tgtatctaat tcaattccga aaacagatgt atgcaaatcg
    24061 gctgagtcga ttccgcgtaa tatccgtggc atgattttct ttgtattaaa agaagtcgta
    24121 ttttctttca tcgtccactc atcttcagaa ccaccgcgaa tatagccaaa agctcctgct
    24181 tccatgcgct cttttacacg accttctagt taggcaatgt ttacaatatc gattggatgt
    24241 tcttcattac ttgtatgata agtcatagta gatccttctt tcatcttctt gattacatat
    24301 aaatcatact aaacttacaa aaagtaagca aacatttact ctcagatact tttatcgcta
    24361 ttctttccaa ataaatcgac ttacaagctg atctacttct tggttttcat gaagcatact
    24421 atgagaggca ttttcacctg tcactacttt ttcctctatt tcatttccgc gttgcttcaa
    24481 caacgcatag accgctaatg cactgtttaa cggtactgtt ccatcactca agtctgtttc
    24541 atctagttga ccagcaatca agaaaaatcg agtggttata ggaatattac cgatcatttg
    24601 ctggtaatct tgatagcggt tagaaacgac agcaggcccc tttgctaact catctgtcag
    24661 agactgcgca ctatcatcga caaaatcatt aaaaggcgcg ccaattgctg ttaatgtctg
    24721 aacttgtggc aatgacgtat cctgtccata cgttcctaaa tagcgtagtg cactaactcc
    24781 tcccatggaa tgccccacta tgttgacttt atctatgccg tagttttctt tcagatacaa
    24841 aagacaagtt ttgatccatt ctgcttgatt ccactcgtta tttttattat cttcaaacaa
    24901 tacttggacc atcggattgt ctgcttttcc agacaaatct cctgttgctt gtatttgtcc
    24961 atctgttcct actgttaaaa ccaattcttt tactgcttgc tgattttttt ccaaacgccg
    25021 caacatccct ttaaaggaat tttctgtccc gctgtagccg tggaaaaaaa gtgttggaat
    25081 agtggttttt tctatcactt tactggattc tgccgttttt tctttatcag aaacttcagt
    25141 agtatttatc ttcgtcgtct ggcaaccact taacgcaaac aaactgataa ggactaaagc
    25201 tgctgcagtt gcacgtttgc ataaattttt gattggttct gtcatacacc tcttcctttc
    25261 catatagaaa aaagaaagac ttgaaaggcc aatcatatga tcgacatttc aagtctcttt
    25321 attaatcgat tttatctact tgatcgaaat caagttctgt actggtttca cgtccaaaca
    25381 tatcgatgtt gactttcaat ttttcttttt ctccgtcaac ttctgttact tgtccttcta
    25441 atccagcaaa tgcgccttcg atgattttca cgacttcgcc aacttctact tctagatcat
    25501 tttgtcgcag gctcataccg atagaacgca agatatgatt gacttcttct ggaagtaatg
    25561 gtgctggttt gcttccggct ccgtgtgaac caacgaatcc tgtcacacca ggtgtgttgc
    25621 ggacaacata ccatgaagcg tctgtcatga tcatttcaac aagtacgtat cctgggaatg
    25681 ttttatgaac gatttctttt tctttcccat ttttgacttc agtttctttt tcttcaggaa
    25741 caacgacacg gaaaataaag tcggccattt tcatactttg cgcacgagat tcgatatttg
    25801 cttttacttt gttttcatat cctgaatatg tatgcaagac ataccagttt ttttcaaaag
    25861 attccattga aggctcctca tttcattgta aaataaaaaa acctcagtac acccgaagtt
    25921 tttctttcgc ttcattatag cactaaaagt acgttgtggc tagcgagtta ttttaaaatc
    25981 cagccaaata agctttggat ggctgtatcc attacgtaaa acatcaatcc gaaaataata
    26041 gaagtttcga tgactactaa cgtatcttta cggagttgtt ttctgcttgg ccaagttact
    26101 tgtttgattt catcttttac gctttttaga aatttcatga gacaacctcc cttgctactg
    26161 tttatttcgt ttctttatgt aatgtatatt gattacaata tttacaaaac ttattgattt
    26221 ccaaacgttc gccgcttttt ccttcactga ctgattttgt gtagttgcga gacccacaaa
    26281 cggaacaagc gagtgctgct tttttctttg ccatttactt ctgccccctt gctgagaatc
    26341 agacttatac ttcataacag tatcacgtaa ctttttactt gtcaatttgt gaaataaact
    26401 atctattgtt ttggataaaa ttatgatatg attaatttga aattaagcta aaatgagaca
    26461 attggaggta tagcgatgct attaaaaaca cttgtatata agaaaaaaga tctgacaacc
    26521 gttacagaga ctagtacatt agaagaagca ttaaaaattt tagaaacttc cggataccgt
    26581 tgtgtgccga tcttagattc ttcaggtaat attttccgtg gaaacattta caaaatgcat
    26641 atctatcgtc ataaagcaaa tggcggtgat atgacgcttc ctgtcactca tcttttgaaa
    26701 aacgctacaa agttcattta cctggatact tctttcttca aagtattctt tacgatcaaa
    26761 gagcttcctt atattgctgt ccttgacagt gaaaatcatt tttatggtat tttgacacat
    26821 agtacgttgc ttaatatgct ctctcaatct tggagcgtag aacaaggaag ctatgtactg
    26881 acaattgctt ccacaggaaa gcaaggagat ttggctgcta ttgcaaaaat catttcaaaa
    26941 cacagcagca ttgccagctg tattactctt gatatcgaaa aagacgaata cattcgacgt
    27001 acattgatca cgctaccggc taacactgaa gcgaaagtat gtgaaacgat cgtcaaccac
    27061 ttagagaaaa agaattataa agtggtagaa attgaaaatt tgcaaaacca agaagcataa
    27121 tcaaaaagcg aaaaaagaaa acagctaatc tgctttcttt tttcgctttt tataattaga
    27181 cacgcttttc aaaatcaagc gcgcgatcta aataatacat caatggtgct gaaacagcca
    27241 tgacgataaa ctcacttaat gcaagcgtgc cataggttgt ccaaaatgct tggccacctg
    27301 ttggaactaa catataagca atcagtccca tgctgatggt aaagaagatc gtgttcaata
    27361 ctaaacgcat ctttacattt ttcactttgt tttgcaataa agctgtcagg cctaaagaaa
    27421 gcaatgtttg gccgccacca aaaaccacat ccatgatacc aaaaccaaag aaggcattat
    27481 agacgatgac cccgcctaaa actccccaaa gcaatttacg gttaaagaca actaaatgat
    27541 tcaaactttc tgaaatacga aactggatcg gccctgagga tacaggcgca actaaaatag
    27601 ttaacgctag ataaagagcc atgatgatgc catttacaac gattacggat aaccgttgtc
    27661 tgcttttcgt tttttgcatt ttctctcctc ttttctccta gtttttttaa cgcgggatgg
    27721 ttgatgaacc gcggttatgt gaaacaaaca ataattataa cagacttttc atttatttca
    27781 atccgtctta tctgctttta aaaaagcaaa agcttaaaca atgtaaacgt ttgctaaaaa
    27841 tcgtgggata tggtataaag taagtgtaag gggtcataag aattcccagt aaagcagacg
    27901 aagtaggatc tgcaagaaca gaattgttgt gaaacttaaa taaataaaaa aggatgtggg
    27961 aatttgagca acctcttact agagaacggt ctagcaagca gctgaagatc taagtggaca
    28021 tgccgaacct tggaacttct ccttacgtaa gtaatggatc atgcgcttgc taggcttttt
    28081 tgtgtttttc ggtcttcgga cctttttcat tttcctagac ttctgctata ataaccaaga
    28141 attaattttg taacagattt ttttgaaaaa acaagtttct ttcattcaaa aaatagaaaa
    28201 agcttgctaa ttattggtta accagcaaac aggagtatta aaatatcttg ttttattgaa
    28261 tagcaggagg aaatcatgaa attccattat catatattga tcaatccatc ggcaggcagc
    28321 ggaaatggct ataaagtagc agaaagaatt ttaccggtat taaaaaataa gcatattgac
    28381 tatacacttc attatagtga atacaaagga cacgatgctg agattgctga aactttggca
    28441 aaagaaacat tgattccttg gatagaagaa gaacagaaaa caatcgatac tttccccctt
    28501 ttgatcatcg taggaggtga cggcacctta catcaggtac ttgatacttt ctatcagatg
    28561 gaagtagaat tcccagttgc ttatattcca gcaggttctg gagatgattt tgctcgaggg
    28621 gcaggccttc caaaaaatcc caaaaaagga cttcagctga ttttagcagc tcaatcacca
    28681 gaaaaagtcc acataatggc ttatgaagaa aaaataagtg aaaaaaaagg aatagctgtt
    28741 aataatttcg gtataggatt agatgcagct atcgtccatg cgacaaatca ttcttccaca
    28801 aaaaagcgat tgaacaaata caacttagga tcattttcgt atttattctc tattctccgt
    28861 gctttattca ctcaaaaagg atttccaatt ctggtagatg caggaggaaa acaattaagt
    28921 ttttcaaacg catttttatg tacagcaacg aagcatcctt tttttggcgg aggaatcgcg
    28981 attgttccaa ctgctgatgc gtctaaacca gtcattgatt ttgtcgtggt cgaacgaatc
    29041 aatttcttta aaatcttgtg gctcattctt ttacttatac agaaaaaaca attgaaatcg
    29101 aaatatttcc atcactacac aacaaacaga ttacgtatcg tttcaacaac accgcaacat
    29161 ggtcaagaag atggagaaga aatggaaaaa cgaccttttg atatcacaat aaccacaact
    29221 aaacagttgt tttggtgtta ataaaagagg ttttgtcaga agctacatct ggaacaccgt
    29281 ttattcggaa aaaaggactg tgacacgact tttgtcacag tccttttttt actatgcagt
    29341 taattctttg cccaattggg ttgccatcga tagtttcttt ctttacccaa aataaattgg
    29401 atcattgaaa acaccgcagc aaaagcaact aagacacaac cattgaacca aaaagattct
    29461 gcaaatgttt gattgtaact gagtgtttca ctgatcaagt tattattttg aacaaaccaa
    29521 tatgttggtg tgaatgccgc tattttgcta accatttctg gcaagtattc tgctggtaca
    29581 aacaccccac cgataaagca actgcctgtg ataaaaatat ctttgatccc accgctggtt
    29641 tctgagtttt ttaccaatga agtgatacaa gccgaaaagc taatgatcgg caataaaaac
    29701 aagaaagtat ttaaaatatg ataccatacc gttatatccc acgaggtgtg gatcactaac
    29761 agaaccatta gaagtaagaa gccactgaac aacagcagcg agtaactaat caaactaaag
    29821 gttactttgc gattcaagtt tcgtttggta atgggcgcac tctggtttcg tttactgatc
    29881 tctttgcgat taaatgctag attgatgacg ctgaaaccgc taaaaatgga tagaaacaac
    29941 ccataagaca agaaattaaa ggttgtgccc gtcaacagct ttttcatccg ctgactatat
    30001 cccgcactta gctcaacttc tccagtaacc gagagtgcca tttgtgtctg ttccatagct
    30061 gcatcgattg ttttatcttc ttcttgaaac agaatcagtg tttgcaaaaa ggtgttcact
    30121 tgctgtgtaa caactgtttt agaaaaggca tctgggcgac tctgtatctt gatcgtcggc
    30181 atctttccta cttcgacttg ttcggtaaag tctgcaggca gatgtaagat aatgtttaca
    30241 cgattaaaat aaagagcatc atcgatttct ttttgtgagg ttccatccaa tgtaacgact
    30301 gtttccttgt cattcaaata tttacgaagg ccatcgacta caggcgactc ttcttcacta
    30361 atcaaggcga ttttcgcatc ttgcagagag gtattttcaa catcaatatt ttgcgcaaaa
    30421 aagaatgtgg caacaacagt gataattaca cctatcagta aattcgattt atttgcttta
    30481 agcaatgtcc aaatcgtttt ataaagcgtc ataacgcacc cctctttcca tccaagcatt
    30541 tcctaacgca aaaaggaaaa taaatccgat caaccataga agattggtat aaaaactagc
    30601 caaatcagtg tagaaataaa gttt
//
LOCUS       DACDSR010000024        29618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDSR010000024 DACDSR010000000
VERSION     DACDSR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.024"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            407..1516
                     /gene="pdhA"
                     /locus_tag="IWK15_001140"
     CDS             407..1516
                     /gene="pdhA"
                     /locus_tag="IWK15_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9546757.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1519..2496
                     /locus_tag="IWK15_001141"
     CDS             1519..2496
                     /locus_tag="IWK15_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9546758.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2521..4164
                     /locus_tag="IWK15_001142"
     CDS             2521..4164
                     /locus_tag="IWK15_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9546759.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            4172..5578
                     /gene="lpdA"
                     /locus_tag="IWK15_001143"
     CDS             4172..5578
                     /gene="lpdA"
                     /locus_tag="IWK15_001143"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9546760.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5740..6015
                     /locus_tag="IWK15_001144"
     CDS             5740..6015
                     /locus_tag="IWK15_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9546761.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            6021..6788
                     /locus_tag="IWK15_001145"
     CDS             6021..6788
                     /locus_tag="IWK15_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9546762.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6956..8785
                     /gene="typA"
                     /locus_tag="IWK15_001146"
     CDS             6956..8785
                     /gene="typA"
                     /locus_tag="IWK15_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9546763.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            9046..10818
                     /gene="recQ"
                     /locus_tag="IWK15_001147"
     CDS             9046..10818
                     /gene="recQ"
                     /locus_tag="IWK15_001147"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9546764.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10932..11234
                     /locus_tag="IWK15_001148"
     CDS             10932..11234
                     /locus_tag="IWK15_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9546765.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            11237..12400
                     /locus_tag="IWK15_001149"
     CDS             11237..12400
                     /locus_tag="IWK15_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9546766.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12426..15854
                     /locus_tag="IWK15_001150"
     CDS             12426..15854
                     /locus_tag="IWK15_001150"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9546767.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15875..16999
                     /locus_tag="IWK15_001151"
     CDS             15875..16999
                     /locus_tag="IWK15_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546768.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16989..17426
                     /locus_tag="IWK15_001152"
     CDS             16989..17426
                     /locus_tag="IWK15_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9546769.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17410..17748
                     /locus_tag="IWK15_001153"
     CDS             17410..17748
                     /locus_tag="IWK15_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9546770.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17867..18427
                     /gene="rsmD"
                     /locus_tag="IWK15_001154"
     CDS             17867..18427
                     /gene="rsmD"
                     /locus_tag="IWK15_001154"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9546771.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18424..18915
                     /gene="coaD"
                     /locus_tag="IWK15_001155"
     CDS             18424..18915
                     /gene="coaD"
                     /locus_tag="IWK15_001155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9546772.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18905..19948
                     /locus_tag="IWK15_001156"
     CDS             18905..19948
                     /locus_tag="IWK15_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9546773.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            20107..20835
                     /locus_tag="IWK15_001157"
     CDS             20107..20835
                     /locus_tag="IWK15_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9546774.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20951..21196
                     /locus_tag="IWK15_001158"
     CDS             20951..21196
                     /locus_tag="IWK15_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9546775.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            21193..21411
                     /locus_tag="IWK15_001159"
     CDS             21193..21411
                     /locus_tag="IWK15_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9546776.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21547..22086
                     /locus_tag="IWK15_001160"
     CDS             21547..22086
                     /locus_tag="IWK15_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9546777.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            22079..24352
                     /locus_tag="IWK15_001161"
     CDS             22079..24352
                     /locus_tag="IWK15_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9546778.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24390..25436
                     /gene="holA"
                     /locus_tag="IWK15_001162"
     CDS             24390..25436
                     /gene="holA"
                     /locus_tag="IWK15_001162"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9546779.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25525..25779)
                     /locus_tag="IWK15_001163"
     CDS             complement(25525..25779)
                     /locus_tag="IWK15_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9546780.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(26179..26448)
                     /locus_tag="IWK15_001164"
     CDS             complement(26179..26448)
                     /locus_tag="IWK15_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546781.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26918..27373)
                     /locus_tag="IWK15_001165"
     CDS             complement(26918..27373)
                     /locus_tag="IWK15_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9546782.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27511..29439)
                     /locus_tag="IWK15_001166"
     CDS             complement(27511..29439)
                     /locus_tag="IWK15_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9546783.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         9879 a         5165 c         6264 g         8310 t
ORIGIN      
        1 tgtttctttt ttttcggaaa aagaatacga tataaagaaa ctaagaaaaa accattattt
       61 gtacgtccgt tcacgatgtg taaataaaat aaatgtttgc aacaaaactg tataacacca
      121 acgtttttaa atagaaacag atggttgtaa gtttgattag aaaatagaaa aaatgcagat
      181 gaaaaataaa acgctttcct tattgtgatt ttattcacta atgctgttaa tatgcggatt
      241 ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct cataaatcca
      301 tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact gtttcaatcg
      361 cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg caaacaaaaa
      421 agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc cagtatatca
      481 agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt tatccgatga
      541 tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc aacgttcaac
      601 agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac aagaggcaag
      661 tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag gttatcggga
      721 tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat ggtctcgagg
      781 acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac aaattatcat
      841 cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac gaaacaagaa
      901 aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt tttacgaagc
      961 aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa acaacgggta
     1021 tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac aaaaagctgt
     1081 cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag tatatactgt
     1141 atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa tcgaaacact
     1201 gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct accgtacaaa
     1261 agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta aatatttgac
     1321 ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa ctaaagaaga
     1381 aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt cagatttctt
     1441 gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa tttatgaagc
     1501 gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac agatgcctta
     1561 gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt tgggaaaaac
     1621 ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga ccgagtattc
     1681 gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc gttagaagga
     1741 tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat ggacgaagta
     1801 gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct acccatcaca
     1861 attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga taatttagaa
     1921 ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc ttatgatgca
     1981 aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct agaacacatg
     2041 aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt tcctttggat
     2101 aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg agcaatggtg
     2161 cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt tgaaatcatt
     2221 gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt ggaaaaaact
     2281 ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc gcaagtagtt
     2341 tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg agtttctgca
     2401 cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa tgcaaaagat
     2461 atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg aaacaaagtc
     2521 atggcttatc aatttaaatt gccggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
     2581 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
     2641 aatgacaaat cagtagaaga aatcccgtca cctgttacag gaacggttaa aaatgtgatt
     2701 gttccagaag gtaccgtagc aaatgttggg gatgtattag tagaaatcga tgcaccagga
     2761 cacgaagaca atgaagggga cagtggtgta gccgcagaat cccaaactcc agctaaaccg
     2821 gctgctgaac ccactgttga tacagaatca gctggatcgt caagcgaagg agtcttccaa
     2881 tttaagttac cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc
     2941 aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca
     3001 gtagaagaaa ttccgtcacc tgtcacaggg acagttaaaa acgtgattgt tccagaaggc
     3061 actgtagcaa atgttgggga tgtattagta gaaatcgatg ctcctggaca caatagtgca
     3121 ccgtcaacta gtgcgccatc agctgaagca ccaaaagaaa aagtggaaac atcaggttca
     3181 gcaagtgtcg ttgaagctgc cgatccaaat aaacgtgtat tggctatgcc atctgttcgt
     3241 caatttgccc gtgaaaaaga tgtagatatc tcacaagtaa ctgcaactgg aaaaggtggc
     3301 agagtaacaa aagaggacat cgaaaacttc ttggcaggtg gtccaagcag tgcaccagcg
     3361 aaatcagaag cgccagaagc ggcagcaccg aaagaagcag ctcctgcagc agaaagcaaa
     3421 ccagctgcac cagcaaaacc attcaagtcc aacttaggag acttggaaga acgtgtagca
     3481 atgacgccaa cacgtaaagc tatcgctaaa gcaatggtca acagtaagca tacagctcca
     3541 cacgtgacat tgcatgatga agtagaagtg tctaaactat gggataatcg gaaacgattt
     3601 aaagaagttg ctgctgcaaa tggtacgaaa cttactttct tgccatatgt cgtgaaagct
     3661 ttaacagcga ctgttaagaa atacccagta ttgaatgcat ctatcgacga tgcaaaccaa
     3721 gaaatcgttt ataaacatta ctacaatatc ggtattgcaa ctgatacgga tcacggacta
     3781 tatgtaccaa atgtgaaaga tgcagaccgc aaaggaatgt ttgctattgc agacgaaatc
     3841 aatgagaaag caaaattagc tcatgacggc aaactatctg cagaagatat gcgcaatgga
     3901 acaattacga tcagcaatat cggatctgta ggtggcggtt ggtttacacc agtcatcaac
     3961 tatccagaag tagctatttt aggtgtagga acgattgcac aacaaccaat cgtaaatgct
     4021 gaaggcgaaa tcgttgtcgg tcgtgtgatg aaactgtcat taagtttcga tcaccgtatc
     4081 gtagatggag caactgcgca acaagcaatg aataatatca aacgtctttt agctgatcca
     4141 gagctattaa tgatggaagg atgataaaca aatggtagtt ggagattttg ctgtagaatt
     4201 agatacagtt gtaattggtg cgggacctgg tggctatgta gctgccatcc gcgctgctga
     4261 aatgggacaa aaagtagcaa tcattgaaag agagtatatt ggcggtgttt gtttgaatgt
     4321 cggctgtatt ccttcaaaag cactgatagc tgcaggacac cattatcaag agtcactaga
     4381 ttctgaaatg tttggagtaa ctagtgaaaa cgtcaaacta gattttgcaa aaacacaaga
     4441 atggaaagaa aacaaagttg tccatacgtt gacttctggt gttggtttcc tattaaaaaa
     4501 acataaggtt gaaacaatcg aaggagaagc gttcttcgta gatgatcaca cactacgcgt
     4561 cattcatcca gattcagctc aaacttattc gttcaataac gcaatcatcg caacaggttc
     4621 ccgtccaatc gagattccag gatttaaatt cggaggacgt gtgttagatt ctactggcgg
     4681 attagcattg aaagaagtac ctaagaaatt tgtgatcatc ggtggcggtg tgattggtgc
     4741 cgaattgggc ggagcgtacg caaacttagg agcagaagta acgattcttg aaggctcacc
     4801 acaaatttta ccaacttacg aaaaagatct agtaaaatta gtagaagatg atttcaagaa
     4861 aaaaggcgta acagtcgtaa cgaatgcaat ggcgaaagaa gcagtcgata acggtgacag
     4921 cgtgacagtg aaatatgcag tggacgggaa agaagaatcc gttactgctg attatgtgat
     4981 ggtcactgta ggtcgtcgtc cgaatacaga tgatatgggc ttagaacaag cgggtgtcga
     5041 agtaggagaa cgaggattga tcacggtgga taaacaagga cgtacgaacg tacctaacat
     5101 ctatgccatc ggtgatatcg ttccaggggc ggcattagca cacaaagcca gttatgaagc
     5161 aaaaatcgct gctgaagcaa tctctggcaa aaaagtagct gtagattata aagcaatgcc
     5221 ggcagtagcc ttcactgatc ctgaattggc ttctgtaggt atgacgatca aagaagcaaa
     5281 agatgctggt atcgaagcaa aagcatataa attcccattt tcagggaacg gtcgggcatt
     5341 atcgcttggt aaaacagaag gattcatccg tctagttacg acgatcgaag ataacgtgct
     5401 tattggcgcg caaatcggtg gagtcggcgc aagtgatatg gtctctgaat tagctttagc
     5461 aatcgaatca ggaatgaatg ctgaagatat cgccttgacg attcatcctc atccatctct
     5521 tggcgaaatc gtcatggatg cttcggaatt agcattaggt ttaccaatcc atatttgatt
     5581 tgaataatac aggcgaaacg caactgaaaa atcagttgcg ttttgttttt tagaattatc
     5641 aataatatta ttatgtaaac ttaaaaatat gacaacttct tggtttatta ccacttccta
     5701 tgctacactg aagaggaata tgaaatagga gagttgaaga tgcaagaata tcagtatcca
     5761 cttgatttag attggacaac agacgaaatg gtcattgtaa tgaagatgtg ggaagatctg
     5821 gaacgtgcga atgaaaaagg gataaacaat gaagcttttt tgaagactta tcagcagttt
     5881 aaaactgttg tcaaatccat tggtgaggaa aaacgattgg gacgggaatt tgaaaaagct
     5941 tccggttatt ctttatatcg tacagtgcag caagcaaaaa aaaatccaaa caaaactttg
     6001 agaatgaacg ggtgagataa atggaacaga tgattgatca tatcaagtcc tggcttgaag
     6061 aagctggtga gaaaatcaaa tcttctcaac aaacattaac aatctcgcaa aaatcaaatc
     6121 gaacggatct agtaacaaat gtcgatcgaa acatccagaa ttttctaatc gaaaaaatcc
     6181 attcctttga tccagaggcg aaaatattgg cagaagaaga agggtgtaat caattatcgg
     6241 atctcgatgg aagagtcttt atcatcgatc ctattgatgg caccttgaat tttgtgattg
     6301 aaggggaaaa tttttgtatt atgctcgctg tttatgaatc cggcgttgga aagcttggtt
     6361 tcatttatga cgtgatgaga aatgaacttt tttggggtgg aaaaggaata ggcgtgtata
     6421 aaaatgatga gccattgcct agaccagcaa ataaatcctt atcggaaggc ttacttggtg
     6481 tgaatagtta tcttttcggt cataataaat atggaatccg agaaattgcg gaaagcagca
     6541 tggggacacg tatgtacgga tgcgctggtc ttgaactagt ggcaatcttg aaaggaaacc
     6601 acattgctta tatttcaaac cttagccctt gggattatgc agcgggaaat gtcttattgg
     6661 aagagtttgg tatggtaaca agcggtcttt ttggtactcc tttgacattt gaaaaaaggg
     6721 aatactattt agcagggact tcttgtgcat atgcagaaat cgcagacctt ttgaaaaaag
     6781 aaggctgaaa aaaaggtaga aatcattcac aaaaatgata aaaacgagca aaagattaat
     6841 tttctcgaaa aaatgaaaac attttactat atttcatgaa aaaagtctgc tataattagc
     6901 agtcgagtaa aatttgccct gtcataaaga accaataaaa aaggagttag ttacattgaa
     6961 ttatagaaac gatatccgta acgtcgcaat tattgcccac gttgaccacg gaaaaacaac
     7021 attagttgat gaactattaa aacaatcaga tacattagac gcacatacgc aattgcaaga
     7081 acgtgcgatg gactcaaatg cgctagaaaa agaacgtggt atcacgattt tagccaaaaa
     7141 cacagctgta gactataaag gtattcgcgt aaacatcatg gatactccag gacacgcgga
     7201 cttcggtggc gaagtagaac ggatcatgaa aatggttgac ggcgttgtgt tggtcgtaga
     7261 tgcgtatgaa ggaacaatgc ctcaaacacg ttttgtattg aaaaaagcac tggaacaaca
     7321 tatcacacca atcgttgtcg taaacaagat tgacaaacct tctgctcgtc ctgagcatgt
     7381 cgtagatgaa gttcttgaac tattcatcga attaggagca gatgatgatc agttagactt
     7441 tcctgtgatt tatgcatctg cactaaatgg tacgtcaagc ttgtcagatg atccagcaga
     7501 tcaagaacca accatggcac ctatttttga tacaattatt gaaaaaatcc ctgctcctgt
     7561 cgataacagc gacgaaccat tacaattcca agtttcatta cttgactaca acgattatgt
     7621 aggtcgtatc ggaatcggac gtgtatttag aggaacaatc aaagtaggag accaagtagc
     7681 attaattaaa ttagacggaa cagttaagaa attccgtgta acgaaattat ttggtttctt
     7741 tggactaaaa cgtctagaga ttcaagaagc aaaagctgga gacttgattg ctgtctcagg
     7801 tatggaagat atctttgtcg gtgaaacagt tactcctgtg gatcatcaag atgcgttacc
     7861 tatccttcac attgacgaac caacattgca aatgactttc ttagttaata attcaccatt
     7921 tgccggtcga gaaggtaaat tcgtaaccgc tcgtaagatt gaagaacgtt tgatggcaga
     7981 acttcaaaca gatgtatcct tacgagttga accaacaaac tctccagatg catggacagt
     8041 ttctggtcgt ggagaattgc atttgtctat cttgattgaa aacatgcgcc gtgagggata
     8101 cgaattgcaa gtatctcgtc cagaagttat tgaaaaagaa atcgacggtg taaaatgcga
     8161 accgtttgaa cgcgtgcaaa tcgatacacc agaagaatat atgggtagtg ttatcgagtc
     8221 cttgagctta cgtaaaggtg aaatgcaaga tatggtccat acaggaaacg gacaaatccg
     8281 tttgacattc ttaacacctg cccgcggttt gattggttat tcaactgaat tcctttcaat
     8341 gacacgtggt tacggaatca tgaaccatac ttttgaccaa tatctgccaa tgcttcctgg
     8401 tcaaattggc ggacgtcacc aaggggcgct tgtttcgatc gatacaggaa aagcaacgac
     8461 ttattctatc atgagtatcg aagaacgcgg tacagtcttc gtagaaccag gtacagaagt
     8521 atatgaagga atgattatcg gggaaaacag ccgtgataat gatttgactg tcaacatcac
     8581 gaaagcaaaa caaatgacaa acgtacggtc tgctactaaa gaccaaacat ctgtcatcaa
     8641 aaaaccaaaa caattgacgt tggaagaatc tttggaattc ttgaatgatg atgaatattg
     8701 cgaagtaaca ccagaaagca ttcgcttgag aaaacaaatt ttagagaaaa atgctcgtga
     8761 aaaagcatct aaaaagaaaa aataatctgt aaaaggatga agagagaaac cagcttgtta
     8821 taaaaaatag ctggtttctt tttttgtcca actcttttct gtggagaagt catctttgtc
     8881 aatggacata aattcagaaa atacgccgaa agacgtttaa aagactttta aaaaacttat
     8941 ttctagagag tttttgtttc ataataagat aatgtttcac gaaaggagat caacttgtcc
     9001 ttcgaagaaa atcgaagtaa aatggcaaga gaaaggagcg ggaagatgaa cgacttatta
     9061 aaacaatact ttggttatga tgagtttaga cctggtcaaa aagaaattat ccagaaagtc
     9121 attgatcagg aaaatgtatt aggaatcatg cctaccggaa gtgggaaatc aatctgttac
     9181 cagcttccag ctttactatt agatgggctt actgtggttg tgtccccttt aatctctttg
     9241 atgaaggatc aggtggacgc ggcgaatcaa ttaggtatac ccgccacttt tatcaatagc
     9301 tcgttagatg gttacgaaac agctagacgc ttccaagaaa tcgatagaca gcagtaccgt
     9361 ttactttata tcgctcctga acgttttatc atgccggatt ttatccaagc aatgaagcga
     9421 tggaacgtcc gtatgatcgc tattgacgag gcacattgta tctctcagtg ggggcatgat
     9481 ttccgcccaa gttatttaca gatggcgaac cagctagatc aactgccaaa tcgtcctgta
     9541 attgttgctt tgacagctac agcgactgtg caagtagctg ctgacataaa aagactttta
     9601 aaaattccag aaaacaacca tattcagact ggttttgaac gagaaaatct acgatttcaa
     9661 gtaattaaag atcaaaaaaa agaacaatat ctaatcgaat atttaaagat caacaaaaat
     9721 caatctggta tcatttatgc agcaacgaga aaagaagtag accgtctcta tcatttgctt
     9781 aaaaaattcg atttttccgt gggtagatat catggtggtc taaatgagaa tgagcgaaca
     9841 gaaatgcaag aagccttttt atacgatcgc ttgcagctga ttgtagcgac aaatgcattt
     9901 ggtatgggta taaataaaag caatgtccgt tttgttatcc attatcaaat tcctggctct
     9961 ctagaagcat actatcagga agctggtcgg gcgggaagag atggtctttc cagtgaagcc
    10021 atcctgcttt tctctcctca agatatccaa gtccaaaagt tttttatcca gcagtcccaa
    10081 agagaagaag ggcaaaagca aaaagaatat gaaaaattaa aagcgatgac agaatacgtg
    10141 tatattgagt cctgtcttca gcaatacatc ctaaactatt ttggcgagac aagcagtccg
    10201 tgcaaccgtt gtggtaactg tttagatgac agagaaatag tagaagtaac gacagaagca
    10261 cagatggttc tttcttgctt aaaacgtatg ggtgagaact atggtaaaca aatgctgatg
    10321 aaagtcttag caggatcgaa agaacaaaag ctgcaagctt taggttttgg acatttatct
    10381 acctatggac tgatgaaaaa ccaatcgcaa aaagaaacga tgcagttaat tgaatatttg
    10441 atatcgaatg gctatcttct gaccatcaat ggtgagtatc ctgttttgaa agtaacagaa
    10501 agagggatac aagtattgaa aggacaagaa tctgtttatc gaaaggaacc caagaaagtc
    10561 cagcagctat cggatgaaga aaccgataca ctatttgaag tgctgcggga actgcgaacg
    10621 gatctagcat cagaagcagg cgttcccccg tatgtcgtat tttcagattc cacattgaaa
    10681 gaaatgagcc gaatacgtcc atcaagcaga ttggagatgc ttcagataaa aggagttggc
    10741 caaagcaagc ttgataaata tggcgaagcc tttttatctc ggataaaaaa tgcggactct
    10801 aacgttctgg ataagtagaa agaacgaata cgtaaaaatg aataaaaaca actggttaaa
    10861 tctgtttttt ttgctcctta tgaaaatcaa atatgttata attaaggaat tagaatggga
    10921 ggtaagcaca gatggattct ttgtcgaaaa aacttgcgat ccaagtatta aatgatgaag
    10981 cagaacggat caaacgatta atccgaaatc aaaacaacag tctctgtatc tcacaatgca
    11041 aagccttcga agaagtggta gatacacaga tgtacggctt ttcccggcaa gtcggttttg
    11101 ctgtccgtat aggagtaatc ggaaagaacg agggccagct gatgttaagt gagctagaac
    11161 gagaattgaa tcagttatat agtgaggttt accaagaaat ttatgataaa aaagaagttg
    11221 gcaaggagga atagacttgc ctaagaaagt gaaaaaaagg catctattag actatagcat
    11281 cttgattccc tatttaattt tatgtgtatt gggattgatc atggtatata gttctacgtc
    11341 gtatctatta ttagaaaacg gacaaaatcc gtccgcatca gtcattaacc agagtatttt
    11401 ttgggtgctt agcttgattg ctatcgcact actttataaa atgaaaacag atgttttgaa
    11461 aaaccagcgt ttgatcatgg cagccatcgc agttttgacg attttgctgc tgattgttgt
    11521 gttttttggt aaggagatca atggggcgaa aggttggctg caaattgcag gattctcgat
    11581 ccaacctgcc gaatacctaa aaatcatttc tatctggtac ctgtctctga ctttatctaa
    11641 aagacaaaac agtgtacaaa aagattttct tgggactgtg aaacgtccac ttgcaatggt
    11701 tatcggactt actgcattag tcgctattct tcccgatttt ggtaatgcgg cagttatctt
    11761 tttgattatt ttggttctgc ttttagccag cggagtcaat tatgtgtata ctttgattgt
    11821 aggagtagga ggcttttgtc taagtacttt tacgatctgg ctgatcaata tcacaaacgg
    11881 aaagatctta cccggacgtt tacagtatat ctataatcga tttgctattt accaaaatcc
    11941 tttctcagac gaattaaata aagggcatca actagtcaac ggctactatg ccatgttcaa
    12001 cggaggacta tttggcaggg gattaggtaa cagtatccag aaaaaaggat ttttacaaga
    12061 agcacaaacc gattttattt atgcaatcgt agtggaagaa ctgggagtga ttatggggat
    12121 tttgatatta gcgttgctat tctttatgat tgtgcgcatt gtccttgtag gtatccgttc
    12181 aaaagatccg ttcaattcgc ttttgtgcat tgggatcggc gcaatgtttt tgatccaagt
    12241 atttgtcaat cttggaggaa ttactggcgt tattcctttg acagggatca cgttcccatt
    12301 tttaagtcaa ggaggctcca gtttgctgat gctttcgatt tgtgtcggct ttgtcctcaa
    12361 catcagtgcg gatgaaaaac gcaagagctt aggtttataa taaaaaaaga tgtaggagtg
    12421 gttcgatgaa aaaagtttta gttgctaaca ggggtgaaat cgctgtacgt gtatttcgtg
    12481 cttgtacaga attaggaatt aaaactgttg gaatctatgc agaagaagat gaatattccg
    12541 tacaccgttt caaagcggat gaagcatatt tagtaggaca aggaaaaaaa ccgattgatg
    12601 cgtatctaga tattgaaggg atcatttcta tcgcgaaaga atgcggagct gacgctattc
    12661 atccgggtta cggtcttttg tcggaaaact tgaattttgc gcaacggtgc gaagaagaag
    12721 gcatcatttt tgttggaccc aaactgcatc atttagatat ttttggagat aaaatcaaag
    12781 caaaagctgc agctatcgaa gcggggattg cttcgattcc aggcacagat ggaccaatcg
    12841 cttctataga cgatgccttg gagtttgcga aacaatacgg ttatccgatt atgatcaagg
    12901 cagctctagg cggcggcgga cgcggcatgc gtgtagctca cgatgaaaag agtgcaagag
    12961 agggctacga acgtgcgaaa agcgaagcaa aagcagcctt tggtagtgat gaagtgtatg
    13021 ttgaaaaata catcgctaat cctaagcata tcgaagtaca gattctcggg gatacgcatg
    13081 gaaacgtcat ccatcttttt gaaagagatt gttctgttca gcgccgtcac caaaaagtag
    13141 tagaagtagc gccttgtgtc tctatgaatg aacagcaacg tcaaaaaatc tgccaggcag
    13201 ctgtccaatt gatgaaatat gtcgggtatg tcaacgcagg tacagtagaa tttcttgttg
    13261 aaggagatga tttttacttt atcgaagtca accctcgcgt ccaagtggaa catacgatca
    13321 cagaaatgat tacagatatt gatatcgtaa caacgcaact gttgattgct caaggtttgg
    13381 atctgcataa agaaatcgga ttgcctcagc aagaaggaat taaattaaat ggttcagcca
    13441 ttcaatgccg aatcactaca gaagacccgt tgaataactt tttaccagac acagggaaaa
    13501 tcgatacgta tcgttcacct ggtggttttg gcgtacgtct ggatgtcgga aatgcttacg
    13561 cgggctatgt agtgacaccg tattttgatt cattgctagt aaaagtatgt acgcatggtg
    13621 ctacctttga aacagccatt cagaaaatgg aacgttgctt gaaagaattt agaatccgtg
    13681 gagtgaaaac aaacattcca tttatgctca atgtcatcac acatcctgaa ttccaatcag
    13741 gaaatgcaaa aacaaccttc attgatagta cagcaacatt atttgaattt cctcgactac
    13801 gagaccgcgg aaacaaaacg atgaaatata tcggtgaaat tactgtgaat ggttttccag
    13861 gtatcgaaag tggcgaaaaa ccattttatg aagaaccacg tatgcctaaa gatctaatca
    13921 caagaactga ttacgtaacg gctaagaatg tattagatgc gaagggtgcc gatgctttgg
    13981 tcgaatggat caaaggacag gaaaatttgt tgttgacgga tacgacattt agagacgccc
    14041 atcaaagtct gctagcaact cgtgttcgga caaaggattt caagcagatt gcacgtttga
    14101 ctggagaagg tcttcctgaa ctcttttcaa gtgagatgtg ggggggagca acatttgatg
    14161 tggcttaccg ctttttgaat gaagatcctt ggcaacggtt gagaaaaatt cgttctttaa
    14221 tgcctaatac gttgctgcaa atgcttttca gaggttcaaa tgccgttggg tattctaatt
    14281 atcctgacaa tgtattggtg gaatttgtca aagaagcagc agcgcaagga atcgatgtat
    14341 ttcgaatctt tgatagtttg aactggactc cacaaatgga aaaaagtatc caagctgtac
    14401 gggatactgg taaaatcgca gaggcagcta tttgctatac tggcgatatc aacgatccaa
    14461 gtcgggcaaa atacaatgtt cagtattata aagacatggc aaaggaacta gaacaactgg
    14521 gtgcgcatat cattgcaatc aaagatatgg ctgggctgct taaaccgcag gcagcttatc
    14581 gtttgattag tgaattgaaa gaaacgacag atctgccaat ccatctgcat actcatgata
    14641 ccagcgggaa tggaatcatc acttattctg ctgcttcaaa agcaggagta gacatcgttg
    14701 acgtggcaat gagtgcaatg agtgggaata ctagtcagcc tagcatgagc agcttgtatt
    14761 atgcactggt caatggtcca cggctgccag aaatcacgat tgaaaatgca cagaaattaa
    14821 accattattg ggaagatgtg cgcatgtatt acaaaccgtt tgaaaacggc ttgaatgctc
    14881 ctgaaacaga agtatatatg catgaaatgc caggaggaca gtattcgaat ctacagcaac
    14941 aggcaaaagc tgtcggatta ggtcatcgct gggatgaaat caaacaaatg tatcatactg
    15001 tcaacttgat gtttggcgat atcgttaaag taactccatc atctaaagta gttggagata
    15061 tggcactatt tatggtccaa aatgatctta cagaagaaga tatttatgaa aaaggcgaaa
    15121 cactaagttt ccctgaatct gttgtgactt tcttccaagg agaactaggg caacccgttg
    15181 gcggatttcc taaaaaattg caaaagatta ttcttaaagg cagacctgca ttgaatgaac
    15241 gtccaggtct tttggctgaa tcagtcgact tcaacgaagt aaaaaaagaa ttggcagaaa
    15301 aaattggcta tgaaccaaaa caagacgaag tattaagcta tttgatgtat ccgcaagtct
    15361 ttttggacta tcaaaaagca tataatcaat ttggcgatgt tacattactg gacacaccaa
    15421 catttttcca aggtatacgt ttgggtgaaa caatcaatgt acaaatcgaa cgcggaaaaa
    15481 cgcttatcat tcgtttagat gaaatcggag aacccgatat cgaagggaat cgcgtactgt
    15541 tcttcaattt gaatggacaa agacgtgaaa tcactatcaa agataattcg atcatcagta
    15601 cggtccaaac ccgattgaaa gctgaaccga caaaccgaga acaaattgga gcaacgatgt
    15661 ctggttctgt tttggatgta ttagtgaaaa aaggcgataa cgttaaaaaa ggtgatacgt
    15721 tgatgattac tgaagcgatg aaaatggaaa cggccatcga agcacgtttt gacggagaag
    15781 tcgcacatgt atatgtgtct tcaggagaca ctatttcgtc tggtgacctt ttgattgaag
    15841 tgactgagaa ataataagcg aaaggtgggg aaacatgaaa agactgggtt tgttttggct
    15901 gattctgcta ttagttattg tggcattcta catggaacca gtcttgtttc cgccaaaact
    15961 tgaagtgaag gaaaactctg atcaacaaat atcaaaccaa atgacaacgt ggaaatacca
    16021 ggagcttaaa gcaactggtt tttctactta tatcggaaaa ccgatcgatc agctggaaaa
    16081 ggattttggc caagcctatg atcgattaac tagcggattt ggttttgaaa caagatatta
    16141 tgaaaataaa gctagtcatc tcacatttga agcgaacatc caagacagca gagtcacaac
    16201 tgttaaagta ctgcaaacag ggcctgctga tatcgcgccg ttttatttag ggatgacgat
    16261 gcaagatctt acgaaaatca ctactatcta tactaatttc acttttgagt accatgatac
    16321 agaagttgga atcgaactaa tggaagagga tatgaattat cgtccgctaa ttgcttttga
    16381 taacgacact tttgctattt tattttttga tcaaacaaca aacggcctat tttcagttgt
    16441 gtatttagat aaagatagtc ttttgaaact attgccatat caagtgtttg gtgaaggcct
    16501 tccacattat caagcagata aatcggctga ttgggaatca atcaatcgcg ttaaagaacg
    16561 aaaaagtacg actttattaa atatgcttag aagagaagac gaacttccca tatacaatca
    16621 aacacttgcc tttcaaaacc acagtcagct gctactacat gattttttga caaggcctga
    16681 ggaaatattg tcagaggaac gtatggctga ttggcaaaaa tcgctaggca gtgctcaaac
    16741 gacagtgaaa ttctcactga cgaatgatga attggagaag ttaatcagta aacaaaaatt
    16801 agaaaatact agtgggctat ttattcatcc ggcgattgac gctacttttt cgtttttata
    16861 ttggtacagc gatccttaca accatgaccg tttcatggac ccgggaacgg attctgtagg
    16921 cattgctttt tctaaagaaa atatgttagt cttattgcaa gaagaaaacg gagaaagtag
    16981 tgatagcagt gatcgttaac gaagagctgt ttcaactgga ggatcaatgc ctccaagtcg
    17041 ctgaaatgat aaaaaagagt aaaacgatgg cttcttatct tgaacataaa cagtcaatgg
    17101 aagaatctgc aaaagtttgc cagttgaaag aagcgttctt gtctaagaaa gaagcatttg
    17161 aaaatgtagc ggcatatggt gagtatgcac ctgattatcg tgaaaaaaaa cgagctgttc
    17221 gaaaagcaaa aagacagctt gatatggatg agcatgttgc agctttccga tatagtgaaa
    17281 cacagctgca aagtctgttg gatcaaattg tccaagtact tgccgaagaa gtttctaatg
    17341 aaatcaaagt agatgcaggt agcccgtttt ttgaaacgaa aaaacacttt ggatgcggag
    17401 gtaattgtca tgcaattaga caatagtgaa tttgaaataa caaaacgacg cgggctgatc
    17461 gtatgggtct acagcttaaa acaattaaag aatctgaaaa agttcggctt gttgcattat
    17521 gtctcaagaa gaatgaaata tgtcgtgctt tacttaaatg aagagacatt ggaagaaaca
    17581 gaagaacgaa tccgtaaatt acattttgtt cgaaacgtag aacgttctta ccgaccggat
    17641 atcgaaatga attttgccga aaaaatagga aacaaaacag tattaaaaga cgaaggtttc
    17701 gaaattgaag agctaaatac agaaatccga ttagctgaac atctttaaaa atctcttatg
    17761 agcagttttt aaataaaaaa taacttggat aacgatcgat ttttgatcat tgtccaagta
    17821 tttttttggt atactagtac cagtaccatt tctaaggagt gagtagatgc ggataatatc
    17881 aggaaattat ggcgggcgcc gtttaaaaag tttagcaggg gcgaacacca gaccaactac
    17941 agataaagta aaagaatcca tattcaatat gattggacca tattttgatg gagagacagt
    18001 attggatctg tttgcaggaa gcggtggttt agctatcgaa gcagtatcta gaggctgcag
    18061 tcatgcggta tgcgtagaca aaaattacca agcgctaaaa atcatcaaag aaaacatcga
    18121 aattacgaaa gaaccagaga aatttaccac aataaaactt gatgcggaca aagcaatcga
    18181 acggctagca gcggagaaac aacaattcga ctacttgttt cttgatccgc catatgcaaa
    18241 acaaaaaata atcgatcaaa tccataaaat ggaagaactg ggactttttt ctaaaaccgc
    18301 agttatcgta tgcgaaacgg ataaaacagt cgaattgcca gaaactgtgg ctggattcca
    18361 tcaaataaga aaacagattt atggcataac ggccgtaact atctatagaa aagagggaaa
    18421 tcaatgaaac gtgcattatt cccaggaagt tttgatcctt ttacaaaagg tcacttagat
    18481 acggtggaac gtgcggctaa attattcgat gaggtagtaa ttggtgtttt tatcaatact
    18541 tcaaagaaaa gtttgtttcc cccagaagaa agaatgacat tgatcacaaa agctgtttct
    18601 catcttccta atgtaaaagt catgcatcaa gaaaatcaat tgacggtaga aacagcaaaa
    18661 gagattggtg cagatgcgtt gatcagaggc atacgaagta tcaaagactt tgaatatgaa
    18721 cgagagattg ctcagatgaa tcatcatcta tatgatgaat tggaaactgt cttcttatta
    18781 gcaaaaccag aatatagtca cgtgagttcc agtattttaa aagaagtact gcactttggc
    18841 ggagatgtga gcagctactt accgccagtg atcaacgaag ctttagctgg aaagcgtgaa
    18901 tcaaatgaaa gctaaacaat ggttgaaaac agtactgatt atcatatttg gaatgttgat
    18961 cattggattt gtagtagttc ccttgccgta ttatgtagaa tcaccgggag ccacgatcga
    19021 tctgaaagaa ctaattacgg tcaatgataa ggaagataaa ggttctgggt ctttctctct
    19081 tacctctgtt ggtatcaggc aagctacagg tttcttagct ttaaaagcta aaatgacccc
    19141 ttttgaagaa ttgatcagtg aagaagaatt gactggtggg gcgacaaatg aagaatataa
    19201 tcaaatgcaa cagtattata tggattcttc ccaaaatgca gcaatcgaac aagcgttaaa
    19261 acttgcagac aagccttata aaatggaatt caaaggcgta tatgtcatga gtgtagagaa
    19321 aacatcgaat ttctttggca agctttcagt tggagataca gtaacaaaag tagacgggaa
    19381 aagctttcaa agtacagaag catttatgga ttatgtaaaa agccaaaaag ttggacaaac
    19441 aatcgaaatc agttatttgc ataatggtga agaaaaaaca gcttctggtg atctgatcga
    19501 gttaccaaca gataaaaagg caggaatagg tatcacgctt actgaccata ccgagatcga
    19561 atcagataca gatgttcgta ttaattctgg ctctatcggt gggccatcag cgggattgat
    19621 gttcacttta gaaatctatg aacaagtcac tggtaaaaac ttgcgacatg gtaagcaaat
    19681 tgcaggaact ggaacgatca acagtgaagg agaagttggc cgaatcggag gaatcgataa
    19741 aaaagtggca agtgcggata aagctggcgt agagattttc tttgcaccag atgatacgat
    19801 ttcagacgat gttaagaaag aatatcctga aataaaaacc aactacgaag aagcaaaagc
    19861 tgccgctaaa aaattgaatt cctcaatgaa aatcgtacca gtaaaaacag tacaagaagc
    19921 gcttgactat ctagagaaaa tgaaatgaaa ctaaataatt aaagaggggt tgtgacacga
    19981 gttctgtcac aacccctctt tttcgtcaaa aatcttcacg ataaaaataa aaacaaaaga
    20041 aaaaattgac gtaattagat gttgaaggga cgattttgcc agagctcact gtttcttcaa
    20101 ttttaaatga aaggagtaat gaatgatttt tactggtcat tctttgaaaa aagaacaatg
    20161 atagaaaaaa tcagcaagaa aatactagct tcacccaaat tgatcatttg gatgtctatg
    20221 atagttctta tcttttgtat cgcaagtggt tttttattat caaatttgct attttataga
    20281 gaaacagaag agactgctgc aaaagaaata ctttcttcgt ttagttccag tggagaagat
    20341 ccatcagctg aaggaaacga tgtagaagag gatgctcgtc caaaggtcat gtacaccgat
    20401 atcaaaggaa gtgtgaagga gcctggtatc tattcctttt cttcagaaga acgggtctat
    20461 gatgtactaa agcgtgcagg tggattatta gaagaagcgg atagcgatcg aatcaacttt
    20521 tctgcgaaga tagaagatca gcaagtccta tatatccctg ctgtaggtga agagctgcca
    20581 gaacatcttg atcagttggc atcctcggaa ggaaaacagc ctacagcaga ttccgagcct
    20641 cccaaaataa atatcaatac agccactcca tcagaacttc agcagattcc tgggatcgga
    20701 tcagtcaaag cgcaagaaat cattcgattt agagaagaaa acggatcctt ccagaaagtg
    20761 gaagacctgc aggagatttc tggtatcggt gaaaagacag ttgaaaaact aaaaaacttc
    20821 gttacaatta aatagaatga ttcaaaatcg actggcacga gcaataaatg gacgctaatg
    20881 gatactaaga ataatccaat tctaagagct tggcaggacg aggaggtagt atttttggat
    20941 aggaaatagc ttgattgttc gcaaagaaac attaaaaaaa ccaatgctga atgtctatct
    21001 ccaaaataaa atatctggga ttcatattat gaatacggct gtcagcggca ataacagtca
    21061 agctctacga gaacgctttg ccaaagatgt acttagctat acagcagata aagtatttat
    21121 tctcattggg acgaatgatc tagcagagca taaacaactt tctaaagaaa cgtaccaaaa
    21181 aatctgcagt ggatgatagt tcagctgaaa aaagactata gttctgatca tgctttcttt
    21241 atcacgccac cagcagtcga tgagacaaaa caaaaatatc ggaataacaa gttgatcaaa
    21301 gaatatatct ctgttctcac agaagtagca aaacgccatg attgtgaagt atcgattttt
    21361 atacggaatt tcttgaacaa atggattcga agaattttta taatgggcta atggatgacg
    21421 gacttcattt cggcagtgaa gggtaggatc ttttgtcaga caggatgatt cgttatattt
    21481 aaagaatatt ttgtttaagc aatgagtact ttttaatcaa cttgaaaaaa cggaggaata
    21541 taaaacatgt tagaaaaatc ttcaaaagca tgcgatacga ataaagaaaa agttactgat
    21601 aagtcacata atgaacgcat tccttgggac cagtacttta tggctcaggc agtactgtta
    21661 tctttacgta gtacgtgtac acgtttagaa gtaggagcga cactcgttcg agaaaaaagg
    21721 atcattgctg gcggctataa tggagcagtc agtggagatg tccactgcat agatgaaggg
    21781 tgttacgtcg tggatggaca ctgcttgcga acgatccatg cagagatgaa tgcactccta
    21841 cagtgtgcaa aacttggtat cccgacggaa ggttcagaaa tctatgtcac acattttcca
    21901 tgtttggcct gtacaaaggc tttgttgcaa gctggtgtga aagggatcaa ttatctccat
    21961 gactatcgaa atgatcctta tgcccaagcg ttgatcgaac aattaggtgt atctgtacac
    22021 caagttgctt tagactcaca gcatttttca aaacttcaaa atgaattaac aggaaaacat
    22081 gtctagcgtt ttagaaaggc atgttcgaaa tcggttgatt tttccggcaa ttacgtcgat
    22141 gattatctgt ttgttgtatt acactgacaa ttggggttat cgtttgcttc ttgccggaat
    22201 ttttttatgg ttttcgctaa aagaaggacg ttttatcgcg tacttgtgta ttttgtgtag
    22261 cataacggta tccatttctt ataggctaca agagtattca tttttagagg aaaaaacaac
    22321 atctgcgcaa attactgtat ttacagatac gattcgtgca aatggtaatt ttttgacaat
    22381 ggaaggaagg ttatcagaga ataaacaaaa gctccagctt tcttatcaag taaacacaga
    22441 agcagaattg aaaacatggc ttaacttccg aggaaaggga atagaattga ctgctgaagg
    22501 cgagttcatc tctccagcaa aaaagcggaa tctttataat tttgaccaag aagcttactt
    22561 caggaaccat agaatttctg gacggttcca aattcaaaag attgaacaga tcaccatgca
    22621 aaatcactgg cgaaactggc ttcagagaaa aagaggatct tttatctcct ttgtccaatc
    22681 tgaatttccg caaaaaacaa gtgtatatat taattcttta ttatggggct ataaagatgc
    22741 aggattcaga gaaccagaag aaatttttag ggagagtggt ttgctgcatc tgttcagtct
    22801 atcgggtcta cacatccaat tttatttagg gtggctgtat tatttatttc gacgtatcgg
    22861 atggccacta cacgtcagta ttattccgtt gagtctacta actatctgtt atgtttctat
    22921 tgcaggaagt tctatttctg ttttgcgtgc tagtttgctg tttttattgc gattgctagt
    22981 caaattgctg aataagcatt attccaccat ggacttattt gcagttgtct tttggcttct
    23041 tttactattt gatcctcgat ttctttttca tgcgggtgga cagcttagtt ttttcatgag
    23101 ttttttgttt atcctgattt cttttgatgg agattggttg aaaaaaacgg cctctcacgt
    23161 cctgtttact atgatcacga tgccgttgat tgtatggcat ttttatgagt ggcctatttt
    23221 aggtagtctt tttacattac tgattgcacc ggtattcaag tttctttttc ttcctggtgt
    23281 gttatttctc acactcttca atcgctggct gccggaaact ttacttttgc tgatagaaga
    23341 gggattaatc ttatttgagc gaataatcga tttttcttca gggtcgtctc tcattatagg
    23401 acgtttgccc cttgtgctat ctgggatgta tattttcgga ttactgattt gtatggatag
    23461 gttgaaagaa caacctgtga aacacttgtt ttatgttggt cttttttctt ttattttttt
    23521 gactttgcca tttttacgct tttcttcaac gatcgctttt atcgatgtcg gtcaaggaga
    23581 cagtatcttt ttgcaaagtc cttttcgaaa agaaacgatt ttgatcgata ctggaggtca
    23641 gttacacttt gaaagagaaa agtgggcgca aggtatgata cgtcctcctg ctgaaagaaa
    23701 tatcgttcca tatctaaaag gtcaggggat aagtgtaatc gataaactca tcttgacaca
    23761 cgacgatacg gatcatgtgg gagaattacc cacgttatct cagcatttta ctatcaaaaa
    23821 aatttatatc ggttggggcg ctggacgaaa tgagccatta caaaggcatt tatctgaagc
    23881 tcaaaaaaat ggtactgaga tcattgaaat caaacatggg gacaggatca gcggttatta
    23941 tgatctttat gtattgactc cttttgaaaa gggagaggga agaaatgaag attcgattgg
    24001 tctatggatg gaatataaca ataggcgctt tttattttta ggagatttga atcaagcaat
    24061 ggaaaaacaa ttgcttttga tttatcctaa tctgaaagcc gatgtagtca aattaggtca
    24121 tcacggtagc cgtacgtcaa gtaacacaga ttttctttcc catattgaag caaaacatgg
    24181 gattatatcc tgcggcgtaa ataatcgtta tggtcacccg cacagcgaag tattggagac
    24241 actagaagaa aatcagataa aaacatggcg aacggatcaa caaggaatga tcacctatcg
    24301 ctggcatcca gtttttcatc ctcatgggct agtggaaaca atgatagact agtaattctc
    24361 atctgctcat ggtaggataa tttttgaaag tggtgagtaa tttgagtata caagcagagt
    24421 tacaaaaaat acgtaaagaa aaacttgcat cttgttactt gattttagga acagaaaagt
    24481 ttctccaaga tcaagttcgg acagaaattg tcaaaaaaat ccaattagag ggcgaagatg
    24541 acttaaattt tttaagtttt gacatggaaa atgcaagttt agatgaggta gtagctgaag
    24601 cagagacgtt gccgtttttc ggagatcaac ggttagtatt cgtagagaat ccttattttt
    24661 taacaggtga aaaagtaaac aatggaatcg aacaaaatac tgatttattg acggcttatt
    24721 tgaaagaacc attagaatcg actgtattag tattctttgc gccatatgaa aagttggatg
    24781 aacggaaaaa agtaacgaaa caattgaaaa aaacagcaat tatcatcaat gtccaacagt
    24841 tgaatgaaaa agaagtacgt caatatctga tgaatacctt agagaattcc tcaatcaaaa
    24901 tggaacgaag tgcaattgat ttattcttga ggttgaccga cttagattta tccaaaatga
    24961 tgagagagtt acagaaattg gtcctttatg gacaaaatca acagcaaatc acggtaagag
    25021 aagtagaaga attagttcct aaaacgttag aacataatat ttttgacatg acccaatata
    25081 ttttaaaagg gaagacggaa caagctttgc gtttatacga agatttagtt atgcaagggg
    25141 aagaaacaat caaaattaat gcgattttgc tttctcaatt acgtttattt ttacagacaa
    25201 aatttcttgt aaagattggt tatcagcagg caaatattgc tgaaacattg aaaatccatc
    25261 catatcgtgt aaaacttgcc atgcaggaag taaaaaaatt cgatgaacat ttgctggtac
    25321 agttgtttga tcaattagtt gagatggatt atcaaatcaa aacgggacaa atcgaaaaag
    25381 aattaagttt tcaactattc gttttgcgca cagggcagtt gttaagaaat cgataagaag
    25441 aaagacgagg gcaaagcatg cgggaaggtc gtttgttttt gtcctttttt tgacaaataa
    25501 aaaaatcggc cgaagccgat tcttttattt agcgattttt ttgcttaaac gagatttgtc
    25561 gcggttagct ttgtttttgt ggattagacc ttttgattca gccatatcaa tagctttaac
    25621 agcttcttta tataacgcat ctacgttgtc agcacctgaa gctacagcat cttcaaattt
    25681 tttgattgca gtacgcatag cgcttgtttg agatgaattt ttcacattag cgttttcgct
    25741 tgtacgaaca cgtttgattg cagattcaat gtttggcatt agtttcacct ccgataactt
    25801 aagttcttac gcgtgttacc gcataattca acattcatta ttatacctaa aggcagcgtg
    25861 ctttgcaata tgaacatgac aagttttttt ccttaacagt cttttattag gatttttttt
    25921 cagtaaaaac ggatagtaca agctgaaaaa aatcaaaaag acgctattgt gggaggcaat
    25981 agcgtcaaac caaactagat ttttatttat ctatttaaac aaagtttgaa tgggaatgtt
    26041 tggtactaag ttattatata acaaagagaa ccgtttgcaa agtttttttt gaaaagggat
    26101 acgattccct gttgttcttt gtttttctat taagaaactg cagttatttt ataaccagta
    26161 tcctttgatc agtaaaggct attctggtgt accgattatt ttaatgtatt catcgatatt
    26221 ttttatcaac atttttttta tgaccaactg gttccgacgg ttccatttgc cacttacttc
    26281 cagcacatat ttatttaggg gcaaacgtaa gatatatttt gccaaatagc gatctttaag
    26341 caggccattg atcacacggt cctcaagctg caagttgatc ccagtcagca tcattggctg
    26401 ttcttgtaaa acgtacgtat caattaaaag accttcaaaa gtttccacaa gcgtcttcct
    26461 ttcttttcaa agtaacctga aaacaggcac taattcttcc attttgatat tcgttgaagt
    26521 ggatgcgatc attctttctt ttgtccactg taatctagta taacaaaaaa gagcagaaga
    26581 gccaaaaaga aaaaagagca tcgaattcta taggaagtct cattggcacg gcctaaatta
    26641 ttacaaaaca ttgattaaaa aaagcccctg catcgcagga gcatccccta tatcgtttta
    26701 ataggtcaat caaaaagcag tatcaaacac attttgtatc aaatcaggaa ataatagata
    26761 agctaagccc agaatcaaaa gtaccgctag aagaaaccag ccgattgttc ctatgaaata
    26821 ctttaatttt tcgaacattg tatcacctcg ttgtcagtat atcacggatc agtaaattgg
    26881 gggtgttaag aatctcctaa aatagacaaa atttgttcta ttttcctaaa gcaaatactt
    26941 gtaagataca gtaagtgata ccagaagcaa tgaccggtcc ggcggcgatt cctcttaaga
    27001 agacaacacc taaaatcgtg ccgaaaacaa gtgcaacagt gatttgtgga tcagaagcaa
    27061 tcaatcctac acccttggaa gataagacag ccactagccc accgcaaaaa acagctaccc
    27121 agccaatagg ggatttaaag gcttctaata gatcacgtag accgatttct cctgttgcga
    27181 tagggattag aatagccgct gtgatgatcg tcaccccaat attgattcct ttgccttgaa
    27241 gccaagggaa aaaggaacgg gaggctggta tgagcttcat aatcaaaaca aaagctgccg
    27301 ctatcatcag actttgattt ttagctaaaa ttgctacaag taaaataatt cctaaaaaaa
    27361 gccaactttc catgaaatat attcctcact ttcttcgaaa caatcataac atgtttttag
    27421 aaagttacaa aagtaggaat tcatccttca agacctattc ataagctgtt tttttcataa
    27481 aataaacaaa atcataaaaa aacactttgc ttatttgaca gaagacagcc gcgctttgta
    27541 tagtggacaa gacttacatt tttttgaaga atcagaaaga tcaggtaaag gtcttagtaa
    27601 acgcaagcca ttcagaataa ccaatattgt gcttccttcg tgtcctacaa ctccaagagg
    27661 tagatttaaa aattgcagga agttagaagc aatcaacaag agaataacaa gaatcgaaaa
    27721 aataatattt tgtttgatga tccgccgcat ttttaaagat agccgatgac tgtagacaaa
    27781 tttatttaag tcgcttttca ttaagacgat gtctgcgata tccatagcgg catctgtccc
    27841 ttgccccata gcgacaccta aagaagcggc tgctaaagca ggcgcatcat tgactccatc
    27901 accaaccatc gcatttactt ggtaaagttc attctctttt ttgactagtt gtgttttttc
    27961 ttccggagta cagcttgcat agtaatgttc aatccctact tgttcagcaa tggcctttgc
    28021 tgtgccagaa tggtcgccgg ttatcatact tgtatggatg ttttgggatt taaagtagga
    28081 aattacttgt tgggtatttg cttttggaat atctagcaga cctaaaacag caattaactg
    28141 ctgatcttta gaaagataaa tcacggtttt gccttgattt tccaaacgtt cgatcatttc
    28201 cgcaatctct ttgctgatta tcatcgtctc aggatcatat gcgtgcttgc caactttcca
    28261 ttgcgcacct aaataggtag tttgtaatcc aaaaccagta atttcttcta ttggtaagta
    28321 ttggatttct tcaggtattg tgcaatcgaa atattgcaaa atcgcatgag caagtggatg
    28381 cgtcgttttt tgttccatag caacaagaag ttgttgtgcc aaatcctttt catcaaagaa
    28441 aaagctgtca gttacaactg gttttccttt agtgagcgtt cctgttttat caaaggcaat
    28501 cgcttttaat tcagaaagct tttctaaatg gataccgcct ttgattaaaa tcccattttt
    28561 tgcagaattt gaaagagcag ctagagaagc tggtgtagca gatgcgacaa gcgcacaagg
    28621 agaagccaca actaataata ccattcctcg ataaaagctt tcatcccaac tccaacctag
    28681 gaaaaagtgg gggagaagga tcattagagg aacagcaagc aaaatcaatt ttacataatt
    28741 attttcgatt cgttcgataa atgaagctgt ttgagttggc gtattctgcg cttcttctac
    28801 taattggata atcttagcaa agacagtttc ggaactcgtt tttgttactc gaatcgtgat
    28861 gggatttcct tgattcagcg ttcctgcata catctcatcg cctaaatatt tttctacagg
    28921 cacagattca ccgcttaatg cagactcatc gatcgttgcc tgtgtgcttt ctaaaaagcc
    28981 atctatcgga acagctgctc ctttagcgac aaataccaag ttatccacct ttagttgaga
    29041 aacatctact tcttgcattt gtccattttc ttttaataat tgggcttttt gtggttgaag
    29101 attcatcaaa gcagtaattt ctttttgact tttattagtt gtgtattcct ctaatgcacc
    29161 gcttaaacaa aagatgaatg tcaacattgc tccttcaaag taattaccga tcaaacaggc
    29221 accaatagca gctaaagcca tcaataaatc cacattcagt gtccgatcat tccacaattc
    29281 tttcaagcct tccgatgttt gtttatatcc accgatgata atggcactga tataaaaaag
    29341 tggggtgatg gtaagagaga atcggtcgaa gataaaaccg aagatcataa ataaaaggca
    29401 gccgattgtt gctgcaaaag cttttttctg attagtcatc gttattagac ctccttgtca
    29461 aaacaaggat agcataataa aaatgtaatt gataataata acagctaaat gagaacgata
    29521 atggttatca tttggtgatt aagcttgatg ttgtttttat tatttataat gattattatt
    29581 aaagaaaata aaaaaccaga acatttctca aataagag
//
LOCUS       DACDSR010000025        28418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDSR010000025 DACDSR010000000
VERSION     DACDSR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.025"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..255)
                     /locus_tag="IWK15_001167"
     CDS             complement(<1..255)
                     /locus_tag="IWK15_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546784.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRY"
     gene            complement(504..704)
                     /locus_tag="IWK15_001168"
     CDS             complement(504..704)
                     /locus_tag="IWK15_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9546785.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1009..2241)
                     /locus_tag="IWK15_001169"
     CDS             complement(1009..2241)
                     /locus_tag="IWK15_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9546786.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2495..3064
                     /locus_tag="IWK15_001170"
     CDS             2495..3064
                     /locus_tag="IWK15_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9546787.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3242..3682)
                     /locus_tag="IWK15_001171"
     CDS             complement(3242..3682)
                     /locus_tag="IWK15_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9546788.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3840..4604
                     /gene="map"
                     /locus_tag="IWK15_001172"
     CDS             3840..4604
                     /gene="map"
                     /locus_tag="IWK15_001172"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9546789.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4636..5559
                     /locus_tag="IWK15_001173"
     CDS             4636..5559
                     /locus_tag="IWK15_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9546790.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5635..6774
                     /locus_tag="IWK15_001174"
     CDS             5635..6774
                     /locus_tag="IWK15_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9546791.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6767..7567
                     /locus_tag="IWK15_001175"
     CDS             6767..7567
                     /locus_tag="IWK15_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9546792.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7567..8394
                     /locus_tag="IWK15_001176"
     CDS             7567..8394
                     /locus_tag="IWK15_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9546793.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8372..9106
                     /locus_tag="IWK15_001177"
     CDS             8372..9106
                     /locus_tag="IWK15_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9546794.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9206..10072
                     /gene="rfbA"
                     /locus_tag="IWK15_001178"
     CDS             9206..10072
                     /gene="rfbA"
                     /locus_tag="IWK15_001178"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9546795.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10086..10658
                     /gene="rfbC"
                     /locus_tag="IWK15_001179"
     CDS             10086..10658
                     /gene="rfbC"
                     /locus_tag="IWK15_001179"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9546796.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10680..11708
                     /gene="rfbB"
                     /locus_tag="IWK15_001180"
     CDS             10680..11708
                     /gene="rfbB"
                     /locus_tag="IWK15_001180"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9546797.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11806..12657
                     /gene="rfbD"
                     /locus_tag="IWK15_001181"
     CDS             11806..12657
                     /gene="rfbD"
                     /locus_tag="IWK15_001181"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9546798.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12692..14725
                     /locus_tag="IWK15_001182"
     CDS             12692..14725
                     /locus_tag="IWK15_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546799.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14769..16049
                     /locus_tag="IWK15_001183"
     CDS             14769..16049
                     /locus_tag="IWK15_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9546800.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16259..17065
                     /locus_tag="IWK15_001184"
     CDS             16259..17065
                     /locus_tag="IWK15_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9546801.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17077..18297
                     /locus_tag="IWK15_001185"
     CDS             17077..18297
                     /locus_tag="IWK15_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546802.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18287..19873
                     /locus_tag="IWK15_001186"
     CDS             18287..19873
                     /locus_tag="IWK15_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9546803.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19912..22050
                     /locus_tag="IWK15_001187"
     CDS             19912..22050
                     /locus_tag="IWK15_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9546804.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22418..23401)
                     /locus_tag="IWK15_001188"
     CDS             complement(22418..23401)
                     /locus_tag="IWK15_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9546805.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23779..25170
                     /locus_tag="IWK15_001189"
     CDS             23779..25170
                     /locus_tag="IWK15_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9546806.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            25186..26226
                     /locus_tag="IWK15_001190"
     CDS             25186..26226
                     /locus_tag="IWK15_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9546807.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26245..27330
                     /locus_tag="IWK15_001191"
     CDS             26245..27330
                     /locus_tag="IWK15_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9546808.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27363..28325
                     /locus_tag="IWK15_001192"
     CDS             27363..28325
                     /locus_tag="IWK15_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9546809.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT         9195 a         4689 c         5700 g         8834 t
ORIGIN      
        1 ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc acgatatcaa acaaaaagta
       61 tacacccttc ttcattctag ttttcgcagc aggaattttg aaatagttct tattagaata
      121 gtagagattg attaataagc tatcttcgat tgctaaaaag aaaacttctg aattccatac
      181 tttataaaaa tctttgataa atctattcga agggtcaaat ttaaaccata attgtgtttt
      241 tccttccatc agcataaaag ttcacctcaa aaagagtata cgaacgaatg ttctttttgt
      301 gaaggcggaa tttaatatac tgaataatag aaaacaagta cttgtgccaa attatgtgcc
      361 aaaaaaattc gaattcaaca aaatgtaaac aaatagaaaa ccttctatat taagatttcc
      421 tgcaataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      481 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      541 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      601 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      661 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      721 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      781 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      841 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      901 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      961 ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc
     1021 gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt
     1081 gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa
     1141 ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc
     1201 taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga
     1261 aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt
     1321 atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc
     1381 atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt
     1441 acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg
     1501 catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg
     1561 gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa
     1621 ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc
     1681 taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc
     1741 aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc
     1801 agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc
     1861 tttgccattc gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc
     1921 gggatcggca gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg
     1981 atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc
     2041 tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc
     2101 ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt
     2161 gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag
     2221 ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt
     2281 agcatgatac catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta
     2341 tttttttaag gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa
     2401 aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg
     2461 aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag
     2521 agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac
     2581 tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa
     2641 aaatatggat gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga
     2701 agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta attatatcca
     2761 ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg
     2821 aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc
     2881 ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc
     2941 tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga
     3001 aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga
     3061 ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga
     3121 aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg
     3181 caatgaataa aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat
     3241 attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt
     3301 cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag
     3361 cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc
     3421 agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga
     3481 tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct
     3541 cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat
     3601 tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat
     3661 atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga
     3721 attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt
     3781 tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata
     3841 tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag
     3901 cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg
     3961 aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg
     4021 aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc
     4081 gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag
     4141 gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc
     4201 gattgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga
     4261 atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga
     4321 tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc
     4381 ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg
     4441 aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt
     4501 atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg
     4561 gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag
     4621 gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc
     4681 tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc
     4741 gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta
     4801 ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag
     4861 accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc
     4921 ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg
     4981 caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt
     5041 ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt
     5101 cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac
     5161 tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc
     5221 atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg
     5281 ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct
     5341 aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg
     5401 ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt
     5461 caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc
     5521 aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa
     5581 atttatatca gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt
     5641 gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca
     5701 cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga
     5761 ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata
     5821 tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt
     5881 gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa
     5941 caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc agatatacgt
     6001 attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc
     6061 gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat
     6121 gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt
     6181 ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt
     6241 atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct
     6301 ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt
     6361 gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct
     6421 atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat
     6481 caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg
     6541 gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc
     6601 gtattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc
     6661 aatgagaaac atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt
     6721 acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt
     6781 aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact
     6841 agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa
     6901 caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga
     6961 tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc
     7021 tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt
     7081 tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat
     7141 tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta
     7201 cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt
     7261 gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag
     7321 ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt
     7381 catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt
     7441 gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa
     7501 actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt
     7561 tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa
     7621 tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt
     7681 tacgataata gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat
     7741 catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca
     7801 gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat
     7861 tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt
     7921 ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct
     7981 caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt
     8041 gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat
     8101 tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt
     8161 cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga
     8221 taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta
     8281 tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac
     8341 cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt
     8401 gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag
     8461 tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga
     8521 tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc
     8581 ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga
     8641 tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac
     8701 tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga
     8761 cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg
     8821 gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt
     8881 aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt
     8941 gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga
     9001 tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt
     9061 tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa
     9121 atccttaata tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg
     9181 aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac
     9241 acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc
     9301 aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat
     9361 ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg
     9421 actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat
     9481 tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta
     9541 tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt
     9601 atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat
     9661 gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg
     9721 cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg
     9781 tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt
     9841 agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga
     9901 agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga
     9961 agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc
    10021 attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct
    10081 tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct
    10141 ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg
    10201 gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc
    10261 gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg
    10321 gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg
    10381 acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc
    10441 atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata
    10501 atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa
    10561 ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat
    10621 ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta
    10681 tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg
    10741 tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa
    10801 acaaagagaa cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg
    10861 cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg
    10921 cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca
    10981 ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat
    11041 ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag
    11101 gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta
    11161 caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa
    11221 ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc
    11281 gccaagtaac gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg
    11341 ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag
    11401 gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga
    11461 tggaattgat cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc
    11521 gcgcagggca cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt
    11581 ggcagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag
    11641 aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg
    11701 gacaataata cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct
    11761 cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact
    11821 ggaggaaatg gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca
    11881 tatgtttcaa cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat
    11941 attacagaat taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa
    12001 gcagaagaag aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta
    12061 gcacttgcag caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc
    12121 gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa
    12181 tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat
    12241 atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa
    12301 cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact
    12361 tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt
    12421 atttatcatt tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg
    12481 aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca
    12541 cgtccgcagt attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca
    12601 acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt
    12661 cctttttatc aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg
    12721 aaaagaaaag ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa
    12781 actccgctat cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg
    12841 aagttcactt ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga
    12901 tgtgattttt ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt
    12961 ttacctctca caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg
    13021 acaaaacatg atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc
    13081 attcaactgg ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat
    13141 gaaattagtc gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa
    13201 aaaagctcta tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg
    13261 gttcatgcaa catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta
    13321 tcattatttc tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt
    13381 tattttcggt attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta
    13441 cttgctgatt ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga
    13501 tctgttggat gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca
    13561 tttctggcta acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa
    13621 acgggtaagt actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg
    13681 gaaaattcct tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca
    13741 cttttttcca actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga
    13801 acaaaagtta ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac
    13861 cgttgggttg ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag
    13921 agccatcctt acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat
    13981 ttggagagaa gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg
    14041 gtcttatttc tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac
    14101 catcgcagtt gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg
    14161 cttgttttac ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt
    14221 cgtagaaggt acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga
    14281 tcaacaagat cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac
    14341 attgggagtg aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa
    14401 aatcgatccg aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt
    14461 tatcgctaag gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt
    14521 gaattttgaa gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac
    14581 tgattacaac caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg
    14641 ttatatgatt tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca
    14701 ggaaaatcag ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag
    14761 aaaggagagt gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg
    14821 gtttcttgct tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc
    14881 cagcaacttt gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt
    14941 tgctcatttt aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc
    15001 ttgccctttt cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag
    15061 acttactgtt tcctttggtt attttatttt gtttagtaat taaatggatc gagtggacaa
    15121 aatgggatcg cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc
    15181 gtgtatttac agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc
    15241 tttctgatat ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat
    15301 tgatcagtgg atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag
    15361 cagtcgttgc agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt
    15421 tgattgtttt tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc
    15481 catttattgc ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg
    15541 tctctgaaaa agtcaatttg tttaccgatc gagaagatat ttggcataaa ttctatcaga
    15601 cgctaggaga aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag
    15661 gaaaccaatt tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga
    15721 taggatttgg gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag
    15781 cagatttatc caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt
    15841 catttatgga ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg
    15901 ctatggccgt tcatcgatat gatgaaccag aagttgcgga ggaattctat gttatggaag
    15961 aggcatatga cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc
    16021 attcttccag gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa
    16081 ccgtcgcagc cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt
    16141 tagagaaaat cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa
    16201 aaatatggta aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat
    16261 gtttaaagat ttgtatttat ttatgaagga catttatcag aacagaaaat tattgctaca
    16321 gttttcgatc aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc
    16381 gtttatcaat ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa
    16441 agcacaaatg atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc
    16501 ctggttcttc ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta
    16561 tttagtaaag aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa
    16621 tctgtacacc catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat
    16681 atatccttca gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt
    16741 gacaggattg acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt
    16801 aattacgatc gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca
    16861 gtttgattca ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg
    16921 ttatcgtgag tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta
    16981 tttctgggtt ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa
    17041 accacatttt tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta
    17101 aaagatatca caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc
    17161 ctcaatcctt ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta
    17221 gaaataaaaa aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca
    17281 atgttgaaaa tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga
    17341 aaaatctcag ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa
    17401 aatatctatt taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc
    17461 gatgatatca ttgaatttgc tgacatcgga gaccatattt atcaacctgt aaaaacttat
    17521 tcaagcggga tgttcgttcg tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt
    17581 ttgattgtgg atgaagcatt ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa
    17641 aaatttacag aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatatcagt
    17701 tcgattcgtc gtttttgtga tcgttcctat tggttgaaaa acggagaagt cgtcgaatat
    17761 ggcgatacga tggaagtaac gacgaattat gaaaactttt taaagaaaaa atcagttaaa
    17821 acagtggatc ggaataaaaa ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg
    17881 acgaaagcgg aattgttgga taaagacaaa aatccggtgg atatgctgga acaaggagaa
    17941 aaactatacg ttaaagtaac ttatgaagta aaagatgata caatcaaaaa tccggtttta
    18001 ggaattgcgc tacgaacagt cgacaatttt tatgtatgcg gacttaatac tctactagat
    18061 ggtatcaaga ttccttggaa aaaaggagag aatatctttt atctggagta tgaaaagtta
    18121 ggtttgttag ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt
    18181 cctttggtat ataagacaaa gtacatgaac ttgtttgtca gcggttctta tatcggagaa
    18241 ggcatcgtgg tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt
    18301 tgaaatggac ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg
    18361 aagtaaagtt ttagagtttg gtcccggaaa cggccgatta acgaaacatt taatcggagc
    18421 aaaacaatgt gaagtgagca ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga
    18481 atttgctcaa gatggatttt atggtgatat cgaaagtttt gagtgggcaa actattatgc
    18541 tggacagaca tttgattacg tcttgtttgc ggatgtttta gaacatttgg tagatcctgg
    18601 aaaaacattg aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt
    18661 ccctaacttg gcgcataatt ctgtcatgat cgatttgttc aataaccaat tgccgtgggc
    18721 ttcttatggt ctcctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa
    18781 agtttttgaa aaagcgggat tattcattaa tattgaagat tatttgtact tagctgtagg
    18841 tgatacagaa ttaaaatcaa cttatgaaga attgccggaa gctgtacgct atgatttcaa
    18901 aatgcgacca tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagc
    18961 acaaagttcc attgcagagc cacaaaattc taattatgta aaagtgttag aagtaaccca
    19021 gcaaacgaaa caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa
    19081 tgagacacta agtttccccg tatcagaaac aacagaaaga atggtgtttc gcttcgcgca
    19141 acaacctagc tttatcgaat tcagcgcaga agctgccgga gaaaaactaa catttatcga
    19201 ttcgaatgct gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaattacc
    19261 agagttcgtt ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt
    19321 tatcggggaa ttgacaccaa caatgaaaga attgttggaa acgattcgtc ccatggctga
    19381 agtcacaaag cagctttctg agaaaaatga cgaattagaa agagaaaatc atcatcttct
    19441 tgaggaaaat acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt
    19501 gttggaaagt gatgaatgga caataaaaca tagactagga cgacgtaaga aagaaacttc
    19561 gaaaaaaatc caagaaaaag aactttctat atgtattgac gagaaaatct gggatgcaga
    19621 aacaaaaata ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agcccttgtc
    19681 ctataagtta tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagaaga
    19741 agtcaatcaa gcagaacagc tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct
    19801 ttgcgagcaa gaacgttcat tcttagtgga agcagtagct caaaatggcc aatcatggat
    19861 cattgaaatg taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa
    19921 aaagaaattg ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt
    19981 attgtgggct gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa
    20041 gaaaatgtga ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat
    20101 cttgatgtta agacccaaag cggtttcaag gtacgtttat cagggaaaat gagaggaaaa
    20161 gccatcttag acttccaaac agcgagacat caaaatggca tagctgtgaa attaaacggt
    20221 aaataccctt atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa
    20281 aaagggataa attatgcaag gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg
    20341 gaattgaagc caggatcgat cgactatgca gagtggatca gtcgtcatga gaaaccagat
    20401 ttgaaagagc aaaggaaaga agcacaagga tttgcgtacc gtccattgat ttccatactt
    20461 atgcctgtct ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtactggat
    20521 caaacgtatg atcattggga actatgtatc agtgatgatg cttctactga tccagctatc
    20581 cgaaagtgtc tggaatccta tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa
    20641 gaaaatggac acatcagtct ggcaactaat tctgcattgg aaatagcaga aggagaattt
    20701 atagcactct tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa
    20761 gtgctaaacg tacatccaga actggatgtg atctacagtg acgaagataa aatcgatgca
    20821 gatggcaatc gctttgatcc gcatttcaaa gcagactggt cgcctgatac attgatggga
    20881 aataattata tctctcactt aggcgtatat cgaacaagca ttgttaaaga acttggcggt
    20941 ttccgtaaag gctatgaagg ttctcaagat tatgatttgg tccttcgtgt gacagaacaa
    21001 atccctgcag atcatattta ccacattgac cgagtcttgt atcattggcg gacgatccca
    21061 ggatcaactg caagtaatgg cgaagccaaa agttatatct atgattccgg agtgaaagca
    21121 ttgacagatg ccctgtctag aagaaacatc aaaggaagcg tacgtcctgg cagaatttct
    21181 ggattttatg agattgctta tgacgtcttg caagaggatt tggtcagtgt gatcattcca
    21241 acgaagaatg gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct
    21301 tattcaaatt atgaaatcat tattgcggat aacggaagta cagatccaaa aatgcaagaa
    21361 ttatttgcag aatacaagca tcagctgaaa gaccgcttta tcgtagaatt gattgacatt
    21421 ccattcaatt attctcgaat taacaattta gcagcagaaa aagcaaacgg caaatacttt
    21481 ttattcttga ataacgatac agaagtgatt gagccagatt ggatgacggt gatggtttct
    21541 tatgcacagt ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca
    21601 acgcagcatg caggagtatt attaggaatc ggtggtgttg cagggcatgc attgaataac
    21661 tatgatcgaa cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca
    21721 gtcactgcag cttgtatgat ggtcaaggca gctgacttca atgctgttaa cgggtttgat
    21781 gaaacattag aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt
    21841 cgttacaatg tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt
    21901 tatgaagata ctccagaaaa acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat
    21961 aaatggccag catatattgc gcatgatcca ttttacaatg ataatttaac aaaagacgga
    22021 atcggcgact tttcgttgag accagattaa actataataa aaagctgtta tgttgtgatt
    22081 cgctcgcaca taacggcttt ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc
    22141 aaaaaaataa gtcgaggttg ggacagaagt gtttaactcc gagaaataag aagaaattca
    22201 cgaaaattgc ttttcaaatt tttgtgaatt tcggcttatt taagaagaag ttgcttctgc
    22261 tcccaccgtt tattcgtttt taaagaatga gacaaaagtg tttcttactt ttgtcccacg
    22321 ctcttattca tgctcagagc gccaatagat acaaaatcat agccaaagtt tcacgaaccc
    22381 tagctgtcaa agaatcgata ttttatttta atataggcta ttttaaaata aatggtttaa
    22441 ggtattcctc tacaaaagcc actctatcaa ttatcatggg tttaaaataa gaagtgatgg
    22501 agataaccag attgttctta gcgtcaatat aaagaacatt tccactatta ccaattgctg
    22561 aatatatttc gttctcagaa tctaataccc accataaata gccatatctc atattttgta
    22621 agtcttctaa agcactggtt ctaggtttga acatctcgtt tatccacatg ggtggtacga
    22681 tttgttttcc tttatacatc cctttattta ggcataatac accaattttt gccagatcac
    22741 ttgccgataa acacaaacca tatcctgccg tcccgatatt tagtggatca caaaaccaga
    22801 cattggtttt aggagactta tccattgtaa actgcttatg ttcttcagct gtttcagcat
    22861 aataattgac atggtttggt atccctatag gatcaaatag atacttattg gcaaaatcaa
    22921 cagttgttag cttacttgtc tgatacaaca agccagttaa tatgtgtaaa cagacagttt
    22981 gatagttgaa ttcgccggtt ataccttttc ttcctcccaa taaatctagt gatgtttttg
    23041 tccaatcttc acttgaacag acttttgtcc atggatcgcc tttacatttg tagggtgctt
    23101 tcattgttag caagtgcttg attgtaatag cgtaaatagt tttctccccg cgtttcactt
    23161 tatactctgg aaaataatct aatactttat catcgatact tttgattaat ccttgctcta
    23221 aagcaatgcc aatcagcaaa gatacaatac ttttcgttgc tgacatgata tgacaagtat
    23281 cgtctttttt ataaccattc cattcatctg tataaacttc taccccatcc ttatatgcga
    23341 caatttgaca tatatttggt tgtcgaaaac caataaaatc atgcaactct tgtttattca
    23401 taagatatta cctcactttc ttctataagc ataatgtcat taaaaatcga tttcaatttt
    23461 ttcgattctt cacgtaatac aggtattctt atggactaga cagcgctttg ggaagatgga
    23521 ttctctgaaa aatataaagg ttcactttat gagtggataa aaaaagcagc aaatacttcg
    23581 acttctttaa tttaccaaca ttagtgaaaa tcgattcatc tggagatttg taatttagag
    23641 tccttgtctt gattagaggt gtataaagct ttttattaga catttttaaa ttccattaca
    23701 atttgcttat aaactaggtt tttaaaggtg actatggtaa gataatagag atattatttt
    23761 gtgattagga gtgtatatat gaataaaaat ggagaatgga gtgctgcacg acggatattc
    23821 attatattaa tggatgttat tgtatacaat gcagcaatat atagtagttt cttactgaag
    23881 tttcgtggag agatcccatt acgaaattta gaaactttcc aacactcagc aattattatt
    23941 tcagtgattt ttattgcgtt gaacattttg ttgggtgcat atgtttttta taatcgaatg
    24001 atcagtgaca tcgtgtttgt cacagtcatc ggtcaggtat tgatgacact agggatcatg
    24061 gtagtgactt ttgcgggccg ctggtttgct tttccacgtc ttgttatctt actttcattt
    24121 gttctcggta cgcttttctt aagtatctat cgtattctcg tatataaact ttatttacgc
    24181 gtaagtcgag ataaaaaggt cgtcattatt gggttagaaa aggatgtcgc accagcaatc
    24241 caaaattttg gttcaaagaa aaacaacaaa cataaagtcg aagcagtcat tcttgataac
    24301 tattatgcaa acatcaaaaa tatgattaaa gacatcgata ttgtctatct tgcttctcat
    24361 atcgaagaaa ctgaaaaaat caaaatctat caattagtta ctaaaaatga aaaaaaattg
    24421 tttttgaata caacatttga aaacttgaca ttgatcaatc caaatattat gaatttcgaa
    24481 gatgaaagta ttattgaagc atctggattt agaatccaac ctgaatatga actattcaaa
    24541 cgtttgtttg atatcatcgt ttcacttgtt ttattagtta ttgcttcacc atttatgtta
    24601 ttcacagcaa ttttagtcaa agtgacatca ccgggaccaa tcatctataa acaagtgcgg
    24661 atcacaaaaa atcaaaagga attttcaatc tataaattcc gctcgatgac cgccacagcc
    24721 gaagcaaaat ctggaccagt tttagctaag agcaatgatg cacgagtaac gcctgtaggt
    24781 aaatttattc gagctgtccg ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc
    24841 gatatgtcga tcgtaggacc acgtcctgaa cgttctttct tcgtcgatca gttcaatgca
    24901 gaaaaccctt attactactt acggcataat gttcgtgctg gtatcactgg gtatgcacaa
    24961 gtctatggaa aatatgtctc tgattacaac agtaaattac gttttgattt actttatatc
    25021 aaaaaatatt cattgatgct tgatttaaag atcttgctgc aaaccatcaa gatcctattt
    25081 gataaaatgt cctctcaagg acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt
    25141 gcttttccga atgaaaaaat ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa
    25201 ttcagcataa tcgtaccagt atacaatgca gaagcaacta ttggtcgaac gattgatatt
    25261 ctaaaagaat tggattatga agagtacgaa gtcgttttag taaatgatgg atcaacagat
    25321 caaacacagg aaatcattca agaactgatt gaaaatgatg aacgttttga attgattact
    25381 acgaccaatc aagggcccgg attagcaaga aacgaaggaa taagacagtc aaaaggagaa
    25441 tatctcttat tcttcgatgc agatgacacg ccagtaaaaa cgatcttgtc agattatgat
    25501 catttgctag aaaagagtcc aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca
    25561 atggacgagg gaagaatcgt ttccgaaaaa gaaaacttag ttacagaatg tcactatcta
    25621 actcacgaag agtttataaa agacatgtat cagttaatga atgaacagtt gatgtatgtt
    25681 gtatggaaca aatgctatcg tagcgatatt ctgaaaaata accaaattct atttaaaact
    25741 tataatagtt gtgaggatcg aatatttaac ttacattatt atgaatactg tcatcaagtg
    25801 atcatgaacc caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat
    25861 caataccgac ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta
    25921 acagacgata ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt
    25981 attttttcta tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag
    26041 cagatattga gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca
    26101 gcaaaaaaaa taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta
    26161 aaagttggaa gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta
    26221 tattagttaa tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt
    26281 ttaggtggag cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat
    26341 caatttgatt ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt
    26401 gaaagattag gaggccgtat ttttgttgta cctaatccta agaaaaatat ttttaaatat
    26461 ttaatagaag tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt
    26521 tattttggcg ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt
    26581 gctcatagcc atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt
    26641 aaatttttaa ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa
    26701 gctggtgaaa gtctgtttca aaaccatccc tttgagatag ttcataatgg tattgattta
    26761 gagctttata gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca
    26821 atgagtactt ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat
    26881 ttgttagaga tttttttaga aatactaaaa atgaattctg attcttactt gattttagta
    26941 ggtgaagggt cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa
    27001 agagtaaaat ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt
    27061 tttgtaatgc cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca
    27121 aaattaaaat tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg
    27181 gattttgtat cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg
    27241 tatgctaata aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa
    27301 atggagagta tatatagtag agatcattaa aaatgttcaa gatattttag gagtgattat
    27361 ttttgaaaat atgttatttg atattggcat acaatgatat tggcaactta aagagattgg
    27421 tcaatcgatt gaacgaaaat gcagttttta ttattcatat agataaaaaa actgatatag
    27481 aaccatttat tgaagcattt aaatcatatg ataatgttga atttttatgt aataaaaacc
    27541 gtgttaaaat ttattggggt ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga
    27601 gagcattaca aaatgagaaa tatttaaaat atgtattatt atctggtgct gattatccaa
    27661 tcaaagataa tgagtatatt tataattatt ttaaaaagaa tagttcagtg gagtttattc
    27721 gtggaataga tctagatcaa attaaacata aagaatttta ttataaacat atagatgtat
    27781 atcaaaaaca tgattatcct agaattaata ggaataacac aactgcattt aaaattttta
    27841 gagctataat taatcgttgt ctgaggatga ttaaacttcc cccaaaaatt agacaccata
    27901 aatttgattt gtatcatggt tctcaatggt gggctttgtc gaaagaatgc ctaacagaat
    27961 taatacaaat gtatgaacaa aatcaacaag actacctgaa ttttaaaata ggtatgtttg
    28021 ccccagatga aaaatttttt catacacttt tttttaattc ctcgttcaaa aataaaaatg
    28081 ttattggagg accagattta ccacttgaat tgaaaaatat tgaagaaact actttacaaa
    28141 cttcgaaatt agcaaatatt catattattg atccttccat gaacaaatgg tttgatgaaa
    28201 gggatataga tcaaataaaa gaaagtgaaa aattatttgt aagaaaagtt aaatcggatt
    28261 actctgataa actattaaat agaattgata aagaaatttt gaaagttgga aatatagatg
    28321 aatagaatag tacctgtttt agagaagagt ttattaagtc gagatgcgtt tcgagtaata
    28381 aatttaatca tttgtctgct ttattttatg cctagtac
//
LOCUS       DACDSR010000026        28306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDSR010000026 DACDSR010000000
VERSION     DACDSR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.026"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWK15_001193"
     tRNA            8..81
                     /locus_tag="IWK15_001193"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWK15_001194"
     tRNA            101..173
                     /locus_tag="IWK15_001194"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWK15_001195"
     tRNA            201..274
                     /locus_tag="IWK15_001195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWK15_001196"
     tRNA            288..361
                     /locus_tag="IWK15_001196"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWK15_001197"
     tRNA            385..474
                     /locus_tag="IWK15_001197"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWK15_001198"
     tRNA            487..560
                     /locus_tag="IWK15_001198"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWK15_001199"
     tRNA            579..654
                     /locus_tag="IWK15_001199"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWK15_001200"
     tRNA            656..726
                     /locus_tag="IWK15_001200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWK15_001201"
     tRNA            740..813
                     /locus_tag="IWK15_001201"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWK15_001202"
     tRNA            838..925
                     /locus_tag="IWK15_001202"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWK15_001203"
     tRNA            947..1018
                     /locus_tag="IWK15_001203"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWK15_001204"
     tRNA            1041..1130
                     /locus_tag="IWK15_001204"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWK15_001205"
     tRNA            1143..1216
                     /locus_tag="IWK15_001205"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWK15_001206"
     tRNA            1235..1307
                     /locus_tag="IWK15_001206"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWK15_001207"
     tRNA            1311..1383
                     /locus_tag="IWK15_001207"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWK15_001208"
     tRNA            1388..1458
                     /locus_tag="IWK15_001208"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWK15_001209"
     tRNA            1471..1544
                     /locus_tag="IWK15_001209"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWK15_001210"
     tRNA            1556..1627
                     /locus_tag="IWK15_001210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWK15_001211"
     CDS             1893..3086
                     /locus_tag="IWK15_001211"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9546810.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWK15_001212"
     CDS             3216..4688
                     /locus_tag="IWK15_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9546811.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWK15_001213"
     CDS             4771..5559
                     /locus_tag="IWK15_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9546812.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWK15_001214"
     CDS             complement(5626..6294)
                     /locus_tag="IWK15_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9546813.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWK15_001215"
     CDS             6409..8121
                     /locus_tag="IWK15_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546814.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IWK15_001216"
     CDS             8124..9893
                     /locus_tag="IWK15_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546815.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IWK15_001217"
     CDS             complement(10098..10664)
                     /locus_tag="IWK15_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9546816.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IWK15_001218"
     CDS             complement(10664..11620)
                     /locus_tag="IWK15_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9546817.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IWK15_001219"
     CDS             complement(11620..12276)
                     /locus_tag="IWK15_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9546818.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IWK15_001220"
     CDS             12646..14664
                     /locus_tag="IWK15_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9546819.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IWK15_001221"
     CDS             15091..17505
                     /locus_tag="IWK15_001221"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9546820.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IWK15_001222"
     CDS             17853..19406
                     /locus_tag="IWK15_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9546821.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IWK15_001223"
     CDS             complement(19787..21571)
                     /locus_tag="IWK15_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546822.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IWK15_001224"
     CDS             complement(21586..23307)
                     /locus_tag="IWK15_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546823.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IWK15_001225"
     CDS             23407..24168
                     /locus_tag="IWK15_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9546824.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="IWK15_001226"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="IWK15_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28306
                     /locus_tag="IWK15_001227"
     CDS             26075..>28306
                     /locus_tag="IWK15_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546825.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8925 a         5059 c         5989 g         8333 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaagtg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggattcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DACDSR010000027        27680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDSR010000027 DACDSR010000000
VERSION     DACDSR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.027"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(205..1149)
                     /locus_tag="IWK15_001228"
     CDS             complement(205..1149)
                     /locus_tag="IWK15_001228"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9546826.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1445..2389)
                     /locus_tag="IWK15_001229"
     CDS             complement(1445..2389)
                     /locus_tag="IWK15_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9546827.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2610..3824
                     /locus_tag="IWK15_001230"
     CDS             2610..3824
                     /locus_tag="IWK15_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9546828.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3846..5861
                     /locus_tag="IWK15_001231"
     CDS             3846..5861
                     /locus_tag="IWK15_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9546829.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5944..7317)
                     /locus_tag="IWK15_001232"
     CDS             complement(5944..7317)
                     /locus_tag="IWK15_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9546830.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7343..7627)
                     /locus_tag="IWK15_001233"
     CDS             complement(7343..7627)
                     /locus_tag="IWK15_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9546831.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7629..9644)
                     /locus_tag="IWK15_001234"
     CDS             complement(7629..9644)
                     /locus_tag="IWK15_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9546832.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9823..10128)
                     /locus_tag="IWK15_001235"
     CDS             complement(9823..10128)
                     /locus_tag="IWK15_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9546833.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10217..11248)
                     /locus_tag="IWK15_001236"
     CDS             complement(10217..11248)
                     /locus_tag="IWK15_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9546834.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11455..12330
                     /locus_tag="IWK15_001237"
     CDS             11455..12330
                     /locus_tag="IWK15_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9546835.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12482..13324)
                     /locus_tag="IWK15_001238"
     CDS             complement(12482..13324)
                     /locus_tag="IWK15_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9546836.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13416..15293)
                     /locus_tag="IWK15_001239"
     CDS             complement(13416..15293)
                     /locus_tag="IWK15_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9546837.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15290..16711)
                     /locus_tag="IWK15_001240"
     CDS             complement(15290..16711)
                     /locus_tag="IWK15_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9546838.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16714..20103)
                     /locus_tag="IWK15_001241"
     CDS             complement(16714..20103)
                     /locus_tag="IWK15_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9546839.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20360..21751
                     /locus_tag="IWK15_001242"
     CDS             20360..21751
                     /locus_tag="IWK15_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9546840.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21824..22471)
                     /locus_tag="IWK15_001243"
     CDS             complement(21824..22471)
                     /locus_tag="IWK15_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9546841.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22479..23165)
                     /locus_tag="IWK15_001244"
     CDS             complement(22479..23165)
                     /locus_tag="IWK15_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546842.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23171..24244)
                     /locus_tag="IWK15_001245"
     CDS             complement(23171..24244)
                     /locus_tag="IWK15_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9546843.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24466..25140
                     /locus_tag="IWK15_001246"
     CDS             24466..25140
                     /locus_tag="IWK15_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9546844.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25145..26485
                     /locus_tag="IWK15_001247"
     CDS             25145..26485
                     /locus_tag="IWK15_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9546845.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26634..27620)
                     /locus_tag="IWK15_001248"
     CDS             complement(26634..27620)
                     /locus_tag="IWK15_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9546846.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8063 a         5240 c         5025 g         9352 t
ORIGIN      
        1 ccacagataa acattgataa cgattcgctt attgtaatca acagattaaa tttcatatgt
       61 tcatatgtac ggatacattt tcggaccgat taattttaac cattctgttt atttgcttac
      121 ctattagata tcaataaaaa actgcttaac agctatttca gctgttaagc agttttagga
      181 tttttagttc atattcattg tttcttataa tacagtgttc aatacttctc ttaaagcact
      241 tgttgatttg ctgaacattg ctttttcttc ttcgttgatt gataattcaa caacttcacg
      301 aacaccattt tcgtcaacga ttgatggcac acctgtaaag atatcttttt cgtcgtattc
      361 accagttaaa taagcagata caggcaatac agcttgttcg ttattcaaga tagcttttac
      421 gatacgtgca gtactcatac caataccata gtaagttgct tttttacgat caatgatctc
      481 ataagcagca ttacggactt tatcagcaat cacatctaat tcatcttggg caatacgatg
      541 gtctttttct acaatttcaa acactggttt accaccgaca gttgtatgtg accatgcagc
      601 aacttctgaa tcaccatgtt cacctaagat atagccgtgg acgctgcgtg ggtcaacttt
      661 taatttcaat gcgatttctt tacggaaacg agttgtatcc aatgttgttc cagtaccgat
      721 aacacgtgat gttggcagac cagattcgtt ccaagcgata tatgtcaaaa tatctactgg
      781 gtttgaagca acaacgataa taccgtcgaa tcctgatccc atgatttctt ttacgatttg
      841 gcgcatgatt gacgcatttg ttttaactaa atctaaacga gattgaccag gtttttggtt
      901 gataccagct gtgataacaa cgatatcagc atctttacat tcttcatagc cacctgacca
      961 tacagccaca ttttcatcgc cccaagccat accatctaaa aggtccaatg cttccccttc
     1021 tgctttttct tgattcacat cgatcaatac taattcatta gagattcctt ggttgatcat
     1081 tgcataagcg atacttgttc caacaaaacc tgtaccgacg attactactt tacgacttgt
     1141 ttttttcatg aataaacagc tccttttcat aactatttgt tactttcatg aaaagtatag
     1201 tcttgtttat tcattttatc aacatgttaa ttataatagt tgttattata atttttgatg
     1261 gaaacgaatt cctgaatacg cataacagat aaatgacaaa tgtttacacc tatatcccct
     1321 gataatacaa ccaaaagttt agaactgaaa ataaatccaa aaaaattcgc tgcaacgata
     1381 taataagaca gcaatcgcac ctaaaacaat tttccttcat atgctttagt gaaagatttt
     1441 cttcttaatg tttatttctc cattcattgg gagtacatgt tgaatacttc ttgaataatt
     1501 tgaataaaga agtcgtttgt ttatatccga tacgattagc gatttcttcc aacgaaaaac
     1561 tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt ctttagaacc agttcttgaa
     1621 aggaagcatc aaaatacatt ttcaatttat tactaagata gaccggattg tagccaaaat
     1681 gagctgctgt ttcttttaac gtacaagaag taaaatgctt atcgatataa tctaatactc
     1741 taagcatctc attttctgcg catccatctg ctgatgaaaa gctttgttgt tctaataaat
     1801 gagtcaattc gatcagtaat agtgatgtat agagatttaa cgaacggatc ttatgactgt
     1861 atgtatcaaa ataaatcaac agcatatttt caatcaaatg cgtcaaacga cttgtttttc
     1921 cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc ttttgatgca tttatcaaaa
     1981 aagaagaaag tagattattt gtgcttgcca aatgattcaa tatctctgta gataaggttt
     2041 cttcttttaa taagatgttg attaaaatat cctcttgccc tgtgtattcg attctttgag
     2101 tagcttcttt atcaatgatc accacgtcac tttcttccat ctctatcctt tctccattaa
     2161 taatttgtaa acatgtaccg ctatacatat aattgatttc aacaaaatca tgtgtatgct
     2221 cgggaacttc cgcatatcga cggtgtttac tgatatgaat atttccctgt tgaaatagat
     2281 cccctcggtg gatttttggc ggtgtctgaa gatggtaagc taagtttaaa tcattgatat
     2341 tcattccttg ttcacgctgc agagtttcga ttgattcttc gttacgcaaa aaaaatgtct
     2401 aattcagcta gttccaattg actccctcct ttttgtaagc ggtttctttc gcttattagt
     2461 tcacgctgta ctgctattat atgcaaagct atttttggtt tcataaagac tttctatggt
     2521 tgggacatga acttcctgtg attccgctat tgagagaata accacttcat actatatttt
     2581 tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt
     2641 ctctgctgtc agtctctctt attttgactt ctgctggtgc agtcacgtct gcaattccca
     2701 gtatgtcagc tacttatgcc acgcgttctt tatctgctgt tgaattatta acgactgctc
     2761 cctcttttat ggtcactatt tttgttttga ttagtagttt tattgctagg aaaatcggac
     2821 aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc ctccccctct
     2881 tctcacaaag ttacagtatt gtcttagctt caagagctgg actaggtatt ggctttggat
     2941 tgatcaattc tttggcagtc agcatgattg ctgcctttta tcacggagat gaacgagcct
     3001 ctatgattgg ttttcaaagt gcatttcaag gatttggtgc agctttaatg acatttgttg
     3061 caggacagct agtacagttc ggttggcaat ataccttttt agtctacgcc atcagcttac
     3121 ctattcttgt cttgtttgtt ttgtttgttc ctacacctga aacaactgaa cttcaatcgc
     3181 atggcacagc cccaacaaga caagtaatca aaacgaatcc taaaatgatt agttatggta
     3241 tttttctctt gctggtcatc gtggtatata atgctgtatc gattaaatta gccacagtcc
     3301 tgacgactta ttctctagga aacgaaacta atgcagcgaa tatcttaaca atcatgcaat
     3361 tggcaagtat ggctgccgga gtttgttttg gctggcttca gacaaaagca catcgctata
     3421 tgcttacact ttctttacta ctgatgggaa tcggctttgt attgattgcc actgtgataa
     3481 acctgtatgt gattgggttt ggtgcgatcc taacaggaat ttctttttcg ttattcattc
     3541 cttacttatt caaccaagta agtatccaag cgcccaagta cgcggagagt ttcaatactt
     3601 caatcttatt ggttggcgct aatttaggta ctttcattgc accatatggt ttgctctttc
     3661 tctcttttgc ttctcttggg agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc
     3721 tgttgattag cgcgcttatt agccttctag tagtacacag gacgaataga aagacagtga
     3781 taagagtaga tgaagaaata aaagaacaat tgacaacaaa ataaatttga tggaggtcgt
     3841 gtaaaatgac aaatgaaaaa cataaagata gatggctttg gtatcctgga gactttgaga
     3901 tccgccatgg actgctacaa aatttccaac gggaagaacg aggttttgac tggccagcgt
     3961 actggtacat ggatgattgc catcggaacg tcaagtttaa gagatattac tttcttgatc
     4021 aaccttccat gtttaaagtg acgatccaag gagttgggta tgtagaaatt aatggacaaa
     4081 aacatccatg tggaaaatgg ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg
     4141 taggacatac atcagggcta ccagcgatgt ttatcgaggg agacgaagta aaatcagata
     4201 ttggctggac agcaagtaat tttattgaag aatatccagc tggctggagt ccgctttatg
     4261 tcgatatcgc aaaagatcca aatcaaatct attatcaaaa agaatgcgtc catccagtca
     4321 ctgaaacgga gattggcgga ggaatcttat tcgattttgg gcgagcagtg aatggctgtg
     4381 tagaactaat atctctggat gaccatatcg aatctatcac tctatgttat ggagagtccg
     4441 atgtagaagc tatggatatc gattattgct actataagca aataggaatg acaagaaaaa
     4501 tgaaagctcg taaacgcgct tttcgctatt tgtttatacc agaggtttct actggaatgg
     4561 ttcaacttca agtttttcat gaatttttat ctctttcgaa acaagctgct ttttcaagcg
     4621 attcttcttt actgaatcag atctggtcag tatcagaaga agccttcaag ttatgtagtg
     4681 atttgttttt tatcgacggt atcaaacgag atcgctggat ttggtctgga gatgcgtatc
     4741 aatcattcat gatcaatcaa tatctttttt ttgacgagga aatcaataaa cgaacgatat
     4801 tagcattaag aggacaaaat gaaatcaagc agcatatgaa tactatcgtt gactattcga
     4861 tcttgtggtt gattggtatc gaaaaccact atatgatgac gaaagactat gaatttttac
     4921 gcatcgtcta ccctaagatg caatcattaa tgagatactt gatggagcaa acgaatgaat
     4981 tagggtttat ttatgggaga gcaaaagatt ggatatttat tgattggtct gaaatggata
     5041 aagaaggaac gcttgctgca gaacaagtct tattactgaa agcctatcat tccatgatca
     5101 cttgcggaga tatcttagct gaaacatatg gagagaacga aaattatcat actcatctct
     5161 atcaagaaaa atatcaacta ttgaaacaaa acctatttga ctacttttgg gatgaagaac
     5221 ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata
     5281 ttttcgctgt tttattcgat tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg
     5341 tcttattgaa tgacactatc acacagatta ctactccata tttcaaattc tatgaacaag
     5401 atgctttatg caaactcgga cagacgaata tcgtctacca aacgattctt gattattggg
     5461 gagggatgtt atctcatgat gcggtaactt tctgggaaga atacgaccct actcaaacag
     5521 gaaatgaaaa gtacagtatg tatggagatc cttatggaaa aagcttatgc catgcatggg
     5581 gagcaagccc aatctatctt ttaggacgct attttatcgg tttgtatcca acaaagccag
     5641 gttatgaaga atttatgata caacctaatc tagattcatt tgaaacactg aattgtacgc
     5701 tacctatcaa agatggaaag atttcgcttg aaaaaacaaa agataagatc gtgattactt
     5761 ctgatagaac aggcggaaca gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac
     5821 agccacttgt cttaacaagc tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga
     5881 catatcagaa gactttaaat cttcttctga tattgtccaa gcaattttta ttttgaagtt
     5941 tgcttaattc tgttctttag aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga
     6001 taatacgaca agtaaaaaga aagcgatgat tccaactgtc aaggctattt gaccgtattc
     6061 tcctagtttc ccaagtacaa gtcctggtac tagataatca gtatcggcaa atgtagaaga
     6121 agcgatttta agatctccta atactggaag gataaataat ggcagccaac taatcaataa
     6181 gctattgaaa gcagaaccta ataccgcccc tcttctaccg ccagtcgagt tgccgaagac
     6241 accagaagca gctccgcaga agaaatgtcc tactacccca ggaatgataa ctgtagtttg
     6301 caagcctatc atagcaaaca tggatagtgt tcctacaaag aagctaacaa aaaagccaat
     6361 taatacagca ttaggtgcat atggaaaaac gataggaaca tctaatgctg gtttagaatc
     6421 gggtacgagt tttttagcaa tcccttggaa tgctggaacg atttctgcca acaccattcg
     6481 aaccccttga aggacgataa caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa
     6541 gattacagcg ttgtctccat tgcttagttc tctttcaaca aatccaggac cagctaacaa
     6601 agcaagtgcg acatatacga tcgacatcaa gagcatgatc gatacagtag aatctcgcaa
     6661 aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa
     6721 ctttccgatc catccactag aagcataacc gatatttccg gtatgcccta acgcaacttg
     6781 attgtttcca gtgatgttct tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag
     6841 tgttccttcg aagaccccgc ctaataagat catccacaaa tttgcagtaa accccgcgct
     6901 tactaaaatg actgctgtta gacaagaaac ataaagcatt gcttgacctg taagaaaaat
     6961 gaatttatat cttgtgaatc tagcaagtaa aatattcacg atcatcccta ctagcatgat
     7021 caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc actactgctt cattgctagg
     7081 aactactcct tgagtatgca gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt
     7141 actgattaaa ccagcaccgc cgatcagtac taggaatccg acaaatgttt tgatagttcc
     7201 ttggatcact tctgaaaatt tcttcttctg aagaacgaga ccaagcagtg caatcaaaga
     7261 aacgaggatg gagggctgac tgacgattga cacaaacaaa ttcaacaatt tatccattga
     7321 tagttcctcc cttgaatcat ttttattctt ttgacaagat cttcgttact ttttctttta
     7381 cttcttcgct gtcaatgatc gattgcaaag gtacgagctt ttcatcaggt atagaagtta
     7441 gcgtatcttt caaagaatgc tcaatgaaga aataatcagc catatcgcta gatacacttc
     7501 caacatccgt atgacttact tcaaactcgt ccacatctgc tccaagatct gcaagtgctt
     7561 gtttgatgtt catctctacc ataaaacttg ttcctaatcc actttgacat actgcaaaaa
     7621 ttttcatatc attcgtctcc ttttcttatg atcgctaata tttcttcttt tgttgatgcg
     7681 tgtaataaat cttgaagatt ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc
     7741 cgaagatgtg cctcattatc gatagcagct aaacagatga acaaactaat gggatgggct
     7801 ggattatcaa gcagatccac aggttgttga agtttcagca aagacatccc tacttcgttt
     7861 acgccatctt ctggacgagc atgaggtaat gcgatgtgat cgccaatatg gataaacgca
     7921 ccatactgct ccacgcgctc gatcatagct ttgatataac tttcttctat tttattttct
     7981 gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc agttttttgg ttgatccgat
     8041 aattggatca tttcttctgt taataattca gatagcatag gacgttgatc ctccttcttt
     8101 tctaaaagtt tgttcatttt acgatttaat acttgataga gcttttcttc gtctacgcct
     8161 tcttttaatt cgatataagg cagtagcgcc gacaacaatt cttgggcaga aggcattgaa
     8221 aatcctggta tcagccaatc tctttgtact tgattgatca gctgattttt ttccaagtca
     8281 gtcatgattg gatgcagaac atagagagac tttttcttat cagtaagtgt gagaggtaca
     8341 gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt gtaagcggtc agctgaatta
     8401 gtctctgtaa aaaagatcat cgggaataat tgctgaagtt cactttttaa gatcaatgaa
     8461 gagctaatgc cacttggaca gacgatcacg gctcttattt ttctatttct ttcttctgcg
     8521 tccgcttttc tgatctctcc gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg
     8581 atgctcttcc ctattcgtct ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg
     8641 gaagcatatt gatgcttaat ttgatcgatc atcacattag gtaaataaaa agaataggaa
     8701 atacgaaaat aggcagggac caagtgataa aagacattca tcaataattc acgaaacgaa
     8761 gcaaattcta cggccatcag gaattccatg cgatggatga cttctgaaga acattccagc
     8821 aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga tcgatgctaa gcaaagagag
     8881 aaaaatataa tctccgaatt ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga
     8941 ctaatgtcag tgactgcaag agaagccaac agttcagcat cttttaccag ttccatggat
     9001 gtttttccaa ttcgaatggt tgtcaaactc aaataataag ctaattcatc cattcgattt
     9061 ggtgcgaatc gaagatctga tagtgaaatc gttttatcta gcaaatctct aactttggcg
     9121 taataatcaa tggaaagggt tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac
     9181 tgcatcaaac gagcagtgcg attttttgct tctcttctta atattgcttc tgaccctagc
     9241 agcctaaaac cagttttcct agtatacgac agctgacaat cttccacttc taattcattt
     9301 cttatctttt tgatatctga caggactgtc cctttagaaa cctgaagaaa atcttgaaaa
     9361 tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca aataaagaag atctcttcgc
     9421 tcttgttcgg tatacacagc ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg
     9481 attgctcgga atttctttat acggatacgg gtatcatcca catattcggc aaaaggagtt
     9541 aattgtttga tcgcctcgct tgtatttact tcgatgatct ctgctgcttt ttctagactg
     9601 atttctcttg gtgttttcag gagataacta attttgtagt tcacagcgct gttcacctcc
     9661 tacttatatt ctagtatggt agcgctttta aatcgagggg tgtttggtac aacttacagt
     9721 tggatttttt ttacgactgt accgaacgat ttctatcgaa tggatacaga aaaaaagcag
     9781 gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc gatcaatgat gatcatgttt
     9841 attttttgag gtcagttctt caaatttttc tttgacatct tcaaccgctt tttcgatatc
     9901 tttttcaaag tgattgggct cattaggctt tgtttcctct tctacagaat ttgaaccaga
     9961 taaagctgtt tctttatttt ctgtttcacc agagtttgtg ccagctaatg attcttcttt
    10021 gtcttctgtt tcaccagaat tagttcctgc taaagccact tcttcttctg ttttaggggc
    10081 cattgaacct cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt tggttcctcc
    10141 tagtagttct atactttttc atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc
    10201 gattcttaca aatatgtcat atttttcctt ctgattcttg aatgcttttg attccttctt
    10261 ccaaaatttt catttgtaag attgtttctt catctttgat gactttttct gcgccattaa
    10321 ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc accaacctcg gaaattactt
    10381 tttcttcatg ataactccct tgttcatcta tataggtcaa tacaccataa tgttcgggca
    10441 aatcgatacc aaagtcttca tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc
    10501 gatcttttgt ttgtttcacg aacatccctt tttttccgta aacgacgaaa cttggacgtt
    10561 ctttgatgcg gaaataactg gattttatcg aaattttcat atttccatag aaaaaatcca
    10621 gatcgaagta atcattcatt cgtcctttcc ctaaaagttg acgtacatca tagctgatat
    10681 catctggttc gccgaaataa gaaagaactt gatctactgt atgacaagca tgtccatata
    10741 aatagctgtt cgcagtagaa aattcatgcg tattttctgg tacttccggt ctgaaataat
    10801 cataatgcat ttctacttct aaaatatccc ctaattttcc gctttcgatt actttttgga
    10861 ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca ttggataaat agaccttttt
    10921 cttttgctaa tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta
    10981 ctagtacatt cttaccatgt tcgagtgcca tttttccata ttcataatga aattgactag
    11041 gtgtagtgat gacaactaat tggatctcag gatcatccca aagatcagac agctgatcag
    11101 tatattcgat gtcttcgaat gttttccaca ctggttcaga tggggaaaaa atttttttga
    11161 tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt gtactctttc
    11221 cattgccaag ataagcgatt gtaagcatga gtggtgcctc cttttattga tgttttcagt
    11281 atatcctaga aaagcgcaga aagtatttat aatagctatt ctggtaaaaa agttcagaaa
    11341 aacacttgtt atcattcggt ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa
    11401 agatagtagc ggaaccaagt gtttatcagt aagatagaat ttggaggtca gatcatgctt
    11461 attgaagaaa aactaacaaa acaagagttg tttaccacga cagagaaacg gattgtcgat
    11521 tacatccgta ggaatatcga agctgcagtc tacatgacga ttgaagaatt agcaaaagct
    11581 acgtatactt cccattcagc tatcattcgt ttatgcaaaa aaatgggatt cagcggattt
    11641 aaagaattcc gtttcgagct ggggcgggaa gttcatcaat tgattgctca gttcaatcaa
    11701 acgattgatc ctaattttcc tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa
    11761 atggcggatt tatcgatcca gtcaataaaa aaagcacaat tgcagattga acatgaagat
    11821 ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa tctttctttt tgcaaaaggt
    11881 gattctcaaa tcacggcaag gaaattccag aataaaatgg tcaagttgaa taagtttttg
    11941 atcatggctg aagaatacag tgattcttct tggaatgcag ccaatttgac tagcgaggat
    12001 tgcgccattt ttattagcta cagcggtcgc atccatcatt atgaacgaat aatgacctat
    12061 ttgaagcatg tagaggcccc taccttgctg attaccggaa atcagcattc agaaatggcc
    12121 aaacaggctg atatgtgtct tgttatatcg caggaggaat atgattttgc taaagtagcg
    12181 actttctcct ctcaaattgc cttcgattac gtattaaata cgttattctc agtgatatat
    12241 tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa
    12301 ggccttctta aagaatatcg gaaaacttaa gaaaagatcc tcatttagta ataagaaaga
    12361 tagtgtttga taggctctct ttgatatcat cataaaaaca ggctgaagtt ttctttttca
    12421 aacctcagcc tgtttgttta cggaacgatt cgaatagata tattatagaa atgaggaata
    12481 gctaacttct gtttcttatt ttgaacgatt gacgaaaaag atatataaga aggaggaaaa
    12541 tcagtataca tgcagctaac aatccataca ggaaaaatcg tttgttctcg gatactttct
    12601 gtacttcttt ttgaacttct tttgcatgat aaggaacccg gtgccctctg acaagtaagc
    12661 gatgactgtt gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt
    12721 cgattctcag atcttttgta tcagtcggtt ctaccgtctt gatctgatcg acttgataag
    12781 cgagtgtccg tccattgatt tcgatataga aagaatcacc taaagtcagt ttcggtaagt
    12841 ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga aataacggca tgtgtatttt
    12901 ctccccccgt aggataagac gttccttcca ataatgaggc acctttttct agcagcacag
    12961 tattcgtttc atcaaaaatt ggcaaatgga cattgatctc agggatcgtc aaaataccga
    13021 tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg ttcttttttc ttagtaaagg
    13081 gatctgcacc tggatttcca ccttcttttg ctaattgctg gtttttcttt gtcatgtttt
    13141 cttggatttt agccatgact tcttcatttt ctttgaccgc ttgttgctga taatgagaga
    13201 tgatctgctg atccagataa ttattcagcg cgtcgctaac aaatggataa gctagtatcc
    13261 ctattcctga gagaagcaag aaaagcatca gcagatctag aagtttcctt ttctttggtt
    13321 tcatttgtgt tctccttgtg atgactgttt gataataaaa gaaaaaggag catttgcgta
    13381 atccgctgct cctttttcca attcttgcaa aaagactaaa tctgactgtg cttgcgtcta
    13441 gcaaagtaca gtccagcaat cagtagaagg actactcctg caccgatata cacatagatt
    13501 cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa
    13561 atcaactgtc ctgctgttac ataagattgt tcatcgatag taaatgctac acggtttgtt
    13621 aatggaacat atttttctgg cgctttcgtt tcttccagat agtacgtgcc atattttaga
    13681 cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc
    13741 gatacccagc tgacggcttt tgtggatggg tcgatcgata aatatttcgc tgtgtcacta
    13801 tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca
    13861 tctactttaa caaatcgttt gcccccagta acgacatcga ctgacggtgg tgtttgatca
    13921 tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc ctttggttgg atctgctttt
    13981 tcgttcaaat gcatatagta aacgaatttc aatgtaccgc caggagttaa tgaaggaata
    14041 taattcggat caactgaaac cgtgaatccg tctgtttgct cagtgacaga ataatttact
    14101 gggtcgattt ctttatttcc atcatataag gcataagcat acgttcctga agaatcatta
    14161 tcaaatgtta aagcagcgtc atgagtatcg ataagtttga atgttgtgta cttgttttgc
    14221 gttccttctt tatcagcgat acctaatggg atattgacag aaatctcata ttgaattttt
    14281 tcaccgattg cgacatcttt tccattcaat tgaggtgttg ttttatcaac tttagacgta
    14341 tcatttttga tggtcttttc tactggtgtt tggctatttg ccaggatctc aaaaggcgtg
    14401 attgtttgct tttcaatcat ttccgcatta tcaggagctt ttacttcttc taaattatat
    14461 tttccaactt ctaaatgatt aacgatcaac tggccttttt caatagatgc ctcggcaaag
    14521 tcattgttgc cgatgtcata agaatgacca gtaatgaaat gtttggcttt ggtttgatca
    14581 gttgtccaag tgtacaatcc atctgtgaca ctttggatgt attttttgac gcttggtgtt
    14641 ccttcttctt tagaaataat aaattctgct ccatttagtg cttcgttttc ggcagtaccg
    14701 atttttgtaa ctttcaacgt tccatcatta ccgactgtat ttttagggta gagatggata
    14761 gtatctagtt cttctgtccc gtatttataa gagccatctg cttgtttgat catctcatat
    14821 acagggaaag ctaaaaccat gtttgcggca gctgacactc cgtcttttgg ttcttctttg
    14881 atcgtgtaga ctgcatcttt cccattttgt tttttaggta aagataaagt gacatttcca
    14941 tcagcatctg tcgttcctga agcaactggt gtaccaggag tcaaagactg gactgcttgt
    15001 tttgctgcat ccacggacgc tcctttatct cgttgcgcat aaaattcttg agtgacgtta
    15061 taaactgaaa atgaaatatc ggctaatcct tggtattgat cgaattcgct catttctttc
    15121 ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt tctttttatg aaggatgact
    15181 tgagctgctg tctcctctgc aaacactttt tttgcgccga agctgtttgt gagtaatggt
    15241 aaaataagga aaaggactcc taacataacg tttatttttt tatgattttt cattttttca
    15301 ctcctgttct ttttttgata aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa
    15361 cgcctaaaag tgtatttttc gtttcgtttg tttttggaag aaatggacgg ctattttctt
    15421 ttttattgac tactttcaat atactgtccg cctctttatc ctcagaaaga aaagttgaaa
    15481 tggtaaacgg aatttctgtt tcacttaaaa tataaccaga aggtgcttcg atctctttta
    15541 atcgataact tcctgttttc aacccggaaa ttgaaaaagc gccattttta tcggaaatca
    15601 ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc gttggctgtt tgctggaggt
    15661 aatttcctgc tttgttttta ataaggaata ctgcgccagc taatcgttta tctggtttat
    15721 ttgtctcgat tttgacaaat tgcttactcc ctgtatatac agagactttc tttgtactga
    15781 caccaaaacc aaaatccagt gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac
    15841 tgcttagctg catctgataa gaaatggtca gtttttttcc agcatatttt tccaacgaag
    15901 ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg tatgacaaag ccttctgtca
    15961 gcttttctcc attcgatgaa atcgttaaac tattaggtaa aaacgttaat gcttcatccg
    16021 cactatctga caattcgaac ttttgatagt caaggatatt tacaggaatt gtcgtatgca
    16081 aggaataagt gattttttca ccaatcgtaa aatcatttct aggctcttct aataccttct
    16141 caaaaggtgg atcagggtag tcgttttctt catttttagg atacaaatgg atagttgaaa
    16201 gtttttgatt gttttgtcca tgtacaggaa gaacaactac catattctct gccttttctt
    16261 tgacgacttc tggtactttg gattcaatga aaagatacgc tttatctctt ttttcttgat
    16321 ctttagcggc cagagaaaaa gaagcgatac catcttcgtt gttaagagtt gtagttgttg
    16381 cttctgcggt aaacataccg gaagacgcac cgttttttgc gatctctgct tgtgcttctt
    16441 ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc tgtgacatca taaacttgga
    16501 atgtgacacc atttaatcct cgatacgttt gtaataaagc tttttcttgt ccgtcatttg
    16561 gatgattttt cggcagttgc ccgtttggga aaagcaattt gtgtaaagta atctgaacca
    16621 tttctgtttc tgtttctgtt gctacctgag taaaagctgg tttaaatagt aacaagaaga
    16681 gacacatact aagccaacca agttttttca tatttagctg ccccctcttt cttttttcaa
    16741 aaaaagatac caaatagaaa acgcacaacc aatcatccct actagataga ttcctaaacg
    16801 gccaattcct cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat
    16861 ctggttgttt ttggctcctg gtgacagaac agattcatga tcttgtccat ccacttgaat
    16921 tgacccatct tcgtgtataa ctatagtaac tggctctttt agaccttggt atccttctgg
    16981 tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa ttttcgaata gaaaggtatc
    17041 tgtttcttgt ccatcttttg gcgattctaa gtccatttct ggtccctgca gtctgaattt
    17101 tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt accgttagat caaacgcttt
    17161 tagtcgattt tgatgagtga gcgtccatac attatttgtc atctcggtat tttttcccgt
    17221 gtaaatgatt tttccatatt tatcgatttg gaattcataa tttcccggcg aagtatcata
    17281 tccggcagga ccagctgttt caacgactcg atatttacca ggtgtgagtg aatcaaaact
    17341 ggcaagacct ttttcattcg ttgtttggct gtcaattggc tgataagtac catttgcttc
    17401 ttttttctgt agggaaaatg ctgctccttt aagatttatc tctttgtccg tcccttgcat
    17461 cgcatactta cgtactgcta caggaacttc aacaaaagga ttttcaatag tcaactggat
    17521 cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt accgtaccat cagaagcgat
    17581 cttgatttcc caagtagtct ttttgcttaa tccatgccct tctggagctt ttgtttctgt
    17641 cagcgtatag gtcatttctt tttgcaattt gacattttct ggaagagaat aggttccgtc
    17701 gccatgatct gttagtaaag tatcaataga atctcctgtt aatttgaaaa cagcgccaat
    17761 aagatccttt tcacctgtag aggaattttt cgttattttc aagtttaacg gtgtgaattg
    17821 tttagtattt ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa
    17881 ttctttgatt gtttggtaac tgaatgcttg accttgatta ttgtattgag gtagtgataa
    17941 tatctcttga taactttctc cgctatttgc agataatttg tcaatgtctg cacgttccca
    18001 cgtatttgtt gtatctttag ctggtttaat gattcgaata tatccgtttt tccaagctgc
    18061 agcatttgtc gtatgttccc gttgaatctc aaaagtaact tgatctggac gatcagctag
    18121 attgttgtca aattcttccc ataacttttt gatgtgaaga ctgactccgg gagctttagc
    18181 agatggtata ccgaattcag ctaattcatt ggtatcaatg tttggctgga aagttgtcct
    18241 gccgttcatt tgataccaga aatttggatg gaagtcctca ttttctgttt ggattctcac
    18301 ttggtaatgg atttggattt cttggtcttt tcctaaatag atctgattgc tcttgatggt
    18361 atttccttct atggaaatag atggagtgac cgttgtagga cttgtcccca cactcttgac
    18421 tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt ggatcattta tttgtccatt
    18481 gcttacagtt gctgagatct gtacagcttt tttggctagg tattcagaga tatctgttgc
    18541 atgatcagct gattcatagt aaagatcgcc tttttcatct gatgaaacca tttgtcgcat
    18601 acgagactct acttctgctt ttgagagacc agctgccgga tcgctttgaa gttggatgcc
    18661 aagaccatgt atttcgattc ctcgttcttt gagtgccatc gcttctccga tggttgcgat
    18721 aaacgtactg tcgattcttc tggatagatt gttttggtca ggtgcatcat agattcttga
    18781 gattagagaa gtatttcccg gccgatcttg cgtattagaa aactgagttc cgtaataatt
    18841 gctgcttgat tgtgcgtgta cccgctgtac tttataggaa aatgttggta caccatccgt
    18901 cagcaaaacg atcacttttt tatgtccatt tggaacggat agcatgcttc ctgcatctct
    18961 tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg attttacttg
    19021 atttttcact gaatcaaatg aacccatctg tactgcaccg ttactgtagc tatatccttc
    19081 gcttgagtag ccgacatatc ccatattgat tttgtctgtg ataccgctat ctgctaaagt
    19141 atcgacaaaa cgatcgacac caatctttac ttcaccgatc cgattattgt cgttcatact
    19201 tcctgaccag tctacgacca atacgagatc aagaggcgtg atatcttttt gtacatttcc
    19261 acgagcattc aaataaacat caaacaatcc gggtgtactt gtttcctttg catactttcg
    19321 tatcgcatag tctgcttgat taccggttcc tccatattcg atatacgaat gggtccgatc
    19381 attaggatct ccattccaag aatttatacc atcccattga tttgttccgt ttttattgcc
    19441 ttgatgatca agaacatttg tatttcctgt aggttgccaa ctggctttag gataagtgcc
    19501 ctgatcatct gttgtatatt ctgtaggaat cgtttgatta gatggcacac tcacagctgg
    19561 caaggataat gtaggcaatg taaatgggct gacagaattt tcgttttctt ttgatgattc
    19621 ttcttctatt tcgtctggct ttggtactga cacatctgta tctttcttat tttctgtctc
    19681 atcaataatg tcatattctt tattatcgac gatcagcttg tttgcctggc tgtggtaaag
    19741 tgctggatcg attggaaaac tcagatcgat agtttgttta gtgtttgatg agatttccaa
    19801 cgtaattttc ccgctattta cctgataagc atagccatct ccttcttttc tttccacatc
    19861 acttaagtaa ttttcgacta gaccaatttc tttttctatc ttttcagcac tggtgttatt
    19921 cgcatttagc ttgatgttca gtgctttttt tccagactga tccgaaatga cttcagctga
    19981 aattgtttct aaaactgttg gatcatctgt agtgtctgct attgcagata atggcacaaa
    20041 gtttgataaa ctcaatatta tcagcatgaa aataactttc agtttcttcc ctgtggtcgt
    20101 catttttccc ctcctttttc tttttttctg ttttgacgtg aatagtgaaa attattaaaa
    20161 taaacaggcg ttacataatt ttatttattt taatatgata attattatac gaagggcgct
    20221 aaagggaatc acaccaattt tcataccaat tggtttcatt tatgaagttg attccttcga
    20281 ttaatgagcg tttcattgaa agaaaagggt tgagggagaa aaacatatga tcaggttgtt
    20341 ggaatgaaac tatagaaaga tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg
    20401 gatgactaaa aaagaacttg ccaaacgaat tggtagttca gaatctacat ttgtaaggta
    20461 tatcgaagag atcaaaatcc gctggggaac ggatgtaacg attcaatctt cacaaaaatt
    20521 ggggtatcgt cttgaacatt tgaatgtacc agcttattta aatgttctga tggaaatgtc
    20581 gcaaagttct acaaacagtc gtttattaaa tgagattatt gtcaatccgg gtaaaacgat
    20641 tgagtattat tgtgaaaaaa tttccatcag ccggtcttct tttacacgta agttgaaaca
    20701 atgcaatcaa attttagaaa gctatttatt aaaagtgatc gttgaccaag gttttcagtt
    20761 aactagcctt aaaagtgagc tccagttgcg tatctttgtt acctttttct ttttaagtta
    20821 ctacggaaga cgcacgttgc catttccaat cgaaaaaaag aagatcaaag aaattatggt
    20881 caagaataat tgtcagatga atttgtttca ggaagaaagc ccatatgagc aatctttctt
    20941 tattatgtat tttgttgtgc atctcatgag agaatcccaa ggaactccat ttgaatggaa
    21001 agctggagac tcttatgtac agttgataaa tatcgataaa aaagacttct taatcttgaa
    21061 aaatctctta ggaaaattga agtacagcag ctatataaaa gtagtgaact attttgtcaa
    21121 tacgattttt tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa
    21181 aaatatgtcc tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt
    21241 tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg atacgactca
    21301 tttgacgatc cgtgcaactt actttgcaca gcagatgcaa caccgccaga tacgtctata
    21361 tgctggattt cgtgcagaac ttcaatttta ttccactcta ctgaatcaga atctgattta
    21421 ttatttatct agctatatct tttggatgct tatcggtaat cctgaaattc accattatac
    21481 ttctgataaa aaaatactga ttatcagcga cttaagtctt cgacatagtc aatatattga
    21541 agaatatatc agtgatattc ttgctgtgca taaaattcac tctgagacaa ctgctattac
    21601 tgaagatcag ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta cgaatcaacc
    21661 cattttgaat gcccatgttc ctcatatatt gattgatgat agtgtgtcat tcgcaaatga
    21721 agaagaactc atccgcttat tcgagttatg aataatcaaa aaacagctat gtataaacca
    21781 ccctgataaa aatttcagga caaatacaca tagctgtttc tttttattgt attccttcag
    21841 caatccaacg agatttatag cgaaagtctt ttaacaactc ttcttttgtc caattatttg
    21901 caatgagatc cgtcaaagtg ttcattacga gggtcatgga aaagtatagg aaactaactg
    21961 cttcttccga cgattgaatg gagaaatgat tgattcttaa taattccagc catttttcgt
    22021 tcattggaga atcagccgat agttctctac gtttggttgt ctgctgatca tttcgctggg
    22081 ttaacaagta taacctttgc caatagatac tttcgtgatc taagtcgcag caccactcta
    22141 aatatttttc gatccccaaa aagaaatgtt gtttgttttc gctaatgaat tttaagatat
    22201 cttgatggac tatgtgagaa ctgcttttga tcgtatacaa ataagcatct tccaaatctt
    22261 gaaaatattt ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt tctgagactt
    22321 tgacttggct gatgtgctgt gagcaaaata tctccagtaa taggtcatcg attctctgtt
    22381 tctttgcgtt cgataaattg aaaaaagttt cttttggcat ccacatctct cctttatctc
    22441 tgattatacg tggatagccc ttcattgtca tgaaggattc attccttctt tttctcttct
    22501 tctgtcaagt acccatcttc cattcgataa acgcgatcac tccattgaat catacggaca
    22561 tcatgagtaa ccataatagt cgctttttgt ttctcatgag cttctttaac tagtaatttg
    22621 acgacttcat atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc taagatcaag
    22681 cttggctcat tatataaagc gcgagcaatt gccactctct gtctttctcc accagaaagg
    22741 tctttaggat aactattttt aagtgactgg atatctaaag attgcagcaa ggaatcgatt
    22801 tttgttgaat cttctttttt tgaagtaact tggtcaacta gttcaaattg ttcctttacc
    22861 gttaaaaaag gaatcaaatt agagctttgg agaataaatc ctatttcttt aaagcgtaaa
    22921 tctgctcgtt ttttttctgg aagctcagtt agtgagtgac cgttgatcac tatttctccg
    22981 gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa
    23041 ggaccaatga tgctgacgaa ttctccttcc tttacataaa aatcaattgc tttcaaagct
    23101 gagacctttg tatgaccaga accaaatgtt ttggtaactt ttttcatcgt taatattttt
    23161 ttcattttta ttaacctcct attgctgtaa ttgggtctac tttggttatc gtacgtatcg
    23221 aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc actatatagc aaccattgtg
    23281 accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca
    23341 aaagagcaag cagaacgcct accaccccaa caataaagga ctgtgctact aaagatttag
    23401 cgatgaagaa atttttgatc ccttgtgctt tcattactcc aaaaatagca gtcttctgaa
    23461 gtgtcatcac gtacatgaaa ataccgacca cggcagcaac cactaagaac aaaaagtata
    23521 tcatcgcatt taaggtcata ttttgggcag aatagccagg aatactttca atgaatgtcc
    23581 caatagttaa taactgtaga cccttcgatg ctttttcttt cgaaatttta gctgaatcct
    23641 ttattacgat cccattgatc ggctgttctt cttcagaagc aaacggctgt tgtccatact
    23701 tcaaagcagt ccacgtatct agggacgcat agatgacggg agaaactgtg taataagttt
    23761 caggaaagat accgacaatc gtcaaaggat cgtcgtaact gccaattttg atcgtatctc
    23821 caattttata gcctgaatca gccagatttt gagagatgat gatttcattc tttgttttaa
    23881 attcatgccc tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg
    23941 taacattttc tttgtttttt cctttgattg ctccgctgta caatccgatt ggtgctttct
    24001 ctcctccttc aatgtatttc agatctcctc ttgttaattg tgaagctgca aatacttgat
    24061 ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc atcgattgct tttttaaact
    24121 cttctgaaag tccattcgct agtcctgaca acatgaatac gacatacgca atcaagaaca
    24181 taataccgac gatcaagcca tatcgtaatt tagaatagcg aatttctttg atagctagaa
    24241 acatttgttt ttcctcccta tgatagattg atagtgacaa aatgtcactt gttgaatagt
    24301 gtaactctaa agtgacaatt tgtcacttgt gttgtttttt tcttatcaat aaattgtcta
    24361 agacatcttt cgataaaacc ttaacatgag tcataaaaaa tttatttaag ccttatttat
    24421 gctttccatg ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat taaactatta
    24481 gtagtagaag acgataaagt attatccaat aatataaaag aaatactcgc tgcaatcggc
    24541 gagataacac aagtttatga tggtagtgaa ggactgttcg agttggaaag tggagtttat
    24601 gatcttgcaa tcttggattt gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa
    24661 atacggaaag aaaaaataca gactccagta ttgattttga ctgcaaaaga tagtttggaa
    24721 gataagattg aaggatttga acgaggtgca gatgattatc taacaaaacc tttctatcga
    24781 gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa
    24841 aatgttctga agtataagga tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa
    24901 gaacaagttt tgcccataca aggaaaagag tttgaattgc tcgtttattt tttacaaaat
    24961 aagggaatca ttttgacaaa agagcagata tttgaccgga tctggggatt tgattctgat
    25021 acgacgatta cagttattga agtttatatg agcaacttac gaaaacatct gcgaagtact
    25081 ggattggata aagaaatcaa aaccttacgt aatgtcggtt atatactgca aagggtgtga
    25141 tgcaatgaat ggagatatta cacgaaagca acgtagtcgc ttttttgcga caaatgtagc
    25201 agcatttgct tttatctttt ttcttttggg attcattact ttacagatat taaaccaatc
    25261 tgcatacaga gaaacagatc tgtcattaga gaaaataaat tttgattctc gaacagtaca
    25321 attagagata caaagatatc agcaaggcga tcccttttta aacccgactc aaacggaaca
    25381 aaacagaaca aacatggaac agccattaat ggaaccatca aatgaccgtt ttaacactca
    25441 agtgatcttg tggtctaaaa atggtgagat attgaataaa gcagctattg gtgggcgttt
    25501 caaccaaatc aaagaattaa aaatggatac gaaaaatctg aataccatcc aaaatattga
    25561 attaaatgaa gacagtggct catctgccct ctctttccgt tcgattacga aaaaagcgga
    25621 gacgaatcaa gatggcgttg cctacatcca aattttagaa aatacaaatc aaatcaagaa
    25681 ttcattagcg acttttcgca cgattatgat tttgtgtatg attgtttttt ggattctctc
    25741 tattggtatc agttattact tgtctagtct aagcatgcgc cctattattc tttcttggag
    25801 aagacaaaaa gaatttgttg agaatgcttc tcatgaattg cgcacaccgt taacgattat
    25861 tcaaaatagt ttggaacatc tttttaccaa gcctaatcat actattattg aagaatccga
    25921 aagcatcgct caagcattgt cagagacgag gcgcctcact ggattgactt cggatttgct
    25981 gacaatcgct cggaatgatt ctgatcagca gctgctttca aagcaaatga tcaatacaca
    26041 agaatatatc gaacaacttg ttaaaccatt tcaagagatg gccatcatag atggaaaaga
    26101 gtttattctc cataacgatg caacattaga agtaccaatt gatgaaaaaa aaatccatca
    26161 agttttggtt attttgctgg ataatgcatt aaaatatacg aatccagaag ataagatcat
    26221 tttggattca tctgttaacc ataaagaatg gctgattcac gtaaagaata ctggcccttc
    26281 tatcagtgaa gaagatcaag caaggatttt tgatcgattt tatcgggaag atcaatctcg
    26341 ggcgaaagag actggcggat acgggcttgg cttggcaatt gcaaaacagg taatcgaaca
    26401 gcatcatgga cagatttctg tatccaatta tcttccacaa ggagtcgatt ttaaagtcca
    26461 attgccgttg aagcctaaaa aataatgctt atccctaaaa aagggctgtg acaaaaatct
    26521 tgtcacagcc cttttttgtt gcgaataaac ggtgttccag aagtagcttc ttcggaaata
    26581 agccgaaatt tctttaagct gaccacttct gtcacaacct ctttagctta cgcttacgat
    26641 tttttattgc ggtcttcaat gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg
    26701 ctaacatctt ctgtcacttt ttcaacatct tcatgttctt ctttatgaat cccaacttct
    26761 tcacgaataa ctgtatgttt gcttacatct acttgctctt ctttgattgg gatagtaatc
    26821 gtgtcatctt ccaatttttc gtctgttccc tgtgaagatt gatctgttac tggtttgcgt
    26881 tcgatgacga tttcttcacg tttgaccggt acttctaccg tttcagtatc gttgaccaca
    26941 tgtttgtgga tgtcgacttc tccagttgtc acatcatgtg tatttacatc taatttttct
    27001 tcttttagtt gaattgtatc atcatctaga tttggtttgc gatcgatgtc ttcagtcttg
    27061 cttgtaccca ttgtatcagt ctcgccaggt acgtccatcg gatcagttcc tggtgtcata
    27121 ttcaatggat ccgttgtatt ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt
    27181 ccagtgccag tttctaatcc agtccccatt gtgcttgctg ggctaacagt atcttgtgta
    27241 tccattgtat cttctgttgt ttctggctga cgatagcctt ttactgtgat gacaaatttt
    27301 ccatcagaaa tatctcctct atattgatat aaaggatcat tttctggtgt agccgtctca
    27361 gaatcatagg tatcaaaaga aaaagtatct tttatttttt cccataaaga acggtcttct
    27421 tcaccatcca tccgattttc atctgtttca acatctatac cagacaatcc gtcaaatcct
    27481 agtctagatg ctgcttcatc agtagtataa ataacgatat catctcgttc atacccttcg
    27541 ttacgcaacc gttcaattac tgataatgct tcttccctcg tactatagct tccgatgacc
    27601 gttaaatttt tctcttccat gtaaattcct cctagcaaat agatttatga ttggtatatt
    27661 gttacttata aatttattct
//
LOCUS       DACDSR010000028        26907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDSR010000028 DACDSR010000000
VERSION     DACDSR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.028"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(60..1538)
                     /locus_tag="IWK15_001249"
     CDS             complement(60..1538)
                     /locus_tag="IWK15_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9546847.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1836..2771)
                     /gene="murB"
                     /locus_tag="IWK15_001250"
     CDS             complement(1836..2771)
                     /gene="murB"
                     /locus_tag="IWK15_001250"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9546848.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2844..3764)
                     /locus_tag="IWK15_001251"
     CDS             complement(2844..3764)
                     /locus_tag="IWK15_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9546849.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3877..4629
                     /gene="xth"
                     /locus_tag="IWK15_001252"
     CDS             3877..4629
                     /gene="xth"
                     /locus_tag="IWK15_001252"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9546850.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5493..6026
                     /locus_tag="IWK15_001253"
     CDS             5493..6026
                     /locus_tag="IWK15_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9546851.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6516..7040)
                     /locus_tag="IWK15_001254"
     CDS             complement(6516..7040)
                     /locus_tag="IWK15_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546852.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7033..7506)
                     /gene="tsaE"
                     /locus_tag="IWK15_001255"
     CDS             complement(7033..7506)
                     /gene="tsaE"
                     /locus_tag="IWK15_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9546853.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7629..8609)
                     /gene="pta"
                     /locus_tag="IWK15_001256"
     CDS             complement(7629..8609)
                     /gene="pta"
                     /locus_tag="IWK15_001256"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9546854.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8762..9442)
                     /locus_tag="IWK15_001257"
     CDS             complement(8762..9442)
                     /locus_tag="IWK15_001257"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9546855.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9568..10437
                     /locus_tag="IWK15_001258"
     CDS             9568..10437
                     /locus_tag="IWK15_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9546856.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10463..10978
                     /locus_tag="IWK15_001259"
     CDS             10463..10978
                     /locus_tag="IWK15_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546857.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11025..11588)
                     /locus_tag="IWK15_001260"
     CDS             complement(11025..11588)
                     /locus_tag="IWK15_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9546858.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11643..11822)
                     /locus_tag="IWK15_001261"
     CDS             complement(11643..11822)
                     /locus_tag="IWK15_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546859.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11899..12408)
                     /locus_tag="IWK15_001262"
     CDS             complement(11899..12408)
                     /locus_tag="IWK15_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546860.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12504..12884)
                     /locus_tag="IWK15_001263"
     CDS             complement(12504..12884)
                     /locus_tag="IWK15_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9546861.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13214..13963
                     /locus_tag="IWK15_001264"
     CDS             13214..13963
                     /locus_tag="IWK15_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9546862.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14147..15424)
                     /locus_tag="IWK15_001265"
     CDS             complement(14147..15424)
                     /locus_tag="IWK15_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546863.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15503..16147)
                     /gene="pcp"
                     /locus_tag="IWK15_001266"
     CDS             complement(15503..16147)
                     /gene="pcp"
                     /locus_tag="IWK15_001266"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9546864.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16346..17290)
                     /locus_tag="IWK15_001267"
     CDS             complement(16346..17290)
                     /locus_tag="IWK15_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9546865.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17292..17978)
                     /locus_tag="IWK15_001268"
     CDS             complement(17292..17978)
                     /locus_tag="IWK15_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9546866.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18452..18772
                     /locus_tag="IWK15_001269"
     CDS             18452..18772
                     /locus_tag="IWK15_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9546867.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18926..19831
                     /locus_tag="IWK15_001270"
     CDS             18926..19831
                     /locus_tag="IWK15_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9546868.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19828..20601
                     /locus_tag="IWK15_001271"
     CDS             19828..20601
                     /locus_tag="IWK15_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9546869.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20782..22206)
                     /locus_tag="IWK15_001272"
     CDS             complement(20782..22206)
                     /locus_tag="IWK15_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9546870.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22417..22863
                     /gene="nrdI"
                     /locus_tag="IWK15_001273"
     CDS             22417..22863
                     /gene="nrdI"
                     /locus_tag="IWK15_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9546871.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22928..24910)
                     /locus_tag="IWK15_001274"
     CDS             complement(22928..24910)
                     /locus_tag="IWK15_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9546872.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24924..25820)
                     /gene="murQ"
                     /locus_tag="IWK15_001275"
     CDS             complement(24924..25820)
                     /gene="murQ"
                     /locus_tag="IWK15_001275"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9546873.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25823..26884)
                     /locus_tag="IWK15_001276"
     CDS             complement(25823..26884)
                     /locus_tag="IWK15_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9546874.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         7857 a         5566 c         4706 g         8778 t
ORIGIN      
        1 tttgaaaaaa acataaatca gctaataaat atgtgagaca aagctgccat ccataaatac
       61 tagctgattt tttcagttaa atgtctattt ttttctctga tgatggtatt tccaaaatat
      121 tcccagtcat ctggagtcgg tggatttttc caaattatct gttctttatt aaatttttca
      181 agtaaacaat cagataaata aatattcgta cgagaagtga ttcgactctc aatcacaata
      241 ttgtaatttt taaatccttt cacttgagaa ataatgtaat cggtataagc agatccttgc
      301 gaaaaatcaa tacagacatg aatcggtgct tcaaaatatg ataccggaca aataccgacc
      361 aataaaaaga gataatcata aaataagcga atcatgaaac gttcatcttt tcccaagaaa
      421 tagcgtttat tctcttgtat gaccttttgg attgctgtac tgaatattgg atacgtctcg
      481 aagaaaaact gtagctgctt tttagacgta aagctagtca tctcaaagtc gaatatccaa
      541 ttttttcgat tgacatgcat catacccata cgaagttctt ttcgtatata tagtctttct
      601 tcttcagaga tttcaatcga aaatggtaat tgatcaatca attccactga cattttttga
      661 ctaatcatgt ggatatcatg aaaatgagct tccgcaagtc caaatgcgcc atctaattca
      721 aattcttctt ccgtgatcaa aaacaacatg atataagaaa tctcatcaat gatctgctct
      781 ttagggatat gaagaaaaga aatatgtgat aaatccttga tgatcttcat tccttctgag
      841 ctcttaaagt attcatctga aaagaaacgt tttccgattg gttgattccc ataggccctc
      901 aacaaactaa tagtggttag caaattgaat ttgattcgtt ttgttgcaga cagttttaaa
      961 ttgaacagat attcaaaata ttgcacgata ctctttgctt cattcatgac gctttctcta
     1021 aatggcatag aaaaaccatt aaagaccccg aaatataccc caaataatac gttccgtatg
     1081 tttatttctt ccccaactaa tatgtttttc tttaacttta tttgatgctg ttcaagaaaa
     1141 gagactaatt ctcttctttt tacataggct cttgatatgc ttaaaaaatg attctctgca
     1201 aatttttcga tcgttacttt tgaatccacg atctccgtta acagtcgagc aatttttgat
     1261 cgttgaaagt agattcttct taaatttttg atatccgtca aattcagacg ttcgacatgg
     1321 atctctgctt cttctgaaat ttggatctct ggctttgttt tgaattcagt aaattcttta
     1381 tttaattcta tgatataatt tcttgtttta aatttagaca aacctaatgt ttcatataac
     1441 ttaggcagca ctacataatt ctttttctca aattctaaat atctagctag cagcagtttt
     1501 tctctatctt cctcttctat aaggtaatcc aacttcatat cctacacctc aaaaaatgcc
     1561 tcataccaat tacttgatat gcagatgaaa gttcatcttt tcacctatac tgattttatg
     1621 atggattatg cttgtttttt catcagttat tctcattttt tatctttttt ttgagaatat
     1681 tgcaaaaaat ggaaaaattt atgaggaaat attctatatt ttttgtttta ttgtgttaaa
     1741 atgtacaaga tttgcgccat ttgcgtaaac aaccacaaaa aagagcagga cggccaccgt
     1801 cctgctcttt tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt
     1861 tctgatattg cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat
     1921 ttttctttga ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca
     1981 tgatcgatat tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc
     2041 aggccttgca atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta
     2101 aagacacttc cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac
     2161 tcatccattc ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc
     2221 aatacgatag cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct
     2281 tttgtcaaag ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct
     2341 ttgatctctc cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg
     2401 atgccgcaag caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca
     2461 atcagtgagg cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt
     2521 tcagtcagca tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca
     2581 tttcctaata ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact
     2641 tcttcttgtt ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta
     2701 tagttcatca atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct
     2761 tctttattca ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct
     2821 taatacaatc ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc
     2881 tcacataatt catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta
     2941 cctaaacgga tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc
     3001 gtgtttgtcc agtcgccaaa ttctttcact ttttttccat cagcctcttg aatgacaagc
     3061 tgggtcagat cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata
     3121 ttagcgccac ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt
     3181 aaaatggcat attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg
     3241 ggttcatcta ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg
     3301 aactctgtcg tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct
     3361 gggatacgtt tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa
     3421 attatttggt tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc
     3481 agtggtttgt caatataaac atggatacca ttttctagac attttttggc taattcataa
     3541 tgtacttttg ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg
     3601 atcgtttcat atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta
     3661 tttcttgttg caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat
     3721 gctttttctg cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga
     3781 tcatttagtt actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca
     3841 aaggtaagga aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta
     3901 aacgggcttc gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc
     3961 gattttttct gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca
     4021 ggttattatc aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt
     4081 gctaaaaaac cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa
     4141 ggccgtctga ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat
     4201 tctcaaagcg aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat
     4261 tatttggaaa atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct
     4321 catcaaaaga ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca
     4381 gaagaaagag cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat
     4441 ttttatccaa cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa
     4501 aacaatgctg gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta
     4561 gctgatgcaa agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat
     4621 ttgaaataaa gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga
     4681 aaatggcttt ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca
     4741 ctgtttatcg agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca
     4801 aaaatagttc ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga
     4861 acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa
     4921 acaaaaatag ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
     4981 tgaacaccgt ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc
     5041 caaacaaaaa tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc
     5101 ttttgaacac cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa
     5161 acaaatggca aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc
     5221 tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga
     5281 aatttttttg aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc
     5341 atgatcccca tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca
     5401 aatattacat aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg
     5461 ttacagtaat ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa
     5521 ctgccaatca tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc
     5581 agatcgttga tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg
     5641 tccaaattca cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct
     5701 ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt
     5761 ttgatacaaa ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc
     5821 tttctttatg tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat
     5881 tgaacactgt gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag
     5941 atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat
     6001 tgaaaaaact tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga
     6061 gcctgccgtt tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat
     6121 taaatattgt gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg
     6181 tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt
     6241 tttcatttgg ttggacttat taccgcagat gcaagctttt ggacaccgtt taccaagagg
     6301 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggcaaaat agttcctcat
     6361 attttgccgt tttttgagct aataccgaag gtcagctgat tgaacaccgt ttattcaaaa
     6421 aaaatggact gcgacacgac ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa
     6481 aaggatgaca gtttctcact cttttcctat ttcttctatt caatcaaata actcatttta
     6541 tatacatcta aaaattcacc tagatctgtt cttgcccctc taggcatgac cgcttcgatt
     6601 tggaagccca tttttcggta aaggtgtacg gctctttcat tacgaacttg aacgtcaagc
     6661 tccaatcgaa aaagtacacc catttccttc gcccaactga tgatttcttc tagcataaga
     6721 gtgcctagtc ccattcccca atattcctgc agaatactga taccgacttc tcccacgtga
     6781 gacaaacgaa attgactgtc tgccttgaca gatgctgttc caataatcgt cccttcatag
     6841 attgcaagca gcaataaatt attattgctt tcgtatatat aatccaactc ttcttctaag
     6901 gtttctggtg gtaacaggag ttctgcttca tccaaaacta aaaattctgt ctccttatta
     6961 acttgtccca tcatcaaaag aatgtctttt gcatctcctg gcactgcttc ccgcaagatg
     7021 atcccaactt cttcactcat gattttcagc ccatttttct agaattcgct gtttgaaggt
     7081 ttcactttgc tccccgtatg agattagttt cacataccgc ttttctaaat ctgtatcact
     7141 tttttctaaa atcagttcca aataatcctc cggcagtgct tcttctaata gatttcccca
     7201 ttcaacaacg gaaagcccat caccttcaaa atattcatct aaccctaaat ctgctccgct
     7261 agctgctacc cgataaatat ccatatgata caacggaatc cgaccttgac tatattctct
     7321 aataatcgtg tatgtcggac ttttgatcat ttgcgttatc cctaatcctt gggcaattcc
     7381 tttcgtcaac gttgttttac ccgcacctag atcaccagtc aatatcagat tgtcacctgg
     7441 cattgcggaa tgaccaattg cctctgcgaa ttgttctgtc attgttaatc ctgatagttc
     7501 gatcattttt catcttctcc cgctcttctt tctttttgcc agtatatcgc gtaatgaagt
     7561 ttttttaaag aaaaaatgaa ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg
     7621 tgtatttgtt aatcaatcaa tgtttgtgct gctgtgataa tcgataattt atatacatct
     7681 tcttcatttg ctccacgaga caaatcagaa accggtttgt tcaagccttg tagaattggt
     7741 ccgattgctt caaagttacc gaaacgctga gcaattttat agccgatatt tcctgactgc
     7801 aattctggaa atacaaagac tgttgcttga ccagctactt ctgaatctgg cgctttcagt
     7861 tgtgcaactg aaggaacata agaagcatca aattgcaatt cgccgtcgat catgtattgt
     7921 ggcgctagtt ttttagcgat tttagttgct tcaactactt tgtctacttc tggcgatttc
     7981 gctgagcctt ttgtcgagaa gctcatcatt gctacttttg gttcgatatc gaacaattct
     8041 gctgtttttg cagaatctac ggcgatttca gccaattctt gcgcagttgg attcacgttg
     8101 atcgcacaat ccgaaaagac atatttttct tgatctcttc cacgtaccat gatcatcgct
     8161 ccgcttgtcc gactgatgcc aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg
     8221 tcgccagtcg aatggatcgc tccagagacc attccgtctg caatccccat atacgtaagc
     8281 atcgtaccaa agtaattcac atcttttaaa atcttttctg cttgttcttt ggatgccttt
     8341 ccatttctac gttcaacaaa agtatccacc atttcatccc acttatcgta attatcaggg
     8401 ctgatgatcg taaagctaga tgttttgatt cctctggcat gcgctgcatt tcttatttct
     8461 tcttcgttcc ctactaaaat gggttccaca agttcttctg ctttcaaccg ggcagctgcg
     8521 cctaaaatac gagcatcagt agcttccggg aaaacgattt tgatcccacg gcgaacaatt
     8581 ttaaatctta aactatcaaa caattccaca agatgacctc cagacgtttt tatatgtatt
     8641 atcatacact attccagaaa aaaagaacag tttattttat ctgtatcagt caaaaatgct
     8701 ggaaaaatta agtttttcac atacaaagcg aactgcacta taacaggaaa gcgctgtttt
     8761 attacactgt ttcaggcagt tgccaatcaa tcggttcttc tcctaacgca aggagcgcat
     8821 cattcgtttt cgagaagggt ttggaaccaa aaaagccacg acttgcagat aacggactgg
     8881 gatgtggaga tgttatgatg atatggcgac tcttatcgat cattttgatt ttttcttgtg
     8941 ccggttttcc ccacaagata aacacgattg gtttttcccg ttcatttaat ttttcaatga
     9001 tcgtgtctgt taatctttcc caacctttcc cgcgatgaga atacgcttgc ccttcccgaa
     9061 cggtaagaac ggtgttgagt aaaaggactc cctgtttagc ccatgacact agatttccat
     9121 gttgaacagg tgcgattcca agatcatcat aaagctcttt atagatattt cttaacgatg
     9181 gcgggatctt tacgccaggt tgcacagaaa aagataagcc atgagcttga ttttctccat
     9241 gataaggatc ttgtcctaaa atcaccactt taacttcttc ataaggagtc aattccaatg
     9301 cttcatagat atgatacata tctggatgga tcttttggga cgcatattct ttttttaaaa
     9361 attctcttag atgcagataa taatcttttg aaaattctga ggacaaaact tcttgccaac
     9421 tgttatgaat gatttctttc aaaaactcca cctcctaata aaaagaataa ctcacatttt
     9481 cttcaatcac aagggacaat cttcaccctc agcacttgat taatatggta aactaggaac
     9541 aagaagcaga aaggaacgat aatcattatg atcaaattaa ttgcatcaga tatggacggc
     9601 acattattag atgcccatat gagtgtttct cctgaaaatg ccgaggcgat acgttttgca
     9661 aatgatgccg gtgttgagtt catggtcgct actggtcgaa actatcaaga agctcgcgca
     9721 gctttagatg aggtaggaat cgattgtgcg atgatcactt tgaacggggc acaggtattt
     9781 gataaagaag gaaattcatt gtttaccgtg tcgattccaa ataatcaagc aatctctgtt
     9841 ttagatattc tagatgctaa cggtatttac tatgaagtag caaccaacga tggtctttat
     9901 tctgaaaatc aagcgaaacg aattgaaagt tttgcttcca tggtggctac ccatttacct
     9961 caccttacgt ataaaatggc gattgccatg gcctcagcta agctggagtt attgcacatc
    10021 acatacgtgg acagtattcg tgctattttg gaagaaaaga atctggaagt tttaaaaatt
    10081 atttgttttc atacagaagg gccagctgta ttaggtccag ttggaaagca gatttctctt
    10141 ttaggcgatc tagccgttac ttcctctggt caaaacaata tcgaagtgaa ccataaaaac
    10201 gctcaaaaag gaattgctgt tgcccatgtc gctcacgagc gtgggatttc tttagaagaa
    10261 gtcatgacga ttggcgataa cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt
    10321 ttcgcaatgg gaaatgcaga aatcgaagta aaagattatg ccaaatatac aacggacaca
    10381 aaccttgaat ctggcgtagg aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt
    10441 tatctagaaa agagggatat agatgtcaga atttttcatt caggaaaagc agcttggacg
    10501 tgcaactcgg acaattgtca aaaacgaaaa aggacattcc ctgtttttga tggttggtcg
    10561 ctggggaaca aagggcgatg cattgtctct ttatgccatg aatggcgatc tcgttgctca
    10621 catcaagcag ataagcctga cattcggtac gcgttttgag ctgtataaag agttcgaaaa
    10681 ggtgggaacc atgcagaaaa tattcaattg gcccggtgac ttttattata ttcgtagact
    10741 tcactggaca gtccaaggtg atatctataa tcatcaatat caaatccatc atttcaatca
    10801 gcaaatcatg aaaatggata aagcaacact tttttcagga gattactatg tattggatgt
    10861 ctcaaatgat gaagatgctc cagtttgtat ttgtattgct gcggttttag attactggct
    10921 ctaccgtaaa gacaaaagtc gcgataagcc tgtttcttac cgacttaact taagttaaaa
    10981 agatgtacct gaaatgcgga aatacgcatt tcaggtacat ctttttagat attcttttgc
    11041 atatgatcat aaagatgacc actgatcatc cgctgaccga caactttgaa gtcatggcgt
    11101 tcgtataatt ttttagcctt tggatttttt tcatccacac ttagaccgat gacggagcga
    11161 ttagctttct tcgccaattt aggtaatgct tctaataatc gcgaaccgat gccttgtcct
    11221 ctaaaatctt ctgatacact gatagagtcc aaataccatt cgttgggaaa cgtttcttta
    11281 tcaatgaaca aacgtgtaga cgcatctagc tgatatttct ctaagatagg taccagtggc
    11341 tgatcgatga ttgattcttc ttcatctgta taaccgtaag caactcctgc aactttaccg
    11401 tctatttctt ctacgatacc tcgtgaataa ctgtaacgat aatttggatc agtgattgct
    11461 tcttctaaaa tctctaaagt cttttgttcc cctgtctgct tcaaaaatgg cagttccata
    11521 tcttttagaa tcactaagat caatggggcg atttctggag catcttcttt tctcgcaaaa
    11581 cgaatcatag tttcctctcc ttttctccat tctaacatgc agaaaaagaa atcgcaaaat
    11641 cgttatttta aaaaatcttc aatcttttca gtaatgaaat aaccatgagt aacgattctt
    11701 gccgcttcag ttcggcaata agggcagacg actggcattt ctttatcatt atgttcgact
    11761 acattattga cttcttcgta tgcaaatttt tttccgcaat tagggcattt cattttttcc
    11821 atgttatctc aacttccttt catctttact ctcttagctt actcttatat tgtaaaaaag
    11881 agaagcaata tccttcttct atgcagatat ccatacctga cggtcctgtt ctccatctat
    11941 taatcggact ttataataag gacgttcatc tttgactaga agtgtccact ctttcacttt
    12001 tgcttttttc ccgtagtagc tgactgcttc cttcatcgct gcttgcggtt gtttgaactc
    12061 ttccgttttt tctatatcaa aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt
    12121 tgttttccca gtggatggat tgactgctat gacttgtcct tcacaaaaaa actggtattc
    12181 atatgttgca ttcgtaggat tctccaaatc tttttcaact gcaaaatgaa tagcagatat
    12241 gttatgttct tttgtttcgt tttttacgat tgctgctgct tttttcaatg agatccgata
    12301 ttcctggtca ctaatatcat ccatgtgaaa agcggcattg agcagtcccg tcataccggt
    12361 aatccctatt acgatgaggg cagcaacacc aaacatcatt tttttcattc ttcattctcc
    12421 tgtttctttt ttttaaagaa tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa
    12481 aaaaagtgac aattttgtta tctttattcg tgcagttcta atggcaatcc gtctggatca
    12541 aagaaaaacg tcattttttt tcctgtaaag gtatcttctc taatcggttc tgattcgatc
    12601 cctaaggact gcaattctgc aattgtttct tcaatatcgt ctactttgaa agcaagatga
    12661 cgcaagccac aagcttctgg atagtttggt cgttgtggcg cattagcaat tccaaaaatc
    12721 tccaattcag cacttcctag tttcagatct aatttatagt catctcgttc tttgcggtaa
    12781 ttttctctga ttacttcaaa tcctaattta ttaacataaa aatcacgtga tttctgataa
    12841 tctgaaacaa tgatggctat atgatggatc gttgtaaggt tcatatgagc actcctattc
    12901 ttcgtttaag ctcggatcta tattaatctc cagttccatt gtaaatgtgt cagattgaaa
    12961 aaaatagctg tttttccttt ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc
    13021 caagacactc ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa
    13081 aacaaacaga aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca
    13141 agaaaagcat acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa
    13201 ggagtcctca tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc
    13261 gcaacaaaat tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa
    13321 tagtcccatc acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg
    13381 tattttatcc atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat
    13441 ttgttttatt atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc
    13501 ttatatgatc acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc
    13561 ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct
    13621 tgtctatctt gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt
    13681 agatgccatt gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa
    13741 caaccaatat tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc
    13801 atttacgcat ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa
    13861 tgatgtcctt gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca
    13921 aaaaatggaa gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga
    13981 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag
    14041 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    14101 cttcttattt cttggagtta aacgcttctg tcccagcctc tattttttat agtaattcct
    14161 tcagatactt tcttgcctca tcattgaaac gcagtgattc ttctaacttc tgataatatt
    14221 ccaacgcttg tttctgagct tcagtaaaac gataatgttt tcctttccat ttgtcgattg
    14281 tctcttgcac atttccagat tggatatctt tcactactag ttgccatttt tcttttgaga
    14341 ctgtacggtc tttcttatca gcagattgta tagcttctcg ttctttacgc aatactttta
    14401 aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc
    14461 gacaatattg cttccaaata aatagataga tttttggttg gatgaatcgt tggtttttcg
    14521 caaaatattc catctgagca gtgacttctg gtgttcgttg acgttttgtc tttctcaaca
    14581 cggaaataaa tgtttcaaaa taagcattag gatgtactgg cacttcttca tatacgatgg
    14641 attgccaatc tttacgtaaa aatgcgcgat agttgatcaa gaattcttct acatccttta
    14701 ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc
    14761 cattgatgtg ttcacttatt ttcttgattt cattttccaa tgcttgataa tccgctatgt
    14821 aaatcggcat ttctacttgg cgaaattctg cgagccgctg agctaatttt atatccggta
    14881 aatatggctg tttcatccga cgattttggt acaagacaaa acagtatttt tgccatagtc
    14941 cttcaggaaa atcgatattc ttctgcttaa gccataataa ttcgtctaag gccagatcaa
    15001 cttttgtcat tccctgatgg atcgaagctg ccactgtcgg agagacatgc aggtgatcgg
    15061 caaagtggtt gatgcctagt tttaggatct ttccggtttt tggcgattta acgatgtatt
    15121 gcacttttaa ttctctacca cactcacaat acaatttttg ctggcttcgt tttgttcgaa
    15181 aaaacggatt gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa taatgttctt
    15241 ccataaaacg agagctgatt agatatcggc ggtgcttatc gactagttcc cgctgttttt
    15301 tcgttaattt tgcatagatc tcccaacgtt gactgcttgt gtattgttta gaaattttga
    15361 tgggatagct tttgctgatt tcttttggaa accattcttc aaagacggat tgccattctt
    15421 ccacggctgt ttctttcctt tctaattaca aaaagctggc tactaacata acgttagcaa
    15481 ccagccagtg tttcttgcta aattagtgag ttgttccggc atttccggaa atatcttctt
    15541 tattttctac tgctgcttca actgcataag tcaaagaaga agcaatgtct tctaaagaca
    15601 tagaaggcgt acccactgga cgatcgatca cttgatcggg tgagaatggc acgtggatga
    15661 acccacctcg aattgccggg aattgtgttt cgatcaaatg aagcaaacga tacatgatat
    15721 cattgcagac aaatgttcct gctgtataag agatgtacgc tggtagacca tgatcttgta
    15781 cattttttgt cattgctttg attggcaatg aggtaaagta agcggctgga ccgttttctt
    15841 ctaaagccgt atcgaatggc tgatttcctt cattatcagg gattcttgct tctgctagat
    15901 tgattgcgac acgttcgaac gagactgctg accgcccacc tgcttgtccg acacagatca
    15961 cgacatctgg ttggtgctgt tggatggctt ctttgactac ttgactgctt ttttcgaata
    16021 cggtcggtac ttccactttg atgatttcag caccgctgat ttcatctggc atttttttta
    16081 ctgcttcata ggctggattc actttgtccc caccaaatgg atcaaaacct gttactagta
    16141 ctttcatctg ttgttcctcc gtccgattga tttaacatat ggttatcttt tatcatacca
    16201 ttttccttga acagcgtaaa taaaggaaac aatagtttaa ataacataac agatcaaact
    16261 taatatattc ttataaaaaa atagtgttcc actacaatgt tagaagaggt tgcgacaatt
    16321 attctgtcac aacccctctc ttctattatt taaatagaat catataagca atttggaaaa
    16381 ctagcataaa taacgcaata aacaactggt ttttgatcac tccgtatttg tctttcatct
    16441 ccagcatcgc tacaggcacg atattgaagt tagcagccat cggtgtcagc agggtcccac
    16501 aaaaaccgca agtcaatgca accattccta ttaacgctgg attcgctcca tgactaaata
    16561 cgaatggtgc accgatccca accgtcatca cggtgatggc cgcaaatgca ttccccatga
    16621 ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat gccgatgttc acatttcctt
    16681 ctggaacgat cgtacctact gcattggaaa tcactgtccc aactccagcg cttgtaaata
    16741 ccgcacctag agaagctaaa agcattggca acatgcttaa cggccctact gtgcccagca
    16801 tatctgctgc atcatctaaa aatgtacttg gtcgattatc tcgagaaaag aacatcaaga
    16861 ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa ttggtaaata
    16921 gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg aataaaaatt ttcattccta
    16981 gtttgtctga cattttttcc atatattctt ttgatggaag ccggctttct cctttgctga
    17041 ctttcttagc aattgctggt atggtcatcg caaagatcaa gccaccgttc acgattgccg
    17101 gaagccatct tcctcctgca ataactaccc ctaagaccgt ccagaaaaag gctgacccat
    17161 aacgatgagg attggtttta tctgttaaat ttcttactcc agtgtaaatc aatacgagtc
    17221 ccatcacgat ataaaggatc tccagtaatt tatcgcctaa taaaatatca ggatttgtaa
    17281 aaaagtccat cttattgccc ctttcctgga taatatttct tcgctaattt cttttcttta
    17341 agatagaagt agacacttgc cacaactaaa gccgtgatag ccactggaac ttccactaaa
    17401 gcaagctgtc ccaatgttac gtcatatcct agatctttca gtgttgattg gactagcaac
    17461 gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc agcagacatt ttccatagct
    17521 gaagccatac cttttagttc ttcttcatgc ttaggatgga cttctaaccc ttttgattcg
    17581 atggtcccca atgccatcgg taaaatgatt ggacgaacaa atccagcaac tccaccaaaa
    17641 ctcacgttaa aagccgcaaa gatcattcga aagatcccat aaatatctac aatcatacca
    17701 gcagatactt tcttgaatcg cttgatcaag gaagccgctg attctttcag accatttctt
    17761 tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg attgtttaca
    17821 aaacttgtac ctaaaagttc taacattcct tgaatgccaa gaccgccagt caaagctgta
    17881 acaactaaag caaccattac gatcaatatc gagtctaatt taaatgcaaa cccgagaata
    17941 acaatcaaga cgcctaaaag gctaagataa ttttccatat attttcctcc ataaaatgac
    18001 aatatttttt taattataaa gcaaaacaca taaaaaatat agttgttttc tcacgaaata
    18061 gacactattt ttaaatattt ttcgttattt ttttatcaca aaaattatct tacttattgt
    18121 aaggtaaagc tttttttaat ttattgcccg ataatatata gaaataaggt ctagttactg
    18181 ttttttttaa atgattaata aataacaact tctttctatg aaattgcaaa gcattcatct
    18241 tcttttccgc taacgatata tccgtcagtg cttggtacac tttactaaaa agtatgagat
    18301 tgccgatatc tggataggtg tgctatacta cagaaaaaga tatacaatct gccaccgggt
    18361 aggataaaga ggatgatcat tccattaggt ctttgaaggg atgattttcc tctttttgtg
    18421 tattttttta tagttaagga ggaatgtaag tatggcatgg atggaattaa tcatagcagg
    18481 aattttggaa gtattctggt caacgatgat gaaatggagt gatggttttt ctaaaatcaa
    18541 ttattctttt tacacagtga ttggcatgat tgctagtttt tactttttgt caaaggcaat
    18601 caaaagtttg ccgatgagtc tctcttatcc tatctggaca gggattggcg cagttggttc
    18661 ggtcataatt ggtgtggtct tgtttcatga taagctgaat ctttccactt ggttctttgt
    18721 ggggatgcta ctcatcagta tcattggcat caagattact agcggccact agacacttgg
    18781 aataagtact gatcagaaaa aataagcctt tttatttaat attcaatttt tttatgaaaa
    18841 aataagaaat aagtccaatg acctagcgac ttctccactc atcaggtgta tgcttgtact
    18901 atctatagaa aaggagtgaa tatttttgaa aagtctttcg gtccaagaat tatccaagaa
    18961 ctttggtcac aaacaagccc tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt
    19021 tggattagta ggtcctaatg gcgctggaaa aacaacgatc atgaaagcta ttttaggact
    19081 gatccattac tcagatggaa caatctgtat tggtgaaaat gagatttctc cctctagtca
    19141 taaagggttg gaacaagtag gagcattgat cgaatacccg ggtatctatc cattcttaac
    19201 aggctatgat catctgaagt tattctctga aaccaacgat gtatctgcta tcgacactat
    19261 cgtgaatcag ctcaagatgg aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg
    19321 aatgaaacaa aaattgggaa ttgctcttgc cttactgaac catccgtcta tcgttatttt
    19381 agatgagccg atgaacggtt tggatccaca agcaacaaga gatgtgcgaa aaatcattac
    19441 cgacttggct actcagggca cctctttctt gatctccagt cacttactca gcgagcttga
    19501 aaaaatggtc gatgagctga tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat
    19561 ggctgaattg aaccaaacag cgcaagattt catcgttatc tctacaacaa atgatctagc
    19621 tgctcgaacg atcttgacag aagctggata tactttggaa gatcctgata aaatcaagct
    19681 tttaaaagaa aacgaagaat tattaggaac cgtgatccag ctattgtctg cacaaaatat
    19741 ccatgtgaca gacgtccagc atctgacgaa tgatttggaa tcttctctat tggaaattct
    19801 ggattcagta aagggggaag aagaaaaatg acattgcttg taaaacaaga attatttaaa
    19861 ttgatcaaga aaaagtcaac tgctgtttta tctgttctat tagttgtttt gctcatagga
    19921 acagcacttc tagccaaaaa atacacgacg atcattgatc cagttgaaat gactgctcag
    19981 ctttttagtg ccacatcttg gattgtcttt atcatgattg cagctgcaag cacgatcatt
    20041 tcgatggaag cacaatatgg tacattgaaa aatctccttt atcgaaagta ttctcgagga
    20101 gaaatattag taagcaaatg gatcacatta gttatttatt ctgtttattt atacctttta
    20161 gctattattg tcactgtgtt gatgaaactg attcttttcc cttcgatttc cttcacggag
    20221 aaggtttcta ctggtcaaac actgatccaa agcttgttca cttatacttt gggtagttat
    20281 atcggtttat ggctgatttt aagtttagtg ttgatgattg cttgctttat caatagttcc
    20341 ggcgcatcca tctcagcagg aatcgtcttt tattttgctt cgtcaatcat atctggtatc
    20401 cttattgcac tcatccaaaa atgggagtgg atcaaatgga atccgatcag tatgttgaat
    20461 ctgcaaaacc aaattggaaa cgaagagatc atgaagcaat tgactcatct ttctactaat
    20521 caaatgctat tcggtaattt agcttacatc gttttgttct tagcattagg atatctcgta
    20581 tttaagcgaa aaaatgtatg atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc
    20641 cttattgccg attggctgtc tcttaaacac cgtttattct gaaaaaggag ctgtgacaac
    20701 atttgtgtca cagctccttt cctagtatgt ttttgaataa acgacaggct cacaacctct
    20761 ctttatactt cttttatact tctatacgtg aagcatccca gtggcaaata aaaccattgc
    20821 aatcactccg accatcgtca cacctataag taaaatctta tctaagtgga tatgttgtac
    20881 aggctcgttt tgctccttac gtgctttgag gtaaaaataa atgcctggca agaaaagaac
    20941 aagaacaagc attacttcat taaaggctac gactagcata gcccatactt gaaaggcact
    21001 tgctaaaagc cctactaaaa attggaccca ttctttccgc tgataagaaa gtttcatttg
    21061 gtacaatcct aagaaaagcc acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa
    21121 gttataagca ctgtcagtga ataataatga aagtaaaaag acattcgtac ataacgtcgt
    21181 caatacgata gcataagttg gtgccccatt tttgttgagt tttccccatg ctttaggcaa
    21241 aagtccgtct tcagccatgt ttcccattgt ttcaatcggt aagatcgtcc atgagagcca
    21301 aacaccaact aaagaaatga tcaaaccgcc gttgatcacg actgctcccc atttaccaac
    21361 catcatttca aacacatagc ccatagcagg ctgcgccttg atcgtttgca gctgattcat
    21421 cgtcattgtc ccatatggaa gcatggaaac caagacgtaa ataataatca agccgaatat
    21481 ccctagaatc gttgctttcc caacatctga ttgttttttt gctcgtgaac ctacgacaga
    21541 ggctccttca ataccaacaa atacccaaag catcaccatg atgcaactca atacttgact
    21601 ggaaaacggg atcggctgtc cattcgcatt caatataatg tcgctagaaa agttatcaat
    21661 gaattgttgg atcgaaaacg tgctgatagc tactacaata aataaaaata atggcactaa
    21721 tttacagaat gtgacgatag cattgacgaa actagcattt tcgactcccc gattcactag
    21781 ccatgcatat ccccataaaa agactgtact catcacgatc gatgccagat tttgcccacc
    21841 ttcaaatact gggaagaaat atcctaatgc gctcatcaac aatgttgcaa aagctacgtt
    21901 gcctaaccag catgtgagcc agtaacccca accgctgata aaaccagcga aacggccaaa
    21961 accttcttct gcgtaactaa agatcccgcc ttttaaatct ggacgttttt tattgatatt
    22021 attgatcgac aaggagagca tcaacattgc tgttcctacg agtacccaac tgaaaatagc
    22081 tgggccgcca gccgatccgg agatgtccgt cataatacca aatacgccac ctccaatcgc
    22141 tgaaccaatt actaaggcag caagtggaat taaaccaatt ccttttttct gttgctctgt
    22201 atccatgtaa gcatcctcca aaataaaacc gcttttatca aaaagtataa caaacctttt
    22261 ttatttttac ttaattaata aaaactttaa tagaaatata attctaccgg cttttttctc
    22321 attatacaag caaagcgcct agtccctttc caaattacac caccaaaaaa cttaaaaaag
    22381 atgctatact aaaaggaaag aattggagga tgatccatga acattttata tatatcgatt
    22441 tcaggaaata cgcgtgcctt tgccaaacat ctagcagaat atgctgaaaa aatgcatgca
    22501 gaagatcctg tcaatcctga agttacttta aaagaaatcc atgaaaatag tgattttacg
    22561 aaagaaacag aaccattttt tactttcgtt cctacttatt tagacggcgg aaatggttta
    22621 gataacggtg atacagaaat cttgaccgaa acgatgcgtg aatatctgga acatgaagac
    22681 aatcataaac tttgtcttgg tgtagtcggt agcggaaata aaaacttcaa taatcagtat
    22741 tgtctaacag ccaaacaata tgcacaaaca tttggctttc ctttcttggc agattatgaa
    22801 ttacggggaa caccatctga tgtgacacga atttatcaga ttttagcaga aaacaataaa
    22861 taataaaaaa gaagaagctg aaaaatagat ttcagtttct tctttcgttt ctattgtttc
    22921 tatcatatta agctttgaca tccatcacag gtgcttttgc ttttgcttta cctgtttttt
    22981 ccaagacaaa gttttttaca cgatccatat tggttaccaa gacgatcatg gatgtttctt
    23041 tgcctgctgc tttgatcgct tctaagtcag cttctgctac aagcgtatca ggtgtcactt
    23101 gctgcccttc tgttactttc acatcaaatg gcgtaccgcc taagtcaact gtgttgattc
    23161 ccatatgaag caagatttct aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg
    23221 ggaagacaga gctgactacc ccagtaactg gagaatagat atgtccgttt tctggttcta
    23281 caaagtagcc ttcacccatc atcttttgtg caaaaactgg atcacttgct ttatctaaag
    23341 aaacaactgt tccgtcagca actgatgaga ggatagtcac actttcagct gaagtttgag
    23401 gtactacttt ttcaggcact tcatttactg tggctgtttc agtcgctaat tcttcttttt
    23461 ctaaacggtc tttaggaata ccgaagaaga atgtagcaac gaagccacca gcataagctg
    23521 ccaataatcc aaggatgtag cctaaccatt taccgtcaga gatcaatggg atcaaagcgg
    23581 ctcctgaagg tccaatagcg atagccccga catttccgat cattccgatc actgccccgc
    23641 cgatacctcc accgatacaa gcagtaataa atgggcgtcc tagaggtaat gttactccgt
    23701 aaatcaatgg ttcgccgatt cctaggatcc cgactggtaa agctcctttg atcatttctg
    23761 ttaattcttt atcttttttg cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg
    23821 caccagccat cgcaagaatt ggaagcaata gcgtacgacc agtttcatca atcatttgca
    23881 catggattgg agtcaaaatt tgatgcagtc cgaacataac catcggcaag aataatgtgc
    23941 ctagaacgaa gccggcaaat gctccaccaa cgccaagtac ccagttgatc ccaccgacca
    24001 tgccatcaga aatgaaacct gctaaaggca tgatgaagaa aatttcaatc agaccgatgg
    24061 caatcaatga aagagttggt gtaacgatga tatctaccgc atcaggtact actttatgca
    24121 gttttttctc aacgattgac aatagccaaa cagcgaagat gacacctagg atcccacctt
    24181 gtccagctgc aagagcttca ccagtaaata agtttttcag tggtgcttcc ggattcatcc
    24241 ctgtcagcat cacaacggcc ccgataactc caccaagagt tggtgttgca ccaaaaactt
    24301 gtgcagcatt gatacctgta tagataacta ggtagctaaa catcccgttt ttcaagatgt
    24361 tcatcacatc gatatattgt tgccatgttg ctgcatcaag tgttccggct gtgactaagt
    24421 ttgctaaaac tgcggcaatc cctgccacga tcccagtacc gacaaatgca ggaatcattg
    24481 gcacaaagat attagaaatg tcttttaata ccgatttgat tttagatggt ttttgtttcg
    24541 atttttgtgc cgctttcatt tcttgtgccc gttcgtttac acggtctttt ccagatgctg
    24601 atgaatttgc tgtagctgta cttacagaag aaagtttttc tcctaatttc acgcctgcca
    24661 aatcaaccat ttgctgtgct actttgttca ctttcccagg tccgatgatg acttgaagct
    24721 gttcatcttc tactacacca agcactcctg gaatcgcttt taattcagag acatttacaa
    24781 gatcatcatt gatgatcttc atacgcacac gagtcatgca gtggacaatg ctttctacat
    24841 tgcccatgcc gcctgcccct ttataaattt gttttgccag ttcttccaaa gtcagttcgt
    24901 tttttgccat ggtcttttcc ccctcataag gtccctttta caaatccgtt tgcttcagct
    24961 aattttgctt gagcttcctc tttatcacta tttgtcaaaa tcatgacgat agccagctta
    25021 acattttgat ctgcttcttc aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct
    25081 tgcatgatga ttcgttttga gcgttcgatt agtttttcat ttgttggttt gacatcgacc
    25141 atcaagttat tgaatacttt acctatacca atcatactga tagtagaaat catattcaag
    25201 ataagttttt gtgctgttcc tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct
    25261 gcatcgactt cgattgggaa atccgcgtgt ttactgattt cggcttgttt attgcaagaa
    25321 atcgttcctg tgacagcacc gatcgatttt gcgtaatcta atccaccgat cacatatggt
    25381 gtacgaccgc tagctgcgat accaataact atatcatttt ttgtcaaatt cagcttgatt
    25441 agatcttctt gccctagctc ataagaatct tcggcccctt cgacagctac agtcattgct
    25501 tcttctccac cagcaattag acctatgacc atttccggtt ctacaccgaa tgtcggtacg
    25561 cattcagcag catctaacac acctagtctt ccacttgttc cggctcccat ataaatcaga
    25621 cgtccacctt ttttaaagga ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt
    25681 tctttttcaa ccgcagcggc cactttctga tcttctttgt tcattagttg aacggcttga
    25741 gaaatactca tctcatctaa tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc
    25801 tctaaattga ttttgctcat ttttatcttc cttcttttct tataaattga tagttcatac
    25861 cagctttgat ttggttgatc aatggtagct cttctttgat aactttagct actacattca
    25921 ctttttcatc cgctggcaaa tcatatttgg ttaattggat ctcacccata tagcgcaaat
    25981 acttttcgtt atctaaagta atagaccctt tcatacgcgt agctgtattt cttgctggga
    26041 taggcggaat ctctcgaaaa cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt
    26101 gccgattcgt atgcttccct aatatatatt cgtaaaattc ttcatctaca ggttccgtat
    26161 ggagcaagac tgtctgttct ttttggaaag aagcaaattg ctcctgaacg ttttctgaca
    26221 gacctgcatc tccgatgtaa acaatatccg tgttacttgc taatagatct agagcgcctg
    26281 caagaggatg gatccctcga tgttcttcca aagttggcaa tccttgatat agtgggcctc
    26341 tcaactctgt atctcctggt acaaaacctt ggacaaggag accttgaatt ttgagccatt
    26401 gatttttttt ctgataccat tctttgtcca gccctgtttc gggacgtgga taataattat
    26461 gccaagcttc aatctgtgag aaatcagctt ctgcttcttt caattcatcg atatctttag
    26521 gcgtgatcgt gctagcattc aaactgattt tcaattgatg tgaccagtcg gcaatctgct
    26581 gattggaaat atgataatcc attcttaaac ctgttacacc aattgctttc aaaggcgtta
    26641 gatcttctaa tgaaaaaccc gctcgttcca atgcctctcc tgaaatatcg accatcaatt
    26701 ctagtttatt ctttttcgct tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa
    26761 catcatcttc tggtatatgc atggaagtga aaataccgac aaagccattg gatgccattt
    26821 ggcggatata ctgtttcttc tcttctgaca aagaatcgtt cataaatatt gaaaagccaa
    26881 acatattatc cctctttttc tgtatcc
//
LOCUS       DACDSR010000029        26819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDSR010000029 DACDSR010000000
VERSION     DACDSR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.029"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..495
                     /locus_tag="IWK15_001277"
     CDS             <1..495
                     /locus_tag="IWK15_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9546875.1"
                     /translation="SEVLPAIRKHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRL
                     IAEQKIAEYEPKISYLDSILSSTDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQ
                     WLLCKKHMNQGYTKSHTTEIPKADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDL
                     EEIG"
     gene            510..713
                     /locus_tag="IWK15_001278"
     CDS             510..713
                     /locus_tag="IWK15_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9546876.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            729..1049
                     /locus_tag="IWK15_001279"
     CDS             729..1049
                     /locus_tag="IWK15_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9546877.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(1231..2013)
                     /locus_tag="IWK15_001280"
     CDS             complement(1231..2013)
                     /locus_tag="IWK15_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546878.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            2064..2771
                     /locus_tag="IWK15_001281"
     CDS             2064..2771
                     /locus_tag="IWK15_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9546879.1"
                     /translation="MQELVILKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQK
                     YADLFWEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEK
                     AIRKNTAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVL
                     GGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRM
                     EIDQLNRQQRLFGDENE"
     gene            2949..3290
                     /locus_tag="IWK15_001282"
     CDS             2949..3290
                     /locus_tag="IWK15_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546880.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            3283..3954
                     /locus_tag="IWK15_001283"
     CDS             3283..3954
                     /locus_tag="IWK15_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9546881.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3960..4646
                     /locus_tag="IWK15_001284"
     CDS             3960..4646
                     /locus_tag="IWK15_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546882.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            4653..5504
                     /locus_tag="IWK15_001285"
     CDS             4653..5504
                     /locus_tag="IWK15_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9546883.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            5516..5785
                     /locus_tag="IWK15_001286"
     CDS             5516..5785
                     /locus_tag="IWK15_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546884.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            5947..6249
                     /locus_tag="IWK15_001287"
     CDS             5947..6249
                     /locus_tag="IWK15_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546885.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            6246..6407
                     /locus_tag="IWK15_001288"
     CDS             6246..6407
                     /locus_tag="IWK15_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9546886.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            6404..6709
                     /locus_tag="IWK15_001289"
     CDS             6404..6709
                     /locus_tag="IWK15_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546887.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            6709..7065
                     /locus_tag="IWK15_001290"
     CDS             6709..7065
                     /locus_tag="IWK15_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9546888.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            7025..7270
                     /locus_tag="IWK15_001291"
     CDS             7025..7270
                     /locus_tag="IWK15_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546889.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            7267..7686
                     /locus_tag="IWK15_001292"
     CDS             7267..7686
                     /locus_tag="IWK15_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9546890.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            7683..8240
                     /locus_tag="IWK15_001293"
     CDS             7683..8240
                     /locus_tag="IWK15_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9546891.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            8237..8533
                     /locus_tag="IWK15_001294"
     CDS             8237..8533
                     /locus_tag="IWK15_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546892.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            8610..9023
                     /locus_tag="IWK15_001295"
     CDS             8610..9023
                     /locus_tag="IWK15_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9546893.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            9369..9743
                     /locus_tag="IWK15_001296"
     CDS             9369..9743
                     /locus_tag="IWK15_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546894.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            9973..10317
                     /locus_tag="IWK15_001297"
     CDS             9973..10317
                     /locus_tag="IWK15_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9546895.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            10322..10603
                     /locus_tag="IWK15_001298"
     CDS             10322..10603
                     /locus_tag="IWK15_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546896.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10706..11020
                     /locus_tag="IWK15_001299"
     CDS             10706..11020
                     /locus_tag="IWK15_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9546897.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10998..12692
                     /locus_tag="IWK15_001300"
     CDS             10998..12692
                     /locus_tag="IWK15_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9546898.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12712..13890
                     /locus_tag="IWK15_001301"
     CDS             12712..13890
                     /locus_tag="IWK15_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9546899.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13853..14539
                     /locus_tag="IWK15_001302"
     CDS             13853..14539
                     /locus_tag="IWK15_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9546900.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14539..15699
                     /locus_tag="IWK15_001303"
     CDS             14539..15699
                     /locus_tag="IWK15_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9546901.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15709..16584
                     /locus_tag="IWK15_001304"
     CDS             15709..16584
                     /locus_tag="IWK15_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546902.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16581..16892
                     /locus_tag="IWK15_001305"
     CDS             16581..16892
                     /locus_tag="IWK15_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546903.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16882..17235
                     /locus_tag="IWK15_001306"
     CDS             16882..17235
                     /locus_tag="IWK15_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9546904.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            17225..17626
                     /locus_tag="IWK15_001307"
     CDS             17225..17626
                     /locus_tag="IWK15_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546905.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17619..18023
                     /locus_tag="IWK15_001308"
     CDS             17619..18023
                     /locus_tag="IWK15_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546906.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            18035..18643
                     /locus_tag="IWK15_001309"
     CDS             18035..18643
                     /locus_tag="IWK15_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9546907.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            18663..19025
                     /locus_tag="IWK15_001310"
     CDS             18663..19025
                     /locus_tag="IWK15_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546908.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            19028..19210
                     /locus_tag="IWK15_001311"
     CDS             19028..19210
                     /locus_tag="IWK15_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546909.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            19227..22646
                     /locus_tag="IWK15_001312"
     CDS             19227..22646
                     /locus_tag="IWK15_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9546910.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            22697..23434
                     /locus_tag="IWK15_001313"
     CDS             22697..23434
                     /locus_tag="IWK15_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9546911.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            23444..25735
                     /locus_tag="IWK15_001314"
     CDS             23444..25735
                     /locus_tag="IWK15_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546912.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            25759..>26819
                     /locus_tag="IWK15_001315"
     CDS             25759..>26819
                     /locus_tag="IWK15_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546913.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGST"
BASE COUNT         9711 a         3979 c         5665 g         7464 t
ORIGIN      
        1 agtgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
       61 gaagctttgc ttaatccaga tacaatcatt caattagcaa ctcaactaaa agaagaaaga
      121 actggaagat taatcgcaga acaaaagatt gcagagtacg aacctaaaat ctcctattta
      181 gatagcatat tatcttctac agattcagta acaattagtc agattgcagc agattatggg
      241 atgtctccac aacagatgaa taaattactt cataaactag gtattcagaa aaaagtcggt
      301 aaccaatggt tattgtgcaa aaaacacatg aaccaaggat acacaaaatc tcatacaact
      361 gagatcccga aagccgatgg tggcactaaa attgtaatga ataccaaatg gacacagaaa
      421 gggcgtctat ttatctacga attactaaaa aaagaaggat attaccctca aatggattta
      481 gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc actcaacgag
      541 atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac accatgctcg
      601 ctgaaagatt cggcattagc aaaagctact tatctttaat tttaaacggt aaaaagactt
      661 ctgcagaagc aaatagaatc atagattcga ttattactat gtacgaattg taaagggagg
      721 aacagctaat gcaatatcta gaagcgaaaa tcccagttcc agaaggctat gtaattatct
      781 cccaagtgga ttatgaggag ttaaaaaaag ctgatgatac tggtagatgg atgacgttgc
      841 cagaagtgct agaacggatt aacagaaaat atgattggtt tacttctaga gttttaaaga
      901 acccaagata tagaaatatt atcgatatcg aaaaaaacaa aaatggattt gtctattatc
      961 cagttgaagg aagagacaca tatctatttt taagaagtaa aacacttgaa tttttagaaa
     1021 caaatttttc ggaaatctta aggaggtaag cggatgggta aatttaatag agcactagta
     1081 ttcagcgcac cactaattat ctacgcttta ggtctttggg gaagcagaca agcattaatc
     1141 ggcacaattg tatatatggt ctggatcttt atggggcttg atgaagctga atatagagcg
     1201 aaaaagccag tcgggaggga ctgacttaac ctaattaatt aaaaattgta acaaataacg
     1261 caaaaataaa gctaatgaaa ttgtattgtt aggatctact ccaatagtta tatttacaat
     1321 ttcggttact actagtccta tcaaagctgc cccagtttgc tctaaatacc acattttatc
     1381 tttgtagaaa gcaggtgaag cttgttgttt ttcagatggt tcattagtta tatctttgtt
     1441 cttggctgta tttatttctt caaaaagctt tatagaatca tcaaggactt tcctttgctc
     1501 attaaggata tcaaaaaact tagcctcaaa ctcatcagat ggattagaag tgtctttaaa
     1561 aagtagattt tttgtactaa cttccggttc ggaagaaaat gctttttcta attcttgggt
     1621 agctacccct attctgaagt cgtacttaga catgtcaacg gcaaattttt ctgtgatttt
     1681 agagatatca gaaaaataag gttttggcat agtagctgcc atttttttag caatattaaa
     1741 acttggagat tctaattgtt tctgtattgc gaacaaagaa ggaggaatca tattagcaat
     1801 agatttgtta cgtttctcga caatttgaat gagagaatca ttgagtattg cttgcgaagc
     1861 tataaaagta tcttgaattg caattaattt tttagcttgt tctatcgcag gctgcatagc
     1921 gttaacgaca tctagagaag gagcaatggt ttcaccgatt aattttgaag aagctatcaa
     1981 tttttgataa taaggagtat ctgaaatatc caattatata caccaccttt ttacctaaat
     2041 tataccaaaa gggagagaaa aaaatgcaag aattagtaat tttgaaaaat aaagaagctg
     2101 tgactacgag cttacaagtc gcagaaacat tccgaaagga acatagagca gttttaaagg
     2161 cgattgacga tctaaaagat gggcttgcac aaaagtatgc agacctattt tgggaagatg
     2221 tgtacatcca tccgcaaaac aaacaggctt atcgaattgt ctatatgaat cgtgatggct
     2281 ttactcttgc tgtattagga ttcaacaata ctcaaaaagt tttagagttc aaattgaaat
     2341 acattgaagc gttcaacgaa atggaaaagg caattcgcaa aaatacagct tttgacacaa
     2401 ttgaagacat gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca
     2461 tgcttaaaaa tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa
     2521 atacgaaagt tatggaagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca
     2581 aaaaagtatt ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg
     2641 gcgagttacc tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttaccag
     2701 aaactgcgat tcgcatggaa atcgatcaac tgaacagaca acaaagactt tttggtgatg
     2761 aaaatgaata gagctgaagc gctaagaatc gggacggtaa ttgctaatcg ctggtggaga
     2821 cacaataaac caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa
     2881 ataaaaaagt gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat
     2941 tttagcatat ggaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg
     3001 gcaaatcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacggatg
     3061 aagaactagc atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct
     3121 atcttatcca tttatcgagg aagtccaaaa aacctgaata tatctatgtc atcgaagatg
     3181 gcttgattga tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg
     3241 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaacaaaggg
     3301 acaacgactt ttgaaaaact ttttagtcgc aagttaaata aaatactcaa gaaaaaagga
     3361 aattttgatt atttatcttg ggctcacgca tgggagatta tgaaaaagaa tgatccacag
     3421 gcaacggtaa ctattaatga gtataaacac tacagagttg tttctggaac tcatcaagac
     3481 tttcttgttg aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc
     3541 tcagtaacgg ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac
     3601 caaccagttg ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg
     3661 aaagcattgg ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca
     3721 ccacctagaa tcgatacgaa gaaattaaac atgctagaga cgattctaga agctttcaat
     3781 gagcagatgg gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat
     3841 aaattagggc tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt
     3901 gccttgatgg agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag
     3961 tgtttaaacc attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca
     4021 taggtgcaac catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct
     4081 acgatggtga tcgagttatt gaaattcgtt ttattgatcc tagacgtttc acagtacagc
     4141 aacgaaactt catctatgca ctcataggcg atattttcat cgatacaggc atgccaacgg
     4201 acttctggaa ggaattcttt tacttccgct ttgaaggtgt cacagggcgc aaaataagcc
     4261 tcaaagatga atcgaatacg actgtgagtg atgctaacgt cttagcaaat atcatcttag
     4321 atttcatctt tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc
     4381 aagaatatta cttctacaaa tgcatcacaa aaagagtctg ttgcatctgt ggcaaaacag
     4441 gagctgatat cgatcacttt gacaaagcgc taggaagacg aaagcgcaaa gaagttgatc
     4501 attcagagta cacatttgca gcactctgca gaatccatca cacggagaaa cacaagatag
     4561 gtgtgatcaa tttcaaaaat aaatatcaaa tcaaagggat caagttaaac caggaaacaa
     4621 ttaagaaact tagaatagga ggataaaaaa tattggctga caacaaacga tactactatt
     4681 taaaactaaa agagaacttt ttcgatagcg acgagatggt tcttttagaa agtatgccag
     4741 atggatacat ttattctaat attcttctca aactttattt aagaagtctg aaacatgaag
     4801 gcaaactaat gtttaatgac aggattccat tcaattctac aatgcttgcg actattacaa
     4861 gacactctgt gggagttgta gaaaaagcag tacaaatatt tcgtgattta caacttattg
     4921 aggtattaga taacggagca atttatatgt ctgatataca aagttttatt gggaaatctt
     4981 caactgaagc tgataggaag agaaaataca gaaaagaaat tgaggaagca aaacagaatt
     5041 taataacaag tggacaaatg tcggacaaat gtccggacaa aactacacca gagttagaga
     5101 tagagttaga gaaagatata gagttagaga aagatataga gaatgtaacg ccttcgaaaa
     5161 aatcgaaggc taagcccgtc cgtcataaat acggagagta taaaaatgtt cttttgtcag
     5221 atgagcagat ggagaaactc aaaatagaat tccctaatga ctaccaagag cgaatagaac
     5281 ggctatctga gtattgtgaa tcatctggta agacttataa aaactatttg gcaactattc
     5341 gaagctgggc aagaaaagaa aaaagtgaat ctaagaacgc aagcagtgga tacaagcgca
     5401 caggaagacg agagaagctt cctgagtggg caatcgacca agaagcctat ctcaagaaaa
     5461 aagcgctaga acgagctaat agacaatcaa aagcaccatt ctaagaggtg ggaaattgaa
     5521 aatcgattat ctagaactaa ttaatgaaat agcaaagtat aaaactggtg aggaaataga
     5581 aatcctgaga gacgtatatg atcaactcga agaagctgga atcgaaggaa ttaagaatga
     5641 tcgttcgagt tggagtaagc tcagatacta tttcgcactt tatatcgata caacacaatt
     5701 aagaaattta gcatatacca aattactatt tgttgattgt gttaaaggct tgcaaaaaca
     5761 tcttaatgaa cttgagcagg tgtaatcaga tggacctaaa gacatttaca gcacagattg
     5821 aactaatgca tcaagaagct ttaagacaaa gtgtatcgta cgaagacaag tggctcaaca
     5881 cgttccacgg cggacgtgag agcgcacttg atcaagtact caaattactg aaaggagaat
     5941 gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg aattgactta ttcagaagat
     6001 tggcaaaatg acaaagaagc tgcttcagaa gtgatgaggc ttggaagagc gatgtgggca
     6061 gacaagagca acaagccaag accacgaaaa atcgcaattt ggcacggtga caaacttcta
     6121 gtgataggga cagctgaaca gttagcaagt ctcacaggct tgcacgagaa aatcgtgagg
     6181 aaaagagcaa ggtgtggcta cacagacgct aagaagagaa cgtttagata cgtggaggaa
     6241 tcatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     6301 acgatctgga acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt
     6361 tgcacataga gaatttaaga agggaaatcc atcgattgga ggaatgagcg tggataagaa
     6421 agcagcaatg aaaagaattg ctgaattaac caagtcagaa tcttggcaag aagacaaaga
     6481 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc ccaaacggag
     6541 aacgccgaga aaaattgcaa tctggcatga tgaccgaatt ttagtaacag gtactgctga
     6601 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     6661 atggattgat tcaaaaggac gacagtttag gtatgtggag gagagataat ggatctcatt
     6721 acacaataca gtgacatcat cctcaagaaa atcatgatga agattcagaa agataaaaaa
     6781 tcaaaagaac gagctgaatt agttaagtta gaaatggctg aaacaggagc aggagtgcga
     6841 agtagcaggc attggaaagc agcagcaaac attgaatttt attacaacga aattcaaaaa
     6901 gggttcgatc agatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa
     6961 gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca
     7021 gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc
     7081 aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat
     7141 tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa
     7201 agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga
     7261 cagcgaatga taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta
     7321 gccgtcttgc attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct
     7381 accgaattga aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa
     7441 gataagaatg gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc
     7501 ttcgattact tagataataa agtctgtatt gtcaaaaatt caatagatta ttccggatta
     7561 gtttgtgcca ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa
     7621 gaatacacgt acgaagtcac cggaaatata tacgagaata gcgagttatt ggaggaacag
     7681 cgatgaataa acaggaagca atcaaaaagt tagaaagtat taaagcgata ggaaatgatg
     7741 caatagctgc ttgctataac gagagtataa attcaggtat tacgttaatg aaaaaaatag
     7801 acgaaccgca gaaaccagtt attccacaac tcgtggccgg ttggcttgag aaatctacgg
     7861 acccttttac aaaagctgaa aaaatagcgt atttaatcaa atctaaagat ggtgattcat
     7921 attatttctg tgattggttt gtacgagatg gcatagtgac gcaagagcaa ggagaagaat
     7981 tactcgcttg ggcaacaaga caatcatatg aaacactatt gagcctatac aacggctacg
     8041 aggttgagaa agagccgtta tgggcaataa agaatgccga tggaaactat cttactaaat
     8101 gtgctttatg gggaaaagat ggagtaaatt atagttttga atgcaatcca tctcatcgat
     8161 tgcttttcac tgataaagca acagcggatg ctgcagcatt gttggtgaat ggaacagtgg
     8221 aagaggtggt agaaagatga aactaaaaga cggattttac gctagcagtc acggtatcgg
     8281 cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgcaagaaat caaaattcaa
     8341 agtcggtgac atggtccgct gtgaagcaga agagttcgtt tatccgttca gaggatacgt
     8401 taaaaagata ctgtcaaact cagcaatcat tcgtattgaa aatacaatga agtgtgatcg
     8461 ttcgactgca aaacacaagc attatttagc tgtagcgaga ttagttgaca tggaagtaat
     8521 caagagcaaa taaaaaaagc cggatcgctc cgactgattc aataaatcca acacatttat
     8581 tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac
     8641 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag cggatggcag
     8701 gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag gcaccaaagc
     8761 acggcaataa ggcagaagac gcgatcattc agatgatgga tatagaagcg gagagagatg
     8821 cgattctagc ggctttgatg gctcttagtc taattagtcg ccagatactc tactacagct
     8881 tctgtgtgcc agatagcttc tcaaactaca ggattagccg tgaagtgggt tattcagaaa
     8941 gaagtataca acggatgaag tcggaagctc taatagagtt tgcagaagca tataaacacg
     9001 gaagaataat tgcttataaa taatttggcg gttttttggc ggaatgatgg cggtttttag
     9061 ctatttacca gtgatattat ggtagtgtcg aaagattagt gataggtcta agacaaaata
     9121 aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc ttcgatagac agcagcaaat
     9181 aaactaaagg atgtgggggt cagctcctac agttagttca tgtgttgctg tctattaatt
     9241 gcaactcttt cggttttatt gagtatttac tcaacttaaa aaaactgttg tttgtagatg
     9301 taagctgttc ctatttgtac atagtaaaaa atgatataat aaaaatatta cgaaaggggt
     9361 gagagtgcat ggaagacaaa atgtatttaa gacaaaaagt tttcacaaaa aaagatattg
     9421 aaaatcaaat taatggattt atacagaaat tagaaaatgc aaattcttat ttgataaata
     9481 aggaactaaa taaagcttat gaccagtgga aagaagtata tgatgagttg aaattgattc
     9541 aaaatgaaac aaaattagta cgaacggaga aaaagaatga aaatagtttc ttttttgatg
     9601 gatatgctat attgatgttg gaaactgtag caaaacaaaa tataaaagct cccaaaaaag
     9661 aactatctga caatattgat aacgcattag ccgagttaag gtattacgtt atgcaaataa
     9721 aagatgttag gatcactcgt tgagtggtct ttttattttg cacaaaggag gtaacaacaa
     9781 tgtatagacc acaatactta gaacagaagt atgaagtaat cactgtgcaa aatggtaacg
     9841 gagagatagt acgaaagtat agaagaccaa taaagagcga tacatataaa cgaaaggaaa
     9901 gcaatgaagt tattccattg tatggcaaaa gaatagctaa gcattaaata agattgtgaa
     9961 aggagacggg acatgaacga ggaattctat agatggctat tacagttgat aagagaagat
    10021 cgtttggtta agttctatca gtctcctaaa tggcgcaggc ttagagagaa agcgatgaaa
    10081 cgagatcact atgaatgcca agagtgtaga agactaggta agtatcatag agtagagaac
    10141 gttcatcata taaaggaagt caaggataga cctgacttag ctttagattt agataatctt
    10201 atttgtttat gtgttgaaca tcataatgaa gttcatggca gatatcttac agcattggat
    10261 aaacaagaga agaagataga aagctttgct aacttcgatg caagtgaaag gtggtaagtg
    10321 catgatcatc aatgataatg gcagagagta tgatacagaa aagattgaag agtattcatc
    10381 ttatactcag ggattaatta aacgtttgat atacgttcgc tatgtaggta ttagggatct
    10441 gttatcagat aactgttgta gtaaatacaa agtgaatcaa gtaagagaag cgttgaataa
    10501 agataataac gtcgaaagaa taaaaaatgt ttttggatat agtattgaag agattaatta
    10561 ttacattgac ttcgctgaag ctttcattcc gatggtgaga taaccccccc ttaaaataaa
    10621 tcgcaaattt tttgggggtg atgaaacgga gggggctgtc aggaaaagag attttttcga
    10681 actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa ttgtctcaaa atcgctacag
    10741 agaaaaatta cgccgctctt taataagcca attggaaagt cagaaaacaa atattgagcc
    10801 attcttagat aatgttgatc gttatatcag tttatgggaa acggcgatat cactggaaga
    10861 agatatatcc gagaacggca ttagactgga gaatggtaaa aagaatgaat cagtagcgtt
    10921 gcttgtttct gtcaacaaac aaatgggatt gatgttggat aaacttgcca ttactcctga
    10981 attggtaggt gaagcaaatg aatcaattcc tgagttataa gcatattgaa aattggttca
    11041 aagctataga agaaggcact atcaaggtat gcaaagagca attattgcta aaaaattatc
    11101 tagaagaaag agtctttact agagaagata tttacttcga taagcagatg gtagaggatt
    11161 caatcaatat accagcacaa tactttccat tcgaattaat tccgtgggaa aaatttctac
    11221 aatgttttat ttatggtgtt cgatggaaaa aagataaaac actagtgttc aatagatatc
    11281 tttcattaat gggacgtggt aatggtaaaa ctggttttgc ttcttggaac aacttctttc
    11341 tactaaccgc taaacacggt attaaaaatt atgatattga tatctatgcc aataatgaaa
    11401 gccaagcaaa gactagtttt gatgatgtat ttaaagtaat taaagatcat cctgatttag
    11461 ataaaaaagt atttaaagct acgaaggaag ttattcaaaa tatcgctaca aacagcaaac
    11521 ttcgttataa cacggcaaat gctagaacaa aagatgggaa gcgaccaggt gcaaaccgct
    11581 ttgatgaaat tcacgaaaat gaagattatt caatgataaa tgtggctact tctggtggtg
    11641 gtaaaattcg agattataga gaattttatg atacaactaa tggtcatgtt cgtggtggtc
    11701 cgcttgatga cattatagaa gaatcaaaaa tgattctttc tggagaactt ggaattgaca
    11761 aggatggagc agaattttct agtttgtttc catttatttg tcgcttggat aacgataatg
    11821 aagttgatga tcccgacatg tgggaaaaag cttgtccaac tattaattac aatgcaaatt
    11881 taaaacggaa aatgtttcaa gaatactctc aaatgcaacg taatgctggt ttaagactta
    11941 cgttcatgac caaacgaatg aacagaccta tggaagatac acgatttgct gttgcttcat
    12001 atgatgatgt tctgcatacg aaagaaaaag aatttcctga aaaaatggat gaagtgatag
    12061 gaacagtcga ttttgctgat agacgagatt ttgccagcgt tgggttgcta ggaaaatacg
    12121 ataaagatgt ttattttaca caacatactt ttatccacga atcagccctt cgattacaaa
    12181 acatcaaacg agaggttata gatatttcta tagatcaagg aaaatcacag atcgttcatg
    12241 gaaaaaatat agaagctgat tatattgtag gttggtttct tgaaatgagt aataaatatt
    12301 atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat attgaagccc gctttagaag
    12361 aagcaggttt tactgtggaa attgttcgaa gcggatctgt tacacatggt atgttaaaag
    12421 atctggttga tgaccttttt attaatcaac gtttattttt tggtgacgat gcgattatgc
    12481 gttggtattg catgaatgta tatgaagagc atatttctaa tggaaatata cgctatgaaa
    12541 aaatagaacc tgaaactaga aaaacggatg gctttttttc attccttcat ggtttgaatt
    12601 ttttagatga tatttatgat tctgctcctg taacagtcac aaatagctca gtagaaaata
    12661 caggaactgg atttactcct ctagtattct aacttgaaag gaggtgagaa agtggggatt
    12721 tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg ttcctttaga agaatacttt
    12781 tgtaaattgc aagttgattt tgtgtatcga aaatttgcga ttgaaacttg tattgatttg
    12841 attgcaaatg cgatgagtaa agcggaattc aagtcatatg aagatggaaa aaataaaaag
    12901 aatgatcttt actataggct gaatgtagct cctaataaga aaaataatgc aacagaattt
    12961 agaaaaaaac tgatcaggag attaatattc tacaatgaag tattgatcgt ttctccgtct
    13021 aataattcta gcgaaatatt tattgcggat agttgggatg tcacagaata tgcattgaaa
    13081 gatgatgtgt tttctcaagt gcaaattaac aacatagtcc ttgatagaga atttctagaa
    13141 agtgatgtta tctatataaa atacgcagat caacaaatta ggcaactagt cgatgcgtat
    13201 tatcaagcgt atgggaaact catttctagt gccatgaatg tttacaagcg ctctaacgct
    13261 cgtagatacg tactgaaagg gaatttattc cgaccgcaag acaatacaac acaagatcaa
    13321 atcaataaaa tgatgacatc acaatttaag gcttttatgg aagctgataa tgcaggtgcg
    13381 gtatttcaat tacaaaatga gtacacatta gaagatttca gcggaaactt ccaaagcaat
    13441 tcaagagata taaaaaactt aatagacgac atctttgaga tgacagcagc agcgtttcac
    13501 gttccgaaaa acctactaaa gggagacatg agtgggttat cggatcaagt ggacgctttt
    13561 ttaatgttcg aaatcatacc gattgctgaa cttattcagg atgcgtttaa cgctagtctc
    13621 tatgaagtag aagaatactt gtcagggaat tttgtacgtg tggatacaac tatgatcaag
    13681 attactagct tcaaagattt ggttgacgct attgatgtag gcattagaaa tggagtattt
    13741 acaatcaacg aaggaagaga acgcgttgga aatgatcgct ctgataaggc gatggcagat
    13801 gaaatattta taactaaaaa caatcaacaa gtatcgaaag gaggtgaggc gaatgacgac
    13861 aatgaaaaca tttctagcag taaagaatga aggcgcagta ccgcaaattt ttattcaggg
    13921 atttattggt tctagttggt tctttgaagg gaatactgac aagggaatca aaaatatttt
    13981 ggatagtcta ggtgatcaag aagaaattga agtagtaatt aattcaaacg gtggagacgt
    14041 atttcaaggg attgctattg ggaacttact taagtcaaat aaagcaaaag ttaacgttgt
    14101 gattaacggc ttagccgcta gtgctgcttc aattatcgca atggctggcg atactataaa
    14161 aatctacaac aatgcacaat tgatgattca ccgcgcttcc acatacggag aaggaaatgt
    14221 cgatgacttc cgtacgattg ctgaccaact ggaatcaatt gataaatcgg taaaggcttc
    14281 atataaaaca cgattcaatg gcacagatga agcattgcaa gaacttcttg aaaaagaatc
    14341 gtttatggat gcagaaacag ctttgagtta tggattggtc gatgaaatta tcgatgcaga
    14401 aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt gaagaaattt tgaatgacgt
    14461 taaagaaaaa agagcagaaa aaattgctgc atttacagca gcattaaata aaacatttgg
    14521 acaaggagat gtaaaataat gacagttaaa aatttaaaag gtgtaacagc tgcaagcgac
    14581 caattgatga aagcttttaa agatggtaac gaagaatctt ttagcgcagc tatggtaagc
    14641 ttatctaagg aaattcagga taaaatttta gaagaagcaa cagcaaaaaa tcaagatcaa
    14701 ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac aagaaacaaa attctataac
    14761 gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat tagtgccagc tactgtattt
    14821 gaacgtgtat ttgaagattt agaacaatct catccactat tgcaaaaaat tacttttgtt
    14881 aacacaactg gtgtaacaga atggattgtg tcacgtggag tcaatccagc atggtggggt
    14941 aaactttgcg aagctgttaa aaaagtttta gataatggct ttgatgtaat taacatgaag
    15001 cagttcaagc tatcaggtta tattcctgta tgtaaggcaa tgcttgattt aggtccagta
    15061 tggttagatc gttatgtccg tactgtttta gtagaatcat tgagaattgc attagaacaa
    15121 gcaattgttg atggtactgg taaagatatg ccagtcggaa tgatgcgtga catgagcaaa
    15181 caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta ttacagcttt agatgctgta
    15241 actatgggcc gtttgatggc gcgactatca aaattcaata tcgaaggtgt gaatgatccg
    15301 atttatcgta atgtgaatcc ttctgatgtg gtcctaattg tgaatccaac tgattactgg
    15361 tctaaagtat tcccagctaa gactgtacta actgctaatg gagaatacgt acaagtattg
    15421 ccagtaccag tttcagattt gcagtcaacg gctgtgccag aaggaaaagc agttattggg
    15481 gtagcctcag attacttcat gggtgtagga tctacactaa aaattgaagc ttcagatgaa
    15541 taccattttg ttgaagacga acgcatttat ctagctaaac aatatgcaaa cggtcaacct
    15601 aaacgtaacg atagtttcat tgtattagat attagcgctt tgggaactac tactacaact
    15661 acaaaaccaa caaccacaac aactacaaca caagcgtagg tgatcagaat gaagtatatt
    15721 ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg aagtttgttt aactaatctg
    15781 aagaaactag gaatcaaaga tatcgtattg cttttcagca gacacgatga tcagattcct
    15841 attttttttg agaaggaata tggtgttgaa gttcatgtgt acgatgatct gcgggacgac
    15901 aaagagtata ttccttcgat taaaccatat ttatggtgga aatatttaga agaagatcat
    15961 tcgcgtgagg acgaccgata tttctatatc gattcggatg tcattttcaa taaaagaatt
    16021 aatttgcgca aattgccttc taaagatgat gtttggtatt gtagcgactg ctgtagttat
    16081 ctaagtcttg attatattag aagctgtgaa aacggagaaa atattctaaa agatatggca
    16141 aacattgtaa atgttacagt agaatctttg gaaactataa acactaattc aggaggcgca
    16201 cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa aggtttatct ggattctaat
    16261 cggctatatc gctaccttag agggcaaaaa acaaatatac aaatctggac agccgagatg
    16321 tgggcacggc tttggaacat gatgtatttc aatattggtc ctaaagttca cgaggaatta
    16381 gacttttgtt ttgctactga tccaatagaa aaagttaaag aagtaaaaat cttacacaat
    16441 gctggagtaa caacaaacga tgaagattta tttttcaaag ggagatacgt gacttctacg
    16501 ccttttgatg aagatttatc atttgtaaac aagaaaaaat gctcttacgc atatgttaaa
    16561 gcaattaagg cggtggttag atgacgcctg aacaagtgac tgaagaattg ctaatagctg
    16621 tgaaggataa tatttacgtt acctggaacg aagaagatga gtcaattaaa aagatgatag
    16681 ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac actttctttt tctcctgaaa
    16741 gcttagaata cggattgcta atcgaaagat gtagatacga ctggaatcgt gctttagatg
    16801 agtttgaaca aaatttcgct agtgagttat taggtttcat tcaacattat gcgctacaag
    16861 aatatattgc aggtgatgtg aatggcgaat aatcgtagac tcgaagaaac attcaacgat
    16921 ggttggttaa agattttgac gcaaactacc aaaagaaatg aactaggaaa aaagattggt
    16981 gtagaagata cagaaatcac ttctttaaaa tttagaaatc tttccatgag agatagtgat
    17041 ataacagcta tggatgcgat gggatcgaaa ttaactaaga aagtaaagac tccatttcat
    17101 ccaatcgcca agaaatttaa taaagatcaa tattttatcg taatcgatag tatgcgttac
    17161 aacgttatct atgccgatta cgataatttt tatatctatt tttatcttga aagtgtgggt
    17221 gaatatggtg attgataatt ctaaagaaaa agaacgttta aataagcaaa tttctgctat
    17281 caaaacttcc ttagaagagc attttggcct caaactcttt caagactccg taggcgagga
    17341 tgagctacct gatgatttta attacttcat tctcgaaaca ggagaaatag aaatgatcac
    17401 tgagccaaaa tatagcgtgg gtcaaaatct atatctaact ttctattcag aaaatagaga
    17461 agatttaaca ggagactcac tagatattat ttcattgatt caaaatcgtt cgattcgttt
    17521 tcagagaatg gatcccaatc atttaaaact agagaaccaa gatcgctata tcgatcaatt
    17581 ggtatttacg tttagacgat tattgaagag tgattgtcat ggctaaaaat agttgggagc
    17641 taaaaataaa tggacatgat gaacttcttg tgcggatgga acgctattca agcgagagcg
    17701 aacgactgat taacgaagca ttgaaatcaa aaggttcggc tattgcagtg gataggatta
    17761 cagaaaaaat tcctgtttct gaagcagatt taagaagagg gcaccaacac gcaaaaaata
    17821 gtcgtccact taagactcaa tacattaatt tgggtttcat cattagacct acaagaaaat
    17881 ttgagtattt aaaatatcct gatttgggga taggtacttc taaaagaaat cagccggacg
    17941 aatttatgag aagaggatta ggtcttgcac ttgatccaat tacagaactt ctgattcgtc
    18001 aattcgataa attaaataaa tagggggaac aacaatggct aaaacaaaaa ctgtagtaac
    18061 aacgttcgat aacgtaagta tcaaacgaat tgcttttaat tttaagaacg caggaaatgc
    18121 aatcgcaacg aattgtaatg gacaattaga tggcgaaaca gaaatgcaaa cggtggttaa
    18181 aaaatgtgga gcgacagaag taaaatcaaa atctaaacca atcaatatga cggtaacaat
    18241 tactgcacat gtaccgatgg aagtttatcg acgtttcaat gggttgaaac aagatggacg
    18301 tattaaacca ggcatttact cttacggtcc tgattccgta ggcgaagatt tctcacttgc
    18361 tgcagagatc gtggatgact tcgaagaaaa tagaaagtta gttggtatgt tagcatgcac
    18421 ttcgaataca ggattaacat tctctattga aaatggtgcg gatgaagtag ctgcgttaga
    18481 actagaaaca aaagttatgc aagatgaatt tggtaaattt tatcatgaag caattgttgc
    18541 agaacttgaa gaagacttaa cagatcaatg gatgacgaat ctatctgctg atgtgattaa
    18601 aaaaacttca gttgtgacaa ctacggccac tcaatcacag taaaaaaaac ggaggtagcg
    18661 aaatgaacga agattactca aaaattgaac taaacgatgg aacaattttg aatttagaac
    18721 ctaaactgaa tatcaagaaa ttattgatga tcaatagaga ttttaacaca gacgagtttg
    18781 caaaaatgac tgtgggaaaa ggatccatgg atatttctgt tattcaaggt gcaaaggctg
    18841 tgtatattgc ttaccgccaa gcgaacatga ctgattatat ttcattcgat gaatttatcg
    18901 ataaatggga ttttgatatg gctactgcca gctatattta tcaattgatg atgttcaaac
    18961 aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa taaggaaaaa aagcttcaaa
    19021 agtaaaaatg ccaaagctct tagttgaaac gtgggtcgat gtctattcga tgttgaccga
    19081 cgttttttct atgccttcag atttggtttt aagcgatatc tgtttagatg acattttgca
    19141 aatggcttac aacaagagtg cttatgaagg atggaaaaac tatgcaataa accaatctca
    19201 gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag aacagaagca gaagtaactt
    19261 tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta
    19321 aaaatagagc agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag
    19381 acaagttagg aagtaaatta tcttctttag aaaagcagta tgaattacaa agccaaaaag
    19441 ttgaagtaac tagccaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg
    19501 aagttcagaa acttgagaga gaactgatta accaacaaac agcgcaacaa cgtttgtcaa
    19561 acgaaattga taaaacgagt aatacactag ctcaagcaaa aggcgaaata cagacgtacg
    19621 agtctacaat gcaacagttg gatagtgaac aaaaaaatgt tcaagctagt gcttctctga
    19681 ttgaatccga atacaaaaaa tggcaagtaa ctgctggtca atcagcttct gaatccgaga
    19741 aattagcgaa agcccaagaa tatgtttctc aacaatctga aaatgcagag aaaacgatag
    19801 atatcctgag acgacagtta gaagctacac agtctgagtt tggcgctaca tccacagaag
    19861 caatgcagat ggaggcgaag cttaatgatg ctgaacgtga atttgaagag ttaggacaag
    19921 ctgctaaaaa tgtagataca actaacttgg acgatatcgg aagcaaaata gatatgaata
    19981 atttaatgga agcttctgac gttttaagcg acattggcga taagcttaca gaattaggga
    20041 aacaagcagt ggactctgct aacagtgtag gtagttccca gagtaaaata caagctaatt
    20101 ttggtttgac taaacaagag gctgaagaat taacgaatgt agccagagac atttattata
    20161 aaggttttgg agaatcgtta gatcagtcca cagatgcatt gattttggta aagcgtaatt
    20221 taggcgattt aaataatcaa gatttacaaa atattacgga acaagctatg gtcctagaaa
    20281 acaccatggg cgctgatatg gatgaaacgt tacgtggtgt aaatggctta atggtcaact
    20341 tcggattgag cgctcaagat gcaatggatt taatggtttc gggtactcaa aacggtttag
    20401 ataaaacgca cgaattaggc gacaatatgg cagaatatag ccaattatgg agtcaaatgg
    20461 gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg tttagatgcg ggtgcttata
    20521 accttgataa agtcaatgac ttagttaagg aaatgggaat atcgttaaca gatggtcgat
    20581 ttgagcaaaa catggatatg tttagtgaaa gtactagaaa agcttttgaa gagtggaaaa
    20641 atggtggagg aacacaaaaa gacgttatta attccatgat tcaagatttt agcaatatgg
    20701 atggtcaata cgaccaatta aataaagctt ctacaatttg gtctgcactt ggcgaagata
    20761 acgcgatgaa agttgtccaa tctttgactg atgttaacca tacatttgat gatgttagtg
    20821 gatctgcaca aaaaatgaat gaagattcta ctactccgtt acaagaatta aacggaaaaa
    20881 tagctgaatt aaaggattca ttagctccta taggcaacac aatcatagat gcactcgaac
    20941 cagtaattga ttttctagga aagatggctg atgcgtttaa taatcttcca caaccagtac
    21001 aggattatgc cgtagcgatt ggcggattga ctgctgcatt tactttatta atgccaataa
    21061 tagttggctt catggctcta ggtggtccta ctacattaat aataggagca gttattactg
    21121 ctattgctgg agttatagca attataaaaa actggggcgc aattactgac tggtttaagg
    21181 gaatatggag taaattcact gattggttgg gtggtacttg ggaaagcata aaagaaggtg
    21241 cctcatcagt ttgggatgga gtcaaagaaa cctggtctgg atttgtagat tgggttcaag
    21301 aaatttggca aggagtttct gattggtttg gagagttatg gagcggatta gttgaaggag
    21361 cttccaacat ctggcaagga gtccaagaga cttggcaagc attcgttgat tgggtttcaa
    21421 atatttggaa cggagtcaaa gaagtatggt cgattatttg ggcagacatt gtaggaattg
    21481 ttcaaatacc atggacatta ataacgtcat tgattcaagc tggtattaat attatcgtgg
    21541 gtatctttga tgtagctgga cagttattag gcgcagcttg gcaagctgtt tggacaccta
    21601 tttctgattt ccttaaaaat acttgggata ctatgacaca atggataagc atcgcttgga
    21661 atggaattgt aactacattc catactatat ttgatccagt agtggcatgg tggaatggta
    21721 tatggacatc tattagtact acggcttcaa atatttggaa ctcaattagt gcaacagctt
    21781 ctagtatttg gaatagtatc aagaatacaa tcactagctt ggtacaagca gctgctacag
    21841 taattcaaaa tatttggtca actgtatcta gttggttagg tggaatttgg aattcaatca
    21901 gctctacagc atcaaatatc tggagtagcg tgactagtag tataagcaat gctataaacg
    21961 cagctaaaag tgccattcaa agtgtttgga atagtatatc ttcgtggatc agcggaattt
    22021 ggaacggtat caaaaacact gctttgaatc tttggaatgg aattacaagc actattagct
    22081 ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta acaggtattg
    22141 tttccgacat attcaataat gttaaaagca cgatagctaa tatatgggaa ggtatcaaaa
    22201 agactgttag cgctccgatt gattggatta gagataaaat cagtagtgtc tttgataatt
    22261 tgaatatttc tataccacat attccgttac cacattttaa attgagcgga gaattcaatc
    22321 cattgaaggg gaaaatccca acattgggtg ttgattggta tgcgaaaggt agtgtgttta
    22381 attctccaaa tattatcggt gtaggtgaat caggacctga agcagtttta cctttgaaaa
    22441 gatctgtgct gcaagaaatt ggtgatcgta tcttgagtag cacctcagtt tcatctagga
    22501 cacaagcggt tcaacctgtg aacaattacg aattcaattt cacaattgat ggtaacgcag
    22561 atgaagtcac tatgaagcaa acaactcaac aaatcattga tagcattaca aaagttcaaa
    22621 atgataatgc ttcggcatgg cgttaaacag gagagtattt ctcctgtttt tttagtatta
    22681 aaaaggatgt gaaaaaatga ctgattgtat acattctata atcgatggat ttcctgatta
    22741 tttgcataaa ttggctttag ctgaaagacc aaccatacct tctccaaaaa gacagagagt
    22801 tgaaacttct gttttaggaa ggttaggtgg cttagtgcaa gattactcgt ttgaagacat
    22861 gtcgtttaca ttgcactata actatttaga ggatgtggaa gaccatcaag cgttcaagca
    22921 atcgttttat atcatgcgtc attggttaaa ctatgcaaag aaattagaat tctctgacga
    22981 tcccaacgtc tattacgtta tccagactat cgatattggg gatgcagaaa acgatattgt
    23041 tgaatgggga gagttcgatg taaatattac tgcgaaacca ttcgcaagag ttcaagaaga
    23101 tgtacctata accgtagata aaccacagtc atttaacttg ctgaataata gtttagaaga
    23161 aagttttcca aagattatca tcactccttc agctacttca tgccagttca tcttaaatga
    23221 ttatgtgttt agtttcgaag gcttagtagc aggaactgat gtagtcattg atagtgattt
    23281 gatgctttgc tacgaagagc aatcggacgg agatatttta gatcggtcca acaaaatgaa
    23341 gaccatgcaa tatccgacat tgcaagtgga tatcaatcat tttaattgca ctggtttgag
    23401 caaaatacaa atttatcgta acgggttaag gtaggtgaaa tagatgatcg ataatttaat
    23461 aactatttac gataaaaatg acgcgaataa tttagctgaa catttatatg atacgcaagg
    23521 tttaggcgct ttgtcagact ggttaacagc tactgttagc aataaactaa acggagccga
    23581 gatatttcag ggtacttatc caataagcgg aactaatgca gatttgattg tagaaggacg
    23641 tattattcag tgttatgtag atgaaaatcg agcaaaacag cgtctacgga tttattatgc
    23701 aaaaacttct gtaataggca atacgataga agtaaaagct gaacctattt tcaatgatat
    23761 aagaaaatcg gtgttgaata aatatgacag cggaacagaa aaaatcactg ctactcaggc
    23821 atggcaaaac gcaaaagctt tagcgaaacc agctatccct tcgcagtttt ctttctcgtc
    23881 attagtagat acgcttgcta atgtgaagat agaaaaagcg aattttttag aattctttgg
    23941 tggaaaagag ggatctattc tagatcgatt tcatggggaa tttcttaaag ataataacac
    24001 attacatcat gaaaaaaggc taggtacgga tcataaaatc aaagcgattt atactaaaaa
    24061 tttaactggt cttgacttag agatagatgc tcaaagcgtt ttagttggag tttatccatt
    24121 cattagcagt tcttcagaag gagaagacga gatcactcta ccagaagaag tcattttcac
    24181 ggattacgtg gatgattatc ctgctggata tgtttctttt gttgatttta aagacaaagc
    24241 gactgatgta gccacattaa gggaagctgc taaagactgg ttgaaaacaa acatagataa
    24301 acaaaaacca caagtgagtg gttcgattga attagtacca ttgaggcatc aaagaggcta
    24361 tgaaaaattt gttgatctag aaaaagtttc gatgggtgac ggagtagatg tgtatcatcc
    24421 acagttaaaa gtgaatatgt cagcgagaat tgtggaatat acgtttaatg ttctaaccaa
    24481 ttcatacgat aaattagttg taggaaacgt caaaacaaac ttcttagaaa acacagagaa
    24541 taatgtaagc aatttgatta atgatgccat tgatcaattg aaaaatggtg gcgaaatcag
    24601 tgatttaatc aatgatattg tagatcatca aactgatatg attactggtc aaaatggtgg
    24661 ttatgtttta ttagatccta aagaagcgcc tagtcgtatt ttgattatgg acacaccaga
    24721 taagaatacc gcaaggaatg ttttacaaat caacaatgct ggtattggtt tttctaaaac
    24781 tggcattaat ggaacatatg aaaccgcatg gacattagat ggcggattca atgcctcgtt
    24841 tattacggct ggtgaaatag taggaattac tattagaggt actacattaa ttagtgatgg
    24901 cactgattat agaacaagta ttgctaatgg caaaatgact tggtactcaa aaaaagttaa
    24961 caaagatatt atggagctag aagcacgtga ttatgtaagt gctgatgccg gtattgtatc
    25021 atacaccatg aaaactggtg gtggtttcat gattagaaat ccacagggta acttggtttt
    25081 tagtacgtgg gataatggta ataacagacc gtttctatct tttggtgcgc ccaatttcag
    25141 gtatagcaat gctagttatg taacttctgg cgacggtagt tctttaagca ttaatggtag
    25201 tgcgggtaac tcatgggaat ttaaggtagc tggtaggact atgaaattta ctagtgatgg
    25261 tatgctaacg ttgccaggtt gtttttttgg ttcatgggaa gatgggaaac ttgctaggtt
    25321 tgaacaatca acggtacaag tatataaaga ttttactgtt agaggtacta aaaactcaac
    25381 cgtaccaaca gaacattatg gacaacgact attaaacgct tatgaaactc cagaatatta
    25441 tttcgctgat tatggggaag ccgttacagg tgatgatggt aaagttcgtg ttgatattga
    25501 ccccatgttt gctgagacag taaatctaag tcgatatatg acacatgtga cacctacaga
    25561 actagttttg tgtgctgtta ctcatgaaga tattgaccat ttcatcattg aaactagtaa
    25621 gccaaacgta ttagttagat ggaatttagt ggcacaccgt ctagggtatg aagatattag
    25681 attaaaagag gatacagcat atgatagcac agtgcttgac caaaaacgtt tttaaaacga
    25741 agacaaggag gtatataaat ggctagcagt ttatataatt tggctctaga tttcagcaaa
    25801 gaattaaact acaccaaagc tattatggct cgtcaaggtg ataaagggat tacagtgact
    25861 gttaaaccat ttttaaatgg cttgcagatg gatacgagtg gcggaacatt tactttaaaa
    25921 ggaacaacgc catctaaccg ttacgtagat agtgtggcaa ctagtgtaac tagtgaagaa
    25981 gtcacgtttt ctcttgatgg cacatttatg agtgaagcag gatattataa acactgctac
    26041 gtagaatata gaaaagacaa tcaaatttta acaacgcaag atatcatttt tttctcacta
    26101 ggagtgtctg acatttcgca aggccaagcc gatgaatacg tttcgcaatt ggaagagttg
    26161 attcaaaagt ataacgaaac ttttgatgct tttatggctg aaatcgaagg tagagtggac
    26221 agcttaaata aacagattac tgatttaact ggtcaagcta aaacactaca agacaagtta
    26281 gacgctctga aagaagaaat ttctaaatta ggtaacttgc aggtgatgta cagtaacagc
    26341 atcgacttcg ggggatatga ttatagcggt aagccgaatt taatgtccaa actaaaatcg
    26401 agcgatttta acgttggtta ccacgggtca ctaacttcgg ataacgaaaa gctacatttt
    26461 acttctgatg gtacaggaag cattattatg tttacgcgta ttaatacacc tcagcttgct
    26521 agtgggaaaa cctatactct gagtgcgaaa gttcgatttg atgaaggaac tacaggagct
    26581 attgataaat tacgtttggt gtatcgtaca tcaccaggag aaaagatatt attggaagca
    26641 aatagtacaa atattacaac agatgatgta gggaaagaaa taacaatcaa aggtacagct
    26701 aacgttaatt atcaaatcac aaatttagat cgattttata tgagtattag ctttgttgac
    26761 agggataaaa taaatggcgg atttaagttg tacgacatca aaatcgaaga aggctcaac
//
LOCUS       DACDSR010000030        26461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDSR010000030 DACDSR010000000
VERSION     DACDSR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.030"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..532
                     /locus_tag="IWK15_001316"
     CDS             134..532
                     /locus_tag="IWK15_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9546914.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            516..1211
                     /locus_tag="IWK15_001317"
     CDS             516..1211
                     /locus_tag="IWK15_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9546915.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1795..2682)
                     /locus_tag="IWK15_001318"
     CDS             complement(1795..2682)
                     /locus_tag="IWK15_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9546916.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2867..3706
                     /locus_tag="IWK15_001319"
     CDS             2867..3706
                     /locus_tag="IWK15_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9546917.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3902..4876
                     /gene="sgrA"
                     /locus_tag="IWK15_001320"
     CDS             3902..4876
                     /gene="sgrA"
                     /locus_tag="IWK15_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9546918.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5174..5530
                     /locus_tag="IWK15_001321"
     CDS             5174..5530
                     /locus_tag="IWK15_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546919.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5533..6690
                     /locus_tag="IWK15_001322"
     CDS             5533..6690
                     /locus_tag="IWK15_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546920.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6759..8099)
                     /locus_tag="IWK15_001323"
     CDS             complement(6759..8099)
                     /locus_tag="IWK15_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9546921.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8258..8698)
                     /locus_tag="IWK15_001324"
     CDS             complement(8258..8698)
                     /locus_tag="IWK15_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9546922.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            8858..9364
                     /locus_tag="IWK15_001325"
     CDS             8858..9364
                     /locus_tag="IWK15_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9546923.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9532..11445
                     /locus_tag="IWK15_001326"
     CDS             9532..11445
                     /locus_tag="IWK15_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9546924.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            11450..12451
                     /locus_tag="IWK15_001327"
     CDS             11450..12451
                     /locus_tag="IWK15_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9546925.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(12653..12859)
                     /locus_tag="IWK15_001328"
     CDS             complement(12653..12859)
                     /locus_tag="IWK15_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9546926.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(13505..14353)
                     /locus_tag="IWK15_001329"
     CDS             complement(13505..14353)
                     /locus_tag="IWK15_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9546927.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            14517..15419
                     /gene="gnd"
                     /locus_tag="IWK15_001330"
     CDS             14517..15419
                     /gene="gnd"
                     /locus_tag="IWK15_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9546928.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            15421..16422
                     /locus_tag="IWK15_001331"
     CDS             15421..16422
                     /locus_tag="IWK15_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9546929.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            16419..16703
                     /locus_tag="IWK15_001332"
     CDS             16419..16703
                     /locus_tag="IWK15_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9546930.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            16736..17533
                     /locus_tag="IWK15_001333"
     CDS             16736..17533
                     /locus_tag="IWK15_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9546931.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            17547..18380
                     /locus_tag="IWK15_001334"
     CDS             17547..18380
                     /locus_tag="IWK15_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9546932.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            18398..19255
                     /locus_tag="IWK15_001335"
     CDS             18398..19255
                     /locus_tag="IWK15_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9546933.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            19277..19786
                     /locus_tag="IWK15_001336"
     CDS             19277..19786
                     /locus_tag="IWK15_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9546934.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            19823..20224
                     /locus_tag="IWK15_001337"
     CDS             19823..20224
                     /locus_tag="IWK15_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546935.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            20336..20689
                     /locus_tag="IWK15_001338"
     CDS             20336..20689
                     /locus_tag="IWK15_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546936.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            20723..21166
                     /locus_tag="IWK15_001339"
     CDS             20723..21166
                     /locus_tag="IWK15_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9546937.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            21183..22268
                     /gene="uxuA"
                     /locus_tag="IWK15_001340"
     CDS             21183..22268
                     /gene="uxuA"
                     /locus_tag="IWK15_001340"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9546938.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            22261..22974
                     /locus_tag="IWK15_001341"
     CDS             22261..22974
                     /locus_tag="IWK15_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9546939.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            23726..23926
                     /locus_tag="IWK15_001342"
     CDS             23726..23926
                     /locus_tag="IWK15_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9546940.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24494..25057
                     /locus_tag="IWK15_001343"
     CDS             24494..25057
                     /locus_tag="IWK15_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546941.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(25118..25483)
                     /locus_tag="IWK15_001344"
     CDS             complement(25118..25483)
                     /locus_tag="IWK15_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546942.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(25559..25804)
                     /locus_tag="IWK15_001345"
     CDS             complement(25559..25804)
                     /locus_tag="IWK15_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9546943.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25874..26128)
                     /locus_tag="IWK15_001346"
     CDS             complement(25874..26128)
                     /locus_tag="IWK15_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9546944.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT         9063 a         4135 c         5431 g         7832 t
ORIGIN      
        1 cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca
       61 ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg
      121 aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta tagaagcaga
      181 agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg aaacattaac
      241 agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt gtgatatgaa
      301 gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg tccaaactaa
      361 tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc cggaacttcc
      421 aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga caatcattaa
      481 aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt gatacagcaa
      541 atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta acaacaaatc
      601 ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa gaaatcaaaa
      661 aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa gaagaaatga
      721 tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc aaggtgccgg
      781 tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat ttgattaccg
      841 gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga gcagattata
      901 ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa gtgattgatt
      961 cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca gctagtttca
     1021 aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata acgttaggta
     1081 aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc
     1141 aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg
     1201 attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag
     1261 gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac ttatttctca
     1321 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1381 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1441 ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct tttttaaaat
     1501 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1561 aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga tagaaataaa
     1621 aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt tttggtacaa
     1681 ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa taagaaggaa
     1741 tttccaaaaa tcacttctca aattttgcca catccccaat tgggtcaaat ttgtttattt
     1801 gaatatattt tttacataag gatctttaat agttacattg tcttttttca tcactttacg
     1861 gattgtttta tccatgaatt ccgtatcaag agtttttgct tcggtagcaa ttttttctag
     1921 ctcagatttg tatttgtctt tattagaacc tttatcctgt ttttttacca tttttactag
     1981 ataatagctt tgctgataag tcgttggatc tgttacagga atgatatcag aaacttcccc
     2041 gttttttagt ttaaatgctg ctttttttac ttcagtagga atctctggat tggttgaatc
     2101 aaaattcatt tttccatcag tttcctttaa tgttttattt tttccataag cttgaacaag
     2161 cttggagaaa ttttcccctt catttgcttc tttttttgct ttatctgctt cttctttagt
     2221 cgaaaataga atgatctgtg tactaacttc tggatagaat tctttccaag cagtgtctag
     2281 atctttatct gttaatttta cattggcttt caaaccgtat tggaaagcca gcatctgttt
     2341 aaagagtttc ttatatgtct cttctgtttg gccaactgct tttaattgtt ccttgtagtt
     2401 tggaccgagt tttttgattt gttcattata ttcttttgtt acttcctttg aagaaatctc
     2461 ttttccgtat acatcatcaa atgctttgaa gacaatcatt ttttttaata gtgtttgatt
     2521 atttgattta acttttgggt tatcataaaa atcctgaact gtaagtgttc cacctttaaa
     2581 cgttgcaatc gtttcattgg acttgctaca acctgagaat agtaaaattg cacatgctga
     2641 aaggcacata attagtagtt gttttttcaa aaaatttccc atcctttcct tattaaaaac
     2701 agtctaacag aagaattagg gagacagtct ttcattttcg taaggttgct gtagatattc
     2761 ttagttcgta aaaatctatt ctctctatga tggagaacat ttacatagtt ttcacgagac
     2821 aagaaagaaa agggttgtta aacttaatat aaaggaggga tgaaagatga tcaggattat
     2881 tcggttgatt ggatctgttt taatagtctt agttggtttt gttttaggaa ttcttgtcaa
     2941 taatgcactt gcggatatgt ttattggaaa tagtgaatgg attggagcaa ttgccggtac
     3001 ggtcggacat ttagctgttt ttttactagc tttatttctt gctagtaagg tcgaaggaaa
     3061 aagaatagaa gattatggtg tttctgttcg aggaaaagac tgggggtatt tagcgggagg
     3121 tctcgttgtt ggaattggag tgtttctgct tattactagt ccattatatt tgacgggggc
     3181 gtatcggtta gattcaggaa acgcaaacat tgtgccgctg ataacgagtt ttattttatt
     3241 tattgcagtg ggggcatcag aagagctttt attccgtggc ttttttcagc atcagatgct
     3301 tagatttggt ccgttaatag caatgattgg atctgctgca ttatttgctc ttttgcacgg
     3361 attgaatcct aacatgacat ttctggcagt tttcaatatc tttttagcag gttgcttttt
     3421 cagcgcactt atctacagta caggaagttt atttacggct attggtgctc atattacttg
     3481 gaattggaca caaggagcta ttttgggtat accagtcagc ggaacgcaag aacaaggttt
     3541 tttctccact ctgatccaat cgcaaaatac aataattact gggggaaact ttggggctga
     3601 agcatctata tcaactacat tattcttgtt agtcttgatt gttggtttgt tactctatgc
     3661 atataaaaca aagcggatga agaaatacac tgctgtttat caataaaaga tgaagaaagc
     3721 aaggctgata tcggtcttgc ttttttaagt ctgtcattga ttaaacgaaa aagtaaacaa
     3781 attcaagcga atacacgaca tcttttgtta gattttcaca actgactgtc gtgttctcat
     3841 cattttcatt ttatataatt cctttatcaa atagaaggta attatatgga gagtgataga
     3901 aatgaagaaa acagcgaccg tattatttgt tattcttggg ctgagtataa ttgcagcaat
     3961 gattggaatc agtacttctg caaatgaacg ggcaaatgag aatctttcat ttactgtgaa
     4021 aactgatcgg attgtttatg acatgacaca acaagtcatt actattccag tcaaaccaaa
     4081 caaaagcgtg aatgctagtg atgtacatgc tgttctaaca tatggttggg atggtaatgg
     4141 aagttcagaa aaagttattg gggaagttta tttaaaagac gtacaatgga ccgctggtat
     4201 tgagtataca ataatgattt ctgcagaact atcgattgat gagatcaaga gtaaagataa
     4261 agttgatttg attgtttttt atgatggaca gatgacgatc acggagaatt tgaaaccaag
     4321 cagttggaca gttgttggac cgccaagcag tacggatagc acaagtagtg aaagcagtac
     4381 agaaaacacg agcggtgaaa gcagtacaga gagtacgagc agtgaaagta gcacagaaaa
     4441 cacgagcagc gaaagcagta cggagagtac gagtagtgaa agtagcacag gaagcacgag
     4501 cagcgaaagc agtacggaga gtacgagtag tgaaagtagc acaggaagca tgagtagtga
     4561 gagtagcaca ggaagcacga gcagcgaaag cagcacagga aaagtaaata atgaacttga
     4621 tttggtacct atcataccaa ctaaggaaca aaagagaaat gcagggagca acctaaaaag
     4681 tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat catcaagaaa aagcgaaaga
     4741 aacagataat agaaaaagcg atttaccaga aactggtagt agaaccttga acaagctaat
     4801 tacatggatg ggtgtgttac tcattttgat tgtaggagca agctattttc gttctctctt
     4861 ccatcgagtg aaataaagaa taaaagcaac agatctaaaa ttgaataaaa cgatagattt
     4921 ttgagtgaat atttcattcg agagtgctgt attgcctggc gaagttgtag ctttgccaga
     4981 tctatttttg tttactaaca tatttgagaa acaggaaata acaggaacac aaatggaaaa
     5041 taagagcaag aataattgac aatgcacact gcgcatggta gtatctctga aaaatcagta
     5101 acgaatctat ttgtacgtta ttcatgtagc tgtcggaata agtctaaata atagataaaa
     5161 aggagtatgt actttgtttg aagaatttat tgatatcaat gaaaggcaag tatatcaatt
     5221 tttgaactat tgttatgaaa gagacgagaa attatatgtg gtgaaggata tcgctttaga
     5281 tctaaactac accctggcta agatgaattc ggtgattcaa caagcagaat cgttttgcga
     5341 aaggtatcca gaatataaat tatcttttct ttcagaaaac aagatgataa aagtcgagtt
     5401 ttcttctcag tttttactga gtaaagttta ttctatactt ttagagggaa cgattggtta
     5461 tattttactg gattcacttt acaaaggtac ttatcaatcc ttagaaaatc ttagtcaaaa
     5521 aattatataa gtttgcgtac gattcaaaga aaattaagag aacttaatac tattttgaaa
     5581 aactatcatt tatcttgcag cttgaaaaaa ggtaatccga tagaaggaga agaataccgt
     5641 atccgatact tttttcactt gatgtattgg cagtcattcg acgaaaatca ttctgatata
     5701 ttatcaagta taaaaaataa acgtcaacta ttagaaaaat ttgcgaaaca tgcgccatat
     5761 acgcgtagta tagatagaaa gaaatggatc agcctactaa gaatttcgtt gcaacgaatg
     5821 aaacaaggcc actcgattac gtatgtccca gaagagatga acaaatttgt gcatccattg
     5881 ataacaaaag aagagtttac gcattacata ctagagcctt tttttgaagc aaacttctgg
     5941 tcgatgaatc acatcactcc aacagagcaa gcttaccttt attttatgtt tggtgtaatg
     6001 aacacctatt tggaagaaga tttgttggat actccagata aagtacaaga aagttggggc
     6061 tggattgaat cgtctattgt agaaaatttt gaaaaaaact tcaagatttg ttttacaact
     6121 tcagaaaaaa attatctata tgtcaatttg gcaatgatcc atttgtattc aagggttttt
     6181 gggacaaaaa agaaggtgga tgcttttgga aaatcagcag aagacagaga ttttcagcat
     6241 atatttccag tgatgtatcc aatcatgcaa cagtttttta gtggggtagc tcgcagaatt
     6301 ccagagctta atacttatta tgaaaagaac agacggttga tttttcaata ctgtatgtta
     6361 gtcagggata tttttataaa acacgaacaa cctgtcatat tctatattca atcaaagtat
     6421 ggtaaaacac aggaattatg ggcaaagaaa aggatattat ctatgactga acgcccaata
     6481 aaatatagtg catccgctga tcagttacct gatattgtca ttagcgatta tcctatcgac
     6541 aaaaaggcat atgatcaaac gaatacacag atattttact ggaatggaca acctacaaaa
     6601 aatgattggg cgcgtctgaa agctgaaatc ataaaaatca ggaatgagaa aaatagatcc
     6661 atattggaaa aattggattt cgttaattaa gaaaagcggc tcttcgtcaa tgagtgttgg
     6721 tgaagaaatt caatagaata atcgagatac tcacacggct agacagctat cttgtctagc
     6781 cgtgttgttg ttctggcttt agtcagttgg gtagtgttct tagttttctt gaaatatttt
     6841 cctcgatata aaaagattcg ttctcggtag ttttgaaagt ttctaaagcc ataggcaatt
     6901 cgttttagta ctttgatatg gttgttcaaa cattctatag ggccgttgga atagggaact
     6961 cgcaacgctc gtttgatttc tgttcgatat ttcttgaagg tcgtgaacac tggttgatag
     7021 tgctctggca actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt
     7081 cttctgatag cggaaagaaa aacctggtag acttcgtagc ctcgctttaa ggagtcatca
     7141 taatataata gccgatcgac tatctccgct tctgtcagat gatctcgaaa tgacggacgc
     7201 cagtagcgat gctgaccatt caattcccac gcattctttt gaagtagtct ccagtatttc
     7261 tttaagtgct tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg
     7321 ttggtaaagg cccgaccgat gtgttggcta atatgaaagc gatcggcgac gattcgggca
     7381 ttcggaaaac actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga
     7441 cgtacttgtt cacggacttt tctagggtag cgcaagaaat agtcgcgtaa aggattgagt
     7501 tgccgatttt ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc
     7561 atggctcccg agacgttttt caccgatttg aattcatcga aacacaccac ttctggtaac
     7621 ggctggttca aagaaacggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc
     7681 ggagaaatgt gcttcatacg tgcaatcaaa gacatagaaa taggctcact taatagctca
     7741 agaatggctt gtttgactct gcgggcaatg gagcagcgac gatcgctaac tgaatcttct
     7801 gctaaaaaag tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcacaatg
     7861 gtcttgtagt tactgacatc attcaataag atggatactt ttttccagcc ccatttaatg
     7921 atcgtttgct tattttgata gtggcaatgg aagcattcct ctggttgata ggtcaatgtg
     7981 ccatcaaaaa caaaacatgt ttcgtcatta atgcgctctt tgcgaaaggc atcttcatga
     8041 aaggtaagat ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc
     8101 ttgaaacact cctttgaatg gttttcgtcg actttattct aaaggatttc aaggtgtctg
     8161 tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat
     8221 attatagatc cagaaaagca gattgtttat gccaaaatta agacattgca aaaagattat
     8281 tttctttaaa atcgacaaga tgaccagaaa gcaagagttc gttgagctct tgctttcttt
     8341 tagttttaga aaggaacatc ctgattttat cttcgctaaa tccagtcaaa agttttgcag
     8401 attggtcgta gataatcggt tttttcaata aagaagggga tgtacgaatc tcaatatata
     8461 gttcactcaa actgagttca tctattctgt gaacaagatt tttataacta atagaatgag
     8521 tagagagcat ttcggtcacg ccgttgtttg tcaattttag taagtggata aattcatttt
     8581 tggttagtgg ttgacgaatt aagttctgtt cgataaacgg tatatcatgg acgataagcc
     8641 aacgttttgc gcgtcttgaa gacgagcagc cagaacctgt atacaattta atcatcatac
     8701 attcctccaa tagctaagta tttatgaaat aataacatga agaaatagtc gaaaagtgcc
     8761 atttagaggt gtaaaataag aaaatgttgt cacgtataat ggaactagct agataagaaa
     8821 tactattaaa tcaagaaaaa agaaagaagg cacaagtatg tcaaacacag ccgacgcaat
     8881 agtagtgatc gatttacaaa aaggggtatg ttttgattct tcgccgatac acgaattaga
     8941 aacagtgaca gcattagtga ataaaagaat caaaacatac gaaacgattg ggaaaccagt
     9001 tattttcgtt caacacgaag atgctcaatt agtgccacaa acaaaaccat gggagctatt
     9061 gtcggaatta tctattcctg agcacagtta ttttgtgaga aaaacccatg cgaattcatt
     9121 ttataagact aatttgagtg ttttattaac tgaattggac tgtcattcga tcgaattatg
     9181 tggggcacaa gcagagtatt gtatagatac gactgtgaag tttgctcatg gattaggata
     9241 ccagcttatg atgtgtagag gagcaacgac aacttatgat aatcctttta tgaaagcaaa
     9301 ggaaacaatc gctttttacg agaaaatttg ggataaacga tttctgacat ttttggagca
     9361 ataagttgtt tcttcgttta aaaaaagcag aatggatact ggtttatcca ttctgcttta
     9421 ttacgtaaaa tgagaaaata atcctgttct atttttatgg gataaaccat ttattttttg
     9481 tataaaaaat gtttgcgttt acatataatt ctcctagaaa cataaaaagg agtgaatagc
     9541 ttgagaacaa aacaagtatt aaagggagcg ttggtcatct tatttgctgt aagtacaagc
     9601 gtcccgcaat ggacagtata tggagagagc atttcttccg aatcggaatc tcgggaggta
     9661 gaaaccacag aaactactga tgctggaaat gaaacgaaat cgagtacttc acaaacagaa
     9721 acaaatactg aaacaacggt tgagacatct gttactagca cagatcaaga aacgaccgct
     9781 accacagata accaacaaac agaagaagca acagctacaa aaatgtcaaa cgacgaatcc
     9841 tcttctgcta aagtaagtga caaagagaat gggacagaaa atgatgatat cggtaaaggt
     9901 gaaaatttca atggtaaaga atatttgaat tacttcggta aacagatgac agaaatactt
     9961 aatggaccaa cagaagacat caagtcaaaa ttaatagagt tattagtcac tgagcttgct
    10021 gttaatgatt atggtgtaga taaagagatt atcaaacatc tgattgctac ttatctattg
    10081 tacgatatcg cttttgcaaa aaacagtgtg ttgatgtcga tgcttggagg tacagatgat
    10141 gggacaacgc ctacagaaaa cacaggcttc tatccaaata caaaacaact tcatcaaact
    10201 gtactaaaca aacaagatgg caaaatgatg gatgtatatg catattatgt cgatcaaggt
    10261 tcagataaaa cagtcatgat ccatggcgga tttcgaggga attggaataa cgggatcgtt
    10321 acagaagaat acaatgattt ttataaggcc gggtataatt tattatttgt tgattccaga
    10381 gcgactggta atagcggagg cgattatgtg acctatggac aatatgagtc agatgacgtc
    10441 ctttactgga taaatcaaga agttcgagag cggccatctc aaaagatttt actttatggt
    10501 ggttcgatgg gagcagctac gatgatgtct gtattagcga aagatattcc tgtcaatgtc
    10561 aaaggaatta ttgaaaattg cggatttgca tcaattgatg aacaactacg ttttacgtac
    10621 agccagacag tagcccccgc actaccggat gccataaaaa atcagttaga catcattggg
    10681 gatcaagagc atgaagattt atttatgggc cttttgaaac aatactattt tgatcaggag
    10741 atgcatttgg atacaaaagc tgctctacct acgattggaa tgtcagattc attgcctaaa
    10801 ttgattatcc atggaacagc agatgatgta gtacctgtgt ctaacgcgca aaaattatat
    10861 gagttatcag gagggtataa agatctttta ttagtagaag gagcagggca tggaaaggca
    10921 caggaagttg accatgctgc ctacacgaaa catgtaacgg actttttgaa agtagtgttc
    10981 catgatcaga taaacgtaaa gtatgtggat gaaaacaata aaagtctact caaagaccag
    11041 gacgaaatcc agctttacgg agcttatgga gaaaattata tgacagagca aaagacattc
    11101 gaaggatacg aattagcaaa tgttgaagga ccgactgaag ggatttttaa tgagacgata
    11161 ccaactatta ttttctatta caaaaaaatc ccagtagctc caccaaaaaa gcaagatccg
    11221 acagagaatg cagacaataa gggaaagaat gaacctcgag aggatcaatc aagtgatgat
    11281 acgcaaccga ttcagatgac agtgaaagta caaaaaaaga cagatgcaaa aaaaagaatg
    11341 ttgccgaaaa ctggagaaaa gaaacattcg cttctcattt attcaggtgg atttttatct
    11401 gttagcacga ttatcacatg gatttggcga agaaagagag ggtaaatgaa tgaaatggtt
    11461 caagcgaata gtgattgcag taccagttat catcgtagtt attttagtca ctgtattttt
    11521 gattaatcaa atcacaccaa agccgattag cttgcttgtt agaaaacaat ttgacacaag
    11581 tgaaaaagaa caagtatatg cccgaccaga tgatttccag caaaaaatcg aacaaatcga
    11641 tataaaaaaa gaaatatcct atccttcaaa gtacaaaaat aataatatgg atatctacac
    11701 accgaaagtg gaaaagaaaa aattaccgat tcttttctgg atgcatggag gcgcatatgt
    11761 aggaggggat aaaaatgatt gtcgtgatta tttggaatat ctatgttcag acacgcagca
    11821 aatcatcgta aatatagatt atgaacgatc accggaagca aaacatccta caccagttat
    11881 ccaattgaac gaagccattc aagcagctaa aaaagagata aaagatcaag cagattggtc
    11941 gaatatttcg attggtggaa attcggcagg tgcacaaatt agtggagaat atttgttagc
    12001 acttcaaaat gaagaaatta aaaaagctga tcatcttgat ccgactttgg agaacaagca
    12061 gattacgaag tttatttctt tgtctggtct gttggatcct tctgcactta ctcaagtaag
    12121 tgataaaatc tctagctttc tttatgaaaa atgtggatgg gcttactttg ataataagga
    12181 ctttgaacaa tcagagaagg tcaaaagttt agccttggtc agacatgcgg atcgttggac
    12241 gcaaaacaca ttcttaacgg atggaaatac aaatacgttt acgaaacaaa tggaagaagt
    12301 aacttctgta tttgagaaag cgggggtgaa agtcacaaaa gtagattatg cggagaaaaa
    12361 tgctgttttg aaccatgaat atcagtttga tttcagcaat aaacaagcgc aagaaacata
    12421 tcaaaagtta gtaatgtttt tcaaggagta aagtcttatg atgcaaaaga ggttgtaatc
    12481 tgtcacttag ctccaagtca caaggagcaa tttcaataaa caagcttttc atggtttatg
    12541 aatattgtaa cttgtgactg aggagttaga gatggcaagg ggcacctttt attcgaaatt
    12601 tgggaatatc acacgattat tgtcacgatc tcttttacgt aaattttata agtcagattt
    12661 gttgttttga tcgtttaaaa aaagcaacaa atccgaagcc tgcggaggca agaagggaca
    12721 aaaagccgag tggtaagcaa aagaatgctg gttccactaa tattccgttg ttattgatat
    12781 aaggctggga acaaagatga atagcaaata aaatcaatgc actagcgaaa aaaagcaaag
    12841 aaatatggat agctttcata agcagcacct ccttttttat aattgtatga taaatagatt
    12901 aagaatctat aaatgtgaca agtttgttag gatgtgaata aaatgatcat ctagcgaact
    12961 tgaaacaaag atggctaatg ataagcaatt aatgattcag caatcagata ggtacaaagt
    13021 ttaccatcat acgtctagac caataataga aaatatggga atataataga aaagatttag
    13081 gataccccca aaagaatttc cccaaaaatt cgatgtatcc taaacttttt tattttacat
    13141 aacaatcata tcaactgtga aataaaatta caaatgataa cctaaaatga ggccaaaagc
    13201 cagtgttatc aaggaaatac ttaattcacc aaaatttttt ttaatttttt agaaaataag
    13261 aaacaaaaat aataaaaaaa agaaaagata aattttttac tactgtatac taactttgtt
    13321 atcgcatcta acaagtaact cgaataatac caatacaacc ttctccaaag atgcgatgac
    13381 atacgttcta acccaccgac tattgatgtt ctgtaccacc cttgcagaac caaaaaagcc
    13441 actaccaaat atttggtagt ggcttttttt gttttatgga tttttcgctt tttctgttat
    13501 ggtgctattc tttgatttca gaaatagtat gtcgaacttt agcgatagaa cgacgtgatt
    13561 ctttttcgtt gtaaaacatc aaaatcacat atagagagtc caaaatgctt agttgagaaa
    13621 tacgggaagc taaagcttca gatcggaatt ctgtttcttc agaaatagaa ataaaaacga
    13681 catcgccaag tttagcaagg gaagaattag cttggctagt aataacgatt gtttttgctc
    13741 cagtttttct tacggtttca gcaatatgga ttgtttcttt tgattgccct gtgtgagaaa
    13801 tcaaaacagc acagtctttt tccgtcaata aagaagcttc catcaattga atatggtaat
    13861 ccgtgctgtg agaagttgcg ataggggaac gcaaaaattt atgataagcg tcagttgcta
    13921 caatctctga accgccaaca ccgaaaaaat agacacgttt agattcattg ataatagtag
    13981 ccgctttttt tagatccttt tgttgtaaca acttttttgt gtcatttaaa gttgaaatac
    14041 tagactcgaa caccttttgc gccatagtga gctcagtatc tgttttctga atattttcgt
    14101 ggatagaaat agcagataga tcattttctt ggatcaacat ggccatttta aaatctttaa
    14161 atccattata accaagtttt tttgtaaatt gaaaaaaagt agagtcagca atgcccagtt
    14221 cacttgctaa attgctaata ggactatgac ttactttgct tggattttct aaaatataat
    14281 cggcgatagc ctgttctttt gggctgaagt cgttgtacat tgttttgatt tgtagtctta
    14341 tggagacgct catgagaatt cctcattttc ataataagat tatcattttt tccgttctca
    14401 ttaatgttaa agatacaagg agagtaggaa aaaatcaaac aaaaaatatt tttgtaaaat
    14461 ctatttacaa aaaaattttt ttgtataatg agggtataag aaatggaggc gctatcatgg
    14521 aaattggtgt aattggtttg ggaaaaatgg gattgaattt agctttaaac ttaaaagagc
    14581 accatcacaa agtacaagga ctcgacattt caagtacagc tgttagtcag gcaagagaaa
    14641 atgacattcc tgcttatcaa gagatctctg aatttctagc tacttttgag cagaaaaaaa
    14701 tcatctggtt aatgcttcct gctggagaga taaccgaaaa catgttaaga aaactttttc
    14761 caaaactcgg accaggagac attattattg aaggcggaaa ctcttattat aaagattcaa
    14821 tccgtcgagc agaagagttt cagaaaaatg aaatcggtta cttggattgt gggacttctg
    14881 gaggaatcga aggagcacga catcaagcct gcttaatgat tggaggagat aaagaaactt
    14941 ttctatccgt ggaacaatta ttcaaagatg tagcaattga aagaggatat ctgtatgctg
    15001 gtgcatccgg aagcggacac ttcttgaaaa tgatccataa tggaatcgaa tatggaatga
    15061 tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag tgagtatgct tttgacttgg
    15121 aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg cggctggctg atggaaatca
    15181 tggaagatca gttaaacgaa catcccaatt taagtaatta tcggggaatc gtttctgcat
    15241 caggtgaagc aaaatggacg gttgaaacag cgctagaaat ggctgttcca gttccagtaa
    15301 ttgcaactag tttgttcatg aggaatcttt cacaggaaga agatagtttt tcagcaaaag
    15361 tagtttcagc actaagaaat gggtttggcg gacatgaaat agttgaaaag ggtgagtaaa
    15421 atgccagatt atcatatagc aatcgttaat tccagcagtt tcggaagagt ctttccacac
    15481 cacttggaaa gattaaagtc agttggagac gttcatttct ttactgttga tcaagagatt
    15541 ccaggaaaag aactagcgga gttacttaaa ggattcaata tcattattgc aagtgttacg
    15601 ccattttttc gacgagattt ttttgaaaac aaagatgaac tgctattgat ttcccgacat
    15661 ggtattggtt acaacaatat tgatttaaaa gcagcaaaag aacaccagac aatcgtatct
    15721 atcatacctg ctttagttga acgaaatgct gtagcagaaa ataatataac taatttgttg
    15781 tccatgcttc gacgcacgac acaagcacaa gcaagagtca aggacgatcg atgggaagat
    15841 cgggcggagt tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat tggtgcagga
    15901 aataccggaa gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga tgttctagct
    15961 tatgatcctt acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa agtagaactt
    16021 caagaattac tggaacaatc agatatcatt tgtttatgtg cgaatttgac agaagagaac
    16081 tatcacatga tttctgataa agaagtcgat cagatgaaag aaaatgttta tctatctaat
    16141 agtgcaagag gcgctctagt tgatgaagaa gcgatgatta atggtctgaa aagcggaaag
    16201 atcgcaggat atgccacaga tgttttagaa gaagaaccgg gacgtcaaga tcatccgtat
    16261 ttagcgtttg aacaagtagt gattacgcca catatatcgg cctacacaag ggaatgttta
    16321 gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca ccttcttcct
    16381 gaacgttctg tccagacaaa gagcaactat ataaaaggat gatgaagaaa atggtgaacg
    16441 gtttaaaagt gaaaacaggc cctcaatttt atttatatga agaaggtggt atttcaaaag
    16501 tttctgatct cttgaaatca tacggcgcga aaagagtact ggtcacccac ggaacggtat
    16561 catgggaaaa ggcgctacct aaattagtat ttttgaatga cgaaacgatt caattttttt
    16621 atcatcgtta ttctggagaa tgtagttatg ctgaagccag acgaatagct acaatcatca
    16681 aaaaaatgaa atcgatttcg tagttggcgt tggcggagga aaactgaccg atctagtggg
    16741 ttatagtgcc aatcttgcaa gaatcgattt cggagtcatc cctactttag ctagtaattg
    16801 tgctccatgg acacctcttg ctgtcatgta taaggagaat ggacaagcag aagggaaaac
    16861 agaacacttt tacagacaag ctgctttttt tataacagat ccccaattag tgattgattc
    16921 ccctgttcca tatttcattg ccgggttagc agatacacta gcaaaatggt atgaatctga
    16981 gctcatctta gaacaagatt ttttgcaaga tgaatcattt ttgaagctag cacaagatac
    17041 tgcgaagatc tgcaaagaag agattttgaa tcattctgct aaagcaatcc aagatatggc
    17101 acaaagaaaa ctgacatcgg agttcaaaca gctttcagag attatctttg ctgttgctgg
    17161 tttagttgga ggattaggag ataaatacgc aagaaatgtg gcagcacatg ctatgcatga
    17221 cgctatcagt aaatatattc cggaaagcca tcgttttcta catggagaaa aagtagcgta
    17281 tgggattttc tatcaactgg cactagaagg tcgctgggaa aaaatagacg agttatatcc
    17341 tttgtatcgg gaattagaac tgccggtttc tctagaacaa atgggattat ttccgaaaga
    17401 taaacagata atagataaaa tggctgaatt tattgattcg aaagagaaag tgcatttgat
    17461 tcctatagag gtaacacaga aatcactgat acagtcaatg atggaactgg aaggttatat
    17521 cagaaagatc tgaaggagga ggacacatgg aaacattaag tgtagtacaa agtttgttga
    17581 ttgctttatg ggtagccgcg gttatgtccc gctggttagg tggaggagcc acgttgacct
    17641 tacgtttttc tcctttgatg actggattag tcgtcgggtt gatcatgggg gaagttgcac
    17701 aagcgatgat tgttacagcc gctttgcaaa tgatttatat gggggttttt tctccaggag
    17761 gatcaatgcc agctgaacca tctattgcag cagctatcgc agtaccagtc gcattattag
    17821 gggatctgtc gccagaagca gcgattgccg ttgcggtgcc agtcggattg ctcggaagtt
    17881 acttgtatca gtttcgtttt ttcatcaata cttttttagg taaatataca gacaaagctg
    17941 tagaagattt gaatacaaaa gctatggccc gatcaattat tctttatcca acggttgcct
    18001 catttttatt attcgttccg cttgttttca ttgctttata tttaggggcg ccattgatcg
    18061 ctgatgtaat caaagcttta gagggaacag tcgttatcca cattcttgaa gtagtaggtg
    18121 gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga aaagattatc
    18181 tggtcttttt cttccttgct tattttatga gtgttgtttt gaaatcttta gatattacga
    18241 tggttactta tgccattttt gggattatta tcgctttgat ctttgtccag tcgcaaaaag
    18301 ggaaacaagt acagcctgcc acaagcaata acaattcagc atcttttgat gacgaagatg
    18361 actatgatga cggattttaa tttaggagga atgggaaatg gctgaaataa tggataatca
    18421 aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc tgagatggca
    18481 ttttgcaaac gaaattcctc actcttttga acgttacttg gcgccatcac tactatacgg
    18541 aatgatgcca atcttgaaaa aactttataa agatgaagac tctttaaaag cagcatataa
    18601 acggcagtta ctgttcttca acactcaatt aagctggggt ggcggtgtga ttactggcct
    18661 aatgtcttct atggaacagc aaagagcaga agaagagtat gaagaaaaag agatcttgat
    18721 gcaggatgac ttgatgtata acacgaaagc gggattgatg ggggcactag caggaatcgg
    18781 agatgcaatc gattcaggaa ccgtccaata tatttttatc gcaatcgcgg taccttgggc
    18841 acaacaggga agtcctttag gcgcactttt tccttttatc tgctttgggt tgtatcaggt
    18901 tttactaggg gtatttttcg caagacaagc tttcactatg ggccgtaatg ctacaggcct
    18961 aaagcaaaac actggtgttc agacagcgat tgaaatgcta tcagttttag ggttgtttat
    19021 gatgggggtg cttgcgggaa actatgtaaa agtagaatct tcattgaaat ttgcgatatc
    19081 cggacgtgag tttgttatcc aggaaattct tgatcaaatc gtgccaggga tattaccttt
    19141 agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag tcacacaagc
    19201 attattatgg ctgactggaa tattaattgt gctagcagga atcggtattt tataaaaaga
    19261 agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc gtttgatcca
    19321 tgggcaagta atggtaacgt tatctcaaaa aagtggagtg aattccattt ttgttgtaga
    19381 cgaagttgta gcaaaagata aatttatgag agatctttac aaaaatgcag gaagccgtac
    19441 aggacaaaaa acaattgtca ttacaccaga gaaagctaaa ttctattggg atgaatatca
    19501 atttaaagaa tacaattgta ttttgatcgc aaaaacagtc caaacgattt acgacttagt
    19561 aaaacacgga atccccatgg aagaattgaa tattggaggg attgctcaaa aaaatcctga
    19621 tacagatctt ttagtgacga aatctgttta tttaaataaa tcagatgctg aaaaattgaa
    19681 agaaatgaat gaacaatatg gcgtaaagaa tatttatttt caagccactc catctgcacc
    19741 taaaacagga ttaaaagatg tattgaacaa gtttgatttg tcataaacaa atgcttagaa
    19801 aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt gggaatttcg
    19861 ttatcgctat atttaccgta agcgccgtac aaaaaagagt aaacagcgtt ttctttcagc
    19921 attagtatca gatatttaca gtatgagaac agatgtgaca gtcattgctt acgatacgct
    19981 agcctaccga tcaaaaaata tttatgttgg agatatcgaa aaagcagaga aagttatctg
    20041 tacttattac gatacacccg ttcatgcatt aggaagttat ttcatgtttg attggaaaga
    20101 ccaaaggaaa aaaacaatct actctatatt attgtccttc attttgttgt tcagtttagg
    20161 ttggtgggga atgatgatat ataacaaaaa tcctcatcac gtattcgatc tgctatccgt
    20221 ataaacaggt atcactgtac tagcatttgg gagctacttt ttcttattgg gaaaagcagc
    20281 tcgcggatgg cctagcagac aaacttttat tcgaaatact tcatccatat taacgatgct
    20341 agaaatgatc cgtacgattg atgatccgag tgtagcgtat gcgtttgttg atgaaggctg
    20401 ttatggagaa aagggcttag acagcgtgag atcgggtatg aaaaaagagg ctattctttt
    20461 ttatctagac tctgttgggg cagatacacc gctacagttt tcgggaaatt atttttcaaa
    20521 taaagaacag tggctgaaac aagttgataa gttgaaagaa aaaaacgtca attatatttt
    20581 tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc aaaacagatt tacgagggaa
    20641 aacatttaat tggcaaaatg ccaatcagat tattgctctt tttcgataag taaaaagtgc
    20701 aaaacaagga gtgagaagac taatgttgga aatcatgatc gttacacatg gtaagttgag
    20761 cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca tcgagccata ttgaaacaat
    20821 gtgcttagag tctggggagg acatccaaac attcggagag aaggtcaaag aaaaattaac
    20881 tatgatcaac acagcagaac ccatgctagt atttgttgat ctaattagcg caagtccgta
    20941 caatcaagcg ctcttggcag tcaatgaact gactgaagaa aagaaagcag ccacttatgt
    21001 catagggggc gtgaatctgc caatgttgtt agaagcaatc aatcatcaga tgcttcacac
    21061 accgatagaa gaagcggtgg aacaaattgt caaacaagga aaagataatt taactgtttg
    21121 gcatgcttca gcggaagaaa aaaaattaga tgaggacgat ttttagaagg aaggctgaaa
    21181 agatgaagtg gggatttaga tggtatggtg aaaaagggga ttcgatccca ttaaaatatg
    21241 ttcgccaaat accgggaata aatgggataa ttggaacttt gctgactaaa atgcctggag
    21301 atgtttggga gatttcagaa attgaggagt tgaaagcatc tgttgagagc caaggactgg
    21361 aactgcttgg aattgaaagt gtagcgatcc acgatgcaat caaggcaggg acagatgaac
    21421 gagattacta tattgataac tatatacgaa caatcaaaaa cttgtctcga tgtggggttc
    21481 gattgatttg ttatagcttc aaaccaattt ttggatgggc gaaaactgat ttgtttaatg
    21541 aaaacaacga tggaagctat tctcttttat acgatcagaa aatcgtcgat cagatggagc
    21601 caggagaaat gtatcaattg atacacagcc agtcaaaggg attcaaattg ccgggttggg
    21661 aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga atgaccgaag
    21721 aagaattatt tgaaaatctg acttattttt tagaaaaagt gattccagtg tgcgaaaaag
    21781 cagatgtaaa aatggctatc catccagatg atccgccatg ggaacttttt ggttttccta
    21841 gagtgacgaa aaatcttggc gacttgaaga aaattctaaa tgcagtagat tctccatata
    21901 atggactaac gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat ttagttgaaa
    21961 tcatccatga ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg gagtttttag
    22021 gagaaagaaa gttcaaagaa acggcacatc cctctacaga aggctccctt gatatgtatc
    22081 atattatgaa agcgttggtg caagaagggt tcgacggtcc tatacgccct gatcatggta
    22141 gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct gtatgatcgt gcaatgggac
    22201 tcacttacat gcaaggtcta tatgaggcga taagtaagga aaaaccactg ggagagcatt
    22261 atgaataaaa tcaagctctt catctttgat atggatggcc ttttattaga aactggacgt
    22321 ttagcttatc gtgcttatgt aaaatcagca caaaagtatg attatgaaat gcgtaaggaa
    22381 gtctattatt tactaactgg ccaaacagaa atggcgataa gagaacaaat gggcattttg
    22441 tacggagaag atgttccata tatcaaatgg cgagaagcaa tcaatcaata taaagaaaaa
    22501 atcgtgaaag aagataagag agtatatacg aaaaaaggag cagaagaaat tttatctttt
    22561 gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta atacacgggt aaaagtagaa
    22621 atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc aggggatgat
    22681 gtaaaaaaag gaaagccgga accagagatc tttttgaaag cttgttcaaa aatgaatatt
    22741 cctccaagtg aagcacttgt atttgaagac tcgattgcag ggatcgaagc agcaaggcga
    22801 gcgggcattt tgtcttttct catcaaagat catttgatag ggttgcccga tcataatggg
    22861 aagcataagt tgaaagtaga tgtaagttta tatgaatcca ataaagcaga ctatgaattt
    22921 tatgacctgc atcaagcaaa acgattttta gaaagtaaag ggttgtatct ctaataaaga
    22981 ggactatttt ctagtcctct ttatttattg tcctgcacga ctgagggaac agatgaaaaa
    23041 gaagtaattt tttattttga agtatctacc ttatttgtag tgaacaattt agaacaaaca
    23101 caaaacgcat ctactgttat ctaccagtag atgcgtttaa agagaagaac gagaatcgtc
    23161 catatgaggg tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc
    23221 gtaacacctg tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa
    23281 tctgtatcca aggttactcg atattgtata tctttgttat agaatgtcat agccattctc
    23341 ctttctttaa atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa
    23401 gtataccacc aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat
    23461 aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc
    23521 gattcgaaga caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt
    23581 agaaaaagca tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat
    23641 ctacatagat aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt
    23701 gatacgtatc aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga
    23761 caaaggtttt ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc
    23821 tatccaaggt gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga
    23881 agatggtcaa cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt
    23941 taaccgtctt ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga
    24001 atgggctgaa tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg
    24061 aaaatacgaa taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt
    24121 cttgaaaatg aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat
    24181 tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc
    24241 taaattttta agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt
    24301 tgaaggaata cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc
    24361 aagtgatgaa agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt
    24421 aataattttt ttattttttg tgctttcgcc gttattgtag agtgatttag cattcttatt
    24481 tttaaaaata actgtgttat atattaaaaa aggcataata gttataagct tttttaaaga
    24541 atatttagaa attattaatt ttcgtctgaa aacaaaaaaa gaaagcgtta ttattttagt
    24601 ctattttcag ggggggaacc ttatgataaa aaaactattt ttactatcaa tattaacgct
    24661 agcaatggca ggttgttctg actctggctt agcaactgaa ctgatcacaa aatcaactga
    24721 agccacgaca gaggagttaa aatgtggaaa cgatcacagt gactacagag gcttaaatcg
    24781 acgatgcatg ggaatattag gcagaagcaa ttgtaagcat cgatgtagta atcgaagaag
    24841 gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa agaagcaatc tgtgctgtgc
    24901 atcgtataca gacgtcgaag atgcggtact tttaaaccac gtcaggagat gttcatttat
    24961 gtctagggaa gttggcgatg aaccaaaact agagtacgct tttagttttg cacgtgagga
    25021 tgaagataac cctaaaactt acgacatttt agcttgatct aagatggtta aagcttttta
    25081 atgaagtaag aggagataga aggcttttct agttatatta ggtaagataa attccttttt
    25141 gctccaaatt tgctaaacat tctgctagat aaacttcatc gtgttccgga taaatcggca
    25201 cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca tgatttgcca cgatacatag
    25261 gatttttcca ctctggtgct gggctgtaac cacgtttttc catttcatcc atgacaagct
    25321 gatgatattg gaataatcta taaggagaat agtgaaaaac ataatccaca gtagcatgtt
    25381 tctttcccca gccatttcct cttaatgcac agcattcccg atgttgtcct aaaagctgtt
    25441 gacgaggaag ctttgaaatc aatttttcat gccataatcg catagaaatg tctcctttat
    25501 atatagattg ttaaccttag acgttaaatg aaaaaaccag caccaaggct ggaaaaaatc
    25561 acttcagttt gcttgctgtt tctgagcgac caattaggta atcaacggaa atatcgtaaa
    25621 agtcagcgat atagattaat tcaatgatgc ttggaatttc ttctccttct tcgtacttac
    25681 gatatttgaa taaaggaata cttaacgcgc tagaaacagc tacttgagat aaagatcggc
    25741 cagttcttaa gaaattcaga cgacaacgta aagtatccag taaaatagct tcttcgatac
    25801 tcatttaatc acctttcatt atagtagaat agttataatg actatagaaa gctacacatg
    25861 caatcaatag tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata
    25921 aagacaaaac cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt
    25981 aattaaaaac tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc
    26041 gttctttgtt ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc
    26101 aaaacaaaac tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa
    26161 agataacatg ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga
    26221 tcaatgaaaa atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga
    26281 atgtgtctta tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat
    26341 aaaaagagga cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa
    26401 acatatttaa caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc
    26461 a
//
LOCUS       DACDSR010000031        25501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDSR010000031 DACDSR010000000
VERSION     DACDSR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.031"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWK15_001347"
     CDS             133..3660
                     /locus_tag="IWK15_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9546945.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWK15_001348"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWK15_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9546946.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWK15_001349"
     CDS             7448..7789
                     /locus_tag="IWK15_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9546947.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWK15_001350"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWK15_001350"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9546948.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWK15_001351"
     CDS             9146..11566
                     /locus_tag="IWK15_001351"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9546949.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWK15_001352"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWK15_001352"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9546950.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWK15_001353"
     CDS             13000..13737
                     /locus_tag="IWK15_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9546951.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWK15_001354"
     CDS             13751..14566
                     /locus_tag="IWK15_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9546952.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWK15_001355"
     CDS             14579..15226
                     /locus_tag="IWK15_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9546953.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWK15_001356"
     CDS             15242..15898
                     /locus_tag="IWK15_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9546954.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWK15_001357"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWK15_001357"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9546955.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWK15_001358"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWK15_001358"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9546956.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWK15_001359"
     CDS             18240..18758
                     /locus_tag="IWK15_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9546957.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWK15_001360"
     CDS             18865..19365
                     /locus_tag="IWK15_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9546958.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWK15_001361"
     CDS             complement(19645..20565)
                     /locus_tag="IWK15_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9546959.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWK15_001362"
     CDS             20730..21743
                     /locus_tag="IWK15_001362"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9546960.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWK15_001363"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWK15_001363"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9546961.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IWK15_001364"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IWK15_001364"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9546962.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IWK15_001365"
     CDS             complement(23383..24144)
                     /locus_tag="IWK15_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9546963.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IWK15_001366"
     CDS             24333..25427
                     /locus_tag="IWK15_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9546964.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 g
//
LOCUS       DACDSR010000032        25462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDSR010000032 DACDSR010000000
VERSION     DACDSR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.032"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..459)
                     /locus_tag="IWK15_001367"
     CDS             complement(<1..459)
                     /locus_tag="IWK15_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9546965.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKI"
     gene            complement(461..1129)
                     /locus_tag="IWK15_001368"
     CDS             complement(461..1129)
                     /locus_tag="IWK15_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9546966.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1259..1843)
                     /locus_tag="IWK15_001369"
     CDS             complement(1259..1843)
                     /locus_tag="IWK15_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9546967.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2174..2407)
                     /locus_tag="IWK15_001370"
     CDS             complement(2174..2407)
                     /locus_tag="IWK15_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9546968.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2422..3372)
                     /locus_tag="IWK15_001371"
     CDS             complement(2422..3372)
                     /locus_tag="IWK15_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9546969.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3369..4211)
                     /locus_tag="IWK15_001372"
     CDS             complement(3369..4211)
                     /locus_tag="IWK15_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9546970.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4213..4932)
                     /locus_tag="IWK15_001373"
     CDS             complement(4213..4932)
                     /locus_tag="IWK15_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9546971.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5624..5782
                     /locus_tag="IWK15_001374"
     CDS             5624..5782
                     /locus_tag="IWK15_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9546972.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            5854..7080
                     /locus_tag="IWK15_001375"
     CDS             5854..7080
                     /locus_tag="IWK15_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9546973.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7168..7560)
                     /gene="rpsI"
                     /locus_tag="IWK15_001376"
     CDS             complement(7168..7560)
                     /gene="rpsI"
                     /locus_tag="IWK15_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9546974.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7576..8019)
                     /gene="rplM"
                     /locus_tag="IWK15_001377"
     CDS             complement(7576..8019)
                     /gene="rplM"
                     /locus_tag="IWK15_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9546975.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8538..9005)
                     /locus_tag="IWK15_001378"
     CDS             complement(8538..9005)
                     /locus_tag="IWK15_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9546976.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9125..10663)
                     /locus_tag="IWK15_001379"
     CDS             complement(9125..10663)
                     /locus_tag="IWK15_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9546977.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10743..11429
                     /locus_tag="IWK15_001380"
     CDS             10743..11429
                     /locus_tag="IWK15_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9546978.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11630..12157)
                     /gene="msrA"
                     /locus_tag="IWK15_001381"
     CDS             complement(11630..12157)
                     /gene="msrA"
                     /locus_tag="IWK15_001381"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9546979.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12241..12918)
                     /gene="pgmB"
                     /locus_tag="IWK15_001382"
     CDS             complement(12241..12918)
                     /gene="pgmB"
                     /locus_tag="IWK15_001382"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9546980.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12915..14045)
                     /locus_tag="IWK15_001383"
     CDS             complement(12915..14045)
                     /locus_tag="IWK15_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9546981.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14045..16228)
                     /locus_tag="IWK15_001384"
     CDS             complement(14045..16228)
                     /locus_tag="IWK15_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9546982.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16225..17250)
                     /locus_tag="IWK15_001385"
     CDS             complement(16225..17250)
                     /locus_tag="IWK15_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9546983.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17295..18122)
                     /locus_tag="IWK15_001386"
     CDS             complement(17295..18122)
                     /locus_tag="IWK15_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9546984.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18119..19186)
                     /locus_tag="IWK15_001387"
     CDS             complement(18119..19186)
                     /locus_tag="IWK15_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9546985.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19212..20048)
                     /locus_tag="IWK15_001388"
     CDS             complement(19212..20048)
                     /locus_tag="IWK15_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9546986.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20045..20914)
                     /locus_tag="IWK15_001389"
     CDS             complement(20045..20914)
                     /locus_tag="IWK15_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9546987.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20984..22267)
                     /locus_tag="IWK15_001390"
     CDS             complement(20984..22267)
                     /locus_tag="IWK15_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9546988.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22281..23966)
                     /locus_tag="IWK15_001391"
     CDS             complement(22281..23966)
                     /locus_tag="IWK15_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9546989.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24241..25266
                     /locus_tag="IWK15_001392"
     CDS             24241..25266
                     /locus_tag="IWK15_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9546990.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7621 a         5168 c         4309 g         8364 t
ORIGIN      
        1 aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag
       61 atctaataat tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa
      121 gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg
      181 atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga
      241 gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc
      301 attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag
      361 actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata
      421 tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga
      481 aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt
      541 aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt
      601 cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag
      661 gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt
      721 cagaacgata agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg
      781 gtaaatgtat tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca
      841 accatgagat cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa
      901 atatagtaaa aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg
      961 aataaatcaa gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg
     1021 tagcaataat gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg
     1081 gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc
     1141 caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac
     1201 agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt
     1261 aactcagtaa ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca
     1321 tagtcttttc ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt
     1381 aaatgtagaa gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag
     1441 tctttaactt atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa
     1501 caatatcttg atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat
     1561 tctcaaaaat ataattaata aatggatcta attcattgaa gttctctctc caacttttct
     1621 ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa
     1681 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
     1741 gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt
     1801 gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta
     1861 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actttattat
     1921 cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc
     1981 taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag
     2041 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
     2101 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
     2161 aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat
     2221 cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac
     2281 ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt
     2341 ttctttttga taattcctga attgctagta aataaattag tatagtagtc gttttaattt
     2401 gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca
     2461 tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa
     2521 aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc
     2581 cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg
     2641 ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg
     2701 cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt
     2761 atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc
     2821 aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa
     2881 ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg
     2941 atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc
     3001 tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc
     3061 atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt
     3121 ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact
     3181 gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt
     3241 aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga
     3301 acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca
     3361 ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga
     3421 aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta
     3481 aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata
     3541 aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac
     3601 attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat
     3661 agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat
     3721 ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa
     3781 accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat
     3841 aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct
     3901 aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt
     3961 ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta
     4021 atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc
     4081 ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata
     4141 actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa
     4201 ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa
     4261 attttcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
     4321 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
     4381 ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc
     4441 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
     4501 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
     4561 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
     4621 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
     4681 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
     4741 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
     4801 aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag caccttcttg
     4861 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
     4921 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
     4981 aaaaattata ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag
     5041 attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa
     5101 aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa
     5161 attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa
     5221 attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac
     5281 ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa
     5341 actatttttg atcattgaca attaaataca atttttgaat aacgatccct gctattcgag
     5401 ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt
     5461 tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc
     5521 cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat
     5581 aactactatt ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac
     5641 ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta tatataattt
     5701 cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat
     5761 gaatacattt aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat
     5821 atactgcctt acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa
     5881 tataaaacta atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa
     5941 aagtatcaaa aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag
     6001 aagaaaatag aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa
     6061 ataacgttta aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca
     6121 attagatcaa acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg
     6181 ttgcatattt tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag
     6241 ataatatcag atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa
     6301 ggatatgttc gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga
     6361 actactaaaa ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga
     6421 atacaagaag gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta
     6481 aatgatgatt ttaataatca tttacttact tttcatgatt acacactttt tatgattacc
     6541 ctgtatcttg gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt
     6601 gaaaaacaaa cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca
     6661 aaaggtcgaa aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa
     6721 tggaaatcgg tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag
     6781 tatctcttta cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac
     6841 ctaaactatc gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca
     6901 cacaaactcc gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa
     6961 atttcaaatg ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca
     7021 gatatagttg ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga
     7081 tatgtgttct taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt
     7141 gccagaaacg ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc
     7201 tttcttaaga cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc
     7261 acgtttaaga gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg
     7321 acggatagct cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata
     7381 tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg
     7441 tggaatgtat tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag
     7501 acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa
     7561 tgaaattccc tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc
     7621 atgtggatgt tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt
     7681 gtttttagga agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg
     7741 caattcgcca gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat
     7801 tttgtcagtc gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa
     7861 atcgccagta tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc
     7921 aacaactgtt gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg
     7981 ttctacttcg cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag
     8041 ttctgatgtt tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat
     8101 ttaacatatt acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt
     8161 atatgaaact ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga
     8221 aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg
     8281 ttctcctgcc aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt
     8341 ttaaaattgt ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg
     8401 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
     8461 tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc
     8521 aagttcaaca acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc
     8581 atccagatac gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg
     8641 tcgccttctt ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag
     8701 cctgcaacaa gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca
     8761 gggatttttg tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg
     8821 atcaagcgtt cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc
     8881 attgggtgca atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca
     8941 acagacattt ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat
     9001 ttcatctttc ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga
     9061 aaagtacaac aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag
     9121 ccaatcattt ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct
     9181 gtgctaaatg cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat
     9241 cagtttcttg tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac
     9301 tttggatctt tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg
     9361 tgacagtagg gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg
     9421 gagattgagc aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc
     9481 cttttaaaat ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt
     9541 tttctgtatg ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg
     9601 gtgctcgctc accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa
     9661 aagcgatagc cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa
     9721 cgacttcctg taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg
     9781 ctcctcccac tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat
     9841 ggaactgagg ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg
     9901 cgatcgtatc accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca
     9961 aaacaaatga tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg
    10021 ttacttgata ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt
    10081 caagcacttc ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg
    10141 ttccgtagtc gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga
    10201 caaaataagc agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt
    10261 tcaatggtgt cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca
    10321 cggtatattt cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc
    10381 gagataacgg tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg
    10441 agacaaattg aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc
    10501 agacaccttg aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca
    10561 aagggtagcg aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt
    10621 tggtttgtgt ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt
    10681 tatgtatgta caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg
    10741 gcgtgttact ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc
    10801 agcggattcc gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc
    10861 gttcgctttc ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct
    10921 gccgctattg caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg
    10981 gtctttttgc ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga
    11041 tgctttcggc tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca
    11101 cgctattctg ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat
    11161 ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct
    11221 atctcggagc tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag
    11281 agttgcttca gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact
    11341 ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt
    11401 tgctgattgt ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac
    11461 ctctgatata tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca
    11521 caacctctcc actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg
    11581 ttttacaacc tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga
    11641 cgccccaatg tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat
    11701 ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc
    11761 ttggttcgat ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt
    11821 ttgaacgctc tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag
    11881 aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga
    11941 tttccaccaa ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg
    12001 tatgccctgt tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag
    12061 tataaccgga tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc
    12121 aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat
    12181 cataccttga tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta
    12241 ttatatacgt gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt
    12301 taaatcagga atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac
    12361 atgcatgtta ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca
    12421 tgcatttggc ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt
    12481 tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt
    12541 ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt
    12601 tttcaagtac tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg
    12661 aatcgcttga agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga
    12721 gaatttctgt tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat
    12781 tcctttcagt tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac
    12841 cagccacgct tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag
    12901 tgctgctttg attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg
    12961 atgtttttag ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc
    13021 acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt
    13081 cctttaccaa cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca
    13141 atcccaactg gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt
    13201 acgaggtctg cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg
    13261 ccagagacta ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca
    13321 ctactgcccg atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct
    13381 tggtcagcca ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa
    13441 gcgccttctt ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta
    13501 atcagctcaa taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc
    13561 agtgcttcta ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg
    13621 ccgatgccgc catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct
    13681 tgttgcaaca attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt
    13741 tgttggttta gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt
    13801 tgaccgtctg aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct
    13861 gtttgccgct tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg
    13921 cctataggta gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg
    13981 gcttgtgctg cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata
    14041 cgcattattt tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat
    14101 tgacaaaaat aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca
    14161 aaacaaatag caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg
    14221 aaggaagtct tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa
    14281 atataactgc tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat
    14341 gcggatctgg tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca
    14401 tttcttggat acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga
    14461 ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag
    14521 gtaataagta taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt
    14581 cgctacgggc ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca
    14641 aatcaataac tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag
    14701 gcactggcaa atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc
    14761 cagcttgata tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat
    14821 tattgtcgat atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat
    14881 catcgacttt tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc
    14941 cctttgtttc taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag
    15001 caaggtctgc actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg
    15061 ttttgatatt caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc
    15121 atgggaaaag tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc
    15181 gatcataacg gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag
    15241 gcaggataaa aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc
    15301 ccttcgcccc tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag
    15361 cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct
    15421 gatgccaaaa atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc
    15481 ttttctgggc ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga
    15541 gcacgatgat cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag
    15601 cggtgatccg gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc
    15661 caatatcaat tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata
    15721 ctcgcagttc tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac
    15781 ccgttttcac atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg
    15841 cctgatggag attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt
    15901 gagcagtttc aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct
    15961 cgtatgcaag aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc
    16021 atctgatgac atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat
    16081 atacaccagc tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc
    16141 tgataccaaa aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta
    16201 aatcaaactg tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg
    16261 atataaattt tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg
    16321 agtctggtca ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt
    16381 aactggcgtg gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc
    16441 tggatacaga ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct
    16501 gatattcaaa gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt
    16561 gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga
    16621 attttttttc gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa
    16681 tccagtaata tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc
    16741 tccttgaagt tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc
    16801 attcgcttct acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc
    16861 ttcgtcagaa aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt
    16921 ctctgccatc tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga
    16981 agcattagct tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac
    17041 actcacgata tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga
    17101 aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc
    17161 caattctaag acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg
    17221 tccagcgcct atgattgcta ctttcttcat ttatttctct ctcctttgct tatttactta
    17281 ttgttattct ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag
    17341 tttttcaagt attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga
    17401 cattcatata ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta
    17461 aaatcaattg cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc
    17521 acatactctt tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga
    17581 taaaaatcag cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca
    17641 tccgctaaca aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc
    17701 gtacctgttt cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag
    17761 tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa
    17821 gctgctggga taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca
    17881 atttgacgta caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag
    17941 ccgttacaag ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg
    18001 atattttcta ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata
    18061 aatctaaatt tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc
    18121 attgttctcc tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta
    18181 cataacgcat gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt
    18241 cacaatccaa gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt
    18301 tccatcttgg gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat
    18361 tattgaaatg tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa
    18421 cataagaaat gatcccgcca tagccgatcc ctattaaaga agcttgtaat gcggcagcac
    18481 tgccactcgt ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa
    18541 aaccgacatc tgctttcata ggatctagcg tgtaatcggc accgtgtttt tctgctaatt
    18601 ctctacgctg cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta
    18661 attgaatcgc aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc
    18721 gaacatgtgc ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat
    18781 ttttccattg atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa
    18841 tcagcgtttc acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca
    18901 ccaaactgcc cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa
    18961 ataccacacc gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa
    19021 attcttcatc gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg
    19081 gagtagcgaa aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat
    19141 ctttgaatcc ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct
    19201 ttcttttctt tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca
    19261 gataacgcat acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt
    19321 ccccaaatgt agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt
    19381 tcggaggtcg acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa
    19441 taaacgaaga cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc
    19501 tggatccggc taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca
    19561 aaataacttt ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc
    19621 agtgctgtat tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg
    19681 atcaaaatac cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct
    19741 ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg
    19801 gaggctatca aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca
    19861 gcattgaaaa tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc
    19921 gttgcaattg cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac
    19981 aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta
    20041 tttttcattt caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga
    20101 acaatagaat cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat
    20161 acaaataaac gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat
    20221 agaacatcca gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca
    20281 gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa
    20341 gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc
    20401 tccagatagc aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg
    20461 gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat
    20521 tattgaacat atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata
    20581 aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg
    20641 ctaatcctaa agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta
    20701 aagaacgata aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa
    20761 cggcgtctga gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca
    20821 ttggaatcag cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta
    20881 attttaagtc agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca
    20941 atagctatct acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt
    21001 gtgcgtcagc taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt
    21061 ttccgatcac acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta
    21121 gtccaaagac ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag
    21181 acttgatgac ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt
    21241 gagcaccacc aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag
    21301 ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc
    21361 cagatactgt gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt
    21421 ctgagttccc cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg
    21481 ctgttccatt catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt
    21541 attgggataa gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta
    21601 cctcgccttc atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt
    21661 gctcactcat tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat
    21721 ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag
    21781 cttctgataa tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag
    21841 caatatagct ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat
    21901 cttctccagc ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat
    21961 ccatcacttt agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag
    22021 ctaacgtaac gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga
    22081 tgtctggatt ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct
    22141 gttcgacaga agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac
    22201 tttcaccaca agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt
    22261 ttttcatttt tttccacctc ttattttatt attttcagcc acatatattc atacggctgg
    22321 aatatcaact ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa
    22381 tctccttttg gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact
    22441 ttctcttcct tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa
    22501 atcaaactat tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat
    22561 tcaccatata ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac
    22621 aaaaattttt gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca
    22681 tttcgacttc ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa
    22741 aatgaatgag cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt
    22801 aacgcatcta gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc
    22861 ttgtagttta ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt
    22921 tccggcagaa tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta
    22981 aaaaaagttg tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt
    23041 gtattacccg aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc
    23101 tgtgcttcat tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc
    23161 tcagtgatca ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg
    23221 attttatgcg tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc
    23281 acagcgtcca agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc
    23341 attttcaata aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc
    23401 gtccatacgt gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc
    23461 cttggtctag tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg
    23521 tacgtatcat tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg
    23581 cagacaaagt cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg
    23641 cccaaacggt cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat
    23701 gggaaaaatg gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat
    23761 ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc
    23821 aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa
    23881 acactgactt tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa
    23941 atcgcttgta aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa
    24001 cgatttgatc atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga
    24061 tatacacaaa aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa
    24121 atatggtctc tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag
    24181 aatgtctatt gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa
    24241 ttgaaaccta ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc
    24301 agagtattaa ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca
    24361 atcaaagaat tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt
    24421 gacgcgattc tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt
    24481 tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta
    24541 caaacggcaa aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa
    24601 atgatccgag gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg
    24661 gatcaatata atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc
    24721 tactcagtgg acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag
    24781 aatggacacc gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat
    24841 cgtctcaatg gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg
    24901 attatagacg gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag
    24961 ctacgagaaa gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac
    25021 aaagtggcag aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt
    25081 gatcccttat tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat
    25141 cagttaggaa tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa
    25201 gaaaaaagaa tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa
    25261 atgtagaaca gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt
    25321 ttttcaatca ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt
    25381 ccatctgttt tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca
    25441 gggaagaaaa aactattttt aa
//
LOCUS       DACDSR010000033        23561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDSR010000033 DACDSR010000000
VERSION     DACDSR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.033"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..256
                     /gene="yaaA"
                     /locus_tag="IWK15_001393"
     CDS             14..256
                     /gene="yaaA"
                     /locus_tag="IWK15_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9546991.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            243..1367
                     /gene="recF"
                     /locus_tag="IWK15_001394"
     CDS             243..1367
                     /gene="recF"
                     /locus_tag="IWK15_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9546992.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1364..3310
                     /gene="gyrB"
                     /locus_tag="IWK15_001395"
     CDS             1364..3310
                     /gene="gyrB"
                     /locus_tag="IWK15_001395"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9546993.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3330..5801
                     /gene="gyrA"
                     /locus_tag="IWK15_001396"
     CDS             3330..5801
                     /gene="gyrA"
                     /locus_tag="IWK15_001396"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9546994.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6037..6336
                     /locus_tag="IWK15_001397"
     CDS             6037..6336
                     /locus_tag="IWK15_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9546995.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6386..6898
                     /gene="ssb"
                     /locus_tag="IWK15_001398"
     CDS             6386..6898
                     /gene="ssb"
                     /locus_tag="IWK15_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9546996.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6924..7160
                     /gene="rpsR"
                     /locus_tag="IWK15_001399"
     CDS             6924..7160
                     /gene="rpsR"
                     /locus_tag="IWK15_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9546997.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7303..9276
                     /locus_tag="IWK15_001400"
     CDS             7303..9276
                     /locus_tag="IWK15_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9546998.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9282..9734
                     /gene="rplI"
                     /locus_tag="IWK15_001401"
     CDS             9282..9734
                     /gene="rplI"
                     /locus_tag="IWK15_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9546999.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10008..11375
                     /gene="dnaB"
                     /locus_tag="IWK15_001402"
     CDS             10008..11375
                     /gene="dnaB"
                     /locus_tag="IWK15_001402"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9547000.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12155..13447
                     /locus_tag="IWK15_001403"
     CDS             12155..13447
                     /locus_tag="IWK15_001403"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9547001.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14183..15046
                     /locus_tag="IWK15_001404"
     CDS             14183..15046
                     /locus_tag="IWK15_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9547002.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15175..16797)
                     /locus_tag="IWK15_001405"
     CDS             complement(15175..16797)
                     /locus_tag="IWK15_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9547003.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17046..17567
                     /locus_tag="IWK15_001406"
     CDS             17046..17567
                     /locus_tag="IWK15_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547004.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17753..19483)
                     /gene="spxB"
                     /locus_tag="IWK15_001407"
     CDS             complement(17753..19483)
                     /gene="spxB"
                     /locus_tag="IWK15_001407"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9547005.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19648..20466
                     /locus_tag="IWK15_001408"
     CDS             19648..20466
                     /locus_tag="IWK15_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9547006.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20569..20766)
                     /locus_tag="IWK15_001409"
     CDS             complement(20569..20766)
                     /locus_tag="IWK15_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547007.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            20935..21513
                     /locus_tag="IWK15_001410"
     CDS             20935..21513
                     /locus_tag="IWK15_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9547008.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(22558..23061)
                     /locus_tag="IWK15_001411"
     CDS             complement(22558..23061)
                     /locus_tag="IWK15_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9547009.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT         7630 a         4201 c         4915 g         6815 t
ORIGIN      
        1 tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca
       61 aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga
      121 aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg
      181 catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga
      241 taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg
      301 aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt
      361 tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat
      421 tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca
      481 ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg
      541 aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag
      601 atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag
      661 gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa
      721 gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata
      781 ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg
      841 tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc
      901 agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa
      961 tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa
     1021 caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc
     1081 agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa
     1141 tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga
     1201 gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa
     1261 cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga
     1321 ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag
     1381 tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga
     1441 agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca
     1501 ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa
     1561 aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat
     1621 acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt
     1681 acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt
     1741 aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg
     1801 aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga
     1861 acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga
     1921 gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa
     1981 ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata
     2041 ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat
     2101 ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat
     2161 gcaatataca gacggctacc attccaatat catgagtttt gccaacaata tccatacgta
     2221 tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta
     2281 tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg
     2341 agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac
     2401 aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca
     2461 tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt
     2521 actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg
     2581 tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga
     2641 aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg
     2701 taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc
     2761 aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac
     2821 agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac
     2881 tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta
     2941 catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt
     3001 aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa
     3061 acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact
     3121 tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat
     3181 ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg
     3241 ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt
     3301 agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg
     3361 atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg
     3421 tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat
     3481 acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg
     3541 tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg
     3601 tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg
     3661 gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa
     3721 tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg
     3781 atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg
     3841 gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg
     3901 tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg
     3961 aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc
     4021 gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg
     4081 agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag
     4141 caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag
     4201 atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg
     4261 ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg
     4321 gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc
     4381 ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag
     4441 aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg
     4501 atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga
     4561 tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc
     4621 gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga
     4681 ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg
     4741 aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg
     4801 aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga
     4861 caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg
     4921 gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt
     4981 gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag
     5041 gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag
     5101 gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag
     5161 gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt
     5221 ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg
     5281 tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat aacggctact
     5341 ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag
     5401 gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag
     5461 aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag
     5521 tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac
     5581 tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg
     5641 tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac
     5701 gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag
     5761 ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt
     5821 aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt
     5881 tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag
     5941 tgtataatca gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa
     6001 cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc
     6061 atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc
     6121 gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc
     6181 cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct
     6241 tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat
     6301 atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag
     6361 aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac
     6421 gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc
     6481 tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt
     6541 gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg
     6601 tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt
     6661 gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg
     6721 ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa
     6781 taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc
     6841 tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac
     6901 gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta
     6961 aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac
     7021 taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg
     7081 ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac
     7141 ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct
     7201 attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa
     7261 agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat
     7321 catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc
     7381 ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt
     7441 gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag
     7501 caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga
     7561 gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa
     7621 atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa
     7681 gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca
     7741 gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag
     7801 acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga tgccattgat
     7861 aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac
     7921 tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta
     7981 gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt
     8041 cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc
     8101 tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc
     8161 cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat
     8221 ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc
     8281 gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg
     8341 gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa
     8401 aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa
     8461 gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag
     8521 ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc
     8581 tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg
     8641 gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca
     8701 gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt
     8761 gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca
     8821 actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta
     8881 gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct
     8941 ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat
     9001 aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca
     9061 gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc
     9121 acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca
     9181 gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc
     9241 aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac
     9301 aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac
     9361 aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt
     9421 tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa
     9481 agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg
     9541 aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac
     9601 aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag
     9661 gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg
     9721 tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa
     9781 aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa
     9841 atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac
     9901 gagtataata aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct
     9961 ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt
    10021 ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt
    10081 ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc
    10141 gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga
    10201 ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg
    10261 gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact
    10321 atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa
    10381 tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac
    10441 gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag
    10501 tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag
    10561 gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac
    10621 taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc
    10681 aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag
    10741 aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta
    10801 caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc
    10861 gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat
    10921 gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt
    10981 tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac
    11041 tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg
    11101 gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa
    11161 ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag
    11221 gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg
    11281 agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata
    11341 agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa
    11401 tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa
    11461 tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat
    11521 aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa
    11581 ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt
    11641 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga
    11701 gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    11761 tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat
    11821 atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa
    11881 cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa
    11941 aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta
    12001 caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata
    12061 aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg
    12121 ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac
    12181 gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt
    12241 gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac
    12301 ttataaactg catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg
    12361 aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa
    12421 aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca
    12481 tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat
    12541 taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga
    12601 cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg
    12661 tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata
    12721 ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga
    12781 tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat
    12841 cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat
    12901 cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac
    12961 gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat
    13021 ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt
    13081 tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa
    13141 ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga
    13201 tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat
    13261 ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact
    13321 gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc
    13381 aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca
    13441 aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt
    13501 ttatctatac gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa
    13561 aaccgtgtca cagtcccttt ttccgaacaa acggtgttca aaagcttgca tctacggcac
    13621 taagcccaat cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg
    13681 atgcagagca gctttttcac aacctctcca ctaaacggtg ttctcctgcc aactcctcgg
    13741 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac
    13801 tcggagctaa acggcaggct cacaacctct tggtaaacgg tgttcaatca gctgaccctc
    13861 ggtattagct caaaaaacgg caaaatatga ggaactattt tttccgtttt tttctctaat
    13921 actcaggtca aaacgctgat ttcacaacct ctgattcacg gtgttccaga agtagcttct
    13981 acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    14041 tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact tgcgaaaata
    14101 aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa gggtaaaatg
    14161 taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt gtgatctgcc
    14221 ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc aattgaatga
    14281 tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca tgaatcagct
    14341 gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt tagaattttt
    14401 ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca gtggattaag
    14461 cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat atgaagaccc
    14521 tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt tagtattgga
    14581 agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt ggctggaaag
    14641 taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc tagaacgagg
    14701 cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa agcctattat
    14761 tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc
    14821 gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt
    14881 ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc ttttggaaga
    14941 gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg ccagtcacac
    15001 aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc agtaactcat
    15061 tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa agatagatga
    15121 gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag gccattatgc
    15181 ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat catatgtcgt
    15241 atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg ccaatgtttg
    15301 gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca tcaagccgtc
    15361 attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta atacaagtac
    15421 gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc caccagataa
    15481 aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta agaagcttct
    15541 taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt ctaaaatcgt
    15601 tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac ttgttgtcac
    15661 accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt tgaatagcgt
    15721 tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa
    15781 agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt ctacctgcaa
    15841 taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac gtctagaaga
    15901 aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag ttgcttgttt
    15961 tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt tgatttgttc
    16021 ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag attccaacca
    16081 aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg ccatatgtgt
    16141 acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat tttcaaaatt
    16201 gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg ttggttcgtc
    16261 tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt ttaccttttg
    16321 tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt tcagaccttg
    16381 cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa attctccttc
    16441 taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc
    16501 tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt caagtacagt
    16561 atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat ctggtcccat
    16621 agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga acgttgattt
    16681 acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt taatattcac
    16741 atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag taatcaattt
    16801 ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata taggggaaag
    16861 gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa
    16921 taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc aacagcataa
    16981 tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg aaagaaggat
    17041 tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga attttatttg
    17101 tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca attgtagtag
    17161 tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg aacagattaa
    17221 aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc gatattctag
    17281 ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct tttgtttttg
    17341 cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg gcaagaggag
    17401 taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt ttattaggaa
    17461 ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta gttggattct
    17521 attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta aaatcaaaca
    17581 aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc ggctttttta
    17641 tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt
    17701 gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt tgtcacaacc
    17761 tcttttcata taattcattc aatactggca aatcatggac ttcatatttt tctttgaatg
    17821 cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct actggcaatg
    17881 ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt gcagctgcat
    17941 caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc ccgagcgctt
    18001 ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt tgttccgtat
    18061 cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca ttaataattg
    18121 gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac ccgccatcac
    18181 cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt cctcctggca
    18241 caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta ccgttcatat
    18301 tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt
    18361 cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt tcttcctctc
    18421 gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga tttgctaggt
    18481 accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa gctgtgtttg
    18541 catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat
    18601 cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg aagtcgctac
    18661 cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta gtagcgacac
    18721 gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt gctagaacgg
    18781 aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga aatccgtttc
    18841 gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa ggcaaagcag
    18901 ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga gcggtggaga
    18961 aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg atagccacgc
    19021 ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga ctttgtggtg
    19081 tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca tttagttcct
    19141 ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct actagaggca
    19201 catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag
    19261 agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct gcgagtgcgc
    19321 cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga tatagcgcat
    19381 ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata ccccagcttt
    19441 caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt cactccttta
    19501 gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc gaaaatccca
    19561 actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta gagtagtaca
    19621 tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact tgaaccttgg
    19681 cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg tgccgggttg
    19741 gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg atttgcttct
    19801 ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac aaaagcagaa
    19861 gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg cggtgtgttt
    19921 ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca tgaagcagaa
    19981 ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac attgcataac
    20041 attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc ggataatccg
    20101 acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca aaacttccca
    20161 gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt
    20221 gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg agcgctacta
    20281 gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg agcaatgatt
    20341 tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag caaacgtcat
    20401 tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga tgtggcatta
    20461 ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta tatcgcgacc
    20521 ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt aataatagtt
    20581 ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg cgattgggat
    20641 tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa gactgatgac
    20701 gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca tatttttttg
    20761 acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata acagggattg
    20821 tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat gatcaaacaa
    20881 ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa tatcatgcct
    20941 aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac tattttagtt
    21001 ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt aggttaccga
    21061 ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa tcatcatcat
    21121 ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag tttctttgtt
    21181 tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat caatagcctg
    21241 gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta ttatatcttg
    21301 gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc gatgttgaat
    21361 aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt cttagggtat
    21421 ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc ttggatcatc
    21481 ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa atttaagaga
    21541 aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta ttcaaagagg
    21601 gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg tcacgatgct
    21661 tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag gatttttgac
    21721 attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct atctatttaa
    21781 gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt gtggaaaatt
    21841 taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt ggataacttc
    21901 gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt ttgataggag
    21961 atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa cggtgtggtt
    22021 tttggaataa aaaaataaca tatctcattt tacgcttttg ttattttata cttaaaaaat
    22081 aacgacacag aaaataccgt gtctcagatt tctctactac ttctaaaaac tactgttttt
    22141 ttcctttgaa atgccttcaa aaagtaggat caggcagaat atactggcag aatcaaggag
    22201 aagggagcct aaataaggag aaaaaccata gaaaatataa agtaattact tataaaacaa
    22261 gctttttata gaaaaataat atacctgagt agggtataaa ttaagacgga attcctttct
    22321 agattgagat attgactagg aagaatgtta agattttaag cctttaagct aaaaaaaaac
    22381 cctttttgtt aaataagggg aatatagcct gtcagtttca aactgcaagt cgtcctgctg
    22441 caataatata gtagattaga gatgaaaata gattttttta aaccaatgac tgaataaaaa
    22501 gaccgtcata gatacacatt ggaatgtgtc tattacggtc tttttttacg aacgatttta
    22561 ttgaagacgg acagttcggg tcaaaaataa aaaagctacg atcagaaata agaataaaat
    22621 gtatcccaaa ataatggcta gacggtctaa cttatttgtc gatagatgtt tgtttagaag
    22681 ttgataggcc cataaaccta tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc
    22741 cgctacgcca ataccgaata ggaattgaat caattctgcc aagaagctta caaggaaaat
    22801 aatccctaat tttttagaaa aagaaagtct cttgaaattg acattcaaaa gcaccccaaa
    22861 aggaaaaaag acaatcaagt tataagtggt ctcacgtaat accgtcgtat tttcaaaaga
    22921 aaaagtagaa aaagggacga gattgacatt gctgtaagaa taagtgagaa attgtggaag
    22981 attaaatgat agcttgaata aaatcaacca taatagaaag aataaataaa tccctaataa
    23041 aaattttgat acataccgca tagacatctc ctccatttaa aaagtatacc atcgaaaacc
    23101 gctgaaaaat cataaaaaag tatgaagttt aaggaccaac gtagttatcg tacgaaaatt
    23161 agaagctttt ttttgttggg aatccggttt ctttaacaaa gctaagatag cgagttgctt
    23221 ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat
    23281 ttctccaagt tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa tagttgttat
    23341 actaggtaat gctgacaaac gggattggca tttcatcttg gttcagcttt ttttccaatt
    23401 actaggaagg agggatagat tgttcaagac ttattttcgt ttcaataaaa tacaagataa
    23461 tccttttcgt atcatgaatc tgatgctact tatctttatt gtattatggt taggttacat
    23521 aaatcatgat tttttgattt caagtttttc atcattaggg a
//
LOCUS       DACDSR010000034        23077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDSR010000034 DACDSR010000000
VERSION     DACDSR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.034"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IWK15_001412"
     CDS             102..2543
                     /locus_tag="IWK15_001412"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9547010.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="IWK15_001413"
     CDS             complement(2873..3577)
                     /locus_tag="IWK15_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547011.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="IWK15_001414"
     CDS             complement(3764..4009)
                     /locus_tag="IWK15_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547012.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="IWK15_001415"
     CDS             complement(4026..4877)
                     /locus_tag="IWK15_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9547013.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="IWK15_001416"
     CDS             5043..6047
                     /locus_tag="IWK15_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9547014.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="IWK15_001417"
     CDS             complement(6288..6644)
                     /locus_tag="IWK15_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9547015.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="IWK15_001418"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="IWK15_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9547016.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IWK15_001419"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IWK15_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9547017.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11723)
                     /locus_tag="IWK15_001420"
     CDS             complement(11004..11723)
                     /locus_tag="IWK15_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9547018.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="IWK15_001421"
     CDS             12043..12564
                     /locus_tag="IWK15_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9547019.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="IWK15_001422"
     CDS             12623..13483
                     /locus_tag="IWK15_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9547020.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13590..15983
                     /locus_tag="IWK15_001423"
     CDS             13590..15983
                     /locus_tag="IWK15_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9547021.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="IWK15_001424"
     CDS             16165..18006
                     /locus_tag="IWK15_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9547022.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="IWK15_001425"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="IWK15_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9547023.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="IWK15_001426"
     CDS             19359..21458
                     /locus_tag="IWK15_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9547024.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="IWK15_001427"
     CDS             21654..22517
                     /locus_tag="IWK15_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9547025.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23077
                     /locus_tag="IWK15_001428"
     CDS             22622..>23077
                     /locus_tag="IWK15_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9547026.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7771 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaaa
//
LOCUS       DACDSR010000035        23065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDSR010000035 DACDSR010000000
VERSION     DACDSR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.035"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWK15_001429"
     CDS             complement(109..564)
                     /locus_tag="IWK15_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547027.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWK15_001430"
     CDS             complement(718..1926)
                     /locus_tag="IWK15_001430"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9547028.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWK15_001431"
     CDS             complement(2038..2370)
                     /locus_tag="IWK15_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9547029.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWK15_001432"
     CDS             2518..3393
                     /locus_tag="IWK15_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9547030.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWK15_001433"
     CDS             complement(3475..4056)
                     /locus_tag="IWK15_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9547031.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWK15_001434"
     CDS             complement(4062..5321)
                     /locus_tag="IWK15_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9547032.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWK15_001435"
     CDS             complement(5323..6369)
                     /locus_tag="IWK15_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547033.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWK15_001436"
     CDS             complement(6604..6879)
                     /locus_tag="IWK15_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9547034.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWK15_001437"
     CDS             complement(7122..8432)
                     /locus_tag="IWK15_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9547035.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWK15_001438"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWK15_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9547036.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWK15_001439"
     CDS             complement(9978..10658)
                     /locus_tag="IWK15_001439"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9547037.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWK15_001440"
     CDS             complement(10707..11342)
                     /locus_tag="IWK15_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9547038.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWK15_001441"
     CDS             complement(11408..12811)
                     /locus_tag="IWK15_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9547039.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWK15_001442"
     CDS             complement(12798..13829)
                     /locus_tag="IWK15_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547040.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWK15_001443"
     CDS             13860..14081
                     /locus_tag="IWK15_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9547041.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWK15_001444"
     CDS             complement(14246..14983)
                     /locus_tag="IWK15_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9547042.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWK15_001445"
     CDS             complement(15136..16476)
                     /locus_tag="IWK15_001445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9547043.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWK15_001446"
     CDS             complement(16713..18236)
                     /locus_tag="IWK15_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9547044.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWK15_001447"
     CDS             complement(18614..19225)
                     /locus_tag="IWK15_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9547045.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWK15_001448"
     CDS             complement(19555..20271)
                     /locus_tag="IWK15_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9547046.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWK15_001449"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWK15_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9547047.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWK15_001450"
     CDS             complement(20830..21627)
                     /locus_tag="IWK15_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9547048.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWK15_001451"
     CDS             complement(21597..21968)
                     /locus_tag="IWK15_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9547049.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWK15_001452"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWK15_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9547050.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DACDSR010000036        23004 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDSR010000036 DACDSR010000000
VERSION     DACDSR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.036"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            476..727
                     /locus_tag="IWK15_001453"
     CDS             476..727
                     /locus_tag="IWK15_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547051.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="IWK15_001454"
     CDS             761..1039
                     /locus_tag="IWK15_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547052.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1636..1893
                     /locus_tag="IWK15_001455"
     CDS             1636..1893
                     /locus_tag="IWK15_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547053.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2006..2617
                     /locus_tag="IWK15_001456"
     CDS             2006..2617
                     /locus_tag="IWK15_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547054.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2796..3353
                     /locus_tag="IWK15_001457"
     CDS             2796..3353
                     /locus_tag="IWK15_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547055.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4052..4573)
                     /locus_tag="IWK15_001458"
     CDS             complement(4052..4573)
                     /locus_tag="IWK15_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9547056.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4772..8464
                     /gene="nifJ"
                     /locus_tag="IWK15_001459"
     CDS             4772..8464
                     /gene="nifJ"
                     /locus_tag="IWK15_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9547057.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8653..9540
                     /locus_tag="IWK15_001460"
     CDS             8653..9540
                     /locus_tag="IWK15_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9547058.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9537..10706
                     /locus_tag="IWK15_001461"
     CDS             9537..10706
                     /locus_tag="IWK15_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547059.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10730..12085
                     /locus_tag="IWK15_001462"
     CDS             10730..12085
                     /locus_tag="IWK15_001462"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9547060.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="IWK15_001463"
     CDS             complement(12157..13962)
                     /gene="glmS"
                     /locus_tag="IWK15_001463"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9547061.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14371..14661
                     /locus_tag="IWK15_001464"
     CDS             14371..14661
                     /locus_tag="IWK15_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547062.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14674..15699
                     /gene="sppA"
                     /locus_tag="IWK15_001465"
     CDS             14674..15699
                     /gene="sppA"
                     /locus_tag="IWK15_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9547063.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15702..16361
                     /locus_tag="IWK15_001466"
     CDS             15702..16361
                     /locus_tag="IWK15_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9547064.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16518..17450
                     /locus_tag="IWK15_001467"
     CDS             16518..17450
                     /locus_tag="IWK15_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547065.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17604..18893
                     /locus_tag="IWK15_001468"
     CDS             17604..18893
                     /locus_tag="IWK15_001468"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9547066.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19140..20390
                     /gene="clpX"
                     /locus_tag="IWK15_001469"
     CDS             19140..20390
                     /gene="clpX"
                     /locus_tag="IWK15_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9547067.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20473..21060
                     /locus_tag="IWK15_001470"
     CDS             20473..21060
                     /locus_tag="IWK15_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9547068.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            21425..22753
                     /locus_tag="IWK15_001471"
     CDS             21425..22753
                     /locus_tag="IWK15_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547069.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            22764..22973
                     /locus_tag="IWK15_001472"
     CDS             22764..22973
                     /locus_tag="IWK15_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547070.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         7656 a         3915 c         4910 g         6523 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atgaccaatt acaaaataga ttgataaagc attttttgca tttttataat
     1441 agtttattga taataatgta tgagatacag tataatgatt taaaaggtaa cgtgttgtga
     1501 ccgaattaaa tgaattaatg cctatttttt atattcctta gtggacaaaa atggagacag
     1561 tgaagttagg agcattgtat cctttgttgc gtagttcaaa aaagtaagcg atttctataa
     1621 tggtcagggt aaacggtgag ggctgtccac gtaaatccta ctattgaaat cacaatgatc
     1681 ggacattcaa aaggtggtgc tgaagcagca aattcagtag ctataaatac gaatgcgatt
     1741 atatttaatc cagcgacagt aaatttatct gcttatgaat tagactcaaa cacatataca
     1801 gcaaacatgg tagcttatgt tgtcgaggga gatgcattaa atgttgtttt tggaggagta
     1861 tacacctatt ggagaattaa tcatgttaga tagacaatat gatgcacctt agtggagaat
     1921 gatatctcgc ataggtaact cagtaaaaaa tcaaggtatg gaagcaatta tctctgcatt
     1981 ggaagatcga tatagatagg cggatatgaa tagtaatagg gggaataaaa tggaatacaa
     2041 tggatcaatg agaattgtac aaattatatt ttggattaca tcgggaattg tgatcatagg
     2101 aggagttttt ttgatgttgc caagcttaat atttcctttc tttgcattaa tctcacctaa
     2161 aatacccgaa ccggaaatca cctatggaga atttccattt aggattgaat acgaactaga
     2221 tggacagatt aacataattg aagatactgt catagctgag tttaacgggt gtgagttcag
     2281 cgcaggttct atgaaaagag aacgaagatg gagaagtaga ttagctagtg gtagagaaga
     2341 tctacctttt ggggacgatc taggaatcta ttttagtcga ggaagtgcgc aatattatat
     2401 gggggaaaat gtacaatcaa tgtcattaaa accacatata gcccttagaa atctcgagga
     2461 aggttttaaa agagaggtag actttatatt aggagaaagt ggatacataa gaactgtact
     2521 taatttcgga gaagcgcaag aagttttgac acaatacgga attaccttaa tcaactggga
     2581 aatatctgaa ccgattgtta acaattttgg tgattagaaa acaattacga taaatagaaa
     2641 caagccaaat catcagcaag taaaagctga tgatttggcc tttcttatac gagtagtagg
     2701 aattcatagc gaatattatt acgaccagta gatattttga tattaaagga gtattacgaa
     2761 ctaatatgtg agaatgtaga ggtgatgtgc gaataatgag aaatttatca aatagaaaca
     2821 aaatattaat cattattgtt gttatagctg tgtttcatct gggaacgaat gcggttttaa
     2881 gtagaataat attgggtcct aagccaccaa gaccagaaat tacccgaggg gagtttgatt
     2941 ttagattaga atatgaagta gatggggaaa ggattgtgat agaagataca attgttgcat
     3001 tatttgatgg attttctgca gatgcaggta gcatggcatg gtatcgcacg tggcgtctac
     3061 atttagcaag tgatagacga tcacgaaata tcttattaga tgaattagag gatggtcgaa
     3121 gaatatatta tgtcccagaa agtgctaact attctatggg agatgtacag aaaaaaagag
     3181 agcctaatcc taattggtat ccatttaatg gcgtaacaat agaatatcca cgcaataaaa
     3241 cacctgaaat aggtgcaaaa tttataagtg gtttagaaga tctgtacgat aggtttggaa
     3301 tccgtcttat tagttgggaa cacgatccac caatagaaaa tcgctttgaa taatcgaaat
     3361 ctactaatcc atttcatcag ccagtgaatg ttgtaatgga acaatttggt ttagtgttca
     3421 aaaccattga aatactaggc tgtgatcagc aaaaatgaat ctgaataaaa aagagagcaa
     3481 ctattagctg agaggagtta atagttgtta gctttatacg aatcaaagtg gcaaatacag
     3541 atgcgtatgc atttgtgttt gccatcttgc gtttggtaga cgagataggt ataagctaaa
     3601 gaaacaactt gtttaaaata aacgacctag ctcaacaaat gaccatctaa tcaactaaca
     3661 gcatacataa aagcaactat ttttgaagta gaattgaaat cgggataact taaaaagtga
     3721 ttgggatact agtgagaatc accgtacata aatattactc gttaagggaa attgagaaat
     3781 tttgtatgaa aatttaagaa ttagttagta tgcgcaagtc aaaaatatga ataaattatt
     3841 tggtggagat aatttgctta gcttgtcata agaaatgaga taatctggca gatgcaagct
     3901 tttggacacc atttattcgg aataaggagg ctgggacaga agcgtttaac tccaagaaat
     3961 aagaaggagt tgcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt
     4021 tctttacttt tgtcccacgc tccttatttt tttattttgt ttggatcaat tcttttaatt
     4081 cctctattga aatagattca tcaaagattt ctttacccaa gatgacagtt ggaacaaatc
     4141 gaatatgtgc ttgctcagct tcgttgatga cagcttggct cagctgttcg tctttttgtt
     4201 ctgtcagctt tagtttatct actgcaaaat ccgctacttc ttggagagaa agatgtttcc
     4261 actcgtcttg agtatcgaat atttgtttga tctctttgat cgctttttgg ccatcgctga
     4321 ttgtgagata ccggtgcatc acgttccctc gctgtaagct ttctttttct ttatcgaaca
     4381 atttgatcac tcgctgcaat tgcccgcttt ggactgcttc ttctaatagc tcataagatt
     4441 cttcaaacca ttgtcggcaa tacggacaag ctaagttgat gaattcgacc atttttttag
     4501 gagcattcga tgaaccataa aggattcctt tttgtgtatt tgttttcgtt gcatcaatca
     4561 ctgatatatc catgttaaaa cacctctttc gcttatagtt tatacatgtc tgacaaaaaa
     4621 aggcaacgat ttagactgtt tgtctaaaaa ttagatgacc aattcaaata gatacatgta
     4681 aaatattttt ataaataaaa gaaaaaaatg gcatgtttta cgttataatg gaacagataa
     4741 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
     4801 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
     4861 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaacgt
     4921 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
     4981 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttcac aagggctgct
     5041 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccacgt
     5101 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
     5161 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
     5221 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtcccttttt tgaatttctt
     5281 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
     5341 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
     5401 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
     5461 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
     5521 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
     5581 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
     5641 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
     5701 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
     5761 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
     5821 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
     5881 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
     5941 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
     6001 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
     6061 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
     6121 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
     6181 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
     6241 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
     6301 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
     6361 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
     6421 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
     6481 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
     6541 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
     6601 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
     6661 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
     6721 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
     6781 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
     6841 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
     6901 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
     6961 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
     7021 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
     7081 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
     7141 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
     7201 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
     7261 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
     7321 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
     7381 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
     7441 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
     7501 ggaagactgg ttagtacaca tgtttgagag tgaaggaacg cagcaaagag cagcaaaact
     7561 gcaagctgcg atcgaaagcg aacgatcaat tttccctgaa ctggaggata tatacaagag
     7621 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
     7681 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atatccttgt
     7741 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
     7801 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
     7861 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
     7921 aacgatcaag gcatttgaag aagcagaaaa gtatcctggt ccttctctga ttattgccta
     7981 tacgccttgt atcacacatg gcttgcttgg cggaatgaaa gaatcgctta aagaagcaaa
     8041 agaagctgtt tcttctggtt attggtcatt atatcgatat aaccctacaa tggtcgaaaa
     8101 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
     8161 catgaggaaa caagttcgct tttcctcgct agataacgca aatccaaatc ttgcaaaagc
     8221 tttatttgaa aagactgtcc acgacgctaa aacacgtttt tacaactatg ctgcactgac
     8281 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
     8341 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgctagaag
     8401 agctgccaga gcagaacgac gtaaaaaaat ggaacaagct acaaaagaaa aaaaggaaaa
     8461 ataacagaaa aaatataatg aataattctc tgaagtttaa aaatatttat tgcagagaaa
     8521 aataaaacgc atacttgcaa ttttcttgaa aattatgcgt ttttctcaag aaaaatacga
     8581 gcatatcagc ctctaaaatt aaaacatatg atatactaga gaggaaatct caaaagagaa
     8641 gagaggcttt ttatgtcatt tcaaatcggg gaacttttta atttggacta ttggcagcaa
     8701 atgttttccg gtgacttgtt ctctctgaat tttttggtga acatacttga tattctggtt
     8761 gtctggtatc ttgtatacaa actgatccaa ctgctgcgag gcacaaaagc gattcagctt
     8821 ctgaaaggtg tagctatttt tattgttatc cgaatcgtag cggaaatcat tggtctgcac
     8881 accttgtctt ggctgatgaa ccaagtcatt acgtacgggg tgatagctgc tatcgtgatt
     8941 tttcagccgg aaatccggag aggactggaa catcttggac gcagttcgct tttcaaacaa
     9001 tcaaaaagcg aacagcagga agatgagaag atgatcttgt cttttgacaa agccatccaa
     9061 tatatgtcca agcgaaaaat cggtgcgttg atcactatcg aacgtcatac tggattagat
     9121 gagtatattg agacagggat tgctttagat gcagatatca ctggggagct gctgatcaat
     9181 atctttattc ctaatacacc gctgcatgac ggtgcagtta ttgtaaaaga aggaaagatc
     9241 gcagtagcaa gcgcctatct tcctctatca gaaagtatgt tgatccctaa agaattcggg
     9301 actcgacacc gggcagctgt cggtataagc gaagtcagtg acgcgattac catcgttgtc
     9361 tctgaagaaa ccggggacgt cagcatcacg ttagataatg aactgatggc tggcttatcc
     9421 cagcaagaat atttggcaat cttgcgtaga gaactaattg ctgaagagaa aacaaaaaac
     9481 aagaaaaacg tgattcaatc tttcctagac ggtatgacta agggagggga tcgcaaatga
     9541 tctcaaaaga gaaacggaca agcattcttt acagttttat tgcattgctg ttcagtattg
     9601 cgctgtactt caatgcaaat ggccagtccg ttccaaatac gctttctgga aatgaagcgt
     9661 ataatcaaac agttacgggg gtgccgatca agatttctta tgattcaaag cgctactata
     9721 tccatggata tgaaaatgca gtgactgtca aactttccag tgcaaaccga gttcagttga
     9781 atgcagaggc aaacgaagat acacggatgt tccgcgtcac agcagatatc actaagctgg
     9841 gcgaaggaac acatgaagtt ccgctaaaaa ttcaaaattt gagcagtgct gtcacagcaa
     9901 agatcgaacc atcgactatt acagtaacgg ttgagaaaaa ggtaataaaa gatttcgatg
     9961 tggaaacgtt gattccttca acgttcacac catctggata tgaacttgat gatacttccg
    10021 ttagtcctaa aaaagtctct atcactacag gtgacaaaac attggcagaa atcaaacgag
    10081 tagtagctga tgtggacgct tcgatggtca cagatgatgg gattaatagt gaggtcccaa
    10141 tacaggcgct gaatgcagca ggggaagtat taagtatcgt ctctgatcca gtgcaagtaa
    10201 cagtgagagc tgattcgatc aagccaagca agtcggtacg actttatggg atacagcaag
    10261 gaacaccagc tgccggggta gaaagctatg atttcagttt ttcaaaactg gaagcagaag
    10321 taagcggctc tagcgatttg cttgcatcga tcggtgacag tattgctgta ccgatcaatg
    10381 tctcaggtat caatcatcga acaactcgta aaattgaaat accagtagaa gaggggttaa
    10441 gtatcaagcc cgaatcagtt agtgtggaga ttacacccgt gttgcaaacg tcaagcagta
    10501 gctctgcttc gacgagcagt cggacacaag aaaatacaag tacgagtaga agtatttcat
    10561 ctgctgattc aggaacggaa ccatcgacaa caaccatttc ttccagttcg gaatctggga
    10621 caagcagctc gaccacaagt acagaagaaa catcagaaac aagcgaatca acacaaatga
    10681 gcagaacaaa ttcctcagaa aattaagtta aaggagtaat tttacaacta tgggtaaata
    10741 ttttggaaca gacggcgtac gcggtgaagc caacaaggaa cttacgccag aacttgcatt
    10801 taagctggga cggtgcggag gttatgtcct aagccagcat gaagaagaag gaaaacgccc
    10861 acgcgtgtta gtagggcggg atacaagaat ctcaggtcaa atgttagaag aagcgctgat
    10921 ttcaggactt ttatcggtgg gtatcgaagt gttccagcta ggtgtgatct ctacaccagg
    10981 tgttgcttat ctgacacgtt tgcaaaaagc aagcgcaggg gtcatgattt ctgcttctca
    11041 taatccagca caagataatg gaatcaaatt tttcggtgca gatggtttta aactagtaga
    11101 tgaccaagag ctagaaattg aagcattatt agatgctgaa gtggatgaat tgcctcgtcc
    11161 atcagctgaa ggattgggaa ctgtagaaga atttccagaa ggcttattga aatattctca
    11221 attccttgta caaacgatca acggtgacct ttccggtttg accatttgtg tcgatgcagc
    11281 aaacggagca acttcaacaa gtgttaatcg tttgtttgcc gatcttgaaa cagattttta
    11341 tacaatgggc acaaatccag acgggttaaa tatcaatgac ggcgtaggct ctacccatcc
    11401 agaacgttta gctgagatgg tcgttgaaaa aggagcagat gctggactag ctttcgatgg
    11461 cgatggcgat cgtattattg cggtggatga acttggaaac attgtcgacg gtgataaaat
    11521 catgtttatc tgtgctaaat acttggcaga aaaaaatcga ttgaaaaaag atacgatcgt
    11581 tacgactgtc atgagtaact taggattcca caaagcggtg gaaggaatcg gtttgaaaga
    11641 tgtcgttaca caagttggtg atcgttacgt tgtagaagaa atgcgcaaaa acgattataa
    11701 ctttggcggc gagcagtctg ggcacatgat tttcttagat tttaacacaa ctggagatgg
    11761 gatgctttca ggaattcagt tactgaatat catgaaacag acaggtaaga aattatcaga
    11821 gcttgcagca gaggtaacaa tctatccgca aaaattagtg aatatccgcg taagtgataa
    11881 aaacggtgcg atggatgttc cggcaatcaa aaaagtgatt gaagaggcag aagctgaaat
    11941 gggatcagaa ggaagaatcc tagttcgtcc ttccggtaca gaaccattac ttcgagtgat
    12001 ggcagaagcg cctactgatg agaaagttaa ttactatgtc gataaaatcg cggcagtagt
    12061 caaagaagaa atcggactgg attaagagat attttcataa aaaataaaag aacaaggacc
    12121 agtgtcagat gtctgacact ggtccttgtt cttttattat tctactgtca ctgatttcgc
    12181 taaattacgt ggtttgtcaa cgtcgaatcc ccgtaatagc gttgcgtaat atgcaagtaa
    12241 ttgagtcggt acgactgtgg ttagagtagt tagataaggg tggacagttg gtagcaccag
    12301 ctggtcactt tccttttcta atccctctga gacaataact agtgttttag cgccacggct
    12361 ttcgacttct ttcaggtttc ctcgagtatg cgcaccagtc acttcttcac tgatgattcc
    12421 gatcactggt gttccttctt cgatcaaagc aatcgttcca tgtttcaatt cgccagcggc
    12481 aaaaccttct gcctggatat aactgatttc tttcaatttc aaagcagctt caagagaaac
    12541 gttgtagtca gcaccacgtc caatgtaaaa ggcatttctt gtatcgctga gatatacttc
    12601 tgccaactct tctaaataat ctttttgggc gatgaccgat tccatcgcat tagcaactaa
    12661 actcaattca tgagtgatat cgaatacaag agaagtttca aattcttttt tatcaccgat
    12721 agctttggct aataaagtta gtacagctat ttgtgcagta taagctttcg ttgatgctac
    12781 tgcgatttcg ggtccagcat gcaatagcag tgtaaaggat gcttcccgcg aaagagtaga
    12841 accagcaaca tttgtaatcg taagtgaagg gtagccgaga cggttgattt ttaccagcac
    12901 ttggcggctg tctgctgttt cgccactttg actcaagaaa ataaagaatg gtttttttga
    12961 aagaagaggt gtgttatagc caaattcact ggctaaatga acctcaacag gaatctgtgt
    13021 caatttttcc aaagtctgtt tacctgctaa ccccgcatgg taactagttc cgcaagcaac
    13081 gatatagatt cgatcgctat cagacaatgt atcgaccagc ttttgatcaa gagagacacg
    13141 attgtcttct gttacgtatt tttgagcgat acgacgcatg ataataggct gttcgtcgat
    13201 ttctttcagc atataaaaag gataggtacc tttctcgata tctgaagcat ctacttgtgc
    13261 ttcgaatgaa gaacgttcta cgatgtcacc tgcaaaattt tgaataataa ctttttctga
    13321 tgtgacggta accatctctt catcttcaat ctcaacgaat tccttcgtat gagcaagcat
    13381 agccattgca tcgctggcaa tcacattgaa tccatcgccg agtccgatta atagtggact
    13441 tttattttta gcgacgtaaa taacatcagg tgctgtccga tcaatcaaag cgaaagcgta
    13501 agaacctttg atgacccgca aagcttttaa aaacgcctct tgcgttgtag tatccttcgc
    13561 gaaagtttca attagatgag cgataatctc agtatcggtc tcaccaataa agtggtcatt
    13621 tgctaaataa gcaattttaa gttcttcaaa gttttcgatt acgccgttgt gaacgagcac
    13681 gaagcgtcca ctttgggaag tatgtggatg ggcgttttct tcagaaggtt gcccgtgagt
    13741 tgcccatcgt gtatgaccaa tacctatatt tcctgtagta tcaatattta ttttgttttt
    13801 caaattttga attctgcctt gtgctttgac cagataatca gttgtgccat ctgagacaaa
    13861 gataccagct gaatcataac cacggtattc taatttttct agaccgtcaa tcaatcctgg
    13921 tgttacattt tttaatccaa tcattccaac aattccgcac ataaaaaaca tcctactttc
    13981 tataatgaat tcgtatgaat gcttccagct gcaggaaaat ccttttgttt tatctttgca
    14041 gacatttttt gtggattttc tgtagagtgc agtccctagg gccatccgcc gaatctttcg
    14101 ataaaccctc tcctcgtcat ctgtttacac agacctggcg ctatcctaaa aggtataact
    14161 atcctccttt ttgtttttta tctggaaaca tttaaaatca taaccttttg cgaatgttct
    14221 tgtcaacaaa aaaagaataa aaaataaatt ggtatatatc aaattctttg tcaaatgtcc
    14281 tttttatgtc aaaaaataat gggtttttat ctatcatctt tttcaggagt tcggtacaat
    14341 agaaagtaat cggatagagg aggagtaaaa atggattcaa aaattgagat catgacactg
    14401 ggaatgttaa aaaaacaatt gtccaaattc gaagcatcag ctggtgtatc agatgatacg
    14461 aaaatatttt tagatactgg ctgggatagc atccaagaaa tcgcacctga tgccttggaa
    14521 gttgctcaag ctcgtgagtt tacggtagaa gatgaattaa caaaagagtc gttttccggg
    14581 tatgcgcggg aagaaaaagc agagcggttc gacacttctg agcagtcaga aactgtgatc
    14641 gtcataaaaa acttgtatta gaaaggagaa agaatgaata aaagacgttg ggcagcagtt
    14701 ctgattgctg caggattatt tgtggtttcg ttagtttcag ctagtttgac taagcctcag
    14761 gaaaacgatc aaattgaggg attgaacagt tggttatatg gggatgaaga actagctcca
    14821 aatgtgttgg aagagggaga cagtacgcaa aaaatcgtaa aattatcagt agatggaacg
    14881 attgctgcgg gagggtcttc aggtttattt gcttctgaag gttacaatca taaaaatttt
    14941 atggaacaac tgaaggcgat tgaagaagat ccaacagtca aaggtatctt tttagaagta
    15001 aattctcctg gtggcggtgt ctacgaaagt gcggaaatcg ctaagaaatt ggatacgatt
    15061 cgtaaagaac atgatatccc aatgtatgtc agcatgaaaa acatggcagc tagcggtggc
    15121 tactatattt ctgctcaagc agataaaatc tttgcaacag aagaaacagt gaccggctca
    15181 attggtgtga tcatgtcagg attgaattat tcaggacttc tagaaaaact gggtgtggaa
    15241 gatacaacag ttaaaagcgg agcgttgaaa gatatgggtt caggaacgcg accagagaca
    15301 aaagaagaag aagcggtgct tcaagcatat atcgacagcg catatagtcg ttttgtagca
    15361 atcgtcagcg agggacggaa taaatctgaa gaagaagtca aaaaaattgc tgatggaagg
    15421 atttatgatg gtgtccaagc aaaagaagta ggtttagtcg atgaattggg atttcctgaa
    15481 gatgctttaa aagctatacg aaaggaacaa cagcttgaag atgcggaact agtagaatat
    15541 tcttccacgc catctgggtt tgctagtaca tggttaggaa gtaaactagc agaattccag
    15601 ggattgaagc cttctgagac tagtcagatc ttatccgttc ttgagaatct agggacagcg
    15661 gaatcaccaa aagcaatgta ttattacgga ggtgagtaag catgtcagag ctttcaaatg
    15721 aaaataagga acaagaaaaa gataagcaac agcagcctgc tgtaggacct gactccaact
    15781 tttcccaaaa ggtactgagc tcttttgaaa gagagccttt gacgcccgaa gaaatcaaaa
    15841 agaaacaacg taaatggaaa aagtatacgg acacaagaaa tgaaccgcca aaagcaaatg
    15901 attttcctaa gtatttctat gcaggttttt ttatccgaat gtttgccttc ttggtcgatc
    15961 ttttgtgtat cggagctatt acgcgtttaa cacttggtat tgccaaaaat ctaggttggc
    16021 ttaatttttc agattcttat ctgagtctct acggattcac agccttactt atttatctag
    16081 cgtattttat tttattgacg aaattcaatc atggccaaac gattgggaaa atgatctttg
    16141 gtattcgagt catcagcttt gatgaagaag aacttagctg gtcaaccgtt ttagttagag
    16201 aaggtgcttg ccggttcatt ctaaaatttc ctttgctgat gttagggtat cttccagcag
    16261 tctttaatca gaaaaagcaa catctgggcg attttttcac taatacaagt gttgttacga
    16321 tcaatctgat caaagcgttc aaccaggaag tcaacagctg atgagcaata aaaagcagta
    16381 acagttgaaa gcaccttcta aaagtcagat ctaaaatcta gcttttagag ggtgcttcag
    16441 ttatttttaa ttgtcatgct ttttttgttt tgctactatt gaacatgagc tgaatatgag
    16501 caagaggagg acactgcgtg aagccgattg attttccaga aaaatatgag aatttaatgc
    16561 gagtggcaca acaagcacta gcaaaccagc aatatcagca ggcaaaagaa ttatttcaac
    16621 gagcttacga gttgaaagaa agctttgagg ctaatagcct gcttgttttt tgtctttacg
    16681 agttggacga gaaaaaagaa gcattgaagc aagcactctt gcatgaaaaa cagtatcttc
    16741 aaaatgagga gtttgctgaa ttttattttg acttgttaat cagtacacaa gattttttat
    16801 atgctagaaa attgatagca agtacagatt tttatgaagc tttcgagcag cgggtcattg
    16861 aaaaaatcca atttgcagaa gaattatccg gacaaatgga gaggcagaag atcaaggcac
    16921 tccacaataa atcggaggaa cttccttcgt tggaacctgc acgtcagctt gcgtctattg
    16981 atgagatcga acagcttcct tatcatgagt ttatccaaac agcgagtaaa cttattgtat
    17041 cgccagaggt ccatatttta gcaagggcaa aattactgga gacacttagg caattgaacg
    17101 aatgccatcc tgttttctat ttgacgattg aagaaaagtt agtcaaagtc ataccaaaag
    17161 atctgccaaa gccgcagcag caggcaagtt accagcaatt atgtgtattt tcagatcgtt
    17221 acgggaatga agatgcgctg cttagttccg tgttaaaaga agaattcacg ttgcaatctg
    17281 caattgttta tcctgtctat gatacataca tcgaagaccc gaagcgctgg tttcaactga
    17341 ctgtagaggc gtgtacagga aaaacgatgt atgaaggtac agaagatgaa aaacaagatt
    17401 ttttaaaaaa aagagaaaaa attttgcagc aaatgatctt cttccattag attttatcag
    17461 tgaaaaagat aaaaatgtta tgaaaaacaa ggattctatc cattcaatat gtttacaatt
    17521 tgaaagggtt agtgtactat ttaaaggtat gcatgaaacg tttttgatgt ataaaatgaa
    17581 tttctaaact cggagggaat taaatgactg caacatttga aaaaaaaggc acgaatgatg
    17641 gtgttttaaa atttacgatt gcgcaagacg aaatccaaaa aggattaaca actgcgttta
    17701 ataaagtgaa aaaaagcttg aacgtacctg gattccgtaa agggaaagtt tctcgtcaag
    17761 tattcaaccg tatgtacggt gaagaagctt tatatgaaga tgcattgaat gctgtattac
    17821 cagaagctta tgaagctgct gtcaaagaag ctggcatcga tcctgtttct caaccaaaaa
    17881 tcgatgttga aagcatggaa aaaggtcaag actgggtaat cactgctgaa gtaacagtga
    17941 aaccagaagt aaaattagga caatataaag atctagttgt tgaaaaacaa gaccgtgaag
    18001 taacagatgc tgacgtggat gcacgtatca aacgtgaaca agaagcacaa gctgaattag
    18061 ttatcaaaga agatgctgca gctgaaaatg gcgatacagt agtaatcgac ttcgaaggct
    18121 ttgtagatgg cgaagcgttt gatggcggaa aaggcgaaaa ttactcactt gaattaggtt
    18181 caggctcatt cattccaggt tttgaagatc aattagttgg tcataaatca ggtgaaagtc
    18241 tagatgtaaa tgtaactttc ccagaagatt atcaagcaga agatcttgct ggaaaagaag
    18301 cagtcttcaa agtgacaatc catgaagtaa aagcaaaaga agtgcctgaa ttagatgacg
    18361 aattcgcaaa agatgttgat gacgaagtat caacattaga cgaattgaaa gaaaaatatc
    18421 gcaaagaatt agtagaaaca aaagaaaaag cagcggacga tgcaaaagat gaagcagcga
    18481 tccgtatggc tgttgaaaac gcagaaatcg ttgaattgcc acacgtgatg gttcatgatg
    18541 aagtacatcg ttcaatggac gaattcttga acaacatgca acgtcaaggt atctctcctg
    18601 aaatgtacta tcaattaact ggttctacag aagaagactt gcataaacaa ttcgaaggtg
    18661 aagcagaaat gcgcacaaga acaaatcttg tgatcgaagc aattgcagct gctgaaaatc
    18721 ttcaagcaac agaagaagaa atcaatgcag aaatccaaga attatcagaa acatacaaca
    18781 tgccaatcga acaaatcaaa cgcgtgttga cagaagatat gctggaacat gatgtgacaa
    18841 tgaaaaaagc aatcgaattg atcacaggta cagcgacaga agcgaaagaa tagaaatcaa
    18901 gtaataaatc atttctgaag aagctacttc tagaaaattg tttattcgaa ataagggact
    18961 gtgacacgat tttgtcacag ttccttattt tttgttaaga gtaaaaaatg caaaggtata
    19021 ggtcagttga tcctttgtta aacacttttt ctttttacta aactatgtta tgatagtaaa
    19081 agactgtaaa gtctttgata gtttcttgca tactttttgc aagttaaggg gtgaagacca
    19141 tgtatgacaa tccaagtagc aatgagaccg tacgctgttc tttttgcggg aaaacccaag
    19201 aagaagtgaa aaagatcgta gctggtccag gggtctacat ctgtaatgaa tgtatcgacc
    19261 tatgtaaaga aatcattgat gaagaatttt atgaagaagc tgtacgcgaa ttcactgaag
    19321 tccctaaacc actagaaatt cttgaagtat ttaataacta cgtgattgga caagatcgcg
    19381 caaaacgctc gttagctgtt gccgtataca atcactataa acgtgtcaat cagcaagcac
    19441 aagaaaacac agatgatgtt gaactgcaaa aaagcaatat ctgcttgatc ggacctacag
    19501 ggtctggtaa aacattttta gcacaaacat tagcaaaaac attgaatgta ccttttgcaa
    19561 tcgcagatgc gacaagtttg acagaagctg gttatgtggg ggaagatgta gaaaacattt
    19621 tgcttaaatt gcttcaatct gctgattaca atgtggaact tgcagaaaaa gggatcatct
    19681 atattgatga gatcgataaa attgcgcgta aaagcgaaaa tgtatctatt actcgtgacg
    19741 tttccggcga aggtgtccaa caagccttgc tgaaaattct agaaggaact gttgcaagtg
    19801 tgccacctca gggcggacgt aaacatccac atcaagaatt tattcagatt gatacgacga
    19861 acatattgtt catcgtaggt ggtgcatttg atggcatcga aacgattgtg aagaatcgta
    19921 tgggcgaaaa gacgattgga tttggtacac aaaaccaaaa actttctgaa gacgaaagtg
    19981 tcatgcagca tatcattcct gaagatttac taaaattcgg attgatccct gagtttatcg
    20041 gacgattacc tgttatggca gctttagaga aattgacaac agatgatctg gtacggatct
    20101 tgactgagcc gaaaaatgcg ctagtcaaac aatatcaaaa acttctttct ctagataata
    20161 ccgaacttga attcgaacct gaagcgttga gagccattgc gaaaaaagca atcgaacgca
    20221 ataccggtgc tcgaggactt cgttccatca ttgaagaaat catgatggat gtgatgtttg
    20281 atatcccttc aaatgaaaac atcgaaaaag tgattatcac aaaagaagca gctgaattgt
    20341 ctggtgagcc aacagtaatc tataaagatt cagagaaaaa agcgggttga tgaaaagagg
    20401 ctgccgtaaa agtcattttg gcttttgcga cagccgcttg ctttattcat caccttgtta
    20461 aggagagaga ccatgaaagt ccatcaagct gaaattatca taagtgcagt ggcaccaaaa
    20521 caatatccag atacacaatt gccagaaata gctctggcag gacgatcaaa tgtagggaaa
    20581 tcttctttta tcaacacatt gattgatcga aagaatttag cccgtacttc cggtaagcca
    20641 ggaaagaccc agacattgaa tttttatttg atcgagaacg cattacattt tgtcgatgtt
    20701 ccgggatatg ggtatgcaaa agtatccaaa actgagcgag caaaatgggg aaagatgatc
    20761 gagacatatt taacgactag agaccagcta cgtgcggtcg tttctttagt agatttgcgt
    20821 cataagcctt ctcaagaaga tatccagatg tatcaatttt tgaagtatta tgatatccct
    20881 gtaattgttg ttgccactaa ggcagataag atccctcgtg gtaaatggaa caagcatgag
    20941 agcgaaatca aaaaagcgtt ggattttgat cgcaatgaca cgtttattct cttttcttcc
    21001 gttacaaaac agggaaaaga agaagcgtgg gcagcaatcg aacaagcaat tgaatcataa
    21061 aaagaataaa aaagacttac cagtcaaact ctttcataaa agtttggctg gtaagtcttt
    21121 ttttgagctt aacttacttt tgtttcttgt cttttttctt ttcattttgt ggctgtggtt
    21181 ttagaaatcg atcgatctct tcttttggtg caactgtttt ttccggatcg acagcaaatg
    21241 attcgaaaag tttatctaaa ttatctgtac gttcaaattt caaccccatt gtaaacacct
    21301 ctttgtttct ttttacttag tctatcatgt aatcgtccaa attggtttct aagacataaa
    21361 gttttagttt ttctttatca ttggttataa ttaaattata aaaagatttg aaagaggtga
    21421 gaaaatgatt ggcgaagata aattattgat gactatccag ctcacagact taaatgaaga
    21481 agaaaaaaaa caggaaataa gcgattgggc tattttgaca cgtctgttga tccagccaac
    21541 atttatccgc tctttcacac aacaagcgga tccgtatgat cttcgaaagc ttcaagaaaa
    21601 aataatgcta gcatcggtaa gagacgaaag ttggttagtt gtaggaaatg acgagaacga
    21661 atgtagtttt cgcttggaag ataatcaatt attgataaaa aatgtattgt ctatctctgt
    21721 tttcaaagaa aagcaatttt tgattcgaga cttcattcag aaaaaaatga ttgctcatgg
    21781 ggtgttcgcc tatatgcgag cttatagcga gtttatctat cacaatacaa aacagatctc
    21841 tcaacggcta ttgtttgaga aaaaggatga aatcgaacat ttacctaaaa tgaaacaaca
    21901 aaacggggaa gtagtagtcg attgcaacca atttccagga tacgatcttt tttaccaagg
    21961 tttatgtttc acttcgtgtt gggaaatgta ttatagtcgc tattatcatc aaatcatccc
    22021 taaaccgatt tttctcgaca tccaacaagt agaaaaagtg aaggaattag aaaacgaagt
    22081 catatgtatc cagctttatc gagacccctt taactggcaa aagccaaaca atcaaatgtt
    22141 tcaatcctat tttagggacc agcttgggtt tgatcatctt gcttgggaca atggtgtcgg
    22201 tttattgaaa ccgccattcg ttgaatacat ttacactgac catacgatcc aatctgtcca
    22261 gtatcagaat gcacaaatgc aacctgttcc taaaaagaat gcttcatttt ttgtaacaaa
    22321 aagttatgat atccaacaag gagattataa agaaaggcgt gttcgcggaa cactaaatgc
    22381 tcaggcgtat tttccttggg ttgacgagaa tcgatcacgc atgatgtgct ataaagttat
    22441 cgatccgact gttgctttgg ataatggaat cgaagcttat tgttattata ttagggaata
    22501 tctagaagta gaggtaacag atgaaaaata tcaagagtat cttttatcct taagaatcta
    22561 tgttccttca gaatccctgt cagaattacc gttaaaggaa atcaaacata gactctctga
    22621 tgttaccttc aaacgaataa agaaaaagcg gcgaagtatc caattcgatg tcaagaaagg
    22681 tgagcagcat ctgcgtgtgc aatttttaga ttaccgagaa ttagaacaat tgattacttt
    22741 acaaaaaata tgaggtgaag aaaatggata aacaactcta ttatcgaata atggttccgg
    22801 catcattatt atccgtctgt ctcttttttg ttcctaattt tcctattcga tcagtcatcg
    22861 gttttttggt catgtttggc gggatgggac tttattatta tcttcttcat aaggaaagaa
    22921 aacctgcttc tgtttatatg aaaaaaacaa gaagcgaaaa taaaaaacga taaaacggct
    22981 tgatgataga caaaaaaata aaaa
//
LOCUS       DACDSR010000037        22835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDSR010000037 DACDSR010000000
VERSION     DACDSR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.037"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(353..673)
                     /locus_tag="IWK15_001473"
     CDS             complement(353..673)
                     /locus_tag="IWK15_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547071.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1176..2294)
                     /locus_tag="IWK15_001474"
     CDS             complement(1176..2294)
                     /locus_tag="IWK15_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547072.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2765..4072
                     /locus_tag="IWK15_001475"
     CDS             2765..4072
                     /locus_tag="IWK15_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9547073.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4193..5392
                     /locus_tag="IWK15_001476"
     CDS             4193..5392
                     /locus_tag="IWK15_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9547074.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5419..6687)
                     /locus_tag="IWK15_001477"
     CDS             complement(5419..6687)
                     /locus_tag="IWK15_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9547075.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(6857..7498)
                     /locus_tag="IWK15_001478"
     CDS             complement(6857..7498)
                     /locus_tag="IWK15_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9547076.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(7907..8410)
                     /locus_tag="IWK15_001479"
     CDS             complement(7907..8410)
                     /locus_tag="IWK15_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9547077.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8533..9297)
                     /locus_tag="IWK15_001480"
     CDS             complement(8533..9297)
                     /locus_tag="IWK15_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9547078.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9405..9680
                     /locus_tag="IWK15_001481"
     CDS             9405..9680
                     /locus_tag="IWK15_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9547079.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10240..11190
                     /gene="yidC"
                     /locus_tag="IWK15_001482"
     CDS             10240..11190
                     /gene="yidC"
                     /locus_tag="IWK15_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9547080.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11236..11637)
                     /locus_tag="IWK15_001483"
     CDS             complement(11236..11637)
                     /locus_tag="IWK15_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9547081.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11651..12460)
                     /locus_tag="IWK15_001484"
     CDS             complement(11651..12460)
                     /locus_tag="IWK15_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9547082.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12447..13337)
                     /locus_tag="IWK15_001485"
     CDS             complement(12447..13337)
                     /locus_tag="IWK15_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9547083.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13350..13829)
                     /locus_tag="IWK15_001486"
                     /pseudo
     CDS             complement(13350..13829)
                     /locus_tag="IWK15_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(14005..14946)
                     /locus_tag="IWK15_001487"
     CDS             complement(14005..14946)
                     /locus_tag="IWK15_001487"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP9547084.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(14943..15938)
                     /gene="lacD"
                     /locus_tag="IWK15_001488"
     CDS             complement(14943..15938)
                     /gene="lacD"
                     /locus_tag="IWK15_001488"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9547085.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(15931..17127)
                     /locus_tag="IWK15_001489"
     CDS             complement(15931..17127)
                     /locus_tag="IWK15_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9547086.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(17145..17873)
                     /locus_tag="IWK15_001490"
     CDS             complement(17145..17873)
                     /locus_tag="IWK15_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9547087.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(18314..19288)
                     /gene="lacD"
                     /locus_tag="IWK15_001491"
     CDS             complement(18314..19288)
                     /gene="lacD"
                     /locus_tag="IWK15_001491"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9547088.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(19285..19743)
                     /locus_tag="IWK15_001492"
     CDS             complement(19285..19743)
                     /locus_tag="IWK15_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9547089.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(19740..21203)
                     /locus_tag="IWK15_001493"
     CDS             complement(19740..21203)
                     /locus_tag="IWK15_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9547090.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(21185..22117)
                     /gene="pfkB"
                     /locus_tag="IWK15_001494"
     CDS             complement(21185..22117)
                     /gene="pfkB"
                     /locus_tag="IWK15_001494"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9547091.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            22259..>22835
                     /locus_tag="IWK15_001495"
     CDS             22259..>22835
                     /locus_tag="IWK15_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9547092.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6869 a         4897 c         3832 g         7237 t
ORIGIN      
        1 acaaaaacta ctcctatagt atatcatacc gtttacaaaa aagctagagt ttttcgactt
       61 tttttattaa aaaaagtttt ctcagcgatt acataaagtg aaaaaccgaa ctttttttta
      121 aaataaacga catattgttc agttggtgtt tttttgctct gatgttcttt tttaaactaa
      181 aaaaattatt ctttctgact acttatcggt tctgtttcaa cggtcgaggc tggggaagat
      241 tctttagtag gtctgatgtt ctcatggtat ttctgatagc ctatgacagt taccagaaga
      301 acaaggcaaa ccaatacgac caacgagatt agtttttctt ttttgctcat atctatacct
      361 cataaataac aggatgatgt ccatcatatt tctgagtatg agtaaaagca aaagctgcag
      421 ctgtagaatg taagaaatgc cagcaacttt cataatcaca ggtcggacag taccaaaagt
      481 aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg aatcgcagaa tcaagatctg
      541 gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt aataggaaac cgctgtgttt
      601 cttcagcaaa aactggctga cttgttaaac ttagggatag agacagcaaa caccataaca
      661 gtcttttttt cataaagacc tccttatcga taagtttttc ccaattaaaa tcatatctca
      721 agaacgcccg ttattcacat tttgttcata aatgatgttt tttgtatcat aatcgtcaaa
      781 aagaggtcgt gaccacgtca caacctcttt tttaaacggt gttccagaag tagcttcttc
      841 ggaaataagc cgaaatttca caaaaatttg agaaataatt tttgtcaatc tcttcttatt
      901 tcttgaagct gaccacttct gtcacaacct cttccttaaa cggtgttcaa aagcttgcat
      961 ctgcggtaat aagcccacca aacaaaaaat atgaagagct atttttgctt gctgttctta
     1021 ttacttgatg cagaacagct ttttcacaac ctctcataca cggtgtttaa tcagctgacc
     1081 ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta tttttccgtt tgtttctcta
     1141 atactcaggt caaaacactg atttcacaac ctcttttaat aacgaagaat cgctgtcaga
     1201 ctcttttcat ctggcgcatc tttttgatcc aaaataaaca cttttccgcc attaatgatc
     1261 acatcatctg ctaatgcatt caacacttga cgccgatcat attctgtttc aggatcttca
     1321 ccgaaaccat cagctaagtt tgacgtagcg ataaataaat gtgacacttt tccttctttc
     1381 gcagctggaa cgatatcaac taactgatcg atgaatttct tatctaagag tttattgtat
     1441 tcttttgatt cttgagctgc gagttcactt gacagcttct tcgccccttc ttcgatatct
     1501 ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt ctgcacaata atagggattt
     1561 ttagcaattt ttttgaacat cgtctgattt tctggtagag caaataaata aatcggccat
     1621 tgttctggat tttcgaaaga atccttcaag tacgtatcaa ctgcttggta atagttgacc
     1681 caatcgatct ctacttcttc atcttttgga tttacgccat ggaaagttac tccatctcgg
     1741 gctccgcctc ctccattaga agagaagttc aaaccgccac ctgtcagttc agatcccaat
     1801 gcagtaacga catcaaccgg tgccccttct ggtagagata cttcttttac ttgtttgttg
     1861 ctgacttcat acaatttcat ggagtcgcgg ttcaatccca ataaataata agaatagttg
     1921 aactgcccat ttttgataat agccagtaaa taaggaagat cacttacata gtattgatcg
     1981 tctaccggga tactcaaacg atggatatag gtttggtcag cagttataat gaccgctaca
     2041 ctcgtagttg cactacgcca aaacgaagga tctcctaaca aattttcaat tttttcttgg
     2101 aacggggacc agtcatgttc ggaatatttt ttctcaaaac gtttctttgc ttctttcgca
     2161 aaatttttga atttgatctg atctttttcc acatcttgat gggcgacatg cgtattcagc
     2221 atgatcgtga cgaatggtcc tttagcgcct tctgaagtca attgtgacaa taattcttta
     2281 tccttttttg ccataaacgt acccctccta aaaaaatcat tcctttgttt ctatctaaag
     2341 tctaccacaa cactattttt ccatacaata aaacgtatct aaaaaaataa aacggtttct
     2401 acggttttaa atttagctat ttttttaagt tatcttcata atttcctcaa ttatttagcc
     2461 ccagtcttca actattcttc atgaaatgct gataaactaa gaaaaagaat aaaagaggtg
     2521 gatcttatgc atttgattat gattttcagt ttattaagta ttatcagttt atttgctttc
     2581 agcgcttccc gcttattaga tgcggaaaca cagcctattc ctgtaaaagt acgtaaaaac
     2641 gaccgctaac cttccataac gtgcgttctt atcctaaagt tttttcagaa acattgaaac
     2701 tctctcccct ctctgataaa attttaattt gtagcgaaac aaattaatta caaggagatc
     2761 ggatatgtcc ataaaaaaaa cacgtctagt cctattattg gctagtctca ttctttgcct
     2821 tgcttctttt ctaccagctg ttgctgtgca agctgaagat actttcaaag taaatgcaaa
     2881 agcagccttt gctgtagatg cgcaaagcgg taagattctt tatgatcaag atggagaaaa
     2941 aaccatgggg atcgcttcaa tcacaaaaat catcggtctt tacatcgtcc ttgatcaagt
     3001 aaaagaaggg aaactatcat gggatgataa agtttcaatt tcagattatg cagaaaattt
     3061 gagtgtcacc ccagatttat caaatgttcc tttgcataaa gaaaacacct acaccgttaa
     3121 agaattattc gactctgcaa tcatccagtc agccaacgct tcaatggttg ccttggcaga
     3181 aaaaatctct ggcagcgaag caaaattcac tgaacgaatg aaagaacaat tgaaagattg
     3241 gggtatcaaa gatgcaacga tcgttaatgc atcaggactg aataactctt atcttggcga
     3301 aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa atgtctgctc aagatgtggc
     3361 tatcgttgcc cgtcatttga tcttggattt tccagaaata ctggatgtat ctagcactac
     3421 gacacaaatg tttggcgaaa acacgcaatc accagtagaa atggtcaact ggaactggat
     3481 gctcccagga ttcgtgaatt ataaagaagg cgtggacgga ttaaaaactg gtacgactga
     3541 acttgcagga gcctgtttcg tcggtacgat cactaaaaac gatcaacgaa tcatcactgt
     3601 cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc cgtttcaatg aaacagctcg
     3661 cttaatggat tattgctacg acaattggtc agaaaaagag cttggaaaag ccaatgcaag
     3721 tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa gaatcaacag tgcccgttgt
     3781 gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg gatactggaa aattaacaat
     3841 cacaccaact cttgacaaaa aacaagtcac tgacaatgag atccaagcac ctatggataa
     3901 aggaacaaaa atcggtaccg caacgatcac cttggcagaa gataaattag gttatcttga
     3961 agacaacaac gcgccatcca cagaaatcat cacggataaa accgtagaaa aagcaaatat
     4021 tttcgttatt ggctggcgac gcgtcgcaga atttttcggt aacttgtttt aattgaacaa
     4081 aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg ctttttctag tttaaataac
     4141 agcttttttc atctattttc gttatactgt ataagaaaaa ggagttgaat ttatgaatct
     4201 aaccagagat ctgatttttt tggttctcta tcttattggg gtattctcca tcgtttattg
     4261 gcgaaaacag aaaaaggatc cttccttgtt cttttacagc agtacatgga cgtttgcggt
     4321 aatcttgact tattttgtcg tgttggaaat tttttattcg accttttatt ttttgattcc
     4381 cttagtgttt ttcagtatct ttgccttttc ctatttttca gaaaagcgaa ggctgttgaa
     4441 tggtctttta ttcaatattt tcctcattag ttttggtatc tacttgtttg tcttgctata
     4501 cgaaacacaa aatatttttc ttggcggact gattgccttg atcacgatcc cgttactgct
     4561 tgtctttcta ttcggcattt atggattgat cgtttttctt ttttggaacg gtgtcacagt
     4621 tttacgtaga gagtcgcatt cattagctaa tcttttaacg ttgatactag ccatttttct
     4681 tacattgttt cttgtttttg actttttctt gttaaaatat ttaccacaat ggataaatgc
     4741 tttgtttttc tgcatcccat tgatcttgat ctatctattt atcgtattct acaacttttt
     4801 gactgtctca ttcttgtatc agttcaaccg ccctcgatac aaccaagact tcatcgttgt
     4861 gttaggcgcc gggttgatca atggagaaac agtttctcct cttcttgcca agcgaatcaa
     4921 taaggcgatt gctttttatt gggcacaaag ccgtgcgaca ttaaatccgc ctatcctatt
     4981 aatgtcgggt ggacagggag cagacgaaaa agtaccagaa gcaatggcga tgaaacaata
     5041 tgccatggaa caaggaattc ctgaaagaga tattcttgtc gaaaccaatt cgacaaccac
     5101 tttggagaat atgcgctatt caaaggaaat catggatcag caaatgaaag gtccctatcg
     5161 cgcgatcttc agttccaaca attaccacat tttcagagct ggtctctatg cacgacaagc
     5221 gaaactgaag gcaaatggca ttggggcaaa aacagctttc tattacttgc ctaacgcgtt
     5281 tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa agacatatga tcatttgcgg
     5341 attgcttttc gcaggttccg cattcttatc actgatcact ttacttttct aaaaaaacaa
     5401 ggtgtgacaa aacatatgtc acaccttgtt tttttctttt attctcttga cgattggcaa
     5461 gaatagtatt ccttttttga cggctacttg ctgtgcagaa taaataccgc tgtttcctga
     5521 acacataaag ctgatcaagc agatcataaa gaagtaagca gcagcttgtg aaccaaataa
     5581 ttcgattccc atcacaaaac aggcaatcgg tgtatttgtc gcaccagaaa ataccccgat
     5641 aaaacctaat ccagcaagaa aaggaacaga aacatggagc aaaggcgcta gtactgcgcc
     5701 aagcgttgca ccaatctcaa atagtggtgt cacttctccc ccttggtacc ccgcacctaa
     5761 agacaagacc gtgaagaata atttcccaat aaaatcataa gcatgtgcct gtccattaaa
     5821 tgattcctgc agtaacggaa gactcaaccc aaggtaacgt tgtgtttgca aaatcaacgc
     5881 tgcggctaca actactgctc cacctatcgt atttcgccaa acaggatgag gaatccattg
     5941 cgcataccgc gcttttaaaa agacgatcga acggctgaat acccaaccaa tcaaaccaaa
     6001 agcaatccca gcaacaaaaa ctttgaagaa cagcaacata ctccattcag gaatcacacc
     6061 catttggtaa tgtagatgag aaacaccata gctttctgtc atgaaatttg caaaaagcgc
     6121 agcaaagaag ctaggaaata ttgcttctgt tcggacttta ccgattgcca acacttctaa
     6181 tccaaataat gttccagcta aaggtgtgcc aaatacagaa ctaaaacccg cactgattcc
     6241 actgataacc aaaatctccc gttctgcttt atccaatcgg aataaatgcg ctatattatc
     6301 agctaatgct ccccccattt gaactgctgt tccttcacga ccaacagatc ccccaaataa
     6361 atgggtagtg atggtcccaa acaaggtcaa tggaatgagc cgcaaaggga tcttctcttc
     6421 tcctccattc ccttgatcga tcactaagtt attcccacga ctagaaagac cgccatgata
     6481 cgcataaaga taagcaaaaa gcgcgccgct gactggcaat agataaagta gccaaggatg
     6541 tgctagtcgg atatctgtca cgaacgtcag gctatttaag aaaaaagcag acaagctccc
     6601 catgaatact ccgagaatcg ctgtgaccac cagccacttc aacatgtaca tcaaaatacg
     6661 cactggactc ggtaattgtt tttccataaa aaattcctcc tcaaataaaa aaacgcctac
     6721 taaagaaagg ggctggcccc agactttagt aggcgtcatt agctatctta cgttagcatt
     6781 taaaggcgaa caccatcacc tattcacttc cattaaaaga tagcatattt ttctgtaaaa
     6841 aacaataaaa ttatcgttat ttctttacac gttcagcaaa ttcatagaaa ggagccaatt
     6901 tcgttaactg ttgattacat tgatcactat catgacaaat ataattcccc tttttcgtat
     6961 acgtgccatc agaaccggct ttcgttgtag aaagaaagag cgagacattc gatgttttct
     7021 gacagatcgc acagattccc ttattaatcg ttggtgataa agtaccgctg atccctttta
     7081 attttccatc ttcgtaataa accatgaatt ttttttgcgt ccctggatca ttccaaccga
     7141 tgtacgtatg tgcttgcaag tcgatttcat tccaagcggg ttgtttgagt tttttcactt
     7201 ttcggaataa tttctcgatt tgtggtttgc tcggctgcat aaacggctgg acgatttgct
     7261 tcagctcctc caatgctcgt tctgcttgcg tttttgtcag ttttacatct aaaactgtcg
     7321 ttaaaaacgt atcgatttct gatggttcgt aacctaatac atcgcgaatc ttttcagctg
     7381 ctagagcctg aaccgtttgt accgtttttg agtcattgac agatgagtac gcatttaata
     7441 actgctctac ttctcttttt ataaaaaaat attgatatgg tttcattgtt tgttccatga
     7501 ttttaaagtc ctttcttcta accaaagtgc atactttgta gaaaaaagtg atcttctgca
     7561 aagtatgtgt tacctgaaga tcccggactc ttttaaataa gatacgtttt gcatcttatc
     7621 atcaccccct ttaatgttca ttcttctatg tacatgattc attcgataaa taaaagtaaa
     7681 ccgtatcttg agcagatagt caactgcaaa cagaaaaagg actgtgacaa aagtcgtgtc
     7741 aaagtccctt tctacaaaca aacggtgttc cagaagtagc ttcttcaaaa aataagccgg
     7801 aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     7861 ccacttctgt cacaacctca atatggtcta ttattggttt tacctgttaa atcgaggatt
     7921 cttttccgaa gtaacgttgc catctcttac gcatacttgc tgacataggc tgagcggctt
     7981 ctttgactgc gcagtatttg tctacgaatg ttacgatata tccttctttg tacttaggcg
     8041 gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc tcgctcaaga tccgacaaat
     8101 cggtgatctt ttctgcattc tttacagcga ttcttggatg gatatacgca tgtgtgcctt
     8161 catcaaattt cgtcgtacgc caatcatagt agaacagatc atgcaaaagt ccagcacgcg
     8221 ctgttgcttt agcattgcca ccccattttt ttgcgagcag ataactatag taagaaacac
     8281 tgatggaatg ctccaaacga gtggaatgaa catgctgtgt gtaattcgct aattttttca
     8341 ctgcttctgt ttctaaaaga tcttctacat aagacatata ttcttcgtct tcacgccatt
     8401 tttctgtcat aattatcgat ctgccacctt tttgaatttc attttcttct gaaattgatc
     8461 taagtatacc accagtgttc ggattattct agtcgccttg gataaaatag ccaaagctta
     8521 atagttcctt aatcataaag aaaaatgata aagaacaatc cctgccgcaa ccccaacatt
     8581 caacgattcc gcatttcctt tcatcgggat atatacgttc tgatcagtca aagacaagat
     8641 ttcctgactt accccctgac cttcgttccc catgattatt gcgaagttat ctgatttttc
     8701 tactttgtct aaggcgtgtg cttcttcatt taattctgtg ccatacactg taaacccttt
     8761 gtctttcatt aaaggaatca cttctgtcaa atctttttgc aacacaggca aatgataatt
     8821 gcttccctgc atgctgcgca gcacctttgt actataaata tctgccgttc ctttgcctaa
     8881 taaaacacca gccatatcaa atgcatctgc cgtacggatc atcgtcccta catttccagg
     8941 atcttgtaca ttatctaata acagccatcc tcctgaaaaa tctggcgtgc cttcagccgt
     9001 taacgccatc actgctaaga ttccttgtgg tgtaggtaga tccgatacag catccatgac
     9061 ctcatccgaa actagaaaag tagaaatctc tttgtttgcg gccagccagt ctccccattc
     9121 tttcatgcct cgctgtgtca caaaaagttc ttttatttct gcatcagacg acgcagcttc
     9181 ttcaatcaga tggaaccctt ccaaaagata ttcttccttt tcttcccgat atttcttttt
     9241 atgtagtttt tttaattctt tgattcttgg attttttgct gattggatct ctttcacatt
     9301 cctcacctcc ccatgattat atcatggaat aggtgcttga gcttgtttca tcctaaaaaa
     9361 atcggtatca ttaatttata aaataggaaa gaaaggtgac gaacatgcgc aaagttaaaa
     9421 tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta catgaccaag atggtcgctg
     9481 atcaactagg cgtgactgga acggtaaaaa acgaagatga cggctcagtc acgatcgaag
     9541 caatcggcaa tgacgacatc atccagaaat ttattgaaga agtcaagaaa tctccgagcc
     9601 caagtggacg agtgcagtat gtggatgtcc aagaagaccc gatgattgag gaacgaaaga
     9661 aatttgatgt ggtgggataa ggttgtgaaa aagctggtct gcatcaagca ttaaggacag
     9721 gttgtgaaaa atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag
     9781 cttttgaaca ccgtcaataa ggttgtgaga aagtcgctta gttttgagta gtaaggagaa
     9841 ttttgtgaaa atggctttcc acatttttgc ataaaatttg acttaatacc gagaaactga
     9901 cttttgaaca ccgtcgataa ggttgttttt gagacgcttt gcctcaagta ttaaagatag
     9961 gtagtaaaaa agctgatcta taacttgcaa taaatttgct taacccatat tttccagaca
    10021 gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca cttggaatca ctcttttctt
    10081 tccaagtatt tctttcttga ttttttgtct ttttttcaaa atttttcgct acgtaaaaga
    10141 acgaatattt atatgaaatt tctatgaagt tgattgaatt ccatgcgctt ttttagtata
    10201 gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg gaaaaactgg
    10261 ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt gcggatggat
    10321 tcaaacggaa accctgacac ttcgggaatc gtttatcgag tactcgtcca tcctatgggc
    10381 caagcgatta catatctagt tgagaatttc aattggtctt atggctgggc agttattgca
    10441 atgactgtga tcgtccgtat catcatcttg ccactaggta ttagtcaatc gaaaaaaaca
    10501 atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc ccaacaaaaa
    10561 ttgaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat gcagcaagta
    10621 taccgcgaaa acggattgag tatgaccggc ggaatcggct gtctgcctct attgatccaa
    10681 atgccgatct tctctgcctt gtactttacg gcacgttata cagaaggtat tcgggaatca
    10741 tctttctacg gcatcgattt aggctcacca agtatggtac ttgttgtcat tgcaggggtc
    10801 gcctatctgc ttcaaggata catctcaacg atcggtattc ctgaagaaca gaaaaagacg
    10861 atgcgtacga tgctaatcgt ctctcctgct atgatcgtct tcatgtcgat cagtgcgcca
    10921 gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca aacattcatc
    10981 acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg cagaagagtt gaaacaaaac
    11041 ccgccaaaac aagtcgtcac tccaagaaaa gacgtgacag aatcagcaaa gccagctaac
    11101 aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca gtacaaacaa caaaggccgc
    11161 aacgcaggaa agcagcagaa gcggaaataa aaacatagga caaaaaagat gagagctaag
    11221 ccctcatctt ttttgttact cttcctcttc tgctattcca ttaattaatt cgttgacaga
    11281 gacaatgctt tcacatcctg cacgaacaaa ctcgggctct gaaccaatca tttgtgagtt
    11341 gataaattca atgaccatcg gaagattcat cccagcataa agagtaatct gttgtccttc
    11401 cattattagt ttagaaatga tatttgcagg tgtaccacct aaaagatcgc atagtaccac
    11461 aaaatcattt aacgaatctg tgacagtgag aaacttttgt ttgtatgttt cagtgccatc
    11521 ttctggtaat aaaggtaacg tgtagatatc ctcttgaggc cccatgatca tttctgtact
    11581 ttttttcaat tcctcacaaa attttccgtg actaatcaat accaattgtc tactcatttt
    11641 tcgctctccc ttatttcgaa atcacaccca atgcagataa agcaacacac acaattaata
    11701 cgataaaaat cgctcgagta ggagtcatgt ttttctttcc taataaccaa taaacaccac
    11761 cgacgatccc agcaggtaaa attttaggta agatcatatc gatatagttt tgcatattca
    11821 atgtcacatc gccaatcgtt ggtgcccatg aaaatttaac attgatcatt gtcgcaacta
    11881 atgcccctac catgaatacc cctaataaag tggctgcgtc cgttaatgca gttaagcgag
    11941 attgcatagt agtaactaag gaaattcctt ctttgtatgc aaattcaagt tgcttccaac
    12001 gaaaaaccat gacaacgatc tgagcaaaaa tccatataaa cacacctatc ggatttccat
    12061 cagttgccat attcgccgcc aaagccccaa aaatcgttgg aatcaaagca gcaaatacgg
    12121 aatctccaat cgctgcaaag gaacccatca atccagcttt cagccccgat acggtttcct
    12181 ttgaagcaat tccctctttt tcttccattg caagatcaat cccagtaatg attgtattaa
    12241 aaaaattaga agtattaaaa aattgcgtat gtgttttcat catctctttt aattctggtg
    12301 tattgtctcc atatattttt cttagttgtg gcaagatagt atataaatag ccagaaccct
    12361 gcattctctc atagttccaa cctaattgaa aagtgaacaa acttcttcga ttgatttgct
    12421 taaaatcttc ttttgttaat ttgtaattag atttcgtcat cttcgatttc cccctcaaca
    12481 gaatgtgatg gctcactagc taatggttga tggtctttgt tttgatcatt cttataactg
    12541 atcattgcta gtgctagacc aatcaatgct acagcaagca tcggcagcga attgaaagtt
    12601 gccggaagat ctttaacaac gcttgccaca cttgttccta ataattgaac attagagaat
    12661 aacgttgcta ataatgccgt gatcgtaaat cctaaaataa gataagatag atgttttttt
    12721 actggtaaat agcgcagaag aatggcaaac ccaacagctg gtaaaacagc tcctgctact
    12781 gaaagtccat cgcctagcca ttttagattc ccgtttaaaa cagaaacaac attttcaact
    12841 aatccaccac caaatgctaa agctaaaaat acaggaatca tacgagagag cgaccaaggt
    12901 aaagctccca ttaagaaatt gcgttcgatt gcttgataat tcatgtcttc tacgtgacgg
    12961 tcaatacgat gggcaaagta tgtgttagcg aatctagcta aaatatccaa ttggatcatt
    13021 aagcttgcta caggcactgc aatagcagca atcgcttgtt ctggattcat tccaagagaa
    13081 atcgagaagg cagttgctaa aatcgtccca gagttggcat ctatttttga tgcgcctcca
    13141 aacgtcccca ctcctaaaac tgtcaactgc atactgccac caataatcaa acctgcgttt
    13201 aaatcaccca ttactaaacc tgcatataac cctgcaaaca ctggcgcact cattgaagaa
    13261 tagatctgta attcatctaa aatttgataa cctgcatata atgttaataa taagatctgc
    13321 caccattgaa tatccatgga tcattcctcc tatttgatta aaagattcat aaagtcttct
    13381 gctttatcgt ttggtaccat ttgagcaatt aaatgtatac caagctgatc taatttttta
    13441 aatacttcga tatctgcatc taccacattg attgattttg taatggaccg tgtttcattc
    13501 gtttgtgaca tattccccac atttacttct ttgataggaa ctcctttttc tactaaagat
    13561 aagattcgat ctggcttttt tgctacgatc aatactcgtt gagagtcata tttgccagct
    13621 aaaatatttg ttgctgcttt ttcgattggc aaaatgctta attttacccc tgctggagtc
    13681 gctaacttca atccactttt ttcaattgtg tttgtggcta cctcatcatc cacgaccatg
    13741 attcgactaa tattcagttt cgttgtccaa agatttgcta cttatccatg tatcaaacgt
    13801 ccatcgattc ttacaccaat aattgccatc atgcattctc cttatctatt ttttctattt
    13861 attgttcaat taaaactgtg acatcatcaa atatcccttc tgtttcaaag ataacgtttt
    13921 cattttttct ttcttatatt tcatttttaa gctaagaaca aattccttcc caactaaaga
    13981 aaacgctttc ttttatattt aagttcattt gattttctta acttgaatgt gtttcatcaa
    14041 catcgtaact ttcatagggt cgatccagcc agttttctct tgttgcgcgt ttgccatacc
    14101 acaagccata gaaaatgcga gaatctcttc gattgattgc ctttttgata acgcatgggc
    14161 cgatcctgca agggtcgcgt cacctgaacc aacaggatta acaactgata ttttaggaat
    14221 aatgacttcg taaaaacact gagcaacttt tgccacagca cctctctctc ctaaagaaac
    14281 aacaacccat ggcactcctt caaatattgg atgagctaat aaggactgaa ttttttctaa
    14341 ttgaatcgaa gaacagatac gttggccaat cagttctcct aattcttcca gattgggctt
    14401 gataagtagt ggcttctcct taccttccaa tacttcaagc aaaaatttac cagaagtatc
    14461 tagtaatatg tctttccctt ttttatcagc taaagcaacc aattgcttat agtaatcaag
    14521 agggcatcct tggggtaaac tacctgagat cgtcataata tctgatattt cgaggagctg
    14581 ctcgaaatga tcggtaaatg tctgttgttc tttctttgtt atgaaggggc ctcgttccaa
    14641 aagctctgtt tgtctcgaac tatgcaggat agcaatagaa tcccttgtct ctcctttaat
    14701 tggaacaaac tgatgcatga tccctaactg atctagctgg ttctttagat attcaccgtg
    14761 ctggccacct attaaaccag tagctatcac ctcttctcct aataaattta gaaccctcgc
    14821 cacattcact ccttttcctc ccgctgtttt attgactgtt tcaactcgat tgactttgtc
    14881 gaggtttaat tcttcaattg tatacgcgat atcgatggaa ggattcaagg taactgtcgt
    14941 aatcatttca tcttcacctt tttgcttttt gttttattga accaaggggt agcggtttca
    15001 tttattactt tatttaaagt ctcgatgttt tctctcccat ttgttcttaa ccaatgaata
    15061 gctccgtctt ttccattttc gacgtatgct ttgaccccat ctttccatgt cgcacgtcca
    15121 cacaatacac cattgaaggt ggaacccgat tcatgagcaa aaatcagtgt ttgttgaaat
    15181 aattctgttg aaacaccccc actcaaaaaa atgaatggta atggagtcgc ttcactttgc
    15241 tccttaaaga aggcagcagc ctcttctttg ctgtgaataa attcacctgt ggaatagcca
    15301 ttcacataat tcatattgac tggtacttct actttcaaca catcaacttt atattttgga
    15361 tcagaaaatt ctttcatcat ctcaataact ttgtggggct tgactttcgc atactctgga
    15421 gaagaactat cgcttatttg tgcatcataa gaaactaact ccaaatagaa gggacgattt
    15481 tctccttcgc actcactacc taaacgttcg atgaacacat gcttcaaatg attgatttcg
    15541 ggtgcttcat caacatcgta gtaaagtaag aattttactg catccgcttg ctgttctttc
    15601 aatctgagag aagaccaatc cgctaataaa tcaggtagac gaccaggcgt agttgcgtca
    15661 tagccggttt tctcataagc taacaataac cccgtctctt ttgctctgac ttttgctgct
    15721 ggcaatccat attccggatc caataaaata gcagaagcat agggtgtcag ctcagctgat
    15781 actagttctt tgaattcact gatttggtct tcacttggct cttttcccaa cgatttgatc
    15841 atttttttta gagcaccacg ctgatcgatc gctaatgcac taataatccc atcttttgtt
    15901 gatagtctct ccattgccct atttttttct tcattcatct gtcatttctc cttcggtttc
    15961 atacttatga ataatgacgc ccttgactac tcgattgacc gtgcctgttg gagatggtgt
    16021 atctggttta ttagctactt tgattgaagt atagagtgca atgatttgtg cagtcataat
    16081 catcgctaat gcaagatagg catcgggcaa tttactcacc gagtcttcca atagcacagt
    16141 catacctgag aaatcaatag tacccggttg cgcaatcgca atgacattgg cagtgacccg
    16201 atcttctttt atttcctcta aaatatctaa gtcataattt cgcgtatatg gttgattgct
    16261 cacaaaacct acaacgagtg tctcgctatt taaaaaagac tttggtccat gacgaaaccc
    16321 catcgaagaa tcaaaaattg tagcaacttt acctgctgtt aattctaaaa tttttaattg
    16381 tgcttctctt gtcgcacctg ccaaacttcc tgaacccaga taagcgatcc gttcaaaaaa
    16441 atcaggtaat aacttttgaa tttcttgatc tcttgaaaga agatcttgac cgattttaac
    16501 taattgttca agatatcttt cttttttctc cattgctgtt tgatcaaaga tccataaagc
    16561 actcaataac atacacgtaa aacttcctgt catagcaaaa cctgcatcgt ttgctctctc
    16621 tggcatcaat aacagataat tctgtttatc atcctgtgcg ttttttgcca acatcccctc
    16681 tttagcacaa gtaatcgtca aatgaaacaa tgtttcaact tgttcatttg caatttgcac
    16741 agccgctaca ctttctggac tgttgccact gcgagcaaat gaaactaata aagtcggttc
    16801 atttggaaac aaaaattctt ttggtgaagc tacaatatct gtcgtgccaa aactgtggaa
    16861 gataaaaaac tgattgtctc catgttcatt tagatagggt aagagcgtat ccccaatata
    16921 ttgagacgtt cctgcacccg taaaaataac acgtattctt cttccgtttg cttctttttt
    16981 tatttgatca aaaaatagag ctaaattttc ttggctttgc atcaatctat cgattgtttc
    17041 tttccagaga tctggttgtt gttttatttc ttgtgtcgtg attgctgcac ctatttgttt
    17101 taatttgttc ttcgatagct caaacatgtt gttctccctc tcaatcattt tcggatatgc
    17161 cgaattttat aatgaaactg gtccgctctt gctacactat ctgtatattc gataatctca
    17221 tttttcacgt tataggttat cctcgtcaga tgtaagacgg gagcgccatc agcgattcgt
    17281 agcacaacag catctttttt ttgcacgata cttgcataaa actcttcatc agctaattga
    17341 acttgttgtt cataatcctg tgaaaacaca tcatacaacg gtttactttc aagtaacggc
    17401 aaatcaagtc ccataaatag tttcgccggt agataagaac gctcaaccat caatggctct
    17461 tcatctgcca aacgcaatct tttaagttta aatacagttt cacctaaatg gatgttcaaa
    17521 ttcttagcaa ttctcttatt agcttccatt tgtctaaatt ctaaaatgac cgtcttaggg
    17581 acgcgcccta tttcgcgcat ttcttctgta aagctatatg ctccagctaa gttcgatgac
    17641 ttatcagaca aatcagagac ataggtacct tttccatgtc ttcggtaaat ataaccttga
    17701 ttttccaatt cctgtaaagc cagacgtacg gtggtacgac tcaccccata gtgttgacaa
    17761 agttctcttt cagataacag cttatcatgc ggagtcatct gtgtttcgat acgatccttc
    17821 aataaatcaa ctagttgata atacaatgat tttcctttat ttgagctctc cataaaaatc
    17881 acctcttctt tactggttat aaccacaaca caagaatagt gaattattaa tcatttgtca
    17941 aaggtttttt agaccaaaaa agctattata acagctcttt aaagctttat atcttgaata
    18001 gacaaagata cctcaaaaat accagttacc aataaaagaa cagaaaaaaa gacgaaccac
    18061 ataagcgatc cgtcaaaatg gtctaactta tcgagcagcg tgatccacat taatccctat
    18121 gatattccta caactatcgt tttattactt ccaatatcat ttacaacaac taaaaatagc
    18181 ttattaatga caaatgttat tattagtttt agataacttt tgcagtttta tacttttttt
    18241 ctataaaatt tgacaaacat ttttttaaat aactaattat tttttgaatc tgtcactcag
    18301 acaatccttt ctcttacttg ttaacataaa tagaagttgc tgtttcttct aatagtgtac
    18361 ttaattcttt aatattttgt tttccttctt ctaacatcca actactcgct ttttcttcgc
    18421 cttcggtaac aaaaggtgct actcccttcg cccatgttgc tcgcccacat aagacaccat
    18481 tgaatgtcga acctgattgt tttgcaaaac gcaatgtatc ttgaaatagt gcagcgctga
    18541 ctcccgcact taaaaaaata aacggttgtt cagtcacttt cgattgttca tagaaatacg
    18601 cggatgcctc tgcttgacta taacaagtat cttgcccata tccttccaca aatctcatat
    18661 tcactggtac ttctactttc aatacatcca cttgataacg ttcttttgaa aattctcgca
    18721 tggcttcaat cactttatgc ggttttactt ttgcatagtc aagagaagac ggatcgccaa
    18781 tagttgcatc gtaagaaagg agctctaaaa agaaaggtat gccttctgct tcgcattctg
    18841 aaccaatccg ttctataact atttgttttt gcacattaat ctctactggt tcatcaacgt
    18901 catagtacaa taaaaattta caagcatccg cacccacttc ttttaacctt ttcactgacc
    18961 aactattcag taaatctggt agtcggcctg gcattttcgc atcatagccg gttttttcat
    19021 aagctagcaa caaaccacat tctttctctc gaagcgctgc tgcaggcaga ccatattctg
    19081 gatcgagtaa aatagcggaa gcatacggag tcaggtactg agaaaccatc tgtttaaatt
    19141 ggatgatatc ttctgatgta gcttctttat attggttgat cattttcttc aaagcaccac
    19201 gttggtcgat agctagcgcg ctaataattc cagcttcatc cgtcaatcgc ttcaaacgtt
    19261 cctgtttttc tatactcatt ttcttcatat atcttcacct atttctttag ttaatattgt
    19321 agctatttct atttttgatt ttgcagctct caactgttct gtaacctctt ttcgcatcaa
    19381 tgatcgtgca acgagagaca ataatttcaa atgagtcgtt cctgcttctg aatctgggat
    19441 aaataacgca aaaataaaat caactgactg tctatctaaa ctgttccatt caattccttt
    19501 tttcagtgtg acgataatca aactaggttc attgattgtt tgagacttcg catgagggat
    19561 agcaaaacct gacattattc ctgtagtacc ttctttttct ctttccagaa gtttttgata
    19621 tacttctttt tcgtcatgag cgattcctaa acagaatgtt tgctttgcta aaaacttaaa
    19681 tgcttcttct tgattatcta atggctgttg taaaaaaatg ttctcttcat gaatgattgt
    19741 catgataaac ctcctgctct tccttcttcg ataactgtac gatttgttgc tttattatcc
    19801 ctcttcttgt ctttatcagt tactcttttc ttttggatca aacctagtat cactccacca
    19861 acgatcgaac caataagaat cgctgtaacc cattggattt ttcccgtcac tacaggtaac
    19921 actaaaaaac cgccatgtgg ggctggtact tggacaccaa acagataggt caagacagca
    19981 gcaattgatg agcctaacat catcgttggt aatactcgga tcgggtcttt cgcagcaaac
    20041 ggaatagctc cttccgtaat atgagttgat cctaaaataa aattaaccaa acctgcatta
    20101 cgatcattag tatcaaaata tttaccaaac aataaagtag caaaaccagt aatcaacggt
    20161 ggtgcaatgc aagctgctga cgcaccagcc ataaaagtcg tgtttccttg tgtcaaaaga
    20221 gctgttcctg ttacatatgc cgccttatta accggtcctc ccatatcaaa cgcacacata
    20281 cagccaacaa tcacgccaag aacaagtgga ttcgaatatt caaatccagc taaaaagctc
    20341 atcattcctt catttatagc cttcattgga cctgccagca aagacatcac gatgccaata
    20401 aatgctacac caataactgg atataagacg attgccttca agccatctag ttgtttaggt
    20461 aaaattatca gtagcttttg caaaaataag ataaaataac ctgctaagaa cccagctaaa
    20521 atacctccta aaaatccagt accgccattc atggctacga ttccaccaac aaatccaacg
    20581 actaaccctg gacgtttcgc gatcccttct gcaatataag ctgctaaaac tggaaccatc
    20641 attcccattg ctgcatcgtc aactgctttt accattgccg ctgtagcatt gtattgtgca
    20701 tgatctggat cagaagaata gattccccaa acggcaaatg aaatagcaat caatactccg
    20761 ccagctacca cgaaaggcaa catatgagag acaccgttca tcaaattttt atagatgcta
    20821 cgaccaaaat tagcaccact tatcgcttct ggttctaaag tatcagcagc ttcattatct
    20881 gctaagacag taccttttcc tgccaatgct tcgtcaatca gccgttgtgc atccttgatt
    20941 cctgtactga cagaaacatc aatgactcgt tttcccacaa atctttctga atggacatct
    21001 ttgtctgcgg caataatgac agcatctgcc tgtttgattt cttcagccgt taattcattc
    21061 tcgatcccaa tttgtccatg ggtctccact ttgatcgtga tgcctttttt cctagctgct
    21121 tgctctaatg cctcttgcgc catgtaggtg tgcgcaattc ctgtcggaca accggttgct
    21181 gcaattagtt gatattttcc catctttcga acctctcttc cttgattttg atttgtttac
    21241 ttaattcctc cacatccaaa aaatcagtca gtcctttacg aaaagcagta gagctgcctg
    21301 ctgctatgct tttacgtaac gtttcatcaa gcgatcgtcg attcatgtat cctgctaaaa
    21361 aagtgcctaa catggcatca cctgcacatg ctgtattgac aacttttcct tttggcgagt
    21421 ttcctcgaat caccctgtct ttcgtaaaaa gaatcgctcc tttctcaccg agggataaca
    21481 aaacattttc tgcgcctgac tctactagtc gttgtccata aacaaagtaa tcttcatctg
    21541 taaccatttt tcgtccgaac caagaagaaa gttcttcctc atttggtttc agcaaaaacg
    21601 gtcgataggg taaacaatcc atgacttctt gatcactact atcaataatc aaacaaatcc
    21661 gactgatttc tattaaaacc tttggagaca gccctcttgg taaactccca gaaacacaaa
    21721 gataatcccc tgcttgtaat gctcttattt gatttaaaaa attatgaaca gctgtctgcg
    21781 gtatttcagg tccttgattc actaatttgt attcttctcc cgtttgattg acttgtgtaa
    21841 atacattgat acgcgtcata ccaggcactt caataaactc tgtttggatt tgtttctgct
    21901 gtaaatagtc ttctatatac tttcctgtga acccagcaga aaaacctaat gccgtacttg
    21961 ggaaatctag catctttaat actagtgaca cattcacccc ttttccgttt gcctgaatgt
    22021 catcctcttt tgtgcggttt accataaaag gatgcatctc atctgtttca ataaatagat
    22081 caattgccaa attcatcgtg catgtgtaga tagacataac caacgacctc cttgattcta
    22141 ttatgaaata cgcgaaatag aaaatgtttt cttaatcgtc ccaacacaaa gaaaaccttt
    22201 acatttataa actcgtcgtg ctttttttaa ctaacttttg gtgaaagaag gaaagaatat
    22261 gaatcacaaa ttaagtgaaa cagagcagta cctctggaac tttatcgaac acctcatcct
    22321 agagatccct aactattcca tcgtgaaact aagtgaacaa gcaaatgtct caacagccac
    22381 cattgtgcgg acaatgaaaa agaaaggcta tgaagggttt acttcgttta agcatcatct
    22441 aaaagaagga gaaaacaaga acatcaattt ttcattttta gataaagtag acaaaggaat
    22501 ccgccgagcc attctaaaaa atgaacaaga agtggtacgt acaatcaata tgttggaaat
    22561 cgggaacatt gaagatgcaa tacaaagaat caaatttgct gaccgggttt ttgtctttgc
    22621 ccgaggattt tcagaaatga ttggccaaga aatgctggta aaacttcaat taacgggaaa
    22681 aaattgtcag atgcatactg atccagaaat tatcaaaagc atcagtcgga aactaactaa
    22741 gaaagacagc gttatttttg tttctttgaa cggtgagaaa acaaataaaa acatcaaaaa
    22801 taagatttat gtctttattt ttcaattttt taaat
//
LOCUS       DACDSR010000038        22832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDSR010000038 DACDSR010000000
VERSION     DACDSR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.038"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            37..591
                     /locus_tag="IWK15_001496"
     CDS             37..591
                     /locus_tag="IWK15_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9547093.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(764..1237)
                     /locus_tag="IWK15_001497"
     CDS             complement(764..1237)
                     /locus_tag="IWK15_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9547094.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1249..1785
                     /locus_tag="IWK15_001498"
     CDS             1249..1785
                     /locus_tag="IWK15_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9547095.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1825..2196
                     /locus_tag="IWK15_001499"
     CDS             1825..2196
                     /locus_tag="IWK15_001499"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9547096.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2480..3751
                     /locus_tag="IWK15_001500"
     CDS             2480..3751
                     /locus_tag="IWK15_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9547097.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3996..5021
                     /locus_tag="IWK15_001501"
     CDS             3996..5021
                     /locus_tag="IWK15_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9547098.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5315..6424
                     /locus_tag="IWK15_001502"
     CDS             5315..6424
                     /locus_tag="IWK15_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9547099.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6507..7403
                     /locus_tag="IWK15_001503"
                     /pseudo
     CDS             6507..7403
                     /locus_tag="IWK15_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7419..8243
                     /locus_tag="IWK15_001504"
     CDS             7419..8243
                     /locus_tag="IWK15_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9547100.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8275..9585
                     /locus_tag="IWK15_001505"
     CDS             8275..9585
                     /locus_tag="IWK15_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9547101.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9733..10992
                     /locus_tag="IWK15_001506"
     CDS             9733..10992
                     /locus_tag="IWK15_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9547102.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11002..11874
                     /locus_tag="IWK15_001507"
     CDS             11002..11874
                     /locus_tag="IWK15_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9547103.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11886..12716
                     /locus_tag="IWK15_001508"
     CDS             11886..12716
                     /locus_tag="IWK15_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9547104.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12756..14939
                     /locus_tag="IWK15_001509"
     CDS             12756..14939
                     /locus_tag="IWK15_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9547105.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15000..16220
                     /locus_tag="IWK15_001510"
     CDS             15000..16220
                     /locus_tag="IWK15_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9547106.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16217..17677
                     /gene="gtfA"
                     /locus_tag="IWK15_001511"
     CDS             16217..17677
                     /gene="gtfA"
                     /locus_tag="IWK15_001511"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9547107.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17754..18860
                     /locus_tag="IWK15_001512"
                     /pseudo
     CDS             17754..18860
                     /locus_tag="IWK15_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19182..19391
                     /locus_tag="IWK15_001513"
     CDS             19182..19391
                     /locus_tag="IWK15_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9547108.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19384..19533
                     /locus_tag="IWK15_001514"
     CDS             19384..19533
                     /locus_tag="IWK15_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9547109.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19568..20352
                     /locus_tag="IWK15_001515"
                     /pseudo
     CDS             19568..20352
                     /locus_tag="IWK15_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(20636..21721)
                     /gene="xerS"
                     /locus_tag="IWK15_001516"
     CDS             complement(20636..21721)
                     /gene="xerS"
                     /locus_tag="IWK15_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9547110.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22055..22288
                     /locus_tag="IWK15_001517"
     CDS             22055..22288
                     /locus_tag="IWK15_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9547111.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22285..22692
                     /locus_tag="IWK15_001518"
     CDS             22285..22692
                     /locus_tag="IWK15_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9547112.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7801 a         3423 c         4382 g         7226 t
ORIGIN      
        1 cattaactca gaatattttt aagaagggaa acaatagtga gctataatgt acaaccgtta
       61 aggacaaatg aagagataca agattttcag ttttggctaa gaagaacaag taatccggaa
      121 agagatagtt ttttattttt gtttggaatc aataatgggt tacgtatgtc agatattata
      181 ggattaaaag ttggagatat acgaggcaaa agtaagccta ttattgttga acataaaaca
      241 ggtaagcgaa aaccgatttt tatagataat ttgagagaag aaattttatt atatactgaa
      301 ggtaaagaag aaaatgattg gttatttccg agtcggcaac aaggtcgaca tataacaagg
      361 gatcgtgtct atcagatata tgcagatata gcagaaaaat taggacgaga tgatatagga
      421 acacatacat taagaaaaac ttttgggtat cactactaca agaaaactag agacattgct
      481 accttaatgt ttatttttaa tcatagctcg caagcaatta caaaaaggta cattggtata
      541 actgaagatg aaataggagc atcacttcgt ggatttaaac taggtgtata atttaaaaat
      601 ttttataaac agtaaagaca aagattaaaa gctattttta tctagaacga ttttatttca
      661 taaagttcgc ggaataaaag ctggggttac tggtacttgg tataggttcg tgttatataa
      721 actaagtaat taaataacat ttttaggttg gaaaaggatg aaattaaaaa aagagaatca
      781 atacggtctc aaaaacgcaa acgctgcgga ataagaaaaa tactgataac tcaacgttta
      841 gcagactatc taatagggat agtctgcttt tttcgttttt gaccatactt ttgaccttct
      901 gcaaacttcc ttgcttttcc tctgtagaac tcatgaaatc tgcaaaacga attgccgttt
      961 cctctgtctg ctttttcgcg atatgggtgt atatatcatc catagtggtt ttaatatctg
     1021 tatgtccaag tcgaacatac acttctttga cagacgcacc agattcaaag agcagactac
     1081 aatgggtatg acgaaaacca tgaagtgtga tatgttttaa gttattctct ttaatgatga
     1141 ttttcaaatt gtgatttaca tagtctaaat aaaggggctt attttcttct gatgaaaaca
     1201 aaagttgttc accatctttt ataggtttga ttctcatttt tagatattat gcccgcacgt
     1261 attcatcaca tttctattgt gaatcgcttt attcgaccga catttgactt ttatcataat
     1321 atactcggtt taaaattact aatgaaaaca attaatcaag acgatcatac catgtatcac
     1381 ctgttttttt cagataatca ccaccgtgtg ggaactgaac tgactttttt tgaagtacaa
     1441 gaagggaaca accagttttt tggaaccaat acgattgaac gaacgatact aaaagttcct
     1501 tccgaagcat cattgcattt ttgggaaata tattttgaaa cacaaggcgt ttgccattat
     1561 ggaatcgaat cattttctgg acgacctatc ttacgttttg aagggccaga tgcaacacga
     1621 ttagctttag ttcccttacg cgaatttgaa gatattgatg attatttccc gtatgtcaca
     1681 gaccagatcc ctacagagca tgccatttta ggaattgatg ccattcaact acgcgtacaa
     1741 tattccgatg cgactgaacg agagttattg tcggtaggct ggtagcacaa agaatacgtc
     1801 agtttctttt cgggtcatca acgagtgaca atcttagaaa atcaagacac tcagttctat
     1861 caagaagttc agattattca agatcgtgag aatccagttg ccattgaagg gattggtagc
     1921 gtccatcatg ttgcgtttgg cgtagaaaat aaatctgatt tacaacgcat cgacaaacaa
     1981 ttacaagagc gcaattttat aaattcagga ataaaagatc gtgaattttt tgtatcactt
     2041 tattatcgtg acccaaatca attattgatt gaaattgcta caggggaagg aaacctagat
     2101 gcaaaagcgt atgaaaatca aagtccacgc tttgaagaaa ttcctttatt tttaccgcag
     2161 tacttaaatt tcattcgtga acaagtggag caataattag ctaatcaaat ccatgacgaa
     2221 gagtaagagg ttgactcata agtcaaaaaa taaagaaaaa cgcatcaaat ccattctaaa
     2281 gaatgttgat ttgatgcggt tcctattttt ataaactatc attttcgact aataaaacta
     2341 cttatgagtc agcctctctt tttttcttga tttttatacc tctttctagg taaactaaac
     2401 atctgccaaa aataccgtgt ctcattgaaa cattctcaac tatttcaaaa taaaggggag
     2461 taccaaatga acgagtatat tgaaattatc cacgcaagag aaacaactta aaaatgtcag
     2521 tctcaaaatt cccaaaaata aaattagtat ttttaccagc gtatctggtt caggaaaatc
     2581 atcgattgtt tttgatacta ttacccaaga agctggtcgt caactgaatg acacctatga
     2641 tagttttacc cgtttatttt tacctaaata cagtcgtcca gaggttgatg agattgataa
     2701 tctttctacg gcgattgtgg tcaatcaaaa gaagattggt ggcagtgcgc gctcaacgtt
     2761 agggacgatt actaacatca atgcgttatt tcgtgtgttg ttttcatgtt ttgcaacacc
     2821 tagtttagga tatgcaaatg cttattcatt taatgatcct agtggaatgt gttctgtctg
     2881 tcagggaatc ggtaagacga ttgcattttt aataggattg ctttattttt cggctattga
     2941 taatgctgat tttgtatttt ctgatccatt taccgcagaa gttttgtctg gaattgagga
     3001 acaggcaaag aatcatggtt attttgtatt ggtgcataat gttaattcag cagaagatgt
     3061 taaaactatt cagagaaatt ggcgttttga ggggttcatt gttgttggtg tacgtgtaaa
     3121 agaatttttg gaattaaata acgtttttga tggtcctgtt gtttatattg atacgcatct
     3181 ttcaaaagag acattaagag aacttgaaca acaaatgaat cgtttatttg taaatacaaa
     3241 tgattaccaa gctagtacat tggcagtgga acatttgatt cattatggtc atgagaaaat
     3301 agcgtttctt tcttttgact acattgtagg tgaaccaagt gttattcaag aacgacatca
     3361 agcttatatc gatacattaa agaaatatca tttaaccttt aaatcgcatt ggatatataa
     3421 caactctgaa tttgagcgaa tttatcaaga attagctgaa tttactgcaa ttgttgttac
     3481 tggagattat ttagcggcta aattagtaaa atttttaaaa aataagaata aacgagtgat
     3541 ggaacaatta tcgattattg gatttgatga tattactttt gcagaattaa tggatcctgc
     3601 gttgacaaca atacgactaa atccaggtaa caagggggcg attgcttttc accaactgat
     3661 tggactggcc aaacaagaag aaataaaaaa tcaagtacta ctattagagg gagaattgat
     3721 ttctcgagac tctgttcaca aaatcaaata aaaaagctct gtgaaataag tatagttaac
     3781 aataaaatat ccgaacgata gagagaatga actgtgatct agcagttcct cgtcgcaaat
     3841 tcactagaat tagcattatt tactctaaat tgttcggata ttttttcttg tttttaatta
     3901 tgattacagt gcctattttt aacgaaattt gcttattttc aaaatgtatg attatatgtt
     3961 agtaaacact ttcaaatatg aaaagaggag ctattatggt aggaattagg gatgttgcaa
     4021 aacgggcggg tgtgtccatt gcaacagtat cgcgcgtgct aaatgaggat ccgacacttt
     4081 cagtaaccga tgaaaccaaa cgaaagattg cagaatcggt taatttttat cattatgtca
     4141 aaaaaagtcc cgttgcaaaa aatttaacaa atattgcatt aataacgaca gtttctgaag
     4201 tagatgagtt ggaagatccc tattttcgaa cgattcgcag agggattcaa gtcgaagcag
     4261 aacaaagcaa agtcaatttg aagaaaatta ttcgtttgtc agaaacaccg ttggagcaag
     4321 acgatttgct aaaatgtcaa ggtgtgttga ttattgggca ggtgctatct tctgtgattg
     4381 aagaagtatc agcaattaat tcgaatgtcg tagtgattga tgaccctagt attgaagctg
     4441 aagtagatgc agtttatact gatttaacaa aagcaacgaa tcagcattta gatcgattgt
     4501 ataaaaaagg acaccgaaac attgcgttta tcggaggagg acgtgttcgt ttagatagcc
     4561 aaggaaataa acacgtgtca gatgatgatt atcgaaaaat agcctatgag acgtggatga
     4621 aacaaaaagg attagaaaaa tatagtcaaa tttatttaga aggctggacc acacttgacg
     4681 gaatgcaggc agcagattta cttttgaaaa aacatgctaa agataagttg ccgactgcta
     4741 tcatggtagg aaatgatcct attgctgtgg gggtttatcg ttcacttcaa aaaaatggtc
     4801 tacatattcc agaagatatt tcgattgtga gttttgataa tattgaagtt gctgaatttt
     4861 taacacctgc attaaatacg gtgaatgtga atacagaaga aattggaaaa ctagcagttc
     4921 gcatggttaa tgaacgaata aaagaagtta gaaatgtttc gatcaaagtg attgtttcta
     4981 ataatattat tgttagagaa agtgaaaaaa atatcgatta aaaaagtaaa catccaaaaa
     5041 agggtgttta cttttttgtt tactaatatt tttagtaaaa aatctcgaca aaacattaag
     5101 taaacagtta tggtgttgtt aacaaaaaga aaacgcattc aaaacaggag gaattaagat
     5161 gaaacatttc aaaaaaatag ctttaacagg gatagctgtt ttaactggga cattactcgc
     5221 agcatgtggt gggggagata gtaattctaa agacgggcaa gtaactttaa gctatgcgag
     5281 ttgagatact gaccaagctc caggactaag aaaaatgtta gatgaatttg aaaaacaaaa
     5341 tcctgatatc aaagtagaaa tggagacaac gccttgggat caatattggg ttaaattaga
     5401 agctgcaaca acgggaggta atatgccaga tgtagtaacc atgcactcta gcgaatccta
     5461 taaatatatg tcacagaatg ctttgatggg acttgatgat gttatcaaag aaaatgatat
     5521 tgacatggga aactttactg aaggaattgc tgatttctat acgcttgaag acgaactata
     5581 cgcaatccct aaagatgctt ctgtagtggg gctatggtac aacaaagaat tatttgataa
     5641 tgcaggtgtc gattatccag atgaaacatg gacatgggaa acactcagac aagctgcaat
     5701 tgatttaaca gatgaatcaa aaggcatcta tggttttgca tcacatattg gaacagagac
     5761 tggctactgg ccatttattt atcaaaatgg gggagaagta tttgccgatg acaatacaaa
     5821 atcaggtttg aataccccag aagcacaaga agctttagct ttttatcgag atattgcact
     5881 tgttgatcat gcttcaccaa caattacaga attacaagat cctgataagt taagtcgttt
     5941 tcaatcaggt agattagcaa tgatgattga aggaaactgg tttgcaccgg gatttttgaa
     6001 aaatgactat ctagttgaaa atgttgcttc tgccgtgtta cctaagggaa aagttaatgc
     6061 aacaattaca aatggacttg gttgggctgc cagtgcgagt acaaaatacc cagaagaagt
     6121 gaaaaaacta atggcatttt tagctagtga ggacgccaat aaaatccaag cggaaacggg
     6181 ggcatcaatc ccagcactaa aaggctttga agacgagtgg gctgcttctt cagatacaat
     6241 tgatttgagt gcttttggaa aaatgttgga gtatggagtc cctcgtccat acaataaaga
     6301 tggattacga gctgaacaag cagaagttca aattattaac cgtattctgt ctggtgaagt
     6361 aactgttgaa gatggaaccg ctcaaattga aaaagaagtc aatgcgatat tgagcggaaa
     6421 ttaaattagt taattataca atatacaggc gaaaggattc tatttcgtct gtatattaat
     6481 ggatgaatac caaaaagggg gatattatga aaaatcgaat ttttggaaaa tatcgtaaag
     6541 atcaagtgat atggggctat atttttattt ttccaacggc attaggatta attttattaa
     6601 atatatggcc agcaattcaa acagcaattt taagttttca aaaaacggta gggttcggtg
     6661 atactgaatg ggtcggatta agaaattacc aaaaaatgtt tgcagacggt gaagtttttc
     6721 agtctttatt aaacacatta atttatgcag tggtttcgat acctagtatt gttgtattat
     6781 ccttgttggt ggcagtttta atgaatagaa aagttaaagg attgtcgatt tatcggacaa
     6841 tttattttct accagtcgtt gctgcaccat ccgcagtcgc aatgatttgg cgctggatgt
     6901 tcaataatga ttatggactg attaataaca tgttggctaa aataggtctc gatggaccag
     6961 catggttaac ggatccatcg attgcgattt attctattat cattgtagga gtttggagtg
     7021 ctattggtta taacatggtg ttattactag ccggcttata agaaattcct aaagattatt
     7081 atgaagcagc ggaaattgat ggtgctagtc caatccagca gtttttcaac attacgttac
     7141 cattagttac accaacatta ttttttgtag ttgtaacaac agttatcaat gctttccaag
     7201 tatttgatgt tatttttatg atgattactc ctaatagtac cgccatgttt aaatcacaat
     7261 cattagccta tttattctat aaacattcat ttattttaaa tgacaaggga tatggttcgg
     7321 caatcgttat gttcctttta gtgattatat taattattac tgcaattcaa atgaagttgc
     7381 agaaaaagtg ggtaaactat taaggaggct atcaaattat gaaaaataaa tttttttcaa
     7441 atgcgccaat ccatatcatg cttattttag gtggtattgc aatggcatta ccttttattt
     7501 ggatgatttt aacttcaggg aaaacgttgg ttgaatctac ccaaattcca cctacaattt
     7561 tccctgagaa atttcagcta agtaactatc aacaagtact tgatttatta ccattcatgg
     7621 ctttttatat caatacattt ttaatggttg ttggacgagt gattggctcg gttttatttt
     7681 cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc agggaaaaat atccttttca
     7741 gcttagtact tattcaaatg atgattcccg gttccatttt tattactcca cagtatttat
     7801 tagcacaaaa attgggtatt ttaaatacaa ttccagcatt aatattacca ggggtagtta
     7861 gtgcgtttgg tacgttttta ttaagacaat ttttcttagg tttacctgat gaattggagg
     7921 aagcggccta tttagaaggt gccaatattt ggacgatctt tttcaaaatt atgttgccgc
     7981 ttgctaaatc aggattggtt gcacttggaa tattcacagc gttgtttgca tttaaagatt
     8041 taatgtggcc attgattgtc aatacttcaa ttgataagat gcctttagct ccgggattag
     8101 caaacttaca aggacaatat gccacgaact tcccacaatt aatggcaggt tcgttattag
     8161 caatttggcc aatagttgtg atattcatcg ttttccaaag aaaattcatt gaaggaattg
     8221 ctacaacagg tagtaaatta taaaactatt aattagtgat tggagagtaa gtacatgaca
     8281 aaaattacat ttattggtgc aggaagtact atttttgcaa aaaacgtatt aggcgatgcg
     8341 atgttaactc caagtctaca agatgcacat attgcattgt acgatattga tgaaaaacga
     8401 ctaaaagaat caaatttaat gcttaaaaat attaataaaa attcgaatga aaatcgtgct
     8461 gatattgttt cttattatga tcgcaaagaa gcgttaagag aggctgattt tgttatcaat
     8521 gctattcaag tgggtggtta taaaccttca acagtgattg attttgatat tcctaaaaaa
     8581 tatgggttgc gacaaactat tggtgacacc atggggattg gtgggatttt tagaacccta
     8641 cggacactac cagtaatgtt agactttgct aaagaaatgg aagaagtttg tccaaatgct
     8701 tggttattga actatacaaa tccgatggcg atgttaacca tgggtgtttt aaaagcgacg
     8761 agtattaaga ctgttggttt atgtcatagc gttcaagtat gcgtaccaga attgtttgag
     8821 catttaggaa tagccgatca atatgactta aatgactttc aatggaaaat tgcaggcatt
     8881 aatcatatgg cattcttgtt ggaaattaca aaaaatgggg aagatttcta tccaacaatt
     8941 cgtcagttag ctgcagaaaa accaaatcca catccagatt ctgtacgttt tgaattgttg
     9001 aaacgttttg gttactatat tacagaatca agtgaacaca atgcagaata ccatccatac
     9061 tttattaaat caaaataccc agaattgatt gacgaattag ggattccgtt ggatgaatac
     9121 ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca tgcgagagga cattgtaaat
     9181 aatggggagt taacacatac gagaagccgt gaatatgctt catatatcat ggattctatt
     9241 acgacaggaa ctccgtcagt gattgcagga aatgtcttaa ataaaggctt aatcacaaac
     9301 ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg ataaaaatgg cgtgcaacca
     9361 acctatgtcg gtgaattgcc aacacaatta gctgctttaa atcggacgaa tatcaatgtt
     9421 caagaactga cagtagaagc cgctttaaca ctagataaat caaaaattta tcaagcagcg
     9481 tatttagatc cacatctatc atctgaattg tctttaacag atatcaaaaa tttagtggat
     9541 gatttaatcg aagcacatgg ggactattta cctgaatata attaaaaaga agctgaaaga
     9601 gtaaggaatt ttcaattcct tactcttttc ttagttaaat ttagtaaaaa tattgacaaa
     9661 acacttagta aacagttaca ttagtgttaa caaaaagaaa acgcattcaa attgtataag
     9721 gaggagcata aaatgaagtg gtctaaaaaa attattttag cttgtatagc actaggagca
     9781 acgatctctt tgactgcttg tggaaaaaat caggaagatg caagcggtaa ggtagaaata
     9841 gagtttttta atcaaaaaaa agaaatgact caaacgattc aggagattgc taaagatttt
     9901 gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg taccgaatgc tggtgaagta
     9961 attaaaacac gtatgcttgc aggcgatgta ccagatgtca ttaatcttta tcctcaaagt
    10021 atagaattga aagaatgggc gaaagctggt tatttagagg acttaaccga agaaccgtat
    10081 ttagagaata tcaaaaatgg atatgcacaa cgttttgcta ttgaggatcg tgtatatagc
    10141 ataccattaa ccgctaatgt ctatggtttt tactataaca aaactgcttt tgaggaaatg
    10201 gggattcaag caccagaaac ttgggcagaa tttgaaaaaa tagtagctga gattaaagat
    10261 caaaacaaag taccgtttgc gattgcagga gcagaaggct ggactttaaa tggttatcac
    10321 caactagcta tagcgactgc tgctggtggt gaagaggaag cgaacaatgt ttggcgtttc
    10381 tcagaagtta atggaattaa tgctgaaagc aaagaaatgc aaaaagattt taatcgatta
    10441 gatttgttaa gagaaccaaa agctttgcaa aacaactggc aaggtgccgg ttataacgat
    10501 acagtagtta cttttacaaa aggtgatgcg ttgattatgc cgaatggttc atgggcaatg
    10561 ccgatgattc attcacaaaa tccagatttt gaagtaggaa cttttccttt tccagcagat
    10621 gaagcaggtc aaagtctaac gattggcgca ggagacctag ccttatcagt ttctgcaact
    10681 tctcaacata aagaagaagc caaacgtttt gtagaatata tgacaacacc tgaagcgatg
    10741 cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg agggcgttat cgagaatact
    10801 caggattcac cattaagtgg tttaactgaa cttgctttta ctgatcgtca tttagtttgg
    10861 ttagccaaag attggaatag tgaaaatgat ttttatacct taacaacaaa ttatttacac
    10921 aatggcaatc aaaaagccat gattaacgca ttaaatgctt tctttaatcc aatgaaagct
    10981 gacggaaggt aggaaaataa aatgaaaata aaagaaattt taaatcgtta ctgggcaatc
    11041 atttttgtag caattccaat tattttgcag atgatttttt tctactttcc aatggttcaa
    11101 ggtgcttttt acagtttaac taattggact ggtctgactt ataattttga ttttgtgggt
    11161 ttgaacaatt ataagattct tttttctgat ccgaagttta tcaaatcgat tggcttcact
    11221 ttaattgtga cgctttgcat gattgctgga caaattggtt ttggtatttt gattgcgcgg
    11281 gcgttgaacg caaatattaa agggaaaaca ttctttagag catggttctt ttttccagcg
    11341 gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa tttttaacta tggtttacca
    11401 aaaattggta gtatgttagg cattgaattt ttacaaacca gtcttttagg aacaacatgg
    11461 ggagcgattt ttgcagtaat ttttgtttta ctttggcaag gaattgccat gccagtcatc
    11521 attttcttag cgggttttca aagtattcca caagaaatta ttgaagctgc tagaatggat
    11581 ggggcgaatg ataagcaaat tttttggaat attgaaatca cctacttatt accaagtatt
    11641 tcaatggtat ttattatggc attaaaaggt ggcttaacag catttgatca aatttttgct
    11701 ttaactggtg gtggaccaag cgatgccaca acatctttgg ggttacttgt ttataattat
    11761 gcttttaaaa acaatcagtt tggttatgcg aatgctattg ctttagtctt atttatctta
    11821 attgggattg tttcactggc acaattaaaa ttatcgaaaa aatttgaaat ctaggaggtt
    11881 tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata cttgctttta ttattagggg
    11941 gcttcatgat tttagttccc ttagtttcaa cgctattcgg ttcatttaaa acaacacccg
    12001 atattatgaa taatttcttt cgttttccta atccagcgac tttggaaaat ttccgaagat
    12061 tgttagcgga tggaattggt cattactttt ggaattcggc aatcatcaca atcattgcag
    12121 tagttgctgt gacagcaatt attcctatgg ctgcttattc actagcaaga aaaatgactc
    12181 gtgggaaagc atacggattg atgtatttat ttttaatttt aggaattttt gttcctttcc
    12241 aagtaattat gattccgatt accgttatga tgagtaaaat tggtttagca aatatgtggg
    12301 gattaatctt actctatctc acttatgccg taccacagac actattttta tatgttggtt
    12361 ttattaaact aaatgtgcct atcagtctgg atgaggctgc tgaaattgat ggggctagtc
    12421 actttacaac ttatttcaaa attgttttcc caatgttaaa accaatgcat gcgacgacta
    12481 ttattcttaa tgctctatgg ttttggaatg atttcatgct accattatta attttaaatc
    12541 gtgattctaa tatgtggaca ctaccattgt ttcaatataa ttatacggga caatatttta
    12601 acgactatgg tccaagtttt gcttcctata cagtaggaat tgtcacgatt acactagttt
    12661 atcttgcttt ccaaagaagt attatagctg gaatgagcaa tggagcagtg aaataaccaa
    12721 taaattattc tcattataaa ggagttcata aacttatgat tatatttgac aatcacacac
    12781 aaacatttca tttacaaaat aaaaaaatta gctatctatt aggaatcgaa gaaaataatt
    12841 atttaacgca tctttatttt ggaaaaaaga ttacgcacta ttctggtggg tatcgctacc
    12901 cccgaacaga tcgttccttc tcacccaaca atgcaaatgc gcaagatcgt ttattatctt
    12961 tagacacatt accattagaa tttccaggat atggacatgg tgattttaga gaaccggcgc
    13021 ataatattaa attaggaaac ggtagtcgtg taaatgattt tcgttatgat agctacgaaa
    13081 ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt atatacggaa gatgatgatg
    13141 aagctgaaac gctgattatt caattgaaag acgcgattag tggattgact ttacagttgt
    13201 tctacacaat ttatcaaaat cacagtgtaa ttattcgttc tacgcaatta gtaaataata
    13261 gtgatgaaac agtggagatt aaccgcttag ctagtcaatc aattgatttt ccaaatcgtc
    13321 cactcgagtt aattcattta aatggtacct gggcaagaga acgtcaaatg actagagaaa
    13381 aaattcatac agggataaaa gtattagata gtaaacgcgg atcaagcagc catcaacaaa
    13441 atccgtttgt tgctttagta gatgccaaaa cagatgaatt tcaaggagag gcatatggat
    13501 ttaatttagt ttatagcggt aatcacgaaa cggtgattca aaaagatcca tttgaccaaa
    13561 cacgggtcat tatgggaatt aattcattta attttggttg gcaactaaaa actggcgact
    13621 catttcaaac gccagaagtc gtgatggttt atagtaatga aggtctgaat acgttatcac
    13681 agacgtatca tgaattgtat aatcaccgtt taattcgtgg aaactatcgt ttaaaagaac
    13741 gtcccacgtt aatcaacaac tgggaagcaa cgtattttga ttttaacgaa gaaaaaatca
    13801 tgaatattgt cgatgatgcg aagaacttgg gcattgaaat gtttgtttta gatgatggtt
    13861 ggtttggcgg tcgtgaaaat gacttcactt cattaggtga ttgggttgaa acagaaggca
    13921 aacttgcttg tggtttggaa aatttagcca agcaagtgca tgataacggg atgaaatttg
    13981 ggatttggtt tgaaccggaa atgatttctg aagatagtga actatttcga gcacatccag
    14041 attgggcatt gggtattcca ggtcgtgacc gctcaattag tcgaaatcaa tatgttcttg
    14101 atttttcaag acaagatgtt cgtgataata tctatcaaca aatgacggcg atttttgatc
    14161 gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa tatgacagaa gtctattctg
    14221 tcttgttgga ctcaacgatg caaggtgaag tggctcatcg ttatatgtta gggttatatg
    14281 aatttttaga aaagttaaca acgaattatc caactatttt atttgaaagt tgttcgggtg
    14341 gaggcggtcg ttttgatgca ggtttcttgt attacatgcc acaaacttgg acgagcgaca
    14401 ataccgatgc cgtggctcgt ctgaaaattc aatatggaac cagcttagtg tatccaattt
    14461 caagtatggg tgcgcatgtt tcagcagtcc caaatcatca aacaggaaga gaaacgagtt
    14521 taactattcg tggcaatgct gcgatggctg gtgtttttgg ttatgagcta gatctaacgc
    14581 aattaacttc tgaagaaaaa gcagaaatta aagaacaagt tgccttttat aaaaagcatc
    14641 gccaactttt gcaatatgga actttttatc gcttggaaag tccgtttgaa ggcaatgata
    14701 ctgcatggat gtttgtctca aaagaccaaa cagaagccat ggtcttttat ttccgagtat
    14761 tagcagaagc tgcgcatcca ttagtcacat tgaaattagc aggattagca gaagaagcaa
    14821 cttatcaaat ggaagatgta acatttgctg gcgatgaatt gatgaatctc ggtttttata
    14881 ttgactcaga actacatggt gattatgcga cacaacgctt ttatttgaaa cagatatagt
    14941 agcttgaatt tacaatataa gtgcaacacc aaaagagact cattccaaac gaagaaaata
    15001 tgttttattt acacgcattt actaaaaagc agcctgattt gaattgggaa aatgaagaag
    15061 tacgtgaaga aatctatgca atgattaact attggttaga taaaggacta ggcggattta
    15121 gaatcgatgc gatattaaac atcaaaaaaa aactagagta tggcatcttt gaatctgatg
    15181 gagaagatgg gttagccttc attgggccct ggattcttaa tcagtcgggc attgaagtgt
    15241 ggttaaaaga aatgagagag cggacattta aaccacataa tagtatgacg gtagcagaag
    15301 ccgatgttcc taatgaacgt ttagatgaat atattggtga agatggctat tattcgatgg
    15361 tttttgattt tagttatacg gatattgatg tacctgaaac tggtgaatgg tttaaagata
    15421 gccaatggac atggacggat atgcgtacca atatttttac gaatcaatta gttacgcaag
    15481 ataaaggttg gggcgcctta tatttagaaa accacgatca accacgttca attaataaat
    15541 atattccaga agaatggatc aatgattata gtaaaaaaat gttggcgacg ctttttatgt
    15601 tactaagagg tacccctttt atttatcaag gacaagaact aggaatgacc aacatagaaa
    15661 tggcatcatt agaagatttt gatgatgtag ctacgcattc tcagtataaa cgggcattag
    15721 aatatggatt ggcaccagag caagcattga aagctgtcaa taaacgaagt cgggataatt
    15781 ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag attctcaact tctgataatg
    15841 tatggttaaa agtaaatcct aattatccag aactcaatgc agaagctcag ttaaccaatc
    15901 aacagtcggt attcaattat tatcatcaat taattaaatt aagaaaaaat agtgtataca
    15961 aagacgtgct aatctacgga caatttctac cagtgaaaga taaaaatgaa catattttgc
    16021 tttatgaacg ccaattagaa gataaaatcg tattagttat cttaaacgtt acagcaacag
    16081 aacaagaata ttatgtagat gaacagtacc aacaagtatt aatcaataat tatgaagatg
    16141 ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt tgaaagtatt gtattagcta
    16201 attatggagg acaattatga aaattaaaaa cgaagcaatg ctcatcacct atgcagatag
    16261 tttaggaaaa aacatgggcg aattaaaaca agtcttagac aaacatttac aaggtgttat
    16321 tggtggtgta catctgttgc ctttttttcc atcgacaggt gatcgaggct ttgcgccgag
    16381 tgattatacg acagttgatc cagctcttgg aacatgggaa gatgtggaag cattaggtga
    16441 agattattat ttgatgtttg attttatgat taatcacatt tctcgtgaat caaaattttt
    16501 ccaagatttc aaaaaaaacc atgagcagtc accatacaaa gaaatgttta tccgtattca
    16561 tgaatttttc ccagaaaatc gaccaacaca agaagatatt gatttaatct acaagcgtaa
    16621 agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt acgacagaag aggtttggaa
    16681 tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa gaagttgtga aagaatttat
    16741 tcgagaaacc atcaaagata tggcgaacca tggctgttct ttaattcgat tagatgcgtt
    16801 tgcttatgcg attaaaaaat tagataccaa tgatttcttc gtggaaccag atatttggga
    16861 acttttagac gaagtgcgtc aagaagcagc gaaatatgac gtcgaattat taccagaaat
    16921 ccatgagcat tattccattc aaatgaaaat tgccaatcat gattattata tctatgactt
    16981 cgccttgcca atggtcactc tgtattcact ttatagtgga aaatcagaac gtttagccaa
    17041 ttggttgaaa atgagtccga tgaagcagtt tacaacattg gatacgcatg atggaattgg
    17101 tgttgtggac gctcgtgatt tattaacaga tgaagaattg gattacacct ctgaggaatt
    17161 gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt gccaattaca ataatttaga
    17221 tatttatcaa attaacagca cctattatag tgcattaggc gataatgacc gtagctatct
    17281 gttagcacgt gcgattcaag tatttgcgcc agggattcca caaatttatt acgttgggtt
    17341 attagcagga aaaaatgata ttgaactgtt ggaagcaacg aaagaagggc gtaatattaa
    17401 tcgtcattac tatgatttag ccgaaattga agaagaagtg caacggccag tgattcaaca
    17461 attatttgca cttttaaaat ttagaaatga atctgcggcg tttgatttgg atggaacgat
    17521 tgaagtagca acgccatcag aatcagaaat agtgattaca cgtaaaaata aaaacggtag
    17581 tgatagtgca acattaacgg ctaacttgga tacgaaagaa tttatcattg agtctaaaga
    17641 tataaaaatt ttcactagtg ttgcattaaa attttaacct attccataga acaaaaaatc
    17701 ctttatacta gcacttggat gacttatcca agaccaataa aaaggactca tacatggata
    17761 agtataaaac aaatacagca tttaataaat ggttttctgc cataaaatta ggaaaacttt
    17821 ctgtcaactc ccaaaaatgt atttttgatt acaattcgta tcgtaaaaaa ctgtcttttg
    17881 aagctgtgct caagctcttt ctctacgcag ttaatgaaga aaaagaaagt ttgcgtgatt
    17941 tgagtacagc tcttatcgac cgatcgctcc aactagaaac cgatgtgtca tcgattagtc
    18001 atacccaatt gtctcgagca tttaatgcac ttgaacctaa agtgttagaa gaaatttttc
    18061 aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac caagcgaaat catctgtatc
    18121 tgattgattc cagtacgttt tcgcttagtt taaaacgtca taagtgggcg actttccgtc
    18181 aaacaaaatc aggtgtcaag ttacacttaa actattgtta tatggacgat actacgatgt
    18241 atccaactga ttttacctta acaaatgccc atgaacatga tctcaatcaa ctaccagtat
    18301 tggtgaatca accagaggca acttacgtgt ttgatcgtgg gtattaggac tttgaaaaga
    18361 tggatcagat gcattgggat ggctatttct tcgtcactcg gattaaaaaa aacaccaaag
    18421 ttcacgtgat agatactctg gaaacatcgc cagaaacaga aattctacga gacgagctgg
    18481 ttcgtttagg atctaaaacc tatctaaccg ccaatttccg actagtcact gttcaagata
    18541 agaacggacg tgtgtttcaa tttatcacca atcgaatgga cgtctcttca aaagaaatct
    18601 ctgatatgta tcacgctcgt tggcaaatcg aattgttttt caagcacatc aaacaacaca
    18661 tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt gcagaatcaa ttgattttaa
    18721 cgatgatttc cgctctcctc acgtttctca tcaaattgga gacgaaaacg gaaaaatcag
    18781 tgttccaaat caaacgattc tttcgttatt tactgtttca gccctttgaa tgttgtctag
    18841 aaaaattaat tcccacatga gaaaaaagaa atttggctcg ttacttttgg ttttcagttg
    18901 aataacagat aaaaaatttt ctggaaaaag tcatcctctg cgtcagcaca gttttttttg
    18961 acttattttc agaggaaata gttttcagaa tattctaaaa tgttttatgc aacactagtg
    19021 atatttttta gaattagatt tctaatgtgc attttttgag tattaaggag caagtataca
    19081 aagatttata aagctgatta aatttgaata aaaatatgca ggaacgctac tatttttagt
    19141 agtgttcttt tttatatttg aataaaaaat gtggtggtta aatggtggtt ataacaaaaa
    19201 atgaattaat gaaattaggt tatgggaagt atcagtctga gagaatcata cgacaggcaa
    19261 aagcaattat ggtttcaaaa agctatacct attacgaaaa tagaagatta ggactagtac
    19321 ctgttgaatc tgtagaagaa gttttaggta tgaagataga ggtagaggag gattttcaga
    19381 atcatggtta attttaaaaa agataaaaat gggaaatatc attttactgc tagtttggga
    19441 ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag aggggttaaa acagttaaag
    19501 aagaaaaaaa gcgtataccg aacttgtttc taattatgga aaagaagcag aaacttctca
    19561 aagtactatg ttgtttgagg agttttttta taaattattc ctaccttatt atcaagggaa
    19621 agtagaagaa agaacatata aaaatcggtt atctacaatc aaaaaaactt ttcttatttc
    19681 tttaaaatgg agttaagaga aatacgtcca attgacattc aaaagtggca aaataattta
    19741 ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg ggatttttca gatgtcatta
    19801 gatcgagcag ttgttttggg actacttgaa aaaaatcctg caaaagttat tggaaatgtg
    19861 aagaaaagga aaactacagt tgatttttgg acaaaagaag aatttacaaa agttatttca
    19921 actttttata ttgatgattt gtaccaacat tttggtttta tttgtatgtg gttacttttt
    19981 atgaccgtta tgcgtttggg agaagccact gcattaactt ggaaagatat tgattttgaa
    20041 aataaaaact tgcgtgtgaa taaaacactc tattataaga gagccaaaac ctatgagttt
    20101 gtagaacccg aaacggcttc tagtattcgt acaatcgaac tagacgacaa tacaattacc
    20161 tttttaaaag cttggaagga gcgtcaagtt tcaataggag gcatgtcgtt tgtactttct
    20221 tataatggaa cacctactca gaaatataca atccgtcata ttatgagaag acatgcaacg
    20281 atcgttggtg ttcatataat ccgcatacac ggattaaggc atagtcatgc ttctttgctt
    20341 atatcacaat aaaaagatgc acttcgaatt aaagaaaggt taaaacatgc tgatgtacaa
    20401 acgactttag gaacttatgg tcatttgtat ctaaattcat tgagagagat aacaaatgat
    20461 cccacagatt atatccaaac taatttttct aaaatcaaac cgacacctat ttacgataat
    20521 aaaaaataaa tgataaacaa aagtaggatc acaccaaaag aaacacacta gaaaaactat
    20581 aatacaggct tttctagcgt gttgtttctt atttccgcct atttatacat tatactcata
    20641 aagcatttaa tgcatcgatc gtagccgcat tgtctatatg ggtatataaa gttgttgtat
    20701 tggtacttga atgtcccaat tgttgggcag ttaataccaa tgaatttgtc tgttggtaga
    20761 gtctagtagc taatgtatga cgcagtttgt gagggctgac ttgaattcta aagccttttg
    20821 aatattttgc taccatccgt tcgattgcaa taccggatat acgcttggct tctccttggt
    20881 aaattgctag aaataaagct ttttcgtttt ctgttacttt atatcgttct ttccgaatag
    20941 ctagataatt agctaaatat tccattgcga atgctgctat attgacagca tctcttttat
    21001 ttccttttct agttactacc acattattct tatctaaaga taggtcatct aaattaatat
    21061 ttataacctc ggagagtctc aatccagatc ctaaaatcag tgcattgatc gccaggtctc
    21121 tttctacatc tcgttgataa tagatcaaag ctcgtttact caaagtattt ttatattcat
    21181 tggcaatata atctagataa ttttgtgtat ccttatctag aaataatttt ttctcaatgg
    21241 cattggcacg tgcagctaac gtagttttat ctgtgatggt tgctacttga tccataggat
    21301 tattgtctaa gtatggtttt cctagaggat tattggaact tttcgaaaga tatttaaata
    21361 gaacctttaa tgccgtgata tttctattga tagtagaagt agatagacct aagttattat
    21421 tatctttgag cttagttttt gttgtctctt tttttacatt ttcttttgtg cgacgtttca
    21481 aatgagattt gtacaagttt aggtcatagg cagttaactg tcttatcgag gtgatagaag
    21541 tatcaggaac gatagactca ctcagaagcc attcataaaa gagcttataa ttttttagat
    21601 attctaaagt ggttcgaggt gattgatctt tatcctcacg atcttgaaca aaatctttta
    21661 tataatcggg catatgaagt atgattgtat ctattttttg atatatttct gtatttttca
    21721 tgtcattcat cctttcaata agtaggggag aagagtatct ctataattgg actataccaa
    21781 ttatagaatg ttttgtcaat tggtatgctc taaaatttat taatttgttt caaaaaaagc
    21841 atataaacag gtatacgttt aataatttaa aataaatggt atacataaat agggaaggaa
    21901 atagaaggga gcaatctaaa ttttttgaat tttcttaatt aattatatag aaagaacaat
    21961 ctttctatag agttagacat gagagggact atgcttgatt taaaatcaac aagcgtatat
    22021 actgtatata caaagaaaaa ggagggagaa gactatggca attaaagaaa gaaaatttag
    22081 ggaagtgggg aactcagtag tattaactat ttcaaaggag tttcttaaag aaaatgggtt
    22141 aaatcccgga gatacggttt ttttagatga agaaaaactt cgagcggcaa ttacaaaaaa
    22201 acaagcctta tctgatgaaa ttgatttagc aatatctcaa gcattaactg aatatgatac
    22261 tacttttaga gagcttgtcg ataaatgagt attcattacc tagaagccaa agatattata
    22321 aaaatgaatg taatccaaat aaaaaaatac tctcctaatg aaccccttgg tgtaaaggat
    22381 gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct cagcttttgg tgaagaggtt
    22441 tatccttcga tttatgaaaa agcagctatc ctacttatac aattgataaa gaaacatcct
    22501 tttcacaatg caaataaacg tactgctttc ttagctactt ttgtcttttt gaaaatcaat
    22561 ggacagcttt tagctattga tcaaaaagat gctgtaaatt tagcggtata tattgctacg
    22621 tatgataagg atttcgatca actaaaatat gaagtgatca acactataaa gacacatacg
    22681 cagcccattt aaggagttac tgtatgtgtc tttatagggt tattagctgc tatagcgaaa
    22741 taattgaagt aaattacaaa tagctataat tcaaatccaa ctatatagaa aacgtaaatt
    22801 ttctatatag ttggattaag ataacgacta tt
//
LOCUS       DACDSR010000039        22174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDSR010000039 DACDSR010000000
VERSION     DACDSR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.039"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..134
                     /locus_tag="IWK15_001519"
     CDS             <1..134
                     /locus_tag="IWK15_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9547113.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(205..1515)
                     /locus_tag="IWK15_001520"
     CDS             complement(205..1515)
                     /locus_tag="IWK15_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9547114.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1527..2945)
                     /locus_tag="IWK15_001521"
     CDS             complement(1527..2945)
                     /locus_tag="IWK15_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9547115.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3185..4753)
                     /locus_tag="IWK15_001522"
     CDS             complement(3185..4753)
                     /locus_tag="IWK15_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9547116.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4757..5038)
                     /locus_tag="IWK15_001523"
     CDS             complement(4757..5038)
                     /locus_tag="IWK15_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9547117.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5035..5958)
                     /locus_tag="IWK15_001524"
     CDS             complement(5035..5958)
                     /locus_tag="IWK15_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9547118.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5955..6833)
                     /locus_tag="IWK15_001525"
     CDS             complement(5955..6833)
                     /locus_tag="IWK15_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9547119.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6820..8157)
                     /locus_tag="IWK15_001526"
     CDS             complement(6820..8157)
                     /locus_tag="IWK15_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9547120.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8160..9263)
                     /locus_tag="IWK15_001527"
     CDS             complement(8160..9263)
                     /locus_tag="IWK15_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9547121.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9337..10845)
                     /locus_tag="IWK15_001528"
     CDS             complement(9337..10845)
                     /locus_tag="IWK15_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9547122.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10811..11503)
                     /locus_tag="IWK15_001529"
     CDS             complement(10811..11503)
                     /locus_tag="IWK15_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9547123.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11525..12499)
                     /locus_tag="IWK15_001530"
     CDS             complement(11525..12499)
                     /locus_tag="IWK15_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9547124.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12799..13671
                     /locus_tag="IWK15_001531"
     CDS             12799..13671
                     /locus_tag="IWK15_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9547125.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14253..16451)
                     /locus_tag="IWK15_001532"
     CDS             complement(14253..16451)
                     /locus_tag="IWK15_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547126.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16683..17675
                     /locus_tag="IWK15_001533"
     CDS             16683..17675
                     /locus_tag="IWK15_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9547127.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17735..19156
                     /locus_tag="IWK15_001534"
     CDS             17735..19156
                     /locus_tag="IWK15_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9547128.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19149..20648
                     /locus_tag="IWK15_001535"
     CDS             19149..20648
                     /locus_tag="IWK15_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9547129.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20687..22081
                     /gene="uxaC"
                     /locus_tag="IWK15_001536"
     CDS             20687..22081
                     /gene="uxaC"
                     /locus_tag="IWK15_001536"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9547130.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6726 a         4518 c         3841 g         7089 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atatatcatt aaaaaaaata aaggtcaagc taaaatatta
      181 tttaagcttg accttattat aatttcaacg attgtcaaaa gtattgtttg cagctgtgtt
      241 gccagtttct atagataaat cacagctttt gaaacataag ttttgtactg cttcgattcg
      301 cccgaattct tttgcagtta tttggcaatg agaaaagcag acatcttcta taggcttttc
      361 aggaaatgct actaaatcaa aagctcggga aggaagtgag taacctggag aaaggcatgc
      421 ctgtacgttt ttgatgtgta tgtctttgac tttcgtcatt tgctttgatt caggtatttc
      481 ttcagccact gcttgccaac tttctggcat ttgagcaagg tcattgctat tttttctatt
      541 gtattcatta tgccagtcca ttatccaaga aaaaggaaat tgtacatcaa tcatattcag
      601 attttctacc ctgatatttt cgatcactcc ccctctttct tttgaagatt tcattcgaat
      661 accacagtct gactgaaaaa aattgatatc gtgaatatat acgtcactaa ctccagcact
      721 aacttcgcta ccaatagtta caccgtatcc tgaacggatt ttacatctag caacctcgat
      781 tcttgatgat tttcgattga ctcttgcacc attgccatct ctgccagact taatagcaat
      841 acaatcatca ccacaggcca aatcacattc atagacacgc acatttgttg aagaatcaat
      901 atcaatccca tctgtacttg gtccatcatt gttgcggatg attacttttt ctactaatac
      961 atcgttagaa taagtgattt gcaagttcca aaaacctgat ttctgaaaaa tcaaatcacg
     1021 aacaacaaca tgttctgcat gatacagtaa acaagcgcgt ggacgtttaa tagcataatc
     1081 cgcaatccaa cgaagtccct tttgatcgta ttcagctctt gtcccgcttt tttgatcttt
     1141 tccccaatac agctcccacc aatggtctcc ttgaccgtcg attattcctt ttccttctat
     1201 aagaatatct ttcgccgaga gaacattcaa aatcgctgct ggccatttca tttctacgcc
     1261 ggctactcgt gtatcgattt ctggaaatgc ttcgatatct gttctcccct tgaggactgc
     1321 accttcttca aagataagat gactcttatc ttttaaaaaa agggctccga ctaaataaat
     1381 gccttttgga ataaatacag gtaagtgttc tttcgcacct tgatcgattg ctttttgcag
     1441 tattattgta tcatcttgtt gagtgtcgtt tgttttaaag ttcaaagcat tgataatccc
     1501 tttttttgtc cgcaatacgt ttctccctat ctttcattga tttttttcaa ttcttcacca
     1561 ataaatacag tttttgaacg attcagtggg taacctacta gcaaaatgat tgcggataca
     1621 aataagcaga atgctggtaa caagtttgcc atcgtataaa tcctttcttg tactaaaact
     1681 gattggatgg ctccacctgt tgtagacgat tggtaaccaa tgagtgccag cataaatccg
     1741 cctaccgctc ctgcacatgc ttgtcctact tttctcgcaa aagaatttac tccatatact
     1801 gttccatctt ctcttaagcc tgtcttatat tgatgataat caatcacgtc agtaatgaat
     1861 gcccagacca tcaaattgaa taaaccagat cctaaagtag cgataaataa aagaatcaga
     1921 tatacccatg catttgttat atgaaggaaa tacatcagca agtacataca tgaagaagca
     1981 agtaacgcaa atacgcttgc ttccttttta ccaaataatt ttgtcagctt tgtagcaaat
     2041 ggtgctaaaa ctactactgt tccgtaagta aataaaagag caacagacat tgcttgttta
     2101 ttatggaagt aatcattgaa taaataagtc atattcgtcc cagctaaaat ctgattgatc
     2161 acaatgaaaa tgtcaacaat gactaaaata atcagtgctc gattagctaa caaacctttt
     2221 accaactgag aagcagatac tttttcgttt ttgacaactc ttactctttc agtagtcaat
     2281 ttacaagtta acatatatgc gacaaatgct attgcagcac atccgattga aatccaaaag
     2341 aaatgattcc ctgaaacgac ttgttgtcca ctcttgttat gggtatacat taaaatcgga
     2401 atgaagaatc ctgtagttga tccgccaatc gcagaaccaa tgcttcggaa cgtggacaac
     2461 gaggtgcggt cattaggatt tgagctaatt gctgctgcca ttgctccata cgggatattg
     2521 attgttgaga gaaaaatccc ataaacaatg tatgaaacaa aaatataagc aatcttcacc
     2581 gtcattggaa gatgttggac gaatggcaaa aacaaaatga cagtcactaa gcaaaatgga
     2641 tacttcattt ttctgatcca aggaataaat cggccagttt ctgttaactc tgagacatca
     2701 cataatcttc caaccatcac atcagcaaac gcatccaaaa ttctcgaaat caagaataac
     2761 gtacctacga ttgcccctga aacgccaaga acattcgtat aataaatcat caagaaacta
     2821 ttgactaaac ctaaaataaa acagttacct agatcaccga acatgtagcc tatcttatcg
     2881 gaaatcccaa attccttttt ttcttttttt actgtatttg ctgtaacagc attttgtgaa
     2941 agcatttgaa ttcctcctac ctcttctagt aaacgttttc aaaattataa ctcttttttt
     3001 cttgttttta tattcacaat gttggctttt tattgcatta tcttgacaga taaaaaacaa
     3061 aaaataatct aaaaacattg atgcataaga ttgataactt tttttacata acaatatata
     3121 caaacaaaaa aattcactaa ctagtgaaaa taagttagtg aacctcattt attaaagaat
     3181 taaattacag atgatctttt ccatcatact tgaaaaactc aaaatcagca taacttcctc
     3241 tttttttatt tagatcatgc actgctatcc cgataaaatt tccagtaaac cctcctgaga
     3301 gtatacacat gtttttttgc ggacctactt tcttccattc ttcagttgat acgtctttca
     3361 aatgaaactg tgctgtctct ttctttacag tgactttcaa aaaagtcttc ttttctgtga
     3421 gtggaatcgt ccaatcttct atttgtgcta ctccgttttc tgattctatc atacgtaggc
     3481 attttccctt gtcctcattc caagtaacat aacaataaat ataattggat tcatttaagt
     3541 acagtaccag tccagccatc tgattaaaat tattaggcat gtattcaaat gctgtctccg
     3601 catcaaaagt aaaatccgtt tgccgaatag ctaacaaatg atgttcgaag gtagattgaa
     3661 gagattcacc ggaaatcaga cgtaaaaaac ctggtctaca ttttgtatca caccaatcag
     3721 catttggcaa aatgcgtctg gcattccagt tcttctttag ctctccttca aatgtatcca
     3781 taaactgatg atctttttct tgttgtacct cttcatttgt ctcaatcatt atttcttcca
     3841 tcggagttgt ctggctatct ttaagtctta accaatcgtc ggacgtccaa tacacttctt
     3901 gaatagcggt ttctcttccc aaaatagccg caccgtttaa tggtctggta caaagatatg
     3961 tcatgtacca tcttcctgac ggggtttgta ctaagctccc atgtcctgta cattgcaatg
     4021 tagaagcagg tttgtcacta gctgtcagca taggaaaacc aggatcaagc tcgtaaggac
     4081 cagtaatatt ctttgctcgg caaactgtaa ctgaatgtcc tcttcctgta ccgccttctg
     4141 ccgtcatcaa ataataatac ccgttacgaa aatacaaatg tggtgcctcc gttttcgcta
     4201 attctgtacc tgcaaatatt ttatagatgg gaccatcaag cttcttcttc tctgcatcat
     4261 attcttgtaa tacgataccg ctggatttat tcggtgtgtc cagacgataa tcccatagtt
     4321 cattgacaag ccattttttt cccgtttcaa gatcatggaa caaagaagga tcaaagccgt
     4381 tactatttaa ataaattggt tcggaccaag gacctgtgat ctcagatgca gtgatcaaat
     4441 aattgtggac atctttaaac ggacggcctg cacttttgac atctgtgtaa acaagataaa
     4501 acaatccatc agcataactg atctgtggcg cccatatact tccatttgta ggatttcctt
     4561 gcaaagatac ttgagtggtc aaaatatccg ttgtatgtga ccagttgact aaatttttcg
     4621 actcatatac tcggattcct ggaagccatt caaaagagga gacaataatg taataccgct
     4681 ctttcactct taatatatta ggatctggat tgaatcctct taaaatagga tttttcactt
     4741 tgatagtctg catgattcag gctccttttc tcattcttac tcgatataaa ctgaatgccc
     4801 cagctgctaa aaaagcaagt gaaataaaca acacaacggc aaacagacca gtcatgtcct
     4861 gtaataatcc aaatgtattt tctaaagaca aagttgaatg atctacagca agcgactgat
     4921 aacttttttt aaactctgac gcagacatat tacttaaagt aactgctaac ccgccaaaga
     4981 aaatcaaaga tagactgcca ccgaccattc ctagcaatag ttcccatttt cttttcattt
     5041 ctgaaccccc tgcttttcta aatgtatcca ttcaggtctt ttgatcgatg tttcataatt
     5101 gttattacgt tcagagaatt tgacagtctg ttcatttttc ttgtcatccc aattatccca
     5161 accagttggg tgaacatgcc cacctatctt acacgcttca aataagacat ttgcatacgg
     5221 acgccacggt cttcctaaat agacgtttct agtatcaggt tctgcagtca aggtacagct
     5281 ttcaaaacga taccctgttt tttgattttt cggcgtagat gctgctgtga tgtatccagg
     5341 atttttactg gctaagctat ggatatgaca ctgatgaaaa acggcatctg ccccaccaaa
     5401 tatgaaatcc accgttcctt caatccagca atattcatag gtctgacgac aatactcagg
     5461 attggcagaa gccggtgtac aaaaaggagt accatctttt tgcaaatcag gcaacggacc
     5521 cgtgcataag gtatcctgat aaccagatag tcgacagttt tttaaagata cgcgatctcc
     5581 tgaattgaat aaagcaacag cctgtcctac cttttctcct ggacctgctg tattttcgat
     5641 gtgaagattt tctaatacga cattcgatcc ttctaagaaa aaagtagcac ttcggaaagt
     5701 ccctaactgg ctaccgtcaa gatactcctg ttttgcaaaa cgattaccgg agataactac
     5761 tgttcctgct cccacaagtt taaaattgga taatctgctt tctatatatt catcatatct
     5821 tccttctttt actatcaaga cctttggatg gctatccggc aggcctgcca aataatcgat
     5881 tccttcttgg atagtttcaa aatccgtcgg ggtatctata gcattctttc caattatcag
     5941 tttgtctttc atcctcataa atgctccctt atgaattgtt tccaaatatt tctcatttct
     6001 tgtgtttcca tatgaccgcc tgttaaagaa aaagaagaaa agttttctgg actacccatt
     6061 gattgatact ttttgtccag cccttttctc agcttcagta cgccttcgtc caagcacatc
     6121 gtatcgtttt ttccaactaa gctaagacga gggcgcggag cgattttttc ttggattgct
     6181 aacgttgtat aagctgtcaa tagatttggc acatagtaat aaaacccatg atgatctagg
     6241 cggcgatgta gcaaaagtgt ttcgatgtcc acctgtgccg ctagatcaat acaaactttg
     6301 acacgagaat ccaatgctgt cacccaccag cttaataagc ctcccataga aagaccaatc
     6361 gttgcgagcc gttgagtatc tgtgtcttcc cttgtctcca aataatcgat caagctgatc
     6421 acatcataaa tacgcattcc ccataacgtt tttcctgtta ataaaaattg tttgaacaac
     6481 tcgctttcag aaatacctcc gcgttcttca aatccccaag catcaatcga ccaaacagca
     6541 tatcccattc ctgtcagtac ttctgcaaaa gatggagaag ctaagtaagg agcaccatgg
     6601 atcaattcac ttttcccgac tgaaaaatct ccgccgtgag aatgcaggta aaggaccgtc
     6661 ggaaacggtc cttctttttc tggataagca tagagggctg gtactgtttc caattcattt
     6721 aagtacaatt gatagtattc aagcataaaa ccgttttgtt tttctcggta taatcgtttt
     6781 ccactagggg tatgtctgct agatggagca cctaaaaaat cattcaactg cataagcaaa
     6841 gtttttacga acaaccagtt catccatatt ttctgattga tttgattgga atttctcaat
     6901 actgatttct ttagtatttt ctaatagaaa tgctggtccc tcgtgatttt caatcgacat
     6961 ctggttaaag gaaaccttac ccgcaaagcc aatataaaat ccttgtctgc tcatttcctc
     7021 tattcctgtc atcatagcag gaacaccggg aactgcatct tgggccatcg agacatcgat
     7081 attgtcaaat gtgatttctt gtatgaattg ttccgctaat ccataaataa aacctgcaga
     7141 agcatggaca ttccttgccg tgatattcga aaaatggatc cttctgaatg ctgatgtccg
     7201 ttcatctatc ggataagcct ttttttccca tacatacggt tctttccctc gcggaccaca
     7261 gaaataataa agattcagta taaatggaca catcacatta tccatgacta tattggatac
     7321 tcgaatatct tcaacgatcc cgcctcgacc tcgtctagat ttcaatcgta ttcctcgatc
     7381 agtttcttgg aagatacaat ttgaaatggt aatattacga atactaccac tcatttcact
     7441 tcctaatacg acaccgccat gtccatggac catcgtgcaa tttgtgatcg tgatattttc
     7501 gcaagcgatc ctttcgtacg tatcttcagt ccctgccttg attgcaatac agtcatctcc
     7561 cacgtcaata tggcaattac taatacgcac atttttgcat gattctggat caattccgtc
     7621 tgtatttggt gaatccgctg ggttcaagat cgtcaagtta tcaaacgttg cattactgca
     7681 aagaatcggg ttgatcgtcc aacttggtga ctggatcaat ttaatatctt tcacagtaat
     7741 tcgatgacag ttatggaaac tcatcaattt aggacgagga taagcgagat tatctggttc
     7801 atttctaaaa gtatgccacc atttttttcc atttccatct aatgtaccga aaccagtgac
     7861 agagatgttc tctacatttt gagcatagat acaggaagca taaacttttc gatggactcc
     7921 ttcccaacgt gaatgaacaa caggataatc ttttggatcg tcagagaatt tcagaaccgc
     7981 tcctgcgctt aaatgaagtt cgacattgct ttttaaaaat aaagctcctg ttagaaattc
     8041 acctgctgga acaactacgg tcccgcctcc gtcacttgct gcagcatcaa tggcttgttg
     8101 tattgcttca gtattcaatt catcaattga tgcgccaaat gtcaaaatat catacatatt
     8161 tataatgcct ccacttcaat caatgcttgc aagaatgcac caacgccttt ttgatcatta
     8221 caaataattg gttcactgat gtagtaagca tatgaaccat ctcgctgatc tgctccgcct
     8281 aaccctgcta cttgacagtt tttattcaag ttcagccagc cttcttttgt ttccagaaca
     8341 aattcatcaa gtaatccttg atgtgatttc ttcacttgtg gaagccattc ttctttgttc
     8401 agcagtccca aacgtattcc tttagccatt gcgtaaacaa acatactact gccggaagct
     8461 tccagataat ttcctttccg ttttgtttct gttgttactt gataccatgt cccgctgttt
     8521 tcttcttgat atggaagtaa tgcagccagt gtcttttcta gaatttcttt cagcaaagaa
     8581 caatctgttt cttcatgaag taattcatag gtatctgctg cagccatcaa ataccagcca
     8641 atcgatcggc tccaaaaatg atgggataga cctgtttttg gatcagccca tggttggacc
     8701 ttcttttcat cccatgcatg aatcagcaaa tttgtttttg gatcgaatag atgtttataa
     8761 ctgatttcaa actgtctcaa aacatcttgg taatcttttt cttcactaaa attctggata
     8821 aattcagcat aaaaaggaga acccatatat aagccatcca accaaatttg ataaggataa
     8881 atttctttat gccaaaaagc tccttcggat gtgcgtggat ggtttgccaa ctgctgggaa
     8941 aggagttctg ctgctttttt atatttttct tcgcctgttt ctttgtacaa taaaaagaaa
     9001 agctttcctg tatttacatg gtcgatattg tattcttcaa cgctgtagcc acggatcgtt
     9061 ccatcttctt gcacgaaata gtcgatattg tcttttatga aatcaaaata aattttttct
     9121 tgggttcgtt cccaaagtaa ttggaaccct ttaagtacga cgccatagtc ataagaccat
     9181 tttcctttat accacttttc ttcatataag cgaggtgttc tttccatgat actttttgct
     9241 aatttctttg accagttttc cattatttgc tctcctttaa aaattaaaat aggatttggg
     9301 acaaatacca agaagtattt gtcccaatcg tctatattac tgtgccgctg ctttgtatgc
     9361 ttcctcgtac tcttgtgtga ttcgatcgcc tccttgtgct ttccagtctt ctacagcttt
     9421 tttgaatcca tcttcgtcaa gctgtcccat gatgtattgc accgtagcat caatgacgat
     9481 tttttcaatt tctgatccta cttcattgtt cgtcggtgaa tccaatggca cagaaggatc
     9541 cattacagca aattcttcat tttctttgat caattgatta gagaattctt tttcagggtc
     9601 tgtatctttg aaagagaacg tcacttcact aggacgagag cttgataacg gttgcacctc
     9661 ttgttgccaa agatccatat cgataaattc gatagaacca tcatcattca attcataatg
     9721 cgttccttcg ataccgccag tcatcaattt atacacatct tcttctaaaa gatcatcaac
     9781 aaattgaagt aatttcttca attgtgcttc gtcttttact tgagatttgg gaaatgctag
     9841 caagccgcca ataccatttc cttctgacca aacatgataa ccgccgcctt caccatctga
     9901 gatcttgttt acaggcacta attccatatc atcttgaata ccttgaccca tcgtcttcag
     9961 attcttgata tccaccattc cagtgtagat tccagttctt ccttgtgcaa attgttcttg
    10021 ctgatcagtc ttttgggtaa ctgcaaagtc ttgtgccaaa tagccattct caaatagatc
    10081 tcttgaaaaa ttcaaagctt tcatatactc ttctgtatca aattctggag tgaatttgcc
    10141 attatcgtct acagcccagc catttggtgc tccaaaataa gaagtgaaaa gtttaaagct
    10201 ggtataacga atatcactag gcgcacgatc gccaaaacca actgtgtcgt ccacaccatt
    10261 tccatcagga tcgttttctg taaattgttt agctacttca taaagctcat ccatggtagt
    10321 tggaacatct agtcctagat tatccaacca gtcttttcga atgactaagc cagcacgagc
    10381 gtagtctttt tgtatcggta cgccgtacaa ggcaccgtcg atactcgcag cttcgattct
    10441 atcttcagaa atttgactta aatttttata gtcttttaaa tattctgata catcccagaa
    10501 tacgcctgat tttaacgcat tgcggacaga agagtttttc aacatcgcaa tttgaagtgt
    10561 aacgatatct cctagttcat cagaagccaa tgcagttgtt aatctttctt cttttgaagc
    10621 atcaggaatc cagttgaatg tcagttccgt atcggttttt tcttcgatca agttcacaat
    10681 cgtgtctgta ggcggtgaag catgatgcaa aatatttaac caagtgattt tagcttttcc
    10741 atcttctgtc tctgaagaac tgttacttga actgctgccg catgcggcaa gtgctagaat
    10801 tcctgcaaaa ctaagaccta attttacgag cgttgctttt ttcataaatt ttctccttca
    10861 ctgttttaat aatatttttg ttttcttctg ttttttctgt atatgagttc ttccattcca
    10921 aatggcaaaa tatagaattt gctgtccacg tgactgcgga aaagaagaga ctgccaccga
    10981 taaaggttaa aacacctggc attgtttgga tgatcatcag ataaatccct accatactta
    11041 atatcaacac taatgagtaa tgaagatgtg agaatccgaa taaaaatgcc attttcattt
    11101 tgaaaaaata tcctttccaa tcataatgag ccatgattgg gaaaatatag agtgctgaga
    11161 gcacatatat gaacgcagca atatacaaag cgatcctaag ataccaattg tgtatgaaag
    11221 aaagatccac aagtaaaaca atcccgatgg cgccatatat ccagctcatc agcaaggttt
    11281 cacgatagtt ttctttaaaa tatgtgaagt acgttttcgt caaaggaaac gtttcttttg
    11341 agcgcagcca ttttctaatc actgcaacca tcgcatacga tgcaggaccg aaagtaaaaa
    11401 ctcccaaacc tgcgatgact aaaccgcccc acagaaaatt caaataaacc aactgtaaaa
    11461 tacgatttag ccttccgttt attttatcta atgactgcag catatctttc cctctctttc
    11521 tatcttagta aacgccgtct tctcctagct ttttggcaat cttattagct aaaattacca
    11581 taaacaatcc tacgacccct ttaaacaatc ccattgctgt actgaaactt aactgtccat
    11641 tcttcaatcc agctgtataa atataggtgt cgaaaatttc tgcaacgctt cgattcaatg
    11701 aattcaataa caagtacata tgctcgaacc ctaagtctaa tgtctgaccg atttttagaa
    11761 tcagcaaggt aatgattact ggacgaattg ccggcaatgt aacatgccat gttttgcgta
    11821 agcgtcctgc tccatccatt tctgcagctt catacaactg tgtatcgact gcagttatcg
    11881 ctgaaaggta gatgatcgta gaccagccca actctttcca gatcacttgt ccgatatata
    11941 ctgtgcgtgt ccattcaggg gatgttagaa aactgatttt ttcaaatccc attgttgcaa
    12001 ggaattcatt caatacccct ccgtcaacat tcaaaaatac ataagtaatc gaaacaatga
    12061 ttacccaaga catgaagtga gggatgtaaa tgatcgtttg aatcccgttt ttcaatcgtt
    12121 tgttcgttac ttcatttaat agtaaagaca aaatgattgg catcggaaaa aacacgaaga
    12181 tatttaatcc gaacaagatc aacgtatttc ttaataatgt aaagaatgtc ggttctgtaa
    12241 acaaacgaat gaaatgtttg aaaccgaccc aaggacttcc cattattcct aaaaatggtt
    12301 gataatcttg aaaagcaata acgagtcccc ccataggaag atacctaaaa atcaagaaat
    12361 aaaggactcc cggcagaatc atcacatata aaaatttatt cgttttgatt cctgcaatta
    12421 ttttttttcg tcttctgagc ttttcttctt ttttcgatag cgtcagttga aatggttctt
    12481 gtttctttac actttccata agcccctcct tagttttgtg aatgtttgct gcttatgact
    12541 tcatgataga taactatctg aattacgtat ataatcattt ttatcgtaag taaaaacgct
    12601 tacatagcaa tgatttttgt gctatacaaa acgataaagt gtattttttc acaaataaac
    12661 tacgcatagc aaaccttcta tttttttcta tctgacactt agcataggaa atgattatat
    12721 acgtaatctt tcaccgatga taaattgaac atatagaaac agaaacattt aatttatttt
    12781 gaggaggagc ctatctatat gaaaaagaaa gtctctatag gagaaagaag ttttacactt
    12841 ttcaacaata cgtttctaat tttgctagct ttgatctgta tcgttccttt tttaaatatc
    12901 atcgctacat cttttgcttc tacacaagaa gtcgtcgcga aaaagtttat cctttttcct
    12961 actacctttt ctttagacgc gtatcgctac attctttcta cgccgaccat ttttagagca
    13021 cttgctgttt cgattggtgt tacaggtgtc ggaacgatcg tcagtatgtg tgcaacttca
    13081 ctgatggcct acggtctttc cagaaagtat ttgtttggtc gaggtttcgt caactttctt
    13141 gttgtatttt ctatgttgtt cagcgggggg atgattccaa cgttcttagt tgttcgttct
    13201 ctagggctgg tcaattctta ctgggccatg atcctgcctg tggctgttaa tgcaatgaat
    13261 atgatcatta tgagaaactt tttccaagcc ttgccggata gtttggaaga atcagcaaaa
    13321 atggatggtt gcacagattt tggtgtattt ttcaaaatta tgctgccatt agctttaccg
    13381 tctattgcta ctatctcgct tttttatgct gttacttact ggaacactta tatgactgct
    13441 attttgtata tcaacgattc atctaaatgg ccgatacaga ttcttttacg ccaaatcgtc
    13501 attgtatcaa gtgggatgca agcagaaagt agtgccgttg atgtgattcc accagcacaa
    13561 acagttaaaa tggctgttat cgttattgca acagtgccta tgctgattgt ttatcctttt
    13621 gttcagaaat attttgtcaa aggagcactc gtaggttctg tcaaagggtg attatgtact
    13681 gaacgcaaaa tggtagttgt gtcagaagtg gtcagcttca agaaataaga aggcatttac
    13741 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tttcgaagaa tctactcctg
    13801 gaacaccgtg tatcagaggt tgtgaaagag acgttcagcc ttgagcgatg ataaaaactc
    13861 tcacaaacat tgcgacgagt aacccacagg agcaataaga agcgattttg aaaaatagct
    13921 tcccatattt cacaaaatta tgccttattg tcgatagcct gtctcttgaa cgccatttat
    13981 tcgtaaaaaa gaactgtgac aagacttttg tcacagttct ttttgcgtgc ttagccttga
    14041 aatttaacta tccataaata caaatactag ggttattcta acttctttta ttgcttgaat
    14101 cagtccacaa gaaaaacagc ctcttgatta aacggcgttt atcttgttga ttctgcggca
    14161 ataaaatcag ccagcagcaa aacatgaagt gctgtttttt gctgtcctct tttattgctt
    14221 gaatcagatc acaagaaaaa cagcctcttg attcacaaat atttttttcg atattctccc
    14281 ggcgtaatcg attccttctt tttaaagaaa cgaataaaat tttgcggatt tcgatattgc
    14341 aatctttctg caatctcttt tacggagagg ttagtctctt tcaaccattt ttttgctacc
    14401 tctaaacgat gattttgaac aaaatcacca aatttttctc ctgtttcctt tttaaataca
    14461 ttacttaaat agttagggtt ataatgcaac ctatcagcga tcacatctaa agacagatca
    14521 cgatcatatt ctgtgtagac gatatgcata atattctccg acaaattttt aaattcatga
    14581 tcagtgattg cttgagagca ttctgtgatt ggcagaacta attgttctaa aacaagtttt
    14641 tctagttctt tgggatgata attgttcagt gcttttacat aaatttgttt catgttatca
    14701 aagttttttg aatctactcc caataactgt cctaattgaa cgagttcatt gatcaagcgg
    14761 accgaaacta cttcccgact caaaggattt ttattttgtg caaacaatgc atcaatcaat
    14821 tcatgtacta aattcttcac ttcctgattt ccagaacgaa tggcttcaaa caatcgattt
    14881 tgttcttcta tcggatatct aataagtgtt ttttcatgag cagcaggaac gatttcctga
    14941 taaaaaataa ttgaacttgg accagtattt acacgataat acagggcttc ttttgcccga
    15001 tcaaccgatt gcttgctttc attcagcgtt tcgaaccggt tactgatgcc tatactgata
    15061 gttaaattca agtattcttt tacacgtttt tgaatgagtc ggcaatactc catgactttt
    15121 ttgcttgctt ctgcatcatc tttatccatt cgataaatag tggcttgcat ttcttcattc
    15181 aaaacgatcg gcagcattcg attttccttt ggcacaattt ccgtgatcat gttgttgatc
    15241 gctaaaagta gcaaatcacg ctccccaccg tgactatctt ctaacagatc gatttgaacc
    15301 aaagctgtat aatagcagtc attttgagaa ctataaccaa attgctgcaa ccgctgatct
    15361 agttctcgcc tttctactcg atttctaaat aaactaagta caaatagcgt ttccagctgt
    15421 ggcttttggg taactaagtg tgcacttaac gcttcttttt ctcctatgat ccgactgact
    15481 gattgagcga ccaaatctag ttctttccca ctgaatcctt ttgatttaag attcagacgt
    15541 tcctgtattt gagaaatcgg acgggcaaat ctgtccgaaa ataagtagga aaaaagacca
    15601 atgagtccta ccagtgttaa agacactgca actagcccga ttctcagatt gcggatcatt
    15661 gcgccaattg cactagagtc aacttctaat ctgtaaatcc aattattata atctgatttc
    15721 agtactatat acgttttatc tttttctctt atcactttca gctgattgta atctagatcc
    15781 acgtccgtta atacagttgg ttgctcttta ttttttactg tctgagtagt aaaaaggttg
    15841 ccaacttcgt tttcaataga aagtatgtct tctccacttt caccaacaac ttgttctatt
    15901 gtatgtttct ctatcgtagc aattcccaat gcatattttt ctttttcaaa cattggcaaa
    15961 gaaatcacca tttcgattcc tttgtttttc ttttgccaaa ataaattatt tttgttattt
    16021 atgtacttct gttgatactt ttcaacttcc tcttcggtca gttgactcaa cgatccattg
    16081 attactcccc atttaccagc caaactgact aaatcataat tattatcatc cataacaatc
    16141 gtttctataa aatttagttc tttcctgatt tcagaataag tggagtaatc tgtatatgtc
    16201 agcggatgct tgaatatgtc cgagaaattt ttggtcgaac catacgtaga aaacgaatat
    16261 tcaatcgttc taattttcca ttcaacgtta gttttaactt gcctgagcaa attctttttg
    16321 tctgttaaat aactcgattc gatcgattca taggtattct ggtaaatgaa taaactaaat
    16381 cctatgacaa gtacggctcc taataaaaac atcggtaaaa atattttata aaatactgta
    16441 cttttcttca ttttcttcct ctcctccttt taaaataaaa ggctttcatt tttctgtaaa
    16501 attgatactc atctaattaa atgagcattc cccgctttca agaatagcat aattaaaaat
    16561 aaaaatccaa gtaattttta acgtgtgcaa tcttttcata tcaaagatct attgacttat
    16621 cgtagttatc gtaatatgat gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg
    16681 gaatggtaac gatcaaagat atcgctaaag cagctggcgt ttctcatacg acagtttctc
    16741 gtgctttgaa taacagcagt ttaataaaaa atgaaacaaa acaaaaaata cgacaactag
    16801 ctaaggaaat gaattattcg ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat
    16861 atctaatcgg actatttttc tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag
    16921 attccattgc aggcatccat tcagtattag acacaaatta cagtttatct gttgaaagta
    16981 tcgatgttgt tgatttaaca gaaatcaacc tgcaacgcta cgatggactg atcgttatga
    17041 gtcaaagtga tgatgatcag cctttcatcg actatctcaa agaaaaagaa tttcctttcg
    17101 ttgttgtcaa tcgtcacatc aatgatccag cggttatcaa cgtcactgcc gatgatgcgg
    17161 ccggtgttat gcaggcagta gattacggta ttggatgcgg acatacgaag atcgcttata
    17221 ttggcggaaa gaaaaatttt cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc
    17281 taaaaaaagc gagcatccct gtcaatctag cctatttttt aacaggagat tacagcttag
    17341 aaagcggcct ccaaaacatg gaatacctat tatcactttc tgacctgcct actctggttt
    17401 tttgcggcaa cgacgacata gcgattggtg cattgagagc tgtacattta gctggcctcc
    17461 gtgttcccca gaacatttct ctgatcgggt tcgatgattc tccagtcgtc tcctatctca
    17521 atccgccgct taccaccgta cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc
    17581 ttttactaga tttgatcgaa ggcaggacga tcgaacaatc taaaattgag ctctccacct
    17641 tgttgatcct tcgtgagtcg atcaattact tgtaaactac aagtaatttt atataaaaat
    17701 gttaacgtgt gcataacaca gaaaggaagt ttatatgaaa aaactgacaa aagaattagt
    17761 gaaaaaaaac gaagcacctg taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg
    17821 atttattgat tggcagattc agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc
    17881 aatcattcaa cctttagaac aaggtcttgg aaatatgatg aaccagcaag ataatttata
    17941 tacggtcatt ttacaaggac tcagcaatgg cgaaatcgtt gatacttccg agattatcac
    18001 cgtagtagaa caaatgatca atccttatga tgaatgggat gcttatctat ctctagcaga
    18061 aaatgacgac ttggcctata tcgtctccaa tacaacagag gcaggaatcc aattcgtgga
    18121 gactgataat ttaacagata ctcccccaca gagcttcccg ggcaaactga cagccttttt
    18181 ataccgacgt ttcaaattaa acaaagctgg ctttaccatc attccttgcg aactgattga
    18241 cagaaacggc gaacgtctaa aagaaatcat tttacgctac gcagaaaaat gggatttaga
    18301 acctgctttt gttgagtggt tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg
    18361 catcgtccca ggttatcctc gcgaagatgc aggtgttttc aatcaacggc ataactatga
    18421 agatcaattg atggtcaaag cagaaccatt catgctttgg gtgatcgaag ggcctaaaga
    18481 attggagaat caactaccat tcaaacaggc tggcttgaat gtcatcatca cagatgatat
    18541 gactccttat cgtgaacgta aggtgcattt gcttaatgga ccgcatacag cgatggtccc
    18601 tcttgctttg ttggcggaat tggaaactgt agaagaagta atgaaagatc ctttattctc
    18661 cgcatatgtt gatcagttat tcaatctaga attgattcct atgctttctt tacctaaaga
    18721 tgaattagcc atttatgctg accaaatcaa agaacgtttc ttgaatcctt ttgcccatca
    18781 caaattagaa gcgatttcat taaatagtgt ttcaaagttc agcacccgtc tgcttccagt
    18841 gttcaagaag tatatcgaag aacaaaatca ggtaccaccg ttaatcactg tttcactagc
    18901 tgcgctactt cttatgtatc gaggcgatca agtaaagcca catgatgatg aaaagacgat
    18961 aagcgaattt actgatgctt ggtctgataa tggaacagct attcctcgat tattgcaaaa
    19021 cgctgcgctt tggggagaag atttgtctca aataccgaat gtgacagata cagttcaaga
    19081 aatagctgca aagatcgatc aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac
    19141 gatggtccat gcttgaaacc ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg
    19201 tcgtcgcatt aacccctatt ccaaaacaaa cacaactgac gattgatcag caaacgatca
    19261 ccactcttga agatatcccg caaggtcata agattgcatt gggtgatttg aaagctggag
    19321 acaacgtgat caaatatggc tatccaatcg gtcatgtgac gacagacgta actgccggtc
    19381 aatggctgca cactcataat gtaaaaacca atctttctgg tgaattggat tatcgatatg
    19441 aaaaagatgt ccatccaagt cattaccttt ttgagaaccg aacatttcaa ggttacttgc
    19501 gaaaaaatgg aaaagtaggg attcgtaatg atttgttttt agtacctacc gttgggtgtg
    19561 tgaatggcat tgctgaactg atcgtcaaac agttcaagga aaaacatcca gatctgggac
    19621 agtttgacca tattacgatt ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc
    19681 atcaaaatac tcgagaaatt ttagcggacg cagtaaatca tcccaatgcc ggaggagttt
    19741 tagtatttgg attaggttgt gagaacaata cagttcctga attcaaaaaa attttaggtg
    19801 aatatgatga agaacgagtg aaatttctag tggctcaaga tgtttacgat gagatcgaac
    19861 aaggtgtggc tctattagaa gagcttttaa cagcggcgga aaatgaccag cgcatatcag
    19921 tcccgctctc taagttgaat gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa
    19981 taactgctaa tcctttactt ggtgcatttt ctgactactt gattgctcaa ggaggcagca
    20041 cgatcttaac agaagtgcca gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg
    20101 aaaatgaaga agtttttgac tcaatcgttg acttgatcaa tgacttcaaa cagtatttct
    20161 tgtcttacgg ggaaccagtt tacgaaaacc catctcctgg taataaagca ggtgggatca
    20221 ctacattaga agataagtct ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg
    20281 atgtgctaca atatggcgaa aaaatcagaa aaccagggct tagtttattg caagctcccg
    20341 ggaatgatct tgtagctgcc tctgctcttg cttcatccga ttgccagttg gtcttgttta
    20401 ccacaggaag agggactccg tttggctcat atgtcccaac ggtcaaggta tctacgaata
    20461 caactctttt tgaacgcaaa ggtcattgga tggattttaa tgcaggcgtc ttattagaac
    20521 aaccaatgga agtaatctta gaatcgtttg ttcaaaaaat catcgctgta gctagtggag
    20581 aagaaacgaa aaatgaacaa aatgacgttc gagaaattgc catttttaaa aacggagtaa
    20641 cactttgatt ttcataaaac agtataaaaa gaaagaagga agatttatgt ttttatctga
    20701 tgatttttta ttaaaagacg attgggcgaa aaagctttac cattcctacg caaaaaacat
    20761 gcccattatt gattatcact gtcacttaga tccaaaagaa atttacgaaa acaacaattt
    20821 cagcaatctg acagaagctt ggttaggagg cgatcattac aaatggcgct tgatgcgagc
    20881 ttgcggcgtg cctgaagaaa aaatcacagg aaatgcttct gattttgaaa aatttttagc
    20941 ttggtgtcaa accgtaccaa aaattatcgg aaatccgctt tacagttggt cacatcttga
    21001 attgaagcgt ttcttcggta ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg
    21061 gacacaagca aatgaaattt tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa
    21121 atcgaatgta gaagtgattt gtacaacaga tgatcccgca gacgacttgc actatcatca
    21181 gttgcttgct catgaagaac cagatttgaa ggttctacct tcattccgtc cagataaagc
    21241 actcaatatt gaaaaagaag gatttaatgc atgggtcaca ctactggaag aagcagctga
    21301 ccaaacgatc accacctatc aagaactgat agaagtctta gggcagcgga tagaatactt
    21361 ccatcaacat ggatgtcgtg tttccgacca cgctttagac atcttacgct ataaagaagc
    21421 ggatgaaaca gtattagaag agatttttca aaaagcacga gctaacaaac tattatcttc
    21481 agatgaaatc gatgcttatc ttacagaaac actgatacat ttgacccact tctatcattc
    21541 tcataattgg acgatgcagc tgcacatcca tgcttatcgt aactgtaata cacaaatgtt
    21601 ggaaaaatta gggcccgata ccggctatga tggaatgaat gatctttctt tgactattcc
    21661 tctgcaaaaa ttattgaacc gtgcagaaga aacggatcag ttgccaaaga cgatccttta
    21721 ttcattgaat ccgaatgact atcctgcgat tttagctttg atgggctgtt tccaaaaaga
    21781 acagaatgga aaactgcagt taggttctgg atggtggtac aacgatactc gagctggtat
    21841 gcgcgatcaa ttgacacaat ttgctgatgg tagtgctttg gggaattttg tcggcatgct
    21901 gacagattca agaagtttcc tgtcctacac tagacatgaa tatttccgtc gtgttctttg
    21961 tgaattttta ggcgaaatgg tcatgcgagg agaagcacct gaagatgaac aacttttagg
    22021 aagtctagtt caagcgatta gttatacaaa tgctaaaaat tactttggct tttttgccta
    22081 attgattaat agtaacagta acgaatcaaa aacaggatag tgaaataacg gaatccgttt
    22141 atccacgttt tagagaagaa gacggaccaa attt
//
LOCUS       DACDSR010000040        22033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDSR010000040 DACDSR010000000
VERSION     DACDSR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.040"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            369..557
                     /locus_tag="IWK15_001537"
     CDS             369..557
                     /locus_tag="IWK15_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547131.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            547..807
                     /locus_tag="IWK15_001538"
     CDS             547..807
                     /locus_tag="IWK15_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547132.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            848..1075
                     /locus_tag="IWK15_001539"
     CDS             848..1075
                     /locus_tag="IWK15_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547133.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1140..1712
                     /locus_tag="IWK15_001540"
     CDS             1140..1712
                     /locus_tag="IWK15_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547134.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1713..2024
                     /locus_tag="IWK15_001541"
     CDS             1713..2024
                     /locus_tag="IWK15_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9547135.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2015..2275
                     /locus_tag="IWK15_001542"
     CDS             2015..2275
                     /locus_tag="IWK15_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547136.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2436..2795
                     /locus_tag="IWK15_001543"
     CDS             2436..2795
                     /locus_tag="IWK15_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547137.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2792..4435
                     /locus_tag="IWK15_001544"
     CDS             2792..4435
                     /locus_tag="IWK15_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9547138.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4470..5552
                     /locus_tag="IWK15_001545"
     CDS             4470..5552
                     /locus_tag="IWK15_001545"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547139.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5596..6768
                     /locus_tag="IWK15_001546"
     CDS             5596..6768
                     /locus_tag="IWK15_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9547140.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6755..7459
                     /locus_tag="IWK15_001547"
     CDS             6755..7459
                     /locus_tag="IWK15_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9547141.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7464..8624
                     /locus_tag="IWK15_001548"
     CDS             7464..8624
                     /locus_tag="IWK15_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9547142.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8700..8978
                     /locus_tag="IWK15_001549"
     CDS             8700..8978
                     /locus_tag="IWK15_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9547143.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8959..9330
                     /locus_tag="IWK15_001550"
     CDS             8959..9330
                     /locus_tag="IWK15_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9547144.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9346..9750
                     /locus_tag="IWK15_001551"
     CDS             9346..9750
                     /locus_tag="IWK15_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547145.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9747..10157
                     /locus_tag="IWK15_001552"
     CDS             9747..10157
                     /locus_tag="IWK15_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547146.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10218..10805
                     /locus_tag="IWK15_001553"
     CDS             10218..10805
                     /locus_tag="IWK15_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9547147.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10878..11345
                     /locus_tag="IWK15_001554"
     CDS             10878..11345
                     /locus_tag="IWK15_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9547148.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11391..11699
                     /locus_tag="IWK15_001555"
     CDS             11391..11699
                     /locus_tag="IWK15_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547149.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11894..18628
                     /locus_tag="IWK15_001556"
     CDS             11894..18628
                     /locus_tag="IWK15_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9547150.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18632..19504
                     /locus_tag="IWK15_001557"
     CDS             18632..19504
                     /locus_tag="IWK15_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547151.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19501..20128
                     /locus_tag="IWK15_001558"
                     /pseudo
     CDS             19501..20128
                     /locus_tag="IWK15_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20089..20550
                     /locus_tag="IWK15_001559"
     CDS             20089..20550
                     /locus_tag="IWK15_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547152.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20554..21378
                     /locus_tag="IWK15_001560"
     CDS             20554..21378
                     /locus_tag="IWK15_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547153.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21396..21941
                     /locus_tag="IWK15_001561"
                     /pseudo
     CDS             21396..21941
                     /locus_tag="IWK15_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7878 a         3298 c         4671 g         6186 t
ORIGIN      
        1 tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat tatgtcactg
       61 tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt tcgattcctt
      121 gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
      181 tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta gattgagttt
      241 tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa acgtccctgc
      301 ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa aagagaggat
      361 tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat ttttatgaag
      421 tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg aaggctacgg
      481 gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg cttgcggagg
      541 tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg tgaaagaatt
      601 gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt catagtctgg
      661 tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa aggagcaccg
      721 tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac ttatctcaaa
      781 aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac ataaaggagg
      841 ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc gaaccattgg
      901 tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga
      961 aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat attgcggtca
     1021 tggctattgg aaagacgagt atatccaata caacattgag aggtacatca aatgaatgat
     1081 aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg aggaaaacta
     1141 tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc aatctagctt
     1201 acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct ttagatgcca
     1261 ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct gttcttagtg
     1321 ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt actggatatg
     1381 agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag atcaaaaaga
     1441 ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa atcaaactaa
     1501 gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat gagatgccga
     1561 taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc caagtaccaa
     1621 aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag tacttagata
     1681 gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt aaacaagcaa
     1741 gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc ttagagcgag
     1801 atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa catgattcaa
     1861 ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct ctagatataa
     1921 acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt atgaaatatc
     1981 gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa cagcaaacag
     2041 agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg ataagttggt
     2101 tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat ctatcaacaa
     2161 tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg tagacattga
     2221 ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg gctgataaat
     2281 cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt tgagaagaaa
     2341 taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg ctattttcac
     2401 acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa gagagaaata
     2461 ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt gataaggata
     2521 aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt cttcttgatg
     2581 aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag tcagaaaatc
     2641 aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga gaccaagcat
     2701 atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc gaacctacaa
     2761 aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat tctattttga
     2821 tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa gaattcagtt
     2881 aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg atgaagaaat
     2941 gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta aatatcaaaa
     3001 atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt tttttgatga
     3061 aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag caagagactc
     3121 attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta cagcgaattc
     3181 tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa tgcgcaaact
     3241 ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta attcgatttt
     3301 tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct gcttagaatt
     3361 tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta gtgggctagg
     3421 gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta gagaaggttt
     3481 ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca agaatgatcg
     3541 cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc ctaaaatgtg
     3601 gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc tgttttctac
     3661 agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg aatttgttgt
     3721 taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc atgaaaaatt
     3781 aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg ctggatttga
     3841 ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg atgataaatt
     3901 tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca aactgaaagc
     3961 gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc catcaattga
     4021 tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa tagaaatcgt
     4081 ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag aatcatataa
     4141 ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta ttattgaaga
     4201 tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt ggtatacaaa
     4261 taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa aagagccagt
     4321 aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac gagaagaaat
     4381 aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat tttaaggtat
     4441 actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta tttgttacaa
     4501 tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt actgagtatt
     4561 taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta agtaggctag
     4621 aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg gtatttgtga
     4681 atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa gtaaagcaag
     4741 ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat cgtgagttct
     4801 attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa caagctgtta
     4861 ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac tatgcattta
     4921 cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca ggaccgctga
     4981 agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg atggggaatt
     5041 atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta gctatattga
     5101 atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg tttcaccgaa
     5161 tagttaaaac attaaatgag tattatgacg attataatga ttttgatgtg aagaggtata
     5221 caaaatatat agggacatta agaacacaaa tttctccagc tgaattccaa gtgattttat
     5281 tcaattctct atatataaaa cgaggttttg gcttaggaat tcaacttatt ggaagtggtt
     5341 tttttggaga tgattttgat tttgaaacta atcaacattt tgaaacaagt atcaatgaac
     5401 agtggtttct atcactatca actgttgaca gtgataactc tataaagcga caaaaattat
     5461 ctgaatatat taaagaatta ggatccgtag aatataaaaa aatagctaat tttgaaagtt
     5521 tatataaact ctttaatgaa actaagctct agaaataggg cttttttatt tttctttgaa
     5581 aggtggtgaa aacatgtgag cttatttgat atctttaagc agtcaataag aaatgaagag
     5641 ccttctgagt ggataccgga ttttgtatat ggagaggaag tatctgctcg ggcatacctc
     5701 aaaattatgg caaaaaatac tgttttagat tttgttgcaa gaactatgtc aacgttagaa
     5761 gttaagttta aaaataaaga tgggacatca gactgggatt atattctaaa tgttcgacct
     5821 aattctgata tgtcagcagc tacattttgg caaaaatttt tttatcggct cttagatgac
     5881 aacgaagtgt tagtaatatt tacagatgat aatcaattgc tgattgctga tgattttagt
     5941 cgaacggagt atgccgtgta tgatgatgtt tttacaaatg tgatggtaaa aaattttgtc
     6001 tttgaacgtt catggaacat gtctgacgtc atttatatgg aatataataa cgaagaatta
     6061 gaaaggttta cacgaggttt atttgaagat tacgccgcgt tgttcggacg gattctagaa
     6121 gttgcaatgc gtaacaacca aattcgtggg tctgtatcca ttgattcaac gggaacggcg
     6181 aatgatgaaa aaggaaaaga tggtaagact agatctcaaa gattacaaga atatattgat
     6241 aggatttgga actcttttaa aactaactca gttgcaattg ttcccaaagt gaaaggcttt
     6301 gagtatgagg aatacaccaa taaacaaggc gtatcgaatc aatcgctaga tgaactgaat
     6361 aagatgaagt cgtctttaat tgatgatgtt gccaacgcca taggagtacc tacggcgctt
     6421 atttatggtg aaaaagctga actggattca aatattaaag cttttcgtaa attgtgtatt
     6481 gctccactag ttaaaaaact tcaagacgaa ttggtggcaa aaataattag tcgtcaagag
     6541 tacaaaaacg gggaacgtat agaagttaaa aaagttctcc aaaaagatct attagagtat
     6601 gctactcaaa ttgataaaat aacatctggt ggtgcgtttt atattgacga agttcgtgat
     6661 gaagttgatt atgatgagtt gccaaataat gaaggaaaaa ttcgtattcg tacgaaaaac
     6721 tatgaagaaa ctgtgaaggg aggtgagaac gacaatgccg aaagctaaaa aagtaccatt
     6781 tcagtttact aacgaaattc agaatggtaa gcacattctc accttgagtg gaaatgtaca
     6841 gaaaaaatat tggcgtgatg atgatgtcat aaacgctaaa gatattcgtg actctctaga
     6901 tactgtaacc gatgatattg taatcaagtt gaacagccca ggcggtgatg tgtttgaagg
     6961 gattgagatt tacaattatt taaaagatca cccatcaaat attactgtag aagttacagg
     7021 gcttgccgca agcgcagcaa cgtttattgt cgctggtgcc aatgatgtga ttatgaatgt
     7081 tgggacttct ttgatgattc atgaagcttc tacatttgct tgggggaata aacaggacat
     7141 ccaaaaaacg ttaaacgcat tggaaacgat cgatgattct atcttagcta tttattcaga
     7201 taagactggc caatctgttg ataagttgcg tgaatggatg aatgaagaaa aatggtttac
     7261 agcagatgaa gctgtcaaat atggatttgc caattctgtg aagcgtgaag aaccacaaaa
     7321 tgaacctcaa aatattgcag cgatgattca agatgctgtt gccgttgcaa tagcgaattt
     7381 aaaggaacct gttactaata aaatcgaaca agaatcaaaa caaaaatcat taatcgcacg
     7441 attgcgaaaa ggagaataaa gatatgttaa aaattacaga taaaactgca gatgcaaaaa
     7501 aggcatttaa tgctatttct tcgaaagaag atgcaacacc cgaacaaatt aatgctgctc
     7561 tagaagaata tgtaaccgcc attgcagaag atgccggcaa acaggtgcgt gctgaatatg
     7621 aagaattgaa aaatgtgacg gacaaccgtg tattagaggc tcgtggaatt cctacactaa
     7681 cagcagaaga aacaaagttt tataatgaag ccgtaaaatc tggaggtttc gattctgacg
     7741 tagtttggcc agaaacgatt ttggaacgtg tttttgaaaa tcttcaaaac gatcatccta
     7801 ttttaggcct cattaacttt acaccaactg taggtcgtgt caaagtaatt agagcacgtc
     7861 gtaaaggtgt tgctgttttt ggaccattgc ataaagacct tgaaggccaa ctagatgctg
     7921 aatttggtgc aactgaattt gttcaacttg cgttgaccgc atttttctta atttctaatg
     7981 ataccttaga tttaggacca cgatggattg accgttttat caatctttct ttaagtgaag
     8041 cagtacgtga tgtttgggct aaaaaaatta ttacaggttc tggtaaagat gagccaattg
     8101 gattgcttaa agatctagat ggtgcggtca caagtggtgt atatccagat aaagctagcg
     8161 cagggacatt aacttttgcg aaagagaaga ttgttcttga acttgcaggg gtgatgaaaa
     8221 aattatctaa atacacatac aaagtagatg gtgacgatga aggtgaagtg aaataccgtt
     8281 ctgttgctgg acgagtgtat ctaattgtta atcctgttga ttactgggat atcgtagcta
     8341 gagttacatt tgcaaattta aacaatgtgt atgggactac gatgccattt atcgctgttg
     8401 atcatatcat cgaatctgta gacgtaccag aaagtaaatg tattgcatac gttgataaag
     8461 agtatgaagc tactcaatca agagctgaaa aagtctatgt atataaagaa acttttgcaa
     8521 tgaaacgcgc aactttgtat gctgtcgaca tgcttggtaa tggctatcca acaaataatg
     8581 atgcagcaca agtatatgac ttggacttta cagcggggga gtaatgtccc ctgcaattgg
     8641 tgagataaca ccgacgactg atggggcagt cattaatctt tcgtaaatgg ggttagagta
     8701 tgaacactga agaatttata aaagaatata aggcccgatt tcgtattttc catacttccg
     8761 aagatgagga tatcgggaaa cagttagaga gcgggtttgc cgatataaaa tctctaatcg
     8821 gcagttttga tccttccact tatgagaaag gaaaagaatt agtttatgaa cgtactcggt
     8881 atctacggaa cgaatcacta gagtactttt acgacaattt tcaaacaatg attatggatg
     8941 catcaattga tctggcaggt gctggatatg ccaattaaat caaattataa gaagcctaaa
     9001 attgtagctg gtgatctaaa tacgccagtt actttttttg agtttcaacc taataagggt
     9061 ccagaacctg gtgatgaaga aaaaaaggaa ctgtattttt gtacagcact agtttacaac
     9121 ccatcaatga aagatcgtga cattcttggc acaaaagaag gcgttacgat aaaaatacgg
     9181 gatccccatc aatcttatat tcctactaac aaacataaag taaggatatt agattatcga
     9241 tatggagata aagtttggga aataatcgat gtctcaccgg atttggaaga aaattcaatt
     9301 gtaaagatag tattagaatt aattacttaa atttggtgat aaatgatgag taacgtaaag
     9361 tttgaaggcg ttcaagaaac aatccaagct ttagaaaaaa agtatggaga aaagaaaact
     9421 aagacattaa caaaaaaagc aatcaatgtt ggcgctgaaa aagtagaaaa acagttgcaa
     9481 acggacatga ccgtttttaa agacaaaggt tatacgatcg ctgaggttgt tcgcaagaac
     9541 gctacgtata gagattacaa ggcagaagct gaaatcggtt ggaatggtcc acatcaacga
     9601 tatagaatca ttcatttgaa tgaatgggga tatacacgaa agggcaaaca aattagaccg
     9661 agaggatttg gtgtaataac aaaatctctt aagaattccg agctgttgta ctttgatgct
     9721 gtaggagagg aggttaaaaa gagtttatga aagatatgct taatatcatt tacgaggctc
     9781 tttgtttgaa tgagtatatt cattcaatga cctacaacga gaataccgag caatatcgta
     9841 ttaaatttta tgagcaacct gaaaccgccg ataaaacagg tccaatgatc acaattcgtc
     9901 ctgtagatac acctaacgaa gcgtaccacg gaagtaacaa agagctttct gtggagcata
     9961 tgatacagat tgacgttgaa tcgaaatata gagcaacatg caagcagatt caatatgaga
    10021 taaaaaaaga gatgaaaaaa ctaggctttg gccaaatttc cggtcaaggg ttagatgaat
    10081 actttactga aacaaaccgc tttgtagacg cgcgtcgtta cgaaggaaat acaaaaattt
    10141 acgataccga atattagaaa ttaagacacg aaaataacgt gtcttttttt attacccaaa
    10201 attaggagga aaattttatg actttagtag gattcaaaaa aatgacaatc ggggtctttg
    10261 acagcacagg gaagattcca gcagcaaatt tatatgtaat tgaaggtgaa caaaacaaag
    10321 gggcgactgt atctgctgaa atcagtgggc tatcaaaaga accatcaaaa gtttacggat
    10381 cgaacatcgc ttactatgtc tctcaaaaag gaacaggcga tgtttcagct acttttggat
    10441 tgttagattt accaacagaa gtaaatgaca aaattcttgg ttacaaaacc gatacaaaca
    10501 aaatcagctt cttgggagaa gatactgagc caccatattg tgcaattttg atggaatcgg
    10561 aggatttgaa cggcgataca gcaatgcttg cgatgttcaa aggaaaattc agtcgtgaat
    10621 ctattaatct taacactacg acaaatgaag ctttcgaacc agaagcagaa gaatatgtat
    10681 tttcagcgat tgccaatgat gttgaaggtg atgctaaagg ccaaacagtc gcaaaatatg
    10741 ttggagacga agaagcttct attactggtt tgaaagagat ggtatttcct gcagcggggg
    10801 agtaactagc cccgttgttg gagcaatcac tcccactatt aatggggcaa cagttgaatt
    10861 aagttaggag gataactatg ccagatacat ttaaaatcta taaaaaaaat ggtactgaat
    10921 ttacaaaagt cgcagagggt gaaagtccac tttctattac tggaattgca gcaaatactc
    10981 aagttgctaa aggagattat caagctacgc gtgtggtcgg aggtcaagaa tcagtaaaag
    11041 tagatattcc tgcattcacc actcttccaa tttcagttac tggtgtgacg ttggataaaa
    11101 cgacagctga agttgaacaa ggcggaacct taaaattaac tcctactgta acaccagcaa
    11161 atgcaacaga caaaacagga agctggagca gttcgaatac agctgtagct acggttactg
    11221 gaggcaacgt tacagtaaaa acagacgctg aagttggcgg aactacagaa gtttcattta
    11281 ctactacgga tggcggtaaa gtagcaaaat gtactattac agttattgaa aaagcagcgg
    11341 gctagtcttc ggactagctt ttttatttgg aaaataatgg aggaatctaa atggcaaaag
    11401 taagaattga attgaaagaa gaatcaggaa agaaagtcta tgaaaacata gacacaacag
    11461 ggaaagatta tcgaaaagct ttagaaacaa tcaaaaagct aaatgaagac ggagtaatga
    11521 tttggaatcg tttagacatt tatttggatt ttgctgtagg tatcttcagg gatagcaaat
    11581 tgacagctga acaaattctt gatggcttac catctgagaa ggtaatgagc acattggacg
    11641 acatcctagg agaagtaatg gggattgaaa gtaatccaga cccagacgca aaaaagtaac
    11701 acccgaagaa gcagaagaaa tgtacttaaa cctatgcaga gagttagtga aagcgggatg
    11761 gtcactatct gacatagaag ataattcctt tgaaatgatg atgaaaattg cgtgcacttc
    11821 gcctaagaaa gagaaacaaa aagaagttga tcttaaagac ttcttgaaat ccatgtagag
    11881 aggaggtaaa catatggcaa atggaaaacc tttaggcaat atgattatca aactagactt
    11941 agacagttca gctttttcaa aaggtctagc cggagctaaa aacgctgtta accatcaaat
    12001 gaaagcaatg aaatcacaga tgcaagtaat gaactcttca ggaaattctc tcggtgcttt
    12061 acaagcaaaa tacaatggtt taggcggagt tctacaagca aatgaaaagc aagtagaatt
    12121 gctcactaag gcttataagg atagttttga ctctaacgga aacgctactg cttcgactgc
    12181 taaatacgct aatcaattga atcaagcaac agctagatca gcaagttacg aatctcagat
    12241 gaaaacaact gttggacaga ttgctcggat gaaagtggaa acggaagggt ttacggggaa
    12301 actaaaggct aattctgatg tattgattaa ttctgggaaa aaaattgaag cctttggtaa
    12361 aggagtttct gggataggat ctgccctaac tgttggagta acagctccaa tagtggcagg
    12421 cgcaactgct gttactagtg cagctatttc atgggaaagt gcttttgcag gtgtaaaaaa
    12481 gacaaatgat gaagttgtag actcgaatgg aaacgtagtt tattcctata gcgacttaga
    12541 gaaaggtctg cgtaatcttg ccacccaatt acctggtagc catcaagaaa tagcaaatgt
    12601 agccgaagca gccgggcaat taggtataaa gactgaaaac gtagttagct tcactaaaac
    12661 gatgatagac atgggtgaat cgactaatat gtcggctgaa actgcggcaa cttctttagc
    12721 acgatttgct aatattaccc aaatgtctca aaaagacttt gataaattag gatcagtcat
    12781 tgttgatcta ggtaacaatt ttgctacaac cgaatcagaa atcactgaaa tgggattgcg
    12841 gttagcggga gctggtaaac agattggaat gagccaaggc gaaattctag gatttgctac
    12901 tgctctatct tctgttggtg tcgaagctga agcgggtggg tctgcctttt cgaaggtcat
    12961 gatccagatg caactagctg tagaaaaagg atttggcgct tttgatcagc tgaaacagat
    13021 ggcagaagaa caaggtgttc cgtgggttaa tttagtaaat gctgtgagag acggtggaaa
    13081 atcgttaaaa gcagtatcag agcaaatggg attcacttca tctgatttaa agaaaatgta
    13141 taaagaagcg gacaattcga aaagttcgct tgaaaatttt gcgaatgtgg caggtgttac
    13201 aagtgaacaa ttccagaagt tgttcaagag tgatccttca gaagcaatca tcaagttcat
    13261 tcaagggctg aaagattcag aaaaacatgg tacttcagca atcaaagttc tggatgacat
    13321 ggatataaaa gaagttcgac tacgcgacag tttattgaga gctgcaaatg ctagtgacat
    13381 attcaaagga gcaattgaaa aaggaaacgg cgcgtggaaa caaaatacag ctttaacaaa
    13441 tgaagctaat aaaagatacg aaacaactga atcaaagcta aagatattga aaaacgaagt
    13501 gattgataca gcaatcgatc ttggaggtcc atttgtagac gcgttgcgtg atggtctaaa
    13561 tgtatcgaaa ccattgataa aaaacttagg gaatatggcg aaagctttct cagctatgag
    13621 tgatgatcaa caaagaaaca ttatcaaatg ggttggatta gcagcagcag ctggtccagc
    13681 gctgaagctt ttaggaactg gtacttcagt aataggtaaa gttacttcaa ctactggaag
    13741 attgacgaaa agccttgttg atttagcagc gaaagcagca gaaaagaagg ctatggatgc
    13801 ctttgcatcg tcggtaacaa cagctggtgc tgcagctgca aagacagcag gagctggagg
    13861 tattggcggg ttagtcccta tgatgggaaa aacagctagt gctgcaagta ctgctgcggg
    13921 cgccagcgga ataggcgcaa tgacaacgtc actaggtttg ttaggtcctg ctctacttgg
    13981 tattgttggc gttggtggcg cactggcagt tggctatgga gcatggaaat tatttggtga
    14041 agaagcttgg aattcctcgc aaagagtaaa gcaatgggga acagatgtcg gtgaggagat
    14101 agatagtact cttgacggag tacagaagaa tattgaaggt gcaaatggtc aattcggtct
    14161 tctcaaagag ggttttactg ttgatgatgc aaataatatg gcggctaatt tcgcaaccgc
    14221 tggaacatct ttggaaacat ctttaacggg tagaataaag gcattagacg atgcactaaa
    14281 agggctacct caatcagttc aggatgccat gaaagaagta gctgaatcag aagaagaaac
    14341 cttagggcaa tctctttcta aaataaaaga caacaatgaa gaaataaaaa aaatcagaga
    14401 aagagccgca aatcagaaca gagacttgac tgtttcagaa ctacaacaga tacaggatct
    14461 aatgaaagga acttctcaag cgtatgtgga tacgctcgat gtttctgcta aagacagaaa
    14521 aaagatacta tcagccatga atggtgatgt tgcacaagct acagaagaag aagcgaagac
    14581 gtggttacaa tctttagcga aacaaaaagc tgcaactacc gaaaattata gacaaatgct
    14641 tgaagaaaaa aaggcggctt taaaggaaaa gggagtaagc aaagaattca ttgatgcatt
    14701 agaagatgaa atgaatgatt acatcaaaac tacaaatcaa ggattcgata gccaaatagc
    14761 tgctataaca aataaatatc cacaattgat taacgaagtg agtttatcaa atggtcaatt
    14821 atttagcaaa acggcagctg ctatggacaa tactggtcag tatacaaaag tttttctcga
    14881 aagtaatgaa aagattttag cccaagcgaa gaaaacatcg gatgaaatag cagataatgc
    14941 cgaaaaaaat gcagaacaac ttggtatggt agctgatgag gctacgcatg ctggatcaac
    15001 ttggaatggc ttagttttag acgaaaaaac aggaaaagta aaaactaatg tacaagaagt
    15061 agtgaatgaa gcaacgaagg atttcactaa atggaatgaa ttgaaacctg ttgttcatga
    15121 tgcgaaacta acaacgaatg caaaagatgt catttccata gcggcagtac aaaatggcta
    15181 ttgggatgat atggaatgga aagaaaaaga agcattatta gaagatgagt gttctaaaaa
    15241 tgtatacaag gctttagaaa atgctggtaa atggaatgag ttagatattc cttctaaaga
    15301 agcaatccta acttcaaata ctcctgaagt tatgggtgaa actctcatga atttgggatt
    15361 atgggatact tacaaacttc aagtgaaaga tttagatttg aatgcgtata aagtatacga
    15421 tgctatcgat ggatcagaag aaaaattaaa tagttgggct caattagata accccacaaa
    15481 agaattgtta ttagataact cagatttttc tacaaaactt tttaattccg aagaaatgtt
    15541 taaacgttgg aatcagttat catctaatgc aaagtttctt tcggctaata atactaaatt
    15601 tacagaggtt cttttatcat ctgacagaat gtggaatcaa tgggtaaacc ttcctgataa
    15661 tgagaaaaga ttacttgcag ataacgaaga tttagctacc actgttttat cttcagaaga
    15721 aatatacgga agatggactc aattaccaga taatcaaaaa tatatgttag ctgacaatac
    15781 tgacttagca aacaaaatat tcgcttcaga agaatcatgg aatgcatgga aagcaattcc
    15841 agacacagtt aaaaacttgc ttggtaataa tattgattta ctggctaaag tgaaagacgg
    15901 gactgtaagt gtagaagaat ataacaaagt tatgccactt cttaaaactc tatatggaga
    15961 taacgttaat ttaaaagagg caatcagtgg ggctaaaagc tctatagaga attacaacaa
    16021 aaatattttt cctagtgata aaactgcagt aggacacgat gaggcttcac aagctgctaa
    16081 agatgcattt gaagcgttca acatcttcca aacaaaaata ccagataaaa ttactaaaac
    16141 tgtatcagct gactttatag gtcctatgcc gaaaaatgcg aaaggaacaa acttccatcc
    16201 tggcggagca gctatggtaa acgatcaaaa agggcctact tatgaagagc ttataacctt
    16261 gccaagcgga gaaactttta tacctaaagg acgtaatgtc gttttagact taccaagagg
    16321 ttcgaaagta ctgaacgcca ctaaaactaa acgtctagtg cctaaatatg ctgatggtat
    16381 aggaaacata acgacggttt catctacgct aaacttagat gctttaattt tagctattaa
    16441 cgagctaacg acggtattga gaactcaact gatggcagga acgactgatg ctaaagatac
    16501 taaactatca gatgaaacaa aagttgccga tccgattatt ccggacagtc tttctgaaaa
    16561 atcagatcag tatctaggaa ttggagctca atggttaact aatctaatga atggttggaa
    16621 ctcagcggtt cctcaatata tgaatagtga aatggttttt attacaaact atctcaatgc
    16681 gctaaaactt caaaacaatc ctaattatac gcaaggagca acatggaata agaacttact
    16741 gaatggatgg aatagtttaa ctgggacctt tattgctaca atcaattcat tttgtaatca
    16801 agcaatggtc acgcttagaa actacaacac tcctatgtac aacaatggcc gaacgtggca
    16861 gcaaaataat cttaatggtt ggaattcatt gtatggatca ttcatagccc gtgtaaatca
    16921 gcttggtaac gattcgatta acaaccttcg ttctaaaagt ggtggttttt acaatgcagg
    16981 gacattcttg ttgcaatctt taataaacgg aatgaattcg ttaggtaatt ctctttctac
    17041 tacaatgaac aatgtcgcta atacaatggt tggtggtata ggcaaaggag ttaatggcgt
    17101 catatcggga gttaactatg tgcttaagga agtcgaatca agtaagaata tcggaaactg
    17161 gccaattcca caatacgcta agggaactga tggacatcct ggtggacttg ctatgattaa
    17221 tgatcaaaaa ggcctagttc acgaagaata tgttcagatg ccagatggac gtagttttat
    17281 tgccaaaggt agagatcttc ttgttaattt gcctaaaggt gctcaggtct taaatgcttc
    17341 tttaacaaag aaattaaagg aacgtttaaa tattccacac tacgaaaaag gtattggcaa
    17401 tttagatatt gttgatttgt tagatgatga aaaaagaatg ttagaattct taactagcaa
    17461 agttgatttt tcaggcatta atgaaccatg gcttgatatg actaaatcag gaacgtcatt
    17521 aatgtccaaa gctgcaaata caatgcttca atcaaaatta agtgaattct tcactcatgg
    17581 caattttgat ggtgcagtaa atgccaatgg tgtttatcaa tatttagttg atgtggcaca
    17641 gaaagtaatg ggtaagttcc ccggacttac agtaacttct ggatatcgag caggagatgc
    17701 ctactatcac ggaaaacggc aagctatcga cttggcttat ccaggtattt ctggagatcc
    17761 gagatataca gcagcagcaa attacgcttt tgaaaaattc ccttcaaaaa ttgcgtatgt
    17821 cattacgaat ggacgtgtac gtgaccgcat gggattatct ggaacaaggt caagcggaca
    17881 atggacgaat tggccagatg gcgatcactt cgatcatatt catttaaacg gttcgatggg
    17941 gtctggcgat atattcaatg gaggagttgg aggaagtggt gttgcacgct ggcgttctta
    18001 tgtgtctaaa gcattgaaaa tgaatggatt accagctaca gcagcgtata taaatgcctg
    18061 gatgtcccaa atccaaacgg aatctggtgg taatgagaaa gcaatcggcg gcaatgatgg
    18121 gctcgccgaa ggcaatgcaa caggattact tcaaacgaaa cccggaactt ttgcagctaa
    18181 cgcttttcct ggtcacggaa atattatgaa cggatttgat aacatgttag cagcaatcaa
    18241 ttatgctaag aaacgttacg gagttgcagg tatgttgcaa gttatcggga aaggacatgg
    18301 atatgccaat ggcggcttga ttaccaaaga cggactatat cgagcaggag aaggaaataa
    18361 accagaaatg gttattccat tgacaagaaa aacaagagct atcgaactta tgggtcaagc
    18421 attagccttc ctttctggaa gcaataaaaa agaaacgaaa agtgagatta attctgtaga
    18481 taatagacat atcctacaag cgatagaaaa acagtctcgt atgacaaatg atctgctatc
    18541 attattactc tcgtttttta aaagtaacaa tcgatcagat aaagaactag ctttagatat
    18601 tcagaaaatt ttaatcagga ggacatagta tttgaggaca gtaatattaa gaaataaatt
    18661 gaatgaagaa attgatttgt ccacagaaga ttattttgct actgaaatgt cgaatctagg
    18721 ttttgaagtt acaaaagagc atattggaca atgggggagt ttcagagaaa taggagagag
    18781 tattgaattg tctgaatttc aatcatcagt tatgatttct gttcacggat ttagagaaca
    18841 acaattatat ggttcattag ttgaatttct ggctaatgga ccttttgaac tagaattttc
    18901 ttttgattca gaaacaatga tcaggaaatg tagtttgaaa tcattatcta aaacagaaat
    18961 tgattcccaa agctcattgc taacagatac tttgaatcta tattttactt caagctggta
    19021 ttctattaag cgtgaaaaat taatacagaa ttcaacttct ctcaatacac gtggaaaagt
    19081 ttactcattt gttcgaccat atgtctacac caaaaatgta tgggaaaaga aaggtgtatt
    19141 taagttcagt aataattctg tatatttgac aaatagtaca gagcgaatgt ctccgttaaa
    19201 aataaggatt attggagagt gctcaaatcc atattgggaa gttatccaaa actctaaagt
    19261 agtagcttca gatggctatt ttattaatat gaaagacaca caaatattag aagtgtctag
    19321 tttgctagaa gaccaaacgg caattttaaa agatgtaggt ggagttgaat cttctgttta
    19381 tcaacaacaa gattttacta aatcaaactt cgtccaagcg cctctaggag aattcagcgt
    19441 tgtttttcat gttggacatg caaatgtaga gattgaactt tatgaggagc gtgatctatt
    19501 ttgattttag ccattaccct ctttcaccga gatatgaatt tatataacga acgtttaatt
    19561 tcgactgagt tcgaatttgg aacggatgaa attggagaag aagtaagtaa ttttattaca
    19621 gataaatatg tccatgtaaa atcaggagat tttctactag caaaatttat tcctagcggg
    19681 aagattgctt attttggagt gattagctcg caagaagaca aaaaaataaa ttgcaaagga
    19741 ttactcagtt tggccgatag cgagataccg actgtaaaaa tatcaggtgc taattatgaa
    19801 gagcatattc gacgactgat tgaatactac ttattgaacg atccaacaaa acaactaaaa
    19861 aatattttgg aggtaaaaac agaaagcgtt acttctcatt cttatcaagc tgttgacaca
    19921 aataagagac gattgaatgc atatataata aatgggttca aaaaatataa tgttaaatgg
    19981 tatgtcaaag agattaaagg taaaaaaata tatactggta ttcgagcagt tgatgattca
    20041 atttatatta aagataattc atctgagttt aatgactggg atgtttttgt gcaatctcct
    20101 ggaccaggga acgagaacaa attattgatc gtagataaag caatgaaaga tatagaaaat
    20161 cccttggtat tgtctacgtg gtatttagat gacgaaaata atcttacgca agattcaacg
    20221 aataagaaca ttgcattgcc taccgttaat ttagtaaata tatatgatca aactcaagaa
    20281 gatagacctt cttatgaaga ggtagctaaa tcggagctta aaggcaacgc ctattcacat
    20341 gaaattaaag ttaatgttgt gctaaatgct agaaatatca atgtagaaaa tatcgaaaca
    20401 ggaatgttag cgactattgt ttacaaagag cagacttaca aatctgttct aactgcctgg
    20461 cgaatatcca gcagtaaaaa gaatatagaa ttgacatttg gtaatgtccg aagtcgtttt
    20521 atggattatt ttgaagataa tgggggataa aaaatggtta gcaatgtgga tggatatcaa
    20581 tttgaaaacg tgaaagtaag cgcagaaaat gatgctagac tttatcacgt tttatataat
    20641 cggaaaaatc aggttattga tggttacgat cagtctatga atttatcttc aagcggatta
    20701 acagtaaaag ttgctgcagg agcagcgatt attcaaggtc gtatggtcgt tgttcgacaa
    20761 gaagaaagta taacagttcc agcaaactca agtggttata tagcattaac agtggatttg
    20821 acacaagaag ttatacctgg atctattctt ccagaatcgg aagaatatga atggactaat
    20881 aatcaagtca agctagaatt tataacaaaa gttataaaag gtaatttaaa caatggtgat
    20941 aaggtctata atttaccact atgctcagtt aattctactg gatcaactgt ttcaatctca
    21001 aagatatcgg atagttacga gctgactctc tctaaaggag aaattttgtg gagggggact
    21061 gcgttaatgc atgatactca aactgtccaa ccttcaaaaa aaatttggca gacagtttca
    21121 ggttttttgt tagtatggct cccatacgaa aacgggcaag caattgagga tagatatgta
    21181 actacgcctt tttataagga gcgtataact tttactaatg ttttaggaga aattgtttca
    21241 ggatttgatg actatcacaa aaagtggttt agtaaacgaa taaactataa ttcgaatact
    21301 aatatattta caggtgcagc aagcaatgca agtggagata acgcaaatat ggtgcttagg
    21361 tatatagttt ctttttagtt aggaggtatg gaagagtggc aaatttagaa attaaattat
    21421 ctgcaaataa gagacagcct tatctacgtc accgtgttgt tggtagagtt ggtgatgggg
    21481 ggctcacaac aatcaatgta caacttcttg aggaagatga aattacacct tttgtaatta
    21541 atctaaacgg tactttgaaa tttgtgggcg aagtttcaaa cggtaactat accgagggag
    21601 aaccagaaat aatcgattcg actaatgggt taattagtta cacgttcact aagtcaaatt
    21661 tcagcacgag tcatcaattc aaacaagcat attttgaata tgtagatcct aacggcaaaa
    21721 aagtaacttt tcagaacttc atcatagacg tgttagaacg agtagatatt aattcggagc
    21781 aagcgaaata ctatatttct tcattggaaa aattacagag tgaaatgcaa accactttca
    21841 atcagttcat tagtgataaa caggtccaat acgatcaaat ctactcgaaa tataacgaat
    21901 tagtaagatt gataaatgaa tcagataagc aagtaaatga tcgtattgat aaggcaaata
    21961 atcgtattga tcaaaccaat cagcaaatcg gcgatctcgg caagctgaaa aagatgtaca
    22021 gtaacagcat tga
//
LOCUS       DACDSR010000041        21334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDSR010000041 DACDSR010000000
VERSION     DACDSR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.041"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..715
                     /locus_tag="IWK15_001562"
     CDS             14..715
                     /locus_tag="IWK15_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9547154.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1324..2340)
                     /gene="tsaD"
                     /locus_tag="IWK15_001563"
     CDS             complement(1324..2340)
                     /gene="tsaD"
                     /locus_tag="IWK15_001563"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9547155.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2337..2801)
                     /gene="rimI"
                     /locus_tag="IWK15_001564"
     CDS             complement(2337..2801)
                     /gene="rimI"
                     /locus_tag="IWK15_001564"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9547156.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2808..3350)
                     /gene="rimI"
                     /locus_tag="IWK15_001565"
     CDS             complement(2808..3350)
                     /gene="rimI"
                     /locus_tag="IWK15_001565"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9547157.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3334..4158)
                     /gene="tsaB"
                     /locus_tag="IWK15_001566"
     CDS             complement(3334..4158)
                     /gene="tsaB"
                     /locus_tag="IWK15_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9547158.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4247..5227)
                     /locus_tag="IWK15_001567"
     CDS             complement(4247..5227)
                     /locus_tag="IWK15_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9547159.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5251..6735)
                     /gene="galT"
                     /locus_tag="IWK15_001568"
     CDS             complement(5251..6735)
                     /gene="galT"
                     /locus_tag="IWK15_001568"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9547160.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6747..7736)
                     /gene="galE"
                     /locus_tag="IWK15_001569"
     CDS             complement(6747..7736)
                     /gene="galE"
                     /locus_tag="IWK15_001569"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9547161.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7984..8151
                     /locus_tag="IWK15_001570"
     CDS             7984..8151
                     /locus_tag="IWK15_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9547162.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8213..10024)
                     /locus_tag="IWK15_001571"
     CDS             complement(8213..10024)
                     /locus_tag="IWK15_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9547163.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10021..10386)
                     /locus_tag="IWK15_001572"
     CDS             complement(10021..10386)
                     /locus_tag="IWK15_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9547164.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10549..10944)
                     /locus_tag="IWK15_001573"
     CDS             complement(10549..10944)
                     /locus_tag="IWK15_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9547165.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10962..11924)
                     /locus_tag="IWK15_001574"
     CDS             complement(10962..11924)
                     /locus_tag="IWK15_001574"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9547166.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11924..12136)
                     /locus_tag="IWK15_001575"
     CDS             complement(11924..12136)
                     /locus_tag="IWK15_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9547167.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12157..12855)
                     /locus_tag="IWK15_001576"
     CDS             complement(12157..12855)
                     /locus_tag="IWK15_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9547168.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12875..13417)
                     /locus_tag="IWK15_001577"
     CDS             complement(12875..13417)
                     /locus_tag="IWK15_001577"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9547169.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13549..14553)
                     /locus_tag="IWK15_001578"
     CDS             complement(13549..14553)
                     /locus_tag="IWK15_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9547170.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14550..15539)
                     /locus_tag="IWK15_001579"
     CDS             complement(14550..15539)
                     /locus_tag="IWK15_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9547171.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15536..16342)
                     /locus_tag="IWK15_001580"
     CDS             complement(15536..16342)
                     /locus_tag="IWK15_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9547172.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16508..17464
                     /locus_tag="IWK15_001581"
     CDS             16508..17464
                     /locus_tag="IWK15_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9547173.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17541..18059)
                     /locus_tag="IWK15_001582"
     CDS             complement(17541..18059)
                     /locus_tag="IWK15_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9547174.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18147..18296)
                     /gene="rpmG"
                     /locus_tag="IWK15_001583"
     CDS             complement(18147..18296)
                     /gene="rpmG"
                     /locus_tag="IWK15_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9547175.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18524..18970
                     /locus_tag="IWK15_001584"
     CDS             18524..18970
                     /locus_tag="IWK15_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9547176.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19165..21060)
                     /gene="asnB"
                     /locus_tag="IWK15_001585"
     CDS             complement(19165..21060)
                     /gene="asnB"
                     /locus_tag="IWK15_001585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9547177.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGATFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6403 a         4543 c         3687 g         6701 t
ORIGIN      
        1 agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca
       61 acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct
      121 tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta
      181 tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta
      241 ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac
      301 ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta
      361 ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc
      421 taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga
      481 ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg
      541 tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac
      601 acgtgaaact gctggacaag taatcaaaca actacgtgat tccgggctgt tagattatga
      661 caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag aataacgcga
      721 acaaaaataa gcagtgaccc tgataaatat cagggtcact gcttattttc ctccctaaaa
      781 cttctgaaag atccggcagt tttagatcac tggacaagac tcaaagattt tctatgtgtg
      841 tttcagttat gtaatattca ctgtttgcaa aaaatactgt tagattaact agatgaagcg
      901 tcttgtcctc atccgatcta gattattcga tattcctcga caataaacga ttactcagtg
      961 agggcaatca gaggaacgga taatgataaa taagctgtaa aaaatagcaa aataacaata
     1021 atgattaaaa gtatgtaaaa aatagggctt tgacaaaaac gtgtcacagc cctatttcga
     1081 ttaaacggtg tgtacctgtc aactcttcgg tcacaagtca taatgttcat aaacatgagg
     1141 agctgttttt tgaaattgtt ccttgtgaat cagagttaaa cgacaggctt acgaactctt
     1201 gattaaacgg cgttcaaaag cttgcatacg cggtaataag aacagccagg tcaaaaacat
     1261 gagaagccgt ttttcctggg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     1321 tctttattca gataaaacca agcttggatc agcattcaac tgatagctag caaaatttct
     1381 tttttgcatt tccacatgtg ctgctgctcc aatcattgca gcattatctc cacaaagtcg
     1441 aagaggggga atcactagtt ctacctcagg gagtttagct gccaaagcat cctgcaatcc
     1501 ttcacgcaac ccttgattgg ccgctacacc tccagctata atcagttgct tgactggata
     1561 attttgacaa gctcgaagtg ttttagtaat caatacatcg atcacacttg cttgaaagct
     1621 agctgctaaa tcatttttgt ctagatcttc tccacgctgt tgtgcattat gcaccaaatt
     1681 gataaatgca ctttttaatc cgctaaaact gaaatcataa ttatcttcat ggatcatcgc
     1741 tcgcggaaaa tggtaattat cttttccctg atgagctagc tgatcgattt cttttccgct
     1801 tgggtaagaa agacctaata ctcttccaac tttgtcataa gcttctcctg ctgcgtcatc
     1861 tcttgtctcc ccaatgattt catatgatcc atcttcttgc atatagacta attctgtatg
     1921 tcctccgcta acaagaagtg ccatcaatgg aaactggaat ggtttaacca gacgggctgc
     1981 atagatatgc cctgccatat ggtttacagg aataagcggc aattggtgtg cccaagcaaa
     2041 agccttagct gcagaaatac caatcagcaa cgagccgaca agtcctggtc cataagtgac
     2101 agcgactgcc gataaatctt ctgccgacac accagcttct accagtgcat cttccaagca
     2161 taaggtgatt tgttctacgt gatggcggct agctacttca ggtactacgc caccaaaacg
     2221 tttatgactg tttatttgag aagcaacgat gttagataag atctctgttc catttctaac
     2281 gaccgctaca ctcgtttcat cacaacttga ttcaatcgcc agaatcaatt cttcattcat
     2341 ttcatcgtct ccgtttttaa ctttctgctc ataatgatcg catcttccgt cggattgcga
     2401 taatatgatt tccgtttacc taatatacga aacttttctg attcataaag ttttcttgct
     2461 gcttcattag atagacgtac ttctaaaaaa acatgcagcg aatcttttat ttgttccgta
     2521 atcagtaatt gtaacaaatg ccgtgcatgc ccttttcctt gttctgaaac tgcaacagca
     2581 atatttgtga tctcgacttc atctattact ttcgagtatc caataaagcc acagatctgg
     2641 ttatcttcag ctaaaagcag ataatcagag tgaggctgtt ggatgtctgc caaaaactgc
     2701 attttcgtcc aaggtgaacc atgtggataa gcattttcgc taatcatcca tacgcattct
     2761 gccaagtacg cttcatcaaa atcttttttt ctgcaaatca actaatctca ctcattttct
     2821 aaaagtttta tcatatccaa cgcatcttct tttgtttcag tgtaatatct ttttttgatc
     2881 gttgttgctt gaaaccccaa tttccgataa agtctttgtg catcttgatt actcatacgc
     2941 acttctaatg acatcgtctc acagcgattc attatagcaa agttctcgat ttcgtcaata
     3001 agcagcgaac cgattcgttt acgctgatat gccggtcgaa cggcaatatt cgtgatatgg
     3061 caatctaaac cgaacgctct tgaaccgata aaaccaatca ttcttccatt atcaaagata
     3121 cctagatata gatgaggaag cggtgacttc aattctgata taaaggcact ttttgtccat
     3181 ggcaattcgc cattatagac ttctcgttcg acatccagca gctctttgat atcttctgta
     3241 gtcaattctc ttagtacgta acgacgatcg atttgttcga ctgttttttc tgtataaatc
     3301 attttatctt gaaaaaagct tctggcatac gctctaaatt ttttcaacat aactttcgtc
     3361 tccaggtgtg tgcgtctcca accatttttc ttctgcttct actcgtttta aatagcgagg
     3421 aagaaaagct tgtttctctg caactggttc aactaaagca cctaattgag ccacagttac
     3481 accacttgga atatcccatg ctggaactgt attaatcatc gcactaggca atatttcctt
     3541 tatttcagag aagaatttgt gacaatcact cccgacgaaa taaatagctt cattcagctt
     3601 ttgtagttgt gtgaacaaat cagaagcaga aatatgtaca tcttcaagta cagtttctaa
     3661 tctattttct ttccagcggt atgccccagc gtatacattt tttcttcgag catcaaacat
     3721 gggcacaata atattatcca caccgatgca attagccgcg atggttttca gactagatac
     3781 tcctactagc tctttatcta atgtataagc aatcgttttt gctgtcgtta cacctatacg
     3841 aagccctgta taagaaccgg gaccatcaga aaccgcgatt cggtcaatgt cttttggact
     3901 tatttgaaga tctgcaaaca attggtcgat tgcaggcatc agtgttacac tgtggttgcg
     3961 tttgatattt gtttgtattt gtccaagaat ttgttcgtct tctaccacac cgatcgccaa
     4021 agtttggttt gcagtatcta ttcctaatgt aatcatatat ttgatccttt ctgctatgac
     4081 attctcgcct tccattgtag catacttttt tattctgagg atgaaaatac caaaggcaaa
     4141 actagtacaa taatacatga ttatctgctg gcgggcaaat tttgcccctt cctgaaacac
     4201 aaaaaagctg cggtatcact ccattttccc acagcttttc catcttttat ttcgttgatt
     4261 ctcgaataat caactctgtt tccacaataa tctttcttga aaccggtgct ttggaattac
     4321 ttaattgaag aatcgtctcc actgctagct ctcccatcca ttcggtataa acttttacag
     4381 tagaaagcga gggcgttaca tatttcgcta cactgacatc attgaagccc atcacagaaa
     4441 tatcttctgg taccactaaa cctttttctt gaatcgcttt cagtgcgcca atcgctaatg
     4501 catcattagc tgcaaaaagc gcatcaggaa catgcggatt cgtttttaaa tattgcttca
     4561 ctgcttgata tcctgactcg acagaaaaat ctgcttcgat aattggacct tcgtcgtagc
     4621 ctttttctgc caaaacttgt tgaaatgcca taattctggg atcttttagc cgctgatggt
     4681 ttttcttcgt atattctgtt ccagcaagca tagcgatatg ttgcgcttta tgggagacaa
     4741 agtgttcaat gactttttga acacttgact taaagtcgac aactagagag tcgattcctt
     4801 gagaagtgcc gtcagaatct accaaaagca aggcattttg ggatcctttc atttctaata
     4861 actgagcttc gtcgaatttt cctagtgcga tcgttccatc tgccggaaga tcactaatgg
     4921 aatcccacgt ttctttcaat aaagtaacgt ttgcttcttc tgcctttttt tcgattccta
     4981 ggcgaataga taaataataa agatcttcaa gctcttccgt atcgtcgtac cactggatca
     5041 ggcgaagcgt ttgcttagta tttgctgcat ttttacgatg tttcgtataa tttaagcgtt
     5101 ctgctgcttc aaaaattttt tgctttgtgg ctaagccaac ggaaagatca ggatcatagt
     5161 tgagaactct tgaaacagta gcaggagaaa ctcctgccaa ctgcgcaatg tctttaattg
     5221 ttgccatttg tctctcctcc ttttttacgt ttataaaatc gcagtaaatc gttcaaacgc
     5281 tgcctgtcct ttttcatctc gcttgaatac tccagcgtct tccagtacac gtgcaaaaac
     5341 ctgacctaca gatttttgta cgacatcggt gaccgtatct ttcgtcaaat caggatgttc
     5401 tttttttaat tgttctgccc acggacgatg atattcagcc atctggtttt cttctcctaa
     5461 taagaaatgc tctacttcta ctagttctgg tttcaaacga ggtggtaaaa cagcaagccc
     5521 cattacttcg atcaacccaa tgttctcctt tttgatatgt tggacatctt tatgtggatg
     5581 aaaaatccca tcaggatgtt cttctgaaac attgttgtct cgaaggacaa ggtccaactc
     5641 aaagtaattg tcccttcttc gtgcaattgg cgtaatcgta tggtgtggtg taccatctgc
     5701 tgaaaaagct ctgatagata ctgtttcatc cgaatatgct ttccattttt ccaatactga
     5761 gttagctgct tcaataagca agtttttctc tgttgattgc aaacggatca cagacatcgg
     5821 ccatttcacg actcctgcga tgacttgcgg ataatcttct agtttgataa gtttttctat
     5881 cggtgctttt gccataggaa attcatgacg tcctgcctga taatgatcat gagaaagaat
     5941 cgatcctcca acaattggta agtcagcatt cgaaccaaca aagtaatgag gcaagacttc
     6001 agtaatccga agcagtctag caaatgtttc ctttgtgatt ttcatcgggc ggtgttgttc
     6061 tgataaaatg atcgaatgtt cattgtaata agcataaggg gaatattgga atccccagct
     6121 ttcaccgtct agattcattc gaataatccg gtgattggtg cgggctggat agttcaagcg
     6181 acctctatat ccttcgtttt ccatgcaaag catgcaggta ggatagtcta cttttaccgc
     6241 ctcgcgttct gctgcgattt gttttggatc tttttctggc ttagataaat tgattgtgat
     6301 ttccagttca ccatactccg actctgccgg aaatttgatg ttttttgcga tggctcttgt
     6361 tttgatgtag tcatttttct tacttaaatt aaagaaatac tcagttgctt cctctgggct
     6421 tttcgaatag tgctgtgcaa agaatgcatt gacgactgaa ggtggcggtg tgagaaaatc
     6481 cattaattgc gcttcaaaaa tctcttgttc tgctggcaat tctgaaagaa caccattttc
     6541 tcttgcctta gcgacaagct gatccaataa gtctacagat tcttccttaa ctggtcttac
     6601 ttcaatgctt tctagagcat cttctcctac catccctaaa agacgattct gtaaatataa
     6661 gcggtccatt tccatccatc cgccagcttc gatcgcaagt gtggcaaaat cacaaatcgt
     6721 ttgactgatt gacatatctc attcccctat tcttcataac catttggatg gctgacatgc
     6781 caatcccatg ctgttttgat aatcgctttg atatctgtgt attcaggttt ccagcccaga
     6841 atctctctcg ctttttcact tgaagcgact aatcggctag gatctccagc tcttcttggt
     6901 gctatttctg ctggaatttc ttttcctgtc acttctctag ctgcttctag catttccttg
     6961 actgagtatc ctttgctgct gcctaaattg aagaagttgc tttcatttcc ttctttcaag
     7021 tattccaaag ctaaaatatg tgcagcaatc aaatcttcta cttgaacata atctctgata
     7081 catgttccat ctggtgtatc ataatcgtct ccataaacag ccaaggcttt tctttgacca
     7141 agagctactt gtaaaatgat tgggacaaga tgcgtttctg gtgtatgatc ttctccgata
     7201 gatgcatctg cttttgctcc ggctacattg aagtagcgca aagcgacata gcgcatgcca
     7261 tatgcttggt cacaccactt catcattttt tccatcatca atttactttc accatacgga
     7321 tttttaggat tagctggtgt atcttctgtg attggagact ctttcggctc tccataagtt
     7381 gctgcagtag aagagaatac gattttattc acgttgaact cgtgcatgac ttcaagtaag
     7441 atttgcatac cgtatacgtt gttgttgaaa tacatcaatg gtttttcgac agattctcct
     7501 actaaagagc ttgcagcaaa atggattact ccttcaatcg gttccttttc aaatacggaa
     7561 cgtagaaatt ctttatcccg gatgtctcct tcataaaaat gagcatctgg atgtactgct
     7621 tgtttgtgac ctgtgagcaa attatctacc acagctactt gatacccctt ttgaactagc
     7681 tggtctacag cgtgcgaacc gatataaccg gctccgccta gtactaaaat tgtcataaaa
     7741 aacactcctt tgattagtaa aattgtgttt gttgattcta tataatttaa aaaacgaagt
     7801 ttacttttat ggtgaaaaca aacaaagtaa aatttcgtaa attcatatgg taaatttata
     7861 ataaaacatt tagtaaacat tttcaagggt tttttgcaaa ggtttacaaa caaacgtaaa
     7921 actagccttt ttttctcttt tgttccataa tagatataac aaataataaa tgaggtggat
     7981 tcaatgaaaa aatttgtatc tggaatatta gtaggaagtc tggcaacagc cgctgccgtt
     8041 gctggtttgg ttgcttctgt gaaaaaaaca gtgatcgatc caattgacga aaaagaagca
     8101 atgatcgaag aaaaccgtaa aaaagcaatg cgtaaacgcg tttctcgtta ataattattt
     8161 aaaaatacgc atgaagactt tctaaaaaac agataagtca tcatgcgtat ttttatttgt
     8221 cagtcaatat aagttgtgct attttatctg cctgtctcag attccatgga tctgtataag
     8281 gtctagctac tgctgcagtt agccaatcca caccttcaac aggtattcca tagcaataaa
     8341 agtcagactg gatttcccct gaagcatcaa tcacgcgatt cgtacctcgc tcaacatcta
     8401 tagcttctga atgataactt cctgtattat ctgaaaatga aaaagggcga acaagtttgt
     8461 tttccttcaa attttttatg acaggattac ttgaacgaga aaagcttgtt tgtggcaatc
     8521 gagcttcaat caaatagcga ctgctgattt ctttatcttg tagagaaata acatagcggt
     8581 tgtgcttttg ctgaatatca aatggcggtt cgatcaaatg aaaaataccc gcatctatca
     8641 gtgctgctaa ttctttcgtc cgaatgattg gcggaccgat cgttaaaaag ctatttagat
     8701 gagtaaacca tccccatagt atttctttgt attccttagc agaaaaaacg tgccattcaa
     8761 tagcctgccg gattggatca cgccagtctt ttaatgcatc aaatgttgaa gcaaatgctc
     8821 ctgtgcaatt gcctttttcc gcttctttta tctgcccttc aataaatatt cggctagctt
     8881 ctgaaaaggg ctcattctga taggcaagag gagtttctaa atacgaccaa tcccatctat
     8941 aagggattag tgctggatgc tttccgagac actcctctga agagagggag agaaaatcat
     9001 gctcaaaatt tttctttgga atcggcaagt gatattcttc gatgattttc ttgtaataaa
     9061 acaactcaac gtcttttttc agatagtcaa aaaatgtttc gccagtcaaa aatcccgact
     9121 gatgccattg ctccaggttt tcttttgtta gtaaccgtgg ccatgccatt gctccgcctt
     9181 gcttttgatt tcttcctcga gggtaataag ggagccccct tcttgatcca ctgtatacaa
     9241 tcggttcctt gcctgatgga tgatagacca gttttccctt ttcgttctcg aagcgccctc
     9301 ctcttgatac tgtaaacaat ccgacataat caaaaaaact taagcctaga cctcttaaaa
     9361 agacattttc tttaggcggt atcttcgaaa gatctacatc tgcaggatta gaaggaagct
     9421 gatacagtaa aacatttttc tttgcgtagt ctacccacac tctttcttct tctgtctcat
     9481 cattttccca gtgaccgctt gctaaaacaa gctgatcgat agaatagctt tgctgatctg
     9541 tctgtaaaag ataaccatct tttcttttgc ttactttttt tactaaagaa taaacaaact
     9601 gaatagaagc tggaaattct tcttgcaact ttgagaaaaa ccagcgttga tagagtccgt
     9661 ataaacatcg agtagattgc tgatcttttt ccagacgatt gatttcttct ataattagtt
     9721 gcttattttt ggttaccgtc tgttcgatat aatctgtagc aggtccttta ctccattgaa
     9781 acaagttggg tccctgtgtt accaatcctc cactggacaa tgtttcatct gtaaaaagcg
     9841 tcacttgttg agaaacagaa ttcattaata actccggtgg ctgattcaac cgccaaactc
     9901 gacctccagg accattggga tcgaaaagaa aaagttcaag agaatgtctg tcctgattat
     9961 tatggagaat cctttctgct acacttaatc ctctaggtcc ggcaccaatg atccctattt
    10021 tcatttgcat gcctccattt cgataaattt tttcaataaa acagcatggt tctgatgttc
    10081 attttttgca ccataaacaa aagtaatgac tggatgttct ttcataagtt gactgagttt
    10141 aaaaatagct tgcttctttt cttcttcgaa aatcaattct tgctggtatt tctttgcaaa
    10201 tgaagcaaat cgttctggta catggtcaaa agttttgcgt gcttcattac taggtgcaat
    10261 ttctttcaac cacagatcta actgcgcttt ttcttttgaa attcctcgtg gccagaatct
    10321 atccacgagt attctatagc catcctcttt ttcagcatca aggtaaacac gttttaattt
    10381 gatcatagtt gtttccttct ttctaccttt tcgcttagct tatatatgta gataatagca
    10441 caaaataaaa aaaatgaacg gcacaactat ccattgcgcc gttcatgtga tcttggtcaa
    10501 aatctaatga ttgattcggt ttttaaagct gtcatacaat catctgtttt atactttttt
    10561 acttttgatt tttccagacc atccgtcata tccaccctta agtaaataaa tctcttgata
    10621 acctgcttta cgtaatttat ttgctgtacg gatgctcaaa cttttctttt gatcgtaaat
    10681 atagacaggc tggtcttttc gaatagatcc taatgaatct ttcaaaacag tgtaagggat
    10741 gtttcttgca cctaaaatat gcccagcatc aaacacatct ttttcgcgaa catcgattac
    10801 ttgtgctttt ctcattcctt ctcgaaattc ttcttctgtc aaagtcgttg ctgaacgttt
    10861 ggccattact cgtagataga cttcccaaag gatgattgca agcacaatca atagcaaaat
    10921 gatattaatt acccataaaa ccatgtaatg cctccgtttt tttaagcaaa tttcaaggct
    10981 aatgaggcag caccaatgac accagcttca ttaccaagct ctgctaattt gatttttgtt
    11041 gattctctta cttgcgggaa agtaaattct ttaaaatatt tctctacacg gctacgtaaa
    11101 aattctcctg ctgccgatac tcctccacct aacacgatac ttgacggatt taatgtattc
    11161 cccaagtttc cgcaagctaa acctaagtag aagcatacgc gatcaacaac catcaatgca
    11221 aacggatcgt cttcttgagc catttcaaag atatctttac ttgttacttc ttctccgttg
    11281 tctagcattg ctttcagttt tgaatcccct gcgtattctt ctgccaagaa acgacctagg
    11341 cgtaccacac ctgtagcgct agatacagtt tctaaacatc cgcgtttacc gcaagtacat
    11401 tcgaaccctt caggatccac agtgatatgt ccgatttctc ctccagctcc gctcacgccg
    11461 tgaatcaaat ttccgccagc aacgatacct ccgccaacac ctgttcctag agtaacgaat
    11521 acaacgtctg gatcgttttc tccagcacct ttccatcgtt cacctaaagc agcaacattc
    11581 gcgtcattat cgatagcgaa tttgattcct gttcctgcct cgatcagttt tttcacaaat
    11641 tgaacttctg tccagtttaa gttatacgca ccaataaccg ttccagcttc gctgtccacg
    11701 cttcctgggg tacccatgcc gattccgata aagtcttcgg ctttcatatt atataaattc
    11761 aaacgatgat tgattgattc aatgatttca ggaacgatat gcttaccatc atccaaaata
    11821 ttggtatcga tgctccattt ttgctgaatc tctccttctg gtgttaaaat tgcaaatttt
    11881 gctgttgttc cacctaaatc aatcccaata atcttcttgt ccattacaaa tttcctttct
    11941 ttcggctttc ttcaattcgg tgctctcttt ttaatatgcg ccacgctgtc atgtatgtat
    12001 catgatcaat caaacggcta tcatacagat tacgcaattc gatagtcatt aattcaatgt
    12061 catagatgcg tgcacccaca tagataaaga tgccaaattt tttgaacagc tgctgtacat
    12121 catataagct ctccatcttt aagcacttcc ttcatattat acaagtaaac catatttttt
    12181 taaacccagt aaaatacata ttacaaaaag aaatacaaaa actagtgtag aaagaatcct
    12241 ttggtgtatc gaatagcttg tctgttgttt cggtagaccc aaaacattcc ctaacactaa
    12301 tccgccaatt agcccaccga catgtcccca aacatctaca gaggtatcca gaagaccaaa
    12361 aacaaatgtc aacacaataa ataaaagaaa atgtctgact agctgctgga tcgctgggtt
    12421 atgtttaaaa tgaaacccta agataaacaa cgcaccgaac aaaccgaata tcgaagtact
    12481 tgcaccacca gacagaacat tcgcttcgtt aaaagcaaag cttgctgtat ttcccattat
    12541 cccgctgaac atataaatca agaaaaaacg ccaatggcca tagattgcct cgatttgctg
    12601 ccccataaaa tacagtatca gtgagttcat tacgaaatgc atcagtccaa aatggatgaa
    12661 gattggtgtc actaaacgcc accattcatt gaagtgaaca agataaggac catacattcc
    12721 ggcataataa gggatctcaa gtcgcggaaa taattccatc atgaggaaga ccgctgtctg
    12781 tatccctaaa aaagcatagg tccataacgg tcgtcttaac catgcacgca attttatttg
    12841 ctgttgataa ttcatgaaaa cctcccattt cattttagtc aataaacaat tgttggatct
    12901 tctgatcaaa gctctccggt tgccattgtt cattcaattg ttcacgaaaa acaaggctac
    12961 atgttttttt atgaaaatta tttaaatacc ggtcgtaata accaccgcca aaaccgattc
    13021 gataaccggc tctattgtag ataatacctg gtacaatgag gagatcgatc atctccggca
    13081 aatagagctt ctctgattgt ggctcaagaa caccaaaact cgaacgtaca agaacggact
    13141 gttcatccat ttcataaaac gccatctgac gtttcggcaa agttttaggt acaactattt
    13201 tcttttgttc tttttctgct cgttggataa ccggctgtgt atccagttca aattcattcg
    13261 ataaagtcgt ccctatcact tttgctgact tccagtactc gctggcgaat agctgtttat
    13321 agattcgctg ttctttttcc tttttcttgc cacttttggc cagatttttc agcttttcga
    13381 ttcctttccg gcgtaatctt actttcaatt ggtccaaaaa ctcacccttc cttctctcat
    13441 aaaatggtat aggaaaaaaa gaaaaatagc aatcgaactt tcaaataaat gcataaatag
    13501 aataaaaaga agacctagaa atgattggcc ttcctttttt aatgaatttc aactgttcag
    13561 cgcgtgtttt tgtctgataa aaagtgcata cagaaaatat ggcgcaccaa aaagggcaat
    13621 caaaattcct gtaggtactt ccccacttgg tagaatcact ctagcaacca tatctgcact
    13681 aagcagaaaa atggaaccga acaaaccgct cagaacaaag gtccagttat tttctttacg
    13741 tacgactagc ttcgcagcat gaggagctag tagtcctaca aaagataaac tacctgtcac
    13801 cgctacacta ccgcttgcca aagcaacagc taacaacaaa aggatttttt gacttttagc
    13861 cacgttaagg cctaatcctt gcgcgcgttc ttgaccaaat gatagcaata caatcctctg
    13921 tgcataaagt cctcctagcg gaattaagat aaccgcccat ggaagcaatg cccaaacata
    13981 aggccagctc gcaccccaga ttgatcccgc taaccatgca ttcacaaatc cataattttc
    14041 cttagataat tggatcgtgc atagcaatgt taatgcagaa aggccggcat taacggcaat
    14101 accggacaat aagattcgat tcatacccaa aaagccattt cgatcatagg ccgcaagata
    14161 gacacagcaa gcagctaaaa caccacctag aaaggctaca actagcaata gaaacggcag
    14221 cgaatagtgc gaaaagaaac ctaaataaag catcactaaa aatcctgctc cggcattaat
    14281 gcccaatata ctagaatctg ccaattcatt tcttgttact ccttgcaaga tataaccaga
    14341 aacgccaaga caagctcctg ctaataaact gatcagtaat cgaggaactc gaaattctct
    14401 aacaatcagc gccaaagaag atgattcttt ccctatgaac gtggcaaaaa gctgatccag
    14461 gtaaataaaa ggcgtaccaa tcattaaact acctatagac aggatgacaa gtacaaaaag
    14521 caagaaatag aacgtccaat atattttttt caccgttgtt ccctccttac ttgtatcagt
    14581 aaaaacggaa cgccaatcaa tgcaatgact aatccaaaag gtgtttcaaa agggggattg
    14641 atcatcctcg ctgtaaaatc cgccaacata accaatccac cgccaactaa aaaagagaca
    14701 ggtagaacat aacgatagtc cgttccaacc attccgcgaa caactggcgg aaccaacagc
    14761 ccaataaatg aaacaggtcc gactaacgaa acggcaatgg ctgctaacac tgcaactaga
    14821 cagaaaacaa tcaaccgcgt tcgtttagga tgacttccaa gagctaatgc atgtgtttca
    14881 ctcaaatgaa tcaaagatat tgcttttcct aatgaaaaag aaaccaaaac agcaagtata
    14941 tataaaggaa acaatactgc taattgtttc catgtgatat tggcagctcc gcctacaaac
    15001 caaaaagcaa gatcctgctg cagattaaag aattgggtaa gtgcttggct gatagcagta
    15061 aaaaaagaac taatagctac gccagctaaa atcagctgta aaggattcat gcccattttc
    15121 gaaaactgca aaaaaccaaa tacgagcaaa gttacagcaa aagaacctgc caatgacatt
    15181 agaaacacag aaaaatcaga agctttaggc caaaaagcaa aactaagggc cattgccaac
    15241 gaagcacccg cgttgattcc tagtaagcct gaatctgcta aaggatttct tgtcacccct
    15301 tgcatcaatg caccgctcaa tgcaaaacaa ctccctacaa gaaatgctcc cgccacacga
    15361 ggcaaacgaa tatttcgtat gatttggtga ctttgttttg atctatcaaa atcaaacaac
    15421 gcctgccaaa aatctgcttt ttccacagtc gctgcgccat ttgaaagagc aacaaaaaaa
    15481 ataatcaaaa gaacaaaaca gaccaaaagc aggagtctat ttttcttcat agtcctcata
    15541 attgatcctc ctgctctttt ggcaacaaat cataggttag aaaaactggt ttatttgttc
    15601 ttggaagcgt gacaatcgtt gcatcaatct caaatatttt tttcatacac tcttttgtga
    15661 tcacttcttc aggtgtacct tctgtgatca acttaccgtt tttcattgct aataaataat
    15721 ctgaaaaacg tgaagcatga ttaatgtcat gaatcgacat caagatcgtt gtttgtttca
    15781 tttgattgat ttcagtcaat aactgtagga tctctaattg gtgtgccgga tctaaaaatg
    15841 ttgtcggttc atccaagatc aagatatctg tttcctgagc aagcgccata gcgatccaga
    15901 cacgctgggt ttgtccacca gacagcgttg cgatatcccg catacgaaat ccagctagac
    15961 ctgtagcatc caacgcccac tcaattgctt catgatcttg cgtgtccatg ccactgaaca
    16021 gtttttgata agggtatcgt ccgtaagata ctacttcttc tactgtcata cctgtagggt
    16081 gttcactagt ttgtgctaat aatgcaattt tttttgcaag ttcttttgaa ctgtatgtag
    16141 agatatcaga gttattgaca atgatctttc ctgatttcac aggtagtata cgggtaaaac
    16201 ttttcaataa agtagatttt ccacttccat ttggcccaat caaactagtg attttccctt
    16261 caggaatcgt aacagacatg ttttcaatga tttttttgtt ttcgtagccg gctgcaatgt
    16321 cagctgctct taatctcgtc atttcaccca tcctttcgct gaaaatcatt ctcaattatg
    16381 acgctgtttc aacatttcgt caagcgctac tttcatatct atagccaaat gaagcggttg
    16441 acagcgttcg aaatatcagg aataataacc aatgagaaag attctcaatt atgttaggag
    16501 gaaagaaatg aaattaaaaa caaaagtatt cacattatct agcatattgc tgctggctgg
    16561 agtactgagc gcttgctccc cctcttcaga taaagaaaca gctacaactg ccaattcgac
    16621 gacttcatcc aatagtactc atacagtcac agatacactt ggacataaag tagagattcc
    16681 aaacgcgccc aaacggatca tcggcagtta cttagaagac tatctgattg cactagggga
    16741 aaaacctatc gcccagtgga ctgttggcag tggttccatt caagattatc tgcaagatga
    16801 tttaaaggat gtcccaacga tttcttatga ccttccttat gagaaagtgt taagctttga
    16861 gcctgatttg ctgttgattt cttcttccgc tactgtcgaa ggcgggaaat acgaacaata
    16921 tagcaaaatc gcgccaactt atgttgtaaa aaacggaaca gatgtcacat gggaagaaca
    16981 actaaaagat gtcggcaaag ctttggcaaa agaaaaagag gcagaaaaaa tcatttccga
    17041 ttatcaaaaa tccatcaaga aaactcgaga agaactagca gataaaatcg agaacaagac
    17101 agctgcagtg ttgtgggtga ccaacaactc agcctttatg gtcagcaaaa atcggtcaag
    17161 tggaagaatc gtatacgacg atctggaatt tggtgttcct gatctagtgg aagaggtctc
    17221 caaaaaagcc acttctgact ggtctgctgt ttcctctgaa aagctagctg aattagatgc
    17281 tgactacatc atccttgtga acagtgacaa agatgctgcc atgttcaatg aaccaagctg
    17341 gaagaatcta aaagcagtga cagacaatca tgtactgcaa ttcggacctg aatctagttg
    17401 gctatataac ggacctattg ccagtcagga aatggtcaat gatatcaaag aaaaactaaa
    17461 ttgattttaa ctaaaaaaag catcttcacg attagtcgtg agatgctttt tttcttatta
    17521 acaaactaga cgcatagatt ttattcttct tttgaagcaa ccctattttt tactctttcc
    17581 catttttgaa aacgatgggc atatagattt tcttgatccg ttatcccctc atactcctct
    17641 ataagggcga agtctgaaaa atcaatattc ccaatatacg tatcgccttc aaacgtgcta
    17701 tcgatcaatg ttcgccagat ccgaccagtg taaggaagaa gttcttgaaa aatccgacta
    17761 ccacccgaaa catagatatc ttctgagatc gtttgactat acgacaaaag ttcatcgatc
    17821 gaatgcatta cttccgcatt ttctttcgtg cgaaaatttg gttgcgttgt caacacgatg
    17881 atatggcgat aaggaagtga aagatccccc attccttcat aggtctttct tcccatcact
    17941 aaaattcgat ccattgtatg ttcgcgaaag aaacgcatat catttggcaa tcgccatggt
    18001 aacaaaccat ttttgccgat cagaccattt ttatcttgtg cccacatgga aataaacata
    18061 ttatccgctc cttatgattt tagatgtttt gagataaaaa aagtctcaat tctttgacca
    18121 gcaaggaatt gagaacttta aaaacattat tttgtttcac ggtgcaaagt aactttgcgt
    18181 tcgcgtgggc aatatttttg tttttccaaa cgatcaggat tgttacgttt gtttttgctt
    18241 gttagatagt tacgttcttt acaagaagta cactctaaag tgatgtttac gcgcatgttt
    18301 ttccctccca tttttacaag aatcctctaa gatgactctc agacttgatt atcttaccat
    18361 gattttttct agattgctag tgtttttcac gattttgtta agtaaaacta cttgacgata
    18421 aatacaacgg ttttacttct caaacaaaat ctttttcttt ttgtataaat acccttacac
    18481 ttgtttatat agattatgtt ttttaataaa gggggatact aatatgccat ggaacatgaa
    18541 ggactttcct gcatcaatga aaaacttaga taaattgaca agaaaaaaag caatcgacat
    18601 cgcaaatgca ttattagatg aagggtaccc tgataaccgt gcgatcccaa ttgccattga
    18661 tcaagcaaaa aaatggtatg aagatgctag tgaatcagaa aaacgaactt tcgaaaaaga
    18721 aaaaaatcca agcaagacgg atgaacacga tacgaatcct cgtgctggaa aactgcttga
    18781 ttcagatgtg atcgtagaat atgaggaaga acaatggatc gtgaaatcta aaggggcaaa
    18841 aaaggcaagc aaccattttg atacaaaaaa agaagcaatc gaaaaaggaa agcaagtagc
    18901 acaaaacaaa gaatcaactt tgttcattta taaaaaagat gggacaaaag aaaaagaaat
    18961 cgattattga acatatgtaa aggggttgtg acgaaaatcg tgtcacaacc cccttatgtt
    19021 cgaaacaatg gtgttcctct gccaactccg aaccataagt cataatatct ataaaacggg
    19081 aagagctgtt ttttgaaatt gtttcttatc actcagtgtt aaacggtagg agaaaacctc
    19141 ttttttattg atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg
    19201 aatcgtttca tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat
    19261 ttttctttgc tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc
    19321 ttgatcaaaa aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc
    19381 gtgttcttct ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga
    19441 ccattcttcc ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg
    19501 attcaataaa aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa
    19561 aggtactcgg acttccaaag aacttgccat agaaagttta tctgccttta acaagatatc
    19621 ttttggcatc cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc
    19681 ttctgttttc tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt
    19741 tggtgttaac acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata
    19801 ttcttttgca ggagcaagtg attcatataa atggatccgt ccatggaagt ttttcatttt
    19861 tccgatttta cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc
    19921 aatcacacgg atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag
    19981 ttcatccgcc ccttctccag attggactac ccgcacactt tgtgaagcaa gatttgctaa
    20041 aaagtacaaa ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa
    20101 agggaatgct ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaagtccaa
    20161 tagttcggtt agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat
    20221 tgagaaagaa tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga
    20281 taagaaagaa ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt
    20341 ttcgtcgatt ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc
    20401 atcccaatat tgctgaatgt ctatttttcc atctttatac gtataataat ggccttcagg
    20461 caaacgatag acatctttga agaatgttgc gccgtttaat ggagaatatt ggaaagtcat
    20521 ataaggtttc aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact
    20581 tttgatttct gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat
    20641 cccaaagtgg tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc
    20701 aaacattccg cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag
    20761 gacttctgta tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa
    20821 aggctggtaa ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta
    20881 aataggctga gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac
    20941 atgtttatcg atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat
    21001 tgtattaata attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat
    21061 ttaactttca catcctaata taaaattact gtttatttca ttcatatgca tcttatcata
    21121 gaataaatag cggacgaata ttttctctct tacaatttcg tcacgtgaaa acaaaagatg
    21181 aaagtttctg tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt
    21241 tgaggctacc ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa
    21301 ccagcaaaaa aagacacgac ctaaagatcg tgtc
//
LOCUS       DACDSR010000042        20954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDSR010000042 DACDSR010000000
VERSION     DACDSR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.042"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            190..564
                     /locus_tag="IWK15_001586"
     CDS             190..564
                     /locus_tag="IWK15_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9547178.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(678..1307)
                     /locus_tag="IWK15_001587"
     CDS             complement(678..1307)
                     /locus_tag="IWK15_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9547179.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1441..1513)
                     /locus_tag="IWK15_001588"
     tRNA            complement(1441..1513)
                     /locus_tag="IWK15_001588"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Thr,seq:ggt)
     gene            1727..1800
                     /locus_tag="IWK15_001589"
     tRNA            1727..1800
                     /locus_tag="IWK15_001589"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1761..1763,aa:Asn,seq:gtt)
     gene            1755..1904
                     /locus_tag="IWK15_001590"
     CDS             1755..1904
                     /locus_tag="IWK15_001590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547180.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2397..3725
                     /locus_tag="IWK15_001591"
     CDS             2397..3725
                     /locus_tag="IWK15_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9547181.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3927..4760
                     /locus_tag="IWK15_001592"
     CDS             3927..4760
                     /locus_tag="IWK15_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9547182.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4776..5513
                     /locus_tag="IWK15_001593"
     CDS             4776..5513
                     /locus_tag="IWK15_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9547183.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5668..6636)
                     /locus_tag="IWK15_001594"
     CDS             complement(5668..6636)
                     /locus_tag="IWK15_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9547184.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6868..7701
                     /locus_tag="IWK15_001595"
     CDS             6868..7701
                     /locus_tag="IWK15_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9547185.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7735..8574
                     /locus_tag="IWK15_001596"
     CDS             7735..8574
                     /locus_tag="IWK15_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9547186.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8600..9160
                     /locus_tag="IWK15_001597"
     CDS             8600..9160
                     /locus_tag="IWK15_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9547187.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9359..11080
                     /locus_tag="IWK15_001598"
     CDS             9359..11080
                     /locus_tag="IWK15_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9547188.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11245..12387
                     /locus_tag="IWK15_001599"
     CDS             11245..12387
                     /locus_tag="IWK15_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9547189.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12403..13614
                     /gene="thiI"
                     /locus_tag="IWK15_001600"
     CDS             12403..13614
                     /gene="thiI"
                     /locus_tag="IWK15_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9547190.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14187..14834
                     /locus_tag="IWK15_001601"
     CDS             14187..14834
                     /locus_tag="IWK15_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9547191.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15227..17872
                     /locus_tag="IWK15_001602"
     CDS             15227..17872
                     /locus_tag="IWK15_001602"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9547192.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18182..19495
                     /locus_tag="IWK15_001603"
     CDS             18182..19495
                     /locus_tag="IWK15_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9547193.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19485..20138
                     /locus_tag="IWK15_001604"
     CDS             19485..20138
                     /locus_tag="IWK15_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9547194.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20193..20744
                     /gene="radC"
                     /locus_tag="IWK15_001605"
                     /pseudo
     CDS             20193..20744
                     /gene="radC"
                     /locus_tag="IWK15_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         7076 a         3485 c         4358 g         6035 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccaagtc
       61 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
      121 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      181 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      241 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      301 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      361 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      421 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      481 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      541 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      601 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      661 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      721 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      781 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      841 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      901 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      961 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
     1021 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1081 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1141 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1201 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1261 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1321 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1381 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1441 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1501 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
     1561 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
     1621 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
     1681 tattgcgtta tttagaagaa attgatatta tattcatgta cttgattccg gcatagctca
     1741 gttggtagta gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag
     1801 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
     1861 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
     1921 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
     1981 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
     2041 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
     2101 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
     2161 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
     2221 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
     2281 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
     2341 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
     2401 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
     2461 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
     2521 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
     2581 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
     2641 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
     2701 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
     2761 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     2821 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     2881 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     2941 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     3001 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     3061 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     3121 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     3181 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     3241 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     3301 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     3361 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     3421 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     3481 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     3541 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     3601 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     3661 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     3721 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     3781 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     3841 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     3901 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     3961 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     4021 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     4081 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     4141 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     4201 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     4261 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     4321 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     4381 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     4441 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     4501 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     4561 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     4621 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     4681 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     4741 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     4801 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     4861 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     4921 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     4981 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     5041 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     5101 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     5161 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     5221 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     5281 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     5341 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     5401 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     5461 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     5521 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     5581 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     5641 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     5701 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     5761 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     5821 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     5881 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     5941 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     6001 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     6061 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     6121 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     6181 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     6241 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     6301 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     6361 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     6421 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     6481 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     6541 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     6601 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     6661 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     6721 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     6781 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     6841 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     6901 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     6961 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     7021 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     7081 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     7141 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     7201 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     7261 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     7321 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     7381 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     7441 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     7501 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     7561 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     7621 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     7681 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     7741 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     7801 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     7861 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     7921 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     7981 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     8041 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     8101 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     8161 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     8221 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     8281 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     8341 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     8401 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     8461 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     8521 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     8581 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     8641 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     8701 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     8761 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     8821 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     8881 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     8941 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     9001 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     9061 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     9121 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     9181 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     9241 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     9301 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     9361 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     9421 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     9481 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     9541 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     9601 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     9661 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     9721 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     9781 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     9841 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     9901 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     9961 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
    10021 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
    10081 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
    10141 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
    10201 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
    10261 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
    10321 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
    10381 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
    10441 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
    10501 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
    10561 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
    10621 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
    10681 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
    10741 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
    10801 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
    10861 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
    10921 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
    10981 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
    11041 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
    11101 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
    11161 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
    11221 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
    11281 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
    11341 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
    11401 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
    11461 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
    11521 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
    11581 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
    11641 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
    11701 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    11761 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    11821 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    11881 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    11941 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    12001 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    12061 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    12121 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    12181 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    12241 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    12301 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    12361 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    12421 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    12481 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    12541 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    12601 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    12661 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    12721 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    12781 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    12841 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    12901 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    12961 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    13021 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    13081 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    13141 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    13201 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    13261 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    13321 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    13381 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    13441 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    13501 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    13561 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    13621 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    13681 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    13741 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    13801 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    13861 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    13921 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgcgaa
    13981 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    14041 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    14101 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    14161 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    14221 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
    14281 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    14341 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    14401 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    14461 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    14521 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    14581 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    14641 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    14701 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    14761 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    14821 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    14881 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    14941 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    15001 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    15061 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    15121 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    15181 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    15241 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    15301 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    15361 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    15421 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    15481 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    15541 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    15601 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    15661 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    15721 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    15781 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    15841 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    15901 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    15961 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    16021 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    16081 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    16141 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    16201 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    16261 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    16321 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    16381 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    16441 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    16501 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    16561 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    16621 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    16681 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    16741 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    16801 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    16861 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    16921 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    16981 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    17041 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    17101 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    17161 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    17221 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    17281 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    17341 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    17401 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    17461 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    17521 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    17581 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    17641 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    17701 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    17761 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    17821 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    17881 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    17941 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    18001 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    18061 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    18121 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    18181 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    18241 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    18301 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    18361 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    18421 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    18481 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    18541 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    18601 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    18661 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    18721 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    18781 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    18841 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    18901 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    18961 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    19021 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    19081 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    19141 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    19201 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    19261 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    19321 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    19381 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    19441 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    19501 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    19561 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    19621 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    19681 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    19741 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    19801 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    19861 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    19921 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    19981 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    20041 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    20101 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    20161 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    20221 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    20281 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    20341 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    20401 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    20461 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    20521 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    20581 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    20641 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    20701 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tttaaccata gtataataaa
    20761 taatagtagt taaagtagtt taagaggaga tattaaaggg atattttact ttaaataaaa
    20821 attaacaagg tttataattt gtagtagtta aagtatttaa agaggtggga acatgtgaag
    20881 gttcaaagga tagaagtgga gaataagccg tatccattgt atttattact agataaagaa
    20941 taccagctaa taga
//
LOCUS       DACDSR010000043        20750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDSR010000043 DACDSR010000000
VERSION     DACDSR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.043"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            342..1031
                     /locus_tag="IWK15_001606"
     CDS             342..1031
                     /locus_tag="IWK15_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547195.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1018..2226
                     /locus_tag="IWK15_001607"
     CDS             1018..2226
                     /locus_tag="IWK15_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9547196.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2520..3791
                     /gene="serS"
                     /locus_tag="IWK15_001608"
     CDS             2520..3791
                     /gene="serS"
                     /locus_tag="IWK15_001608"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9547197.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="IWK15_001609"
     CDS             complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="IWK15_001609"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9547198.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6329..7024)
                     /locus_tag="IWK15_001610"
     CDS             complement(6329..7024)
                     /locus_tag="IWK15_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9547199.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="IWK15_001611"
     CDS             complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="IWK15_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9547200.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8223..8414)
                     /locus_tag="IWK15_001612"
     CDS             complement(8223..8414)
                     /locus_tag="IWK15_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9547201.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8435..9325)
                     /locus_tag="IWK15_001613"
     CDS             complement(8435..9325)
                     /locus_tag="IWK15_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9547202.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9312..10079)
                     /locus_tag="IWK15_001614"
     CDS             complement(9312..10079)
                     /locus_tag="IWK15_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9547203.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="IWK15_001615"
     CDS             complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="IWK15_001615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9547204.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="IWK15_001616"
     CDS             complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="IWK15_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9547205.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="IWK15_001617"
     CDS             complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="IWK15_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9547206.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14695..15033
                     /locus_tag="IWK15_001618"
     CDS             14695..15033
                     /locus_tag="IWK15_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547207.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15201..15959)
                     /locus_tag="IWK15_001619"
     CDS             complement(15201..15959)
                     /locus_tag="IWK15_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9547208.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15977..16795)
                     /locus_tag="IWK15_001620"
     CDS             complement(15977..16795)
                     /locus_tag="IWK15_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9547209.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="IWK15_001621"
     CDS             complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="IWK15_001621"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9547210.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="IWK15_001622"
     CDS             complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="IWK15_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9547211.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17923..19257
                     /gene="dnaA"
                     /locus_tag="IWK15_001623"
     CDS             17923..19257
                     /gene="dnaA"
                     /locus_tag="IWK15_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9547212.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19455..20585
                     /gene="dnaN"
                     /locus_tag="IWK15_001624"
     CDS             19455..20585
                     /gene="dnaN"
                     /locus_tag="IWK15_001624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9547213.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5947 a         4222 c         3807 g         6774 t
ORIGIN      
        1 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
       61 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      121 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      181 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      241 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      301 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      361 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      421 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      481 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      541 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      601 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      661 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      721 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      781 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      841 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      901 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      961 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1021 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1081 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1141 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1201 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1261 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1321 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1381 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1441 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1501 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1561 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1621 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1681 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1741 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1801 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1861 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1921 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1981 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2041 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2101 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2161 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2221 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2281 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2341 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2401 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2461 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2521 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2581 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2641 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2701 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2761 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2821 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2881 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2941 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3001 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3061 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3121 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3181 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3241 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3301 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3361 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3421 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3481 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3541 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3601 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3661 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3721 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3781 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3841 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3901 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3961 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4021 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4081 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4141 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4201 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4261 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4321 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4381 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4441 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4501 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4561 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4621 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4681 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4741 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4801 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4861 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4921 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4981 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5041 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5101 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5161 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5221 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5281 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5341 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5401 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5461 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5521 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5581 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5641 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5701 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5761 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5821 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5881 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5941 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6001 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6061 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6121 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6181 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6241 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6301 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6361 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6421 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6481 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6541 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6601 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6661 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6721 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6781 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6841 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6901 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6961 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7021 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7081 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7141 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7201 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7261 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7321 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7381 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7441 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7501 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7561 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7621 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7681 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7741 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7801 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7861 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7921 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7981 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8041 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8101 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8161 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8221 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8281 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8341 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8401 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8461 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8521 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8581 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8641 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8701 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8761 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8821 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8881 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8941 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9001 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9061 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9121 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9181 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9241 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9301 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9361 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9421 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9481 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9541 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9601 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9661 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9721 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9781 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9841 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9901 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9961 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10021 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10081 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10141 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10201 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10261 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10321 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10381 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10441 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10501 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10561 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10621 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10681 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10741 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10801 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10861 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10921 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10981 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11041 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11101 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11161 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11221 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11281 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11341 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11401 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11461 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11521 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11581 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11641 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11701 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11761 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11821 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11881 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11941 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12001 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12061 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12121 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12181 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12241 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12301 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12361 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12421 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12481 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12541 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12601 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12661 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12721 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12781 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12841 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12901 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12961 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13021 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13081 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13141 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13201 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13261 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13321 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13381 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13441 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13501 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13561 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13621 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13681 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13741 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13801 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13861 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13921 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13981 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14041 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14101 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14161 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14221 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14281 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14341 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14401 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14461 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14521 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14581 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14641 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14701 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14761 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14821 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14881 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14941 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15001 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15061 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15121 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15181 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15241 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15301 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15361 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15421 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15481 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15541 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15601 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15661 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15721 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15781 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15841 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15901 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15961 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16021 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16081 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16141 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16201 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16261 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16321 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16381 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16441 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16501 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16561 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16621 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16681 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16741 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16801 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16861 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16921 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16981 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17041 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17101 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17161 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17221 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17281 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17341 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17401 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17461 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17521 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17581 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17641 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17701 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17761 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17821 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17881 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17941 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18001 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18061 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18121 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18181 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18241 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18301 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18361 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18421 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18481 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18541 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18601 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18661 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18721 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18781 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18841 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18901 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18961 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19021 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19081 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19141 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19201 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19261 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19321 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19381 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19441 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19501 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19561 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19621 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19681 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19741 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19801 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19861 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19921 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19981 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20041 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20101 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20161 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20221 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20281 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20341 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20401 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20461 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20521 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20581 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20641 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20701 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
//
LOCUS       DACDSR010000044        20485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDSR010000044 DACDSR010000000
VERSION     DACDSR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.044"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IWK15_001625"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IWK15_001625"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9547214.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IWK15_001626"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IWK15_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9547215.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IWK15_001627"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IWK15_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9547216.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IWK15_001628"
     CDS             2857..3732
                     /locus_tag="IWK15_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9547217.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWK15_001629"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWK15_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9547218.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IWK15_001630"
     CDS             4629..5063
                     /locus_tag="IWK15_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9547219.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IWK15_001631"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IWK15_001631"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9547220.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IWK15_001632"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IWK15_001632"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9547221.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IWK15_001633"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IWK15_001633"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9547222.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IWK15_001634"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IWK15_001634"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9547223.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IWK15_001635"
     CDS             13334..14260
                     /locus_tag="IWK15_001635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9547224.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="IWK15_001636"
     CDS             14442..15335
                     /locus_tag="IWK15_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9547225.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="IWK15_001637"
     CDS             15469..15594
                     /locus_tag="IWK15_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547226.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="IWK15_001638"
     CDS             complement(15728..16360)
                     /locus_tag="IWK15_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547227.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="IWK15_001639"
     CDS             complement(16508..17050)
                     /locus_tag="IWK15_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9547228.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="IWK15_001640"
     CDS             17185..17574
                     /locus_tag="IWK15_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547229.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="IWK15_001641"
     CDS             17578..18342
                     /locus_tag="IWK15_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9547230.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="IWK15_001642"
     CDS             18471..19418
                     /locus_tag="IWK15_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9547231.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="IWK15_001643"
     CDS             19548..20288
                     /locus_tag="IWK15_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547232.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DACDSR010000045        20398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDSR010000045 DACDSR010000000
VERSION     DACDSR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.045"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..183)
                     /locus_tag="IWK15_001644"
     CDS             complement(<1..183)
                     /locus_tag="IWK15_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547233.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKY"
     gene            complement(368..865)
                     /locus_tag="IWK15_001645"
     CDS             complement(368..865)
                     /locus_tag="IWK15_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9547234.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(995..1705)
                     /gene="budA"
                     /locus_tag="IWK15_001646"
     CDS             complement(995..1705)
                     /gene="budA"
                     /locus_tag="IWK15_001646"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9547235.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1718..3382)
                     /gene="alsS"
                     /locus_tag="IWK15_001647"
     CDS             complement(1718..3382)
                     /gene="alsS"
                     /locus_tag="IWK15_001647"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9547236.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3590..4252)
                     /locus_tag="IWK15_001648"
     CDS             complement(3590..4252)
                     /locus_tag="IWK15_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9547237.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4262..5077)
                     /locus_tag="IWK15_001649"
     CDS             complement(4262..5077)
                     /locus_tag="IWK15_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9547238.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5338..5781)
                     /locus_tag="IWK15_001650"
     CDS             complement(5338..5781)
                     /locus_tag="IWK15_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9547239.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5915..6253)
                     /locus_tag="IWK15_001651"
                     /pseudo
     CDS             complement(5915..6253)
                     /locus_tag="IWK15_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6241..6618)
                     /locus_tag="IWK15_001652"
     CDS             complement(6241..6618)
                     /locus_tag="IWK15_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9547240.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6842..8035
                     /locus_tag="IWK15_001653"
     CDS             6842..8035
                     /locus_tag="IWK15_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9547241.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8201..8623)
                     /locus_tag="IWK15_001654"
     CDS             complement(8201..8623)
                     /locus_tag="IWK15_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9547242.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9206..9700
                     /locus_tag="IWK15_001655"
     CDS             9206..9700
                     /locus_tag="IWK15_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547243.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9862..11034)
                     /locus_tag="IWK15_001656"
     CDS             complement(9862..11034)
                     /locus_tag="IWK15_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9547244.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11240..12055)
                     /locus_tag="IWK15_001657"
     CDS             complement(11240..12055)
                     /locus_tag="IWK15_001657"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9547245.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12205..12651)
                     /locus_tag="IWK15_001658"
     CDS             complement(12205..12651)
                     /locus_tag="IWK15_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9547246.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12648..13652)
                     /locus_tag="IWK15_001659"
     CDS             complement(12648..13652)
                     /locus_tag="IWK15_001659"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9547247.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13692..14021)
                     /locus_tag="IWK15_001660"
     CDS             complement(13692..14021)
                     /locus_tag="IWK15_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9547248.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(14147..16474)
                     /locus_tag="IWK15_001661"
     CDS             complement(14147..16474)
                     /locus_tag="IWK15_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9547249.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(17317..17610)
                     /locus_tag="IWK15_001662"
     CDS             complement(17317..17610)
                     /locus_tag="IWK15_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9547250.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18013..19191)
                     /locus_tag="IWK15_001663"
     CDS             complement(18013..19191)
                     /locus_tag="IWK15_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547251.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(19295..19609)
                     /locus_tag="IWK15_001664"
     CDS             complement(19295..19609)
                     /locus_tag="IWK15_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547252.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(19929..20324)
                     /locus_tag="IWK15_001665"
                     /pseudo
     CDS             complement(19929..20324)
                     /locus_tag="IWK15_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5963 a         4027 c         3476 g         6932 t
ORIGIN      
        1 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
       61 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
      121 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
      181 catatttcat ccatccttaa tgttttttca ttattcaaaa tgataatacc attaaaatat
      241 gattgtgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
      301 aatgaccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
      361 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
      421 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
      481 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
      541 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
      601 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
      661 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
      721 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
      781 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
      841 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
      901 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
      961 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1021 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     1081 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     1141 catgtccgcc aaatgtatgt gtatcatcga taaaatgcag gtgaaatccc gctgcagcaa
     1201 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggata ttgctttgtt
     1261 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     1321 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     1381 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     1441 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     1501 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     1561 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     1621 tatgagcaat cgtttctgtt ccatccatca agccggccat caaagctcct aatgtcccgt
     1681 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     1741 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     1801 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     1861 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     1921 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     1981 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     2041 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     2101 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     2161 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     2221 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     2281 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattttgctt
     2341 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     2401 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     2461 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     2521 agtttcttgc ttcatattcg attggatcat agccaattgc aataactaga tcacttcgtt
     2581 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     2641 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     2701 tttcaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     2761 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     2821 aagcagaacc taatttagga tcgctcaatg gcttgatgac attaccttgg acaggtgcat
     2881 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     2941 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3001 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     3061 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     3121 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     3181 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     3241 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     3301 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     3361 cttgtttcat tttttcagcc attgaacatc gtccttttta tgctttattt ttaattatta
     3421 gttagaaata atcacaaata agtgatacag tttctaaacc tgtttcatac aaaagtatag
     3481 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     3541 tacataaaaa aaactgtcat tcttaattga aagtttcaat ttataaagtc tacagatcca
     3601 tagttctcat caatttacga ttgttgaagt aaatcaaacc aaatacgata gaaggaccta
     3661 gtagaataca ccattggggc tttacgaaaa agaagagaaa caaacttgtc aatagtaaaa
     3721 aatctagtaa actcacatac cattttttca acgtatagta gaacgctatc cgtagaactt
     3781 gatttggttt cgacgctgga tttctcacct gaaaatacca gcagttggtg ctaaaggaaa
     3841 ctgataaaca agcaagtaaa acaaacaacg gaatcaacca ttttccataa gaaaaacgta
     3901 taaaaaagaa acaatcaaaa atcattatca cactaactat ccaagcaaat agccaataaa
     3961 aaaatccctt tagccaaaat tggcgaaaca tctttatgaa caaacgtact ggtgaaaaac
     4021 tattttcttt tgctaattct tctgtcatac ctatcatcac gatgcaacct ggaccaataa
     4081 aaagtaaaga aaaaataaag gaaaagacat tccttccatc tatcgacaaa aggaacgcag
     4141 ctgctatata caagatattg acacaccaaa aacaaaggct acttgccaat gattgataca
     4201 accaataaaa gaattccata taaacatttt tgtcaaaacg ctgctctttc ataatcacct
     4261 cctattcctt cattcctgat gaagcgatcc cttctacaaa atatttttgc atcgctaaaa
     4321 atataaaagc aacaggaaga acagaaacaa ctgaggcagc catgattaat gcgtactctg
     4381 catcaaatag cccaataaac attttcaacc ctaactggat tgttttattt tccgtagaag
     4441 acaaataaat gaatggcccc atataatcgt tccatgtatt aacgaatgtg atgatcgtca
     4501 aactggcaat cgccggtttt gtaagaggta aaatgatctt gcgatagatt ccccactctg
     4561 ataagccatc gatccgcgca ctctcacata aagaatctgg gatactactg taatattgct
     4621 tcaacaagaa cacaccaaaa gcattgaacg cttgtaatag caccaacgac cacaacgtat
     4681 ctgttaaacc cacttttctc atcataatga actgcgggat catataagac tgccaaggga
     4741 cagcaatcgt gccgatatat gctaaaaata aaatatcgcg ccctttaaaa tttagtttag
     4801 aaaacccgta agcagcaaaa ctagaagtaa acaactgaat caacgtgatc acaacgctta
     4861 aaaaagctgt attcttaaaa aatgtcacta aaggaatacg agaccaaata accagataat
     4921 tctctaaatg ccaagtttta gggatccatt gaatcggaat cgtgaacact tcattatttg
     4981 ctttgaatga agcagaaacc atccataaaa aaggaatgat cgtcaataaa gctaatacaa
     5041 tcagcagaat catacccaac cattgaataa agggcatgtt ttttgaacga atttttgctt
     5101 tttttctttc atttcgaaca cctccaaagt tataatcttc atttctttgt aatgattaca
     5161 atccagaaca acggttatta tagctgattc atttccgttc ttcttatttt tgtaagcgct
     5221 tatttaacaa gttatgtaaa tctctttatt tttttgattt aaaaatgaaa aaatacaaaa
     5281 gcaagactgt catacgtctc gcttttgtat tgaaatttct tcgattagag atacttatta
     5341 ctgttcaaaa gcttctttga aacggtgaat caattcattg actacaggtg ataaaatccg
     5401 attctttttc cagactagca tgcagttcgt ttcaatcatt gggtcaagcg gaacgaatgt
     5461 cactcgcttt gaatgatgac ttacagcacc ttctatgact aaagcagcac ctacctgatt
     5521 ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt cctgtgatca gcaactgctc
     5581 cttttttatg cctaaccagt cagcaaacat tcgctgtatt tctggacgct ttgagatcaa
     5641 aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct ttttttgcta aaacagaatt
     5701 ttctaaaatg agcaatcccc atttttcctt tcttggtaac aaaatcgttt cgtatttctc
     5761 tgtatctact ggatctagca aaatcgctaa atctaacagg cttttttcta gtcactccgt
     5821 gatatcatca cttgttccgc tgtggatatg aaattgaacg ctgggataat cacggatcaa
     5881 cttttctagt aatagtgcca atgtattcga actatcagct tccacacagc cgatcgtaaa
     5941 tcggccgctg aataattgtt tttttctgtt ctcgaattct tgagtggttt gattcgttaa
     6001 atctaaaatt tcttctgctc ggctttttta gaaaataacc tgcttctgtc aagatcattt
     6061 tgcggttttc gcgtacaaac agttctgttc ctagttactc ttctaactcc ttcagttgtc
     6121 ggcttaaggt tggttgtgta agatgtagaa cttctgcggc ttgagagatt gttccttctt
     6181 ctgcaattgt ccaaaaatat tttagtaagc gtaactccat agcgatgctc cttgattttt
     6241 ttacttgata cattccattt tatttctaga taacacacga tcatatttct tgatttttcc
     6301 ttgtaaacgt tccaaacatt cttgcatttc agcaattttt ttcgtcaaaa gttcactttc
     6361 ttcaatcaaa agcttacgcc gctcaaatac agttgcttct ccttcgctag ataggcgaat
     6421 atattcaatc aaagactcga tcgataatcc ggcatttcgc atacagatcg taaagtcgac
     6481 ccattttagg tcttcttctg tataatcacg gattccgcct tttgtccgat taactggtgg
     6541 aatcaacccg attcgttcat aatagcgcaa tgtatcggcc gaaatgccct tttcttcact
     6601 gacttgtttg atattcatct gatcactctt tctcttaatt tcttgccact ttacttatgt
     6661 attttattta ttctacaact ttaagtgtac tccaagtcaa tactcatttc tattaaaact
     6721 aagctggatt atctatatcg gttgtttgtg tattatttca aataaaaaag tcttgttttt
     6781 ttattctttt ttggatatac ttttaacaat ttagggaaat tttaggaaaa ggggtataaa
     6841 aatgacaacg tatcagcgaa aggtattagt atcgacatcg gcgggaattg ctttagagaa
     6901 tatggatatt atgtttttag ctttttcatt atcttcaatg attacttctt ttcatattag
     6961 tggtacgcaa gcgggtttca tttcaacgat cactaatttg gggatgctca ttggaggtat
     7021 tttttttggc atcatagctg ataaatatgg acgtgtgaaa gtattttctc aaacggtcct
     7081 tctgttttcg attgcttctt tattgatgta ttttgcttca aacatctact tggtttatct
     7141 ctttcgcttt atcgcaggtg taggagcggg aggagaatat ggggcgtgta tgtccttgat
     7201 ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct tcggttgctg gtattggtgc
     7261 gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg atcattccaa cgtttggttg
     7321 gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg gtcttgttca ttcgtcgcgg
     7381 cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc gaaaagaaaa ccaaacttag
     7441 cagactgttt gaatcaaaac atcttgcttg gcaaacatgt ggccttagta tcatggtaac
     7501 tgtccaaatt gccggctatt ttgggttaat gaattggcta ccttcaatta tgcaaaatcg
     7561 attagggctt tcggtttctg ggtcttcatt gtggatggtc agtactattt taggcatgtc
     7621 tttggggatg atgacttttg gcataatcat ggataaattg gggcctaaac tagccttttc
     7681 tttgttttta atttgttcat ccttatctgt ctatctactt gtgttagccc atagccagtg
     7741 gagtttggta gcagccgctg tgatagtcgg ctactttatc aatggcatgt atggtggata
     7801 tggcgcagtg attagtagtt tgtatccaac agaaatccgc gcgacggcga ataatttcgt
     7861 tatgaacctt ggacgagctg ttggtggttt ttcttctatc gtcatcggtt tcttgatgga
     7921 tcattatagt ttaactgccg taatcttgtt tttgagcggt atttacctag tcagtctgct
     7981 ggtcttgaca actcttactg gtgttacagc attaagggaa agcttgcagc attgatagtt
     8041 gagtctgtaa atgacaagaa agaggtgtgg aaaaagcttg catctgcggt aataagacca
     8101 gccaaatcaa aaacatgaga cactgtttcc gtttggctgg tcgtattact tgatgcaggg
     8161 cagctttttc aactgctaat ttccttctat ctggcggcga ctattctttg acgtccgtca
     8221 tttcaaagta ctttactaat aacttagcat aacgaaaaac tgtggataca ctcgtatatt
     8281 gagcgtctgc ataatcttcc aaatttttct caggatgttg atacatgaat tcaatcaaat
     8341 gataattata tgattttctg tagaaatgaa gataaataat gtagattggg aagttctcct
     8401 gcttttccat agaaaagcgc cgattattct cattcttcaa aatgacctta tcaggatatt
     8461 gttggaacaa ttcttctaaa tctgttagat aattcaagat agacttactt gataccttca
     8521 attcgttttg tagttcttct attgaacacc aacgttctgt ttcaagcagt aaatgaatca
     8581 ttttaatttt cttttgagaa acttttgtta aaaaagtctg catattttct cctcctgtat
     8641 ctactttact atactacata caagaaaaaa acggatacaa atatcatatt gtcacaccgt
     8701 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     8761 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     8821 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca acacgggatt
     8881 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     8941 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagtgaaaaa
     9001 attagttgcg ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
     9061 tactactcaa agcacaagtt cagctcaggc ttccagcagc agtaccacga tctccgaaag
     9121 tacggcagat tcatcgaaag aacaaattga aaagaaaaaa gaagaagaga aacaaaaagc
     9181 ggaaaaagct gcactagaaa aaaaagtgac agaagcggaa tctgctttgt cacaagctga
     9241 agcaaatcct acaaaagaaa actatgcaac tgcttcagca gctatccaag cgctacctga
     9301 aaacaaacaa gagttgacaa gccgtcttgc tgcaattgat tccacaatca aagcaaaaga
     9361 agcagaagaa gaacggaaaa agcaagaaga aattgcacga caacaagcag aacaagcgca
     9421 acaagaagca atcgcacagc aacaagcaga acaggcgagg caagaagcaa tcgcacagca
     9481 acaagcagaa caggcgcgac agcaagcagc agctgaacag gcttctcagc aggcacaagc
     9541 cgcagctcct gagcaaaata atgaacaaac tgtctatgtc acaccgaccg gttcaaaata
     9601 ccatacacat aaatgtggaa atggtacgta tacccctgcc actttaagcg aagctcaagc
     9661 tcgtggttta accccttgct ccaaatgtta tggaaactaa ttcaaaagtt atactataat
     9721 aataaaattc ttctgaaaca taaaaaaata gaagctggcg ttccagatag taaaccaaaa
     9781 atagcagttt acacgtccat ttcgtcagct tctctctttt ttcatttagt ctgttccctc
     9841 tgcttttttg gacatcttca ttcattcttt ctcaatcaaa tagtacacct ctttttcttt
     9901 tcgcagttta tatcttcctt ttttcaattt cattgaaaac tgtttcgaat gtttatgttt
     9961 tacatggatt ttaagcagga gctcctcatg ttgcaagcga tagttcctgc cttttaaatc
    10021 agaaaatctc actagtccgt attcatcaga agctttcaca aatggaactt tttctctagt
    10081 aatatagtgt ttgccgttga gcgtttttac agaaaagacc attccttcga ttggtcgtcc
    10141 gtttgtgttc tttacataaa aagataaagg atagtttcgc ttagcgatgg ccaaagaatc
    10201 taaataagcg atgagtagcc aaactaagcc gattgatcca agtagtatac cgataaacca
    10261 aataaaaaat ttccattttt tccattgatc aattccttgg atcgcttttt ttgcctcttt
    10321 tgcttgataa ggaatacgat gccccgtcac aagcagtcga tgtgtattga ccatataagg
    10381 agtgcaggta agcaaagtca cgagatctct gcctttttgg atacccaaag aatctacttc
    10441 gtcaggtaag accacttgga ttttttctac ttgatacgcc aatgtctttc cattgatctg
    10501 gatgaaaaac tggtctcctt ttttcaactt aggcaaatct gtaaataatt ttgcttctgg
    10561 gagtccacga tgcccagaaa gaacggcatg ggtgtttttc ccgccagtcg gataagaact
    10621 tccttccaat aatgacgttc ctttttgcaa aaatatttcc gttgtctgat cgaatatagg
    10681 taagctgaca ttgatttttg ggatttcgat gactccaagc atatgttcca taaaaaaggc
    10741 attactaggt aaatctttta atgcttttgc atcaactgct tggttgaaac tgcctatccc
    10801 tggtgatacg ttttgctctg ccaactgttg gtttttcttt gtcatttttt cctgctgttt
    10861 ttttatttcc tctgaattct tattcgatgc ttgcttttga taatggatga tcatttgctg
    10921 ggcaagaaaa tcattcagtg aatcttctac aaaaggataa gaaaagatcc cgatcccaac
    10981 gactaaaatg attgccatga agatgtccag tattcttatt ctgcttttat tcatcatgtc
    11041 cacctatttt ctaatatgta tatgaagagg ttgtgaaatc agcgccttga cctgagtatt
    11101 agcgaagcaa acggcaaaat agttcctcct attttgccgt ttgttgagcg aataccaaga
    11161 gaccgaccac ttgtcacaac ctttctcccc atttgaatca ctatcgttta tttatcgtat
    11221 gatttttgtt tccaatagtt taagcttctg tttcttgatg gcgttttttg aaatagatca
    11281 ctgctcctcc aactaaagca gtacctacca agatgaatac gatgatcccc ataccaccag
    11341 ttgaaggcaa gattccttta ggttggttat cgatcgttgt ttcatcagaa gttgttggat
    11401 catcatctac tgtaaattca gtgtacgcag cttcattctt aggtaatgcg tagccttctg
    11461 gtgcttttgt ttcttgaagg cagtatgccc cttgtttcag cccttcgatt gaaaatttcc
    11521 cttctgaatc agaaatcaat ttcacttctt cccagtcaga acctgtgact tcatctgacc
    11581 aagcatatgg gtattctcct gaggcaaggt ttgctatttt tttcattaat ttaacagttg
    11641 gatcttttgc tcttagaata gaaaattctg catcggctaa tttttcttgc gtgttcacat
    11701 cttgtttgat gaagtggaaa tcaccgtagt tttggacatt ttctacagta atcgtgttat
    11761 ccgtagttgt attttcccga ttgactacac cgtttggatt cccaagattg gcttcatctg
    11821 cgccatggat gataccggtt gtttgttgca attttccgtt ttttactata aaagtagttt
    11881 ctttttgtgg attggttaat tgataaccat ctggtgcttt tgtttcaact aaagtataat
    11941 tgccgtctat taatccattg acactgatgg atccttcagt atcagttgtg aatgtcaacg
    12001 ctgactcagc aggatcatag agagcgacac cagtgttttc atcaaaaccg gtcacgaatt
    12061 gattctctga atttttgatc tggaattgcg cccctgctaa cttatctacc cctgaaaagt
    12121 tcccagcttt ctggattttg atattgcttt ctttgatgac atttttagca tataggtgaa
    12181 tcgtgtctaa aggtttgtca gggtctaggg taagcttaac acgatatttg gcgacgtttc
    12241 gacaccgggc tttggtgttt ctacaaagac gtatacagca tctttcccat cagatttacc
    12301 tggaagagtc aggttagcta atccttcacc agaagtcgtt acacttgcga agggtgtagc
    12361 gtctgctgga actgctgtgt ctttacttgg atagtacgta gcagtgatgt catatacgtt
    12421 gaattcaacg ccgttcaacg gttcgctatt cggaaatgtc aattccgttc cgttgtttat
    12481 ttgattttct ggtgctgaag tgaatttcaa tttatgcaaa ttaaccgtaa cattttctgg
    12541 tgtaggtgat acggctgcag aaacctctgc agttcctagg ccgagaacga aaggaagcag
    12601 catgatagca gttcctaaaa acgtccatat ttttgttttg atgtttttca ttttatttcc
    12661 ctcctattgt ttcctttttg ttgtttttta taaaaccaag ctgtccctgt tgaaaggact
    12721 agcaataacc ctaaaattga gaaacctcca agcatctctt cgcttgtttt tggaaagctt
    12781 cctccatttt tttcatttga tggaccattt gtctgcatct gaactggcgt ttttccatta
    12841 ttcgtcggat ttgcttttgg ttcgttgatg atttccaatg gtataggatg aagcggatca
    12901 taactgcctt tttcaacttc aaagagaatg gttgattgac tcaatatata tcctttaggc
    12961 ggtttaatct ccactagttc gtaacttcca aaagacaatc catggacaga aaatgttcca
    13021 tctttactgg aagtaaaaat cgttgctctt gaatcatctt tggctaccca atgatttatc
    13081 ccgttttctt gcgctaagta ttggtcattt tttttgacta gaaattgagc atctgctagt
    13141 ttttgtttcg tttgccgatc cactttgaca aattgtttgc ctcctgtttg gatggcaaga
    13201 tgtgtttcta ctgtttgatt gtcagtcgtc aaacgtacat cgttatcaaa tgtcgttttt
    13261 tcactgtttt ctttcaatgt catttggtaa cggatcgtta atttttttcc agcaaacgaa
    13321 ctaagatctt ttccagaatc tgataagaca aaaccatgtt ttattttttg tgtgtgaaat
    13381 gtatgaatct tgtctccacc aatcaaaaaa tcgatcgttt ctggttctag ccataactga
    13441 ggatcagctg tatctttgat cgtataggtt cctatctcgt tgatgttctc aggaattgtt
    13501 acggtgatga gataagggac tttatcccca tctgcaaaat ctgtctgctt attctctact
    13561 gtttttgtta atgtgagtgc agcaggtatt ttttgttcgc tctttggata caagtggatg
    13621 gtcgtcaatt tatgattaga tgaatctgtc acaggaagca caacgacaaa tggtgctgat
    13681 ttttctttta cttaatctga agtctttatt tccgtaaatc ggtaaacagc gtcacgacct
    13741 tgctcatctt tgtccgatgc actaaaagaa gcaatgccct cttcctcgtc tactgttttt
    13801 gtcacatttt ctgccagtat tttttcacta tcactttttt gtgctagttt tctttgtgcg
    13861 tcttccacac tacttccttc tgaccgcagc ttgtaaaatt catccgagac atcatatata
    13921 gcgaaagtaa cgccgttcaa cccttttact ccttgaaagt tatccgggat atttattccg
    13981 tcgttttgac tgatacttgg catcttatcg ctttcgaaca ttcgattatg caaagtaaat
    14041 gtaataggtg attctctagc tgacacagtc ggagaaccta taaaaaagac cagtaggata
    14101 aaaaagagaa cagcaaatat atccacacgt acattttttg tttttttcat cgctgttcac
    14161 ctgctttttt cttacgatgg ataaaataaa agaagccaga aaaaataaag atcagtccta
    14221 aaaagacaaa gggcagtaga cctatcccac ctgtcgaagg aagcaagaga gtcggattga
    14281 tcgtcaactt agcggtatct gttgcgataa tcgtataatt attgtttaca cttccttcgc
    14341 ctaaactaat cgttactatt tgtgatccaa tatctgtccg gctgttaact gttagggtat
    14401 aatccaatga ttcgcctaag acaacaccgg taacgtggag atttccaacc gaaatcgagt
    14461 gactttgggc atcataatta aaggcagcgt catcgacagt aatcactgcg ttagctttat
    14521 agatggtata agttcctgat gtgttggcta tatcagtaaa tgacaacttg ggatttgctg
    14581 ctttgacttt gtctattccg ctattcgtta gtttaattgt atagctgcca gcattaacca
    14641 tattttttgc tgattgtgca tctgctgtgt aatccatact gctaagtgcc acttgtactg
    14701 gagtaagcga actagtgaca ccctctgcta gcttgacatg gattgacggg acacctgctt
    14761 ttccatcata tgtcttactg ccactgagct ctaccgcacc gctgatttgc ttaatcgtat
    14821 agacatcaga tgtctgcacg atagtaaata aatagtttgt tctggcagct agtttagcta
    14881 gtccgctttt tgtcaattgc caagacgtat aggttccaat ttcagtaggc gcagttactt
    14941 caactccctt ttcattcatc gctttgaaat cagttggatt ccatgtgatt tgatcaatac
    15001 tgagatcatt gtttaaaagg gatgttgatt ctgttttatc attaaaggca tatttaacga
    15061 tcggtacaac agaaagacca tcatagtact tactgccatt tgcattgatt gatactgttg
    15121 catttgtctt ataatatagg atgacttcag aaatctgttt tccatttgcg tcctttacga
    15181 ttacatttcc atttgtatct aaactagcta aaacactcat tgctgattgg ttctttgttt
    15241 ctatcgctga aacagctgta aaggtatatc cgttgatagt aggcgtatta gtcgtcaggt
    15301 cttgtgcagc acttccgtaa gtaagattca ttattttaga aggagcaatc atctgtccaa
    15361 aatagtcttg atacttaata gtgattggtg tttgtttgcc tgtataggtc accgtatcga
    15421 cgttactttc tagggctgtg aaagaaccgt ttgtttgtgc aaaagaatag cccgttactg
    15481 ctgttggagt gataaccact ttgtcaccca catggattcc agataatgta gtcccgtacg
    15541 ttccgatttt tgctgctcca ccattgactg tataagaaaa agttcctgtc cgattcgcat
    15601 tgacctgaac cgttgctgct tgataggttg gaaaatttga aacatttccc ttaccatcaa
    15661 cgatctgccc atttttgatc gagtaccccg tgaatggaat atcatttgct aactgtatac
    15721 cgattgaaaa agtactcata cctcctccgg caggaagccc tccccatgtt gagggtgtaa
    15781 aagaataact tttatccgca ttgggtgtaa ctgaatcgat cgtatttcca gcggtagcca
    15841 cccccatata gatacttttt gggaatgtga tagtagtacc atttactgtg gagccaataa
    15901 acagattatg gatatctgga cggttatcaa taaaagcgat tgctttggtt acgccttgta
    15961 acgcagtttt atccgcactc atcgatccgt taaccgcaac aatgactcca tctttggcat
    16021 atatcactgt atatccatct ggtatggtat gtccgttgaa attcgcagtg gccgcaacag
    16081 tactaaatgt ctggcttcta cctgtttctg aataagcttt ctcccctgct gcttttgctg
    16141 ctgtgatttc tttttctgtc ggatcaatca gttgagtcga ggatgtggtt gaggttgtct
    16201 cgttgttgct agactgattg gtgctttcta tggactgtat ttcagaatag ggtggactat
    16261 ttgtctcctc tgtcgtttgg ttagcaaaat ttgacgtttc tgtataaacg tttacataaa
    16321 tacttgataa gctccctaag aaagtaaaaa tcaagatgat cgaaatcata atgggatgta
    16381 agtattttct tttagataca gctttgagtc tagaaacaaa atgtttttga catatacgtt
    16441 ggattttatt ttgagttttc ttaatcattt tcatccctcc tctcaataaa aaatcatcaa
    16501 ataatccttt acacgttatt ctaaatttaa tttttattat tttattgatg tatagaaagt
    16561 ttggggcttt ttttctcttt ttattctaag gttattttag tcttgtcctt taatacaaag
    16621 attttatttt taaataaaaa atactctttt tttacgatta ttgaaaaaaa aagataaaac
    16681 ttgattaatt gaagtattgc ttaaatttgg aagctatgag ggaagaataa aagagttagg
    16741 acgggattat ctactatttt tctttgtttt aaatatttgt tatctttgaa cataaaagag
    16801 aaatgtgtcg actgcagtgt tgtgtccctc atcacgaata aacggtgttc aattagctga
    16861 tcttgtattc acttaaaaaa tggtaaagta cgagttgcta ttccccattt tcaattttct
    16921 aacgctcagg acaaaatact gttttcacaa cctcattgga ataaaagcat atatattgtt
    16981 tcaagaacga aatcttgtta tctttttaaa gggcatctgt ccaaaatcta attttaggag
    17041 gtacgttttg gtatttattc gtacaaataa aagagaatta tctttattca tgtaacgcat
    17101 ccaattgatt ttttcatgta cgctcctgga acgattttac tgacgattgc ttatttaact
    17161 gcgaatcgct cattagcttt tatcatagct atccatattt tgaataacgt tcttggcttt
    17221 gtattataga tacagatgac aaaaaaagtt caagtatgcg gtgtagaacc aatatcgcat
    17281 acttgaactt ttttcaatga gtataaatta aaacttctaa ttttcaattc ccatcatctg
    17341 atatgcccga ccgtctgaac ttcttttgaa aaagccaaat gtgattagat aacgtctttg
    17401 ttcgacataa tcgccactag aaaatacgtt cagcagtatc gaattaacct ctgtttctgt
    17461 ataagtcact ccatggtcaa agcgtttcaa gacttctttc aataggatgt atttcttttt
    17521 tgttgaactt ggtacttgtt ttacttttcc atcattcatg aatgtgtcta aaactttcat
    17581 cttttcttgc tccgtataag tataaaccat cttctttctc cttttgattc taagaatact
    17641 ccatttatgt tagaatattc tggatttttt attaaagcgg ttactcttaa gaaagatatt
    17701 atcatagtgc tctttttttt actactactt ataggtagat ttattctaat ccattattct
    17761 cccctaccaa taattaaaga aaaaactgga tcagggtgtt caaattacat gctaagaaaa
    17821 aagaaccctt gaataccaag ggttctttct cagtctgata acaatgatta acgacggatt
    17881 tctttgatac ttgtgaaaag tcgttaaagt attgatataa aaggattttt attacctctg
    17941 actacatatt gactaatttg ttaaaaaatc gtttttcaag aatcctgtaa taccagtgct
    18001 ttcaccgttt ttttaactct ctaaagaact aaaagcaatc agaggtttta ccttttcatt
    18061 tgacgttaag tggcgataaa ttttcgaagt tgtttggtca gtatggccta agcgatcttt
    18121 caagacttcc cagctacagt gttccgctac taagcttgcc attgtatgac gagctaaatg
    18181 agtatgaaat ggcttattaa ttcctgagcg attcacgcac cgtttgacgt gatcatttac
    18241 atactctcga cgataaggaa caccacgttt actagaatca agatacacaa acaaatatgt
    18301 ttcttctata ataaacctgt aatgttgcct catctcaccc aaagccttaa tcaactgttg
    18361 tagcttttcc ataactagag gagttactag aactcgtcta gcagaactaa ctgtcttagt
    18421 agtttcaatt cgaaaatcct tactcttacc tttaactgcc gtttgcttat agaacagaat
    18481 ttcatttctt ttgaaatcta tatctccagg ctgtagagca agcgcctcgc tgactcggca
    18541 gccagtgtaa aatagaacat ggaaaagtac agacatcggt aattcctcat gaacaatgcc
    18601 ataattcaaa aaagttctca attcatcgac agtaagaaac ttgtcctcaa tattatttat
    18661 ggcttctttt aaatcagtta ccgttaactg atattttggc attctcacag acttcattgg
    18721 attttcactt atcaaattct ttctttgaca atatttaaaa atcatattta gaacttgaat
    18781 aagtgattgt aaactatttt tggcatagcc aacatttgtt ccattaacgc cacattcctt
    18841 taaggtaaaa agcaattctt gaaggcactc tggtgtcaaa tcagacaact ttttatcacc
    18901 aatccatcga acaataattt ttaattgttc ctctcgctta tcataggtta tttccttttg
    18961 ttcatttaac gaacggtaat attggaacca ttgctcacct acaacactaa acttgtcatt
    19021 gcttgcaaca atttttttct taagcctttg ttgtttctcc atttccagat aagcgagttg
    19081 cgcctcacgt tttgaattaa agccacgtct tgttgttcga tatgcttgaa agtaccatgt
    19141 gatcctacca gtagaactga tttgcttctt gaatcgatct gttttttcca tttattgatt
    19201 cctcctattt tgaaggaacc caacaagcag aaaccctttg gaaatctttc tatctatgtc
    19261 atcattgtac cgcatcttag aaagaaaatg taacttataa agatcgaagt agtcgcaatt
    19321 tttctcgata gtcattcgaa tcactctttc tcatccattc taaaacatca tcacgaaaat
    19381 agtgtcctcg aatcccgtac caacagggag gaaactcacc aagttttact aaacggttca
    19441 gtgtactatc acttatctga aatagtcgct ttaaatcagt atttgttaat accagaggta
    19501 aatgactgac tgcaagcaat ttttccgtgt aatcttccag ttttttttgt aaaattgaat
    19561 agaactctat ttccagagaa ttaacatttg ttttaacttc caactccatc acccttctct
    19621 ctcaaaatca aaacgaccac cacggtaccc cttaaaattg taaaaaaaca ctccagtact
    19681 ccatcaaaac ggactagaaa agaggaattt ttaatatgaa acaaggaatt gcagacataa
    19741 aaataattaa agaaattctt gaaaaatcta cggctaatgc aatagcattc ggaacaggaa
    19801 tcaacttgag tacagtaaaa aaacttaaaa gtggagagag agctgaagaa aaattaaatc
    19861 ttgccgatgc tattaaaatc acagaatttg gcatgaaaaa tatgccaacc aaaatcgaaa
    19921 tatggaaatc ctagctcttc caaagccatt tgtcgttggt accaaacact gccgagactt
    19981 ttcccaataa tatcgccaat ttcacgatca ttaagaccga tgaagaagga cataaaaatg
    20041 atttcttgtc tttgactagg taatgctgca atcgcatttg ccaactcctc attggaaata
    20101 aagattttca taccaaggac aataaaagta ccactactat tcaaatttgg ttccaaataa
    20161 tagaactctg tacctaaccc tttttgttcc aatatttcca ttgatatttg ccgttggcga
    20221 aatctagcgt acttcttttg aatatttaac gcctcattgc agactacttt cttacaaaaa
    20281 ctgtcaaacg tatgttgaat gtgggtttcc tctgagtcgc ccataatatt ctccccctca
    20341 aaaaattcat tcaactcgtt ttacttctat ccctttccat tattactaca atctagga
//
LOCUS       DACDSR010000046        20279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDSR010000046 DACDSR010000000
VERSION     DACDSR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.046"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            93..557
                     /locus_tag="IWK15_001666"
     CDS             93..557
                     /locus_tag="IWK15_001666"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9547253.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(619..1662)
                     /locus_tag="IWK15_001667"
     CDS             complement(619..1662)
                     /locus_tag="IWK15_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9547254.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(2039..3004)
                     /locus_tag="IWK15_001668"
     CDS             complement(2039..3004)
                     /locus_tag="IWK15_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9547255.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            3238..4083
                     /locus_tag="IWK15_001669"
     CDS             3238..4083
                     /locus_tag="IWK15_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9547256.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            4274..5461
                     /locus_tag="IWK15_001670"
     CDS             4274..5461
                     /locus_tag="IWK15_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9547257.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            5467..5823
                     /locus_tag="IWK15_001671"
     CDS             5467..5823
                     /locus_tag="IWK15_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9547258.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5825..6163
                     /locus_tag="IWK15_001672"
     CDS             5825..6163
                     /locus_tag="IWK15_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9547259.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            6558..7592
                     /locus_tag="IWK15_001673"
     CDS             6558..7592
                     /locus_tag="IWK15_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9547260.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7585..8271
                     /locus_tag="IWK15_001674"
     CDS             7585..8271
                     /locus_tag="IWK15_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547261.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            8295..9143
                     /locus_tag="IWK15_001675"
     CDS             8295..9143
                     /locus_tag="IWK15_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9547262.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            9340..10113
                     /gene="sufC"
                     /locus_tag="IWK15_001676"
     CDS             9340..10113
                     /gene="sufC"
                     /locus_tag="IWK15_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9547263.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            10126..11412
                     /gene="sufD"
                     /locus_tag="IWK15_001677"
     CDS             10126..11412
                     /gene="sufD"
                     /locus_tag="IWK15_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9547264.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            11409..12644
                     /locus_tag="IWK15_001678"
     CDS             11409..12644
                     /locus_tag="IWK15_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9547265.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12631..13101
                     /locus_tag="IWK15_001679"
     CDS             12631..13101
                     /locus_tag="IWK15_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9547266.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            13106..14497
                     /gene="sufB"
                     /locus_tag="IWK15_001680"
     CDS             13106..14497
                     /gene="sufB"
                     /locus_tag="IWK15_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9547267.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14592..15734)
                     /locus_tag="IWK15_001681"
     CDS             complement(14592..15734)
                     /locus_tag="IWK15_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547268.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(15773..16507)
                     /locus_tag="IWK15_001682"
     CDS             complement(15773..16507)
                     /locus_tag="IWK15_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9547269.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            16739..17014
                     /locus_tag="IWK15_001683"
     CDS             16739..17014
                     /locus_tag="IWK15_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547270.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17112..19109
                     /locus_tag="IWK15_001684"
     CDS             17112..19109
                     /locus_tag="IWK15_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547271.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(19185..19520)
                     /locus_tag="IWK15_001685"
     CDS             complement(19185..19520)
                     /locus_tag="IWK15_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9547272.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            19647..19862
                     /locus_tag="IWK15_001686"
     CDS             19647..19862
                     /locus_tag="IWK15_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9547273.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19899..20261
                     /locus_tag="IWK15_001687"
     CDS             19899..20261
                     /locus_tag="IWK15_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9547274.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
BASE COUNT         6714 a         3567 c         4226 g         5772 t
ORIGIN      
        1 caaactttta agaacatttt ttattagttg gcaagtagtt ttggatagag aaaaaggagg
       61 aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct atcggctttt
      121 tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca atttcagaaa
      181 gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa gaatattttg
      241 cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg ccttttcagc
      301 aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg tataaagaag
      361 tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca aatcgaacca
      421 atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga aaactgacag
      481 gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg ttgttagaag
      541 gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga gaatcacaac
      601 tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag ccatcactgt
      661 ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt tcatgaattt
      721 tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc agctgctgat
      781 aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga ccaaacaaaa
      841 gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct gcgtatcagt
      901 tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca gatagacaac
      961 aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga caacagaatt
     1021 gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac cacccaaaag
     1081 tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag ggagagtcaa
     1141 catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt cttcctcttt
     1201 cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag cgtttcctcc
     1261 agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca agtaagcacc
     1321 gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac caatttgagc
     1381 aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta atagtttgct
     1441 ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat ttcctaataa
     1501 aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa tagcaagaag
     1561 cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt cgcggaccgc
     1621 tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg ttcctttcgt
     1681 caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt tgacaaaaga
     1741 acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaataaacg gtgttccaga
     1801 agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
     1861 ttccttctta tttcttgaag ctgaccactt cttttacgac ctcttggtaa acggtgttca
     1921 aaagcttgca tctgcggtaa taagcccaac cagtacaaaa acatgaacag ctgtttttgt
     1981 ttggccgttc ttattacttg atgcagaaca gctttttcac aacctattga atcgattatt
     2041 aagcgatttt tttagaaaca aattttccat gtttgctatg ttctttaatt aagaaacgta
     2101 cgtaatgagc aagaacgtag aagacaacta cacttgcaaa cagacattcc gcaactttta
     2161 agaaatccaa tacgctacta tagataccta aactgttgaa gtaatgaaca gtagtaaata
     2221 tagctgttgc agagataaca agcgggaatg taaaggctgc atagcttgga taaaaacgaa
     2281 gcttgaacaa acgtggaagc atagacagca caacgaaata caatacttga gataaagcga
     2341 acaatgctac gacaagataa aaattaggtt cagcaaagtt tttaagataa cctgttaggc
     2401 aaagagaacc aggtgctgca acgatcgtaa tcagcggacg tgtcggatga gccatatttc
     2461 ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa cgcatagcag atcaatgaaa
     2521 tccaaaagaa aatttttcca ataagtggga agaagttccc ggcagttaca gtgatgaccc
     2581 caaatccaac gtaaatgata aaccagcttg gataaattgc ttcaattgaa agatcagctt
     2641 tgataacatg gtgataattg aaataaaaga caaggatcac ttggaagata acagcgcaca
     2701 gccaaatgaa tttgacaatt ggtgcaatac tttcatatga aacaaaataa gtacaaataa
     2761 ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat gattggatca tgcaacgttt
     2821 gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat gaatatcata agaatcatag
     2881 agataccgcc agttagattg cctaaagcaa catggtgata gtctttaaac agatttccaa
     2941 gggaagctaa cccaaggatc aacccgcaga tcgggatcgg aataattttc aaaaagttct
     3001 tcatatataa tattctcctt tttatttaat agattttagt accaagatga acgaatacat
     3061 tgtaaaatga ataggaatgt atgattacga gcaagcagac agctgattgt cttcatctaa
     3121 gttactagtt ggatcagcaa ctgttttgtt gtacatttct attatagatg gttttttgta
     3181 tatttaaatc aaatagtttt tatagtaatt atcataaaaa gttatggaag tggtgccatg
     3241 tttaagttat taacgacttt tcgagcagta tatgaaacaa gaaatttttc cagagcagca
     3301 gaagtactgt ttttatctca accagcagtc tcgaatcaaa tcaaacagtt ggagcgggaa
     3361 ttgaatatcg atctttttca acgtaacgga agaaaagaga tgcttccaac taagcaagcc
     3421 gacttattat atgaacggac actggttttg ttagaagaat gggaagaaac tcagcaaaaa
     3481 ttgctcaatg aacaaaatga agaagaagta tgtcggatcg ttgcttccca cacctttgct
     3541 gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa ggtttcctaa agtgcatttt
     3601 gagatcgaaa tcacgaattc tcatgaatca ttagaacaag tagcgaaaca tgaatccgat
     3661 tttggtttta tcgaaaagcc attagagact tccggcgtac aaagatactc tttgatggaa
     3721 gaccagctag tcatcgctgg ggacccaaaa agcaaattgt ggcttgtaag agaagcaaac
     3781 tctggtgttt ttcattacac gcagcgttat tttgaagaga tgaatatcca agataagaag
     3841 atgatcgtga aaagtaacga tgtaattgtt gcgttgctga aggaaggaat cggtcgctcg
     3901 attttgtcta agcgctctgt tccagaaaat gtgccaatga ctccgttgaa tttccatcga
     3961 tatttttatt tcatccaacg aaccaatctg gtttctgatc agctgctaga gattgccgaa
     4021 gagatccttc gatacaatga aagcacagaa acttctcaca gcgaggagaa tctcagctgg
     4081 tagataattg catgtatcaa aaaaagttct atgatacact aaagataagc agtaggcttt
     4141 tgtactgcat ttgctcaagc aactgcacaa acggaaagtt aatttttccc aaatgtctat
     4201 ggaaagactt tccattatct aactggaccg ctataataat cagcagtatg gtttttaaga
     4261 aaagggatga aacatggaaa aaaaattaaa agcttcgaac gataatcgta ttgattatgg
     4321 cgtgatttta ccagtctttc tgttatgttt gattggtctg ctctcattat atgtagcact
     4381 tagccatgat ccgaacaatc cagcagtctt gaaaggcgta gggatgcaag ttgtctggta
     4441 tttagttgga gcggcagcaa tcgttgtcat catgcatttg aattccaaat ggatctggaa
     4501 actgactcct tacctttatg gtttggggtt agtggtaatg ggactcttgc tcaaattcta
     4561 tgatcagaat ttagctgctt caacagggtc taaaaactgg tttcgattcg gtgcgtttac
     4621 tttccagcca gcagagttga tgaagattgc gtacatcttg atgatggcac tgatcgtaac
     4681 taaacataat acgcaagtaa aagaacggac gatgaaatcg gacttttggc tgattgggaa
     4741 gctcttgatc gtgacaatac cggttttagc attgatcatg gcccaagatg actttggaac
     4801 gatgcttgtc tttcttgcga tctttggcgg gattttcctg atgtctggaa tctcttggcg
     4861 catcatcgca cctgtagtaa tcttggcagt agtcgttgga gcaggaacga tcttcttggt
     4921 tacgactgaa ggcggaagag acttgcttta taaagttgga ttcaaatctt atcagtttgc
     4981 acgtattgat tcatggctgg accctttcca tgatacgtca ggaatgagtt atcagccggc
     5041 ccaaggactt ttggcaattg gtaccggtgg cttgtttggt aaaggattca acgtaagcaa
     5101 tatctatgtg cctgttcgtg agtccgatat gatttttacc gttatcggtg aaaacttcgg
     5161 ctttatcggt ggtgcattcg tcattttctt gtattttatt ttaatctatc gtatgattcg
     5221 ggtgtgtttc gatacgaata atgaatttta cgcctatatc gcaagcggtt tgatcatgat
     5281 gctgctgttc catgtctttg agaatattgg agcaaatatc ggattacttc ctttaacagg
     5341 gatcccattg cctttcatta gtcagggagg atcctcgatt ttagggaaca tgatcggtat
     5401 cgggttgata ttatcgatga gataccaaaa tgaagcacct gcaagatctt caaggaggta
     5461 agaaaattgt acacctttta ctggtacccg aaatgttcaa catgcaaaaa agccaaagcg
     5521 tggttagacc agcatggcgt tagttatcaa accgttgata tgatcaaaga agtaccgaag
     5581 gcctcaacga ttgagacttg gctagaaacg agtcaagtac caatccgtcg cctgttcaac
     5641 acaagcggga tgaaatatcg ggaacttgga ctgaaagatc aagtaggcga gtttacggct
     5701 tcaaaagcga gccaagtctt atcgacagat ggtatgctga tcaagcgacc actgatctta
     5761 cgagaggacc agcttgttgc aattggattc aatgaaaaaa tttatgaagg agaactgata
     5821 taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt tggatcttat ttaatggaaa
     5881 agaatactgt gttggtttga caaatgaagc gcaagaagaa cttggagaca tcacgtttgc
     5941 caatctgcca aaagcaggac aatcgctaaa aaaagatgaa cctctgatcg aagttgaagc
     6001 ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt acagtttctt ctgttaatga
     6061 taaaatcggt gaagatatca atatcttgaa tgattcagat gaaatgaatg cgtggatctt
     6121 aagtttcaaa gatgttgatc caaaagaatt tgacgaatta taaaatcaga aaatcacttg
     6181 aaaaaagttg acactgattc ttccagttgc tataatcata agcaatccta tatgaaacga
     6241 gaaagctttg aaaagaagag tacatgtcac ggacgtttga agagagccct gtttgctgaa
     6301 aatgggtaac gaaaatgaga tggaagatgg tcttagagca gagtaagcga gcgtaagcaa
     6361 acttactacg ggagtgcccg ttacagcgcg acagtatacg gaaagaagct aagtccaagt
     6421 actggaagag gctgttattg cgaagtaacg gtaaatcaag gtggtaacac ggaagttcaa
     6481 gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt tttgtttgaa aaatcataag
     6541 agaaagatgt gaatggcatg tcattaatcc aactaacgaa tgtcaaaaag acattcaatg
     6601 ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact aaacgttgaa aaaggggata
     6661 tttatggaat cgtcggctat tctggcgcag gaaaaagtac attagtccgt ctcctgaatg
     6721 gacttgagct tcccacgagc ggcgaagtga tcgtaaacga tcaagatatt acaaaattaa
     6781 aaaacaaaga attacgtaca ttccgaaaga aaatcggtat gatcttccag catttcaacc
     6841 tgttatggtc aagaactgta cttgaaaata tccagctgcc gctggaatta gctggcgtac
     6901 caaagagcaa acgtaaagaa cgggcagaag aactgttgcg tttagtcggg ttagaaggac
     6961 gcggacaagc atatccttca caactttccg gcggacagaa acaacgcgtc ggtatcgcac
     7021 gagcattggc aaatgatcct gagatcctgc tttgtgatga ggctactagc gcattggacc
     7081 cgcaaacaac agaagaagta ttagacttat tgttagcgat caataagaaa ttgaatttga
     7141 ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa aatctgcaac aaagtcgctg
     7201 tgatggagtt ggggaaagtc gtagaagaag gagacgtatt gactgtcttc agacaaccga
     7261 aaaaagaagt aaccaaacgc tttgtacaac aagacatcga accagaagct gattcgacag
     7321 aactattagc tgaactcatc aaagaaaatc caagcggccg tttggcaact ttgaccttta
     7381 gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat tcgacgctat aacatcgatg
     7441 tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg ttcctttggt tcattgactg
     7501 tcttgatgac tggtgaaacc aaagaattag atcaagcaga agtgttcatc aaagaacaag
     7561 gagtaggaat tgaggtgatc catcgtggat aaaagctttt cagaaacata tctagatttt
     7621 aaacaaatca acccggaaac gatgaatcag gctatccttg atacactata tatgacatta
     7681 atctcaatgg tcttagtgac gatcatcgga ttagttttgg gattgatcct ttactctatt
     7741 ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa tcgtatcgat catcagtaat
     7801 attttccggt caacgccatt tatgatcctg atggttttga tcattccatt tactaaagca
     7861 atcgtaggca ctatgctagg tgcgcaagca gccattccag ctcttgtttt atccgcagca
     7921 ccattttatg cccgtctagt agagattgct tttcgtgaag tcagccctgg tgtactggaa
     7981 gcagcagatg caatgggagc aagttactgg gaaatcatct ggaaggtctt gatacctgaa
     8041 agtattcccg cattgatttc tggattaaca gttacaacga tctcgatgat cggatttact
     8101 gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc ttgcttggca ggaaggctac
     8161 cagcgtggta atctaacagt gacattcgtt gcgactttaa ttattttact aatcgtattt
     8221 atcgttcaag gaatcggtga tttcttaact aagaaaacag acaaaagata ataggatata
     8281 gggggatgta ggatatgaaa aagaaaattt ttggatttgc agcagcgctt ttgctgactg
     8341 taggattagc agcatgcggt aacaacagcg acagcaaaga ttcagcaaat aaagcagacg
     8401 acacgacatt gaaagtcggc gcttcaccga caccgcacgc agagattttg gaacatgtga
     8461 aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt caaattcgat gactatgtat
     8521 taccgaacca atctttggaa gaaggcgaca ttgatgccaa ttacttccag catattcctt
     8581 atttgaacaa agaaatcaaa gaaaaaggat atgactttgt caatgccggt gctgtccaca
     8641 tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt ttccgaatta aaagatggtg
     8701 caacaatcat tacttcaaat tctgaatcag actggggacg catcatcaca attctacaag
     8761 atgctggctt agtcaaagtc aaagatggag tagatctgga aacagcaaca ttcgatgata
     8821 ttgaagaaaa ccctaagaac ttgaagttca accatacgat cgacccatca cttttagctt
     8881 ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa cgcaaacttc gcttacggcg
     8941 cagggttgaa tccattagaa gatgccgtat tgcttgaaaa ggacaactct ccttacgtga
     9001 atattattgc gactcgtaaa ggtgatgaag acagtgacaa aatcaaaaaa ctgattgaag
     9061 tgcttcaccg caaagaagtc caagaatgga tcacagacaa atggggcggc tctgttaaac
     9121 cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag atgagaaata agtctgtaat
     9181 ggcttatttc tcatcttttt tatacctatt ctaatttaga aatcaaaatt gtttaataat
     9241 aaataaaaga tatttgattt tagtaaggaa ggtcattgcg cttcattctc aattagtcta
     9301 aaatgagtga gaacgaaaga gaatttgggg gtataatgca tgtctgtctt agaaataaaa
     9361 aatctacatg tgtcgatcga ggaaaaagaa atccttaaag gcgtaaacct aacaatgaaa
     9421 accggagaaa tccatgccat catgggacct aacggcacag gaaaatctac cttatctgcc
     9481 gctatcatgg gcaatccaaa ctatgaagta acagaaggcg aaattttgtt tgacgggaaa
     9541 aatgttttag acttggaagt agatgaacgt gcgcgacttg gcttattttt agctatgcaa
     9601 tatccaagtg agataccagg tatcacgaat gcggaattca tgcgtgcagc gatcaatgcg
     9661 aaacgtgatg aaaacgacaa aatttctgtt atggcatttt tgaaaaagtt agatgaaaaa
     9721 atggctttac taaatatgcc agaagaaatg gctgaacgtt atctgaacga aggtttttct
     9781 ggcggagaaa agaaacggaa tgagatcctg caattgttga tgttagaacc tacatttgca
     9841 attttagacg aaatcgattc aggtctggat atcgatgctt tgaaagtcgt tgctaaagga
     9901 gtaaatgaaa tgcgcggaga caatttcggt gcattgatca tcacccacta tcaacgatta
     9961 ttgaactata tcacacctga tgtcgttcac atcatgatgg aaggccgagt agtcatgaca
    10021 gggaatgctg acctagctaa acgtctggaa gcagaaggct acgctggtat cagtaaagaa
    10081 ttaggaatcg actataaaga agaagaagcg taagggggag caagaatgaa agaaaaaaac
    10141 tggctcgatt atcttgacgc ggtaaatgat ttttctttaa gtaaaggcga acctgattgg
    10201 atgagaacct tccgtcaaga cgcactagcg aaagcagatg aactgccatt gccgcatatc
    10261 gatcgggtga aattccatcg ctggtcgtta tttgatgtga aagaaacaca aaccatcagt
    10321 gaaacaggca cgattccggc atttgatgca atgaaagaca atcctgtgct tgtccaacaa
    10381 ggttcatgga cgatttttga acaactgcct gtggaattag cagaaaaagg agtcattttt
    10441 accgacttgt tcactgcaat gatcgaatat ccagaactag tccaagaata ttatatgaaa
    10501 aaagcagtaa acatgaacga agaccaattg actgcactcc atgtagcttt tatgaacagc
    10561 ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg aaccactcga gtccttattc
    10621 attcaagatg gcgcatccga tgagcacttc ttcaagcatg tgttgatcgt ggcagatgaa
    10681 catagtgaat tttcttatct ggaacgtttc cagacaacca aagaacaagt agcaaaatca
    10741 tcaggtaata tcatcgtgga agtcatcgct aaagctggtt caaaaatcaa atattcagcc
    10801 gtagatcaat taggagaaaa tattacttct tatatgaatc gccgtgggca cattttacga
    10861 gatgcttctg tcgactgggc aattggtgtc atgaatgacg gtcatgtgat cgctgacttt
    10921 gactcagatt tagcaggaga aggtgcacac gcagaagtca aaatcgtggc aatcagctcg
    10981 ggcagacaaa tccaaggaat cgatacacga gtaaccaata aagcccctca tacgatcggg
    11041 catattttgc aacacggagt gatccgagaa aaaggaacat tgacctttaa tggtatcggc
    11101 cacatcttga aaggggcaaa aggtgcagat gcccaacaag aaagtcgtgt cttgatgctt
    11161 tctgataaag cacgtggtga tgcgaatcct attcttttga tcgatgaaaa cgaagtaact
    11221 gctggacatg ctgccagtgt aggacgagtt gatccagaag aaatgtacta tctgatgagt
    11281 agaggattgc ataaagaaga agcagagcgc ttagtgattc gaggattcct cggttctgtc
    11341 ttaacagcaa tccctgtaga acaagttcgg aaagaattag tcgaagtaat cgaagggaag
    11401 ttgaatggat gatcgatgct aaaaagctac gcaatgattt tcctatctta tttcaagacg
    11461 tcaatgatga acctttagtt tatctggata atgcagcgac gacccaaaag ccaaaacaag
    11521 tactccaggc attagcgtac tattacgaac acgataacgc caatgttcat cgcggtgtcc
    11581 atacgttagc agaacgcgcg acccatgatt atgaacaagc aagagaaaaa gtacgggcgt
    11641 tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg tggtacgacg acaagcttga
    11701 attgggtagc cagaaccttc ggcgaccgct atatcaaagc aggagacgag atagtgctct
    11761 cttacatgga acatcattct aatgttatcc cgtggcagca gttggcaaaa cgaacaggtg
    11821 ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct ggatatggag gatgcagcga
    11881 aaaagattac ggacaaaacc aaaatcgtct ccattgctca tgtatccaat gtcttaggtg
    11941 tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga aaacggcggg gtcatcgtag
    12001 tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt ccaagcacta gactgtgatt
    12061 tctttgcttt cagcggacac aaaatgtgcg gaccaacagg tattggtgtt ttatatggta
    12121 aacgaaaatg gttagaagaa atggaacctg ttgaatttgg tggcgaaatg atcgattttg
    12181 tcgaattata cgacagtaca tggaaagaac taccttggaa attcgaagca gggacaccaa
    12241 atatcgccgg cgctatcgca ttaggccatg caatcgatta tttgcaaaaa ctcggtatgg
    12301 acaatatcca tcgatatgaa gaagaactag ccgcttatgt gttgccaaaa ttacaagcaa
    12361 ttgatggatt gactgtttat ggtccacaag acccgaattt tcggacagga gtcgttgcat
    12421 tcaatttgga tgggttacat ccccatgatg tcgcgacagc tttagacatg gaaggggtag
    12481 ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa gtaccttgaa gtatcagcga
    12541 ctgctcgcgc gagcttttat ttctacaaca caacgcaaga cgccgaccgt ttagttgaag
    12601 caatcatggc aacaaaggag tttttccaac atgggactat ctaaattaga caatttatac
    12661 cgtcaagtga tcttggatca ttcttcccat ccgcatcatc gtggaagctt gactgactcg
    12721 acaagcaaga tcgaattgaa caaccccaca tgtggagatg tgatcgaatt gcaagtcgat
    12781 gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca gtggctgctc catcagtacg
    12841 gcaagtgcct cgatgatgac agacgccgtc atcggaaaat caatagaaga agcagaacat
    12901 ttaactgtag actttctaca gcttgtccaa ggaaaagaag tcaaagatcc cgaccaatta
    12961 ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg ctcggatcaa atgtgcgaca
    13021 cttgcctgga aagcactagg aaaagcaatg gaaaacgatg gtaccgcagc tttccatcat
    13081 taccacaagg aagaggagtg agaacatggg cgtaccagaa ttagaagaat ataaattcgg
    13141 gtttcacgat gatgtcgaac cggttttcag tacaggagaa ggcttgacag aagaagtgat
    13201 ccgtgagatg tcaagaatca aaggcgaacc agaatggatg cttgaattcc gtctgaaatc
    13261 tctagaaaca tttaacaaaa tgccaatgca aacatggggc cccgaccttt cagacatcga
    13321 tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa cccgctcgtg actgggaaga
    13381 cgtaccagaa aaaatcaaag atacatttga aaagattggt ataccagaag ctgaacgagc
    13441 ttatctagcc ggtgcttccg cacaatatga atcagaagtc gtgtaccata atatgaaaga
    13501 tgaattcgaa aagttaggaa tcgtctttac tgacactgac tctgctttga aagaataccc
    13561 tgaattattc aaagagtact tttcaaaact tgtaccgcca accgacaaca aattagcagc
    13621 gctgaattca gccgtatggt caggtgggac ctttatttac gtgccaaaag gcgtcaaagt
    13681 cgatgttcca ttacaaacgt acttccggat caatgcggaa aacacggggc aattcgaacg
    13741 tacgctgatc attgttgacg aaggagcaag catccactat gtcgaaggtt gtacggcacc
    13801 tacttattca agtaacagcc tgcatgccgc catcgtagaa atctttacaa gaaaagatgc
    13861 gtattgccgt tacacaacga tccaaaactg gtcagacaat gtgtacaact tagtaaccaa
    13921 acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc gacggaaacc taggtgcaaa
    13981 aacaacgatg aaatacccaa gtgtttactt agatggaaaa ggtgcacgag gcaccatgct
    14041 ctccatcgcc tttgcaggag caaaccaaat ccaagatacg ggtgcaaaaa tgatccacaa
    14101 tgcaccaaat acatctagct ctatcgtctc taaatcaatt tcaaaagacg gcggagaagt
    14161 aaactatcga ggacaagtaa cctttgccaa aaacagtgcg ggttcgatct ctcatatcga
    14221 atgtgacacg atcatcatgg acgaatggtc gaaatccgat acgatcccat tcaatgagat
    14281 ccacaacagc caagtctctt tagaacacga agccaaagtc tcaaaaatct cagaagaaca
    14341 actctactat ctgatgagcc gaggcctgac cgaatctgaa gcaactgaaa tgatcgtcat
    14401 gggctttgtt gaaccattca ccaaagaact tccaatggaa tatgcagtcg aactcaatcg
    14461 attgatcagt tatgagatgg aaggcagtgt tgggtaaacc tgttatagga gctagaaccc
    14521 ttgatatata agggttctag cttttttgtt ttgcctgtat actagaaatg accagatttt
    14581 gaccagctct tttactgatg agccatcata tcagcaaagt cttgacctgt tttcttaggt
    14641 gcattatttg taatatgtgc atagatgtct aatgtcactt tactattttc atgccctaat
    14701 cgttgttgaa ctaccttgac agattctcct gcttctaata ataaactagc atgtgtatgt
    14761 ctaaacccat gaggtgtaat acgaggcaag ttatattttt tcaaaatata tcttaaccaa
    14821 tcattaacag tgtttggaat gtatagagtg tttttaactg ttgtaaaaac atgttgctct
    14881 ttagagctag tattgtatcc aagttttaaa tactcttctt tttgctgtaa tttccagttg
    14941 ttgaggatag aaagagtctc agtatctaaa gagataactc gtctagaagc tcttgttttc
    15001 ggagattgaa ttataacttt accaaattca tctacagcaa gagttttatt gacatgtagt
    15061 tctttattaa acgtgtctat gtctttccac tgtaaagcga gtacttcact ctttctcata
    15121 cctgtgtatg ccaataatct gaagaaggcg tattgtttca tgttaccaaa atctttaaaa
    15181 gcatccagta aattattaag ctcctctttt gagtaaaatt tacgattttt ctcatactct
    15241 ttttttcgtg gaagaagtgt tttttccata ggattattgt caatatattg cattgcaacc
    15301 ccgtagcgta aaatttgtgc agtctgtttt ctcatataat cgtaactttc atatttactg
    15361 tgccattcat ctaccacttt ttgacaatat gggacagaga tattcgatag tttaagctta
    15421 ccaaatgctg gaagtatttg attatttgcg tatcttactg agattgcgat tgttgaagct
    15481 ttaacactgg tacgctgatg agttatccat tcattataca ggtcttcaaa tgtcatatca
    15541 ggtgctttct ttttaacacc gtataatatt tgatgtctgt actcgtcata atctttttga
    15601 gcttcttgtt tgctactaaa acctcttctc gtagtattta ttttttcacc agttcgtgga
    15661 tcggtggcaa tgtgtccttt atacatccac gccacttgac cgtttttctt tttgtacttt
    15721 gatatttttg ccattaaatc atctcctctt agtttacaag gagtacttac cactagatgg
    15781 tatcacctcc cttcattttt ttcttagtgt attttttccc aattgatgaa atcgtttgtt
    15841 ctagcttgtt tatattatca gtaatttctc tcacagcttc ttcgggataa gacatgtctt
    15901 tcaattgaat aggtagccat tcatctacca ttctttctag tagttcttct atactgctag
    15961 tgaggaaaag aatgttattg tcaaattctt ctacaatagc attgatgaat aattcttcta
    16021 tttttaagat attgaaaaga ctaatttttt catttttaac tttattacaa gtcttgttag
    16081 caataaattc ttgcatctct ggttctaatt tggaatatct atccagccat gtatctaaat
    16141 tagagtgtac tggtgggaac tgttgacgaa gatgctggaa caggttttta tctggaaatg
    16201 ttataccatc tagtacaatg ctatctctat aaagaataat tcccaaaata tattcttcta
    16261 cattgccaca aactaactcc tctatactca tatttcccaa atcagcaatt cgggataatc
    16321 gctctggtat tggtaaatag gttcccttct cccattcgta gatagttttt ttgtcaatta
    16381 attgaccgtt gttaatcata agaccaaatg ctggttgtgt taatttttta ctctttctta
    16441 tagcttttat ttttgttccg agtatctttt ttcgttctaa ataatttatt ggtggacgtt
    16501 ttatcatatt gaccacacct cctaaaatta gtatacctca aaaaaggggt gttgcaaata
    16561 aattttacaa agctgttgac accccttttt tatcgaggta ttatgtgtat gtcgatgaac
    16621 tggtaatcat tttttgtttg attaatcggt taataacaaa atacttttat tttgagcaaa
    16681 aaatacgaag ataattctta gaaaatttgt tcaagcaatc acgaaaggag ggggaaatat
    16741 gtctctacaa atcattgata gcattgtttc tatacaagtt gaacaacaag tgaaagagat
    16801 aattacagtg ctaaagccta tgctagaaga cattatttct ggagtcactc tagagtatat
    16861 ggactatcag caggcaagtg aatatctagg tgtatcagta ggaacgatta gaaaatatgt
    16921 ttctcaatac gggttacctg ttataaaaat tgatacggtc attagattta aaaaatctga
    16981 tattgatagt ttcatggaac ggtataaagg ttgagtaaac aaataagact aaaacatata
    17041 gtaataaaga gtttgttttt attatctgta tgttttagtc tttaacagaa ttttataagg
    17101 aggaaacaca aatggaaaca tcaatgatta atattccaat ttatgtagat caagaaaaat
    17161 tattagagaa gccagttaca aaaaaagaaa aaactgatgt aagagatagg tgtttagata
    17221 actatagaga agttacaaga gatgagtttg tagagatact aaattcatca caatcattta
    17281 ttccaagcaa gacaaagaat aaaggcaatg acaaggagag ttttgtggaa accagagtaa
    17341 ttattctgga tgtcgataac actgttaaag atgagaaggg gaaggtagta gatttgagta
    17401 aagatgatag tagatactta agcgtagaaa aggttttatc tatcaattta gttcataatt
    17461 ctgcgtttgc gatacaaaaa agtattcggt actctgaaaa tctagaaaaa tacaagattg
    17521 tctttctatt aaaagaagca attactgact atggtaaaat ggttgctgtt tacgaatact
    17581 tgaaaaagaa catccccggg tgtgatgaca atgtaaatgc ttcgaataga atgttttttg
    17641 gaggatataa gtcagatgca gtgataataa ttaatgaaga caacatgtta gatattactg
    17701 agcttccgat tgattttacc atgacatgtt ctaaaaattg tcaaagtgat tcagggagag
    17761 agtatgaact agtcaatgaa accgattttg ttaagttgat aaaaaatgac gacaaagaag
    17821 aaatgaaaca gtggtttaaa gactgcctgt ttgattcctc cgacatgtct tttaatgaga
    17881 tttatgaaag attgttaacc attgatatga ataaattact taagtcaagt aaaaacttaa
    17941 gatgtctgtt tcacaacgat cataatccct ctgctagtat ttttaccagt aaaactgggc
    18001 attccatttt ttattgtcat tccagtaatt gtgatatcag tgaaaatttt attggagttg
    18061 tgatgctaat tttaaagaaa gacagtcggg tagaaacatt taattggcta ttagagaacc
    18121 tagacttgta tcctacacat tttcgaaaat tgaaagagga atcaaaagta ctttttgaca
    18181 ctcttgaatc aatgaaagac aaacttttca ggacaataag gaattatcta gaggaaatgc
    18241 gacgtgtcta tgatgtcttg ctttctgatg ttaatttttt tgatgatagt aaggaaagtg
    18301 tgacttgtat tttgtcgggg gaacaattag ctaaaaaaat gagttcttac tatggtaaaa
    18361 cttatgacat agataaatgt aataagttac tatcacttat gacttttata ggattgataa
    18421 aaaaattaga tgatgaagat gttcctttga agatgctcga atacctaaat aggttaaagg
    18481 aagagaaagc gttaggaaac tttaatcata agagaagcaa tgtctatcga ttagacgttt
    18541 ttaatgcaac ttctgtgatt caaaaattga atgaagatat ccttccactt ctggaaaaaa
    18601 ttcattttac atatcaatat ttttcttatg aatgggttaa gatatgtttt aacgaaaaag
    18661 aggctaaaag agtatttcct caaaattccc gatcagaact cagcaaggaa aaacaattga
    18721 ttttagatga agcacatgaa gttttacagg gcttatcgtg gcaagggaca tatctaatga
    18781 cagagaaaga acttattaac actgtttgtg aacgcctacg taatgttggg gataacagcg
    18841 tgaaaaatgc tttaataaaa aacagaggct actttgttaa acaaggattc actcttgaac
    18901 gagcttctaa ggatattaag aaactataca atgtgaaagc aagtagtagt tttttaatat
    18961 atttagcgaa aggggcaggg atagatgaaa caataaaaga attgatatta tcaggagctg
    19021 ggatagttcc tctgtatcaa tttacaagtg taacaagaga tgatggacgg agagtaagga
    19081 agagaaaaga cccaattgta ttaaactaga caggttatcc atagctctta atcttatttc
    19141 atctcaatac acccaccgtt aaagttcggt gggttattta tttttcaatc tttcaatagc
    19201 tgattgcgtt cattttcttg cctttttaat agcttcatat agtcaattgt tcgaactgca
    19261 tcttcagtgc taagagaacg atagagagcc aaaaactctt cctcataatc tacgacaaca
    19321 ttgtctagaa aatggcttac aggggttcct agggctctag aaagagctgt taagctatcg
    19381 aggttaggct ctgttacaga attttcccat cttgctactg cctgttgtgt agagaagatt
    19441 ttttcagcta attctttttg tgtccacccg agagcttttc gcttttctct aatgaccttt
    19501 gcatattgtt tctgtttcat taatacaccc ccatctcagt atagtacaac aagttgttgt
    19561 atttgtaatg gtatttgaag ttttttatat tttatgagtt gaaatacatc atattgttgt
    19621 atataataaa acaggagggg agaatcatga aaaaagtaag taatctcagc cgatacagaa
    19681 aagaaaaagg actttctcaa acggagttag caaaaaaaat gaatgttaca cagcaatgtg
    19741 taagttcgtg gcaaacaggg agaacgattc ctaaacccta tcaaatgaag atgctttctg
    19801 agatattaag tgtaccaatg aatgagctgt tttctgatgt atttaataaa gtaaacagtt
    19861 aactaataag caattgaatg attttaggag gagaagacat gtattgtaga aaatgtggca
    19921 aagaaataac agatgaaagt aagtattgcc agttttgtgg agcactacaa gaaacagatg
    19981 agacaaccgg aacagacaca gtaaagaaaa gtactgagca gacaattaat gaaatgaagg
    20041 aacaaatcgt acatggacaa gagacaggga agctgtattt gattattggt tggatttcta
    20101 tggtagtatc gttagcattt attcctgttg tatttggtgc tgttgctgtt attatgggct
    20161 acttatatcg agaaaaagat gagaaaatgg gtacgatttt gatgatagca ggggtagctg
    20221 gagcaatctt tggtgtactt ttagggatgt caatgtactg ataagaggga taagagcaa
//
LOCUS       DACDSR010000047        19821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDSR010000047 DACDSR010000000
VERSION     DACDSR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.047"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWK15_001688"
     CDS             6..305
                     /locus_tag="IWK15_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9547275.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWK15_001689"
     CDS             378..1739
                     /locus_tag="IWK15_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9547276.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWK15_001690"
     CDS             1761..2021
                     /locus_tag="IWK15_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547277.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWK15_001691"
     CDS             2035..2787
                     /locus_tag="IWK15_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9547278.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWK15_001692"
     CDS             complement(2881..3486)
                     /locus_tag="IWK15_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9547279.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWK15_001693"
     CDS             complement(3539..4513)
                     /locus_tag="IWK15_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9547280.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWK15_001694"
     CDS             complement(4891..5670)
                     /locus_tag="IWK15_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9547281.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK15_001695"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK15_001695"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9547282.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWK15_001696"
     CDS             7161..8558
                     /locus_tag="IWK15_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9547283.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWK15_001697"
     CDS             8639..9079
                     /locus_tag="IWK15_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9547284.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWK15_001698"
     CDS             9227..9553
                     /locus_tag="IWK15_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9547285.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWK15_001699"
     CDS             9554..10303
                     /locus_tag="IWK15_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547286.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWK15_001700"
     CDS             complement(10383..10838)
                     /locus_tag="IWK15_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9547287.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWK15_001701"
     CDS             complement(11265..11540)
                     /locus_tag="IWK15_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547288.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWK15_001702"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWK15_001702"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9547289.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK15_001703"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK15_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9547290.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IWK15_001704"
     CDS             14770..17436
                     /locus_tag="IWK15_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9547291.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IWK15_001705"
     CDS             17800..18552
                     /locus_tag="IWK15_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9547292.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IWK15_001706"
     CDS             complement(18574..19728)
                     /locus_tag="IWK15_001706"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9547293.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DACDSR010000048        19475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDSR010000048 DACDSR010000000
VERSION     DACDSR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.048"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWK15_001707"
     CDS             complement(21..995)
                     /locus_tag="IWK15_001707"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9547294.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWK15_001708"
     CDS             complement(1075..1950)
                     /locus_tag="IWK15_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9547295.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWK15_001709"
     CDS             2080..3987
                     /locus_tag="IWK15_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9547296.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWK15_001710"
     CDS             complement(4528..7806)
                     /locus_tag="IWK15_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9547297.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWK15_001711"
     CDS             complement(8077..8622)
                     /locus_tag="IWK15_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9547298.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWK15_001712"
     CDS             complement(8634..9326)
                     /locus_tag="IWK15_001712"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9547299.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWK15_001713"
     CDS             complement(9345..9629)
                     /locus_tag="IWK15_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547300.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWK15_001714"
     CDS             complement(9642..10205)
                     /locus_tag="IWK15_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9547301.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWK15_001715"
     CDS             10433..11095
                     /locus_tag="IWK15_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9547302.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWK15_001716"
     CDS             11219..12415
                     /locus_tag="IWK15_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9547303.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWK15_001717"
     CDS             complement(12516..13892)
                     /locus_tag="IWK15_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9547304.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWK15_001718"
     CDS             complement(13929..14795)
                     /locus_tag="IWK15_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9547305.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="IWK15_001719"
     CDS             complement(14884..15909)
                     /locus_tag="IWK15_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9547306.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWK15_001720"
     CDS             complement(15927..16601)
                     /locus_tag="IWK15_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547307.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWK15_001721"
     CDS             complement(16785..17678)
                     /locus_tag="IWK15_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9547308.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWK15_001722"
     CDS             complement(17752..19449)
                     /locus_tag="IWK15_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9547309.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DACDSR010000049        19130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDSR010000049 DACDSR010000000
VERSION     DACDSR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.049"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWK15_001723"
     CDS             complement(<1..703)
                     /locus_tag="IWK15_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547310.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWK15_001724"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWK15_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWK15_001725"
     CDS             1758..2837
                     /locus_tag="IWK15_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9547311.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWK15_001726"
     CDS             2869..3462
                     /locus_tag="IWK15_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547312.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWK15_001727"
     CDS             complement(3572..3799)
                     /locus_tag="IWK15_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9547313.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWK15_001728"
     CDS             complement(3902..6490)
                     /locus_tag="IWK15_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9547314.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWK15_001729"
     CDS             complement(6510..7169)
                     /locus_tag="IWK15_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9547315.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWK15_001730"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWK15_001730"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9547316.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWK15_001731"
     CDS             complement(7942..8808)
                     /locus_tag="IWK15_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9547317.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWK15_001732"
     CDS             8917..9558
                     /locus_tag="IWK15_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9547318.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWK15_001733"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWK15_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9547319.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWK15_001734"
     CDS             complement(10999..11898)
                     /locus_tag="IWK15_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9547320.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWK15_001735"
     CDS             complement(11900..12697)
                     /locus_tag="IWK15_001735"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9547321.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWK15_001736"
     CDS             complement(12675..13379)
                     /locus_tag="IWK15_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9547322.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWK15_001737"
     CDS             13510..14085
                     /locus_tag="IWK15_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9547323.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWK15_001738"
     CDS             14203..14880
                     /locus_tag="IWK15_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9547324.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWK15_001739"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWK15_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9547325.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWK15_001740"
     CDS             complement(16943..17941)
                     /locus_tag="IWK15_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9547326.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWK15_001741"
     CDS             complement(18167..18820)
                     /locus_tag="IWK15_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9547327.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DACDSR010000050        18810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDSR010000050 DACDSR010000000
VERSION     DACDSR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.050"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWK15_001742"
     CDS             complement(55..789)
                     /locus_tag="IWK15_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9547328.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWK15_001743"
     CDS             1116..1526
                     /locus_tag="IWK15_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9547329.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWK15_001744"
     CDS             1519..2220
                     /locus_tag="IWK15_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9547330.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWK15_001745"
     CDS             2220..3806
                     /locus_tag="IWK15_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9547331.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWK15_001746"
     CDS             3825..4046
                     /locus_tag="IWK15_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9547332.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWK15_001747"
     CDS             4043..4804
                     /locus_tag="IWK15_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9547333.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWK15_001748"
     CDS             5184..5693
                     /locus_tag="IWK15_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9547334.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWK15_001749"
     CDS             complement(5768..6307)
                     /locus_tag="IWK15_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9547335.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWK15_001750"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWK15_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9547336.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWK15_001751"
     CDS             7374..8171
                     /locus_tag="IWK15_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9547337.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWK15_001752"
     CDS             8199..8834
                     /locus_tag="IWK15_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9547338.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWK15_001753"
     CDS             8854..9627
                     /locus_tag="IWK15_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9547339.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWK15_001754"
     CDS             10035..10832
                     /locus_tag="IWK15_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9547340.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWK15_001755"
     CDS             10810..11223
                     /locus_tag="IWK15_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547341.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWK15_001756"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWK15_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9547342.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWK15_001757"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWK15_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9547343.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWK15_001758"
     CDS             16000..16560
                     /locus_tag="IWK15_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9547344.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWK15_001759"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWK15_001759"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9547345.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT         6070 a         3438 c         4015 g         5287 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct
//
LOCUS       DACDSR010000051        17993 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDSR010000051 DACDSR010000000
VERSION     DACDSR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.051"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(33..611)
                     /locus_tag="IWK15_001760"
     CDS             complement(33..611)
                     /locus_tag="IWK15_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9547346.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(631..1299)
                     /locus_tag="IWK15_001761"
     CDS             complement(631..1299)
                     /locus_tag="IWK15_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9547347.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1314..2384)
                     /locus_tag="IWK15_001762"
     CDS             complement(1314..2384)
                     /locus_tag="IWK15_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547348.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2555..3919)
                     /locus_tag="IWK15_001763"
     CDS             complement(2555..3919)
                     /locus_tag="IWK15_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9547349.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3916..5475)
                     /locus_tag="IWK15_001764"
     CDS             complement(3916..5475)
                     /locus_tag="IWK15_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547350.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5531..8011)
                     /locus_tag="IWK15_001765"
     CDS             complement(5531..8011)
                     /locus_tag="IWK15_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547351.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(7995..8330)
                     /locus_tag="IWK15_001766"
     CDS             complement(7995..8330)
                     /locus_tag="IWK15_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547352.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8344..9378)
                     /locus_tag="IWK15_001767"
     CDS             complement(8344..9378)
                     /locus_tag="IWK15_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9547353.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9468..10148)
                     /locus_tag="IWK15_001768"
     CDS             complement(9468..10148)
                     /locus_tag="IWK15_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9547354.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10432..11535)
                     /locus_tag="IWK15_001769"
     CDS             complement(10432..11535)
                     /locus_tag="IWK15_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9547355.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12164..12634)
                     /locus_tag="IWK15_001770"
     CDS             complement(12164..12634)
                     /locus_tag="IWK15_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9547356.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            12964..14604
                     /locus_tag="IWK15_001771"
     CDS             12964..14604
                     /locus_tag="IWK15_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9547357.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14758..16080
                     /locus_tag="IWK15_001772"
     CDS             14758..16080
                     /locus_tag="IWK15_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9547358.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16148..16582)
                     /locus_tag="IWK15_001773"
     CDS             complement(16148..16582)
                     /locus_tag="IWK15_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547359.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16647..17484)
                     /locus_tag="IWK15_001774"
                     /pseudo
     CDS             complement(16647..17484)
                     /locus_tag="IWK15_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         5243 a         3671 c         3188 g         5891 t
ORIGIN      
        1 cacctaacta tttttctccc tatataacag ctttacgctt ctcctaatac accattcaaa
       61 tataaatcga tcaattgcgc cacatttagc ggttcttcca caggtctttt cgaccgagtg
      121 atgtaaggtg ctaacactaa gaaaaaatgc cttgctgcga tttcttttga aatcccattt
      181 cttagcacag attggtttgc tgaaaagatt tcttcaaaag gttcacgata atttttcttc
      241 catagaaaga aaagatgatc tcttgtttcc tttgaaagat tgctttcaag atcgtgaagc
      301 atcagaaaca aatcgaccag atgtgtctca atcaaaaact gcgccatttt tgtcaaggtt
      361 acttgaaatc ccgtttcgct tgtttgaacg atttctctca aagcttttcc aacatctttt
      421 aagtggtttt ctaaaactgc cgtatatagt ttttctttat caccgaaata atgatagaga
      481 ttgggttggg tgatcccaac tttttgtgcg atctctcttg ttgaagtgcc gagatatccc
      541 ttggacataa acaagtcagt cgcttctttc aaaatttttt gctgtgtatc tttgttgtat
      601 cgatggacca tttttccacc tgctttttgg ttattctttt tctttcaata caccatcttc
      661 catcataaat attctatcgc atttgtcaat cagccgtgta tcgtgagtaa ccatgatcgt
      721 tgctttattt ttgtcttttg attcttttgc caagatatcg actacttctc gtgccttttc
      781 tgaatccaaa ctagcagttg gttcatctgc tagaataatg gacggatcat tgtacaatgc
      841 acgagcgatt gcgacccttt gccgttctcc accagatagt tcttctggat acttgttgat
      901 caacttatcc acgccaagct gctcaaataa ttcttttgct tctttttgac gatgttcatg
      961 tttgatctta tctactaata ataactgctt ttttactgtc agaaaaggaa ccagattgga
     1021 agcttgcaaa ataaagccaa tttctttaaa acgtagttta acgcgttcac tttctttttg
     1081 attactgaat tcttgatcat tgataaatac agagccactg ctaggcgtct gcaatccgcc
     1141 tgccaaagtc aaaaaagtac ttttacctga accagaagga ccgatgattg caacaaattc
     1201 ccctttatcg atactcatgg ttgtttcttt caatgcttta actacttggt cgccatcttg
     1261 gaaacttttt tctacttctt taaattctat tgctttcatg ctcttcctcc ttcttatcca
     1321 attgccttca acggatcgat ttttacgatt gtccgaacag aaaatagtgc gcctaacaca
     1381 gctactacca gcatcaacaa gctaatgacg ccaaaaaaca gccaattgct ttggaatgga
     1441 accgcatcag gtagaacaag ggatgttccc actgttccta atagaccaag aagaatacca
     1501 acaaatgata aaatgaatgt ttgtgccact acagaacgcg caataaatcc tcctgtgatc
     1561 ccttgtgctt tcattacacc aaagatatta atcttttgca tcgtcagtac ataaatgaaa
     1621 atcccgatca caatagccgc aataacgatc aaaaatccga tcataaatcc aaaagtcaat
     1681 acttgcgcat tatatccagg taattcattg atgaatttag aaattttgat ctgttctaaa
     1741 tcttcaggta aattactgat tttacctctg acaactattg cgttaatccg tgcattttca
     1801 cttgtatctt ctttttcgaa gcggatctct tggtaagcat tgatggttgt atagagaact
     1861 ggtgagacat tgaatttggc atgatccgta aagccagtta gtttaaacgt cttgtcacta
     1921 ccagataact taactgtatc tcctacggct agaccatatt cttctttcaa actaatatcg
     1981 cctactgcct catcatcgtt atcaaaagct tttccttcaa caagattggg catgatgaat
     2041 tgatttttat cgataccgaa gaagctaaca ttgatttttc cttcttctgt actgtcttta
     2101 ctagtcacga ctccaggtgt ctgggcaaga tacgccactt tcccaccttc gacttcttct
     2161 gctatcttct tagtaatcat ggacatccct agatttgaat ttgcatcttt cgaaagcaca
     2221 atcgaatctg cctcccactt gtcgactgct gttcgattat cttgagctaa gccataagct
     2281 aacccagtca aaaaaaatac caggtaggcg atcaaaaaca tcacccctgc aactaacgca
     2341 taacgtaact ttgaatgcat aatttcattt aatgctaaaa acatttaact cctcctcctg
     2401 aaataatcat tttatacaac aattttatca cttgataaat ttttggcgaa tattatgctc
     2461 aaaaaattct tccgctattt cattatatga attttttatt ctcgattgat taatatttat
     2521 tatatcccca cacaataaaa gaccgtgata tttctcacag tcttttatct actctattgc
     2581 tatttgctct cttagctagt actttaaaag ctaactcatt gaggtagtga aaacgagagg
     2641 tgtgttcaga cagaaatcca aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt
     2701 tcacgatatc cgcaagcagg ttcaaatctg ataataagaa ttcttggctt atcagctgtc
     2761 cgccaattat ttctaatgta tccggatggt agatcagctt tgcatttact gttttgtttt
     2821 cttcatcgta ttgtggaaat agatcaaatt ctcctgaaca cgaaccggta ttttgttcca
     2881 agaatggcgc ttcatcttct gtcagcccta cagttcctaa aaagtagtct ataatgactg
     2941 ctgctgttat cttttgtctg aggtcgtaag cggttggctg accactcaaa gttagcgcta
     3001 ctgctcttgc catatgtaca gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt
     3061 tgtcaaaact gactgggacg atatctccaa tcgcatagat gtccggatca cttgtctgca
     3121 gatattcatt gactatcaca gtctcatcct catacaaagc cactttttct tttaacaatg
     3181 tcgtattagg acgagtttgg gcagaaaaaa tgaccatatc tgcctttatt gcttgagata
     3241 gaagttgtac ggatactact ttctcttctg attttttcca atcgatcaag aattcatttg
     3301 gataaaaatt gatttgctta gtttgcagct gattttgaaa actgttgatc atatccttat
     3361 cgaaatatct tgataaaaca ttcggcatat aatcaactag atgaataatg tatcctttat
     3421 ttctcaaagc atctgctaat tccaatccaa tataaccagc accgaagatc actacttctt
     3481 ttgcttcttt taaagcggca agtacttgct tagattgcac tagattttta taggtgtaga
     3541 cattttcagc ttcaatttgt ttatacaagg tgacaggaga ggaacctgtt gctacgatta
     3601 atttatcata ggttatggtc tcgttttttc cttctttgga tgcgaggatg agctgatgtt
     3661 tgtctggatc aagttcagtt actctccatt cggtgatcag ttttgcccca ctttcttgaa
     3721 gagtccgcat gttatgggca acttcagcta aactacgtat ctttcctttt aaataaaggt
     3781 tgattccatt tgaaacatag cctagtccat ctttatgatc ttcatcgatc agtaaaactt
     3841 ctgctttcgg ttgaagtttt cttagattta gggcagctgt gatgcctgca tgggaagcac
     3901 caatgatcac gattttcatg ttttattcat ccttaaattc aatttttcta aataacattc
     3961 ttttaaaaat tggttcaaat aggggaaatc ataattatat tcgttgctag tgaatacaaa
     4021 aactttagcg gacttctgtg acgggtactc tttcaattgg ctagtcaaaa tgatatcata
     4081 ctgcttttct ttctcgtatt cgactaaatc atatttgatc gtattcagca attgttcctg
     4141 gatgatttct ttcacgatat ccgccattag cctttcataa ggaagatcat aagcaatttg
     4201 cagacgtaat tctaactgct tgatcagcgt ccgcaaaata gatgcatagc gagcaaataa
     4261 ttcaaagcgg gcattttcct cgtctacttc agtaaaatca acaatagcaa atgtttgtct
     4321 gaccaatctt tcacataaaa ccaggaattg tttatcttct acttcagtta accgctgttt
     4381 taatccttct tggccaaaaa tattcatcca tcctctaaaa taggcaaaat gagcgtgatt
     4441 ttgcatcact gtcaacgcaa tcttttgcga aatctcttct ggaaaaacat aaaaagagaa
     4501 atattcttct atcgattgtt taaacgttcg attcaattga gctactacgt cgtctttgta
     4561 tttttcgctt atcaaaaact cctggacatg cgagttatgt gtatctaatg taaagttact
     4621 gatcatgaaa agataaaaaa ccaacgtttc attttgattc caaggaaagg catattgaaa
     4681 gaagtaacgg gtcaaaaatg tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc
     4741 aacaggaaga ttgctataat ctggtttgga ttttttcgta cagcgcattc gcttcttcat
     4801 aatggaaagc catatttcaa atttcaatcg tccttcttct gtaatagtaa aactaaactc
     4861 tttttgcagc agatctaaaa aagtgtttcc tgataaatca ctgtattctt ttttgttttc
     4921 aagataagga cgtccaaacc aaaataattg gaaataaaaa taacaaattt gactttctgg
     4981 accggtcagc cgcccgcttt ttattttcag atgaaactca tttaaagaat catttaattc
     5041 cttcactctg cgagacaaag tagccgaact gatattaagt tccatgacaa gtttctgccg
     5101 atcaaatgag ccattttcca acagataaag aagtatctga tattttgtag aatagcgcaa
     5161 gtagcaatga taataaacat cgactgctgc tatatttcca tgtctgatta ataaaatatt
     5221 cccttcttgg ataacaattt ccaattgttt gatattttcc aagttagctt gtaaataaaa
     5281 cagatcttct ttcaaggtag cccgatcaac tgataagcgg gtaagtacgt tattaacgga
     5341 aatctctcct ttttcctcta ataaaagttg gaaaagtttg atttgccgtt gttcattttt
     5401 ttcaagcaaa ctgaaaagtt ccaagtccac tcctccaagc atttctgatt ttaacagcag
     5461 aagtttcctt ctcatccttg aaaaagagga tctgtgacag ttatcacaga tccatacaaa
     5521 ttctaatcac ttagctggtt ggcccagctg tcagttccca tgtgaccatg ccttgatacg
     5581 ttgcgccagg ttgagcattc gtactgctat ttacctctaa gagtaaacca tcggtatcgg
     5641 gaaagtcaac gagaacctct cctttagctg ttccatcact ctcataaatc aacgtgtttt
     5701 gaccttgctg aatcaattgt tttgatccag aatgattgaa gtataatttt gttccgctta
     5761 attcttgacc ttgatcattc ttaaataaag aagtcaatgt ccctgtcagt tgccaaggag
     5821 acgtgcttgg aagccgagta tcccgcacat caaaagaaaa atcgcctttt gcttcataat
     5881 aacttgtctt caacggtttg gggagatcgt taccaaatgt aaaatcatct ggtacattaa
     5941 ctaatgttaa ttcttgtttg gggacaagaa ttgttttagc agatgtttgg acaactgggt
     6001 taggaagctt agcaccatct gctaatgtaa tattgctgga aagtacagct gcctttgtca
     6061 gtatgctgac ggattgtaca gaagtatcta cagtatttaa ttgatagttg taagtaagat
     6121 gaatggtatc tcctattttg ctcaatagat ctgctgggac agaaactcct tgttgaatgc
     6181 cggtatctgc gatagttgta ccatttaatt ttaaggaacc attgatataa gtagtatttt
     6241 caggaagtga gatcgtgatt tttttgtcgg ttatcgcatc cccagatttt gttaacgtga
     6301 atgtcacatt ttcttgaatc gtatctcctg atagagcaga caggggatct cctgtctcag
     6361 gttgtctgct aaggtttttt agctgactat ctgcactcac agatggaaaa ttagccactg
     6421 ttatttgagg aagagtggtg attccagctt gttcagactg ccatttgtac gcaaaagacg
     6481 gatacatgtt agtaagtaca tcgtccacca agcgataatc aaaataatat tgccctgaag
     6541 ccaatgcagg tagtgtagaa gtagctgtat ttacaccccc gttatttgat tggatcgttt
     6601 ggaaaagttg atctgtccat cctgtcgcta atttttgggt tcctttcgaa taaagcctgt
     6661 aatggatcga accctttttt agttcagttg ctgaccaatt ggccgtgact tggtacgatg
     6721 cagaagaaag gagctttccc cactgactat cggctgttaa gtttgttggt aaaaccgttc
     6781 cgtctagctt gtactgctcc aataccttta aagcaacagg agaaagcaat ccgccttgcg
     6841 catctttccc actgtacacg atattggctg tctgattcaa tccacttgtg cttgctggag
     6901 tatgagtcag tattgctcct tgtgtaacgt tttttcctgt aaaatagttg actacgcgat
     6961 caaagcgact aaatggcaac tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc
     7021 gactattggt agagcctgga aggttctggt tatttccatc cacgtattct gtggtatcag
     7081 caaatgtatt atattttcct gttgaatcac ttgttgagat gtaactattc cacgtattta
     7141 tctctgcact tgcagaaaat gcatatgctc ctggattcaa gtttccgcca gttaatttag
     7201 gataaaatct acctgcagct ggagctaaaa tcgaattgaa catacgatct tggataatat
     7261 tattcgaatc ataaaaatca acagaaaaaa cggccttttt agacatagaa ggttgtaatt
     7321 tacccttggt caaaattttg tttggactcg tatcatattt atctccgttt tctgtcatct
     7381 tcgcgacatt agctgtaaca tcaaattgaa gcgggatatc gtccacgttg aattcgcctc
     7441 gcgtaataat agcaataata tctgccaata gagtatttgt atcccacgac cccatcccaa
     7501 aacgcaaggt atggcttcct gctgataata aactaccgct agtaaccttg atgggggcac
     7561 cggcaatata caagtaggcg ttgttccagt caattgcttt taatacatca ctgcttgata
     7621 catgttccgg caaattgatg atcgccacaa atgcaaatcc ttgtgcagtg atattcacca
     7681 aatcaagtac atttgttagt ttcacatggc tttcccaatg aaaacggacc gtgccacctt
     7741 tccaaggatc atttgaacca tcgttcaaat aagtagcagt caatttaggc aattggttag
     7801 ccccagttag ttggacaagc ttcccgccat tgactgcact acgtccacca tttggaggta
     7861 cgatattttc atacgtgtat ggatcataag aaatggcagg aggcgttgct ccaacagctt
     7921 ctgccgaagg tgaaaagata aatatactta aaacaaaaag cgaagtaaaa aagaaggagc
     7981 gaaaaaatga aaatttattt tgtagcaaca tgttcatcct ccttttcctt tcttctccat
     8041 aacaacaacc acgcaagaag ccataattct ataccaatga tcaaataaag aaaacggatc
     8101 ttttcttcat tcgtctccgg gaatacgcca tgttgattag attctttata aatcggtagc
     8161 tgtgtctgct gttttgatgc gccattgact gaaccacttg gatgcggaac tggacttaga
     8221 tcataaaatg taatcgtacc tgaacttttg gtatctgctt ggactggctc ttgccagctg
     8281 aagaacagca aacttgcaat aatccctccc aataagatcg atgttttcaa cgaaatcacc
     8341 tcatcatgtt gtttctcctt ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa
     8401 ccataacact agaactgcag caaccaatcc tgcataaagc catgggaaag attgttcttg
     8461 tactgcaaaa acggaatttt catttacttt ttgtgctttt tcttttttga tcgtaaagtt
     8521 ttgtgtaaat cgccattcat tactgtcgcc tgattttgct atgatttctg cgcggtaatc
     8581 gccggctttt gctttatcac cagctaaatc aagattcaag tgaaaattcg aatttggtgc
     8641 aactaaaaat tcattagacg tattttcaat ataaagctga ttttcataaa agatcttgct
     8701 cgtaaatatc aaattgctga taatcctagg ctgaggattt tggaaacgaa gctgaacagt
     8761 tggctttgcg ttgacctcgt ccaaatctcc tgataaaagt ttcagatccg gttctatcgt
     8821 ctcttgactt tcttgtaaaa cgactgcgag attataagca atacgatttt tgatttgtgc
     8881 tccttctgta gcattttctg tttcagcttc ttctttaggt gaaacatgaa tacccgctaa
     8941 tacacgtccc ttaaagcttt ttgctgggat tttcacttct ataggtatag ttgtttctgc
     9001 atgtgctgat acattgattt cctttttcgc aatgagcaaa acgtcacgga catcaaacgg
     9061 caatgtctca tctaacgttg ggtttttttg actataatcg atcgttccac catcactcgt
     9121 taccgccgga ttgacagaga cctcaaatgt tcgttcttca tctgaagaat tgctgataat
     9181 tacttctagt gtctgatttt gttccggtgc cactaataaa tcaaaataac cgattgcttt
     9241 atctatttgg ttctctggaa ataccggttt gattccaaag tctcctgctg aatcctttgc
     9301 ttggctgatt gacggaaaaa tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag
     9361 tcgtttactt tgattcattg tccaccacat cctttcaaca acaactgcgg taaaaaatcc
     9421 ttcacctata gtattttcac cgcagtttgt tgaatttatt tcatactcta tgctactggg
     9481 ccagccacga ggttccaagt caaatcagcc gtgaatgttc cagctgctgc tttcggcact
     9541 ttcagtgtga tatttgctgg atcataaaca gaaagccatg ttcccagacc ttgatctttt
     9601 gctgctgtaa atattggtgt cgctgcactt gctttactca acccagttac agtttgacta
     9661 gttggtccag tggattcgtt atctgacgta cttttaacag tggatgttcc aaaatctaat
     9721 tcagcgcctt gcaaaacttg tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc
     9781 cacccttgtg cttggccatc tgcacctaca cctcgacgat ctgatacttg gagataaggt
     9841 gtatcgtttt ttgaagcgtc tacttgatac gtttttgttc cgctttcaat ctcgtgtgta
     9901 ccaaatggca attcaggaac tacatccaat gttaaagaac ctgtctggcc agttgctgga
     9961 ttttccggat ctgctggctc tactggtttt gaaggatcag tagggttaag aggctcaacg
    10021 gtatttgttg gttcagtaaa ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa
    10081 gctggtgttg ccaagccgcc tagaagaagt gttgaaagta ataatgtccc aatcatgttc
    10141 tttttcataa taaatcactc ctttttctta gtgacctaag tatagtagag acagtatgca
    10201 aaaaatcctc atgaattcat ccgcttactt gaaattatga ccctttttgt ataaaagaga
    10261 aaaaaacaat aaaatcgttt acatgaatat tcttcctttt ttattaagta tgaaaaaata
    10321 tttcaatttt attttttcgt ttcaaattat tctattagtg acatccttga actataaaaa
    10381 taggagtacg gcatcatctg ccgcactcct atttttttcg tcatttattt cttaaataaa
    10441 acgattctca cgcaatttta tttttttgat atcttctact gtttgtgttc cagctaattg
    10501 catgaccatt tccaattctg ttttaaagaa atcaaacact tgatggactc ctgtacttcc
    10561 tcctaaagac aatccataaa taacgggtct gccaatagca acaagatcgg cacctgatgc
    10621 gattgctttg aaaacatgtt gtccacgtcg tacaccgctg tcaaacacga ttggtacacg
    10681 tttatctact gcttctgcca catattgcaa cgaatcaaac gcagcaggcc ccccatctaa
    10741 ttgacggcca ccatggttag atacccaaat tccaccagca ccagattcta atgatcgata
    10801 gacatcttct tctgattgaa cacctttcac gtagactggc aaatcagaat gagcagcgat
    10861 aaattctaca tcttttggac taagtttttg tttggaagat ttatatacag catccatcgt
    10921 ttggcctact cctgattgat aagcctgaac gatcggcatt ggcagcggga aggtaaaccc
    10981 gtttctgcgg tctgtttcac ggtttccgcc aactgttgca tccgctgtca atacgattgc
    11041 ttttgctcca ttacgcttgg ccacttcaag aatatccaaa ttgattccat tatctttgct
    11101 catataaaat tgaaaccatt gcggtgcttt ctccccgcct gcttctctta tctcttctaa
    11161 agtacaagaa gcgtaagaac ttgctgtata gatcgtccca aaatctgcta caccttttgc
    11221 agaagctttc tccgccttca catgagctaa tccatgagca gcaactggtg ccatgatgat
    11281 tggtgcagtc agcatttctt cgtcaaaatg tgtagttgta tcgggtaatt ctacatctct
    11341 taatacatga ggaataatca aacgatgatt gaatgcccgt tcattttctt gataagtaaa
    11401 taagtcgcca gcaccactgc tgatgtaacc ataaccacca gctggaatca cttgtgaagc
    11461 cgcgatttct aaatcttcca tattgataaa atctactatt ccttcatttg tacctgcttg
    11521 atatgttttt tccatctcaa tgccttctct ctataaaatt tgaatgatga acaatatgat
    11581 agcgtttaca ctttttatat tactcttcga cattgataca ggcaaataat ctgtttacat
    11641 ttcagccatt ccagatgtca ctactctgga agaaatgatt gtaataaaaa tagataaaaa
    11701 accgatggga ctttgacaaa agtagtgtca aagtcccttt ttttgaataa acggtgttca
    11761 aaagccattt tctcggtatt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
    11821 aaatttttct tactactcga aactaagcga ctttttcaca acctctcgat aaaacggtgt
    11881 tcaatcagct gaccctcggt attagctcaa aaaatggcaa aatatgagga actattttgc
    11941 catttgtctc tctaatactc aggtcagaac gttgacttca cagtctcttc cttaaacggt
    12001 gttcaaaaac ttgcatctgc ggtaataagc ccaaccaaac gaaaaatagg aggagctatt
    12061 tttgtttggc tgttcttatt acttgatgca gggcaggttt ttcacgacct ctgattcacg
    12121 gtgtttcttt ttcatcaaca tttttacgaa atactgtgga agactattga aataagtgtg
    12181 gattgtttct ttgtgtttca atcgtttctt gccattcttc atttaatttt ggattaccgt
    12241 tttcttcctt gaactcataa ggtcctaatt caaaaatccc agacagtatt tgattcacga
    12301 tttcatatag ttcagtgtga tcagacacat gattcttgaa gatcatctca ttatcaccac
    12361 gtttgaacct cacattcggg ctctcatgat gaacctctat gcatttttct ccttttttta
    12421 actgacggac ttctgaaaac atctcataaa tatcttctcc agtaaaacct tcagcaagcg
    12481 ccagactgtc cacaagaccg cctactgtca gttcagagat tattgcttga ttggaatcta
    12541 attttaatgt atagcttgtc gcttctacta cgccacatgc attgcaaccg ccttctgata
    12601 gttcgctttt tctaatcaat tgaacttctt tcattttctc ttcctcctct taattttcta
    12661 cttatgtatt tttatcacct gacataggtg actcctatca acgctatcat gtgctttttt
    12721 cctcagaaat cctatttatc cattccatct atcccttatt cttctacttt tctttctcta
    12781 gcatataaga gatacgcata gtattcaagc aaggattgtc gatataaaaa gaacaaatac
    12841 tgctattttg atttcttttg gctatattac atataaataa aatttatcat acctgctctt
    12901 tttgactata tcatcatctg acttctatac ttcgtttgta ttaaaactta ggaggaatca
    12961 ttcatgctta cagggttaaa cttattaaat aatccttttc taaataaagg aacagctttt
    13021 acaaaagaag aacgggcaaa atatggaatc actggtatgc ttccaagtac tgtccaaaca
    13081 ctggaacaac aatctgttca agcttatggt caatacttaa gcaaacaaac agatttggaa
    13141 aaacgtatct tcttaatgaa tctattcaat acgaatcgga cattgtttta taaattgatg
    13201 ggcgaacatt tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc
    13261 gaacaataca acgaaatttt cttgaaaccg caagatgctg catttctttc aatcgacgaa
    13321 ccagaaaaca tcaaagcaag tttgaagaac gcggcagatg gacgagacat tcgtttgatt
    13381 gttgtgactg atgcagaagg aatcttaggt atgggcgact ggggtgtgaa cggtgttgac
    13441 atcgctatcg gcaaattgat ggtctatact gctgctgctg gtattaatcc agcacaagtt
    13501 ttgcctgtat cgatcgacgc tggtactaat aatgaagaat tattgaataa tcccttatac
    13561 ttaggcaatc gtcatgctcg tgtagaaggc gaaacttact atgaattcat cgatcaattc
    13621 gttcaatcag ctaccgaatt attcccagaa ttgttattgc attgggaaga ttttggacga
    13681 ggaaatgctg cagcgatctt agaaaaatac gaagatcaaa tcactacttt caacgacgat
    13741 atccaaggta caggaattgt tgtcctagca ggtgtattag gcggattgaa tatatccggt
    13801 gaacaattga aagatcaaat aattttgaca tttggtgctg gtactgctgg tgtgggtatc
    13861 gcaaacatcc ttttagatga gatgatccgt caaggtgtac ctgaagaaga agcacgccaa
    13921 catttctatc aagtagataa acaaggttta ttgtttgaag atacagaagg attgactcct
    13981 ggtcaaatcc cattcgctcg caaacgctca gatttcacta atgctgatga attgacaaac
    14041 ttagaagcag ttgtaaaagc gatccatccg acaatcatga ttggtacttc tacccaacct
    14101 ggagcattca ctgaagaaat cgtgaaagaa atggctgctc atacaccacg tccgatcatc
    14161 atgcctttgt ctaatccaac aaaattggca gaagcaaaag caaaagattt aatagaatgg
    14221 acggacggaa aagcattagt tggaacaggt atccctgctg ctgatgttga atataatggt
    14281 gtaacttacc aaatcggtca agcaaataat gctttgatgt acccaggttt aggacttggt
    14341 ttgattgctt caacagctac acgagtaaat gctgaaatca tttctcaagc tagtcgagca
    14401 ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa
    14461 atcactgaat tttctcaaac gattgctgaa actgtcgcaa aatcagttgt cgcacaaaac
    14521 ttgaatcgtg aagaaatcac ggatatcaaa gaagcagttg aatctgctaa atgggttcct
    14581 gaatataaat ctttagaaga ttaagatcgt tatagtaaaa gaacatacag aaactttgta
    14641 tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagcg gccagccaac tcctgctctt
    14701 ttgtcgcttt tctttggctt ttttcatatg atacagaata agaaaggatc tgtaaaaatg
    14761 gcacaaaaaa caacaactgc aactgcagtt gaggaaaaga aaaaagaagg tttttggtcc
    14821 acaaaaatca ccggcgtaag tttgccactt tatttgatca tggtccttgt cctgatcatt
    14881 gcaatggctt tagggaaatt accgacgaat atgattggac caatcgcagt tttggttatt
    14941 ttcggtaatt tatttcattt tatcgggaca aaaatcccta tcgtcaaaag ttatttgggt
    15001 ggtggttccg tttttgcgat cttcgcttct gctgcgattg ctacatttgg gatcttacct
    15061 gaatatgtag tgaaatccac agaaaacttt gtcagcaata tgggcttttg ggacttttat
    15121 attgctgcct tgattactgg tagcatcctt ggtatgaacc gtaatctatt gatgaaagct
    15181 tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg tctccttttt tgccgtagga
    15241 ctcgtgggca tgcttatcgg aaatggtttt gcaaattccg ttttatacat ctcattacct
    15301 gcgatggctg gtggtgttgg agccggagcc gttcctttat caacgattta tgcaaatgtt
    15361 ttaggtacgg atgcttcttc tatcatttcc cgcctgattc ctgcgacagc aatgacaaat
    15421 gtcttagcta tcatcggtgc agcattagtt gctcgtgctg gacaatcaat gccaaaattc
    15481 aacggcaatg gtaaattaat ggaaaaaggc gatcttggcg atggaaaacc tcgaactgtg
    15541 aaaccttcca ttccacagtt aggtgttggt ttgatggtat cattgacttt cttcatctta
    15601 ggacaaattg gtaactattt tgttcctaaa gtccatgctt acgcatttat gattattatc
    15661 gttgtcatct gtaaaatcgc caacctatta ccagagtatt atgaagatgc agctatcatg
    15721 ttcaataact tgattgtcaa aaacttgaca gcagctgttc ttgccggtat cggtattgcg
    15781 ttgctgaatc tgaatgtatt agcttctgct ttgacatggc aatttgttgt cttatgtctg
    15841 acaagtgtta tcgcgatttc agttgtttca ggatttgtcg gtcgtttgtt tggtttatat
    15901 ccaattgaat caactattac tgctggctta tgcaacaact cgatgggcgg aacaggaaat
    15961 gtagccgtct tatctgcagc taatcggatg gaacttattg cttttgctca aatgggcaat
    16021 cgacttggtg gtgccatcgt tttgatcatc tctggtttct tgatgcaatt actttcttag
    16081 tcaaaaaaac aaaaatacct gatcatcaag tatcggcttg gtgatcgggt atttttttga
    16141 taaagattta caggattttc aaaaatcttt tttgcatttt atcggaatga taaccgatgt
    16201 gttcataaaa ttggtgtgct tctactctgt attctgcaga attaagcctt attgtcgtta
    16261 ttcctcgttt tttcgcttct tcttctacgc cagtcatcaa agcctttccg atgcctcttt
    16321 tttcagcata tttagacaca gcaagtgcca taatattgaa cattggatcg ctaaaaatcg
    16381 attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga tcttcataaa
    16441 ccaagaaaaa atttttctct ggctccttca gcagtttctt catttgtctc accgtaacgg
    16501 ttaatggtat atcataccct aattgatccg cattgatttc acgtagtgct tctgcatctt
    16561 ctaaacgcat ttcccgtaac atccaaatca ccagccaatt tctgattttt taaaatgaat
    16621 ttttttatca tagtatactc tttttttcat ttagaacaat atgtttcagt gattttgact
    16681 tgcgttagtc tagattctcc taatgtttgt aagatgacaa aggcattgta acaattgacc
    16741 aaaagaacca cgaggacaat tacttgaaaa ataaaggtca ctttttttgg agacaaaacc
    16801 ctgctaaatc ttgctcccca caaacctcca ataacagcag cggggataac ataaaacaac
    16861 atagataaat caaagcgcat aaagctactt gttaaagcaa tcgttaccaa ttttgccagt
    16921 tgagaaaaga aaatcgtaga tagagaatat agtgttgctt cttttattgg caatgcaaac
    16981 atcagcatca acaaagaaac attgactggt ccaccaccaa tacctaaaaa actagctaaa
    17041 aagcccagaa ccccccccac atataaggta ccaagtccat gacgtcaaat gaaattttgg
    17101 ctgatgatgc ttggtgtaga agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg
    17161 gatcagctgc acatgcttct cgttttcaaa taactgtaaa aaaacttcga atacaatgtt
    17221 gccgactact cctccaacaa cagctccact agatacccaa ccgacaatct gccaattcag
    17281 tgatttcccg cttgatactt gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac
    17341 tgttgagtaa aaagaaatcc ctgcaacaga atgggcgccg ataagatcaa gaatcggttt
    17401 gatcaacacg cctccaccca tcccggaaat agcgccaact gtgttcgcca gaacgatcac
    17461 cacaaaataa atcagaccaa tcatcttgta tttcctttct cttgtttata tcttttagcg
    17521 attgttactc ttttctttta ttatacagta aaagaaacaa acgaggcttc gaaagctttt
    17581 ctacttttta aggaaattta gtgctaaacg aaaagaggtt gtgaaaaagc tgtcctgcat
    17641 caagtaataa gaacagccaa acaaaaatag ctcctcataa ttttcgtttg gttgaactta
    17701 ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttca
    17761 gctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattgtga
    17821 cttgtgaccg aggagttggc aggagaacac cgtttaagga agagactgtg aagtcaacgt
    17881 tttgacctga ttattagaga aacaaatggc aaaatagttc ttcatatttc gccgtttttc
    17941 gagcgaatgc cgaggggcag ctgattgaac atcgtttatt tggaaaaagg gat
//
LOCUS       DACDSR010000052        17706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDSR010000052 DACDSR010000000
VERSION     DACDSR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.052"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            363..730
                     /locus_tag="IWK15_001775"
                     /pseudo
     CDS             363..730
                     /locus_tag="IWK15_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            910..1851
                     /locus_tag="IWK15_001776"
     CDS             910..1851
                     /locus_tag="IWK15_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9547360.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1920..2651
                     /locus_tag="IWK15_001777"
     CDS             1920..2651
                     /locus_tag="IWK15_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9547361.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2795..4975
                     /locus_tag="IWK15_001778"
     CDS             2795..4975
                     /locus_tag="IWK15_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9547362.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4995..5477
                     /locus_tag="IWK15_001779"
     CDS             4995..5477
                     /locus_tag="IWK15_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9547363.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5493..6254
                     /locus_tag="IWK15_001780"
     CDS             5493..6254
                     /locus_tag="IWK15_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9547364.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6385..7719
                     /locus_tag="IWK15_001781"
     CDS             6385..7719
                     /locus_tag="IWK15_001781"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9547365.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8147..8632
                     /locus_tag="IWK15_001782"
     CDS             8147..8632
                     /locus_tag="IWK15_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9547366.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8625..9308
                     /locus_tag="IWK15_001783"
     CDS             8625..9308
                     /locus_tag="IWK15_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9547367.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9434..12079
                     /gene="polA"
                     /locus_tag="IWK15_001784"
     CDS             9434..12079
                     /gene="polA"
                     /locus_tag="IWK15_001784"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9547368.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12124..12960
                     /gene="mutM"
                     /locus_tag="IWK15_001785"
     CDS             12124..12960
                     /gene="mutM"
                     /locus_tag="IWK15_001785"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9547369.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12924..13553
                     /locus_tag="IWK15_001786"
     CDS             12924..13553
                     /locus_tag="IWK15_001786"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9547370.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13605..13937
                     /locus_tag="IWK15_001787"
     CDS             13605..13937
                     /locus_tag="IWK15_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9547371.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14249..14740
                     /gene="nrdR"
                     /locus_tag="IWK15_001788"
     CDS             14249..14740
                     /gene="nrdR"
                     /locus_tag="IWK15_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9547372.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14763..16136
                     /locus_tag="IWK15_001789"
     CDS             14763..16136
                     /locus_tag="IWK15_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9547373.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPSMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16136..17062
                     /gene="dnaI"
                     /locus_tag="IWK15_001790"
     CDS             16136..17062
                     /gene="dnaI"
                     /locus_tag="IWK15_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9547374.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17191..17631
                     /locus_tag="IWK15_001791"
     CDS             17191..17631
                     /locus_tag="IWK15_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9547375.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         6025 a         3088 c         3891 g         4702 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gtaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      781 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      841 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      901 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      961 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
     1021 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
     1081 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
     1141 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
     1201 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
     1261 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
     1321 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1381 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1441 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1501 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1561 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1621 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1681 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1741 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1801 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1861 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1921 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1981 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     2041 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     2101 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     2161 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     2221 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     2281 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     2341 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2401 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2461 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2521 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2581 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2641 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2701 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2761 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2821 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2881 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2941 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     3001 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     3061 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     3121 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     3181 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     3241 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     3301 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3361 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3421 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3481 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3541 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3601 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3661 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3721 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3781 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3841 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3901 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3961 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     4021 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     4081 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     4141 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     4201 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     4261 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     4321 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4381 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4441 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4501 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4561 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4621 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4681 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4741 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4801 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4861 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4921 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4981 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     5041 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     5101 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     5161 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     5221 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     5281 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     5341 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5401 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5461 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5521 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5581 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5641 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5701 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5761 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5821 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5881 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5941 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     6001 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     6061 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     6121 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     6181 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     6241 tttcccgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     6301 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6361 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6421 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6481 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6541 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6601 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6661 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6721 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6781 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6841 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6901 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6961 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     7021 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     7081 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     7141 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     7201 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     7261 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     7321 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7381 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7441 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7501 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7561 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7621 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7681 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7741 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7801 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7861 gagaagctgt ttttcttggc tgttcttatt acttgatgca gggcagcttt ttcacgacct
     7921 cagataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa atgaaaaata
     7981 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     8041 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     8101 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8161 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8221 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8281 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8341 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8401 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8461 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8521 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8581 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8641 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8701 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8761 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8821 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8881 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8941 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     9001 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     9061 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9121 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9181 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9241 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9301 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9361 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9421 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9481 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9541 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9601 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9661 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9721 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9781 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9841 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9901 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9961 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
    10021 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
    10081 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10141 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10201 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10261 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10321 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10381 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10441 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10501 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10561 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10621 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10681 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10741 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10801 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagata aaaaccaaga
    10861 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10921 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10981 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    11041 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    11101 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11161 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11221 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11281 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11341 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11401 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11461 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11521 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11581 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11641 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11701 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11761 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11821 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11881 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11941 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    12001 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    12061 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12121 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12181 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12241 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12301 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12361 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12421 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12481 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12541 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12601 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12661 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12721 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12781 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12841 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12901 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12961 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    13021 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    13081 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13141 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13201 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13261 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13321 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13381 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13441 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13501 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13561 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13621 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13681 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13741 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13801 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13861 gtgaccacat ttccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13921 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13981 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    14041 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    14101 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14161 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14221 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14281 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14341 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14401 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14461 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14521 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14581 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14641 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14701 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14761 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14821 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14881 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14941 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    15001 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    15061 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15121 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15181 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15241 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15301 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15361 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15421 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15481 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15541 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15601 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15661 ttcagctgat aaaagaagca cagagcattc catcgatgaa atatctggaa gcaatcaagt
    15721 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15781 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15841 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15901 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15961 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    16021 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    16081 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16141 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16201 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16261 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16321 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16381 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16441 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16501 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16561 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16621 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16681 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16741 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16801 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16861 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16921 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16981 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    17041 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    17101 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17161 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17221 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17281 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17341 atatttgtcg gaacgattga aagtattact caagtagaag cagaagaaga aacgcttcag
    17401 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17461 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17521 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17581 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17641 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17701 tatggg
//
LOCUS       DACDSR010000053        16491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDSR010000053 DACDSR010000000
VERSION     DACDSR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.053"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            39..1451
                     /locus_tag="IWK15_001792"
     CDS             39..1451
                     /locus_tag="IWK15_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9547376.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="IWK15_001793"
     CDS             1800..2432
                     /locus_tag="IWK15_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9547377.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="IWK15_001794"
     CDS             3020..4459
                     /locus_tag="IWK15_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9547378.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="IWK15_001795"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="IWK15_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5221..5436
                     /locus_tag="IWK15_001796"
     CDS             5221..5436
                     /locus_tag="IWK15_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547379.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5653..5909)
                     /locus_tag="IWK15_001797"
                     /pseudo
     CDS             complement(5653..5909)
                     /locus_tag="IWK15_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6096..7007)
                     /locus_tag="IWK15_001798"
     CDS             complement(6096..7007)
                     /locus_tag="IWK15_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9547380.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7026..8030)
                     /locus_tag="IWK15_001799"
     CDS             complement(7026..8030)
                     /locus_tag="IWK15_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9547381.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8027..10150)
                     /locus_tag="IWK15_001800"
     CDS             complement(8027..10150)
                     /locus_tag="IWK15_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9547382.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10155..12602)
                     /locus_tag="IWK15_001801"
     CDS             complement(10155..12602)
                     /locus_tag="IWK15_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9547383.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12589..12978)
                     /locus_tag="IWK15_001802"
     CDS             complement(12589..12978)
                     /locus_tag="IWK15_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9547384.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13038..13541)
                     /locus_tag="IWK15_001803"
     CDS             complement(13038..13541)
                     /locus_tag="IWK15_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9547385.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13554..13778)
                     /locus_tag="IWK15_001804"
     CDS             complement(13554..13778)
                     /locus_tag="IWK15_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547386.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13882..15792)
                     /gene="ltrA"
                     /locus_tag="IWK15_001805"
     CDS             complement(13882..15792)
                     /gene="ltrA"
                     /locus_tag="IWK15_001805"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9547387.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4912 a         3271 c         2939 g         5369 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5281 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5341 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5401 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5461 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5521 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5581 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5641 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5701 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5761 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5821 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5881 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     5941 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6001 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6061 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     6121 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     6181 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     6241 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     6301 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     6361 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     6421 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     6481 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     6541 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     6601 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     6661 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     6721 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     6781 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     6841 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     6901 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     6961 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     7021 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     7081 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     7141 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     7201 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     7261 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     7321 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     7381 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     7441 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     7501 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     7561 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     7621 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     7681 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     7741 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     7801 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     7861 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     7921 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     7981 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     8041 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     8101 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     8161 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     8221 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     8281 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     8341 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     8401 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     8461 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     8521 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     8581 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     8641 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     8701 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     8761 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     8821 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     8881 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     8941 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     9001 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     9061 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     9121 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     9181 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     9241 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     9301 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     9361 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     9421 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     9481 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     9541 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     9601 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     9661 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     9721 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     9781 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     9841 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     9901 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     9961 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
    10021 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
    10081 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
    10141 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
    10201 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
    10261 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
    10321 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
    10381 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
    10441 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
    10501 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
    10561 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
    10621 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
    10681 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
    10741 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
    10801 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
    10861 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
    10921 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
    10981 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
    11041 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc
    11101 ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga
    11161 ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg
    11221 gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc
    11281 gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca
    11341 aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc
    11401 ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt
    11461 tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct
    11521 actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga
    11581 tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt
    11641 tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt cttattgcga
    11701 acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg
    11761 aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc
    11821 acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat
    11881 aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc
    11941 accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt
    12001 gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt
    12061 ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg
    12121 cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag
    12181 acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca
    12241 tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc
    12301 atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc
    12361 ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt
    12421 tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg
    12481 acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag
    12541 gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac
    12601 atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac
    12661 tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa
    12721 tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg
    12781 tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa
    12841 tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa
    12901 aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta
    12961 gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact
    13021 acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa
    13081 ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt
    13141 tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata
    13201 tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa
    13261 ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc
    13321 tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag
    13381 ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct
    13441 ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta
    13501 tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca
    13561 ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc
    13621 aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta
    13681 aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg ggcattggtt
    13741 aaaaaccatt gatatatgga taggtaagtt cctgatatta taaatatctc aaacttttcc
    13801 ccccattcac acggagcgtg cgactttcac cgcactccgc gttccatcgt gtttatattg
    13861 aaaagtattt atgtagtaga cctatatcaa gtttaattct tgattgccag ctaatactct
    13921 atatttattt agtcttatga gctggacttt atttagcttc aatagagcta tatatttttg
    13981 tattgtttct tctgttttag cgtgtattag acggtgtacc gcctctttta ccaaaataag
    14041 atttttataa tcgtcatttc caccagctcc agttggtaca atatgatgac aatgtatttc
    14101 attagcttgt tgaaattctt ccagagttat agcacatttt ccatgttgag cagaaaatag
    14161 cgaaattcga ttgtctgcgt attctattgt gcgattttcc aaagataatt tcattaactg
    14221 gtacatcagt aatttatcaa cacttagatt ggtgtggata aattttcttc cctctagagt
    14281 gtagcaattc gctaaataca cctttgccat tggttttctg tgttggatat atccaacagg
    14341 gaaaataggt tcgccctcgc cagcaacata ccgaagcatt ttagatttac catatcttgt
    14401 tgcctccttt gtcgtaagtt gtctgcctgt ttttctcaat ctgccccttt tgtttgtacc
    14461 taatctgtta gtgaagatag ccattacagc tctattcagt ttcatacaat ccagatttac
    14521 attcgttgca atctgataat aattttgcat acctaatacc attgaattat aaagtcttgt
    14581 ttcttctaat tccgttttcc cttttgaggg agaagcaatt ttctttgcct gtgataccag
    14641 cttgtctttt tggtttttga attgtttatc acttatatgt gatttaacaa ctaacttctt
    14701 tcttctcggt atcattttaa tcttgaaacc aagaaagtcg gagtatcttt ttctaacatt
    14761 tacaattctt gttttttctt gtgatacttc caattttaat cgttgttcaa tccattgagt
    14821 gattgctatt ttggcttttt cagcactttc cttgtagcgg cagaaaactc taaaatcatc
    14881 tgcataacga accatataca tttcctttaa ctttgttttc ttcatttctt tgtaagcgtt
    14941 gcttttacat gggctaccgc ttttattgat atgaaccaca tagttttttg taacaggatt
    15001 ttcctgccat tggctttcta cccaatggtc taactcatta agaacaatgt ttgctagtag
    15061 tggggatatt attccccctt gcggagttcc tttctctgga tatgttattg taccgttagt
    15121 cattttgatt ggagctttta atatcctttt taaaatgaat atcaagtgct tgtcccgtat
    15181 tcccatagcc catatctgtt ttatcagctt ggaatggttc acgttgtcaa agaaaccctt
    15241 aatatcaaat tcaatcacat aatgtagctg gctgatttgt aggcggttat aggttgcttt
    15301 tattgcattt tcaactgacc tattaggtct aaaaccatag ctgttttcac taaatttagc
    15361 ttcgcagatt ggttctaata cttgttttat acattgttgt ataagcctgt cccacataca
    15421 aggaatacct agaggtctgg ttttaccatt tggttttggt atttcttttc gcctcactgg
    15481 cttaggtcga tacccatgtg gactgcctgc aacaatgtat cttacttttt tgacaactgt
    15541 ttctgctggc aatttgccaa tatctttaat tattgtaccg tctgttcctg ctgttttact
    15601 gcctgcattt gctttgatat ttcgatatgc cagtaatata ttttctctcg ataggataat
    15661 atccataaga tgagtgaaaa tatctccatt cttacttttg gcatataaat catcaaaggt
    15721 gttttgcatt tcgtaatatt cagcgtgtct taaatcttct acacacaatg tttcattttt
    15781 atttttaggc ataaggcttc actcctttac ttgaaattga gtgagctttt ttatcttact
    15841 cgtaatcctt attgaaacaa cataatactt ttctaatata tacacgactt gagggcattc
    15901 ctccattctc attacaagaa tttcttaggt tcgccctctg cttttcagca ttagttcaac
    15961 tgcttattgt tcttcgtcag tttattctct tgatgagaac ccaatacctt tcctcgttcc
    16021 gataatctta tctatacata tatatcctta ggtcactagc tataagcctg ctggcttgat
    16081 accgcctgta acgttatatg gcatttcata acctatattc ttactttgcc acaccagcta
    16141 cgatttctcg tacctttgca tttctacaaa gttcaaattt agacccgtac attcgctgtt
    16201 tcgtcagttc cttatagaac attctaacca tagatatttt atagaccccc gacctgttgg
    16261 atacgctacc cacctctgga tagttttcga tagaagatat atgttcatca actattacgg
    16321 tatctttcag ccgtctttac cgagcttcaa acattctgag tttcctcata caatgcttgt
    16381 cggaggctca gggcaggcgt ttcagagcgt tacttcatca ttccacctgt cagattatca
    16441 gttctcctaa taaccaaact ttttcgttct tttattagtg ccttagcttt t
//
LOCUS       DACDSR010000054        16427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDSR010000054 DACDSR010000000
VERSION     DACDSR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.054"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..590)
                     /locus_tag="IWK15_001806"
     CDS             complement(27..590)
                     /locus_tag="IWK15_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9547388.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(605..1648)
                     /locus_tag="IWK15_001807"
     CDS             complement(605..1648)
                     /locus_tag="IWK15_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547389.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1781..2185)
                     /locus_tag="IWK15_001808"
     CDS             complement(1781..2185)
                     /locus_tag="IWK15_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9547390.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2418..3053)
                     /locus_tag="IWK15_001809"
     CDS             complement(2418..3053)
                     /locus_tag="IWK15_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9547391.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3319..4371)
                     /locus_tag="IWK15_001810"
     CDS             complement(3319..4371)
                     /locus_tag="IWK15_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9547392.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4384..5322)
                     /locus_tag="IWK15_001811"
     CDS             complement(4384..5322)
                     /locus_tag="IWK15_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9547393.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5773..6423)
                     /locus_tag="IWK15_001812"
     CDS             complement(5773..6423)
                     /locus_tag="IWK15_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547394.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(6523..7032)
                     /locus_tag="IWK15_001813"
     CDS             complement(6523..7032)
                     /locus_tag="IWK15_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9547395.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(7233..8102)
                     /locus_tag="IWK15_001814"
     CDS             complement(7233..8102)
                     /locus_tag="IWK15_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9547396.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(8122..9243)
                     /locus_tag="IWK15_001815"
     CDS             complement(8122..9243)
                     /locus_tag="IWK15_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9547397.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(9245..9640)
                     /locus_tag="IWK15_001816"
     CDS             complement(9245..9640)
                     /locus_tag="IWK15_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9547398.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(9659..10456)
                     /locus_tag="IWK15_001817"
     CDS             complement(9659..10456)
                     /locus_tag="IWK15_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9547399.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(10458..11246)
                     /locus_tag="IWK15_001818"
     CDS             complement(10458..11246)
                     /locus_tag="IWK15_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9547400.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(11260..11736)
                     /locus_tag="IWK15_001819"
     CDS             complement(11260..11736)
                     /locus_tag="IWK15_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9547401.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(11808..14465)
                     /locus_tag="IWK15_001820"
     CDS             complement(11808..14465)
                     /locus_tag="IWK15_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9547402.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(14484..14888)
                     /locus_tag="IWK15_001821"
     CDS             complement(14484..14888)
                     /locus_tag="IWK15_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9547403.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(15120..15377)
                     /locus_tag="IWK15_001822"
     CDS             complement(15120..15377)
                     /locus_tag="IWK15_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9547404.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(15502..15834)
                     /locus_tag="IWK15_001823"
     CDS             complement(15502..15834)
                     /locus_tag="IWK15_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547405.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         5128 a         3376 c         2414 g         5509 t
ORIGIN      
        1 taaaatggat gctaggtccg ttatatttac tcaaaattga ttctacgaat agatcttctt
       61 atcatttcta ttttagtagt catgcgttgt tgttcttcta aaggtggtaa tggtataagt
      121 agtttagata aatttgtacc atttacattt ggttgcccag ttccgtaaga aattttttct
      181 aataacttcc aatataatgg cgagtctaag aagcagtcta cgtattctga taataatttc
      241 tcaactaacc taattcgaat caagtaagaa gcaaaaacag attcctcact tatttcctta
      301 accaagaaac tttttcccat agttcctcct gtccttgcta tcaaaatgtc attttcttcc
      361 aatcttaaat caactagttt agaattacta atatcacagt aaggcacact ggaccaattt
      421 acacgtccct cttgtatatc tgtgattctc aaaaacttaa cattgccttg ttttttcgcc
      481 gaatctgtat agccatattg aatcttagta gaaatacttc ctaaataaac ccatagtcga
      541 acatcgctag atgatttttc ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt
      601 ccgactattt tttagttgca gcaatctttt tgggacgctt atttgcatga agcaacgttg
      661 ctaagtctag taataaaaca ttggctaaca caggattggc tttctcgcga tacgctgcgg
      721 cgccactttc aaacaatact gctagacgat cttctacaac aacgatttgt tccaaatacg
      781 gtggaaattt gattcgtttt aggatccggc taggagagtc ccgctcggac actaagagtt
      841 ccgaggtata aggaccataa gaacgactgg ctaagagata atggccataa aaagccacac
      901 cttgaatctt atcagatgtt tttctgctca ttgtcacggt tggttggatt ttgtctttgc
      961 ggcgaattgc tgttttcatt tcagctatcg tcggcaatag atagaaagtc aactgtcctt
     1021 caccttttag tgtgaagttg ccagatacca gttgatcatt gttttgagtc aaatacgatg
     1081 cttgtggaat ctctgagaga tcagttgtca gccaataagc gattggctgt cccgtctctt
     1141 cagatgtatc tgctaagata tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc
     1201 ctgtgattgt caaccataaa agttcccgtt ttgtatcata tactaagccc cctacatgtg
     1261 gccgtttagg taaaatcagc agtttgatcg gctgtcctgt ttctctatcc agtacatgaa
     1321 taatagaatg atgctgatgg tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa
     1381 aagccaaccc ttgcggcgtc atccattgac aaacctcatt ttcgccggtt tccggcacca
     1441 ccattcttgt ttctgttaat ccaggaatgt tatattgttt tgcttcagcc ttttcccaag
     1501 ctgcttgaac aataggatac cgttttaact ttttttcaat atcctgatgt atccgttgct
     1561 gcttccaatg cttaaacttt aggacatagt aaacaagaag gccaatgacc agcaacacta
     1621 aaatcattat tgctacccgc tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg
     1681 tccatatacc gctattaatt accgtaacta catgtttctt ttactagcgt ttctttatag
     1741 gatctttcat aatctcattt atagtaacat taagaatccc ttattctact atagtcactt
     1801 ccacatcaat attgcctctt gttgctttag agtatgggca aaatgcgtgg gcttcttctg
     1861 cgattttctc tgcttggtcc ttctcaagtc ctggcaaaga aacttctaaa acgaccccga
     1921 tttggtagcc cccatcttct aattgaaata aagatacccg tgctttgact tcagaatcag
     1981 actcaagatt attttcttcc agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc
     2041 cagccgcaaa aagttgttcc ggatttgtcc cttcactatg gattcctggg cggtcgatct
     2101 tcatgtgcat acttccgttt ggtgcctcaa cttcgccatc acggcctcca cgattgatca
     2161 ttgctgtttc gtacattttt ttcattgaga tcatcctttc tttcgtcgtt ctatattaag
     2221 tgtaacgagt attttttaat ctgacaaagg taatgcttgc attcggcgac atcagttaga
     2281 caacatcgat gatcagtgat agaagcagtt tatctataca aaaaaagaag ggttctatga
     2341 tatttggtgt ccttactcgc cttgagtaac aacaccaaat atcatagaac cacttaatca
     2401 gcttcacact gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa
     2461 tagttaaaga aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga
     2521 ctaatcgacg gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt
     2581 aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt
     2641 aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa
     2701 gtcgatagat tacaagagct ttcattcgta ttgatactga ataagccagt aaaaatacaa
     2761 tcacctatcc catataaacc aactgttgca acaacaagta ttgcaagtgt ttttgatgtt
     2821 ccaacaaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact
     2881 gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg
     2941 gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca
     3001 ctcatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc
     3061 ccctactttt tagtgattat tcgatacatc caacaatcaa attttttctc attttaacac
     3121 atatttttat tctagttgtc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca
     3181 ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa
     3241 aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa
     3301 atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt
     3361 aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca
     3421 acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag
     3481 tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg
     3541 aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag
     3601 attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt
     3661 aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc
     3721 accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga
     3781 gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt
     3841 agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa
     3901 ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac
     3961 tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat
     4021 cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg
     4081 tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat
     4141 cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg
     4201 aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa
     4261 aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc
     4321 gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct
     4381 cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca
     4441 ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc
     4501 gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc
     4561 aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc
     4621 aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg
     4681 agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt
     4741 cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg
     4801 agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc
     4861 atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc
     4921 ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt
     4981 ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt
     5041 gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat
     5101 aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc
     5161 ttctacctct ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg
     5221 ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca tcaacccaaa
     5281 gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt
     5341 ctttctatta tactcatttg agaatctttt cacaacctcg ttttgttatt cttctcttat
     5401 ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat
     5461 agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat
     5521 agaaaaaatc tcactcctct gattataaaa gagggggcat taacctcgtg tatactaaaa
     5581 ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat
     5641 gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta
     5701 tttaaaaact tctttcccag attttcttcc atttttacag ttataactag tattatcatg
     5761 aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat
     5821 tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat
     5881 ccaagcccag tctaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc
     5941 atattccgtc cttaaaatta tattttcagt gccagacaag tgatttatat tgagttgata
     6001 aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat
     6061 tccatcagct ctatgtgtta catacaaata attgtatgac ctcatttcac ctaatgaatc
     6121 aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt
     6181 taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct
     6241 actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt
     6301 cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct
     6361 acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt
     6421 cataaaacca atccagctcc tcgattaaga taatatttta atctcataca aaaactagct
     6481 ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt
     6541 ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt
     6601 tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc
     6661 ctcagcaaaa acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt
     6721 tagcaacaca ttgttgatat atttgatttc cttgcttttt tgcggcgtgt ctactatcgg
     6781 cttatctttt tgcggaccac gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac
     6841 ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg
     6901 tactttggtc agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa
     6961 agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc
     7021 attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc
     7081 ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgataag gcgctgggta
     7141 tcatgagtga atgatattgc tgaatacaaa tagtccgacc acattaagtt gatcggactt
     7201 cataagcatt ttattgacta atactttttt gcttaagaat tgattctaac atcttttgct
     7261 aaagttaagc atcctatagt tcctgcatta tctgctaact taggtgtgac aatgtattct
     7321 tcgatatttg gcgtatcgac atagccgtta attagttcta aaaaacgtct ttgtactttc
     7381 tttttcagat gttcttgttg catgacacca ccaccaaaaa ttataatatc tggtgaaagc
     7441 atcagggtcg tattacaggc acattgtgca atataatccg cctcgatttc ccagaacgga
     7501 tcttctatag aaagatcttg tcccttggtt cccgtacgca tctcgatagc gggtccagat
     7561 gccaaccctt cgagacaatc atgatgaaaa ggacaagtac cagagaaagt atctcctgaa
     7621 tgtctcttaa ctaacgtatg ccccatttct ggatggctaa acccttcaac aaaaatccca
     7681 tcttgtatag ctcctccacc aattccagtt ccaacagtgt aataaacaac actggataaa
     7741 ccttttccat gacctgaaac atactccccg taacaagcag cattcacatc ggttgtccag
     7801 gcaattggaa tttcgaaatg cttattcaac gttccaacaa aatcaaaatt tttccaggca
     7861 gtttttggag tagaggtgat atgtccatag gtctttgatt cacgatggat atcaatcggt
     7921 ccaaatgacc ctactccaat gctttctaat tgttctttat agtgattaaa gaaatcaatt
     7981 accaaactca ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc taaaaccttt
     8041 aaatcatcat tgccaatggc acaaacaaat ttagtcccac ctgcttctat tgagccatac
     8101 atttcttact ccaccgtcct tttatctagg aattcgttct tccctgtcta atagtttttc
     8161 gaaaaactca tgtggttcct tttggtacca ataggcaaca gaagaaatat cgtcttgacg
     8221 ctcataaagc ttgatgtcat catttcctag ggtttggaaa gtaacacgta aattttcttt
     8281 gaaactaatg gggtcgggta aatgccagcg gtacaaccca tgcatcggta aagcatcatc
     8341 gccaaaacca tgaactggat ttggattagg ttttccagca ctaaaataat cccgggtgtg
     8401 atctctttta gattgaaaag gatagcccat aaatagcgta ttaaaatttt gagcatatgc
     8461 cctttctcca aagttctttt tatggaaagc ccaagcgccg ccaaaataat cttctgctcc
     8521 tgtagatgtt attgtaggga aatcttcatc tccatctata taaaacttaa attctccttc
     8581 cccccaccag tatctttcca atgcgcacaa agcaaagtag gttcctacat aatacccagt
     8641 gcctttgatt ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt
     8701 ccatttagca tgaaaataca tcgcatcatc cggtacttca gtcaatgcat aattaaatgt
     8761 ataaaaaatg tgcttaataa actcaggatg ttcattcgtt aaagtaatct tagctttttt
     8821 tcgaaatggc atctcaaaat agcaattcat ccctgatgtt ggattcacta cgattggtaa
     8881 ggaattcact tcgcatcttg tcccaaaccc attacaaaaa aaatctccta atggactttc
     8941 tacagaagga gtggattcat catcccaata aatacgcaaa attaaatcac gtaagacgaa
     9001 actcccagcg attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc
     9061 ttccatttct gctaaagtaa ttgtctctcc agcatttatc ggaacagaag gtgacccttt
     9121 tctcccaatt cctagatgac tcgctgtcat tgccgctttc cctttttcac catttttatt
     9181 ttctggtgaa atagttctac tttcctctcc cttgtggagc catatattat ttaaacaatt
     9241 cattttattc gtcctctatc ctttcttcac ttttttgatt attcatatag atgatttgtt
     9301 gttgtgcctc cgcaataatt gctccaatat ctaaattatc atttcctaac tgcaaagcga
     9361 ttgacattgc caagggaaga ttcattcctg caattacgtg cactctgtca gaaatatagg
     9421 taaaaaactt ttgatttaca cttcctccag ccatgtcagt taaaacaatt atttcgtctg
     9481 tagtatccac taactcttta atttgttcat ctatatcatc aatttcttcg acatatgcac
     9541 ataagctact aatttgatta tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct
     9601 cagctagtat tgaatggcta gcaatgatca atcttctcat aattcaaatc cttttccttt
     9661 acgctaatat tccaaatgca gccataacca tcgcaaaaac aataacgagt agaatcaaaa
     9721 tggtcatact gatttttttc ttactgagta gataatatgc tgcagcagta ataattactg
     9781 gaattaatgt tggtaaaatt ttgtccaaca ttggttggat tttcatacta acttcaccaa
     9841 aagtgaattt aagtggcgtt gaaacactga taactgaagc aattaatgat ccaactactg
     9901 ttagccccaa tactgaagca gcttcagtta aataattaat cttttcaccg tattcagtaa
     9961 caatcttcat tccctgctta taaccaaatt caaagcctct cattcggaaa aagaagatta
    10021 ccatgattaa aatgaaccat aaaaagattc ctacaggatt attttctaaa gccatataag
    10081 ccgcaattga tcccatgatg gttgggatca tcgtcatcaa aagagagtcc ccaataccag
    10141 caattggacc catcgtacca actttaaagt cattaatcgc atcttttgct tctaatcctt
    10201 gcttttcctc cattgcaaca caagcaccta aaataatggc tgccagccaa ggctgtgtat
    10261 tgtaatactg aaagtgatta tttaaagctt cgatgtattc atcatcatct ttgtataatt
    10321 ttcttaaaat aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa
    10381 aggtacaaac tgacatcaac caacgatagt tcgctttcct aagatccttt ttcgttactt
    10441 gatatttttc gctcatttta ttcatcctcc ccaataaacc caacatcaga agctcccgca
    10501 gaagcagtga tttgtgcatc tttttcagct ctatgataca cattaaatgc taaagcgaat
    10561 cctatgatcg ccgctccaat aataggtacc ttcaaaaaag cggctaagaa aaatccaaca
    10621 agtaaataac taatgtatcg acctgtaggc ataaagcgta acaacatcgc aataccaaca
    10681 gcaggcaaca tttttccggc taatgttagc ccatccataa accaattagg taaaacatcc
    10741 aaaacgtaat taacagcaga ctgtcctaaa gtaacggcaa taaatactgg aattgctgca
    10801 gataaaccaa agaaaattgg ccccaaccac aaaacactat tcattttctt aaattctcgt
    10861 ttattcgcat attcctgtga tttgcgaaca acaaaaccat ttaagatttt gacaattaca
    10921 tctagttgca cccctaacat acctacaggt agtcctattg ctaatccggc ttctactcca
    10981 agctggctac caaatgccgt tgcaataatt gcgcacgttc catagtccgg tacagatgag
    11041 ccaccaaaac cggcaactcc taatgacatc aattgaactg taccgccaac tatcaaacct
    11101 gtttttatat cacccatcaa aattcctgtg aataatcccc aaaaaactgc gtaatttaca
    11161 aagatcatcg gaatcccata ataatcaaca tgtttaataa atgctaatat agtcagcata
    11221 ataatttgaa aaatagtaaa atgcattatc cttactcctt tatcctaatt ttgattgaat
    11281 ttcctgtgcc ttatccgccg gaacaaattg tgtaacaatt tctacaccag aatccaacaa
    11341 ttttttatac gtgctgatat tttcatctga tgcataggct ctagaagata gctgtaatgc
    11401 attatcttta acaacagtac cgccaacaat tagtcggtca attctaatac cgttatctac
    11461 taaatgaagg attgtttccg gctctttcac gatgacaaat actttttcct gatcgtattt
    11521 ccccttttta aaattcgcaa tcgctgtttc ttcagtgata attgatacag ccattccagc
    11581 aggtctagct aaacgcatac ttgatttttg gatgtcgtta tttgcgacag aatcatcgat
    11641 caccatcact ctgtttgctt tagacattgg tgcccattgc gtgacaataa tcccatgcaa
    11701 taaacggtta tcaactctcg acataacaat actcataaat acttcctcct ttatctttca
    11761 ttttcttaca agcaattacc atgccaaaac aaaaagaact tgataaatca agctcttttt
    11821 acataatcat aaacgtattt aacctcatga atcgggatgt cgacactata ataatgttca
    11881 acgacactga aagattgctt aaaatgtgtc tgaaattttt gaaattcctc actcgtttca
    11941 ttgaaataaa tttcatcttc caaagtattt tgttttacta atcgttcaat taagcaacta
    12001 atgtgaacat ataatccgaa acacgtatta ttatcaaatg aaattcctag atcattttgt
    12061 aagatgtata tagcatctga aacccgttct aataatttag tcggatttaa tatggtcaat
    12121 tgctccatta aattcgttag actaaaattc ttcagaatat tattgttcaa aactttcagt
    12181 tcattatcat taaagtgtct attcaattga ttggagaatt tacttatatt cattcctaaa
    12241 atcatatctt caattgaaaa gaattctaca tcttctagat gtggatttag tgttccaaca
    12301 ataaaaagaa tattgttctc tttacgaagc tccgataaat agttttcatt cactaaagag
    12361 taatagtcac acgtttctat gacaagatca gtatcccttg gaaagctatc ttcaagtatc
    12421 ttttttaatt taaacgaagt tcctaatccg cttgcacaag aacagacaat catatccttc
    12481 ttaatatttg atgattcata aatataatcc acattttgat tttcttttac ttgttcaaga
    12541 atctgtttaa gcggtaaatc taataatatc tgatttgcaa catcaatagc cattcttgtt
    12601 gaaacattgt ttagtagtcc gaattttttt tgagcgtcta gttccaaatt tagataaatt
    12661 ttctccagtg aacccatatc aacaagtaaa ataatttctt catatccggt acactcttgt
    12721 aagtatctat taattttgtc tgcaatctca ctacttgaag tcattagtgg catatcaatg
    12781 gcgtcgtata tatatctctc aacgatccta ttagcagtac ttgctataca ggttgccgtt
    12841 agatctccat gagccaaaat cagtgctaaa cgtttttgct gaaattcttt actacacatt
    12901 aacttaaata aaataaccaa aacatttttt tgaatttttg ttaaataaat atcccctatt
    12961 tgaataaaac gattgactaa ttgaacttct cgactacacc aactattaat atcattgatg
    13021 aaggaatata atttatcgtc cgatgttaat tggatttgat aaatccaaga attcaaaaag
    13081 atagacgtta tttgaagata ataatagtca aattctattt gaaaatctct ctctatttcc
    13141 ttaacatact gtctaaatcg aatctcttga taggtgggat atgttaaagc tgaattattt
    13201 tggatatcaa cattcttttg tctaaaatat ctatcatatt tctcaaaaag ctgctgctta
    13261 tcagtgtctt tcttccacgt ttgataaaga tactctataa gttcgttgat attaaaaact
    13321 tcgtctactt tttccaaatc atccatttga atatatttta attcattctg tcttttcata
    13381 accttcatgc cgttgaaatt cacccattgt ggtaaatgat acatctgaat ataaagttct
    13441 tccaaatcat ctaagaaata aaaggcattg gcacaggtca attgaatgat atttttcaaa
    13501 ttgccaacat tttcactaaa gcgatagttt aataaaaaat ctaataattg ttgagatatc
    13561 agtattttct ttgctagtct ttcttcttct gatttaaaaa atatcttgat taactgtttt
    13621 ctttcataaa gagaacgact gtctaatgtt ggaagtgtta gctttacagg tattcttctc
    13681 aacaaagttg ataatagttt atcctcaggt ggttctgatg ttgcaaatat aatccagcaa
    13741 cttgatgtgt accagttttc attatcaccc atccgatgaa atttcctcgt atccataaac
    13801 tgaaaaagtt tctcctgaca ttcattgctc aatgcatgaa tttcatctaa aaataagact
    13861 ccattattag ctaattctgc taaacccttt cttccatctt cggctcctgt atatgcacct
    13921 tttacatgcc caaaaaaatt ggacaagaaa agttctggat tattggcata ttcactacaa
    13981 ttaactgaaa tgaaagatgc cttctcttct atataattat tggccaacaa atccttatac
    14041 acataatttg ctaaatagct ttttccagtt ccagtctcgc catgaagtaa aatgggtaag
    14101 ccatttggcg gatagtagat tgctgcttta atttgagcaa ttacatttcg cagacttcct
    14161 tgacttccga taaccctttc tattccaacc ctaaaagagc tatcatacca tttttccgct
    14221 tcctctgaag agctaaaaat ttgttgattt ggaactttct ccggtaaaga aaagaagatt
    14281 actggacgag tattgatttt tccaaattct ccatctttta cgaattcatt caaatattga
    14341 gagacaatat tacgactaac atttaatttt tcagatattt tctgtgctgt attgttcccc
    14401 catttatttt taatctcaga gttctctatg acataattga caatgcgttc ttttaattct
    14461 tccatcacag cacctcctcg acactattcg aattttgccg acagtatttt tccataaata
    14521 tgacttgcta aaaaggcttt gtttagatca ttcaattgga acgttgcttg ttttgcttga
    14581 tccgctaaga cgttgaaaag atactctggc gaaatcgcat accccaaaat atcaacgatt
    14641 gttgcaatca gatcttcttt tgattcttca tctgaaagta attcacgata caacatcgtt
    14701 tgtttgctta ctgtgtcata ttcctctaag gattcatctg ctaaaagaac cacttgttcc
    14761 aacaaacggt cggataaata tttatagaaa atcagcccca ataaataatt tttatattct
    14821 gacgcatcca ttttactacg gagattatct gccgcactaa ataacttctg atttaattct
    14881 gcacccaaaa agatacactc ctatctctta tttaaagttg ctatataccc tcttatttta
    14941 gcatactcag ttttagaaaa agcaaaaaaa taaagaaact tattcttgat aacaagctac
    15001 gtgacgtata tccctaacac aaaaatagcc atatcctttg gtttggatac ggctatttga
    15061 tattactcat tcaattatca aatcacagta ggtgtgaatc tcatcgggat cggcaatatc
    15121 tacttctgat taggatcaaa ggtgattttc tcaataaagg tgtgatacaa agctttaact
    15181 tgcttcttct cactctggat aagaaagcaa tccaaactag tcaatatctg ttggacatct
    15241 ttgacttgaa tcggttcccc ttgttgttga aagatcccta agatttcatt ctctcgaatt
    15301 tgtaactgtc ttcgtttttc agtcaattca tttaaacgat cttttaacat cggaaacaat
    15361 tcgggactat cttccaaagc agcttcctgt ttttgctcta atagttagat tttgcgtacc
    15421 attttcttct tttattttcc ttcgattgct gattttaata tagtctaaag gaaatttata
    15481 tttattgtat tctttataac ttcaagagaa cttcgtttga gtattactgt atttttttaa
    15541 ttccataaga aaattagtta ttacctgcca cctaatcagc aaatctgaac ctgagtcaat
    15601 ataattaata ttattttcca tctccaacat tgtaagtact gttggtgtta atatagggac
    15661 tttgtacaat tgtatttttt tgattttatc atctgcacaa aggagatttt ttaaatctaa
    15721 tccttctttt atttcaaaag ttgaattttt taaatctata agagtaaaac caattccttc
    15781 atactgaatt tctgaaaatc gaatttttct cccagttgat agatcaaggg gcagaaaacc
    15841 ttttattaca ttgtgagtgt caccatcatt tacaatgtca ttaggaattt tgattttaga
    15901 attctgtgga gcacctattg gaagataata attcattata attttgtagt cttcatgagg
    15961 aaataaagta cttttaggat tgctaagata ttctcgtacc gaatctaaga atggcttatc
    16021 cattgaatct tcatccccat ttaaactaaa aaacattcct acaattgctt ttgcaactct
    16081 aagtgaattc attttcttta ttgaatgtgt taaatcagac ttaccattct ttaaacttgt
    16141 attgttataa aggaaatttt tattgtcttc tgcaaatcta atatattcct caacatactc
    16201 actaccaaaa aaactattac atttactaca taacgtattg gaatggcaac agtttttttg
    16261 acaaatttta taaggtgcag aacttctttc cgtatgctat tccgattgtc cttgacaatg
    16321 agcctcctcg gatgtgacga tcttcggcag gagctaacag ttaaagttag actgcctcgc
    16381 tagcgttagc acatccgagc tcattatcaa ggattcgctg cgccaat
//
LOCUS       DACDSR010000055        16355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDSR010000055 DACDSR010000000
VERSION     DACDSR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.055"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWK15_001824"
     CDS             complement(27..725)
                     /locus_tag="IWK15_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9547406.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWK15_001825"
     CDS             complement(718..2070)
                     /locus_tag="IWK15_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9547407.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWK15_001826"
     CDS             complement(2293..3693)
                     /locus_tag="IWK15_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9547408.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWK15_001827"
     CDS             3854..4591
                     /locus_tag="IWK15_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9547409.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWK15_001828"
     CDS             4716..5195
                     /locus_tag="IWK15_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9547410.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWK15_001829"
     CDS             5192..5959
                     /locus_tag="IWK15_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547411.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWK15_001830"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWK15_001830"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9547412.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWK15_001831"
     CDS             complement(6862..7464)
                     /locus_tag="IWK15_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9547413.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWK15_001832"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWK15_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWK15_001833"
     CDS             8145..8729
                     /locus_tag="IWK15_001833"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9547414.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWK15_001834"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWK15_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9547415.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWK15_001835"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWK15_001835"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9547416.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWK15_001836"
     CDS             10630..10809
                     /locus_tag="IWK15_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547417.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWK15_001837"
     CDS             10831..11856
                     /locus_tag="IWK15_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9547418.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWK15_001838"
     CDS             12031..13275
                     /locus_tag="IWK15_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9547419.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWK15_001839"
     CDS             13428..14375
                     /locus_tag="IWK15_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9547420.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWK15_001840"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWK15_001840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9547421.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWK15_001841"
     CDS             complement(15277..15519)
                     /locus_tag="IWK15_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547422.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWK15_001842"
     tRNA            15640..15712
                     /locus_tag="IWK15_001842"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..>16355)
                     /locus_tag="IWK15_001843"
     CDS             complement(15831..>16355)
                     /locus_tag="IWK15_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9547423.1"
                     /translation="SLTITGFIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVIS
                     AAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPV
                     ASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHV
                     IFDFTAILLVVLFS"
BASE COUNT         5573 a         2919 c         3300 g         4563 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaaga
//
LOCUS       DACDSR010000056        16089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDSR010000056 DACDSR010000000
VERSION     DACDSR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.056"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWK15_001844"
     CDS             226..894
                     /locus_tag="IWK15_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9547424.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWK15_001845"
     CDS             complement(1094..1480)
                     /locus_tag="IWK15_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547425.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWK15_001846"
     CDS             complement(1452..2834)
                     /locus_tag="IWK15_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9547426.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWK15_001847"
     CDS             complement(2990..4177)
                     /locus_tag="IWK15_001847"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9547427.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWK15_001848"
     CDS             complement(4184..5215)
                     /locus_tag="IWK15_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9547428.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWK15_001849"
     CDS             complement(5231..6685)
                     /locus_tag="IWK15_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9547429.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWK15_001850"
     CDS             6860..7765
                     /locus_tag="IWK15_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9547430.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWK15_001851"
     CDS             7856..8533
                     /locus_tag="IWK15_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9547431.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWK15_001852"
     CDS             8814..9710
                     /locus_tag="IWK15_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9547432.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWK15_001853"
     CDS             complement(9765..11135)
                     /locus_tag="IWK15_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9547433.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWK15_001854"
     CDS             11373..12119
                     /locus_tag="IWK15_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9547434.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWK15_001855"
     CDS             12116..12955
                     /locus_tag="IWK15_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9547435.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWK15_001856"
     CDS             12956..13906
                     /locus_tag="IWK15_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9547436.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWK15_001857"
     CDS             14047..14799
                     /locus_tag="IWK15_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547437.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWK15_001858"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWK15_001858"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9547438.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5219 a         3009 c         3232 g         4629 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgcttt
//
LOCUS       DACDSR010000057        15954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDSR010000057 DACDSR010000000
VERSION     DACDSR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.057"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(390..812)
                     /locus_tag="IWK15_001859"
     CDS             complement(390..812)
                     /locus_tag="IWK15_001859"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9547439.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(826..2232)
                     /gene="atpD"
                     /locus_tag="IWK15_001860"
     CDS             complement(826..2232)
                     /gene="atpD"
                     /locus_tag="IWK15_001860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9547440.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2255..3157)
                     /locus_tag="IWK15_001861"
     CDS             complement(2255..3157)
                     /locus_tag="IWK15_001861"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9547441.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3171..4727)
                     /locus_tag="IWK15_001862"
     CDS             complement(3171..4727)
                     /locus_tag="IWK15_001862"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9547442.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4750..5292)
                     /locus_tag="IWK15_001863"
     CDS             complement(4750..5292)
                     /locus_tag="IWK15_001863"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9547443.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="IWK15_001864"
     CDS             complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="IWK15_001864"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9547444.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="IWK15_001865"
     CDS             complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="IWK15_001865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9547445.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="IWK15_001866"
     CDS             complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="IWK15_001866"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9547446.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7183..10026)
                     /gene="rnr"
                     /locus_tag="IWK15_001867"
                     /pseudo
     CDS             complement(7183..10026)
                     /gene="rnr"
                     /locus_tag="IWK15_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10029..10775)
                     /locus_tag="IWK15_001868"
     CDS             complement(10029..10775)
                     /locus_tag="IWK15_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9547447.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10887..11123)
                     /gene="secG"
                     /locus_tag="IWK15_001869"
     CDS             complement(10887..11123)
                     /gene="secG"
                     /locus_tag="IWK15_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9547448.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11241..11519)
                     /locus_tag="IWK15_001870"
     CDS             complement(11241..11519)
                     /locus_tag="IWK15_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9547449.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11632..12600)
                     /locus_tag="IWK15_001871"
     CDS             complement(11632..12600)
                     /locus_tag="IWK15_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9547450.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12815..12859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12883..13857
                     /locus_tag="IWK15_001872"
     CDS             12883..13857
                     /locus_tag="IWK15_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9547451.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13862..14437
                     /locus_tag="IWK15_001873"
     CDS             13862..14437
                     /locus_tag="IWK15_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9547452.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14504..15643)
                     /locus_tag="IWK15_001874"
     CDS             complement(14504..15643)
                     /locus_tag="IWK15_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9547453.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4359 a         3443 c         2856 g         5296 t
ORIGIN      
        1 aatctgagcc agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc
       61 cttattgaca aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc
      121 aatcatctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat
      181 ttgtttctct aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc
      241 agaagtagct tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg
      301 taaattcctt cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct
      361 taaacacgga taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg
      421 attgctcggt gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt
      481 gcttcttcga tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata
      541 tcacgttcac gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg
      601 attccgccat taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga
      661 acttcatcaa tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca
      721 ccagctgtcg tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga
      781 gttaccacat ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt
      841 tttcgctttt tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag
      901 atcatcgtat ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac
      961 atatgaacct ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa
     1021 ttggactctg cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc
     1081 taagatagca atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt
     1141 tgctacttta taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc
     1201 caatggatct accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt
     1261 cgcatccaaa tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac
     1321 atagattgct tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa
     1381 ttgacccatt tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa
     1441 caaggctgat acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag
     1501 tacatcttga ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac
     1561 acgcatacgt gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat
     1621 aactcctgaa tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac
     1681 accggtaaat acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat
     1741 cagtacggtt ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata
     1801 tggggcaagc aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa
     1861 ttcgtcaaag gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg
     1921 tgtttctaaa tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc
     1981 tacaggaaca gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc
     2041 tgttgattcc atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag
     2101 aacaactttt gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg
     2161 taaggattgg tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt
     2221 gcctgaactc atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa
     2281 caatttcggt aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca
     2341 gctcatcaat gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag
     2401 catgttctgc tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag
     2461 gcaataactg agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag
     2521 tagttgcttc ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac
     2581 tagttagcga attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt
     2641 ggtacatagt tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag
     2701 atagattgcg cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag
     2761 ttccgccaat tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag
     2821 acatcgtttg tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga
     2881 tgacgatata acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat
     2941 cccctctagg attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga
     3001 tttctcttac ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca
     3061 acttagaagc agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag
     3121 cgatacgtgt cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt
     3181 tgaatggatt tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg
     3241 tctttatatt cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta
     3301 cggattgttt caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg
     3361 aagtctaaaa tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa
     3421 atcactactt gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact
     3481 gtacggcgtc cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt
     3541 gtaaatgctt ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt
     3601 ttcattgctt tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc
     3661 gctggacgag tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc
     3721 gaaataacgt ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt
     3781 aaggcagtca ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag
     3841 cgtgagtgta agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc
     3901 agcaaggaaa gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag
     3961 acatgtttcc cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata
     4021 taaagcaacg gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg
     4081 ccatattttt tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct
     4141 acatagatac agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa
     4201 gttttccctg ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca
     4261 agagcgtcga ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata
     4321 acgcctggtg ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg
     4381 tcaattggct gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact
     4441 tccatgatct ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca
     4501 aggataatga tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt
     4561 gaaaattcaa gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg
     4621 tcacctacat aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca
     4681 atttgttctt taatcaaggc actaatttct tctgctttga tggccattcg attcacctac
     4741 ctctttgtac tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc
     4801 gtcgattact cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat
     4861 ttgttttaat tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat
     4921 tcgttgttcg tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg
     4981 ttcatcatat cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata
     5041 cgcataaaca acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc
     5101 catgatttct cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc
     5161 aggtacttgt tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt
     5221 atttgaatcg atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt
     5281 atctagtttc atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca
     5341 tttctggaga taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa
     5401 catcgtcttt tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt
     5461 tactttttag acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt
     5521 ctccactttc tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt
     5581 gttgttcgcg ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca
     5641 aatcattggc gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa
     5701 aatgcttcaa gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta
     5761 acatcgcatt gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc
     5821 tttgaagtac ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt
     5881 aatactgatt caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag
     5941 gaacagcttc gactagagct accccgataa acattgttgt acggaattga ccagacattt
     6001 caggttggcg agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg
     6061 ctccgattgc tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc
     6121 tcctaaatta gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc
     6181 ttatggctca tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata
     6241 aacaaggaga atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag
     6301 cctaagctta ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc
     6361 ccataaagac gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga
     6421 aacataaaac tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt
     6481 ttcaccccaa ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac
     6541 gggtctgctg tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact
     6601 aaaccgatgt tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa
     6661 ttgctcaatt cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc
     6721 cattctatcg cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa
     6781 gctaagaaga aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac
     6841 caaattggtc caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc
     6901 ccgaaattca cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac
     6961 cacacggtat cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca
     7021 cttttttatt tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta
     7081 agaaaaaacc tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa
     7141 gtaaaagcaa aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt
     7201 taatgttcga tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata
     7261 agattttttc cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac
     7321 ttttaaagga acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg
     7381 ttttttgtgt aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg
     7441 ttcataagga ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa
     7501 tccatctttt agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac
     7561 catccctgct tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc
     7621 aattaatttt ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt
     7681 tttcttcttt ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg
     7741 cttactatcg ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg
     7801 ttttccttcg cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac
     7861 ctcttctgcg gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac
     7921 ttttactcgc actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc
     7981 taaatgattt tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc
     8041 ctctactgta ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc
     8101 atcgaattct tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc
     8161 acgttctgtt tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag
     8221 ttccgtagcc cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag
     8281 acgatggacg attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc
     8341 tgccgctaag ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct
     8401 tagcaacata gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg
     8461 ctgtaaatct tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga
     8521 agcaaaatca aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat
     8581 aaacggcaga tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa
     8641 agattcgatc aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc
     8701 ttcagcatca acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat
     8761 tcgcatcgtt tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg
     8821 agcatagcgt tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta
     8881 ggtcatccgt tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc
     8941 atttggatca atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga
     9001 acagatacca ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac
     9061 acttgtccct ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga
     9121 aacatctgca atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac
     9181 tgcatcatct agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa
     9241 atctctacgt ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga
     9301 caatacatca tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc
     9361 catgccaggg tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc
     9421 ataccctttt tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg
     9481 aactccctct gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac
     9541 aacgccgtaa agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc
     9601 taactgagta gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat
     9661 atctgtattt gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc
     9721 agtcgcttct tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa
     9781 tcctcgttca tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac
     9841 ttttcctttt ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac
     9901 tagtaattta aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga
     9961 aaaactgcgt ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt
    10021 tgtcatattt attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct
    10081 aattcgcgtc ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt
    10141 gtcactcgct gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt
    10201 tcatctttcc cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct
    10261 acgattgctg cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca
    10321 actgcttttt cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg
    10381 tatttttcaa agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa
    10441 ccgccgccaa tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat
    10501 aatcctaata cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta
    10561 gtgtctgccc accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg
    10621 tgtcctttaa aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc
    10681 attcgaacat cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca
    10741 tgttttgcat aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt
    10801 taccatagtt tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc
    10861 cgccgttttg cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat
    10921 aagatcatcc agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg
    10981 cgagcttttt gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta
    11041 ttttgcttgc ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg
    11101 attcccaaaa ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa
    11161 ctttatcata gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc
    11221 aaaactttca gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt
    11281 tcaaactctt tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct
    11341 aattctactc cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct
    11401 acaaatgtat ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg
    11461 atctcttgtt gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata
    11521 tgccgcactc ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg
    11581 atctataaac aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat
    11641 cgttattctt tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat
    11701 cgatgcttcc aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc
    11761 attttgaatc gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac
    11821 caataaaggc agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta
    11881 accggcgtat ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac
    11941 agtgatttta cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa
    12001 aataaatcct ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc
    12061 tcccattgat ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg
    12121 gatccaggca attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact
    12181 ttctccgtgc gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt
    12241 acctatatga gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca
    12301 tttattctgc ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact
    12361 tttgattgtt ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg
    12421 tcgttttttt tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc
    12481 ataccagtca tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga
    12541 aagaaaatgg taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat
    12601 tgtacacccg ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat
    12661 aggattcttt attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta
    12721 aagttctcct caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta
    12781 tactaatttc gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag
    12841 aataaaaaac taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt
    12901 gattgcgatc cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat
    12961 atcgaagtag ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag
    13021 aatgtgctga aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg
    13081 gaaaaacctt tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta
    13141 ggggaaacaa atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat
    13201 tttttggctg attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg
    13261 accaatatcg cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt
    13321 gtctcgatgg gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat
    13381 tactggtgtg atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa
    13441 atggttgggt tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc
    13501 tgtcaaatga atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac
    13561 ttccactgga aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct
    13621 tattttatcg agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa
    13681 ggaataagtc gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac
    13741 agtaaaatta tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt
    13801 ttaaatgcac aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa
    13861 aatgaataaa aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat
    13921 caattatatt ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat
    13981 cggaacgatt tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc
    14041 cagcggtatt ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca
    14101 aattctcaca ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa
    14161 cagcttgaaa ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc
    14221 gatcattacc gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca
    14281 gctattacat gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac
    14341 agactacctt gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc
    14401 acaacgcttg atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa
    14461 ataatgctaa tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata
    14521 aaataattcc ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat
    14581 ctacttgatg aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt
    14641 tagctttcgt aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct
    14701 ggctgaattg ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta
    14761 ttttgttgta atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga
    14821 cgattgattg cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt
    14881 tcccttggag ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg
    14941 taacattatg gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca
    15001 tttcagcatt tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt
    15061 ctggcataga gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca
    15121 tgtgcgtata catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat
    15181 ctacatagcc tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag
    15241 tggctaataa tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg
    15301 caatcaccat tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt
    15361 tcccatggat ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca
    15421 cgacatccat atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag
    15481 caatcaaatt atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg
    15541 ccaaaagctt attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc
    15601 ggatgatctc ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc
    15661 catttctttt tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa
    15721 aatgaagaaa gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta
    15781 tacgagagga cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat
    15841 gatgctagtt attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg
    15901 taaacatcat atacagaagc tagttgcaaa aaaaactctc tacctgctga tatt
//
LOCUS       DACDSR010000058        15741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDSR010000058 DACDSR010000000
VERSION     DACDSR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.058"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWK15_001875"
     CDS             138..1475
                     /locus_tag="IWK15_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9547454.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWK15_001876"
     CDS             1523..3652
                     /locus_tag="IWK15_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9547455.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWK15_001877"
     CDS             3627..4316
                     /locus_tag="IWK15_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547456.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWK15_001878"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWK15_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWK15_001879"
     CDS             complement(5546..5740)
                     /locus_tag="IWK15_001879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547457.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWK15_001880"
     CDS             complement(5790..6230)
                     /locus_tag="IWK15_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9547458.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWK15_001881"
     CDS             complement(6234..7079)
                     /locus_tag="IWK15_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9547459.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWK15_001882"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWK15_001882"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9547460.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWK15_001883"
     CDS             complement(8703..9650)
                     /locus_tag="IWK15_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9547461.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWK15_001884"
     CDS             complement(9873..10223)
                     /locus_tag="IWK15_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9547462.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWK15_001885"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWK15_001885"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9547463.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWK15_001886"
     CDS             11646..11966
                     /locus_tag="IWK15_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9547464.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWK15_001887"
     CDS             11988..12452
                     /locus_tag="IWK15_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9547465.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWK15_001888"
     CDS             12657..13262
                     /locus_tag="IWK15_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9547466.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWK15_001889"
     CDS             complement(13301..14590)
                     /locus_tag="IWK15_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9547467.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWK15_001890"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWK15_001890"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9547468.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4947 a         2915 c         3222 g         4657 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga t
//
LOCUS       DACDSR010000059        15634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDSR010000059 DACDSR010000000
VERSION     DACDSR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.059"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(204..570)
                     /gene="ssrA"
                     /locus_tag="IWK15_001891"
     tmRNA           complement(204..570)
                     /gene="ssrA"
                     /locus_tag="IWK15_001891"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(741..1514)
                     /locus_tag="IWK15_001892"
     CDS             complement(741..1514)
                     /locus_tag="IWK15_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9547469.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1658..2008
                     /locus_tag="IWK15_001893"
     CDS             1658..2008
                     /locus_tag="IWK15_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9547470.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1989..2705
                     /locus_tag="IWK15_001894"
     CDS             1989..2705
                     /locus_tag="IWK15_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547471.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2705..4153
                     /locus_tag="IWK15_001895"
     CDS             2705..4153
                     /locus_tag="IWK15_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9547472.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4223..4732)
                     /locus_tag="IWK15_001896"
     CDS             complement(4223..4732)
                     /locus_tag="IWK15_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547473.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4722..5447)
                     /locus_tag="IWK15_001897"
     CDS             complement(4722..5447)
                     /locus_tag="IWK15_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547474.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5670..7790)
                     /locus_tag="IWK15_001898"
     CDS             complement(5670..7790)
                     /locus_tag="IWK15_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9547475.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8020..9198
                     /locus_tag="IWK15_001899"
     CDS             8020..9198
                     /locus_tag="IWK15_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9547476.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9214..9972
                     /gene="aroD"
                     /locus_tag="IWK15_001900"
     CDS             9214..9972
                     /gene="aroD"
                     /locus_tag="IWK15_001900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9547477.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9995..10480
                     /gene="ybaK"
                     /locus_tag="IWK15_001901"
     CDS             9995..10480
                     /gene="ybaK"
                     /locus_tag="IWK15_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9547478.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10551..11804)
                     /locus_tag="IWK15_001902"
     CDS             complement(10551..11804)
                     /locus_tag="IWK15_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9547479.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11922..13271
                     /gene="gdhA"
                     /locus_tag="IWK15_001903"
     CDS             11922..13271
                     /gene="gdhA"
                     /locus_tag="IWK15_001903"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9547480.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13383..14729
                     /locus_tag="IWK15_001904"
     CDS             13383..14729
                     /locus_tag="IWK15_001904"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9547481.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15037..15423)
                     /locus_tag="IWK15_001905"
     CDS             complement(15037..15423)
                     /locus_tag="IWK15_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9547482.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15472..>15634)
                     /locus_tag="IWK15_001906"
     CDS             complement(15472..>15634)
                     /locus_tag="IWK15_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9547483.1"
                     /translation="VFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFE
                     GTLRYSLGY"
BASE COUNT         5105 a         2796 c         3150 g         4583 t
ORIGIN      
        1 tacgaacaaa agttcttttt gtaaacctta aaatagattt tcctgaatac gtattgggga
       61 tttctaaatg tttgaatgtt tttttgaatg caaaatcaat gcaaaaaatt ttgtatttct
      121 ctacttttct ctccttgacc aaaataaaaa aacgttgatt taacaacgtt tttcatacat
      181 gtttatctgt ctcaaccata catatatgga gacggcggga gtcgaacccg cgtccaaaca
      241 catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac tttacagctt
      301 gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac ttacagacgg
      361 aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg gcgatgccga
      421 gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt cgtttttagc
      481 agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca attcaagctc
      541 gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg tgtatttttc
      601 atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa tcatatccca
      661 ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct taagaaaaga
      721 ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg gacaattccg
      781 ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc ataggcgttg
      841 cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg accatcgcca
      901 aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt gacacctgcc
      961 ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg ataaaaagtt
     1021 agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat cagcaacata
     1081 tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga atgaatgaag
     1141 tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg atcgttatag
     1201 taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca ttcttctgaa
     1261 gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc attcatggtg
     1321 accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc tgtacgtcct
     1381 gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt tgccacttcg
     1441 ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa agtgattgca
     1501 cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct taacaaagaa
     1561 tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag tcaacaattt
     1621 agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca gtattggtat
     1681 cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa atctgccttt
     1741 ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc tggctcaaaa
     1801 gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg gctattattt
     1861 aaagacatct gggcatacga attattttct gattgaaatg cttctgctag tatttccaat
     1921 tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg aaactggagg
     1981 gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa atgatgcaga
     2041 tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa gaagggctcg
     2101 atgccttttt agaaaaccca caacattggg atatgattac actagacctg aatttgccca
     2161 cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact gtgccgatta
     2221 tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag atgggggcag
     2281 atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg aaagctttgc
     2341 atcgccgtag tgagttggca gaagcaagag aagaagcagc agtttcagat gatcattttg
     2401 atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat ctggacaacc
     2461 ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta gcaaaaaaac
     2521 cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat cagtactttg
     2581 gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc gaaaaggtcg
     2641 gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat tctggtgttg
     2701 cctaatgcgt tatttgtatc agcaactctt agctttttgc ggaatggtcg cgttgatctt
     2761 attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag aaaacaatta
     2821 ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct atgccgatac
     2881 gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga cagagcaagt
     2941 actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg tcatttatcc
     3001 gactaataca gctttactga attttacctt aacagctgat caatggaaaa atctgaggaa
     3061 tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac aggcgacctc
     3121 ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg tagttagtca
     3181 acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt tcaaaggatt
     3241 tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat tccaagttaa
     3301 gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt ttgatgtaca
     3361 gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca ataaaatgac
     3421 gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc gaagacaatt
     3481 tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg gacttctgga
     3541 agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta aattgatgaa
     3601 aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg aaaaaatccg
     3661 tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat tgaaaaatat
     3721 tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat tgaaagccgt
     3781 tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg tcaatattat
     3841 tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag aaaaaggcta
     3901 tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac aacaaatgaa
     3961 aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa aatatggtga
     4021 atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag gaaaattaga
     4081 agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg acgtagaaat
     4141 caaaaaagag taaaaaacaa gcggtataaa agatcatgaa tcgatcttct ataccgcttg
     4201 tttttactat aaaatgattg atttagcccg cgggctctaa acgatcaata taaggacttt
     4261 gagcaatagt agcatcatga gtaaagagta agataccttg attttcttca tgagctaaat
     4321 aagaaagcaa gtggagtagc tcttggatct ccaatacact aagttcatca aatgtatctt
     4381 caataacaat attgtttcgc ttggaaagca aggcatgaag taattgcagc tttacttttt
     4441 caaaagacgt caactcagct gccgcacaat taagcaagga aggagagata tcaagttttt
     4501 cttgataggc caaacgatct tgtttgattt ctttttccgg tacatccaac aggacatggt
     4561 ctttaagatt cagataagga aacatcggcc aatttttttg gatgatcgca aaattatcca
     4621 gttttgcaac aagaacctct tttttcaagt gctccaatat tttatgctgt ttttcgagag
     4681 gacacaaaat aacggtcaag tgttcagcat gaatcaaata ttcaggtttc attacgtcca
     4741 ggcctcctac tttgcaaatg atggataaaa aaagtgaaag ggaatagact aagaatgaga
     4801 cagcatccac tgagcccaat aagagtttgg gcaaatccct taagtatatc tgtgaaaaaa
     4861 agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat ccatcggaat tgtaagtccg
     4921 cttaccgaag tcaagatatc acggatatct ggcagttcag tagtctttaa gacaagaaaa
     4981 ttgatccttt gaagaagcgc ttcataaact gactggaagc ttacgatcca caataaaacc
     5041 aagatacaac aaagaaagag aggaaagaac agctgccaga aaataaaaag gaataatttg
     5101 gttttagaaa gaccagtcag gcgccaattg atcaattcct gcttgttttg cctgactgaa
     5161 tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa ataaactgaa tatgcctttt
     5221 atataaaatc ggctagctaa ttctaaagcc ggaagaagct ctgtagatgc agaagcgcca
     5281 ataaatgaaa gccgatcttg gacttgattg gctaaagccg attgcaaatg gtaaagagtc
     5341 aggcatgcta tcaataaaaa agcagaaaag cttgaaaata aacaatagag tattgtctgc
     5401 tttttgtaat aaattaatcg agcagtagca tagcggatta ttttcatact tttcacctct
     5461 ataaaaagta tagaaaatga atatgaataa agtatgaaat tcctttagtg aaatcacgaa
     5521 ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg aagaagctgc cctattctat
     5581 taccgtagat gcaagctttt gaacactgtt tattcgaaaa gaggagcagc gacaataatt
     5641 ttgtcgctgc tccttttaaa tttattttgt cattcggaag aacttgtggt cgttccgtga
     5701 agttcttggt acgtttgcgt tttataaaga tcaacagttg attgtccgtt gtgttcacta
     5761 aagacactag ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca
     5821 ttcttgtagt cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga
     5881 agaagatcac cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc
     5941 agtgaatcaa tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg
     6001 ttactataga tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg
     6061 aaagaaacaa gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt
     6121 tttgtatcta cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa
     6181 gtgtgattga ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc
     6241 atttgtgcat tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga
     6301 tttttatttc ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta
     6361 tcatacaagc cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa
     6421 ttggctgtcg caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct
     6481 gcttcgtcat tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa
     6541 taaaatggtt gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca
     6601 tgcaacccgt attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa
     6661 taatcatatc ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca
     6721 tggaataccg cactagtgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa
     6781 gtattttttc cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt
     6841 tccatcagcg tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca
     6901 aaactgaacg tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg
     6961 agaccattct catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg
     7021 atataaatca cattttttcc tttagcgagc ccgaacaaat cgtcatttgg tgctgcataa
     7081 tgttctttta cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact
     7141 tggtttgtct ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat
     7201 ttgaccaaat aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc
     7261 ataaaaagat ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt
     7321 gctcggatcg ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata
     7381 ataaaatcga tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc
     7441 agaccactcg ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat
     7501 tctcgataat aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc
     7561 atcgtgatat aagcaactct agggcgtctg atataaagag caattccaag taataacaat
     7621 gtagttgcaa tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga
     7681 tggaaatcaa ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa
     7741 aaaaatccga gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc
     7801 ctttctgcac ttcttaatca cagaagtgtc atgaaaccat gttacacagc tcacttattc
     7861 tcatttattt tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt
     7921 cattaaggtt ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt
     7981 tgttatactt gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa
     8041 agagcaagca atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat
     8101 ccccaaaaag aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca
     8161 aaaggatatc taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc
     8221 ccatttgatc aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc
     8281 tttttttcag atgattctac taccgctttt cgtatattca atggagaagg agacggaatc
     8341 ggcggtctga ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg
     8401 ttatatgcac atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcgta
     8461 ggaggctatg agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac
     8521 ggaaaagagg caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat
     8581 ttaaatgaag gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta
     8641 gcagaaggtt tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt
     8701 tctgttgctg ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc
     8761 agtttaaaaa aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa
     8821 atcattgtta tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat
     8881 gacatgattg tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta
     8941 gcaaaagatt atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata
     9001 cttattgcgt ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa
     9061 gaggaattcc atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa
     9121 gatttccaga cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa
     9181 attaaaaata accattagaa ggagcgatgc agaatggttt gggtaaaggg attgaatata
     9241 ggaagcggta gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta
     9301 cgtgaagcag ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat
     9361 tatcgtgaag ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg
     9421 cttgatggac tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac
     9481 atatcgatcc gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat
     9541 atggtcgata tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc
     9601 aagtctttaa acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac
     9661 cctgtcttgc tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg
     9721 gcagtaactc ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca
     9781 aatgcttttg tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct
     9841 cgtctggctg gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct
     9901 acttctgctc caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat
     9961 gaaccgttgt agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac
    10021 gaatgcaatc cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg
    10081 ggacgaaagc cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctcg
    10141 tatatttaaa acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc
    10201 gggagaagcc gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga
    10261 aatgctccat ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc
    10321 tataggtatg aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca
    10381 aatcattgtc tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg
    10441 tgcattaacg agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag
    10501 aaggaggccg ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt
    10561 tgcccggcat tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg
    10621 ccagtataac cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta
    10681 ctctccaatg ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat
    10741 ttctcaatag gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa
    10801 tcttgaattt gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca
    10861 agaaagaaaa ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt
    10921 ttggttgctt tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg
    10981 aaacaatcca gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa
    11041 cttgaacaac gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc
    11101 gtatgttcag tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa
    11161 gaaagccctt gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct
    11221 gtcgcataaa gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca
    11281 gtagaaccag tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg
    11341 aactcttcga attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct
    11401 actcgagtag agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg
    11461 ataatcgttt ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa
    11521 tgtacgcctt gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta
    11581 ctatataaaa actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg
    11641 ttcgtaacat tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt
    11701 ttttcgatca agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg
    11761 cttgttccag cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga
    11821 aagtgtagca tatctgaaga aagaaaacag aaaaaaaatg ttttgagcca aaagagaaaa
    11881 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
    11941 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
    12001 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
    12061 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
    12121 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
    12181 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
    12241 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
    12301 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
    12361 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
    12421 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
    12481 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
    12541 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
    12601 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
    12661 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
    12721 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
    12781 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
    12841 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
    12901 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
    12961 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
    13021 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
    13081 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
    13141 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
    13201 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
    13261 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
    13321 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
    13381 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
    13441 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
    13501 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
    13561 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
    13621 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
    13681 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
    13741 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
    13801 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
    13861 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
    13921 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
    13981 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
    14041 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
    14101 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
    14161 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
    14221 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
    14281 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
    14341 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
    14401 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
    14461 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
    14521 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
    14581 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
    14641 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
    14701 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
    14761 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
    14821 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
    14881 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
    14941 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
    15001 ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta atttttcttt
    15061 tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc gcttttcgct
    15121 tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag agtcaccaga
    15181 agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag catctacgtt
    15241 gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag tttctgcagg
    15301 cattggagct ttgactaccg cataataatc tttacctaca taagtagact gatagtagtc
    15361 ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta aaattttttt
    15421 catgaaagat ctcctttttt atttttatta tacccgtggt gctagttaat tttaatatcc
    15481 taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata gaacactgta
    15541 agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg agttgaaatt
    15601 ttgacaacct aatttttcta aggaagaaaa aaca
//
LOCUS       DACDSR010000060        15404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDSR010000060 DACDSR010000000
VERSION     DACDSR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.060"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            157..2217
                     /locus_tag="IWK15_001907"
     CDS             157..2217
                     /locus_tag="IWK15_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547484.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2300..2869
                     /locus_tag="IWK15_001908"
     CDS             2300..2869
                     /locus_tag="IWK15_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547485.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3044..3850)
                     /locus_tag="IWK15_001909"
     CDS             complement(3044..3850)
                     /locus_tag="IWK15_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547486.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3988..5502
                     /locus_tag="IWK15_001910"
     CDS             3988..5502
                     /locus_tag="IWK15_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9547487.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5905..6888)
                     /locus_tag="IWK15_001911"
     CDS             complement(5905..6888)
                     /locus_tag="IWK15_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9547488.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6893..7540)
                     /locus_tag="IWK15_001912"
     CDS             complement(6893..7540)
                     /locus_tag="IWK15_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9547489.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7625..8257)
                     /locus_tag="IWK15_001913"
     CDS             complement(7625..8257)
                     /locus_tag="IWK15_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9547490.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8273..8824)
                     /gene="coaC"
                     /locus_tag="IWK15_001914"
     CDS             complement(8273..8824)
                     /gene="coaC"
                     /locus_tag="IWK15_001914"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9547491.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8821..9558)
                     /locus_tag="IWK15_001915"
     CDS             complement(8821..9558)
                     /locus_tag="IWK15_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9547492.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9555..10544)
                     /locus_tag="IWK15_001916"
     CDS             complement(9555..10544)
                     /locus_tag="IWK15_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9547493.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10802..11344
                     /locus_tag="IWK15_001917"
     CDS             10802..11344
                     /locus_tag="IWK15_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547494.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11361..12446
                     /locus_tag="IWK15_001918"
     CDS             11361..12446
                     /locus_tag="IWK15_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9547495.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12458..13264
                     /locus_tag="IWK15_001919"
     CDS             12458..13264
                     /locus_tag="IWK15_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547496.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13265..14092
                     /locus_tag="IWK15_001920"
     CDS             13265..14092
                     /locus_tag="IWK15_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547497.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14089..15162
                     /locus_tag="IWK15_001921"
     CDS             14089..15162
                     /locus_tag="IWK15_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9547498.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4950 a         2781 c         3138 g         4535 t
ORIGIN      
        1 acataagtta ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta
       61 atagtccatc ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact
      121 tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa
      181 caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac
      241 gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta
      301 cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact
      361 gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct
      421 gcattgactt cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt
      481 cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc
      541 gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat
      601 gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat
      661 tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct
      721 gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt
      781 tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc
      841 tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct
      901 gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct
      961 aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc
     1021 aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat
     1081 atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct
     1141 gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa
     1201 gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt
     1261 tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct
     1321 atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt
     1381 gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga aattaccttt
     1441 acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca
     1501 aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc
     1561 gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg
     1621 accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa
     1681 gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa
     1741 gaacaaaaaa gacgagaaga aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt
     1801 tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa
     1861 atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga
     1921 ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct
     1981 aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg
     2041 tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga
     2101 acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg
     2161 gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt
     2221 atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga
     2281 gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac
     2341 aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt
     2401 ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca
     2461 aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact
     2521 tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc
     2581 ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaacc
     2641 agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc
     2701 taccctccat acggacggac caatctatct tcacacacag acatggagcc atcaggcgat
     2761 tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa
     2821 tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga
     2881 aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt
     2941 gagttaatac tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga
     3001 caaaagtatt gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt
     3061 taggaataaa attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac
     3121 caaatacact gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca
     3181 tgattcccac gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat
     3241 tttgactttc aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt
     3301 tacttaactc cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca
     3361 gtaatttatc tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac
     3421 agaaggaaac aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga
     3481 gcaaataaac aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa
     3541 aagtatccca cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac
     3601 taaaaataaa agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct
     3661 tttccagaaa ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc
     3721 cccagtgttg gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa
     3781 tatagattcc gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca
     3841 aatgattcat aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt
     3901 accaaagcta tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata
     3961 tagtataagg agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc
     4021 gttgcacttc ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt
     4081 tctagtacga aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat
     4141 ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg
     4201 gcagcagcta tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct
     4261 gttgccggag gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt
     4321 caaaaagaag aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt
     4381 ggtggaggaa cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg
     4441 gtcgattggc tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg
     4501 agtgaaaaga ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag
     4561 gggttattga ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca
     4621 gacattaaga aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa
     4681 ccaaaagagg tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa
     4741 gaatttattg aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga
     4801 tcaactggag atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat
     4861 aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt
     4921 cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat
     4981 acaagggata aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt
     5041 tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat
     5101 gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca
     5161 ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc
     5221 aaccgtgcca cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc
     5281 gcaccaggaa tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta
     5341 gataaagaaa accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta
     5401 catggaaaca accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa
     5461 gcgggcatac aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa
     5521 agaggttgtg aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt
     5581 agtgaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac
     5641 agcttcttat gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc
     5701 gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     5761 cacgtataaa cggcggaagc agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa
     5821 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
     5881 gcctcttttt tgatcagcat gattttattc agttatcttc aaaatgattt ttccgatgtt
     5941 tttgtttgct tccatatatt catgcgcttg tttgactgcg ctgaattgga atgtacggtc
     6001 aatgattggc cgaatcttgc cattttctag aaaaggccca gctttttgca taaaagtttg
     6061 tgcgagctct gctttatact gatcacttct aggtgtcaat aatgtaccaa gcagttggac
     6121 acgtttccca atcaatacac caaggtctat ctcaggcacg atggtcccac ctagcatacc
     6181 aatcaagacc caacgtccat caacggctat agaagctaaa ttcttttccc agtaagaagc
     6241 gccgatgaaa tccaaaataa catcgactcc ccgaccttca gtgatttttc gtacctcttc
     6301 ttgaaagtct tgttttttat agttgatcaa ttcatctgca ccaagttctt tacaaaaggc
     6361 gagtttttct tctgatcctg cagtaacgat gattttcgcg tgagaaagct gtctagctaa
     6421 ttggatcgct gctgttccca caccacttgc tcctgcatgg atcaagacag tttcgccttc
     6481 ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc
     6541 agcagcagaa acatagtcta gcgtatcagg gagaaggatt gcacgatcgg caggcatcac
     6601 agcatattcg gcataaccac ctagattgac taatccgtat acttttgtcc caggagtaaa
     6661 agtggagata tcacttttat tctcaactac ctctccagct acttcgactc ccaaaatagg
     6721 gtagggaggt ttcaaagaga ctgattctct tttcataata tctgttcgat tgacagctgc
     6781 tgtatggact ttgatcagta attcgccttg ttttggttta ggaacaggca cttcaatttg
     6841 ttctaataca tctacagaac caggatgctt gattgaaatg gcacgcatgg atctattcct
     6901 cctttgccca ttcttggaat gctgtattca actctttgct accttcagaa agtactagag
     6961 tcgtacgaga aatatccgtt tcttccacta gagcggcaag tacagcagca actgtagagc
     7021 gagtgatcgt ttttgatgcc ttctcattag gcttagttaa ttgaatggaa gtaacttctt
     7081 catcgttagt caatgtgact ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc
     7141 attcatctgc ataatgttta gtaatataat aatctgtcat ggactcaggc cattttgctg
     7201 gttcatctgc ataaacagca ctgaccatca gatagcgaga gacatgcgca ttttcagctg
     7261 cgatcactgt tttgattgcg ccatccaagt ctacttgcag aagattcttt ccttgagagc
     7321 cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc ggtcattttt tccacggacc
     7381 aagttaaatc aaaagagaca tacgaaacat tctctttttt tactaatgtt tgggaagcaa
     7441 catctctcac ccctgcaaaa atttcatggg aacttggagc taaatctttt atcaggtgtc
     7501 ggccaatttg tccgtttgca cctacaacaa aaattttcat ttggatcatc ctttcgacgt
     7561 caaatctttc cattcaatag taacaccaaa agagaaaaaa atcagagatc ggctacgatg
     7621 actatcagtt agtaagagaa gggtgcttga agtatcttaa caagacattg catactgctg
     7681 cagtcgcaag acctgcgacg attccttcag gtataccact tgtaaaaata accgctagaa
     7741 tagctgaata aacagcattt aaatttgttc cgatgacttt ggcataatct tgctggaata
     7801 aaaagtagat catattcatc actaaaatcg tattggtcaa tgaaccggaa agtccagcta
     7861 agaataaaga aacggaatct gctttttgtt tcattagttt gcgcagacct ttataaacga
     7921 aataaggaac caccccaatc aagatgcgag gaataaacgc gatcagcaag gctttccagc
     7981 ttccatgttc tgttccgatg actgggataa acggagaaaa gacaaaagac aaaggtgttt
     8041 ggataactgt gctacggatc atgctgatca gtccaaatgt accgccaaga aaagcaccta
     8101 gtcttggtcc taaaacgatc gaagcaatga ttacaggaat atgcatggtt gttgcattga
     8161 ttggaccgat agggataaaa cctaaaggcg taacagctaa taaaatcaaa atagctaaaa
     8221 acatcgcagt taatgtaaag ttttttgtat ttttcataag tactcctttt ttctaagcat
     8281 ttctttcatt aagagtcagc atgacctgtt cgatgatcgt ctgaatatca gctaaggcac
     8341 cttttccaaa atctccacaa gctaaaagag attctcttgg ttcaatttcc tgatagccga
     8401 cagatctcaa tgtttccaga ttacgctgag tgatcgggtt ttgatacata ttcgtgttca
     8461 tggcaggggc aatcaatttt ggtgttccca ctggaagcgc cattgcgact gttgaaagaa
     8521 gatcgtctgc aatgccgttt gctagcttgc cgataatgtt tgctgtagca ggcgcgatga
     8581 gaaacaagtc agcttgtttt gctagttcga tatgattgat tacctgcgga tttgattctt
     8641 gcatgacgtc tgtatggact gcgcgcttcg acagagattg caaggttaat ggcgtgataa
     8701 aatgtgtgct gcttttagac ataatcacat ctacctgggc acctaatttg acgaattgac
     8761 ttgtaagatc agcgcttttg taagcagaaa tacttccagt aacgcctagt aagatctttt
     8821 tcattttgat tcctccagtc taattttctc agtaatcaat gcagcaatct cagatttact
     8881 ttgagcttct tccactgttc catctttaga taagagatag ccgtggtgtg tctcgccatg
     8941 gatttccgtc aaatcatttg cgaaaataaa atcagttttg ttatttatca gactattttt
     9001 tgcgactgcc agaagagatt cctttggtac atctactaat agtttgaaac caatgatttt
     9061 tgcttttggc tgccattcac gtagataaga aatgattttt ggtgttttct tcaaaacgag
     9121 cacaagatgc tcggtgttag aagagatctt tgaaacagtg gtatctgtct gttcattttc
     9181 cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg
     9241 ttcttctgaa aaactaaaag caggagtaaa atcactgact gccatactgt ggataatcgc
     9301 atcgtagtgt tcttttttcg ttagttcttc taattgtaaa aataagtcca gtgcagattc
     9361 gatcgtataa atcgttatat tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac
     9421 gtaatcgatt gtggtatccg gattggcagc taaataatca gctaatgctt gccctaatcg
     9481 tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc
     9541 tgtgattaga atcttcattt atggtcacct acatttacta gtgttcttcc ttggtgtgtt
     9601 ccagcaagta gtttagatgc tgtttcaggc aattgatcca gagtaatctc ttggacgacc
     9661 agttcatctg caatctgcaa atccgccatc tgttgccata aagaaagacg ttgatctatt
     9721 ggtacattga cagaatcaat tccaatcaac tggatgtttc gcagaataaa aggcaagaca
     9781 gtcgcgttta gtttgattcc cccagcattg ccacataaag ctactgcacc gttataagag
     9841 atcaatggaa gaagactgct tagctgctcg ccacctactg tgtcgatgac atagtcgatt
     9901 tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg tacgacttgc
     9961 aaagcaccta gactttttag ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt
    10021 gactggaatc ctagtttatg taacagggct atcgctgtac tgcctacacc gccagaagca
    10081 ccagtaacga caactttttt atcggaggtc ataccttgac ggattaaagc gttcacacac
    10141 aacatagcag taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt catatttttt
    10201 ggtagaggaa ctaaccagtc tcctggtact ttttgatatt ggctataacc gcctggatgg
    10261 gtgacgccaa gcccaaaacc agtagcaagt acaagatctt cttttgaaaa acgtggattg
    10321 ttactttgga cgacagttcc ggataaatca atccctggag tcatcggata tgttcgtatc
    10381 acctgaccag actcgctcat tgccaatgca tctttataat tgacatcaga ataggcaatt
    10441 tttaccaaga cttcattttc agccaactca gtaaatggca gatcactgat tgctgtatgg
    10501 aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt
    10561 ttaaaatttt cttttatcat accataaagc ctactctttt attcttgttc ttccgtaaat
    10621 ttagacaaaa attaggtgaa aaataaagaa tcaaacaact agaatttatt ccaaaagata
    10681 aagaaatatt cagcaaaaaa aattttacaa agggttgcct ttttcaataa atttcgtata
    10741 ctaatacgag tggtagtaaa caggaagttt actattagaa aacttcgatt cggaggaaat
    10801 tatggaaatt ggtgagaagt taagaaattt gcgcatccaa aagaatttga cacaagaaga
    10861 actaggcgaa cgcacagatt tgtcaaaagg gtatatttcc cagttggaac gagatttaag
    10921 ttcaccatca atggaaacat tcttttctat tcttgaagtc ttaggtgtga ctccagaaga
    10981 attttttcat caagaagcag cgaatctgca agtggtttat tcaaaagaag atcatacggt
    11041 ttatttcgat gaagaaaacg ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa
    11101 agaaatggag cctgtcttgc tgacttttga aggtgcagga gaatataaga cgtttgaacc
    11161 ttcattatct gaaacgttta tttttgtatt agaaggggaa ttagagttaa gcttaggaga
    11221 gacggtctat gtggcaaaag caggacagtc gatctattat caagcgacaa aacaacatca
    11281 attaaggaat catctcaaaa aacgaacaaa agtattgatt gttgcaacgc aatcatattt
    11341 gtaatatagg gggtaaaaac gtggggaaac caatcatttc atttgatcaa gtagtgaaac
    11401 gctatgatga tgaaacagta ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt
    11461 atacactgct tggtccttcc ggctgtggta aaacaacgat cctacgtatt atagctggat
    11521 tcaccgaagc tacagaaggg gacatctatt ttgaagggaa acggttgaat gatttgccag
    11581 ctaataagcg acaagtaaat acagtcttcc aagattatgc actttttcct catatgaatg
    11641 tttttgataa cgttgctttt ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa
    11701 agaaggtaaa agatgctttg cgtatggtcc agcttcctgg atacgaaaaa cgggagatta
    11761 gtgaaatgtc tggtggtcaa agacagcgtg tagcgattgc acgagcaatt gtaaatgaac
    11821 cgaaagtatt gctattagat gaaccgttat cggcgcttga tttgaaacta cgtacagata
    11881 tgcagtatga actgcgggat ctgcaacaac gattaggaat cacgtttatt tttgttactc
    11941 atgaccaaga agaagcgttg gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa
    12001 tcgtccaaag tggtacaccc gtagatattt atgatgagcc aatcaatcat ttcgttgctg
    12061 attttgttgg ggagagcaat atcgtggacg gtgtaatgat cgaagataat ctagttgagt
    12121 ttgttggtaa gcaatttgaa tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag
    12181 ttgttttacg cccagaggat ttgacgatca cgacaccaga taaaggaaaa ctagtcgtaa
    12241 cggtcgatac tcagctgttt cgtggtgtcc attatgaaat catctgttat gatgaacagc
    12301 aaaatgaatg gatggtccat tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg
    12361 cctttgagcc agaagatatc catgtcatgc gtttcaacga gtctgaagaa gaattcgatg
    12421 cacgtcttga cagctacgaa gaataggggg gacaagaatg aaaacaatgc gcaggatcta
    12481 ttccgtccct tatattttgt ggctcggttt attcgtgatc gcgcctgtat taatgatcat
    12541 ttatcaatct ttttttgata tgaatgggca attcacgctg aataattatc tatcttattt
    12601 tacgtcgggg acatcgcttt ctatgacgat caattctgta tggtatgcat ttttgatcac
    12661 gtttttcacg ctgatcatca gttacccgac tgcttatttt ttaacaaaac tgaagcacaa
    12721 gcaattatgg ctgatgctga ttattttgcc tacatgggtg aatctgctgc ttaaagctta
    12781 cgcatttatt gggattttca gtgtgcacgg cagcgtgaat catttctttg attttatcgg
    12841 gatcgggccg aaacagatat tattcacaga cttcagtttt ctgttcgtgg caacgtatat
    12901 cgagattcca tttatgatta tgccaatttt caatgcattg gaagagatga atccttcttt
    12961 gatcagtgct agtagagatt taggcgcgaa tagctttgag accttccgac gagtgatttt
    13021 tccgttatca ttaaatggtg tgaaaagtgg tgtccaagca gtatttattc cttctctttc
    13081 tttattcatg ctgactcgtc tgattggcgg aaatcgggtg atcactttag gaacggctat
    13141 cgaacagcat ttcttggtta cacaaaactg ggggatgggt tctacaatcg gtgtgatttt
    13201 aatcattgct atgttcatcg tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc
    13261 ataaatgaaa aaatttaaat ggtcgtatct ttatttaggg atcgtctttt tattcttata
    13321 tgcaccgatc ttctatctga ttttttattc cttcaatgaa ggaggaacga tgaacagctt
    13381 tacaggattt acttgggaaa attatgaagc tgtttttgaa gatacacgtt tgatcattat
    13441 cgtattgaat acgttcatgc tggctttctt atctgcattg atcgccacga tcatcgggac
    13501 atttggggct atgggaatct attacacgaa aaaacgacgt gcacggacag cgttgttgag
    13561 catgaataac atcttattag tctctcctga tgttattatc ggtgctagtt tcttgatttt
    13621 ctttacacta ctaggattcg gattaggttt tcagagtgta ttgctttccc atattgcttt
    13681 cagcattcca atcgtcgtct tgatggtctt gccaaaattg caggaaatga acgattcaat
    13741 ggtcgatgcg gctagagacc ttggtgctaa taatttccaa gtagtcaaaa atatcatttt
    13801 gccttatctt tctccaggaa tcatagctgg atattttatg gcatttacct attctcttga
    13861 tgattttgcg gttacatttt tcgtcacagg aaatggcttt acgacgctct cagtagagat
    13921 ctattctcgt gcaagacaag gaatcagttt agaaatcaat gcgctaagtg cgttagtttt
    13981 cttgttctcc atgatattgg tcattggata ctactttatt agtaaagaaa accgtccaaa
    14041 acggctgaag aaaaacagaa gagtacgcga gggggtggca cggctgcgat gaaaaaatta
    14101 caatctctgt ttttaggtat tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa
    14161 cttgaaaaaa caagtggcat gagtggagca aaggtactta ccatctataa ctggggagat
    14221 tatatcgatc ctagtttgat cacgaaattt gaaaaagaat atggatacaa agtgaattat
    14281 gaaacatttg attctaatga agcgatgttc acaaaaatcc agcaaggtgg gacaaactac
    14341 gacatcgcta taccaagtga atatatgatc caaaaaatga tcaaagaaaa actagtactt
    14401 ccattagatc attcgaagat caaaggatta aataatattg atccacgatt tttagacttg
    14461 gattttgatc cagataatca gtattcgatt ccatattttt ggggaacttt agggatcgta
    14521 tataacgata aattctttga tgcaaatcaa attaagcatt gggatgattt gtggaagccg
    14581 gaattaaaag acagtttgat gctgattgac ggtgcacgag aagttatggg gctttctttg
    14641 aatagtttag gctattcttt gaatagtaag aatatggcac agttgacaga agcagcaaat
    14701 aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat
    14761 atgatcaatg aagaagcctc cgtggcagtg acattttctg gcgaagctgc cgatatgatg
    14821 tgggaaaatg aacatttaca ttatgtgatc ccaccagaag gatccaatct ttggtttgat
    14881 aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt catcaacttt
    14941 atgctagaac cggaaaatgc ggcacaaaat gcagagtata ttggttattc tacaccaaat
    15001 gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg acgaacaatt ctatccatct
    15061 gatgacacga tctcccatct tgaagtctac aaagatctag gatcgaaata cctaggcatc
    15121 tataatgacc tattcttaga attcaagatg tataggagat aatggcaaaa ctcagtaaga
    15181 atggggactt atcaacgttc taaagagcta aaaatcgtcc tttttctatc tgagttttcc
    15241 aatcttctac caaaagcgga tgaaacgccg ctttttttgt gttattttat tttttctacc
    15301 acgtttttct accagagaaa agtggacaaa accattgtaa aatagggccc gagacgggct
    15361 agtaaatcta agattgaaaa agttacagaa ggataaagta attg
//
LOCUS       DACDSR010000061        15070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDSR010000061 DACDSR010000000
VERSION     DACDSR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.061"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..863
                     /locus_tag="IWK15_001922"
     CDS             <1..863
                     /locus_tag="IWK15_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547499.1"
                     /translation="IYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFI
                     RKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLT
                     AFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPL
                     VETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKK
                     NGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAE
                     ALENAFGFKE"
     gene            1437..2006
                     /locus_tag="IWK15_001923"
     CDS             1437..2006
                     /locus_tag="IWK15_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9547500.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1993..2853
                     /locus_tag="IWK15_001924"
     CDS             1993..2853
                     /locus_tag="IWK15_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9547501.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3043..3747
                     /locus_tag="IWK15_001925"
     CDS             3043..3747
                     /locus_tag="IWK15_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547502.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3752..5587
                     /gene="walK"
                     /locus_tag="IWK15_001926"
     CDS             3752..5587
                     /gene="walK"
                     /locus_tag="IWK15_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9547503.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5584..6900
                     /locus_tag="IWK15_001927"
     CDS             5584..6900
                     /locus_tag="IWK15_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547504.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6901..7770
                     /locus_tag="IWK15_001928"
     CDS             6901..7770
                     /locus_tag="IWK15_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547505.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7825..8634
                     /locus_tag="IWK15_001929"
     CDS             7825..8634
                     /locus_tag="IWK15_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9547506.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8737..9366)
                     /locus_tag="IWK15_001930"
     CDS             complement(8737..9366)
                     /locus_tag="IWK15_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9547507.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9536..10828
                     /locus_tag="IWK15_001931"
     CDS             9536..10828
                     /locus_tag="IWK15_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9547508.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10904..11566)
                     /locus_tag="IWK15_001932"
     CDS             complement(10904..11566)
                     /locus_tag="IWK15_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9547509.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11791..12075
                     /locus_tag="IWK15_001933"
     CDS             11791..12075
                     /locus_tag="IWK15_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9547510.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12126..13751
                     /gene="groL"
                     /locus_tag="IWK15_001934"
     CDS             12126..13751
                     /gene="groL"
                     /locus_tag="IWK15_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9547511.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13840..14988)
                     /locus_tag="IWK15_001935"
     CDS             complement(13840..14988)
                     /locus_tag="IWK15_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547512.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
BASE COUNT         4982 a         2558 c         3150 g         4380 t
ORIGIN      
        1 gaatttacaa gtttttttat cctaaaaaaa ttaaactaaa taggttaaag ccttatcatt
       61 tacaagagtt ttatcaatat atgttaaatg ataactgtac agcaaatacg gttattcatt
      121 atcacgcatt tatacgcaaa gctcttcaag aggctgttat tacagaattg ataagtacaa
      181 atgtagcaga taaagtaaga aagccaaaaa agaaacaatt tgtttctcaa gtttataatc
      241 aacaagagat cttgaaatta cttgacattt tgtcagaaga aaaactctat ttggtagttc
      301 ttttaacagc attttatgga ttgcgaagaa gtgaagtttt aggattaaaa tggtctgcta
      361 ttgatttttt aaacaaaaaa atcatgataa accatgtaat catagaaaat ccagagaata
      421 tgtcacagtt aataaagaaa gatcaaacaa agaactcttc cagctatcgt acattacctt
      481 tggttgaaac gattgaacga gcattagtaa atcaagctaa atggcaacaa gataatcgta
      541 ttcttttagg agatgattat attttagaag ataatgagta cgtatttact atggaagatg
      601 gacggttgat gaagcctcaa tatgtaacgc atcgtttgag taagttgatt aagaaaaatg
      661 gattgaaaaa aattcgcttt catgacttgc gtcattctaa tgcttcaata atgttagata
      721 gtggccagaa tatgaaatcc attcaagaat ggctgggaca tgcatcgtat tcgacaactg
      781 ctaatttata cacgcattta accgcaggag taaaagatcg tgcagcagaa gcattagaaa
      841 acgcatttgg gtttaaagaa taaaaaaaca aaaaacaata tctttggggt atcgcttctt
      901 gtttttagaa tttttttgtt ggcaatttgt tggcagtatg ttggcaatga aaaaattcac
      961 aaactaaaaa cctatttata aagctttata aaactttttt cacaaataga gcatttggtt
     1021 aagagtcagc tgctctacca actgagctaa cggcccgtac aaaaagtact taataatagt
     1081 agcactaaga attttaaaat gcaagactac atttaaagaa aatcagaaaa atctttactt
     1141 ccttatagga aatcattcat agattattca catttgctcg ctattatata agcataccaa
     1201 caaacaaagt tcttgcaaga gaacaaaccc taacactttt ttcatttttt actcctttcc
     1261 cgccgtctcc ccggcgggtc tcctgttctt aacgataaaa gacaagaagg cttagccttc
     1321 ttgttttttg ttttcatgga atcccgctaa tcaaatcacc tagttaaagc aactagattg
     1381 aatgtttatc ataagtctga tatcatgttt atgaaatgaa aaaaggagcg gttatgatgg
     1441 aaaaaatgcg tcagcaatta atggagtggg ggaaggaact agaaacattc cgtctgcctc
     1501 actgggaaga actccctgac ttagaacttt atatggacca agtcagaacg ttagttgatc
     1561 gttatctttc acctgtgata caaggagaaa agcatccatt gcttacctct tctatggtaa
     1621 acaattacgt taaactaggt ttgataccag caccagttaa aaaaagatat aacaaagaac
     1681 acgtagcatt tttacttgca atcactacgt tgaaacaagt attgactatt cctgaaatca
     1741 aagaagggat attgttccaa ggaaaaacag taggaattcg tgaagcctat aatctatttt
     1801 gcgatgaaca agaagcagct gtgtggatgg tcagccaatt agcacaaggt aaatctcatc
     1861 cgcaaaaatt cgaccaggca acccctgtag aatatattgc tgtaagagcg gcaactttat
     1921 cgtttgcaat gaaacttctt gcggaaaaaa cgattgttct agaaaccgaa tatttaaaag
     1981 aggagaaaac aaatgaaaaa caataaaatc gcaattttag tagattcagg tacggatgtt
     2041 ccagaagaat tgataaaaaa atacgagatg tatatgatcc cattgaaaat tatttataaa
     2101 gatcaggtat atacagataa aattgacatt acccctgaag agatctatca acgtttgcca
     2161 gaggaaatcc ctggtacatc tttgccggat ggtgagtcga tcacagaaat ctttgagcaa
     2221 atcaaaaaag acggttatga aaaggtactt gcggtaacaa tttccagtgg attgagcggg
     2281 acatataatg tggttcgttt aattgcacaa cagcaagaag aattggaagc atatgtacta
     2341 gatacgaaaa atattggtat tggtgcaggt ttttctgcga tccaagcagc aaaatggctg
     2401 gaagaaggag tagaatggga tcaactgatc agtaatctga atgagttagt cgaaagaaca
     2461 aaagttttct ttaatgttgc gacattggaa tatcttcaaa aaggtggacg gatcggttta
     2521 gttgcttcta ttgtaggaac cgctttaaag cttaacccaa tcatctcatg taatgaagaa
     2581 gggatttatt acacagtagg aaaagctagg ggacgtaaaa aaagtttgga taaaacggtt
     2641 gcttacgtca aagaaaaaat cggtaatgca aaggtcttca acttagctgt tgcccatggg
     2701 gatgcgaaag aagaagcaat tgaaatggaa gcgagactta aaagagagtt cccacaagca
     2761 aaccaaatct actttggcca gatttctccg gcattagtag tccacactgg tccgggatta
     2821 ttgggtgtcg gtgtacaagt tttagaagga taaatcaaaa aaaggaaaca ctgaggtgaa
     2881 aatcagtgtt ttcctttttt attgaagtta gaacaaaaaa taataaagta ttttgcactt
     2941 tcattttaat tttctctttt gttttatagg caaagatgaa acaaaagcag aaatacggta
     3001 taatgagaag gtatgacaat aatatataag aggtggctct ttatgaaaaa agttttagtt
     3061 gtcgatgatg agaagccgat ttcggatatc gtcaaattca atttagccaa agaaggctat
     3121 gacgtttata ctgcatatga tggggaagaa gccttggaaa aagttgctga ggtagaacct
     3181 gatttgattt tacttgattt gatgctacct aaaatggacg gcttagaagt tgctcgtgag
     3241 gtacgcaaaa cttatgatat gccgatcatt atggtaacag ccaaagattc tgaaattgat
     3301 aaagtcctag gcttagagtt aggcgccgat gattacgtga ccaagccatt ttccaaccga
     3361 gaattagttg cacgtgtcaa agcaaatctt cgtcgcggtg ccacggctgc gaaggaacca
     3421 gaagaggcag cacctgcgga attgacgatt ggtgatttga cgatccatcc agaagcttac
     3481 atggtgacga aacgaggaga aacaatcgag ttaacacatc gtgaattcga actactcttt
     3541 tacttggcga agcaccttgg acaagtgatg acaagagaac atttattaca aacggtttgg
     3601 ggttatgatt attttggaga tgtacgaaca gtggatgtaa ctgtacgtcg tttgcgtgaa
     3661 aagatcgaag ataatccaag ccatccaaat tatttagtga cacgtcgtgg tgtcggttac
     3721 tatttgcgta acccagaaca ggagtaattg gatgaaaaga aaagtccgct tttttcgatc
     3781 agtcaatttt aagatcgcta ttacctttat tctgatttta ttgatctcga tcgaaattat
     3841 tggtgcctac tttatacgtg gtttagaacg ttccacgatc aatacattta ttaaagatat
     3901 gaatcaaacg gtggaatcat tagcaacaac aatcagcccg gaattgaatc gaaaagataa
     3961 tgcagatgat gaagaggtaa atgcgaatat caaaagattt attgaaaaca gtgcgactag
     4021 cgatatcatc gaaatacggg tcgttgacga aaaagggatc atccgaggaa caaccgatgt
     4081 caatgaacaa agcgctgtgg gaaagaaaaa tgattatgtt gatatcaatg atttcacgac
     4141 taaacgttat gttgctttag ataacgacga taaacgggtg aatatcaatg ttcaaccgat
     4201 cctgtccccg accggcgatg ccgttatcgg ggctctttac gtcaaaagta atatcgaaca
     4261 aaaatattcg gaaatcaata acacagcagt cattttcttt actgcctcac taattgcggc
     4321 atttatttca atgatcgtat ctgttttagt tgctcgttcg attacacagc cgatcggtga
     4381 aatgagagaa caagccattc gaatcgctag aggagactac agtcggaaag tcaaagtgca
     4441 tggacaagat gaacttggac agctggctga taccttcaat cagttagctg aacgtattga
     4501 agaagctcaa gatacaatgg aagctgaacg caatcgtttg gatagcgtgc tatcccatat
     4561 gacagacggc gtcattgcga cagatcgcag gggtaaagtc attacgatca atgatatggc
     4621 tgtttccttg ctagatatca aaaacgagga agcaatcgga caatcgattt tgacactttt
     4681 agatatcgaa gaagaatata cgttgcggaa attattagaa gatccaaacg aaatggtact
     4741 tgaccgttca accagtacat tggaaagtga tcagatgatc ttgcgagtag acttttctat
     4801 gatccgtcgc gaatcaggtt ttatcagcgg attagtagct gtcttgcatg atgtgacaga
     4861 acaagaaaaa acagaacggg aacgtcgtga attcgtctct aatgtttccc atgaacttcg
     4921 tacaccgttg acaagtatgc gtagttatat cgaagcattg agcgaaggtg cgtggaaaga
     4981 cgaggaagta gcaccaaatt ttttgaaggt aacgctagat gaaacagacc gaatgattcg
     5041 aatgatcaat gacctcttaa atctatctcg catggataca ggtaataccc agcttcaatt
     5101 agaatatgtc aatttcaacg agttagtgaa ttttgttctt gaccggtttg atatgatggt
     5161 gggaaatcag gaaaaaaatt ataagatccg tcgtgaattt acgcaaagag atctttgggt
     5221 cgaagtagat acagataaaa tcatccaagt agtcgataat atcatgaata acgcgatcaa
     5281 gtattcacca gatgggggaa cgattacttg tcgcttatta gaaacgcata ataacgtgat
     5341 tttaagtatc accgatcaag gattggggat tcctaaaaaa gatctgaacc gagtgttcga
     5401 gcggttctat cgagtagata aagcacgtgc aagagcacaa ggtgggacag gacttggatt
     5461 agctatttct agagaagtga ttaaggcgca tcgaggcgct atttgggcag agagcaagga
     5521 aggaaaaggg tcgacttttt atatctcgct gccatatgaa ccttatgagg aggaatggtg
     5581 ggaatgagaa tctctgaaaa agtcgttcgt ataggactca ttttgatggt tgcgttaagc
     5641 atatacttct cttatgcaat ctggctgagc ccagcaggaa aaagttcaat caacgtagat
     5701 gagacaaaca ctcaaatgat cgagtctcaa agcaatcgaa aaacttccga agtctttctt
     5761 ccgcttcatg ttacatggca ccattcggga atgatccaag aaaccaacag tgaaaatctc
     5821 gtgtaccgat tgcaattgat catcgataaa gcaagatttg gaaagctttc tgaagtagtt
     5881 tcaggagaca aatcacagtt tgaacaggtg aaaaatctga actctggttt tgagttggcc
     5941 tataatgcgc cttttttgct aagagaatac aaggaagcat ttgatttaga tttagatgtg
     6001 tcgaacttgg caaaagaaag cgaaaatatg tattttacta gaatacaatt cgatcaagga
     6061 aataaaaaaa tccgattttt gaattattcg aaccatctcg tatatgaagc aaatatttcg
     6121 ttgaattgga cagatatcaa taaagaatta acagcaaaag acacaaaatg gacagaaatg
     6181 acaacagaac catcgacgat cgacacgcaa tatgctacga aacattcgat caaactgaag
     6241 aagtatagtt atatcctgtc acagcaaccc tatacacttt ttagaaatgc ttttttccag
     6301 aaaccagata gcgtgaaaaa taatgatgaa agcaatgaat tgatttttta cggtgggaat
     6361 gaaacaatgt ctatccattc ggaaacgcaa gtagttgatt ttcgtggaga gctgccagag
     6421 aaaaaagaaa atgctagtgt attttctgaa agtttcccct atattagcaa attaggcagt
     6481 tctttaggga acctcagata ttttgaccgc agtgacaata cgatcgatta tcggatattc
     6541 gtggaaggct tcccggtttt tgggacagac agcagaggac agattggtgt ggaagttggc
     6601 ggtgaaacga atggttctgt gcaagtgaat atccaaacca gtttgaatac gattcaagtg
     6661 ccgatacctt cagacgaaga agtagaactt gcaagtacga atgaaatcat ccagcagtta
     6721 aaagctaagg gagcagatag caacaagatt ggttcagtga tcgttggcta tacatggcaa
     6781 aatgtcaaag aaacgaatcg tgtcgttgat ctcttacctg aatggtatgt gaaatataaa
     6841 gataactggt attcagctaa cgaccttctg gaaaaattac ctggattgga ggcgaattaa
     6901 ttggatttca aaaaaatcga atggatattt ttcgtcgctt ttttagggtt gaatgtgttt
     6961 ttattcagca gttatcatga cgcggtatac caagaaaaca atgtcattcg ctctaatcag
     7021 actgaatcaa tccaacaacg tctggctagc gacgatatca cttacacggg gaagatttcg
     7081 tctgagaaca agcaaggcta ttatcttagc gcggagcaga ccaatcttag cacttcatta
     7141 gcgaattatc gcaaggctgg taaaagcggc ttgctggcaa gtggtgtgtt tttagacggt
     7201 gatgttttga cgaaatcttt atccagttca gatgctgaaa agaacgtgat cgatccagat
     7261 aaaattagta gttccttgga agatttttta tctaacaaag acaatatttt attcggtaat
     7321 gattatgtgt acacgaaaaa cttttctcat ttagaagaag aaccactaga aatccaagca
     7381 agccaagagt atgaaggcat cccattcaat gatgatacag caaaaatcgt tctttcttta
     7441 gaaaaaaacg ataatgatta tagcatcacg aaatacatgc agacgcattt atctgatatc
     7501 gagcagctgc gcgaaaagac ggaactgtat acggaagagg aagcaatcaa tacattgtac
     7561 atcaataaca aaataccaag aggatcaaaa attttatggc gtcagctagc ttattcctgt
     7621 attctgaaag tccgggaaaa aaatgtgtat gttcctgttt ggcatgtagc gatcgaaaca
     7681 agcgataaag tggtccaaat tgagagcgtc aacgctttca gcaatacgat cgtgacaaac
     7741 aatactgtac caaaagtgga agatcaataa aaaagcgagt ataatgaaaa aagtttacaa
     7801 agtaagaagg gaagtaggag tcaaatggct gaagaaaaac cagcttttaa aatcagtatc
     7861 ctagcaagtg gaagttcggg taattcactt tatatcgaaa gtgaaaaaaa acggcttttg
     7921 gtcgatgcag gacttagcgg taaaaaaatc acttccttga tggctcaaat tgatcggaca
     7981 ccggaccaat tagatggaat acttgtgacg catgaacatc gagatcatat acatggcgtg
     8041 ggtgtcttgg ctcgtaagta tcatttggat gtttttgcca atcagcagac ttgggaagcg
     8101 atggaaccac tgattggaaa tgtaccagtg gaacaaaaac atctatttga gatggggaaa
     8161 gtcagaacat tcggcgatat cgatattgag agtttcggcg tctcccatga cgcagcggct
     8221 ccgcaatttt atcgtttcca taaagaaaat cggtcattcg tcatgttgac ggacaccgga
     8281 tattgcagtg attatatgcg aggtgtcatt gaaaatgccg atggttattt gatggaaagt
     8341 aatcatgatc ttgagatgtt acgcatggga ccttatccgt ggaatttaaa acagcgtatt
     8401 ttaggagata gaggacatct ttccaacgaa gacggtgcac ttgtcatgac tgatgtgatc
     8461 ggtaatcgga ccaaacgaat ctatcttggc catcttagca aggaaaacaa tatgaaagaa
     8521 cttgctcatt taacaatgga aaatgttttg aaagaaaaag attttggcgt tggacatgat
     8581 tttgaaatct atgatacaga tccagatgaa gcaactgagc tattttcgat ttaaacagtt
     8641 caatcaataa aaatataaaa agcagaagat aaaatcaatc agggttggtt ttatcttcta
     8701 tttttatttt tttagcatat taaaaaaata accgtattat cgactgacta cactgatgtg
     8761 ctgcttttca tgtttctcat ccagtatctc atctactaat gcaatcgcgt aatctgccat
     8821 gctgatcgta ctgtcgctag cagcgtttct ttgtaaataa tcatcactta acaaataagt
     8881 acctgtacgt ttgccatgtg gatcaaataa agaagctgga ctgatataag tccaatgtag
     8941 atttttagat tgtttcaatt ttaacaatcc ttcgtacata ttgaaagctg ttggatagaa
     9001 gggtgcattt ttaggtactt ggtcaatcat acgagtcgtc ttttctgtat ctaagaataa
     9061 cgaagaggca cccccaacga caattaagcg tgtatcggtt ccttcaagca tctcaatcaa
     9121 atgggacaaa gaagtttgat gcatttcttc ttttcctttg ggagcgttga aagcatcaac
     9181 gacaacatca aaagatgcaa gatcagtagt tgtcaactca aataaatcct tttgtataaa
     9241 cggaacatct gatggcaatt tttctggatg acgcacgatt gccgtcacgt ctaaatctct
     9301 ggacaatgct tcgtctaaaa taaatgaacc tgcatggcca gtagctccaa tgagtgcaat
     9361 tttcataaaa aacctccttt tttattcctg tccattgtaa gcagttgctg aatattcgtc
     9421 aaagaatccg atttttgaaa agctcagtgc gcattccttt catggttcgt gatcattggg
     9481 tatagttaaa ttaagaaaac tgattgataa aaatagaagt tgaaggagat aagaaatgaa
     9541 agagtggtta gatcgtgcct ttttaccaat gataacatct gttgtgaatc ttagaccctt
     9601 aactgctttg cgtaatggaa tgacttacac gatcccaata attattgttg gctcgctgtt
     9661 tttcttattt gctcattttc ctgtggatta catcgctcag tggatagaca aaacaggatt
     9721 ttctatgtat tttacacaag tataccaggc tacatttgat gtgatagcct tttggatcgt
     9781 ttttgccatt gcatacgctt atgcaaaaga agaagacatc gatcgcttac cagtcggcat
     9841 aacagccttg tctgcttatc tactgcttat tcagccacta aatactcaga tagatactgt
     9901 ctggacaggt gctgaaggga tactaggagc agttacaact gggctcatca tcggatgggg
     9961 ctatgcacgt ttagtaagac ttgcgaaagg gtggaagcaa agagagaaga tacctgaaaa
    10021 tgtttggcag tcttttactt cattgattcc aatcattgtt atagtcactt ttacgcttat
    10081 cggttcagcg gtttttcaac atttgttcag aatgactgtt gttcaaggag tatggacata
    10141 tgtccagctt cccattcaat cgttcataga cacgttgttt ggtgtgcttt tattaggttt
    10201 ttttgttccg tttttatggt tatttggtct tcatggatct gcgatggtca atgggttggt
    10261 tagtcccgtc ttgcaagcga attcattagc aaatgcagaa atcttagcct caggacaaga
    10321 gttgacggtg gcaaatggcg gacatatcgt tactcagcaa tttctcgatc agttcatgac
    10381 ggttacgggt gctggattga cgttaggggc tgtatttttt atgacggttt ttgcgaaatc
    10441 gaagaaatac agagaattag gaaatcttgc tcttttacca gcctttttca acatcaatga
    10501 atcaattatt ttttccacac cgatcgtgat gaacccgatg atggctgttc cttttatttt
    10561 cgcaccggtc gtttcaggat tgattaccta ttccgcttta tattttggat tcgttccttt
    10621 atttacagca gtccaagtcc cttggacaac accgccgatc ctctcgggtt tcttagtagg
    10681 cggagtgcct gcagcgattt tacaaggtat cgtgttgtca attagttttt tcatttattt
    10741 cccatttttg aaaaaaatag attctgcaaa ctgcaaaaag gaaagacaga caaaaaatga
    10801 tggagcagca gaaaagatga acgactaaaa aacaaaaata ctcctgtcgc tttagattgg
    10861 gaaaatcacc aatctaggat gacaggagta tttatagtca tgtttatact agcccttttt
    10921 ggacagctaa ttgaacaaga acaactaatg tgttcattcc tacgtgtgtg atcatcgatg
    10981 tccagatccg accagttgct ttgtactcta ggctgaagaa gaaacctagg aaaaagtaga
    11041 ttagcagatg accatcatta tgagcaaacg caaacagcaa agagctgatc actgctccga
    11101 cccaaaaatt tgaatatcga ctgatgatcc ccagtatact gcggcggaat acgaactctt
    11161 ccatgatggg tccagcaaca gttgtcgcca aaataaaagc gggtgcttcc atgatcatct
    11221 ggatgatatt ttgtgtattc tcggaagggg ttgctttgcc aaacaataca gattctaaca
    11281 tgacagcgat gctttgcaga atcaatgcga caaagattcc aaccaatcca taaataacaa
    11341 tctttggagc agaagctttt tttaccgctg attcgatcgc taagggttca cgttgcttaa
    11401 aataaaaaat agctaggaca acggcgccta gtatatacat gactgcagtt gctgtgatca
    11461 attcagacgt agattttaca agtcctatcg aagctaggat caagggtgag ataaaaatta
    11521 gaccatagca aaaaatgctg aggaagctat atttttttaa agacatgtaa ttcctccttt
    11581 taagggataa caagcatgca aggcaagctt atatcgattg atttcaatta tatcataatt
    11641 tcaataagca gctgtcagaa taaaatttct tttctttgat ttaaaagctt gcaaaaaaga
    11701 gggagaatga gtattattaa aaatgtaggt tagcactcga aggttaagag tgctaacaga
    11761 ttaaaaaaag agttatcgga gggatttatc gtgttaaaac cattaggtga tcgcgtcatt
    11821 atcgaagtcg caaaagaaga agaaaaaact gtcggaggaa tcgttttagc ttctgctgct
    11881 aaagagaaac cccaaactgg gacagttgtt gcagtcggtg aaggccgttt gttagaaaac
    11941 ggtgaaaaag ttcctgccgc tgtaaaagca ggcgaccaag ttatgttcga aaaatacgcc
    12001 ggtacagaag taaaatacga aggaaaagaa tatctgatcg tcgcaggaaa agatatcatg
    12061 gcgattgttg agtaaatttg gaagataaaa aaataatcaa aaataatcaa tgaggtgaat
    12121 caatcatggc aaaagaattg aaatttgcag aagacgcacg cgcagctatg ttacgcgggg
    12181 tagataaatt agcagataca gtgaaagtaa cattaggacc taaaggccga aatgtcgtac
    12241 tagaaaaatc ttacggctct ccattgatca caaatgatgg tgtgacgatc gcaaaagaaa
    12301 tcgaattaga agatcatttt gaaaacatgg gtgcaaaact tgtatcagaa gtagcatcaa
    12361 aaacaaatga tattgcagga gacggtacaa cgacagcaac tgttctgaca caagcaatcg
    12421 ttcgcgaagg tttaaaaaac gtaactgcag gtgctaatcc tctaggcatt cgtcgcggga
    12481 tcgaattggc aacaaaagca gcagtcgaag aattgcataa tatctcaact gttgttgatt
    12541 caaaagaagc cattgcacaa gtagcagctg tttcttcggg ttctgataaa gttggacatt
    12601 tgattgctga tgcaatggaa aaagttggaa acgacggtgt catcacaatc gaagaatcaa
    12661 aagggatcga aacagaacta gatgttgtag aaggtatgca gtttgatcgt ggttacttat
    12721 cacaatatat ggtaacagat aacgacaaaa tggaagctgt tctagaaaac ccatatatct
    12781 tgatcacaga caaaaaaatc tctaacatcc aagatattct gccattgtta gaacaaatct
    12841 tgcaacaatc acgtccattg ttgattattg ctgacgatgt agatggtgaa gcattgccaa
    12901 ctcttgtatt gaacaaaatc cgtggaacat tcaacgtagt agctgtaaaa gctcctggtt
    12961 tcggcgatcg tcgtaaagca atgcttgaag atatcgctat tttgacaggt ggtacagtaa
    13021 ttactgacga tctaggttta gaattgaaag atgcaacaat cgaaaactta ggaaatgctt
    13081 ctaaagttgt tgtagataaa gacaatacaa cgatcgtaga aggttctgga gaaaaagaag
    13141 caatcgaagc tcgtgtgcaa ttgatcaaaa accaaattgc tgaaacaact tctgattttg
    13201 accgcgaaaa attacaagaa cgcttagcta aattagcagg tggtgtagct gttgttaaag
    13261 ttggtgcagc tactgaaaca gaattgaaag aattaaaatt acgcattgaa gatgcattga
    13321 atgctacacg tgccgcagta gaagaaggaa tggtctcagg tggtggtaca gctctagtga
    13381 acgttatcag caaagtttct gctgtagaag cagaaggaga cgtggcaaca ggtatcaaga
    13441 tcgttgttcg tgctttagaa gaaccaattc gtcaaatcgc tgaaaacgct ggttatgaag
    13501 gatcagtgat tgttgataaa ttgaagaacg tggaactagg cactggtttc aatgctgcta
    13561 ctggagaatg ggtaaacatg gttgaagcag gtatcgtaga cccaactaaa gtaacacgtt
    13621 ctgccttgca aaatgccgct tccgtttctg ccttgttatt aacgacagaa gcagtcgttg
    13681 cagataaacc agaaccagca gcgccagcag ctcctgcaat ggatccatca atgatgggcg
    13741 gtatgatgta gtaattaaga aagaacccta ttttaacagg gttctttctt ctttttttgt
    13801 tttattttaa gcaaaagggg caaagaaggg gcaaaaatat caaaggctat ttagtttatc
    13861 aattacttgt tgtttggctc tttttgttac atggttataa atagacagag ttgtgtttgc
    13921 atcagaatgc cctacacgct ccataatggc tttaagaggt acgcctaatt cagataatag
    13981 tgaaacgtgg ctatgtctga atatatgtga ggatagactt ttttctaatt ccaactcttc
    14041 ctctacttta tggagtatag cgttaaatga gtgcagcgca agcggagtgc cacttgtaga
    14101 tataaatata tattgatcgg gatctgtggg tctacctgca agaatattgt cagctatcac
    14161 actttcaatc aattcttttg cacgattggg taattgcact tcacgttgcg aataagtatt
    14221 ttttggagtt gtttttatag cattatccat tttcacagat gtgtaatcta aggtcccatt
    14281 aatggaaatt ttcccatcct catagtcctt catttgcaaa gctagcaatt ccccatatct
    14341 caaaccagtt aaatatagaa actcagctat tatgccgtgt agttttctgc gaggattgga
    14401 gtatagctgt ttcagtattt gatcaatttc ttctttctcc agatattttt tattcataga
    14461 aagccttctt ttttcttctt ccactttttt aggatggatt ttaactgcta gcgcagggtt
    14521 tctttgaatg tattttctat cgattgcata gtttagcata acagatagag ttgtttttgt
    14581 ttgttttgtg tagttcagtg agaggtcacc aaacgtatac atatcttcaa ttatcttatt
    14641 aatgagtgtc tcatcaatgt ttctaacgat cgtatcatcg cttatgtgct tagaaacatg
    14701 tttcatcatc attggaacct tcaaatagct agtacgttta acatgctgct tataatattc
    14761 ataccattct ttatacagct caccaaaagt gatatctgat ttattgtaat cttcgagtgc
    14821 ttctttaatt tttttatcta gaattttctg agcttttttc cacgcttgtg gtgaattact
    14881 tgtaagtgtt gtagattttt ttcgtgtttt ttctgtataa ggatctacat atctttcaat
    14941 aaacttgaat tggccatctt tggtctgttc aacccacact tttaacatct cctatcattt
    15001 gctataatag gcataacaaa tagacctata taggtttgtt ttctaaaagc acgctcttac
    15061 tctggacggt
//
LOCUS       DACDSR010000062        14848 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDSR010000062 DACDSR010000000
VERSION     DACDSR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.062"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(329..1057)
                     /locus_tag="IWK15_001936"
     CDS             complement(329..1057)
                     /locus_tag="IWK15_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547513.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1035..2609)
                     /locus_tag="IWK15_001937"
     CDS             complement(1035..2609)
                     /locus_tag="IWK15_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9547514.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2838..3776
                     /locus_tag="IWK15_001938"
     CDS             2838..3776
                     /locus_tag="IWK15_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9547515.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3788..4699
                     /locus_tag="IWK15_001939"
     CDS             3788..4699
                     /locus_tag="IWK15_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9547516.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4723..6195
                     /locus_tag="IWK15_001940"
     CDS             4723..6195
                     /locus_tag="IWK15_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9547517.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6246..6857
                     /locus_tag="IWK15_001941"
     CDS             6246..6857
                     /locus_tag="IWK15_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9547518.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6887..8800
                     /locus_tag="IWK15_001942"
     CDS             6887..8800
                     /locus_tag="IWK15_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9547519.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8928..11909
                     /locus_tag="IWK15_001943"
     CDS             8928..11909
                     /locus_tag="IWK15_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9547520.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11906..13567
                     /locus_tag="IWK15_001944"
     CDS             11906..13567
                     /locus_tag="IWK15_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9547521.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13607..14539
                     /locus_tag="IWK15_001945"
     CDS             13607..14539
                     /locus_tag="IWK15_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9547522.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4824 a         2342 c         2984 g         4698 t
ORIGIN      
        1 ttgtcttcac caacacgatt tgacgaagag cctatttaaa taactacatc catttattct
       61 atcttctaaa atagcctttt tcatttcagt taaatagttc agcgccattt ttgcactcga
      121 cttgcatagt agttttttcc atattcctca acctcagcgg gggttgaaga cataaggaaa
      181 atcctatgcg ttatgctcaa ctggatctga ttaaatattc taatgtattt ttctctacca
      241 tcattaaatg gttaatagcc aaaaatcacc acttttcaat ggctccttgt tgtataagta
      301 taactagaag atgaacacgt ctgagaaatt atagaagctt tttacggtaa ttactggggc
      361 tgtgtccagt agtttttttg aagagaacag aaaaatagct cacgtgatct attcctactt
      421 tttgactaat ttcatcaata gataagaatg tgtctttcag tagagcactt gctcttttga
      481 ttcgaaagtc tagcaagtaa tcatgaaagg aataacctgt ttcttttttg aaggcccgtc
      541 ctaaataagc gggatggtaa tggaattgtg tagctacatc tttgagcgta agcttttcac
      601 tataattgtt ttgaacgtaa tcaatcactt gttgaattga ctcattaaaa gaatcatcag
      661 ttagagagtt ttgattagct gctgatttac gcattttttc ttcttgtact aacttaacta
      721 aataaggaaa atcaatgggt ttcgttatgt aacttttaat agataaatca atcccctttt
      781 ttgcatactg aaaattttgg tgggcactaa tgacaatact agtaaagtag cgattggatg
      841 tttgttgcaa agattcgatt aattcgagtc cagaaatttt ccccatcgta atatctgtga
      901 taagtaaatc aaagcgctgg ttttgcaggt ttttaaatgc ttcgtctccg tctgtgtagc
      961 agtgatcgat aatgatgtcg gggactttct tcaaaagata atgccttatg ccttctacaa
     1021 ttgtacaatc atcgtcaaca attgcaattc tcaacatgca tattcccccc tttttgtatt
     1081 ttgatagtaa tcgttgttcc gacgtgtagt tggctttcta taacaaaacg tgtatctcca
     1141 ggatagagat gctttatacg tcgaaaagaa tttgagatgc cgatattctc actataagga
     1201 atatcatcat taagtttttg ttgaatatgc tccagcttgt cttggtccat tcctttccca
     1261 ttttcaacga tttttaacac ataatggttg gttgtttcag ttgcatagac agtcaaacga
     1321 ttttcctttt ttgatgaatc gaagccgtgt ttaatataat tttcaacaaa tggctgtaaa
     1381 gaaagttttg gtaacattaa ttgatcaata tgatcaatca gctgatagtc aaagttatgt
     1441 cgataacgac gttgatgtag ttgcaaggag aggtcgatat taattaattc ctctttcaaa
     1501 tcaattatgt cgggctgata tgtattattg cgatacaact tgctcaataa atatatactg
     1561 tcgctgattt cttcgtcatc attcaatatg gcagtcattc gaataaattc caaattatta
     1621 aatagaaaat gaggctcgat ttgatattga agtgctgcat actttgcttc ttgcttcaac
     1681 caattatttt cataaagtgt tttttgatat gtagcattat ctaaataaaa ctgattgata
     1741 tgcatagcga tttctttcaa atcaccgact ttgttttcga tattgattga cttgtagtct
     1801 cctgtagatg aatgatttaa ggaagtaatg atttcctgat actgctggat atagttacga
     1861 aaacttctat ttaataattg atgaatagca agaataaaca aactcattaa taagaaaatc
     1921 atgattgcca gacctaataa ggtttgtgac gaataatttg gattttcata tacttgtaat
     1981 gtgtggttac cgatagctat ctgttgcgtt tttttccatt tagaaggcga gatgtcttca
     2041 gataaattga cggaatcaat accattagac aaagaataat tgtaatgtgt tggaaagaga
     2101 ctctttttta gcgcgttgat cgaaacgtct agaatcaatg tagcatcggg catgtcctca
     2161 ctaatgaaac ttaatgaacg aataaaagaa attttttcgt tttttgtagg tgcgtaggca
     2221 cttaagtatt tttcgtaatc taatgagata gatatatttt taacttcatc atcttgcaca
     2281 agttttttta agttatctac taaataggta ggggagcgat cctttgacaa aatatcttcc
     2341 atgtattcag caggtgatga gaggtagtag tcattaaggt tttcttggaa atcataattt
     2401 tgatagatct ctgtcacgat accggagaga atacgattat atgtttccat atagtgggtg
     2461 atttgctgaa tgttttgcag gttttgattt ttttcttttt caatcacatt gatataggaa
     2521 aagaacaatg tgaaacaggc aagtaaagta ataaaaaaaa tcaatatgta tgtaaaacgc
     2581 ataataactt ttgataaatc gtttttcatt caaagtcctc attttgataa tctctaattt
     2641 tatgaatcat tatagcacat attacaagtg atgtaaacgc tttgcaaaca gggctactta
     2701 tgattgtgaa tacagtttcc gtttaaaaac tattctattg tagaggttta ataaactaaa
     2761 aataatgtat aaaaaattaa tgtaagcgat taaccaagtg ggtataatca aggaaacgct
     2821 gagagaggag agacaaaatg aagctaaaga aagggttatt aggaaatcta aaaaaaaacc
     2881 gagcattgtt tattatgctt ttaccaggtg cgttatggtt atttatattt tgctatatac
     2941 caatgtttgg agttgtaatc gcttttaaag atttcagaat ttcatcaagg ggattcttta
     3001 caagtttgtt caacagtcct tgggttggct gggaaaattt caaatatctc tttgcttcgc
     3061 aagatgcttg gatcattact cgcaatacgg ttgtttataa cgttgtaggt attttgctaa
     3121 gtactttttg tacagtgtca ttagcaatca ttctgagcca aatggcgaac aaacggttat
     3181 tgaaaaagat gcagacattt atgatttttc ctaatttttt atcatgggta gtcatcagtt
     3241 actttgttta cgcatttttg agtacggata aaggattaat taatatttta ctaagcaatt
     3301 ttggtgtgga acccataaat tggtatagtg aaccgaagta ctggcctttt attattgttt
     3361 ttacgagttt atggaaaggc gtaggctatg gatccatcat ttattacgcg tcaattatgg
     3421 ggatcgatac tagttattat gaagctgctt tagttgatgg tgccagtaaa tggcaacaga
     3481 taaggaatgt agtcttgcca catttaagat cgattattgt cattatgttg attttgtctg
     3541 ttggtagtat ttttcgagct gattttggat tgttttatca attgccaaga aattcggggg
     3601 ctctttatga tgtgacgaat gtattagata cttatgtcta ccgtggatta atgaatagcg
     3661 gagatatcgg aatgtctacg gcagcgggat tatatcagtc atcagttggc tttgtcttga
     3721 ttttgattac taataagata gtcaacaagt tagatcctag ttcagcacta ttttaggagg
     3781 aatcaaaatg atatcaaaat taaaacggca gatgtacttt tcgctgccag tcgaacggtt
     3841 tttcacaata ttactaatct tatttactct tagctgtatt tttcccttta ttttcgtaat
     3901 cgtcatttca ttaacagatg aaaaagcatt acaactcaac ggttacgaat tattgccggc
     3961 tcaatggagc actgatgcat atcaatactt aatacaagat ggcggacagt tacttcggtc
     4021 attaggcgtt acaattatga ttacagtgat tggaacgttg attactgttt ttatgacggg
     4081 aacctatgct tatgttttat caagagcctc tttcccttac cggaagttct ttacttttta
     4141 tctttttttt accatgttat ttgcgggagg gatggttcct tcttacttag tgatgacgaa
     4201 gatgcttggc ttaaaaaata caatttgggc tttgattcta cctcttgctt ttagcccata
     4261 caatgtcatt attttaagga cattctttaa gaagtcgatt ccagaatcga ttatcgaatc
     4321 cgctaaaatg gatggctgta gtgagtttcg agtttttttc caaatcgttt tgcctttagc
     4381 gatcccaggg gttgcaacaa taggtttatt ttcaagctta ggttattgga atgactggtt
     4441 caacgcattg ctgtatattg acaccaacaa attgattcca ttgcaatatt tgttaatgaa
     4501 gattcaaaat tcaatggaat tcttagcaaa taataatgac attacattag cgcagcaaca
     4561 agcaattcaa aatagccttc ctcaagaatc aacacgaatg gcaatggtcg tagtagctac
     4621 tttaccgatt gcaattgttt atccgttctt ccaaaaatac tttgttcaag gattgacgat
     4681 cggtggagtg aaagaataaa aaagaatagg agatgttaaa agatgaaaaa gaacatgcag
     4741 aaatatttga ttggtggtgc ttctgtacta acccttggcc tattaggtgc ttgtggtagc
     4801 agtaatggcg aagcaaataa tgaaacagga accacaaaag ttacttggta tcaggttgga
     4861 gaagaaccca aagattttga tgaggtcatg acaaaagtca acgaaaaatt aaaggaagaa
     4921 atcaatgtag agttggatat gcggctgatt ccggacgggg attatcagca aaagcttggt
     4981 gtgatgatca attcaggaga agaatatgat atttgtttcg tcaatggcac agattatgtg
     5041 aattatggga acaaaggtgc tttcattagt ctcaacgatc atttggataa agagttgaaa
     5101 gattatgcag ctgaattgaa tgaaggattt attgaaggtg gggcaattaa tggcgaaact
     5161 tatggattgc cagtcaataa cgatattgcc acaacggaat attggacctt taataaagat
     5221 ttggtagatc aataccaatt ggatgtagag aatgttgaag gtgtagaaac attggctcca
     5281 gtattagaaa agtttgcaga aaaaaatact gatacgaata ttacgccaat cggtatgacc
     5341 cgtaccagtg tctgtattcc ttatgacaat attatcggtg gtggtgtacc gctagcggtt
     5401 gcatttgaag gcgatacaca aacagttgtt aatacttatg agactccaga aatgatgact
     5461 tatttgaagg ctatgcatga gttttataaa aaaggatata ttttgaaaga tgcagccact
     5521 gttacgacag atccatttcg aattgatagt aaaaactggt ttttaggtcg taactcaagt
     5581 ggttatcaac cattagcaca aaaaaaatta gaagctctag cgggacatgg attaatttta
     5641 aaaccagctg gaaaagcttt taaaacgact ggatttgcaa gagtggcaat gcatgcggta
     5701 tctgcttcaa gtaaacatcc ggaagcagcc ttgaaattgt tgaattatgt gaataccaat
     5761 caagaagtag cggatttaat ggtttatgga attgaaggaa aacactatga tcgcaatgaa
     5821 gatggttcga ttactttaac aaactctgat gattattatc aaggcgcttg ggcattggta
     5881 aatacttatc tagtttcaaa aacttcaccg attacagaag aaagtaaaaa actagaagaa
     5941 gaaagtaaaa agtttcaaaa tgaagcaccg atgtctccta ttttaggttt ctcttttgac
     6001 gcttctgatt ttcgaacaga aattgcaagt gtacaaaata tcgtgtcaca atatgaagta
     6061 cttttaagct cgggaacagt tgaccctgaa caatatgtac cagagttgtt agaaaaacta
     6121 gaaaaagctg gattggataa aattcaggca tctgctcaag aacaatacga tgaatggcga
     6181 aaatctaaca aataattttt tactaaatga aaagaataga ctaagcagtt agtgggggga
     6241 ttcaaatgaa cgaaaacaaa tggaccaaag gatttaattg ggtttttcaa ttgttgttac
     6301 taaactttac ttattttata ctaatttgtt taggatttgg aattttaggt tttttccctg
     6361 ctacgatagc tttgattgct ggcgttgtga atcgaaaaaa atatggcttg caagagtctg
     6421 tgaagttcat gagtaaaatc tataagaaaa attttgttag atataatcta tattgctggt
     6481 cagcaacttt gttgttggcc ggcaatatta ctacgatctg gctttgttgg cagcaaagcg
     6541 caccattgtt ttatttatta agtacattat tatcggtaat tacactgttc ctagtctttg
     6601 caatgttgaa ttgggtcgtg atcaaaagtc ttgggttgtg gatgatggga gaccgactga
     6661 ttgactattt gcttttcaca gcaacgatgt tgccaacggt agtcatgcaa ggcttggtgc
     6721 ttggaattta tttccgaata ttgatgattt tgcctgtgat ttttctgttt ggcggaacag
     6781 ctgtaacggt aatattgatt agtaaattaa ctgaaagagc gttagcaaag ttgccttgtc
     6841 agatacaatc gcactaattt tttcacaaaa aggagtattt ctttctatga agttaactga
     6901 taaacaagta gctaatatag cctttatcca gcaacttgag attccattga cacaaaaaga
     6961 tgaagaacaa atagacttga tttttactga cacaaaggct aatgtgcgac aaatcaagat
     7021 tactaaaaat gagcagcaac gaatttgctt gtcttattca catgaagact tgctgtatcg
     7081 aggattgttt atctgccttc cacgtttttt agctcaagga ggagagatgc catacattga
     7141 agaaacctcg atcattgaga ccagttattc attagatgct tctcgtaatg gcgtgatgaa
     7201 taaagagact ctaaaaaaat tcatttctta cttagcgttg atggggtaca acacattgta
     7261 tatttacaca gaagatacct atgaagtaga agaccttcca tattttggtt accttcgcgg
     7321 agcgtataca aaagaagaag tgaatgaaat tgttgaattt gcttctggtt ttggcataga
     7381 agtagttcca tcgatccaga cccttgctca cttgacccaa tttttgaagt ggtatgctca
     7441 acaagaactg atggatgatc aaaatactct actgatcggt gaagaaaagg tttatgcggc
     7501 aatcgaaaag atgattattg cctgcaaaga aatgtatcag acagagcgta ttcatttggg
     7561 aatggatgaa gcctatcaag caggtctggg acgatattta agcctgtatg ggtattgtga
     7621 tcggacaact ttaatgattc aacatatgga ccgtgtgtta tcaattgttg ataaatataa
     7681 gatgaaacca atgatttgga gtgatttcat ttataagcta ttagatgaaa ccggtaaaga
     7741 taggtattac aatcctgatt ttaaactcaa agcattagca gctaaaggat taccagcagg
     7801 attaacctat gttcattggg actatggttc agatgaagtt tcctcctatg agaaagtaat
     7861 tgataaccat ttgaaatttt gtgacattga tcattacgtt ttagctagtg gtgcccatat
     7921 ctgggggaga ctagccccga atcatgggaa aacagaagca attgtttctg cagctgtaaa
     7981 agcttctaaa aataaaggtg tacgttctgt gatgttaact acttggggag atgaagggca
     8041 ggaaacagag cattggcatt ccttaattgg ggcattaaaa ttagcagatt atgtctattc
     8101 tgaagaagag tggaatgatt ttgaggaaat ctacgatcgt ttattaggaa caggtagtta
     8161 tcaattaatg tccgaattac gatatttcga tgaagtggat accattacaa ataacaactt
     8221 acaaatggct tccgtgagta aactgctact ttggcaagat ccgttagttg gtatatatga
     8281 ctttcatgta gccgctcata acaaaattgc cactagaaca attggtactt attatcatga
     8341 gttagctcag aaattagata tgaataaaaa ttggaatcca aaacaaccaa ttttaacaac
     8401 tattcgtcaa cgatatatac ttttagctga aattttagca caaaaggcag aactaggcat
     8461 ctatttacaa cactgctgtc tggtagaaga ccttgcagga ttaatggaat tagcgaatcg
     8521 gtgtgaagca ctagccaatc gagtaaatga attggcatct attcatgaaa taatttggta
     8581 tcaaacatac aaaacctatg gttgggaagt attagaacaa cgatatgctg cattagctca
     8641 tcgtctgaaa accgttgcgc gtcgtattaa aaatcatctt caggggcaag atgccctaga
     8701 ggagcttttg gaagaacgat taccgttttg tcaatatcaa gtgccgcttg aaatttctgg
     8761 ctttaattat cggcaaagct cggtgtcagg ctacaattaa cgaaggaatc ttcccgttga
     8821 acggaccact tgctcatcta atgggaaagt ggttcgtttt ctattcataa gtagcttacc
     8881 actaactctt ttattaagaa gagaaaaaaa gtaggaagtg agaaaatatg ttatcggact
     8941 attatcaaaa tctgtcagtt ataagtgtca atacagaacc attacgtaat tattatattc
     9001 cttatgcaag taaagaagaa gcaaaacaag agttagaaag ggaaaaaacg agccagttta
     9061 tcgatctcaa tggtacatgg gattttcagt attatccttc tgtccaacag ttaccggaaa
     9121 atttttttca agaggccttt actgaaagct atgatagaat ttctgttcct ggagtttggc
     9181 aaacagaggg atttgactta cctcagtata tcaatgtatc ttatccaatt ccttttgatc
     9241 cgccttatgt acctaatgat accccttgtg gtgtatatcg gcgtcagttt caaattgagc
     9301 aaacagatgc acgcttttac ttaaatctag aaggagcaga tagtaattgt tttgtttgga
     9361 taaatagaaa ctttgtaggc tatgaccaag tgagtcatag tacatctgaa tttgatgtga
     9421 ccaagtattt acaggttggg gttaatgaaa ttcaaattct agtgatgaag tggtgtgatg
     9481 gtacctactt tgaagatcag gataagtggc gaatgagcgg tctatttcgt ccagtgtatc
     9541 ttcttcaacg accaactatg catctgacaa gttatcaact acggacgttg actgatccta
     9601 aaaaaggaag cggaacatta atggtttctt ttcaacgatt tactcctatt gaagtatcgt
     9661 tacctatttc ttacgaatta ttccatcctg atggacgtct catagcgaaa ggggagtgtt
     9721 actcggaaca acaaataaat ttggatgttg aagattgcct tttttggaca gcagaaacgc
     9781 ctaaattata tacattactt ttaacatgta atggtgaata ttttaaagaa aaagttggct
     9841 ttcgagcact gacgatcaag aatcgtaagt tattgctgaa cggaacacct attaaattat
     9901 atggagtcaa ctatcatgac gtgaatcagc aaggcacgcc agtgatgacc cgagagcagt
     9961 tacgtgcaga tttgttatta atgaaaaaat taaatgtgaa tactgtacga acggcacatt
    10021 accctaaatc accagatttt tatgaattgg cggatgagat tggtctgtat gtaatcaatg
    10081 aagcagattt ggagtgtcat ggtgtggtta atctatatga tactgaagcg aattatcaac
    10141 taattgctaa tgatccgaca ttcgaaaagc cgattatgga ccgagtgatc cgttctattc
    10201 ttccttatca aaatcgaagc tctatcatta tgtggtctat ggggaatgaa tctggttatg
    10261 gagtaaattt tgaaaaagct caagctttag cacggaaatt ggatgattca cggttgattc
    10321 ataatgaacg ctcgattgaa ccagttattg accaagtgaa tgattttagt aacttggatg
    10381 ttattagtca aatgtatccc ccactggaag atgttcatcg tcattgtcaa agaagtgata
    10441 ctgaaaaacc tttttttatg tgtgaatata gccacgctat gggcaacggt cctggagatc
    10501 ttaaaacgta cttcgatttg attcaacagt atgattcctt tcttggcggt tgtgtctggg
    10561 agtggactga tcatgcaatc aatagaggaa ctttagaaac gccatcttat ttatacggtg
    10621 gtgattttgg tgaaaaatat cacgacggca atttttgcgt agatggttta ttgaccccag
    10681 atcggcaatt aacagtaggt gcattggagt accgtaatat tcattcgcct attcaactga
    10741 ttgctgtaga tgaggagctg caagaagtaa ccttgagcaa tataactgct tttacagccg
    10801 gttcagcatt taacctttct cttgaatggt atatagatgg aaaaaaaaca gtttatgaag
    10861 taatgaatcc tttcatactt gagcctggca aaagttgttc attgcccatc acagtaccta
    10921 caacgattca tggtgctcat acggttgctg tttcgctatc tgtttataaa aatgatgagc
    10981 tttatggaca gcaacagctc tttcttaaaa aagaaaaaat gagattgcca agaactgctc
    11041 atcataatga tgcaccacaa atttcagtaa aaggcacaga gctgataatc aaaggagaat
    11101 cctttattta tatatatcaa ttagcaaaag ctgcgttcac ttctgtaaag atcaatcaaa
    11161 aggaactttt ggaacgtcct actcaatttt caatttggcg tgcaccaacg gataatgatc
    11221 gtaacttagt aaacaaatgg aaagcagctg gatatgctga aatgtcttta gaaatcagaa
    11281 aaaaagactg gaaattcgta gatgatcatc tcctgatcga aacgtcttat cgaggagtta
    11341 ttccaggatt acagccaatt tttacaatgc acatacagtt gaaaataaat ggtgcaggcg
    11401 taatcaacca aaaagtatca gtggagaaaa atccgatttt tccagatttc cctcgctttg
    11461 ggatggactg gccgttgaat gatacttttt cgaaggtttc ttattgtggc tatgggccgt
    11521 atgaaagtta tcctgataag cactatgctg cgatgtttgg tatttatgag atggagattg
    11581 aggaatttga tcagccatat atcaaaccac aagaatatgg taaccgctgg caatgtgatt
    11641 gggtatcaat caaaaatcat caaggatcta ccatgacggt ttatggaaac tctaattttt
    11701 ctgctctgcc ttatagtcaa gctcaattaa ccgaaaagtc acaccattat gaattaataa
    11761 aagaaaatag aacttggttc aatttaagtt ataaacaaaa tggtatcggc tcacacagtt
    11821 gcggtcctta tttagaagat cgtttccgtt ttatggaaga tacttttgac tggaatatag
    11881 tgattacttt caaaggagaa aatagatgaa agaaacaaag atcaatcaag tcaaaaaaaa
    11941 attatctcgt tatcaagaag ttgtttatcg aaaccattgc ttaaaatggt caaaaaataa
    12001 gctgaatgtt cgttttaatg gattatttta tcgtttcgaa cagttagaag aagtctggaa
    12061 accggtaacg aatttccaat catgtcctgt acagctcata aatgatccaa aagaaacata
    12121 cgatttatgt ataacacacc tttataacca gcatttgaaa aatgacggat tttatttaaa
    12181 ggttactaat gacaatcaac ttacgattga atcagcaaac cttcgagggt ttcaatatgc
    12241 tatggaagcc ttattagaga gttttttttc ggtaggagat caattattat taccggaatt
    12301 gattctgaaa catgaaccat cagtgaaaat tcgaggaatc attgaagggt tttatggtat
    12361 cccctggaca catgaaatgc gcctctctct tttttcatat atgaaagata accaattgaa
    12421 tacttttatg tatgccccta aagatgatga attattacgt aaaaaatggc gtgaactcta
    12481 cgatgcgcaa gaattagaca aatttaagga gcttttatct gctgctgagg ctagcaatat
    12541 cgatttttgg tacttaatta gtcctggaaa tgatattgat attacgtgtg aagaagacat
    12601 tcaagttctt ctgaaaaagt tagaacaact gattgagctt ggcgtatttc aatttggtct
    12661 attaatggat gatattgact atcaattaaa aggtgctgct aaacgccgtt ttcgagagcc
    12721 agcattcgct catgcctatt tagtgaatcg agtagaggaa tatcttagca ctcggttgtc
    12781 caatcatgaa ttagtcattt gtcctacaga atatgataat cgtcatggtt ctcgctattt
    12841 agcaacgtta agtcaagaaa taccagaaca attaccgttc ttttggactg gcccaagcac
    12901 gttagcggcg tcaatttcta cagaagagct gcaggaaatg gcttctgtct atcaacggcc
    12961 gatgctgatt tgggataata ttccggtaaa tgattattta gaggataaag aactattgtt
    13021 catgagtcca tatgagaatc gaacaccgaa tctttctaag gagagatacc aggttacagg
    13081 agtagtttcg aatccaatgg ctcaattaga agcttcaaag tttacgatca atagtatggc
    13141 taattactta tggaactgtg agcgttttga tcctttggaa acttggacgt cagtcgttga
    13201 aaaagtagtt ggaagtaaac tacaaccagc ttatcttacc ttaaccaatg cttttccaaa
    13261 tcattacaca agcagtctgc tttcagatga agaattactg ctagccaaag acccagaatg
    13321 ggtcacaaaa gaaatggctg ctttagtcca agcagtaaaa tggatccagc aagaacaaca
    13381 tacatctaga ttgagaacag aattggagcc ttggttacaa gcaatcacag aaagttggac
    13441 tttctggcaa gaatggcaat taaaaaaaga tccaaaagaa gcagaagaat ggttggctaa
    13501 gaaaaaaaca caccgcatag gacgtgattt agtaactgcc catttagagc aaattatcaa
    13561 aagttagata tcttaaaaaa taaaaagcaa gaaaaaggag aacaaaatgg tcaagagatt
    13621 aattagtgca aactacagtg atgtcaagaa aatgacgtca aaagagttga agcaatccat
    13681 caaagcgagt gaaggacggg tgattgtttg tgaaacggtt gctaatactc ttccacaatc
    13741 tggagaaatt acgaatgcag aagttgcgtc agcatttggt gcagatttga ttcttttaaa
    13801 tggtttcgat tgttatcgtc ctgcagtttt tggactacca aatgtgacga ttgagaaggt
    13861 ttttgatcta cagcaaacga ttgaaaaccc gattcctaaa ttaaaagaac tagtcggtcg
    13921 tcctgtggga attaatttag aacccattgc ccctaaaaca aatatgatga gtgagaaagt
    13981 agaaatcagc caaggaagag caagctctgt tgaaacgata caaaaagcag aagaactagg
    14041 agtcgatttt atttgcttta ccggtaatcc aggcactgga gtaaccaatt tagaaattac
    14101 taaatccgta aaaatggcaa aaaagtattt ttcgggattg attattgctg ggaaaatgca
    14161 tagcgccggc tcagaagagc cagttgtgaa taaagaggcg attatttctt atgcggaagc
    14221 aggcgcagat attttactag ttccttcagt agggtctatc ccaggctttt tagagcaaga
    14281 cctaattgaa gcggttcggt tggcaaaaaa atatgatttg ttaacgatgt ctgcaatcgg
    14341 tactagtcag gaaagtgccc gaccagaaac attgcggcaa ttagccatta tgaataaaag
    14401 ttgtggtgtt gatttacaac atattggaga tgctggatac gggggattgg caccagcaga
    14461 aaatattttc gaaacctcgg ttgcaattag aggaatgcga cataccatca actgcgttag
    14521 ccgctcgatc aatcgctaac aaaattaaat aaaaaatggt taaattgatt atttaagtgg
    14581 gaacatactt gagatatcag ctaacttctg tcactttaaa aaagggactg tgacagaagt
    14641 gaaaatagac aaaaaatatc cgaacaagag actaggatag cagaaaaact atcgttttgc
    14701 aacgaggacc aactggaccg taggagcaat tctcttattg ttcggatatt tcgcttgttc
    14761 aaggacttat gtctgtagtg cccttttttg gtaggaaaaa tggtagggaa ccatatcatt
    14821 tttaatgaaa atcaagcatt tcaaaagt
//
LOCUS       DACDSR010000063        14798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDSR010000063 DACDSR010000000
VERSION     DACDSR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.063"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            158..457
                     /locus_tag="IWK15_001946"
     CDS             158..457
                     /locus_tag="IWK15_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9547523.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            952..3117
                     /gene="acm"
                     /locus_tag="IWK15_001947"
     CDS             952..3117
                     /gene="acm"
                     /locus_tag="IWK15_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9547524.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(3389..3874)
                     /locus_tag="IWK15_001948"
     CDS             complement(3389..3874)
                     /locus_tag="IWK15_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9547525.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            4354..4995
                     /locus_tag="IWK15_001949"
     CDS             4354..4995
                     /locus_tag="IWK15_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9547526.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            5014..5649
                     /locus_tag="IWK15_001950"
     CDS             5014..5649
                     /locus_tag="IWK15_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9547527.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5667..6482
                     /locus_tag="IWK15_001951"
     CDS             5667..6482
                     /locus_tag="IWK15_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9547528.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6801..8237
                     /locus_tag="IWK15_001952"
     CDS             6801..8237
                     /locus_tag="IWK15_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9547529.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            8426..10873
                     /locus_tag="IWK15_001953"
     CDS             8426..10873
                     /locus_tag="IWK15_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9547530.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            11103..12170
                     /gene="rlmN"
                     /locus_tag="IWK15_001954"
     CDS             11103..12170
                     /gene="rlmN"
                     /locus_tag="IWK15_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9547531.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(12363..13466)
                     /locus_tag="IWK15_001955"
     CDS             complement(12363..13466)
                     /locus_tag="IWK15_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9547532.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            14019..14567
                     /gene="aac(6')"
                     /locus_tag="IWK15_001956"
     CDS             14019..14567
                     /gene="aac(6')"
                     /locus_tag="IWK15_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9547533.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         5113 a         2396 c         3099 g         4190 t
ORIGIN      
        1 gatttaattt agaatgtttt taaaataaaa atcgttgcca taacagtgta ttgaaaggtt
       61 ttaacaatcc agtaatgatt taaaaaaatc tagatgtagc gtatactttg tttatagaat
      121 gtagtgggaa gaaatatatt tttgggagga aatcgtgatg tctaaaagtg aagaaataga
      181 aaacatggta ctaaaattat atgatctcgt tttaaatcca gagatcaaag aaaaggaacg
      241 tatacttttg atagacgcaa agacaggctt agaaaaaggt cagtattatc ctaaagtcat
      301 aaataatttg gaacgtagtt tacgtccgtt agcaatcagg ggagaattgt ctaaaccagt
      361 atcaccgttt tatatggaga tatcaacaat aggaaaattt gaaaaagaat taggtcgagg
      421 aatggcttcg gcaccgatta cttttgggca tttataaaaa cgagttattc atctcaatga
      481 attaaaaagt atagtcaatc tcataatgca ggctaggttc atttatttga acagagtctg
      541 tattttttta ttctaaataa agaaacagat gtttctttat attgtatttt atcctttaat
      601 taacacaaaa acagctagta taaactaata atgttaatta aaagtgtatt ttatgaaact
      661 atcgttattt attaatgttg catttttgta agttaaaaat tggaaatata gattgaaaaa
      721 atgaaagcaa taagtaaaat aaaatgaata tatgcgaaaa aataatttac attaggaaaa
      781 accttgtaaa aacagttttt tggcatattt attttttata aatatttgta aaataactat
      841 caatttttta gttaaaagat tggaaatgag tgggggagga attgaagaaa ggatttttga
      901 gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa aatgaaaaag
      961 tgtagaaata ttattttatc aatgttattt attattacta atataacatc actgattcct
     1021 gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg
     1081 gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa
     1141 aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt
     1201 cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact
     1261 ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac
     1321 tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa
     1381 gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca
     1441 aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct
     1501 gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa
     1561 caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt
     1621 gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa
     1681 caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt
     1741 gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg
     1801 atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa
     1861 tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct
     1921 tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac
     1981 acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt
     2041 caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc
     2101 ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat
     2161 ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt
     2221 ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt
     2281 ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg
     2341 gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg
     2401 gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta
     2461 gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc
     2521 gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat
     2581 gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa
     2641 ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg
     2701 ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa
     2761 tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat
     2821 atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga
     2881 gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc
     2941 agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa
     3001 tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt
     3061 ttattgatag taactatctg tgctgggata ttcttacaaa aatataaaaa agtttagtta
     3121 gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc cgatttataa
     3181 tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata aatatggatt
     3241 tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt tgttacattg
     3301 tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa agacgcttga
     3361 attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc agatgttcta
     3421 agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg gtattgatat
     3481 ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg acttggattt
     3541 ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga acgcctagga
     3601 ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat tgttcagcta
     3661 gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt tgttttcctt
     3721 ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa gggacatttt
     3781 tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt ttttttagta
     3841 gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct ttttttatta
     3901 gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca cgtaaagtta
     3961 aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt ctctggttgg
     4021 tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct gaagcgtata
     4081 gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc agataaggct
     4141 ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc
     4201 ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga ggttttttct
     4261 gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg catttgccaa
     4321 gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga agttatgccg
     4381 caattattag taggagcact cacaacattg aaattatttt tatttacctt gattggctca
     4441 ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc gctacgctac
     4501 ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca actgattttt
     4561 attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc agcactcatt
     4621 gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg gatacaatcg
     4681 attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt tcaaacggtg
     4741 acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg gaatgaagtt
     4801 atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga cgtaatgcgt
     4861 gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg cgtggcaatc
     4921 atctatctta cattgactgg tattctaaca atcataatga aacaagttga aggaaaatta
     4981 aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa agtgaacaaa
     5041 gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga tgggaaaaca
     5101 ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat cttggctggc
     5161 ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa cccattcgag
     5221 cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct atttccacat
     5281 ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca gacaaaagaa
     5341 gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga tttattgaat
     5401 atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc cagagcattg
     5461 gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga tcctgaatta
     5521 cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac ccaaattatt
     5581 gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa agtttcccca
     5641 attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc gctttgatcg
     5701 caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat aattgggcga
     5761 ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt cctatgggat
     5821 ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa gcagtctttg
     5881 aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag gaatttgaac
     5941 tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg gcaagagaaa
     6001 agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc acacctaaag
     6061 aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg caaaatgggt
     6121 catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata gtcaaaaacc
     6181 aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa agcagacggg
     6241 tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa agaaacgaac
     6301 tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca gttggtgcac
     6361 gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa ctagagaaaa
     6421 caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg ccgagaaatt
     6481 aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa taaacagtgt
     6541 tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct cttggtaaac
     6601 ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     6661 ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac ctcctttaca
     6721 taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac gattgaaggt
     6781 tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca aaagattttc
     6841 tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa gatgggaaag
     6901 gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa ggaacgaacg
     6961 gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta atgaaagaac
     7021 aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct aatggacgag
     7081 gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa ttgattgaaa
     7141 acgatattga acctatgttg actttatatc attgggatct tccacaagca ttgcaagatg
     7201 aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat gcagaaacat
     7261 tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa caaaatatct
     7321 tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac ccgaaacgaa
     7381 tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat aaatttcacg
     7441 agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat tatccaattg
     7501 acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg gccaattatt
     7561 ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc ttagaagaaa
     7621 aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct gctaaaccag
     7681 attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca ttaaacggag
     7741 ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa actggagttc
     7801 ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg gactgggaga
     7861 ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac cgcatgccgg
     7921 tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat aaacaaatcc
     7981 atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa gaagcaattt
     8041 cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg cttagctggc
     8101 tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa acacaagaag
     8161 gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg attgaagaaa
     8221 atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga tatggccgtg
     8281 ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc tgaaaaatga
     8341 aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt tgtataatga
     8401 aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta aaaaaaggaa
     8461 aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga cgattggtgt
     8521 tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt ttttaggaat
     8581 attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc tctgtctttt
     8641 attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa ttggtaaaaa
     8701 ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt tacatgccca
     8761 tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat gggatttgtt
     8821 agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga tgattggagc
     8881 aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt tagcagcagt
     8941 gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc agctgttaaa
     9001 tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc ctgccaagca
     9061 agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag cgaaagaagc
     9121 aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg agaagaaaaa
     9181 agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga
     9241 gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa
     9301 tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga
     9361 tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa
     9421 tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag
     9481 tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt
     9541 cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt
     9601 ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct
     9661 tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg
     9721 tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca
     9781 accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt
     9841 acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg
     9901 gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga
     9961 agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc
    10021 tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt
    10081 agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc
    10141 tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt
    10201 accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga
    10261 agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat
    10321 cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc
    10381 ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa
    10441 tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa
    10501 accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt
    10561 tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga
    10621 aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt
    10681 cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa
    10741 ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg
    10801 aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga
    10861 taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca
    10921 aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat
    10981 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
    11041 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
    11101 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
    11161 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
    11221 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
    11281 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
    11341 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
    11401 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
    11461 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
    11521 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
    11581 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
    11641 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
    11701 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
    11761 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
    11821 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
    11881 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
    11941 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
    12001 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
    12061 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
    12121 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
    12181 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
    12241 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
    12301 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
    12361 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
    12421 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
    12481 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
    12541 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
    12601 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
    12661 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
    12721 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
    12781 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
    12841 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
    12901 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
    12961 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
    13021 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
    13081 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
    13141 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
    13201 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
    13261 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
    13321 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
    13381 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
    13441 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
    13501 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
    13561 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
    13621 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
    13681 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
    13741 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
    13801 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
    13861 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
    13921 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
    13981 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
    14041 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
    14101 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
    14161 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
    14221 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
    14281 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
    14341 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
    14401 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
    14461 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
    14521 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
    14581 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
    14641 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
    14701 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    14761 tttttgcata aatttggctt aataccgaga aactggct
//
LOCUS       DACDSR010000064        14552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDSR010000064 DACDSR010000000
VERSION     DACDSR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.064"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..543)
                     /locus_tag="IWK15_001957"
     CDS             complement(<1..543)
                     /locus_tag="IWK15_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9547534.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVD"
     gene            816..3287
                     /locus_tag="IWK15_001958"
     CDS             816..3287
                     /locus_tag="IWK15_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9547535.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3390..5126)
                     /locus_tag="IWK15_001959"
     CDS             complement(3390..5126)
                     /locus_tag="IWK15_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9547536.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5123..5827)
                     /locus_tag="IWK15_001960"
     CDS             complement(5123..5827)
                     /locus_tag="IWK15_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547537.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5958..6461
                     /locus_tag="IWK15_001961"
     CDS             5958..6461
                     /locus_tag="IWK15_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9547538.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6421..6759
                     /locus_tag="IWK15_001962"
     CDS             6421..6759
                     /locus_tag="IWK15_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9547539.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6780..8198
                     /gene="ffh"
                     /locus_tag="IWK15_001963"
     CDS             6780..8198
                     /gene="ffh"
                     /locus_tag="IWK15_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9547540.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8310..8888
                     /locus_tag="IWK15_001964"
     CDS             8310..8888
                     /locus_tag="IWK15_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9547541.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8892..9413
                     /locus_tag="IWK15_001965"
     CDS             8892..9413
                     /locus_tag="IWK15_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9547542.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9492..10046)
                     /locus_tag="IWK15_001966"
     CDS             complement(9492..10046)
                     /locus_tag="IWK15_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9547543.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10299..10574
                     /gene="rpsP"
                     /locus_tag="IWK15_001967"
     CDS             10299..10574
                     /gene="rpsP"
                     /locus_tag="IWK15_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9547544.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10586..10837
                     /locus_tag="IWK15_001968"
     CDS             10586..10837
                     /locus_tag="IWK15_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9547545.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10962..11753
                     /locus_tag="IWK15_001969"
     CDS             10962..11753
                     /locus_tag="IWK15_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547546.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11923..12444
                     /gene="rimM"
                     /locus_tag="IWK15_001970"
     CDS             11923..12444
                     /gene="rimM"
                     /locus_tag="IWK15_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9547547.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12434..13195
                     /gene="trmD"
                     /locus_tag="IWK15_001971"
     CDS             12434..13195
                     /gene="trmD"
                     /locus_tag="IWK15_001971"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9547548.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13331..13678
                     /gene="rplS"
                     /locus_tag="IWK15_001972"
     CDS             13331..13678
                     /gene="rplS"
                     /locus_tag="IWK15_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9547549.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13810..>14552)
                     /locus_tag="IWK15_001973"
     CDS             complement(13810..>14552)
                     /locus_tag="IWK15_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547550.1"
                     /translation="SNKYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDYKRESKTI
                     KIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNA
                     SVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQ
                     TTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGH
                     ENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         4754 a         2660 c         2952 g         4186 t
ORIGIN      
        1 atcaacacaa ataaaactca tggaaccaga aacagaattc attgatttga actcatccat
       61 acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg cttcacgcaa
      121 gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt tacgtgattg
      181 attttcgtac agttccatga gaatcacctg cttcagttcc tttgttagtt gatgatgttc
      241 atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt
      301 acgttttaat tctaaaatag ttaacttttc tttgactttc aacatctgag tgcgtgtcgt
      361 ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg aagaagagtc
      421 atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt caatcagcca
      481 ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag tatgattatc
      541 cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac caaaagtgag
      601 tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc gattttccac
      661 caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga aaggtgtttg
      721 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
      781 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
      841 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
      901 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
      961 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
     1021 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
     1081 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
     1141 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
     1201 gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
     1261 tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta
     1321 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt gatccattaa
     1381 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
     1441 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
     1501 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
     1561 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
     1621 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     1681 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     1741 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     1801 cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa ataatcagtc
     1861 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     1921 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     1981 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     2041 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     2101 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     2161 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     2221 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     2281 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     2341 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     2401 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga
     2461 taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga
     2521 aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac
     2581 aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc
     2641 gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct
     2701 ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa
     2761 tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa
     2821 acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg
     2881 gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa
     2941 ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc
     3001 tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta
     3061 gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag
     3121 ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt
     3181 tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta
     3241 gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc
     3301 aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa
     3361 ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc
     3421 gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct
     3481 tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg
     3541 cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct
     3601 tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt
     3661 tttgaatatt ggacagcatt ttcaattaga tttttgataa ttggataaag gatatctgtt
     3721 ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg
     3781 attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc
     3841 tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac
     3901 cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt
     3961 gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg
     4021 tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag
     4081 tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc
     4141 aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca
     4201 ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc
     4261 tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt
     4321 aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta
     4381 tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac
     4441 gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc
     4501 tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt
     4561 ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag
     4621 aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg
     4681 atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga
     4741 tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg
     4801 gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat
     4861 aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct
     4921 tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc
     4981 agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa
     5041 tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga
     5101 cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc
     5161 taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac
     5221 taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta
     5281 gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa
     5341 attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat
     5401 cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac
     5461 gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg
     5521 gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat
     5581 ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga
     5641 tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt
     5701 ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt
     5761 tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt
     5821 ttttcattca aatctccttt aaataggttt cattctgttt atattttagc atagaatcag
     5881 tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata
     5941 gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct
     6001 atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg
     6061 tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca
     6121 ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg
     6181 aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa
     6241 aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt
     6301 atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta
     6361 ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga
     6421 atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg
     6481 acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag
     6541 atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc
     6601 aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa
     6661 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
     6721 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
     6781 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
     6841 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
     6901 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
     6961 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
     7021 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
     7081 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
     7141 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
     7201 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
     7261 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
     7321 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
     7381 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
     7441 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
     7501 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
     7561 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
     7621 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
     7681 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
     7741 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
     7801 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
     7861 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
     7921 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
     7981 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
     8041 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
     8101 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
     8161 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
     8221 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
     8281 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
     8341 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
     8401 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
     8461 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
     8521 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
     8581 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
     8641 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
     8701 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
     8761 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
     8821 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
     8881 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
     8941 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
     9001 atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag
     9061 caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca
     9121 cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc
     9181 taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga
     9241 tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt acccctgtat
     9301 tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc
     9361 tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa
     9421 taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac
     9481 cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt
     9541 tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg
     9601 tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg
     9661 cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa
     9721 cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt
     9781 ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt
     9841 cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga
     9901 tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga
     9961 agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca
    10021 ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat
    10081 cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa
    10141 gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat
    10201 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
    10261 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
    10321 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
    10381 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
    10441 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
    10501 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
    10561 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
    10621 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
    10681 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
    10741 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
    10801 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
    10861 tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag
    10921 caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat
    10981 ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata
    11041 cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa
    11101 cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag
    11161 accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag
    11221 gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa
    11281 cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct
    11341 atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg
    11401 agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag
    11461 gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg
    11521 ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg
    11581 aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata
    11641 cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc
    11701 atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc
    11761 gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc
    11821 cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca
    11881 catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat
    11941 gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg
    12001 gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa
    12061 gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt
    12121 tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg
    12181 tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt
    12241 ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt
    12301 gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc
    12361 gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa
    12421 ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc
    12481 tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc
    12541 taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg
    12601 tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa
    12661 agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga
    12721 tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta
    12781 tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta
    12841 tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt
    12901 gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt
    12961 ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt
    13021 gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac
    13081 ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt
    13141 tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt
    13201 tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag
    13261 cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga
    13321 gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat
    13381 cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg
    13441 tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga
    13501 aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca
    13561 tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt
    13621 gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc
    13681 atcgttatca gactgagaaa gaacccttgg tattcaaggg ttcttttttt gttctctgtt
    13741 tttttatatt aacgatttct atttgggaca tttttgatta ataaaaaggg gcaaaaaagg
    13801 ggcaacaagt tacagttttt ctatatcttc tttcaatttt tcttttactc cctttgtcac
    13861 atgcaaataa atcttttcag tgatatcact attttcgtgc ccaacgcgat cctgaatagc
    13921 atacagagga gttcctagtt ccgctaattt tgaaatatgg gtatgtctaa aaatatgtga
    13981 actaagtttt ttatcaatct tcatgtcagc tttatgattt ctcaaatagg tgttaattgc
    14041 tgttagttgg aaaggagttc ctttcgttgt ttgaaataga aattgaccat tcggatttaa
    14101 ttctagaagc tcattgtaaa tagctatcgc tttttttggt aaatcaattt ctcgcattcc
    14161 agcagcagtt tttgtagaat cagatttttt catatcagct attgaacgtt ctctatacat
    14221 catcgtccca tttataacta ctgatgcatt attattggta atgtgtacat cgtctttact
    14281 taatgcgatt gcttctccag gcctcattcc agtcaaatat agccactgga aaaggagaga
    14341 atatctttta ttgtgtgaag ttgtgaaatt tactagtctt ttatattcat catcttctaa
    14401 aaacttatct ttaattttta tcgtttttga ctctcgttta taatcaataa ctacttcgtt
    14461 gataggattt ttttctacat agcctttttt catagcgtat gaaaaaagaa gattcaattt
    14521 ggatttaatt acactgacgt acttatttga ca
//
LOCUS       DACDSR010000065        14494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDSR010000065 DACDSR010000000
VERSION     DACDSR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.065"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..839)
                     /gene="truA"
                     /locus_tag="IWK15_001974"
     CDS             complement(99..839)
                     /gene="truA"
                     /locus_tag="IWK15_001974"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9547551.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1030..1665)
                     /locus_tag="IWK15_001975"
     CDS             complement(1030..1665)
                     /locus_tag="IWK15_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9547552.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1812..3053)
                     /gene="sstT"
                     /locus_tag="IWK15_001976"
     CDS             complement(1812..3053)
                     /gene="sstT"
                     /locus_tag="IWK15_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9547553.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3251..4249
                     /locus_tag="IWK15_001977"
     CDS             3251..4249
                     /locus_tag="IWK15_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547554.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4262..6280
                     /locus_tag="IWK15_001978"
     CDS             4262..6280
                     /locus_tag="IWK15_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9547555.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6340..7056)
                     /locus_tag="IWK15_001979"
     CDS             complement(6340..7056)
                     /locus_tag="IWK15_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9547556.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7283..7825)
                     /locus_tag="IWK15_001980"
     CDS             complement(7283..7825)
                     /locus_tag="IWK15_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9547557.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7830..8057)
                     /locus_tag="IWK15_001981"
     CDS             complement(7830..8057)
                     /locus_tag="IWK15_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9547558.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8136..10016)
                     /locus_tag="IWK15_001982"
     CDS             complement(8136..10016)
                     /locus_tag="IWK15_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9547559.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10205..10819)
                     /locus_tag="IWK15_001983"
     CDS             complement(10205..10819)
                     /locus_tag="IWK15_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547560.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10961..11521
                     /locus_tag="IWK15_001984"
     CDS             10961..11521
                     /locus_tag="IWK15_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9547561.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(11826..12140)
                     /locus_tag="IWK15_001985"
                     /pseudo
     CDS             complement(11826..12140)
                     /locus_tag="IWK15_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(12394..14029)
                     /locus_tag="IWK15_001986"
                     /pseudo
     CDS             complement(12394..14029)
                     /locus_tag="IWK15_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4580 a         2828 c         2301 g         4785 t
ORIGIN      
        1 aaaagtcttt ttttatattt ctttaatttg cttcatacat tatttaacat cgatatttat
       61 actgtgaaac atgcgtttca tccattatca aagtattttt atcgataata aacttcatcc
      121 aaaaacagac cttgtgcagg gacggtttct cctgcatgtt gccgcacttt gctttcaaat
      181 atttcatcga ttgtttctat cggcatggca cctgtgccga tttctagtaa tgtccccatg
      241 ataatccgca ccattttata taagaatccg tctccgacaa aagtaaaatg aagcatttct
      301 ccctttcgtt cgatagataa ttcatcaatc gtacgcactg tggatttttt tgattttttc
      361 aatgcagaaa aaccaatgaa atcatgtgta cccactaatt tttcgcaagc tgcattcagc
      421 ttgtccatat ccaactcttg agggaaataa aagctgtggt ttcgctcaaa tgctgaagga
      481 atcacgttgt tccaaacata atagctgtat tttttgccca ccgcattata tcttgaatgg
      541 aagcgctcag gcatttcttc gattcttttg atgacaatat cccgcggaag gtaacgattc
      601 ataaagtcga tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt tgctacttgt
      661 cctcgcgcat gggtgccagc atctgttctt cctgatccaa taatttctat ctcttcattt
      721 gtcatctgat gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc atctccaagt
      781 ctttgccatc ctgaatatct ttttccgtca tattcgattg tcaacttgat gtttctcata
      841 ttttagtggc tcctctgtct tccatttggt cttctctcta cctttttaag attgtagcac
      901 aaaatatagc tccttgcttc tctaagacag aagctataca agaaaactgt ggaaatatat
      961 atttcagaaa aacaaaaaac agccaccgga tgaagtatcc gctgactgtt caatttgacg
     1021 gctcaagttt tactgtttga gatgcaagat actcaacctg gcaatgggca cataattaaa
     1081 gaaaaaactg attctttggc aaaaacctat tttattcagt aatggccaag aatttataga
     1141 aggaattctt gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag acagtccaag
     1201 gtataagtgg cttacgtcca tttgcccatt ttttcgatag atcaagaaaa gaagaactgg
     1261 aaacaataaa aaaccactgt aacctaaacc tgatccgata ttatggaccc atgtcgagac
     1321 attccacgaa gattcatggg tattgatact gaacaaacct gtaaagatac aatcacctat
     1381 accatagaac gcgatggctg ctgtaactag ggtagctaac acacgagatg tactaacaaa
     1441 tgcagcatac aatgcaggca atgacaggat gaaaaagatc cctgaaacca cagaccaaat
     1501 aagaaacgct tttcttacag gactggaaac ctctccaaat acactgatca cgctcttcat
     1561 ttgatctaat tcaggataat acaatcctaa tacgtacgga gtcaaaaaat cactcagcac
     1621 gcctaataat aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac gcctccaata
     1681 acaattacct atgcttttaa ttgttattat aaacgacttt atcttgattt aacagaaacc
     1741 tctatttaca cgaacatcta tataactcat taaaaactcg aatcatttta aaaatactaa
     1801 atatctaacg ttcaacgttc acttaatcca tctaaacaat ctttcaattt gattttttcc
     1861 ccacttcttt tacgcgcgcc tagctctcct attgcagtaa acaacagatc acttgacgaa
     1921 tttaaggcag tttccattga atcctggata acaccgataa taaatccaat accgacgatt
     1981 tgcatcgcga catcattaga gataccaaac aaactacatg ctaatggaat caataacagt
     2041 gaaccgcccg caacacctga agctccacaa gcagctaaag cagacaaaat acataaaagg
     2101 actttcaaaa caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa cgtcatcaag
     2161 gaaatggtaa tcgcggcacc gcccatattg atcgtagcac ctaacgggat cgatatggaa
     2221 taagactctt ttgtcaattt caatctttca gccaacatca tgtttatcgg gatatttgcg
     2281 gcagaacttc gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa gaaaatcaat
     2341 ggatagggat tttctttaat aaacaaaaac gtgaggaatg ggtacacaac taatgcgaca
     2401 aacagcatcg taccgatcaa aacaagtaaa agctgcagat actctgccaa tccagcgatt
     2461 ccagtcgtag caattgattg ataaaccaat cctaaaatcc ctatcggagc aaatgcaata
     2521 atcacttgta cgacttgtga caacgctata gatatctgat ccaccaactc ttttgtcgat
     2581 tctgaacgtg ctcgaaaagc tagtcccaac aatacagccc agaataagac gccaaggtaa
     2641 ttcgcatcaa taagagattg aacaggattt tgtgtgacat tcgtcaacat agtagataaa
     2701 acactttcca aactttgcgg tgctttttca gatactgttt cttgtaaagt aataggaaca
     2761 gtaaaaattg aacttacaat aactgctaca agtgcagaaa gcaatgttcc cactaaatat
     2821 aagatcaaaa taggcttcac gaatgtctta ttcccaattt tatgcttggc aattgacgcc
     2881 atcgtcaaga aaaaaaccag aataggtgcg attgccttca agcaactgac aaacaacgtt
     2941 cccaatattg aaataaatgt ccatgtgggg aaaaagatac caaacaatat tccgactaaa
     3001 ataccaacaa caatacgttg aactaacgac atatccaaaa acttctctaa caaatgacca
     3061 ccctttcaca attttaacaa tcattgtatc attttatgca aagaaactaa aggtataatt
     3121 ttctaaaaat tctaacaata caaacaacca tttcttgcta attttgtact ttttctgtct
     3181 acacgttgtt ttttgagata ttctaagtta ggatgttatt aatgggcaac ttatcaggaa
     3241 acgttttctt atgtttttgt ccaatttttt tgtgaggtgt ttattcttga aaaaaataaa
     3301 gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg ttttacagcg
     3361 tatattgatt tcagcagtca ctattcctac atatgcaaca aaccctggtc gattctacat
     3421 ctatacattt ttacaaactt ccgttgttat tggtgcaaat ttgatcccac tttacatggg
     3481 ttaccaatat caaaaaatcg cccgtttaaa agttttacat tatttaagtc gttttgcctt
     3541 tatattccta atagccacac tagtttgcaa tatctttttt tacgtacaag ctggcagctt
     3601 gaatattcgt gattattggc ttatcctaac acctatcagt cagaattact ttacttatgc
     3661 tgtttcttgt actttgcttt tttgtagtat tccttataca gttggatttt taaataaatt
     3721 atcgaaaaca acaattaaat atctactatt atttttaaca attggattag ttggctgctc
     3781 tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt tatggatttt
     3841 ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa aggtccgcta
     3901 taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta ttttgatgac
     3961 acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt ctaaaccata
     4021 ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa tgctatctga
     4081 taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg tcactcaagt
     4141 tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac gtgaatttcc
     4201 aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat atttattagc
     4261 agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca ttcatcgttt
     4321 catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga aaaaaagaat
     4381 tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt actgctttac
     4441 cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta tccaggttgc
     4501 cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca ttattatggg
     4561 atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga ctgctacctt
     4621 gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc gggaagagcc
     4681 tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat cgatggttag
     4741 cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta ctagtattat
     4801 tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac gtcgatttat
     4861 cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc acaaaaattc
     4921 gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta accagcaagg
     4981 cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca ttaaaatcat
     5041 ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa agtacaacac
     5101 aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga cgatcgttct
     5161 caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag tacctacaga
     5221 ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga tgttatccgt
     5281 cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct tgagtatcag
     5341 taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc agaaaataag
     5401 tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta cagctgctct
     5461 ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt atttagacag
     5521 tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat cggatgattc
     5581 agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac aattcatcca
     5641 attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat tgaactacac
     5701 tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt atatgcaagg
     5761 ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca aaatcgataa
     5821 accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg ggaatgattc
     5881 gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca acaagtatag
     5941 tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact ttggtgctat
     6001 ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga cagaagtatc
     6061 taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc gttttaatgg
     6121 aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt taactgagaa
     6181 acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg caggtaacca
     6241 gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact gagcaagggg
     6301 ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt gtccataaac
     6361 gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt aaagataatt
     6421 atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt gctgaaaccc
     6481 gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat agacggctgt
     6541 gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa caggcactcc
     6601 accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa gaccatataa
     6661 gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt ggccccattt
     6721 ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt gttcaacttg
     6781 ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag tagatgtttg
     6841 agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg tttctgtact
     6901 tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg ttgctttcgc
     6961 tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag ctaacaaaaa
     7021 agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt tcgattactc
     7081 ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt attttctcat
     7141 caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg taatacatca
     7201 gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg ctttatattt
     7261 gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa tcttgtcgga
     7321 cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa cttaaaagtt
     7381 caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg attgcccgtg
     7441 tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt gttgaggctg
     7501 gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact tcttctaaag
     7561 tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg aaccatctcg
     7621 cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat gtacccaaaa
     7681 catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct aatacatgcc
     7741 ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt gtttcttgaa
     7801 caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc tgattggact
     7861 tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc atctggatca
     7921 aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac cccaggctgt
     7981 tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt atctaattgc
     8041 ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt attctagatt
     8101 acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc tttgctttcg
     8161 tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact agcttcgtga
     8221 acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag taaaaataag
     8281 acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt cttagccaag
     8341 cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc actcgtttcg
     8401 atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc caaagaggga
     8461 gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg gtattttttc
     8521 acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat gcctaacata
     8581 tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa ctgttttgct
     8641 cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac atctgacaca
     8701 cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct gcctgtttgt
     8761 tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg agcggcaatc
     8821 aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat tccttgtccc
     8881 ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt ctcctgagca
     8941 taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg agccactaaa
     9001 gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc agtagttcct
     9061 ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa agtatccatt
     9121 gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc tgctacattc
     9181 gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac agtgattgct
     9241 aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac ggttggagta
     9301 taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc ttgtgcttct
     9361 tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt tgcttcaatc
     9421 gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact gattttgacg
     9481 acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc gtcaatgact
     9541 cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc ttcttccacg
     9601 tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc agtagatggg
     9661 gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt ttgttccaga
     9721 tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc tccgatcata
     9781 aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt gactttcact
     9841 gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat ggctagagca
     9901 atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat aatcaacaca
     9961 gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa tttcatcttt
    10021 ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca tcataaaaaa
    10081 ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata cactacaaaa
    10141 aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt ttctatcgaa
    10201 attttcattg aatgattttc ctgtacagca tacatcggag atctatttct ttatctaaga
    10261 tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca tagaattgat
    10321 acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt ttttcaaatt
    10381 ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca gctaaaaaaa
    10441 tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt tcatttgctg
    10501 tctcgtaagc tcctttactt tctttagcaa atatattttg taaaaaatcg aatatcttaa
    10561 catatatttg ctgtccaaaa gaacgatgtt tctttttttc tccttttatc tccgccagca
    10621 aaacacctgc caactcgtcc tctgcataca aagcaagaat ttgagttgct cgattcaact
    10681 ccatatacaa aaaatatctt ccgtaaagat tcagcagaag ttcattttcc atatatctct
    10741 caaagttcat tccttcaaca gcaaaataga tagctttttt gtaatctttc ttttctaagg
    10801 gtttaaaagt tagtttcatc atttcgcctc ctcaacttca atgattgtgt tatacttaca
    10861 ttataataga cacagtgtct actatctata atcaatcatc tataagtttg tcgattaagc
    10921 gacagctttg ctttgatatg tcggataagg agataggtaa atgaaaaaga cagatagacg
    10981 cgtcaaaaaa acagaaaaag ctttggctga aacactatcc acattattag taaataaaaa
    11041 gattcaagca atcactatac gcgaactaac agaaacggca gatgtacatc gttcgacttt
    11101 ttatacacat tataaggata tctatgatct atatgatcaa ttagaaagca acttttttcg
    11161 ggatctaaat gaaattctct cttatgatcc agcccatagc tatgaggaac tatacactcg
    11221 cttgattgac tatgtttacg caaatacttt tttatatcgc ttatttatcc tcaaccatgg
    11281 tgatatttcg tttcagaaga aagttaaaca agttattgaa gaaaactatc tgaacatatg
    11341 gctatatgaa gacaagaaaa cgattatcac agaagaaatg agattcttta ctacgtacca
    11401 cgtccaaggt tgtctatccg ttctcaatcg gtgggtagaa agcaattaca agtactccaa
    11461 aaaagaatta ttagaactct tacagaaact gaatgatcac atagagaaaa tcatgccttg
    11521 aataaaaaca tactaagctt agttaagaag aaccgtaacc ttctcgtata acaactgaat
    11581 cttatttagc caaattgttt actgaagatt ttttgagtaa aatttctata attcctactt
    11641 attgaaaagt agtttgtagt gctgcttctc cctctctaag tgatatatct tgttgtacca
    11701 atcaaattca cctcatcatt aataaattac ttttaggcta atttattaca acttataggt
    11761 tgaagctgta accaatttat agtcatatta tatttttatc atccagttta ttgaattctt
    11821 ctcgtttacc tcaattcatt ttcaagggga atcagctcat catctaacat tttacgccat
    11881 gctcgcttca tttcttgtgg gtatttaaat ggtttcgctt ctggatgttt ggctttatat
    11941 ccttcgtaat ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc
    12001 atatattgca gattagaagc atgtaatttt ttctcagaaa caaaccactc attggaaaag
    12061 acatgaatca aatcatcact tacttgtgta aaatagcgac gtttaatgac aaagatatct
    12121 tcattgctat ctagtgtacc ttcatttctt tgtgcttaaa ttcaaagata attttctcaa
    12181 taaagatttt tgattccagt aagattcctt tactcctttt acaacatagc gttgtccttt
    12241 gttttgaaac atcttcttcc cctctttgcc attaaaaata gcccaattct ctataaagaa
    12301 gattaagctt cattattata atataaagtt taaatttggt ttattactta ctttttctta
    12361 ttctctagta agctaatatt gaaaaaaatg taatcagatc attattttta tatgttgtaa
    12421 cttcctgata agaaaactaa ttaaaagaaa gaggtaacta cataaactaa ttaaaaaagc
    12481 taacttcata gttggaggat aaatggaaga aacttcaatg attgtttctc cttgtgttcc
    12541 ttcaatcgac actagtccta ttatcaatct ctcttttttg tccatttact tttgcgactg
    12601 ttccccaata atcaaataca ggaagcgtta ttttttgtcc tttagattca tgtactgtat
    12661 agactatttt cgaatccatc acttgataag gcatagtatg ccattccttg ccaattctta
    12721 cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca tagtcataaa
    12781 gtcctccggg aaggagtgtc gtgtaacgaa taagattttc ttgattcatt accaagtgat
    12841 ctctctgagt gacaacttta tgttcatagt ttgcttttgt tgcagcagat acagccaaca
    12901 gtaataaaac aattgccgtt atattaacta cttttctttg cttagctgat gtgctcttca
    12961 tgaaaacgaa cgataaaatc aacgagccaa acagtacttg aaagcctaag attcgccaag
    13021 gaaattgaat catttgaatg ggtgtatttt gtaataaaga ccatggaaaa aatttggtgg
    13081 ataagagcca agagactacc gcactaaggt aaatcacttt ttctttgcga ttaaatcctt
    13141 taaaaaagaa gagtggacag atcaaagaaa taataccaat gattcctacc gtaaaggttg
    13201 tcaggtcatt attcatcgca ccaatcaaac tatccgagac atttaatgct tggtgttgta
    13261 aggtcgtttt atctggctca ttaatcgatt gaaccatgat ttgtcggaac atcggatacc
    13321 aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt tgacaactcc acaatcaacc
    13381 ggatgctttc tttttttctt gtttcactta ctacaaagcc gcctaagact agtaagccaa
    13441 cggctattac actcataaaa gtggaaagaa catgtgtata aatgagtaag ctcatactga
    13501 ctgctaatgg aattgcacat ggctttttct cataaatcag tgcgtaagca taatacataa
    13561 ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc agaacgatga taaatatcaa
    13621 tcgtacggta cccataaaat gtatataaac agctaaatat aaaagccgca tcgattcttt
    13681 ttaaaaattt tagtccataa tgaaacatca gcatgatagt acaaacattc aatagccaaa
    13741 catataagat ataactaacg attaaattat tacttatcca ataaaaaatg actgccggaa
    13801 aaaaggtcaa gtagggataa aaataattca ctccgctccc ataatgatta aacgtcgtaa
    13861 aattgattgg accagaaaaa attgaagata accctttgac tctagcaaaa tggaaggcaa
    13921 tatcccccga tgtagaaata gtatgaacat ttctaaaata attgattgaa taaagtccag
    13981 caataattgc taggagaatg gacaatatta tttttttctt tacttgcatt acttaattat
    14041 atactccttt catggattgc tttatctagt ataacttaaa ttttaaataa atcgttattt
    14101 atttttactc tcacttaaaa aagcactaaa attataagag agggaggtaa tggtctgaac
    14161 accaagaatt gttccaacat cgaattgctt aactaatgag ttcaaagctt aattatgtgg
    14221 ctcttatatt taccgagcgt ggattgcgat ctctttagcg aaacaaacat ctttcataaa
    14281 aaaaacagcc atatccgctt tcttggatat gactatttac tactacttta ctatctttca
    14341 ttcttcttgt ctcacttatg ataaggcgct gcgtatcatg agtgaatgat attaggaaaa
    14401 cttcataaaa taaatctttt atacatttcc acttctagat ggtcattcat gttagaagaa
    14461 tcaaactatt tttttactgt cgtaataata ttaa
//
LOCUS       DACDSR010000066        14455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDSR010000066 DACDSR010000000
VERSION     DACDSR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.066"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(367..4020)
                     /gene="rpoC"
                     /locus_tag="IWK15_001987"
     CDS             complement(367..4020)
                     /gene="rpoC"
                     /locus_tag="IWK15_001987"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9547562.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(4099..7716)
                     /gene="rpoB"
                     /locus_tag="IWK15_001988"
     CDS             complement(4099..7716)
                     /gene="rpoB"
                     /locus_tag="IWK15_001988"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9547563.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8444..9412
                     /locus_tag="IWK15_001989"
     CDS             8444..9412
                     /locus_tag="IWK15_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9547564.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9430..10335
                     /locus_tag="IWK15_001990"
     CDS             9430..10335
                     /locus_tag="IWK15_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547565.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10332..11138
                     /locus_tag="IWK15_001991"
     CDS             10332..11138
                     /locus_tag="IWK15_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9547566.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11295..12263
                     /locus_tag="IWK15_001992"
     CDS             11295..12263
                     /locus_tag="IWK15_001992"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9547567.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12580..13104)
                     /locus_tag="IWK15_001993"
     CDS             complement(12580..13104)
                     /locus_tag="IWK15_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9547568.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13391..>14455
                     /gene="scm"
                     /locus_tag="IWK15_001994"
     CDS             13391..>14455
                     /gene="scm"
                     /locus_tag="IWK15_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9547569.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4106 a         3105 c         2828 g         4416 t
ORIGIN      
        1 ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac
       61 ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat
      121 agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa
      181 aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc
      241 ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa
      301 attattttag acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta
      361 aaatatctat tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc
      421 tgaaatactg tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata
      481 acgttgcata ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc
      541 aagaagagga tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg
      601 gaatgatgca gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa
      661 gacaggtcga gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt
      721 aaagtctgcg atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac
      781 acgaacttta cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac
      841 cccttgcata cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac
      901 atcgcgaact tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg
      961 gtggatcata tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg
     1021 tgtatctgtt tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat
     1081 atcgatgaca tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc
     1141 aaagatttct tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt
     1201 atggaatgta cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt
     1261 accaactgct tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg
     1321 tttgcagaca ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac
     1381 acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat
     1441 caattcattt gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc
     1501 ttctaatgat tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc
     1561 agtaccgcaa tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt
     1621 caagtaacct gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg
     1681 agttgagata aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa
     1741 ttccatgatt cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa
     1801 gttggaaatg ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc
     1861 caaaccttcc atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac
     1921 acgttcataa cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc
     1981 aacttgttta tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc
     2041 gatccctact gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc
     2101 aagcatctta gaagtttctg ttactttgaa tcgtttgaag acttctgcga tgatgtttcc
     2161 taagtttttc ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc
     2221 agtaccagct tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg
     2281 ttcattcaga taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt
     2341 tgtgatcatc gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg
     2401 gttcacaccg atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc
     2461 gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta
     2521 gtagttacct agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt
     2581 caagatgttt tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa
     2641 tggaacgtgg acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag
     2701 tggatgcaaa cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag
     2761 tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa
     2821 aacatcccat acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga
     2881 agctagttcg cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat
     2941 ttcttttgga agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg
     3001 acctgagtag tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt
     3061 caacatatga gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg
     3121 acgaccgtta tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac
     3181 gatgattcca ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac
     3241 acgacggtaa agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat
     3301 tgggcgtaaa tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt
     3361 tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg
     3421 tccttgtgca gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt
     3481 atcaagtaat tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg
     3541 ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt
     3601 tcctggttcg atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct
     3661 catgtctaat acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac
     3721 aggagctgct aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc
     3781 cactccgcat cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc
     3841 acattcccag tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cacgttcagg
     3901 ttttaaggta cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct
     3961 gattttttcg ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa
     4021 aaggggttcc ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct
     4081 tgcagctcat agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac
     4141 atcgtctttt tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg
     4201 ctgttcggca aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat
     4261 gtcgcgaagt tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga
     4321 ttgcaattct ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc
     4381 acccttgacg attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt
     4441 caagatttct tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc
     4501 accaaaacgt tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga
     4561 gtaaggtccg attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat
     4621 gtacatcaca ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag
     4681 aacagttttc gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc
     4741 acttgcacca tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc
     4801 caagtgtaat tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag
     4861 catgatatcg attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg
     4921 ggatacaacc cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg
     4981 tttttgaacg atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc
     5041 ttcacgagta aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa
     5101 tgatgtatcg cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc
     5161 tgcagataat tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg
     5221 gacttctgca ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc
     5281 gacattaggg atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga
     5341 ttcatattct tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat
     5401 gatgatcgca tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg
     5461 gcctagagcc atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc
     5521 gactttctca cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg
     5581 acggaatttt gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc
     5641 agaagcatca acgtattcaa cgacaccgtc atgtttacat aacaaagcag ctcctgagtc
     5701 atgcgctgat ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg
     5761 cactgcttga cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa
     5821 gaaaggaata catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc
     5881 tactttatca atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg
     5941 agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc
     6001 gatatcagct gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg
     6061 acgataaggt gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt
     6121 gatcaacccg atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga
     6181 gtagtgaacg tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa
     6241 ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg
     6301 tgacaactga gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat
     6361 caattgttga ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg
     6421 ttccatacgt gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat
     6481 acgacgattt cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa
     6541 gaaatagctc attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg
     6601 atagccgttt ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat
     6661 gacttggaca gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga
     6721 atttaggcca ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt
     6781 tcctttttca acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag
     6841 attcaataga cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc
     6901 taagtcgtaa cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc
     6961 tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct
     7021 tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc
     7081 gccgaagatt tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt
     7141 taaaggaatc ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc
     7201 catttctagc caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt
     7261 tttgtcgact tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac
     7321 tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa
     7381 gaatacttct tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg
     7441 taatggtgca gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc
     7501 tttcaattca taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa
     7561 aatgtcttca aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg
     7621 aatttcgatc aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc
     7681 gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca
     7741 ttcaaaaaga ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac
     7801 aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa
     7861 accttacttc gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt
     7921 tcgtttctgc cttttcttca tcagatagtt tttgcaactt catatactac acttattttc
     7981 tataaaagtc aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt
     8041 tcaattttat gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt
     8101 atactttaga aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg
     8161 caccaacaca actgaataac ttttacttta gctactttag ataggtttgt attcatcatg
     8221 atggatacaa acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca
     8281 gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt
     8341 atcaagcatt ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt
     8401 ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat
     8461 catcggatta ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc
     8521 cgcagaggca tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc
     8581 gttagatgct gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg
     8641 cgataatcta acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat
     8701 gagtgagaaa ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc
     8761 acaatcttta ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt
     8821 ggaacaagcc catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac
     8881 agatatggtg ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa
     8941 aacaatcggc atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc
     9001 agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa
     9061 tgatgtccaa caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac
     9121 tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac
     9181 acctgtcgtt gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat
     9241 cgactacgaa agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa
     9301 gaatccaagt gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga
     9361 catggccaaa gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg
     9421 aggattctca tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta
     9481 tgggccctac ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg
     9541 actgctgaag gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc
     9601 tatcatccat tgacagcttg tcttctaggc tttttagggg gtgcggcagc agggcttgtt
     9661 tccggactgc ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg
     9721 actggtttat attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt
     9781 cagccaacga tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg
     9841 atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca
     9901 gaaacaggac ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg
     9961 atcaacacag ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt
    10021 tctggctcct tacttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg
    10081 atcgtaattg gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta
    10141 tttctccgtc tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta
    10201 gtcttcgaac tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg
    10261 atctgtcttt ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc
    10321 ggtagtgtca aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag
    10381 gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact
    10441 tcgtgacgat catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg
    10501 gtacattttc tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat
    10561 ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag
    10621 ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg
    10681 gcttcttccg tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc
    10741 taaatttggg gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac
    10801 gtcaagctat tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg
    10861 aacacactgc agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc
    10921 tgatcactga gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc
    10981 gctatgggag tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag
    11041 aggaaaaaca gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca
    11101 cggcattaaa agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa
    11161 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
    11221 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
    11281 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
    11341 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
    11401 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
    11461 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
    11521 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
    11581 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
    11641 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
    11701 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
    11761 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
    11821 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
    11881 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
    11941 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
    12001 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
    12061 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
    12121 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
    12181 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
    12241 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
    12301 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
    12361 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
    12421 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
    12481 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
    12541 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
    12601 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
    12661 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
    12721 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
    12781 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
    12841 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
    12901 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
    12961 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
    13021 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
    13081 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
    13141 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
    13201 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
    13261 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
    13321 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
    13381 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
    13441 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
    13501 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
    13561 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
    13621 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
    13681 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
    13741 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
    13801 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
    13861 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
    13921 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
    13981 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
    14041 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
    14101 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
    14161 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
    14221 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
    14281 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
    14341 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
    14401 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DACDSR010000067        14309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDSR010000067 DACDSR010000000
VERSION     DACDSR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.067"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            53..811
                     /locus_tag="IWK15_001995"
     CDS             53..811
                     /locus_tag="IWK15_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9547570.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            871..1299
                     /locus_tag="IWK15_001996"
     CDS             871..1299
                     /locus_tag="IWK15_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547571.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            1345..1417
                     /locus_tag="IWK15_001997"
     tRNA            1345..1417
                     /locus_tag="IWK15_001997"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1378..1380,aa:Gln,seq:ctg)
     gene            complement(1567..1938)
                     /locus_tag="IWK15_001998"
     CDS             complement(1567..1938)
                     /locus_tag="IWK15_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9547572.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            2153..2338
                     /locus_tag="IWK15_001999"
     CDS             2153..2338
                     /locus_tag="IWK15_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547573.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(2421..2609)
                     /locus_tag="IWK15_002000"
     CDS             complement(2421..2609)
                     /locus_tag="IWK15_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547574.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2633..2896)
                     /locus_tag="IWK15_002001"
     CDS             complement(2633..2896)
                     /locus_tag="IWK15_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547575.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            3054..3311
                     /locus_tag="IWK15_002002"
     CDS             3054..3311
                     /locus_tag="IWK15_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9547576.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(3382..3573)
                     /locus_tag="IWK15_002003"
     CDS             complement(3382..3573)
                     /locus_tag="IWK15_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547577.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(3714..4034)
                     /locus_tag="IWK15_002004"
     CDS             complement(3714..4034)
                     /locus_tag="IWK15_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547578.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(4057..4491)
                     /locus_tag="IWK15_002005"
     CDS             complement(4057..4491)
                     /locus_tag="IWK15_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9547579.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            4826..5464
                     /locus_tag="IWK15_002006"
     CDS             4826..5464
                     /locus_tag="IWK15_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9547580.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(5788..5871)
                     /locus_tag="IWK15_002007"
     tRNA            complement(5788..5871)
                     /locus_tag="IWK15_002007"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5835..5837),aa:Leu,seq:cag)
     gene            6399..6899
                     /locus_tag="IWK15_002008"
     CDS             6399..6899
                     /locus_tag="IWK15_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547581.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(7089..7433)
                     /locus_tag="IWK15_002009"
     CDS             complement(7089..7433)
                     /locus_tag="IWK15_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9547582.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(7587..8507)
                     /locus_tag="IWK15_002010"
     CDS             complement(7587..8507)
                     /locus_tag="IWK15_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9547583.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            8694..9185
                     /locus_tag="IWK15_002011"
     CDS             8694..9185
                     /locus_tag="IWK15_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9547584.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(9209..11584)
                     /locus_tag="IWK15_002012"
     CDS             complement(9209..11584)
                     /locus_tag="IWK15_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9547585.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            11910..12536
                     /locus_tag="IWK15_002013"
     CDS             11910..12536
                     /locus_tag="IWK15_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9547586.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            12559..13263
                     /locus_tag="IWK15_002014"
     CDS             12559..13263
                     /locus_tag="IWK15_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9547587.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            13455..14288
                     /locus_tag="IWK15_002015"
     CDS             13455..14288
                     /locus_tag="IWK15_002015"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9547588.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         4336 a         2773 c         2437 g         4763 t
ORIGIN      
        1 taaaaataca tttataatag gaaagataaa cagctgaaaa gggagatttc aaatgaagaa
       61 aaaaacgttc aaaaaactat tgtttctagg gggcacagct gctcttttat taagcggtac
      121 aagttattac ctacatatcc attctccaga acatacaatg aacactgcac aaaaaagttc
      181 aacacaaaaa acgaaaaaga caaaagagaa agcagaagtt ttactagatg ttccattaga
      241 gagccagttt gattctcctt ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct
      301 tttatctttt tatggttatg aaacaactaa aaaccagtta gctgagcaat tggattacgt
      361 ccctgttttc aatgcagatg ggactcatgg cgatcctaat gaaggatttg ttggtgatat
      421 cagtggcgga gactgggcta tggtcgtcta tgttccccca gttgcctccc ttgctcagca
      481 aatcgtccaa acggattatc acacttttcc aaaaaccgat gctcaactcg atgatatcaa
      541 aaaagcacta tctaaaggaa atcctgtctg gacttctgtg actatcaatt ttgaagtccc
      601 agatgaaact gattttatga cttggaccac aaataatgga gaagtaagag tcactcctct
      661 tgttcatgct tgtgtagtca ctggatatga caaagaaaat atttatgtca acgatcccta
      721 tggtgtaaaa aatcgagcag taccgatcaa cgatttttcg gcgattttca ctgcaatggg
      781 tggacagatg ctaactttgg agaaaagcta gtaattcaaa caaaaatccc ttatactcaa
      841 gataccaaac ctttgaaggg agcgtagata atggaaaaat tcacacataa aaaaatggat
      901 ccaaatgaaa ttccgattat ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc
      961 tctattaatg agtggaacga gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa
     1021 ttgtatcgtc aatctcttgt ttattacgca gatcaagagt acgagaaagc caccgattta
     1081 ttgaaatttc taattgctcg aacagagtat actcgctttg aatatattga acgtttagca
     1141 aacatctatc atattatgaa tgaacctgtc aaagaatatc agttattgga caccgtcttg
     1201 tcagtagcag aactgattgc ccttcctgct ggattagaaa agaaactagt acgccgcctt
     1261 ttacgagtaa agcaacaact atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt
     1321 gctatgatta ttttgttgaa taaatgggat gtagccaaac tggtaaggca cttgactctg
     1381 aatcaagcaa ttgtaggttc gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat
     1441 gaaccccctg taaaagaaca atctttttgc agggggatta ttgtattatt tttactactt
     1501 gtatttagtt tacataatta aattatgaat aataaaatcg ggcgtattct taatacaaaa
     1561 actgatttac ccaataattg ctaatgcttt ctccggaact ttttctctag gaacttcctc
     1621 atacgtttct atgtgtgctc ctttattgct aattttcaaa taatgatttt cttttaacac
     1681 agaaagacca ttaaattcaa ccgttcgttc tttcccattg gcatcagcag ctttctgcac
     1741 ataccttgct gtaccatatt catttacttc ctctggtttt tgtgtcttta catagacctc
     1801 tcctttttca actaatggat ttagctgatc aagtatacca gggatttctc cataacttcc
     1861 ttcagtataa tatttcagtc caaatagcaa aataccacta atcgccgcca gtccaatcaa
     1921 atatttaatc actttcatct ttctacctcc ttgttatata ttcagtatat gggtcagtta
     1981 aagaaattcc atccgtccca agttggagat ttttctccta ctttagaagc gagaatcgag
     2041 tgttttttta gtaaaaatga tcgttatttg atattattga cgtgtggata aatcagtttt
     2101 tctcgttttg tccatgtaaa aaatcatgtt gatagcaaag gatgtgttca aaatgaatca
     2161 agaagaatta tttcaaaaag tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa
     2221 acgatttatc gaagaacata aagaacaatt aggaccttat aaagagaaag cccaaaatct
     2281 tttaaaagat gtaaatatcg acagtgtcaa aaacaaattt aaaaatctat tcaaataagt
     2341 aaaactttca cttttgtaaa atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt
     2401 tcttttctga atacttcata ctaaaataaa cgtgtcggat cgtaatgata agtcacattg
     2461 ccgttcagtg aggcttttcc caccacatat ttacgattct ccaacttcag ccacgcttct
     2521 cgaaaagcca ttaattcttc tctttttaca tcaatctttt cgatttttcc atcgatcaaa
     2581 tcttgaataa gttgttcata tggattcatt tttttcctcc atctgctgtc aatcaataag
     2641 ttgtaaaaat aggttcatat gcttttttgg tatctagatc ttctctgact ttgactgtcg
     2701 tataaataat tttttctact gggatactat aataacttcc ttcaccatta ttgaatcggt
     2761 gaacgtcttt ctttgctaaa ataccattta ttttattcgt cagtacacgt tgttcgacac
     2821 tgttacttac aacatagtaa atcacccgaa tattattgat caaatgcaaa tcgatttcta
     2881 acactacatg atccatgaaa atcccactcc ttctttcact ttgattatag caagtttctc
     2941 ctctaatttc aaaatcgata cagattttac cggataaatc cacaaaactg cttgcgccat
     3001 ttcgcacaaa gtgttatttt gtatcatgga ctttatttat cggaggcaat agtatgatcg
     3061 cgatcggtca atttgttttt tatattcctt tttttattat gataagcatt cttttctatt
     3121 acataaaatg gacaaaaaag aagttttcag ttttactggc ctctttacct gctgtatatt
     3181 ttacttatca aattttttct tttaggcatt gggaaacaac gtctgtatta ataacacaca
     3241 tcatcgaatt gacgctttca gtaatttttc ttattatttg gatctatttt ttatataaga
     3301 atcagaattg aatccctcgc atgtttaaaa agaaaacgat taaagatgca gcaaatttct
     3361 gccgatcttt aatcgttttt tctattctgt tataccaaaa aaatgtttga atcggtgaag
     3421 gatcgttcct tgttttaatt ctataaattc ttgtgcgatc gggtactttt cttccaaatt
     3481 ttctttgttc ttttcgatca ggcttttcgc ttcttcatat tttttatctg cacatagtgc
     3541 ttttacttcc tgtaatactt ttcctgcttc catttgcctc accctcttgc cattttattg
     3601 ctttaagttt tatttaagtt atacgcatta ttctagtttc atcccaaata accttttttc
     3661 atttttactc ctcgaactaa tcagttttct aaaggctgat tggttcgtct tttttatttt
     3721 tcctccattt cattctgctc ttctgttaat ttatctaatg ctttgattga tgctgcggct
     3781 cttgcataat catagctgta taattccata aatttatcag gcagaacaaa ccacgctgga
     3841 tgataaccgt tagctgtgac agggaatcct ttatcttgtt ggttatctcc atcccatctc
     3901 agagcaacac ctttttttcc tttccacttt actaaagcga tcgagtatcc cttctttgtt
     3961 tgtgtgccgt cttcaataat tgctaaaatt tctatttttt cttttggcgc acggacttct
     4021 tgaggacgaa ccattttcta ttcctccctt atctcattac ttcacgacca acacactgca
     4081 aggggcatgt gatacgacat aagaagcggt cgatccaact aatgcttgtt gtatagcacc
     4141 ttttcctgtc gctcccataa cgatcaaatc aatattttct tgtccaggga tgacattggc
     4201 aatcaaattt cttggatctc cagtttcaac tattgcgtgg atctcagcaa tgccgtactc
     4261 tttggcatca ttgatttttt tcagcatggc tacttctgtt ctttgttttt catctgcata
     4321 gatttttgaa aaggcatagg cactatgggt cagctcaact ttgttgatga ttgacacgat
     4381 aaataagtgg gcttcattgt cctttgctaa gtcaagggct tcagcgaacg ctttgatcga
     4441 ttgtcttgat ccgtcaactg ctaccatgat gttctggtat cttttactca ttggaaccgc
     4501 ctcctttttc ttttattgta acatctcaag caaaaataca aaaaatatac gcaatatgta
     4561 aataaaatga tgaacaaaag caatttcttt caagttattt atacatttct tttctcatca
     4621 gaacacctgt tctaatccca ttatttctgc attatgtgaa attcctcatc atttttcaca
     4681 tcgagcgaac atcattctgt tacaaaaaac gtggatttgt cataaattgt catgcttctt
     4741 ttacctatga aatattttca cgtgttaaag tattcctcgt tgaacacaac aaataaaact
     4801 ttttaacgaa taaaggagat ttttcatgac atcacttaaa actttacttt ttggaacaac
     4861 tttggctgcc ggcgctgcat tcttcatggg aacgactgct catgcggacg aagcttatac
     4921 cgttcaatca ggtgatactc tatcaacgat ttctcaaaaa tacgttggtg ataactcctt
     4981 gatcaatgcc attgcagaat caaattcaat ttctgatatt aacctgatct actcaggaca
     5041 acaattaaca atcccaacag aaggctctgc acaagcagct gctgaacctc aagcagccgt
     5101 tcaagaagca ccagtccaag ctgaacctgt tcaagctgaa caacctgttg tacaagaaac
     5161 ggttcaaacg gaaacacaag cagcgcctgt tgctgaaaca caacctgctc ctgctgtaac
     5221 agaaactgct gctactccag caagtacaag ctctgcaaaa gaatggatcg cacaaaaaga
     5281 atcaagcggt tcttacacag caacgaacgg acgttacatc ggccgttacc aattagattc
     5341 ttcttactta aatggtgact actctgctgc aaaccaagaa agagtagcag aacaatatgt
     5401 tacttcacgt tatggttctt gggaagcggc gaaagcattc tgggaagcaa acggttggta
     5461 ctaagattca ttatttgaac gtttaaacac attcaaaata tagtaagagg tcatgcgatt
     5521 tattctttcg catgacctct ttttgagttt aagtttagtc tttattcctt aactatcgtt
     5581 tgccagtatt tcttgtatca gaatctataa atctactatt tgaatcaatc tattaggttt
     5641 taaactgtcc caccttcaca caaatttttt ttgatttatt tgaatctggt tccgttgttt
     5701 aaaatttttc ctatattctt ttagataata tgttattttc tttctcatcg ctccaggtgt
     5761 accttctctt tataaaaaaa caaattatgc ggctaagaag actcgaactt ccacgaggtc
     5821 accctcacta gcgcctgaag ctagcgcgtc tgccaattcc gccatagccg catcacatct
     5881 tttagtttag gattacatgt gttttttgtc aagaacatat ttttgttctt tttcatttct
     5941 gtacgccctc ccttatgcca tctacacaaa taattcatcc gagcaactcc atcatctatt
     6001 gagggttggt gaaaaaatct gttttatcca tatgatcgat ttcaatgtta aacgaacgac
     6061 tctgggatta gtaactattt tatttttttc ttcatttctt ttttcctctt tttctcgatt
     6121 cagctatctt ctttcatttg gatattgatt atttcttctg ttgattacta tccttctgga
     6181 attctagacg ctgaagtaat tcacgttttc tttcttctag agatcataag taacgaatga
     6241 acgaacttca aataattttt ttattgtaat tcatatttgt attgttcgat gattcttaat
     6301 acgatatgac acaataaata aaaataggca tggctattta caacaatagt gctatagtag
     6361 aaaacaaatg aataaggtta cttaaagagg tgaatacgat gaaactgatc aaactatggg
     6421 aagcagattt aaagaaagct tatcagctac aaactagctt tggaccaaat gagagtggat
     6481 ttatcaacaa tgcttatggc tatacatttg ctgaattttt agattatgta aaaaaatgcg
     6541 aaaaaacgtc tttaggaatc gatcttcctg agagttacgt tcccgcaacc gtctttattt
     6601 tagaaagtga tgaacatgag tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa
     6661 cattaagaaa cggcgctggc catattggtt acggcatctc ccctaattat cgtaaaaaag
     6721 gatatgccac acaaggatta gcattagtac tacaagaggc aaaaaaaatc ggcattgatg
     6781 aggcactttt gtctgtaaat aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg
     6841 gggctgcgat ttatcgtgaa gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa
     6901 aaagaaggat tccaaaagga aagggatttg aatgtccttt atcaatagaa cacatttatt
     6961 taattaactt ataatatagc agacaaaatt ttataaacaa agaaaccttt tatctctttt
     7021 cttctagaaa ggataaaagg cttttttgtt tttcctattt gcttctcata gctagtcagc
     7081 caagatttct atcgtatccc caacgcaatc cgtgcataac ggctcatctt atctgtcgtc
     7141 catgctggat accagactag tttgacttct gctttcgtta cttctggcac ttcttttaat
     7201 gcgtcatgga tagattctgt taaaatatct gctaatggac agcccatagt tgttagagtc
     7261 atcttaatga ctgtttctcc attttcagga ttgaattcta tatcataaat caagcctaaa
     7321 ttgacgatgt cgattcctaa ttcaggatca atcaccattt ccaaggcagc aagaatacgt
     7381 tctttgatta gttctatttc ttgtccagac cgttcttggt ttgcttcgct catcgtacga
     7441 cactccttaa cacgattttt atttatcata ctcttttttt agctgcttgt aaatgagaat
     7501 ttagaagtaa gattctcgtt taaatttata aaaatgttct tattagatgt aatagattaa
     7561 atttattcta aaataaacct gattcctcat tttttttgtg aacgattttt tatttctggc
     7621 aatacgccag ttgttcgata cattttttgt tcgttttcat ttgcaaaccg gccaaatact
     7681 gaatgagttg gtaaaatatt gataaatgca tcgtcgtcga tttcatgaac gatctgatca
     7741 agatcataca tttcgtagcg ggtgatgacc atcatgatca tgttcccttg tttttttgaa
     7801 tagccaccga cagacggcag taatgtcatt ccacgtacca tccgttttgc tagttcttct
     7861 gttacctctt ctggcttgac ggtaacgatc attgctgtta ctttttgatg actcgtgtgg
     7921 atggcatcaa ttacttgagt cattgcataa atggaaataa tcgtatacaa ggcactttcc
     7981 cagttaaaga agaagcctgc gatgactaca atgataccat ttagaatcag catgtagttt
     8041 ccgactgttt ttccagttgt ttgtgaaagt acaagagaga tgatatccat cccaccagtt
     8101 gtaaagccca tttttaaaga taatcctacg ccaagaccta ataaaacacc gcctactagg
     8161 gcattcatca aaatattatc agtgatctgt ccgactggaa gcagcatcgt catcaccgat
     8221 acacatacaa cattcacaaa actaaggatc gttgctcgtt ttcctacttt gagaaaccct
     8281 aatacaaaca cagggatatt caaaagaaaa ataaatgttc ctgtgtctat cttgatatga
     8341 aaccattcat ataaaagcga ggaaatgatt tgagcgatcc cattcatccc tgcagaaaag
     8401 acattggctg gaatcaaaaa ataatttaaa gcgaccgcag cggtcaatcc ggttatcaaa
     8461 ataaccacta ttttctttaa tctttcattt ttatgatacg cagtcataat tttactcaca
     8521 agcacctctc ccctactttg gtattccaaa tgtaagaata acagtttatc tattaaaata
     8581 aaatacttcg aactcaaatg agaagaattt tagcaaacag gtaagaatct gccctaatcc
     8641 tatgataaga tagatgtatg gtaaatattt atatgtatat caggagggtt cctatggcaa
     8701 aagatgctag cttcgacatt gtatcagaag tcaatacaga agaagtaaaa aatgcgattc
     8761 agcaaacaac aaaagaattg tccaatcgct ttgacttcaa ggattctaca gtagaaatca
     8821 aattagagaa cagcaatcta gttgtattga gcgatgatga tttcaaaatc gaacagatca
     8881 aagatgtatt atttggtaag ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt
     8941 ccgaatcaga acacgctcta ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa
     9001 tcgatcgtga aaacgcgaag aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa
     9061 aagcacaaat ccaagaagat cagattagag tgacaggtaa gaaccgtgat gatctacaag
     9121 ctgtgattgc tttattacgt aaacttgact taccaatcga gttgcaattc actaactatc
     9181 gttaatcaaa aaacgaggct gacgtctgtc attcctcgtt tttttgattt actgcttctt
     9241 ttgataacca aaaacgctgt attccttctt ccaattcccc aacacttcgt acttctcttg
     9301 ctgtttgcca atgttgtggg aataaacgtc gataagaaaa actagaaaat cgttgatctg
     9361 ccaatacaac gattccttga tccgccatgc cacgaatcac tcgaccacca gcttgcaaga
     9421 ctttgttcat tccaggaagc tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat
     9481 aatatgattt gatcagttcc tgctcatgat tcatctgcgg caaacctaca cctacgatca
     9541 tactgccgat caatcggtcc cctctcaaat cgattccttc tgaaaaaatc ccgccaagta
     9601 cgcaaaatcc aatcaatgtt tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc
     9661 gttcttcctc attcatgtcc gtttcttgga ttagcacatt gctgtcggga tattgctgtc
     9721 tgaatgcttc caccgcaaga tctaaatatt gataagaagg aaagaaaacg aaataattcc
     9781 cgacctttcc atcagcaaaa gcatgaatcg tctctactaa ttttgccaga ctgtgttccc
     9841 tttttcgata agtcgtctct aggtaatttg cgatgaaaac ttgctggttt ccttctggga
     9901 aaggactcgg cagtttgtaa gccagacttt tttgtccccc cagtgtttcc tgataataag
     9961 agagtggtga aaaactagca gaaaaaagaa tactgctttt tcctttatcc aacgactgct
    10021 caagaaacaa gcttggatca atacaaaact cttttactat cagatcgcga tacgtttttt
    10081 cgactgttgt ttcgtagtga tcatcataaa attcactgac cttcaaaaaa tgaagcaaat
    10141 caaaataata aggaagcaat tgttcttgtg atgggtgctc aggaaattct gccagccatt
    10201 ctttaatttt ttcgcttaat tgataacctg cctttactaa agattcagcc ggtgcttttt
    10261 gatgatgata atcccaatga tcttcttttg ctatttctcg gatcgaatca aattctttta
    10321 atagtttgtt gaaacggcga tgaagttttt tgaattcctt tggtatagct tccttacttc
    10381 gtttcgtttt ttcataagat agttctgccg aatacatctc tcgggaacga ttcaccaaat
    10441 tatgtgcttc gtcaatcaaa aacagatagt cctcgttttt ttcatcttca aaaaaacgtc
    10501 ttaaataaac agttggatca aataaataat tgtaatcccc aatgattacg tcacaccaga
    10561 tactgacatc taatgatagt tcgaaaggac aaagtgtatg ctttcttgca taagtttcga
    10621 tgacttcacg agtgatctga ttctcatgat tcaaaagatc ccataaacct tcattaatcc
    10681 gattgtaata accattggca tatgggcact gatctggatt acaggtcgtc tcatctaaaa
    10741 agcagatctt gtctttcgcc gtcaaagtga cactttttac ttcagctcct gtatctttta
    10801 atgctgtcat agcatcttca gctacttgtc tagttattgt tttagccgtt agataaaaga
    10861 tcttttctcc ctcttcttcc cctacagctt tcaaagcggg gaataacgta gagattgttt
    10921 ttcctgttcc ggtaggtgct tctacaaaca atttctgttt cgtacggatc gttttataag
    10981 cagctaccgc tagttcacgc tgacctttcc tatactcatc atgggggaat ttcaaagcca
    11041 tcaaagaggc attgcgtaca gtgcgccagt tttcttgaaa aacaagccat tctttataat
    11101 cttcagtcag ctttttaaaa aaagtttcta actgctctct tgtctgatgt tcaacctttc
    11161 tcgtgatcac ttcttctgcc gtctggaagt aagtcaattg cagatttatt tcttgtaaat
    11221 catgttcttg actatagata taggcataca cccttgcttg atgaaagaaa agttccactt
    11281 gttctggttc taaatcttca aatcgtggtt cggaggtttt gatttcatct atataataaa
    11341 taccgtctct tttgaaaata ccgtctgcac ggccttcaac gatcaggtaa tattcatcta
    11401 attgaactgt tgtttttaaa aagacttctt tttcatagtt ttctcccgca tctttttgga
    11461 gcttgcggtg gattctcgct ccttctaatg cagtatggtt acttttttta cgattgtcga
    11521 tacttccacg tcgcaaaata aattctacta agtgccgcac agatattttt gactgtttga
    11581 tcatttccac cacctctttc tctctaagat tacctgaaaa aacctagttt agcaaaaaga
    11641 gctctctttc caaacataaa aaaacaccct gctgattagg cagggatata ggatatagaa
    11701 tttggatttt tggatgaatc taatgtaaca aggacatatt aaaatgctat gaaaacttac
    11761 tttccttctc tttagaatat ccaaatactt ttcatatagt ctgataattc agataagtaa
    11821 agagaaataa gtacctgttt ttgtttccta gcaatgaatg cgttatacta atggatgaaa
    11881 gaagttttgg ctatcaagga ggaatgcaca tgcatatcga aagattaaaa acaggaatga
    11941 ttgaagaaaa ctgttatttg gtctacaacg aagaagcact tttgatcatt gaccctggag
    12001 aagatgcgga acgaatcaaa caagaaatca aaaaaacaaa ccaacagccg gtagcgattt
    12061 tgcttaccca tactcactac gatcatatcg gtgcagtgga acctttacgc gcgtattatc
    12121 aaatcccagt ttatgtcagc ccactagagc aaaaatggct aggtgatccg attttgaatc
    12181 tttctggact cggtagacat gatgatattg ctgatgttat tgtctcgcct gcagaatatg
    12241 aatttgaaat gaaaacttat actttaggaa acatgtcttt tcgcgtcgtc cctaccccag
    12301 ggcattctat aggaagcgtc agctttattt ttgatgattt tgtcgtttct ggcgacgccc
    12361 tgtttagagg aagtatcggt agaaccgatc tgtatacagg gaatttagaa caattacttc
    12421 atagtattcg gacccagctg tttgtattac ctgatgaatt tgcagtctat ccaggacatg
    12481 gcgatgcaac gacgattgaa caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg
    12541 acttaggagg agaataaaat gacagaaact acactttata tcgtacgaca cggaaaaacg
    12601 atgttcaata caattgaacg agtacaaggc tggtgtgata cacccctcac aaagcaagga
    12661 caagaaggca ttcattattt aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat
    12721 tctagtgaca gtggtcgtgc aatcgaaact gcccgtatta ttctaggaga acattctaag
    12781 ggaaaagaaa ttccttattt tattgaaaaa aggattagag aatggtgttt cgggtctctt
    12841 gaaggtggat atgacatgga aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca
    12901 tatgatgaaa tgtttaccac agaagtttct tttgaacaaa ttgctaatgc tgtatacaac
    12961 ttagataccg ctgggtgggc ggaaacttat gaaacattgc gagaccgtgt atggacagga
    13021 tttgaagata tcgcccatca ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct
    13081 catggtttga cgatcgcctt tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct
    13141 gggcttttaa atggcagcgt aacaaaggtc acttatgcaa acggtcgatt ttctatccac
    13201 ggcatcaatg atacgtctta tatcgataac gggaaaacac atagtcgcga ctcgtttcta
    13261 taatggggat attttaattt ttaccagaga atcataaaag gcaaagtctg aacgagggct
    13321 ttgtcttctt ttttctcttc gtgtacttat ctgctttatt tctcaagttt ttcttaatat
    13381 taaaaaacag attgggtttc tcaaaaaata acggtatact ataagatgta tcttaattgg
    13441 gaaggaagga atttatgttt tttgcaaata ttatcaaagc agtgattctc ggtattattg
    13501 aaggaataac ggaatggctg cccatcagta gtactggtca tttgatccta gcagatgaat
    13561 tcatcaaact ggatgccagt tcacaattta tgtcgatgtt caatgtcgtt atccaattag
    13621 gggcgatctt ggctgtcgtc gttctttatt ttcacaagtt aaacccattt tctccaagta
    13681 aatcaacggt agaaaaaaaa gatacgtgga cactttggtt taaagtgatc gttgcttgca
    13741 taccagccat catcattggt ttgcctttgg atgattggtt agaagcaaat ttccacaagt
    13801 tcttaccagt tgccattgtt ttgatcgttt acggtattgc ctttattatt gtcgaaaaac
    13861 gcaacaaaaa caaaacacct cgttggactt ctctgaatga ctttacgtac caagcagctc
    13921 ttcttgttgg gttattccaa gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa
    13981 cgatcctagg atctatcttg attggtgctt cgcgttttgt cgctacagag ttttcatttt
    14041 tcttagggat tcctgtcatg tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg
    14101 aagggaattc attcaaaatg gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt
    14161 ttgtggtttc cgtcttagct atcaaattct tactaagcta tttgaagaaa aacgacttta
    14221 ccgcttttgg ctggtatcgt attatccttg gtatcatttt gattggttat tggtttattg
    14281 caatgtagaa aaaaccagaa cacttctct
//
LOCUS       DACDSR010000068        14249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDSR010000068 DACDSR010000000
VERSION     DACDSR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.068"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IWK15_002016"
     CDS             173..1309
                     /locus_tag="IWK15_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9547589.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IWK15_002017"
     CDS             1446..1790
                     /locus_tag="IWK15_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9547590.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IWK15_002018"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IWK15_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9547591.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IWK15_002019"
     CDS             complement(3485..4084)
                     /locus_tag="IWK15_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9547592.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IWK15_002020"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IWK15_002020"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9547593.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IWK15_002021"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IWK15_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9547594.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IWK15_002022"
     CDS             7290..8408
                     /locus_tag="IWK15_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9547595.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IWK15_002023"
     CDS             complement(8468..9760)
                     /locus_tag="IWK15_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9547596.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IWK15_002024"
     CDS             9910..10470
                     /locus_tag="IWK15_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9547597.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IWK15_002025"
     CDS             10599..10976
                     /locus_tag="IWK15_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9547598.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IWK15_002026"
     CDS             10909..12981
                     /locus_tag="IWK15_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9547599.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="IWK15_002027"
     CDS             complement(13192..14139)
                     /locus_tag="IWK15_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9547600.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         4634 a         2584 c         3025 g         4006 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattcttt
//
LOCUS       DACDSR010000069        14224 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDSR010000069 DACDSR010000000
VERSION     DACDSR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.069"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IWK15_002028"
     CDS             complement(221..1423)
                     /locus_tag="IWK15_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547601.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IWK15_002029"
     CDS             complement(1426..1620)
                     /locus_tag="IWK15_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9547602.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IWK15_002030"
     CDS             complement(1667..2515)
                     /locus_tag="IWK15_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9547603.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IWK15_002031"
     CDS             complement(2720..3070)
                     /locus_tag="IWK15_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547604.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IWK15_002032"
     CDS             3665..4111
                     /locus_tag="IWK15_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9547605.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="IWK15_002033"
     CDS             4735..5727
                     /locus_tag="IWK15_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9547606.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="IWK15_002034"
     CDS             5743..6627
                     /locus_tag="IWK15_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9547607.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="IWK15_002035"
     CDS             6676..8283
                     /locus_tag="IWK15_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9547608.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="IWK15_002036"
     CDS             8284..9768
                     /locus_tag="IWK15_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9547609.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="IWK15_002037"
     CDS             9945..10937
                     /locus_tag="IWK15_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9547610.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="IWK15_002038"
     CDS             10989..11858
                     /locus_tag="IWK15_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9547611.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IWK15_002039"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IWK15_002039"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9547612.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(13898..>14224)
                     /locus_tag="IWK15_002040"
     CDS             complement(13898..>14224)
                     /locus_tag="IWK15_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9547613.1"
                     /translation="LSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSF
                     KKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQ
                     GLIFEN"
BASE COUNT         4350 a         2412 c         2939 g         4523 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt
    13921 gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt
    13981 taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa
    14041 aggcatttga aatacccgat tggtagcgat ggaagatcgt gaatttcttt ttaaacgact
    14101 gaggtaacgt agatgaaatt gttttaaggg tttcttgaaa gccttccgta cctcgtttaa
    14161 cgaaataata gcgtaataat tgacaaaaat gatacgcagt tcgcaattct tcgttgtagc
    14221 ttaa
//
LOCUS       DACDSR010000070        13746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDSR010000070 DACDSR010000000
VERSION     DACDSR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.070"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWK15_002041"
     CDS             139..477
                     /locus_tag="IWK15_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547614.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWK15_002042"
     CDS             complement(682..1257)
                     /locus_tag="IWK15_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9547615.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IWK15_002043"
     CDS             complement(1989..2570)
                     /locus_tag="IWK15_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9547616.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IWK15_002044"
     CDS             complement(2753..3379)
                     /locus_tag="IWK15_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9547617.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IWK15_002045"
     CDS             complement(3401..4732)
                     /locus_tag="IWK15_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9547618.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IWK15_002046"
     CDS             complement(4887..5288)
                     /locus_tag="IWK15_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9547619.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IWK15_002047"
     CDS             complement(5374..5580)
                     /locus_tag="IWK15_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547620.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IWK15_002048"
     CDS             complement(5675..7177)
                     /locus_tag="IWK15_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9547621.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWK15_002049"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWK15_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9547622.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IWK15_002050"
     CDS             complement(7748..8365)
                     /locus_tag="IWK15_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547623.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IWK15_002051"
     CDS             8586..9959
                     /locus_tag="IWK15_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9547624.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IWK15_002052"
     CDS             10034..11146
                     /locus_tag="IWK15_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9547625.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IWK15_002053"
     CDS             complement(11218..12855)
                     /locus_tag="IWK15_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9547626.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="IWK15_002054"
     CDS             complement(13023..13631)
                     /locus_tag="IWK15_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9547627.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3718 a         2890 c         2459 g         4679 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatt
//
LOCUS       DACDSR010000071        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDSR010000071 DACDSR010000000
VERSION     DACDSR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.071"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWK15_002055"
     CDS             complement(<1..914)
                     /locus_tag="IWK15_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9547628.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWK15_002056"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWK15_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9547629.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWK15_002057"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWK15_002057"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9547630.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWK15_002058"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWK15_002058"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9547631.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWK15_002059"
     CDS             4440..5321
                     /locus_tag="IWK15_002059"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9547632.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWK15_002060"
     CDS             5318..6034
                     /locus_tag="IWK15_002060"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9547633.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWK15_002061"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWK15_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9547634.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWK15_002062"
     CDS             6751..7752
                     /locus_tag="IWK15_002062"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9547635.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWK15_002063"
     CDS             7749..9110
                     /locus_tag="IWK15_002063"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9547636.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWK15_002064"
     CDS             9070..10221
                     /locus_tag="IWK15_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9547637.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWK15_002065"
     CDS             10196..11365
                     /locus_tag="IWK15_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9547638.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWK15_002066"
     CDS             11540..12919
                     /locus_tag="IWK15_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9547639.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWK15_002067"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWK15_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDSR010000072        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDSR010000072 DACDSR010000000
VERSION     DACDSR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.072"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWK15_002068"
     CDS             complement(14..706)
                     /locus_tag="IWK15_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9547640.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWK15_002069"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWK15_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWK15_002070"
     CDS             complement(1143..1712)
                     /locus_tag="IWK15_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9547641.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWK15_002071"
     CDS             complement(1786..2109)
                     /locus_tag="IWK15_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547642.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWK15_002072"
     CDS             complement(2263..3075)
                     /locus_tag="IWK15_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547643.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWK15_002073"
     CDS             3429..4277
                     /locus_tag="IWK15_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9547644.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWK15_002074"
     CDS             4422..5363
                     /locus_tag="IWK15_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9547645.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWK15_002075"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWK15_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWK15_002076"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWK15_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9547646.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IWK15_002077"
     CDS             complement(9827..12013)
                     /locus_tag="IWK15_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9547647.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWK15_002078"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWK15_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9547648.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IWK15_002079"
     CDS             complement(12235..12675)
                     /locus_tag="IWK15_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9547649.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IWK15_002080"
     CDS             complement(12749..13288)
                     /locus_tag="IWK15_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9547650.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDSR010000073        13197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDSR010000073 DACDSR010000000
VERSION     DACDSR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.073"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..1825
                     /gene="yfcC"
                     /locus_tag="IWK15_002081"
     CDS             86..1825
                     /gene="yfcC"
                     /locus_tag="IWK15_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9547651.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1982..3166
                     /gene="iadA"
                     /locus_tag="IWK15_002082"
     CDS             1982..3166
                     /gene="iadA"
                     /locus_tag="IWK15_002082"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9547652.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3277..3480
                     /locus_tag="IWK15_002083"
     CDS             3277..3480
                     /locus_tag="IWK15_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9547653.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3581..4243)
                     /locus_tag="IWK15_002084"
     CDS             complement(3581..4243)
                     /locus_tag="IWK15_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9547654.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4256..5758)
                     /locus_tag="IWK15_002085"
     CDS             complement(4256..5758)
                     /locus_tag="IWK15_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547655.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6306..7985
                     /locus_tag="IWK15_002086"
     CDS             6306..7985
                     /locus_tag="IWK15_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9547656.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8112..8591
                     /locus_tag="IWK15_002087"
     CDS             8112..8591
                     /locus_tag="IWK15_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9547657.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8810..9490
                     /locus_tag="IWK15_002088"
     CDS             8810..9490
                     /locus_tag="IWK15_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9547658.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9498..10829
                     /locus_tag="IWK15_002089"
     CDS             9498..10829
                     /locus_tag="IWK15_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9547659.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10844..11806
                     /locus_tag="IWK15_002090"
     CDS             10844..11806
                     /locus_tag="IWK15_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9547660.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11917..12255
                     /locus_tag="IWK15_002091"
     CDS             11917..12255
                     /locus_tag="IWK15_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9547661.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12334..13041
                     /locus_tag="IWK15_002092"
     CDS             12334..13041
                     /locus_tag="IWK15_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9547662.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4189 a         2333 c         2899 g         3776 t
ORIGIN      
        1 gaaatgtcat ttttactgta taattcattt gattatccat tttttattca tcaaaattac
       61 tgaaaaaaga aaggaagaaa taaatttgaa gaaaacaaat tcttcaggaa aaccgaaacg
      121 cgacttcact aaattatcga caccccatac gtatgtcatt atttttggtg tagtcatctt
      181 tgcatggata ctgacttttg tcgttccagc aggaaagttc agcacgcaag atattgaata
      241 caaagatgca aatggcgaaa caagtactcg tactgtttta cgtcaagatt ccttccgtta
      301 tgcttacgag ctggataagt cgtatgtgtt tgatcaatta gaagaactgc aagatcatcc
      361 agcagaacgt gaaaaactgg atgttccgga aaaaggattg gaaaaagtca ttgctgacgg
      421 agaaaaaaat cttacgcagg aaaaactaga tgaaatctcc ttgacagatg atgtgcttta
      481 cgatcagtac ggcgaaaata tctatgatac ttctaaaaaa ttgcataaaa cagccaaaat
      541 atggggaaca gatgattttg gcggctttgg tttcttaaat tttgtttttg aaggactagt
      601 atctggagat aagtatggat ctgctgtagg gatcgctgca ttgatccttg ttgttggagg
      661 tgcttttggt attattatgc gtaccggagc gatcgacgca ggtatctatg cgtttatcag
      721 taaaactaag ggactggagc gtttagcttt accgctatta ttctttgctt tttcattcgg
      781 tggcgctaca ttcgggatgg cagaagaggt aattccattc tcaatggtga tggttccatt
      841 tgtcattgcg ttaggatatg actcgatcgt agcagtaacc gtgacttatg tcgcatctca
      901 agtcggaaat gcgacctctt ggatgagccc gtttagcgta gcggtggctc aagggattgc
      961 tggaatcccc gtgttgtctg gtgcgacatt ccgattgatt atgtgggtag ttgttactgc
     1021 attagccgct ggttatatga tgatatatgc agaaaaaatc cgtaaaaaac cagaaagttc
     1081 gttgacttac aaatcagatg attatttccg ttctcatatc aaaaagacat ctgatgaaaa
     1141 taaaaaattc atgcttgggc ataaattgat cttattagag atgttagttg tccttgtctg
     1201 gatcgtttgg ggcgtgactc aaaaaggcta ttacattcca gaaatcgctt ctcaattctt
     1261 tgtgatggga ttagttgctg gaatcattgc tgttcttttc aaattagatg gaatgaaagt
     1321 caatgacatc gcttcttctt tccaatctgg tgcagctgat ttagctggta cagcaatcgt
     1381 agtaggtatg gccaaaggga ttcttcttgt cttgggagga tctgatgcat ctgttccttc
     1441 agctttgaat acgattttgc atgggatcgg gacggcgttg accggtgttc cagctgtcat
     1501 tggtgcttgg gcgatgtaca tcttccaaag tttgttcaac ttggttgtta catcaaattc
     1561 aggacaagca gctttgacta tgccaatcat ggctccactg gcagatttag taggtgtatc
     1621 cagacaagta gcagttcttg cttatcagct tggttcaggt tttatggacg cttttacacc
     1681 cgtatcagcc agcttgattg gtgtgctagg tgttgcacgg atcgaatggg caaaatgggc
     1741 aagattccaa atcaagatgc aaggattcct gtttgtttta ggaactattt tcatcatgat
     1801 tgccattgca ataggtctgc agtagtactg gagacctgta aataaaaaag taatccaaac
     1861 agtagataag gactccaaga accgtcattt tacggtaaaa tcaaaggagt aaagatccta
     1921 tcgtttggat tattttttaa gatcctatca tcagttatac ttaaagaaag aggaaaaatg
     1981 tatgaaaatc attcgacaaa ttgaagtgtt tgcaccagat tacttaggaa aaatggatgt
     2041 actgattgcc ggtgggaaga ttttggctgt agaagaacag ctagaaggcg gctacgaagg
     2101 agtggaagta gaagaacttt caggtgaggg aaaagtacta accccgggtt tcattgactg
     2161 tcatttccat attttaggtg gaggaggaga aggcggttat caaaatcgaa cgccagaagt
     2221 cacattgagc caattgacta cagctggtgt aacgacagtt gtcggttgtt tgggaacaga
     2281 cggagaaggt agagatatga ctgcgttgat cagcaaagcg aaaggtctgg aagcagaagg
     2341 aatctccact tatgtatacg aaggttcgta ccgattgccg gtaaaaacag taacagattc
     2401 tatcatcaaa gactttttga caattgataa aattatcgga atcggagaaa tcgcagtatc
     2461 cgatcaccgt tcttctcagc caagttttga agagttcgct cgtgcagtag cagatgctcg
     2521 tgtaggaggc atgttatccg gaaaagctgg gatcatcaat gtccacttag gcggaggaaa
     2581 aagaaagcta gagctattga cacgaatcgt agaagaaaca gaaattccga tcacacaatt
     2641 tttgcctact catgcaaatc gcacaccaga attatttgaa gcttgcgtgg catttgccaa
     2701 acgtggtggc acgattgact tcactgctag cgaagatcct gatttttggg agaagacaga
     2761 tggtgaagtt cgcttcagca aagcactgaa acgattgatc gaggaagatg tttctttaga
     2821 taatttcacg atgacctcag atggacaagg aagcttgcct tattttgatg aaaacaatca
     2881 tttcctaggg ttaggagtag gaagtgccaa ggcattgcta gtagggatca aagaagctgt
     2941 gcaaaaagaa agtatccctt tggaaatcgc cttacgagct ataaccagta atccagcacg
     3001 tatattgaaa ttggataaaa aaggaaaaat cgaaattggt gctgatgcag atctgtgtat
     3061 cttggataaa gaaacgcttg atattgatac agttatcgct aaaggagaaa taatggtcca
     3121 agaaaaagaa gtcaaagtat gggggacttt cgaaaaaagt ttttaatcaa cagctctaac
     3181 tatctttttt agaaaacgag cgttctgttt gtctgtatct gttgttgatg ttaccctgaa
     3241 ggtagatcaa taaacaaact aagaggagga agcacaatgg cagatttaaa aggacgttct
     3301 aaagatatga aagataaagt agtcggtgaa ggaaaagaag catacggcaa agcaacagac
     3361 gacaagagca aagaaacaga agggaaagca caatcactag gtgctgacat caaagaaaaa
     3421 gctcgaaatt taggcgacga agtcaaagac aagttttctc gaaaagacga aaaaaaataa
     3481 aaaccactta ttactaattg aatcaagcaa ataaaaaatc cgaaccatat ctgcgagaca
     3541 gatatggttc ggatttttta tttgatcatt atcctgtttt ttaaacttcg attttttgaa
     3601 attttttatt ttttagttca aagacttcat cacagctttt tgctacttca atctcatgag
     3661 tcacgatgat aatacagcga ttttgttcat gggcgatttc ttggaaaagt ttgacgatat
     3721 cttgtgaagt tgtttcatct agatttccag taggctcatc cgctacgatt agttgatgtt
     3781 cacagcagat agcacgaacg atagcgactc gctgctgctg acctcccgaa agtttggtca
     3841 cctgtttttt tgctaatatt tcatcaattc ctacacgagc cagtcgatct aaagcaactt
     3901 gtcgataatt ctcttgcttg gtcttagtga tttccattgc tgctatgacg ttgtctagag
     3961 ccgataaata aggaagcaag ttgtaagctt ggaaaataat cgagacatca tttttacgat
     4021 aacttcttaa cccgattttg ttcagtgaac gatcttgata gtataccttt ccttcttttg
     4081 gtgcatctag tccagaaata agcgaaagaa aagtcgtttt tccagaacca cttgctccta
     4141 aaatagcgta catttttcca gcttcgaaat tcagttctac atctttataa agcgcatctt
     4201 gcggatcttg ataccaaaat cccaattgtt ctgtccgtaa catcaaattc ctcctttagt
     4261 ttaataggat ttttcttggg tttaatctta atatccctat agaagctaaa aagacagaaa
     4321 acagactgat tccaaatgct actccggcta atagtcccaa ttgtgcaggt ttgacattga
     4381 tttccaattc gctgacttga tcagaaacag caaatggatt attcgtggtt ccttgttgac
     4441 catcgcgttg accgccttgt tcttgtctgt tacctggcat ttgtccaccg ccaggagctt
     4501 gctggttttg tgtcgtggtt tctgtctgtt ggtcaagtag ctgctgtccc acggcattcg
     4561 cgacgacgtt tccgctaagc gaagcaactc ctaatgcaag aatcatacaa atagccactt
     4621 cggtaaacaa ttggagaatg attttacttc tggattctcc taaagatagc aagacgccta
     4681 actcatgccg acgctcccga atggtaatca taatgatcag agtaagtatg ataataccgg
     4741 ctacagccac tagtaaaaca acatttttag aaaaacttgc cacgttgttt aaaggttcca
     4801 acattgactg atacatggaa tcatttgatt gtaagctaaa tgtatctgtg tcgatcagct
     4861 tctcagcttc tttgagaaac tcatccattt tatctggatc agatagagtg taaatagcag
     4921 aatctatcgt gttatcgtca gatgtcccat ttagttcatt tgcaaaagtg taagaagtgt
     4981 aaagtgtatt cgacggattc atgaagttga atttcatacc catactgccg cttgtttcgc
     5041 tggattcata aattcctttg atcgtcattt cataagtggt atcttcatct tcagtactcg
     5101 tgattgtgaa agtatctccg actgccaggt catttgtttc tgctagagta gaatcaatga
     5161 ctacattgtt tgtgcctttg tcgctgtcat ctattccttc accatccgtg atttttgctg
     5221 ttccatcaga aaaattacta ttttgtgcag ttgctgacac accactgatt tgaaagtctg
     5281 cttgagtcat ttgcggattc ccgcccattc ctccagcagg ttcaccgcct gcatcagtag
     5341 tttctgaact gtcttcactt gaatcagaac tagtgatggc agttattcca tcttttgcca
     5401 tagctgaggc ggaactttca aaggaataac tctttacatt atctaattca gctatttttt
     5461 ccgcgtccgt taaggaaact ggtgtaagag aaaaactgcc tggatctgga cgttcttcag
     5521 tggtagaacc gcttgaagaa gtttgttcct gttttttaaa tgaagcttct cgattggcac
     5581 ttaaagtcac tgttgctcca acttcttttt gtgcttgatt cgctgcttct tctgcagcgc
     5641 ttcgaatcgt gaggccagct agcacaaaaa taagaatggc tgtgaacacg gcaaacaata
     5701 acaatgaacg tccccatttt acttttgtac ttttccatgc acgtttcata aaattcatat
     5761 tttgcatccc tcctagtaga tgcatttgat aatagacaac ctatcttaaa gctagcttaa
     5821 atcttatgct gtttgcacac catcaacatg aggttctgta tatataaaaa gtcactatag
     5881 ttattgcaaa aaatgtggta aaatgaaaaa agtatgtttc aaaccgacga agaagaaatg
     5941 aaagaggaat agcaaaagaa atcctagatg ctacagaggg atttttacgt gcatagttgt
     6001 tctaacaatt gctgatacta gcgatttagt ttatttgttg tataataatg gggaacagct
     6061 gaatacttat ataagtattc tggctatagc gtttcgcaaa agatccattt tgttaatgaa
     6121 gtatatccaa aataaaatgc ccatccaact gtcagaacgc ggatgtgctg gtaattttcc
     6181 tgctgattct ccataagttg agaaatagtg attttgcata tgcttcattg gaatggctca
     6241 tatatttaga taaagaagaa aatcgacatc tttaaacttg gatgtcataa gaagaggtga
     6301 agtttgtgag taccattaaa attatcccat taggcggcgt tcgtgaaaat ggaaaaaaca
     6361 tgtatgtcgc agaagtgggc gaggatattt ttattttaga ttgtgggcta aaatatcctg
     6421 aaaatgagtt gttggggatc gacgtcgtaa tccctgattt tacgtattta gaagaaaaca
     6481 ttgatcgtgt agctggtgtc tttttaacac atggacatgc agatgcgatc ggggcacttc
     6541 cttacttgtt gtcaaaaatt tctgtaccag tattcgggac agagttgacg atcgagttag
     6601 caaaattgaa tgtcagtcgt aatgaagcag ccaaaggatt taaggatttc catgtggtag
     6661 atgagcatac agaaatagac tttggtgaaa cagtagtgag ctttttccgt accactcata
     6721 cgataccaga ttcagtgggg atcaatctga aaacaaaaga aggtagtatc gtttatacgg
     6781 gagattttaa atttgaccaa acggctatcc caatgtatca aacagattat gcacgattgg
     6841 cggaaatcgg caaagaagga gtcttagcct tactaagtga atcggcaaat gcagaaaatc
     6901 ctcagcctgt agcttctgaa gcccaaatag cagatgaagt atttgatacg atcaaatatt
     6961 gggaagggcg tatcatcgtc gcttgtgtag caagtaattt gcaacgagtg caacaagtat
     7021 tggatgcagc agaaaaagca caacgaaaag ttgtcttgac tggtcaagat ttcgaacgaa
     7081 ttattcgcac agcgatgcgt ttagaaaaac ttcaattacc aagtgaagat ctgcttgtta
     7141 cgttgaaaga catgaaaaac tatgatccag aagaactgat cattttagaa actggccgaa
     7201 tgggtgaacc catcaagtct ctgcaaaaaa tggcaaacgg tacacaccgt aatgtcaaga
     7261 ttgaagaagg agatttggtt tatatcacta ctacgccaag catcgcgatg gaaaccatcg
     7321 tggcgaaaac ggaagatatc atttatcgag ctggtggaac agtgaagttg atctctgaga
     7381 atatgcgcgt ttctggccat gccaatccta atgacttgca attaatgatc aatttgatga
     7441 aaccaacata ttttgtgcca gttcaaggag agtatcgtga attagctgct catgcagatt
     7501 tggctcatgc agtagggatg ccatacaaaa atatctatat tactggacgc ggagacgtgc
     7561 tggaatataa gaacaagcgt atgagtgttg caggaactac aagtgcagaa aatgtaatga
     7621 ttgatggatt aggcataggt gatatcggaa acattgtttt acgtgatcga aaaatcttgt
     7681 cagaagacgg gattttcgta gcggttgtca ctatcagtcg gcgagaaaag aaaatcattt
     7741 caaaacctca aattacttca cggggattcg tttacgtaaa agctagccgt gatctgatga
     7801 aagaaagcag caatatgatt gaaacaatcg ttgagaaaca tttagaaagc aatgattttg
     7861 agtggagcaa attaaagcaa gacatccgag atcaattagg ccgttactta tttgaacaaa
     7921 caaaacgccg cccagtgatc ttgccagtca ttatggaagc tactcaacgt cgaaataaaa
     7981 aataaacaaa aagagtaaga tgaacctctc tatagagtgc tcgtcttgct ctttttttgc
     8041 gaaaaacaaa taatttttga atacagcatc cctcgcttta taataaacga tagaataaag
     8101 ggagtgaatc aatgatgagt caaacaaaag tcgaacgtta tttagaagaa caaaagatca
     8161 gctatcaacc acttgatttt agtcatgtgg tagaagaatc tttcacagaa gaactacaaa
     8221 aaagagggat tgatccagct ttgatatgta aaactctcgt agtgaaagga aacaaaactg
     8281 gggtgatcat cgcattgatt cctttgaacg atcacttaga ttacaaaaaa acgagacaag
     8341 tgacgaaaga tcgtaaagtt ggttttcctg gaatggattt tgtattaatg catacaggat
     8401 atccccacgg agcaaacacc cctattggaa taaaactcgc acatcctgat tattgttttt
     8461 tagcagacag ttctttaaaa gaaaaagaag aagtaattgt gtcaagcggt gaaatcggga
     8521 gaagccttcg gataaaagta gccgatttag aaaagatcgt ccatcctatt tacgctgatt
     8581 taattagtta agggacgaga agagaagcta agcagagcag agacctcaaa cttgagatta
     8641 tatctgcttg gtacttggtg catcctgtat tatctgttac aattgctttg tacatactgt
     8701 actatcaatc caatgaaaat atatttagca gctatatttt ttcattgatg tgcaagtgat
     8761 gaaatcgttt ttaataaaaa agatacagag aaaacgcgag gtgtaaaata tgaaaattac
     8821 gtatcatgga cattcctgtt taacaattga aatggaaaat ggccaacgcg tgatgattga
     8881 cccgtttatt acaggaaatc cattgacaga cttaactgca gaagaagtaa agacagattg
     8941 gttgctgatt actcatggtc atagtgacca cataggagat atgattccta ttgcaaaaga
     9001 aaatcaagcc acaattatca gtattgttga gattgctgat tatgcgaaca gcagaggagt
     9061 agattcattc ggtatgaata tcggtggaaa acatgcgttt ccattcggcg tggtaaaatt
     9121 cgtacatgca cagcatagtt caagctatga agtagacggt atcgttcagt atatgggcga
     9181 gcctagcgga attattattc aagcagaagg aaaaacgatt tatcatgcag gggatactgc
     9241 ttatttcagt gatctaggat tgcttgcaga agaattcgat attgatgtcg ctttcttgcc
     9301 gattggcgat aactatacga tggggccaga agatgcagca aaggctgcgc aaacaattcg
     9361 tgccaagaaa gtagttccaa tccattacaa tactttccct gtcattgaac aagatcccga
     9421 atcttttgta gagttactgg aagatagtga aggaaaaata atgagcgtct cggaaacgct
     9481 ggaagtgtag gcagaaaatg gcgacaaaac atgatttgat tttagaatat attgaaagtt
     9541 tacctgttgg caatcgaatt tctgtaagaa gcatcgcaaa agaattgaat gtcagtgaag
     9601 gaacagcgta ccgtgcaatc aaagatgcag aaaatgtcgg actagtttca acaattcaac
     9661 gagtagggac catccgtatc gaacgcaaat taaaaaaaca tattgaacgt ttgacatttg
     9721 gcgaggtcgt caggatcatt gaaggggacg ttttaggcgg ggcggcagga ttagacaagg
     9781 tcttgaataa attcgtcatc ggcgcgatga ctaaacatgc catggaacgc tatattacgc
     9841 caggatcact aatgatcgtc ggcaaccggg cggaagtcca aaaattagca ttggaagatg
     9901 gcgccgcagt attgatcaca ggaggattca atacgactgc tgagattgca aagctagctg
     9961 atgatcttga aatgccgatc ttacgcacct cttatgatac atttacagtc gcaaccatga
    10021 tcaaccgagc gttaagcgac caattgatca aaaaagatat catgcttgtc agtgatatct
    10081 atatgccagc tgaaaagact cattatttgc agcagacaga tacgatttct gattatcagc
    10141 gtttgtcaga agaaacgcag cattcgcgtt ttccagtcgt caaccgccat catcgtttga
    10201 tggggattgt tacggcaaaa gatgttttag ggaaatcgcc aaaccagatc atcgatcggg
    10261 tgatgacgaa agaaccaatc agtgtaaaga aaacaatgag tattgcctct gttagccatc
    10321 aaatgatctg ggatgggcta gaagtcatgc cggtcgtttc cgatgacttg acattggaag
    10381 gcttgattac ccggcaagat gtaatgaaag cgatgcagct tgtccagcga caaccgcaga
    10441 ttgcagatac gatttcggat cagatatctg gaaatatcca tacgattgat actgaccgtg
    10501 aaggaaatcg tttggaaaac cctaagttca aattcgtagt agcaccacaa atggtcaatg
    10561 gcgttggaac catttctttt ggcgtactta gcgaaatcat ttctgatgtc gcacaaaaaa
    10621 caatggtgat gaaccaaaaa cgaaatatct tgattgaaca agtgaaccta cattatttgc
    10681 ggctgatcca attggaaagc gaattagaca ttcgtccacg tgtattggaa atcggcagac
    10741 gttccgctaa actggatatc gaagtgttta ttgaaaacac catcgtttcc aaagcaatcg
    10801 ttgtttgtca agtaatggaa cgttcttaga aagcaggagt gaaatgacag tacagcaaga
    10861 aatagtagat acaataaaaa aatacgataa aatcattatc cacaggcatt tgcgccctga
    10921 tcctgatgca ttaggatcac aagtaggatt agccgaacta ttacgggaaa gtttcccaga
    10981 aaaagaaata cgtcaagtag gtgaaacgat tgaagggctt cgctttttag cagaaatgca
    11041 agaagttgaa gatgtcttct accaaggagc gctagtcatc gtgactgata cagctaattc
    11101 acccagaatc agtgatcaaa gatatcagtt ggcagaaaaa ctgatcaaaa tcgaccatca
    11161 tcccaatgat gacccttatg gagatcttgt gtgggtaaat acaaaagcaa gtagctgtag
    11221 tgagatgatt gcagaatttg ctttgatgtt tcctgaatct ttaaagatga acgttaaggc
    11281 tgcacggtta ctttatgcag gaatcgtcgg agatacaggg cgctttttgt atccctccac
    11341 gacgtcccgt acattagaga ttgcttcgat attgagaaat tatccatttg atgcaagtgc
    11401 tttgaacaga gagattgaac aaatgccgat gaaagtcgct aaactttccg gttatcttta
    11461 tcaaaatatc caggtggatg aaaatggtgc aggaagaata atccttccgc aagaaattct
    11521 tgataaatat ggaatcgatg attcagagac agcttcgatc gtttcacttc ctggagtgat
    11581 tgacgaagtg ctggcttggg ggatttttgt tcagcagcct tcagggtatt accgcgtacg
    11641 tttacgttct aaggggccga tcatcaatga attggctaaa cgccatcatg gaggaggcca
    11701 tccattagca agtggtgcga atgcaaaaga tttagcagaa gtagaggaaa tctataaaga
    11761 gatccaagat atatgccaat cgtataagag cagaaaaaca gaatgatact tcgttcgaaa
    11821 acgtgccttt tgtagtaaaa atcacaaatg tatcttttca agatcttctt tttccgttac
    11881 aatagtgctc actaaagatt ttaaaggaga aagaagatga cattaccaaa ttgcccagaa
    11941 tgtggctcag agtacacata tgaagatcga ggcttgttga tttgcccaga atgcgggaac
    12001 gaatggaaca aagaagaaca agaaactgtc atagaagacg gattagtggt attagatgca
    12061 aatggaaacc gtctgttaga gggcgatagc gtgacagtga ttaaagactt gaaagtaaaa
    12121 ggtgcgtcag gtgccattaa acaaggaaca aaagtcaaga acatccgttt agtagaaggc
    12181 gatcataata tcgattgcaa ggtagaagga tttggaccga tgaaactgaa atcggaattt
    12241 gtgaaaaaga attaaagaat attcaaggtc ttttcacatt cttttcctac tactaatgat
    12301 aaaataaagt aaacgaaaag gagtgaaacg aaaatgtatg gttatggaat gtatggtttt
    12361 ttagatccga catttctttt ggtcattctt ggattagtta tttccggaat tgcgtcagcg
    12421 tacgtcaata gtactttccg aaaatacgat caagtgagaa gcaaaaacaa tgtgactggt
    12481 acacaagcag cacaatatat attacaaagt caacagataa ataatgtcgg tgttcaacaa
    12541 attgccggag atctcacaga taactacaat tcaggtaata agatgttgag tttgtctcaa
    12601 gcaactgcac agtctacttc agttgctgcg ataggtgtag cagctcatga atgcggacat
    12661 gcggtacaag atgcttcggg ttatgtccca ttgaagattc gtgcagcact tgttccagca
    12721 gctaactttg gttcgatgat ttcgttcccc ttgattatgg taggtgtatt gcttagctgg
    12781 aactctacgt taatcaatat cgggattttc gctttttctt tagcattact tttccaattg
    12841 gtgacattac cagtcgaatt taacgcgtct cggcgagcaa tccaaatttt gagtgaaggc
    12901 ggactactga cggaagaaga agtaccgatg gcaagacacg tattatttgc agcagcactg
    12961 acttatgttg cagcagcgct ttcgacattc ctgcagctgt tgcgcttgat cttattgttc
    13021 ggcggcaacc gaagagatta aaataaagaa gttgtgaaat cagcgctttg atctgagcta
    13081 ataccgagag ttagctgatt gaataccatt tatttggaat aagggctctt tgtcaataag
    13141 gactgatggg ttgtacaaaa tcaaatctgg gtcagaatga acctcattct ggctcag
//
LOCUS       DACDSR010000074        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDSR010000074 DACDSR010000000
VERSION     DACDSR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.074"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK15_002093"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK15_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9547663.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWK15_002094"
     CDS             complement(322..891)
                     /locus_tag="IWK15_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9547664.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWK15_002095"
     CDS             1077..1979
                     /locus_tag="IWK15_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9547665.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK15_002096"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK15_002096"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9547666.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK15_002097"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK15_002097"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9547667.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK15_002098"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK15_002098"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9547668.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWK15_002099"
     CDS             8547..8732
                     /locus_tag="IWK15_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9547669.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWK15_002100"
     CDS             complement(8794..9990)
                     /locus_tag="IWK15_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9547670.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWK15_002101"
     CDS             complement(10160..11596)
                     /locus_tag="IWK15_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9547671.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWK15_002102"
     CDS             11889..12698
                     /locus_tag="IWK15_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9547672.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDSR010000075        12563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDSR010000075 DACDSR010000000
VERSION     DACDSR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.075"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1227)
                     /locus_tag="IWK15_002103"
     CDS             complement(<1..1227)
                     /locus_tag="IWK15_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9547673.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
     gene            complement(1224..3413)
                     /locus_tag="IWK15_002104"
     CDS             complement(1224..3413)
                     /locus_tag="IWK15_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9547674.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(3547..4737)
                     /locus_tag="IWK15_002105"
     CDS             complement(3547..4737)
                     /locus_tag="IWK15_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9547675.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(5609..7240)
                     /locus_tag="IWK15_002106"
     CDS             complement(5609..7240)
                     /locus_tag="IWK15_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9547676.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(7254..8927)
                     /locus_tag="IWK15_002107"
     CDS             complement(7254..8927)
                     /locus_tag="IWK15_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9547677.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(8940..9257)
                     /locus_tag="IWK15_002108"
     CDS             complement(8940..9257)
                     /locus_tag="IWK15_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9547678.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="IWK15_002109"
     CDS             complement(9282..10682)
                     /gene="lacG"
                     /locus_tag="IWK15_002109"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9547679.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10919..11704
                     /locus_tag="IWK15_002110"
     CDS             10919..11704
                     /locus_tag="IWK15_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9547680.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            11707..12399
                     /locus_tag="IWK15_002111"
     CDS             11707..12399
                     /locus_tag="IWK15_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9547681.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
BASE COUNT         3839 a         2583 c         2094 g         4047 t
ORIGIN      
        1 taaaatatcg tttgcaatta gctttcttct tcgaaccttt tcacctgatt cagtaaagta
       61 caaaattgct aacttgaaaa ataaactatg attatttacc ttaattggtg atatatttat
      121 agcatcaaaa ttgcaatacg gtaattcttt ctttaaatac tcacccagtg aaaacagagg
      181 atagtgttga ggagcaagta cacaaatatc tttttcatga atgccgcttt caatcttttc
      241 ctttattata ttttttatta tgttgggtaa atcctcctta gtaactttgc aatcataact
      301 tataaaacca cggtcgtttt tattattccc tctgctttca atctcgtaag gacaactctg
      361 aaaattagag tagaagtcta ctaatctctg attagacctg taacagccat gtagcgtctt
      421 tgattgaaaa gtgtgactag ttagcatttc tagttcttca actgatttag ctataccatc
      481 taatgatcca taaattgctt ggtcaatatc tccaacaaac attgactgaa tttgagggtt
      541 gttttgaagt aatttagcta ttacattgta ttgtaattct ctagtatctt gaaattcatc
      601 cacataaatc gcccttatat tactagcaat attacgtgca acgttactgt ttacagttaa
      661 aattttatac gctactagta aaatcatatc aaaatcaatt tctttatttt tttccaacat
      721 agtgtgatac atttcagaaa cttgagtata ctgaacttca ttaattttaa aatcatgaga
      781 aagtgacaaa tcaattttgt tcagttcata cgttttgaga tttaaattca gcatattatt
      841 aatttggctg acgtaagctc tttgagtata atcatcaata attgtaaatc cttttgatac
      901 tcttgagaga ttcattttaa aaggataaat aataaattca agacaaaact gatgtattgt
      961 ccccacccaa atattaatat catcatctat actaagtaaa cttaggcgtt ccttaatttc
     1021 ttcagcagct ctattagtgt atgtgatagc aattattctt tttagtgatc catcgtgttt
     1081 aattgcttgg taagcaattt tccttgtaag tacccttgtt ttcccactcc caggacatgc
     1141 atttacataa aggttatctt cactttccac aacatctttt tgttctaaat ttaaatcatt
     1201 tacgattttt tcgaagatat ttgtcatttt gaaacctcag cataaagtat ttttgtgtaa
     1261 ttttctatct gattgattag atacacaaca tcctcatctt taccaaagta ctctaataaa
     1321 gaaattattg ctatattcaa atcagctgta tcacttgttt ttaaactagc tgataaagct
     1381 gacaattctg tagtttcctc gtacaagtcg attgtatgga gtagaatttt tttataaatt
     1441 gagagatcta ctatttcctg acatgcaaaa gcaatagcat taagaatata cttaggaatt
     1501 ttgattaaaa agtcaatttt ttcacctaac tcaattgcaa accacccctt accaacatga
     1561 ttggctagtt ttaacatcgc taaatttctt tctaaatatt gttcactttg tatttcttct
     1621 ttataagcag ttattgtttt tttatctttg aatgcgtcat ctataattgg actgacataa
     1681 gttagaatat tttctttatt cacatctaaa aactcaattt caaatgttgt attagcataa
     1741 aaacaattaa cccaattgtt ttcattaaaa agggaggata atttttcttt tcttgcttga
     1801 cctttctctt gtgcagtgct tttatacaat cgatgttctg atgggattgc ctgttcatct
     1861 aaatcagtta caatagcaca ctttctctgt attctatcat cgtcaaacaa gctagcgata
     1921 tattcaaacg aagtactacc aacattaatc aaacctattc caagttcgtc taagtttata
     1981 ccaaataatt tttttactat ttgaggaata agtatttctt cagcatctcc ttcaactaag
     2041 ataacacctt tagaaaaaag caatccattt cttttaacat ctaaatatct ctctactgcg
     2101 tttattagat ttagattgtt cttttttaac ttttctttag caaatgagtt cagattattt
     2161 gctggattca taacgataga tttgccatta aacgatttta atacattcat ccgagatatt
     2221 tcagacactt cagctaaatg tacagaatgt gtggtcatga gaatctgagt atagtttttt
     2281 tgaagattta atccttcgaa cagagttttt tgaatatgat gatgaatatg agcttctggt
     2341 tcttcaatca acataatatt aagtagttct cttgaacgac acgcttcaaa ttcgactatt
     2401 ttcaaagcta aataaatcat atttagatgt cctaacccta gtaagtctaa gtcgatattt
     2461 tgttctggct tcattgaaag aaattttgct aaagaaccca tatcatctga catagttgag
     2521 gaaagaagaa gattaggaga atacactgaa ccaattatgc tatttagttg ccgattaata
     2581 ttttcgccaa ttttcctaat ttcaggaaca ctggtaatag tctcatttaa ttgctgtata
     2641 attgttttta atgactcaac attatctgat gatatttttg attcaattgt ttccatgatt
     2701 cttttgactg ggtttcgatt gtttttcatt tctctaagaa catctcgaag agcgtcaaca
     2761 taaacaaccg aaatatgctt gaaaatatct gacatgtcaa ttcttgagcc caacagagct
     2821 tcatcgtctt cttcaggatt actagctata ttgtcattga gtcttcctac caatttatca
     2881 taattttcat tgatacagaa atttagattt gatttagatg tgtagtaaaa ttcatagtcg
     2941 gatagacgaa tagaatttct aaattcgtta aattgatgag agtctaattc agtggaaata
     3001 tcaaataatt gctttctaat agctttgtta ggtctaataa atagagaaat actacctaca
     3061 cctttatctg catttgaaat caattcttct aagattgttt gactttcaga atttaaagaa
     3121 atggaagcac aaatctcatt gtcaaattct tcttcgctga ttccttcaaa tgtcgcagag
     3181 ataataatcc aatgcccttg ccacccattt tgaattccat gaaaaaaatc tgattctttt
     3241 agtgttttac tactatagta ataactatca tctaataaga ttctcaatgc agtaagagca
     3301 ttggttttcc ctgaatcatt ctcacctata atagtatttg tctcattatc gaatcgaaag
     3361 atttcggatt cataatttct aaaatttcga atttgaagag tagatataaa cataagcaga
     3421 tctcctaact atttaattaa tatgattata tagtaagttt atcataaatt atgttagtaa
     3481 aagttctcat tataagggag cgtcaataat tttgtgtaaa tgattcttcc ctactgcaga
     3541 ttgtttctaa ttctcaatca gttggcttag catttgttcg agctcgcctt cggcctgttg
     3601 gaaaccttta tgactgcgac ctaggaattt ttggttgtaa gtatcaaaag acgagactaa
     3661 gaaacgttcc atcgactctt cattttggaa ctgttccttg cggtggctgt atttcttgat
     3721 ttgtttatta aaggattcaa tcaagttcgt agaataaata ctcttgcgta tggccaaggg
     3781 gaaatcatag aaagtgagca agtgatcgtt cgaaagaatc gattcaacca cttttggata
     3841 gctggttttc catttttccg caaaagtacc acgtgcttcc aacgccacct ctttagatga
     3901 ggcttgatac accattttaa aatcatcaca gacttcctta cgatcatcga cacgcacttt
     3961 atgactgata ttacgggaaa cgtgtacaca acaatgttga aaacgagctt tgggatagaa
     4021 ccgactaatc gctcctacca tcccctttaa gccatccgtg atgaacagga ggacattttt
     4081 caaaccgcgc tcttgaaggt ccaataaaat ttcctcccaa atcgtgatgg attcagtcgg
     4141 tgcaatcgca tagctcaata cttcctttga tccgtccggg cgaatgccaa ctgcgatatg
     4201 aatagcttcc ttggcgacgg ttttccgctt taacggaata tacgttgcgt ccatataaat
     4261 agcagcataa cggtcatgaa gctcccgccc attaaacgcc gttacctctt ctgtaaatga
     4321 ttttgttata ttggacatgg tttggggcgt gtagtaatgc ccatacattt tctcaatcaa
     4381 gtctgcgatt tccgacatgg taataccttt tcggaagagg tgaatgacgg tctcctctaa
     4441 cgtgtcattc gtccgtctat aagcaggaac agtctgttgc ttgaactcac cattgcggtc
     4501 gcgcggaatc tggagatgaa gttccccata ctcggtcttg accgtacggt cataggagcc
     4561 gttacgagaa ttacccgtgt taaaaccaat gcgatcatac ttttcgtaat ctaggaaagc
     4621 cgttaactcc gttttgagaa gcgtattgac tgctttctcc aaatggacac ggaataattc
     4681 attcaaatcg cctttgttcg ctagagtctt tagaatttct gtagtaaaat cgttcatagg
     4741 gaagtcctct cttctgtgaa tgtgttgtgg taactctatt ctacagaagg gacttccttt
     4801 tttctattta cacaaaatat tttacactct ccattataac aattgggtta aaaagaaaat
     4861 agatagtatt ctacctcata tttatttgaa agtaatatat aaaagacgtt caaattcgaa
     4921 cgcctctcgt taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg
     4981 attaggtgca aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt
     5041 tctgtatatt gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg
     5101 aaaacagagt gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca
     5161 ttcttcaata gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga
     5221 gactataata taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga
     5281 actgttttcc gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa
     5341 ttctaaggtt accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt
     5401 ttcattgctt cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag
     5461 tctggtgctt tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca
     5521 aaggcgaggt gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct
     5581 ccgatcttga atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt
     5641 tcgtccaagc atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa
     5701 ttggaatgtc tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta
     5761 gttccgctcc accataattt ttgatatata actgattagt atcaaactct cccccagaca
     5821 ttacataggc actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg
     5881 atgcctcgta tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg
     5941 cttccgtttt gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag
     6001 gactggtcgt tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc
     6061 ctgcttgttt aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt
     6121 ggttgtccca ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg
     6181 ttgcccaact cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc
     6241 gattattcgg tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg
     6301 tcggtgttgg ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag
     6361 tatcggcatt cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat
     6421 accgttggct gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg
     6481 gataatacga cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc
     6541 accaagtaaa tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt
     6601 taggttgagt gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa
     6661 attctccccc gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt
     6721 cattgccgat ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt
     6781 tctttgtata gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt
     6841 taggaagatt ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca
     6901 ccttaagtcc cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg
     6961 tccctgctcc ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa
     7021 catagttgac accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat
     7081 aatattttgc accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa
     7141 attcgctggc agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg
     7201 caaataaaac agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga
     7261 aattggttca gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct
     7321 gctccttttg tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca
     7381 tagtaagaat tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa
     7441 tgagaaccat aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt
     7501 tttaaagcat tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg
     7561 atttctttat ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca
     7621 tcggcagaag caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact
     7681 aagacagcat cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga
     7741 accgctgcta gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt
     7801 gctggagttg cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa
     7861 aacttgaaca ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg
     7921 ttcaaaatca tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata
     7981 aaggaagctt tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa
     8041 ggaacaacca atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc
     8101 gtcagtactt tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg
     8161 gcatagataa ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac
     8221 caaaacagcg ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta
     8281 aatagcggct gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga
     8341 atgcctaaat caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg
     8401 aaggtttgcg aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc
     8461 acacaaaact tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc
     8521 tttgtcccca tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg
     8581 aaacttacca cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag
     8641 cgacgattca tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca
     8701 acacccatcg aataattata gactttccat agccatgtgg agacagattc aggccattgg
     8761 taaccaaaaa tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct
     8821 ggcatcgctg ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt
     8881 tcaaaaaatg gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc
     8941 tatccttgat tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa
     9001 gtggtcatta aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca
     9061 gatgcttctt ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg
     9121 gcttctttca acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat
     9181 ttcaataatg tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt
     9241 aattcctctt tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt
     9301 tcactgacca acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa
     9361 tcaacataaa acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata
     9421 agcgaccaca aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat
     9481 tccaaataga ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac
     9541 tgatctttat accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg
     9601 cttttaattc gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca
     9661 gttcgaggaa ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag
     9721 gtcccttttt cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca
     9781 aaatggcatt ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat
     9841 tcaacgaaat cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca
     9901 agagcagtca aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg
     9961 atcgacaatg catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca
    10021 tatttcgtct ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa
    10081 tagttcacta cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta
    10141 aaatcatatt tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga
    10201 tagatttcat taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg
    10261 gcataggaaa caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa
    10321 cgtttagggg tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat
    10381 tcttcgaaca cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca
    10441 ggaaaaatgc ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac
    10501 aattcaatgt cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc
    10561 ccttgttcct ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct
    10621 ccttcaacct gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc
    10681 atgtacatct ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta
    10741 aacgcatcca aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac
    10801 agcaataaaa tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa
    10861 ttagattgac tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat
    10921 gttgaaaaaa gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac
    10981 cgttaatgaa atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga
    11041 tgaattagaa aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta
    11101 cactttagat caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa
    11161 agaaattgct gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc
    11221 aggtacaaca atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac
    11281 aaataattat ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat
    11341 tggcggtgat ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt
    11401 aagaagacta aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat
    11461 ctctacttac agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa
    11521 gaaatatcta ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa
    11581 tttgcatgat ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca
    11641 ctatgcacaa tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc
    11701 gtaactatga aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg
    11761 catgaagctg cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag
    11821 gatattttaa aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca
    11881 gagcatttga ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt
    11941 atgctttgct tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt
    12001 gaccagctga ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat
    12061 gttgcttttt attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc
    12121 tatgacgacg gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac
    12181 aaactgagct tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt
    12241 gctgccaaac gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa
    12301 acagtatttg aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt
    12361 tggacagagc acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa
    12421 gaaaagcaac tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa
    12481 gatacactgc tatatcctta gtgaaatata gcagtgtatc tttgactttt tcattctttt
    12541 aaccatatga cgcgttcatc tct
//
LOCUS       DACDSR010000076        12315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDSR010000076 DACDSR010000000
VERSION     DACDSR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.076"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(112..492)
                     /locus_tag="IWK15_002112"
     CDS             complement(112..492)
                     /locus_tag="IWK15_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9547682.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            574..1749
                     /locus_tag="IWK15_002113"
     CDS             574..1749
                     /locus_tag="IWK15_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9547683.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(1826..2410)
                     /locus_tag="IWK15_002114"
     CDS             complement(1826..2410)
                     /locus_tag="IWK15_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9547684.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2611..3609
                     /locus_tag="IWK15_002115"
     CDS             2611..3609
                     /locus_tag="IWK15_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9547685.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            3721..4230
                     /locus_tag="IWK15_002116"
     CDS             3721..4230
                     /locus_tag="IWK15_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9547686.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4260..4919)
                     /locus_tag="IWK15_002117"
     CDS             complement(4260..4919)
                     /locus_tag="IWK15_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9547687.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(4921..5685)
                     /locus_tag="IWK15_002118"
     CDS             complement(4921..5685)
                     /locus_tag="IWK15_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9547688.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(5693..6370)
                     /locus_tag="IWK15_002119"
     CDS             complement(5693..6370)
                     /locus_tag="IWK15_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9547689.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6407..7792)
                     /locus_tag="IWK15_002120"
     CDS             complement(6407..7792)
                     /locus_tag="IWK15_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9547690.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8002..8086)
                     /gene="ffs"
                     /locus_tag="IWK15_002121"
     ncRNA           complement(8002..8086)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWK15_002121"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8109..8624)
                     /gene="tadA"
                     /locus_tag="IWK15_002122"
     CDS             complement(8109..8624)
                     /gene="tadA"
                     /locus_tag="IWK15_002122"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9547691.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            8731..9588
                     /locus_tag="IWK15_002123"
     CDS             8731..9588
                     /locus_tag="IWK15_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9547692.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(9669..10004)
                     /locus_tag="IWK15_002124"
     CDS             complement(9669..10004)
                     /locus_tag="IWK15_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547693.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10071..11441)
                     /locus_tag="IWK15_002125"
     CDS             complement(10071..11441)
                     /locus_tag="IWK15_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9547694.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11519..12055)
                     /locus_tag="IWK15_002126"
     CDS             complement(11519..12055)
                     /locus_tag="IWK15_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9547695.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3681 a         2498 c         2072 g         4064 t
ORIGIN      
        1 tgcaacataa ttgtaccagc aactcttttg cttgccaaaa tttccacctc gatcaacttg
       61 ttctatggct tacataaaaa ttctagcaca ttttcagaaa aaatacactt tttatttctt
      121 attttctttc cgttctagtt cagataatgc ttcttctatc caaataggta actgctccgc
      181 aatagtggta aatccgatct tacgattttt attcgtgaaa tacctttttc tgcgatatgg
      241 aacagctgga tttgtttttt ctaacgttct taaagataaa ctgatttttt tggaatattc
      301 atcaatgtcg atgatctgaa ctgttactgg ctgtccgact tttaacaatg aatggatatt
      361 tttcgtataa ccggattgta cttctgaaac atgaataagt ccttgcgtct cgttatcaag
      421 agaaacgaat gcgccataag gctgtatccc tgtcactttt ccttcaatta tttgtccaat
      481 tttataagtc atctacttcc ctctcttcgc atttatcatt atactgtaaa tagctcaaaa
      541 aaagaagcga cgaaaatgac tggaggtctt aagatgatcg aatttgataa acagatttca
      601 agaaaagata cagaaagtgt aaaatgggac gcaattgctg agacatatca aatggatgac
      661 ttacttcctt tgtgggtagc agatatggac ttcttgtctc ccggtggggt agctaaagcg
      721 tttagtgaat acatcaaaca tggaatcttt ggttatgcca ctctgtctga taaactatat
      781 caggctatca ttcattggga acgccaacac catgctgtgg agttgacgaa agaaaatatc
      841 gtttttacaa gtggtgtgtt aaccagttta gctgcagcag tacaaacttt tacaaaacca
      901 ggcgattcta ttttgattca tgacccagtt tatcctccat tttcctctat tatagaaaca
      961 aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa ataatcattt catcatggac
     1021 ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat tttatgcaat
     1081 cctcacaatc ctggagggcg tgtttggagc aaagaagaat tgaaacagtt aagcgaattg
     1141 tgtttaaaac atcaagtgat tctgtttagt gacgagatcc atcaagattt aactttattc
     1201 gatcatacat ttacttccat gctaacgatt gatcctaatt tagatgatct actagttgct
     1261 tttacttccg caaccaagac tttcaatcta gctgcaatca aaaattcaat ggtatttatc
     1321 aaaaatccag agatgaaagc tgattttgaa aaacatttag tgatgaacca acagcacgaa
     1381 atcaacactt ttggactgat tggaacccaa gcggcttatg agacgggtgg agaatggtta
     1441 agccaactga ttccttatct agaaaaaaat gttcaaacag ttaatcagtt ttttgaacag
     1501 tatcttccga aagtgagaat aatgaaccct gaaggaactt acttaatgtg gttagatttt
     1561 accgcctata tttcagacga ccaagaattg gagaacactc ttgttcggaa aggaaaagtc
     1621 gtattaaatc ctggcatcac gtttggtcct tccggtcatg ggcatatgcg cttgaatatt
     1681 gcttgtccag aagaaacttt actggaagga cttaatcgaa tcaagtatgc tttagagaca
     1741 atcgattaaa tcatttattc aaaataaaag gacgagacct tactaggttt cgtccttttt
     1801 cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca atcactacat
     1861 catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct actgtgtcca
     1921 tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga gttccgccac
     1981 ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt ccaagcatat
     2041 tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg tttggacctg
     2101 aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa
     2161 aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg ccgccttgga
     2221 tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct tttttggaaa
     2281 gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga acagtaatgt
     2341 caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa tctagttgtg
     2401 ggaatgccat attgtttcct cctaatattt gcatacttca taataataat aacataatac
     2461 ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact agatacattc
     2521 tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct caattaaatt
     2581 ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac aatcatcggt
     2641 gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa
     2701 atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc cgaaaaatat
     2761 atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga tcaattagaa
     2821 aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct ctcgcttact
     2881 cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc caaagcagtc
     2941 attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga taatgctgaa
     3001 tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta tgctggaaaa
     3061 aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat gttagagcct
     3121 attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt
     3181 gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat cgatggatta
     3241 tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag tgacgagacg
     3301 attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa tttagaacat
     3361 ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc agatatgtct
     3421 acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg aaaagtgaaa
     3481 ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt acactatatt
     3541 aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa aaatcttcgt
     3601 cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca gtttcttgca
     3661 tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg gagcaataat
     3721 atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa ggaacgtggc
     3781 gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta tatttctgat
     3841 ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat
     3901 aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa attgatgagt
     3961 ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga gatcctagcc
     4021 ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta tatcgacaag
     4081 gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt ccataccttc
     4141 ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc acacaaagaa
     4201 ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt
     4261 tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca
     4321 gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa aaattcttct
     4381 ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc gttgttgaaa
     4441 aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa
     4501 acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag ccgatcattc
     4561 ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa cacagcataa
     4621 tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc agaaactggg
     4681 aaatctggca agctgaggat ggttttgaag ggattattga gataattcat taggtgatca
     4741 aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg caaatgtccg
     4801 atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag actgatcagt
     4861 gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga atttttcata
     4921 ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag gcaacccagg
     4981 tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat tattgcgtat
     5041 cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt ctttttgcag
     5101 ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga tgtagatcgg
     5161 cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag gaagaaggcc
     5221 gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac ctttttgaat
     5281 agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc ctaccactac
     5341 atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt caatcaatcc
     5401 tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa aatcgattgt
     5461 tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact catctgctag
     5521 acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa
     5581 ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg
     5641 ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc cactactctt
     5701 ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt tgttcttgtt
     5761 tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata accggttttt
     5821 tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta tggttttttt
     5881 tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg atgacaaaat
     5941 acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg attcgctgat
     6001 ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt tgttcataac
     6061 cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt tctccaagct
     6121 tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt tcacctatca
     6181 aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct gctttaggct
     6241 cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt tcctgttcag
     6301 ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt tcattttgtt
     6361 ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat ttgattgggt
     6421 agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac aaaaattcat
     6481 tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga tctacggtag
     6541 taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact tgatggttaa
     6601 tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg cgaaatccca
     6661 ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct aaaactaagc
     6721 ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc ggcaacgcag
     6781 tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac agaccactgt
     6841 ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct acttggataa
     6901 acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct acttcttttt
     6961 cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca tcttctgtaa
     7021 aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt tttttctctt
     7081 catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg gctgctattt
     7141 ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat cctaaaataa
     7201 catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg agatgactca
     7261 tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc aaaatatcat
     7321 aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga gcagtaaatg
     7381 caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg
     7441 ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca aactcagcat
     7501 gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg tttcctatcg
     7561 tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca ttcgttgctt
     7621 ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc gttgtcgttt
     7681 tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc cttggccaat
     7741 tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc atgtaccctc
     7801 tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga aacaagctct
     7861 atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg ataaatcagt
     7921 ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa aaaacagacc
     7981 cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct tagtgctgct
     8041 tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc ggcaaagaat
     8101 agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt tcgctggcgg
     8161 agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat gccaccttct
     8221 acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt
     8281 ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa aatcatcgcg
     8341 ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc caagcgccag
     8401 cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt agtagcatct
     8461 tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa tacaaccact
     8521 gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa tgcaaggcgc
     8581 atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc ttccctgcct
     8641 ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac ttttttttct
     8701 atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact tgctctttac
     8761 ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc ttttgcaatt
     8821 gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac cagccttttg
     8881 aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg ctatcttttt
     8941 aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca cgtcgaatca
     9001 aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt tcgctattct
     9061 gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat aaaccgtgat
     9121 tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga ttttgatacg
     9181 acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt ccctttgttt
     9241 gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca cagccgtact
     9301 ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa agatagtaag
     9361 ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat cattcaagaa
     9421 ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat
     9481 actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc
     9541 aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag atagattaaa
     9601 ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg ttcaaaagct
     9661 agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt
     9721 gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt gatttttgct
     9781 aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat ttctagtaat
     9841 ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg agtagtcata
     9901 tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac actggcagcg
     9961 gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat cagctccttt
    10021 ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac tcagtgaaat
    10081 ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc aaatccgaca
    10141 ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg aaatgtttct
    10201 cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat attctctatc
    10261 ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt gaaatctcct
    10321 aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa ctcaggaact
    10381 tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg aaaatcattt
    10441 gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact tttttttcgt
    10501 gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt
    10561 ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc agctacgctt
    10621 ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc aatcgttact
    10681 ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc aaacgtacaa
    10741 gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga caaaatctct
    10801 tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat tgcatttgga
    10861 tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag
    10921 cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc gttcgaaact
    10981 ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc
    11041 actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc accgggagcc
    11101 gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc aacgatcatt
    11161 gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt tccattgaca
    11221 gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct atggtaagtt
    11281 tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt tttcacgctt
    11341 ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag acgatatttt
    11401 ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt tcgttagaca
    11461 ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat gttgctgcct
    11521 aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg tttttatccc
    11581 aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc aaacttccca
    11641 atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg tgacctttga
    11701 catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat tttgcaatga
    11761 tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca atgcttaaaa
    11821 catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt tcttctaata
    11881 tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat gtagaacaac
    11941 cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct tccacgtata
    12001 ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc atcattctta
    12061 cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa aaatattatt
    12121 gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc gccttaaaaa
    12181 caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa ggtaacaaaa
    12241 aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac acagactgaa
    12301 atgactttcc actca
//
LOCUS       DACDSR010000077        12205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDSR010000077 DACDSR010000000
VERSION     DACDSR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.077"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWK15_002127"
     CDS             complement(37..216)
                     /locus_tag="IWK15_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547696.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWK15_002128"
     CDS             complement(597..1304)
                     /locus_tag="IWK15_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547697.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWK15_002129"
     CDS             complement(1297..1734)
                     /locus_tag="IWK15_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547698.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWK15_002130"
     CDS             complement(1749..2000)
                     /locus_tag="IWK15_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547699.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWK15_002131"
     CDS             complement(2000..2206)
                     /locus_tag="IWK15_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9547700.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWK15_002132"
     CDS             complement(2643..2903)
                     /locus_tag="IWK15_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547701.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWK15_002133"
     CDS             2978..3181
                     /locus_tag="IWK15_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9547702.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWK15_002134"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWK15_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9547703.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWK15_002135"
     CDS             complement(3810..4709)
                     /locus_tag="IWK15_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9547704.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWK15_002136"
     CDS             complement(4722..5315)
                     /locus_tag="IWK15_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9547705.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWK15_002137"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWK15_002137"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9547706.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IWK15_002138"
     CDS             complement(7771..9231)
                     /locus_tag="IWK15_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9547707.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IWK15_002139"
     CDS             complement(9292..11664)
                     /locus_tag="IWK15_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547708.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="IWK15_002140"
     CDS             12074..>12205
                     /locus_tag="IWK15_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9547709.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DACDSR010000078        11943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDSR010000078 DACDSR010000000
VERSION     DACDSR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.078"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWK15_002141"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWK15_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9547710.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWK15_002142"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWK15_002142"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9547711.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWK15_002143"
     CDS             complement(2169..2906)
                     /locus_tag="IWK15_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9547712.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWK15_002144"
     CDS             complement(2997..3524)
                     /locus_tag="IWK15_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9547713.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWK15_002145"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWK15_002145"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9547714.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWK15_002146"
     CDS             complement(4401..4886)
                     /locus_tag="IWK15_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9547715.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWK15_002147"
     CDS             complement(5039..5236)
                     /locus_tag="IWK15_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547716.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWK15_002148"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWK15_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9547717.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWK15_002149"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWK15_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9547718.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWK15_002150"
     CDS             9211..9423
                     /locus_tag="IWK15_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9547719.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWK15_002151"
     CDS             9425..11104
                     /locus_tag="IWK15_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9547720.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWK15_002152"
     CDS             complement(11358..11558)
                     /locus_tag="IWK15_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9547721.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="IWK15_002153"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="IWK15_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3460 a         2575 c         2075 g         3833 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tca
//
LOCUS       DACDSR010000079        11935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDSR010000079 DACDSR010000000
VERSION     DACDSR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.079"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(186..1355)
                     /locus_tag="IWK15_002154"
     CDS             complement(186..1355)
                     /locus_tag="IWK15_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9547722.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1559..2723
                     /gene="mltG"
                     /locus_tag="IWK15_002155"
                     /pseudo
     CDS             1559..2723
                     /gene="mltG"
                     /locus_tag="IWK15_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
     gene            2859..3338
                     /gene="greA"
                     /locus_tag="IWK15_002156"
     CDS             2859..3338
                     /gene="greA"
                     /locus_tag="IWK15_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9547723.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            3507..4238
                     /locus_tag="IWK15_002157"
     CDS             3507..4238
                     /locus_tag="IWK15_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9547724.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4235..5302
                     /locus_tag="IWK15_002158"
                     /pseudo
     CDS             4235..5302
                     /locus_tag="IWK15_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            5309..5941
                     /locus_tag="IWK15_002159"
     CDS             5309..5941
                     /locus_tag="IWK15_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547725.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6081..6746
                     /locus_tag="IWK15_002160"
     CDS             6081..6746
                     /locus_tag="IWK15_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9547726.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            6774..7067
                     /locus_tag="IWK15_002161"
     CDS             6774..7067
                     /locus_tag="IWK15_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9547727.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7135..8082)
                     /locus_tag="IWK15_002162"
     CDS             complement(7135..8082)
                     /locus_tag="IWK15_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9547728.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8260..9063)
                     /locus_tag="IWK15_002163"
     CDS             complement(8260..9063)
                     /locus_tag="IWK15_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9547729.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9209..10123
                     /locus_tag="IWK15_002164"
     CDS             9209..10123
                     /locus_tag="IWK15_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9547730.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10171..10641
                     /locus_tag="IWK15_002165"
     CDS             10171..10641
                     /locus_tag="IWK15_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9547731.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            10726..11334
                     /locus_tag="IWK15_002166"
     CDS             10726..11334
                     /locus_tag="IWK15_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9547732.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            11468..11668
                     /locus_tag="IWK15_002167"
     CDS             11468..11668
                     /locus_tag="IWK15_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9547733.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4004 a         1843 c         2398 g         3690 t
ORIGIN      
        1 aatcttagaa taatttggac agaacagaaa gaagaacacc tctttctgtt ctgtttattt
       61 ggaattaaag tctgtagcaa aaattgattt ttagatttgt cccacactct ttcctacgtt
      121 tagtcgagta ctttttttaa gcaaatgcta ttcaaagaag tagtatttgc tatcttttca
      181 tcatatcagc tatttgccaa cttagtagtt ccttccatca ggtcatagat ttggtttctt
      241 agatcagcag taggcagttt gatagttttg tcagctttta tattgctttc catgatgata
      301 gcatcctgtg aatctttgct aaatatagca gatgtatcaa aagagtcgat atttccttgc
      361 gacaggatat aattatctcc gctatatacc ccaccactat atggatgtcc attcgcattt
      421 tcacgccata atgagaagag taagtctttg gaaactagtt ttccagataa aacttgtgta
      481 aacagcgtat ttaaatcata gacggacata ctgatattac cagtccctaa ttcgttatgg
      541 atctctgttt cagattctgt cagtggttta aagtaatcat cctctttaga catttgatag
      601 gatagtgcat gattggatga atttttgact tggtcataaa attttgtatg ctgtaatcct
      661 aaaggttcga tgatatcatc ggtcaataat ttttcatacg gttgatttgt taatttgatc
      721 aaaattccag tcaatatggt aaagttcaat gaatcataat gagaggtctg ataatctgta
      781 aattttaagt gctgaatcgc aaattgaacg acttcttcat cagtcatcgg tatggttgga
      841 gaagcagttc ggtgcaagcc tgagcgcata tacagcaagt cacggatagt aatttgctgg
      901 ctgccagcaa tttgtggata gaattgagcc agcttagtat ctaaagaaag gtgcccacgc
      961 tcgacttgtt ttaaaatcaa aagagtagtc aatgcttttt gaaccgaacc aatctgataa
     1021 taaagagaag gatcatttgg ttgttgcgtg agtttattcg catagccata gcctttttcc
     1081 atgatgatgc gattattttt ctttacgtaa attgagccaa tataatcatt ttttcttaat
     1141 agttgatcga ttttttcaac gagctgttgt tcattggtag tcagtatcaa ctttttttga
     1201 gctgattgag catgggcaac tatctgagag tgttcattat tgtaaaattg ccatccaaga
     1261 atagttcctg tagagaacgc actaaggcta aacaacacga caacgatcat taaaaatgaa
     1321 tattttcttt ttttagcatg ttttcctttt ttcataatag tttatttagt ctcctttctt
     1381 ttttacagaa agaataaagc taaaatagct tgtatcttga taacatttat tttaatctta
     1441 tagtaatgga tttactactt acatttttga tagaatattt aaataaaatc attcacgcca
     1501 attcaaatat catgtataat gaataaagtt acgaacttga tagtaaggag gagttgtttt
     1561 gtcaggaaac aaacaacacc caagtaaaga taaaaaagaa gccagtaagg cagctacagc
     1621 agatataatc gacaagcgga gaaatctgcg taaaaaagaa gataagattg tgaggaagat
     1681 cattttagtg attgctctta cattattgat tattggcgga tttcttggat ttaccgtgta
     1741 tcgttatgta gatagcgggt tgaagccttt agataaatca gatgaccaat tagtccaagt
     1801 cgagatcccg agtggctctt ctaacaagca gattggtgag attttggaga aggacaatat
     1861 tattaaaagc gggattgttt tcaattatta tacaaaattt aaaaatttaa ctggctttca
     1921 agctggctat tatcagttag cgcctaatat gactcttgac gagattggca aacagttgca
     1981 agaaggcggt acttctgagc caacaaaagt tgcagatggg aaaattgcca ttccagaagg
     2041 gtacgatatc gatcagattg ctgaacgggt agcgaaggta actggtaaag acaagaaaga
     2101 atttttagat ttagtgaatg atgagacttt ctttaacgag ttgcaccaaa aattcccaga
     2161 actgttggaa agtgcatcta aagctgagaa cacaaaatac cgtttggaag gttacttatt
     2221 cccagccaca tatgattatt acacgagtac ttcgcttaag gatctagtga ttgaaatggt
     2281 gaacaagacg aatacggtca tgcaaaacta ttattcagct atcaagcaga aaaatttgac
     2341 tgtacaagaa gttttaactc tagcttctct agttgaaaaa gaaggcgtaa aagaaaatga
     2401 tcgtaaaaac attgcacaag tcttctttaa ccgaatcaaa gcaaatatgc cacttcagtc
     2461 agatatctct gttttatacg cattaggtga acacaaagaa ttagtgacgt atgaagatac
     2521 agctgtagat tcaccatatc aataccggct atggacctgg accttttgac aatccaagcg
     2581 aagaggcaat caaggcagta ttagaaccag cagaaaatga ctattattat ttcgtagcag
     2641 atacgagcac tggaaatgtt tatttcgcta aaacttatga agaacatatg gaattagtgg
     2701 aaaaatatgt aaataattct tagaaaaaca ggctctagac attgatcatt tttagaaaaa
     2761 catttacatt tgaagaaaag cgtggtaatc ttttttggac tatattcctc aaaagattac
     2821 cttttgcggt attttatcga aaaaaggaga cgttacacat ggtagagaaa gtttatccaa
     2881 tgactctaga aggtaaagaa aaattagaaa aagaattaga agaactaaaa acagtcaaac
     2941 gcggagaaat cattgaacga atcaaaattg cccgcggttt cggagatctt tccgaaaact
     3001 ctgaatatga atcagcgaaa gatgaacaag catttgttga aggaagaatc acaacaattg
     3061 aaaatatgat tcgttttgct caaatcatcg ataacgacgg tgtagatgca gatgaggtat
     3121 caattggtaa aacagttaca ttcatcgaac taccagatgg agaagaagaa gagtacacga
     3181 ttgtcggaag cgctgaagca gatccgtttt ctggcaaaat ttccaatgat tctcctattg
     3241 cgcaagcatt gattggcaaa cgtaaaaatg atgaagtaac tattgctact ccaggtggcg
     3301 atatgttagt taaaatcgtc aaagtagaac aagcgtagtc tgactgaaag atctagagag
     3361 ttcatcttac tgatgataca ctctagatct ttttctcttt gtgtatttta gatttcaaca
     3421 aacgatctta catctaaagt cctatagcca acagttaaaa aaataggtat gatgaaatga
     3481 gaatgtgtca ggagggagaa agagcgatga atagttcttg gcgttttttt gtggtagttg
     3541 aagcactact gctgttattt gctgtttggc aaatcgtaaa caatactgga cttttgcttt
     3601 tagtgatatt cggtattttc aatatatatc tagctatgcg caaacatcct cgtactaaat
     3661 ttcaaaattt tcaattgata ttaggaagtt tggtcatttt ttttagctta gtcaacagtc
     3721 ctgctttatg gatgatggct gtatttgccg ttttatttat tggtctgaaa ggtgtagaga
     3781 tttcagggat agatttaacc aaaaatgctt tttggcgaaa gaaacagatc atgatgatcc
     3841 aaacagagca attaaaaaca cataataatg agcggaaaag acagcaatta ttcggtaacc
     3901 aacgtattgg aaacgacgtc tatgaatggg acgatatcaa catcgccgtc atttctggtg
     3961 atactatcat agatttgggc aatacgcttt tgccaaaaga tgataatatt gtgattgtaa
     4021 gaaaagggat aggcagaaca cggatcttag tacctttggg cgtagccatc cgattagagc
     4081 atgcgacatt agtaggaaat gtcttgtttg aagaagagca attttctttg aaaaatgaac
     4141 aaatcaaaat ctacagcagt gattatgatg aaaaccctcg tagattgaag attattacta
     4201 ataccttgat tggagatgtc gaggtgatac gaatatgatg ggaaaaatat ccagagcgat
     4261 gctagctgtt tattcgggga ttgcggcttt ccttattatt ctattttcac ttttcactta
     4321 tttttatgcc agcaatcaaa gtcattggtg gggagaatta ctgcgtgcac gtttattata
     4381 tgttccgctt atcttccatt agttagccat ttccttaggt gtaggattaa ttgtctttct
     4441 attattatca cttattcaaa agacaaaata cgggaaaatc gaagaaaagc tgcgtgcact
     4501 ttcttctggc aattatgaat ccaaattgtt gcttcttccg atcccaagtg catcggacga
     4561 tttatacatc aaagatattg ataaggaaat cactaagata aaagaaaaaa tgattgaagt
     4621 atctagtgaa ttacaaattg taacgagccg tccccaatat gtagatggac aaaccaaaga
     4681 agaaatttta gaattagaaa gacatcgatt agcccgtgag ctgcatgatt cagtttcgca
     4741 acaattgttt gcagcaatga tgatgatgtc ggcattgacg gagcaagcag aaaaaagcga
     4801 gacaccagaa atgtttcgca agcagttgaa aatggtagca gagatcatca acgcttccca
     4861 gtctgagatg cgtgcgctgc ttcttcatct acgtccagtc aatttagaag agaaaagttt
     4921 aaagcaaggt atcgagcagt tattgaagga attgcaaaat aaaatccaga tttcgctgaa
     4981 atgggatgta gaagatgtaa aactatctag ttccattgag gatcacttgt tccgaatcgt
     5041 tcaagaattg ttatcaaata cattaagaca tgctaaagcc aatgaattag aggtatattt
     5101 gcacaaaata gacaataatc ttttgttacg tattattgac gacggaacag ggtttaatat
     5161 gaatgaaacg aaaacgggaa gttatggatt gaacaatatc aaagaacgag tagctggtat
     5221 tggtggaaca gtaaaaatca ttagttttaa aggtcaaggt acaagtgtag aaatcaaggt
     5281 ccctttgatg aaggaggcat agataaggat gataaaagtt ttattagtag atgaccatga
     5341 aatggtgcgc ttaggcgtct cttcttattt atctattcaa gaagacattg aagtaattgg
     5401 agaagctgaa aatggacgac aaggctatga gaaggcgatg acacttcgac cggacgtcat
     5461 tttaatggac ttagtcatgg aagaaatgga tggtatcgaa tcaacaaaag caatcttaaa
     5521 agattggcca gaagctaaaa tcattatcgt aaccagtttt attgatgacg aaaaagttta
     5581 tcctgccatt gaagcaggtg cagcagggta tttgttgaag acctctaccg cacatgaaat
     5641 tgctgatgct ataagggcaa cgcaacgtgg cgagcgagta ttagaaccag aagtgacaac
     5701 aaaaatgatg gaaaaaatga gtcggcgaaa tgagcctgta ttacatgaag aactaacgaa
     5761 tcgggaaaat gaaattttaa tgctgatttc tgaaggaaaa agtaatcagg aaatagcgga
     5821 cgaattgttt attacactga aaacagtaaa aacacatgtt tccaatatat tggctaagct
     5881 agaagtggaa gaccgtacac aagccgctat ctatgcattc aaacatggtt tggtgaaata
     5941 aaaacgatga taaagaggat aatcaaaagc tgagaaaata actattattt ttcttggctt
     6001 ttttacaata atgatgcttg cttttgtttt tgtaaaggct ttgttatact gagaaaagaa
     6061 gatactaaag gaggaaaacg gtgaaacaaa attttgcaat tgtaggtctt ggacgatttg
     6121 gcggaagtat ctgccgaaca ctgatcgaag ctggtcaaga agtactggct attgatagta
     6181 atgaagatcg ggtcaatgaa tatatgaata tagccactca tgctgttgtg gcaaatgcac
     6241 aggatgaaat gacacttcga tcattaggca taagaaattt cgatcatgta attgtcgcta
     6301 ttggagaaga tattcaagcc agtatcttag ttactctgat ggtaaaggaa atgggcgtac
     6361 caaatatttt agcaaaagcc caaaatgagt atcatgccag agtacttgaa aagatcggag
     6421 ctgatcgcgt cgtccatccc gagcgagaca tgggaataag gattgcgcat aatttagtgt
     6481 caaagaatat tttagattac ctagagttgt cagataaata ttctttagca gaaatccgag
     6541 tctccaatcc taaatttttc aacaaaacat tagctgaatt aaatttcagg caacgttttg
     6601 acttaacagt agtcgccata cgaagagtag atcaaacggt tatcgcttct cctgcagcag
     6661 atgaatatgt tcgtgaaaat gataatctac tggttattgg agagacaggc gatgtggata
     6721 ttctagacga taagatgaat caatagaaag aaaagagaaa gtaggttgtt gggatgaaat
     6781 taacggtaac gccaaaagca caaaagtggt ttgactcaga aattgatctg ccagaaactt
     6841 acggaatacg tttttttgga aaagtctacg gcaaaacaga ggttcatgat ggtttttcga
     6901 ttggaatgtc agttgaacag ccagaacatc ctgtaaagaa agtggaaatg gatgggacac
     6961 ttttttttat cgaggaagca gatgaatggt tctttaaagg atatgattta cttgttgatt
     7021 atgatcccaa cctggaggag ccaacctatc actttaaaaa acaataaaaa aagagagggt
     7081 gacacaattt ttgtcacctt ctcttttttc ttttgaaaat attgttttca gctattaata
     7141 tcctgcagac aattttactt gattggtttg aagctcatgg gtggcaaagt agcttttcag
     7201 attggttaaa aaaatagcca ttaatttctt ttgaaaatca ggtgtcaaac ctgcaatgtg
     7261 ggaagtaatc agtacattgt ccattttcca aagaggatga tcttgtggaa gaggctcttc
     7321 ctcaaaaacg tctaatgctg caaaggaaag ttgctggtta tttaaagcct gcaccagatc
     7381 ttgtgtctta acagaagcac cacggccaac attgatgaat acagattttg gatcaaagtg
     7441 ttcaaacata tgctgatcaa agagcccagt tgtttcctcc gttagaggta agatattgac
     7501 tacgaaatct acttcaggaa gaacttcctt tagttgattt aaagaaaaag tcttttccat
     7561 tccttctgct ggatgaccgg atgtattgat tccatagcag ttcatgtcta atgctttgca
     7621 tttttttgca atcatttttc caatatggcc agttccaatg attagtattt ttttcccttg
     7681 gatagattgg taatggaccg atgaacctaa ccatttcatg tcactctgtg ccttttgtgc
     7741 ttgaaaaaga ccacgagaat acccaaggat gattcctaag acatgctccg taatcgattc
     7801 actgtggatg ccactaccat ttgaaagtaa aatatgtttc tttgaaaaag tctcaagggg
     7861 tagctgatca acaccagctg agatagattg gacccatttc aaggtagaag aggctaatag
     7921 tttttcttgg tagtctgagt tccagcccaa actgatctct acattttgta gatcctctgg
     7981 ttgtaattct gttttaaaga tataaccagg cgctatagta tggatttctt cgataaattc
     8041 atcacgaaat ggacgatctg cataaatgat tctttctgac atagttaatt tcccgccttt
     8101 ctttactcta agtgtagcgc atacaagaag aactttgaag cgccttgctt gttttttgag
     8161 aaagaaatat ttttcaaata aaaaaggggc tgtgacaaaa aaatgtcaca acctctaaaa
     8221 gggtctattg aagtagaaat aacgagttgt ctttatttct taattgatcg atatttttgc
     8281 ccatcctttg acagcttgag caaaaataaa gattcctgca aaagcaatga aggcgctagt
     8341 gtcaaaagaa ggaagaaaga ccatcaagat tgttgcaatt atagtaggca atgaaattga
     8401 atagatcaat gtttttaaac aatcaagaaa agtagctttt gttagtcgaa agcgtgcata
     8461 gagaaaagca gcaatgttgg taaatagcaa agtgatcagc agattcagaa aactaggata
     8521 aatagaaaat ataaatgtca ggacttttat ccaaagtggt atagatgctt cacttaaaaa
     8581 agaacggagc tgcttacctg ttaagttttt taaagattca ttggtgtagg gaagttctaa
     8641 ttggttattg ttcaacaaag cagaagtcgt gcctgtatta ggaagagcta tgataagttt
     8701 gttttttaac atcccgacac taacaaaatt ccccatcaaa tcagtagaga tgtcctgttc
     8761 tgttcgtttt ccctctggat caaatgtgaa gataatggag tttgtttggt aaatgaatcc
     8821 ttctttttct tcggtttgaa gctgcccgtt ttggattgta aaatcaggaa tgcgagtagc
     8881 aatcttttgc ccatccaatt gaatgttgtt taagacttga aaaacttgaa aagagattgg
     8941 caaagccata attatactca aaactaaaag atagacaatg gctttccaaa atgggacatt
     9001 tctcgcctcc tttaggtctt tgaatcgtat aaaggaactg ataattaatt gttttgttgt
     9061 cataagtact cctcatctag tagtgtcatc acttgcaatt ctagcgaagt atcaagtgaa
     9121 atacaagtgc tttaaggaaa cagctgaatg attggtgaaa tatcaaaaaa atgttattct
     9181 tgtctaaaga ctatccaata taaataaaat ggaggaagga atagtgccta cacagccaca
     9241 gagatcatca aaaaaactat ttggagtagt ggttttgaat gcggtgatca ctttatcaga
     9301 atttattgga gggattatgt cagggagttt gtctcttgtt tcagatgctt tccataacct
     9361 tagtgataca ttggcaatta ttttttctta cggggcacaa aaaatggccc gaaaagaagc
     9421 aaataaaaaa cgtacgtatg gctaccagcg tttagaaata ttgagtgctt tcatcaactc
     9481 attcatattg atcatattat ccttgttttt aaccgttgaa gcattcaagc ggttcacttc
     9541 accagaaaag ataaatagtc atttgatgct aacagtagca gttattggat tgctcgcgaa
     9601 tttgttctca gtattattat tacgacaaga agcagatgaa agtttgaata taaaatctag
     9661 ttatttgcat ttgttaagtg atacactgtc ttcggtaagt gtgattatcg gagcaatttt
     9721 gatccgtttt ttcgatatct actggatcga tccagttatt acactgatca tttcgatata
     9781 tatattgata gaagcgataa tggtgatcaa aaaagctgcc gctattttga tacaaagtgc
     9841 acctgctatc gactatgaaa aaatggaaca agaaattgaa gcaatcgatg gggtaaagga
     9901 tgttcatcat gtccatattt ggcagtatag cgaaaagata attattttcg acggacatat
     9961 tgattttgaa gatcagctac taagcgagat tgaaaagata tatcctcgta taatttcttt
    10021 attaaaatta aaatatggta ttacacatgt gacgatccaa gcagagacaa aggcaaagga
    10081 tcaaaaaaaa ttaatttttt taaataaaga tgaaagttat tgaaatatag agtatgataa
    10141 ataagaattt ttaataaggt aggttggctc atgaagcagt atcgacagtt aaaaggatat
    10201 ttagaagcaa aaggatattg ggagatacta gtctatctct tttttggtgg tttggctacg
    10261 attgtaaatt ttgttagttt tgctctcgca cgccaatatt ttgatttaag tatggcctta
    10321 tccaattcta tttcgtggtt ttgttctgta ttgtttgctt ttgtaacgaa caaactttgg
    10381 gtattccatt caaaatcacc gaattttaca catgcactta ttgaatgcgg taagttcttc
    10441 ttctaccgta tcctttctta cggattggac atgggggcaa tggtattgtt gatcaatgtt
    10501 atgaacagca atgagtatgt ggcgaaaatc atcactcaaa ttattgttat ccttgcgaac
    10561 tatattttca gcaagttgtt tatttttaaa gaaactgagg tttttgaaga agaagtaggt
    10621 caaagtgaaa aagacgacta ggcataacaa ttgaagaatt tccttctctt tgttactctt
    10681 tgtaatgtag acattattaa gaaaacattg aggaggaatt tttttatgac ttacacatta
    10741 ccagatctac cttatgcata tgacgcattg gaaccttata ttgacgaaga aacgatgcat
    10801 ctgcatcatg ataagcatca caatacttat gtgacgaatt taaattcagc aattgagaaa
    10861 tacccagaat taggcgaaaa aacaatagaa gaattattat ctgatatgga cgctattcca
    10921 acagatatca agacagctgt acgtaacaat ggtggcggac atgctaacca ttcatttttc
    10981 tgggaaatca tggcaccaaa tgctggtggc gaacctacag gagaaataaa agaagcgatt
    11041 aatgaagctt ttggtgattt ttcttctttt aaagaagaat tcaaaaaagc agccgctgga
    11101 cgatttggtt ctggatgggc ttggcttgta atggaaaatg gaaaattagc tattacctct
    11161 actgcaaatc aagattctcc attgatggaa ggaaagacac caattctagg tttggacgtt
    11221 tgggaacatg cttattattt gaaatataaa aatgtccgac cagattatat tgcagcattc
    11281 tggaatgtta ttaattggga tgaagtaaac aaacgtttcg aagcagcaaa ataaccattt
    11341 cctccgtcat atcagtctta aaaataattt ggaaaataaa agagcgaaag cttgctagcg
    11401 ttttctaaat tgggtataat gttcttgcaa ggttacttgc aagaactttt ttggaggata
    11461 tacatttatg gaacacggaa cggttaaatg gttcaacaat gaaaaaggat ttgggtttat
    11521 tacagtagac ggcggagatg acgtatttgt tcatttctca gcaattcaag gagacggctt
    11581 caagagccta gaagaaggtc aagaagtaga attcacgatt gttgaaggtg cacgcggacc
    11641 tcaagctgct gaagtgacta aattataata atttgttgaa taaagacacg acttcttaga
    11701 gagtcgtgtc tttttgtcta tttatgaagt ttttagattt tttggctaag taaatgcatt
    11761 tgttcacttg acatgtccta actaattatc aagcaaggaa cattttcttc gttagtaatg
    11821 gattttaaag aaatttcagt ttttggatgt tgaataaaag accatttgtg aaaccactag
    11881 tatacatact cattaccatg tcaattctat tactttaaaa gaaaatcgtt ttact
//
LOCUS       DACDSR010000080        11864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDSR010000080 DACDSR010000000
VERSION     DACDSR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.080"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..128)
                     /locus_tag="IWK15_002168"
     CDS             complement(<1..128)
                     /locus_tag="IWK15_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9547734.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNT"
     gene            complement(359..1489)
                     /locus_tag="IWK15_002169"
     CDS             complement(359..1489)
                     /locus_tag="IWK15_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9547735.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(2030..2236)
                     /locus_tag="IWK15_002170"
     CDS             complement(2030..2236)
                     /locus_tag="IWK15_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547736.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2252..2866)
                     /locus_tag="IWK15_002171"
     CDS             complement(2252..2866)
                     /locus_tag="IWK15_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9547737.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3013..3696)
                     /locus_tag="IWK15_002172"
     CDS             complement(3013..3696)
                     /locus_tag="IWK15_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9547738.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3805..4263)
                     /locus_tag="IWK15_002173"
     CDS             complement(3805..4263)
                     /locus_tag="IWK15_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9547739.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4347..6110)
                     /locus_tag="IWK15_002174"
     CDS             complement(4347..6110)
                     /locus_tag="IWK15_002174"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9547740.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6331..7791)
                     /locus_tag="IWK15_002175"
     CDS             complement(6331..7791)
                     /locus_tag="IWK15_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547741.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7999..8925)
                     /gene="glsA"
                     /locus_tag="IWK15_002176"
     CDS             complement(7999..8925)
                     /gene="glsA"
                     /locus_tag="IWK15_002176"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9547742.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8948..10375)
                     /locus_tag="IWK15_002177"
     CDS             complement(8948..10375)
                     /locus_tag="IWK15_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9547743.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10406..11620)
                     /locus_tag="IWK15_002178"
     CDS             complement(10406..11620)
                     /locus_tag="IWK15_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9547744.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3589 a         2439 c         1956 g         3880 t
ORIGIN      
        1 tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat gttagaaaca
       61 gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt atattttgat
      121 gaacgcatat gtagtcctca tttcttggga gttttgagac tactatatgc gtttttttgt
      181 tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc accacgggta
      241 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
      301 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      361 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      421 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      481 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      541 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      601 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      661 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      721 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      781 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      841 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      901 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      961 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
     1021 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
     1081 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
     1141 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
     1201 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1261 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1321 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1381 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1441 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1501 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1561 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1621 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1681 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1741 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1801 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1861 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1921 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1981 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     2041 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     2101 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     2161 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     2221 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2281 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2341 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2401 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2461 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2521 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2581 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2641 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2701 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2761 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2821 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2881 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2941 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     3001 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     3061 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     3121 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     3181 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3241 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3301 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3361 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3421 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3481 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3541 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3601 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3661 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3721 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3781 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3841 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3901 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3961 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     4021 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     4081 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     4141 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     4201 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4261 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4321 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4381 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4441 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4501 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4561 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4621 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4681 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4741 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4801 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4861 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4921 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4981 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     5041 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     5101 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     5161 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     5221 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5281 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5341 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5401 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5461 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5521 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5581 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5641 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5701 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5761 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5821 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5881 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5941 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     6001 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     6061 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     6121 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     6181 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6241 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6301 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6361 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6421 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6481 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6541 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6601 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6661 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6721 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6781 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6841 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6901 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6961 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     7021 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     7081 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     7141 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
     7201 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7261 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7321 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7381 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7441 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7501 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7561 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7621 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7681 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7741 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7801 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7861 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7921 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7981 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     8041 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     8101 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     8161 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     8221 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8281 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8341 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8401 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8461 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8521 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8581 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8641 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8701 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8761 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8821 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8881 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8941 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     9001 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     9061 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     9121 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     9181 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9241 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9301 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9361 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9421 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9481 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9541 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9601 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9661 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9721 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9781 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9841 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9901 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9961 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
    10021 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
    10081 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
    10141 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
    10201 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10261 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10321 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10381 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10441 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10501 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10561 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10621 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10681 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10741 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10801 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10861 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10921 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10981 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    11041 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    11101 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    11161 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    11221 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11281 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11341 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11401 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11461 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11521 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11581 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11641 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    11701 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    11761 tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg
    11821 tcttttttta tttttaaaga tattttttca taaggaataa agaa
//
LOCUS       DACDSR010000081        11649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDSR010000081 DACDSR010000000
VERSION     DACDSR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.081"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(126..1589)
                     /locus_tag="IWK15_002179"
     CDS             complement(126..1589)
                     /locus_tag="IWK15_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9547745.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1653..4670)
                     /locus_tag="IWK15_002180"
     CDS             complement(1653..4670)
                     /locus_tag="IWK15_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9547746.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4821..5660)
                     /locus_tag="IWK15_002181"
     CDS             complement(4821..5660)
                     /locus_tag="IWK15_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9547747.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5805..6050
                     /locus_tag="IWK15_002182"
     CDS             5805..6050
                     /locus_tag="IWK15_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547748.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6113..6535
                     /locus_tag="IWK15_002183"
     CDS             6113..6535
                     /locus_tag="IWK15_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547749.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6629..6946)
                     /locus_tag="IWK15_002184"
     CDS             complement(6629..6946)
                     /locus_tag="IWK15_002184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547750.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6973..7179)
                     /locus_tag="IWK15_002185"
     CDS             complement(6973..7179)
                     /locus_tag="IWK15_002185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547751.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7160..7342)
                     /locus_tag="IWK15_002186"
     CDS             complement(7160..7342)
                     /locus_tag="IWK15_002186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547752.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7356..7706)
                     /locus_tag="IWK15_002187"
     CDS             complement(7356..7706)
                     /locus_tag="IWK15_002187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547753.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7732..8034)
                     /locus_tag="IWK15_002188"
     CDS             complement(7732..8034)
                     /locus_tag="IWK15_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547754.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8037..8270)
                     /locus_tag="IWK15_002189"
     CDS             complement(8037..8270)
                     /locus_tag="IWK15_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547755.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9256..9578)
                     /locus_tag="IWK15_002190"
                     /pseudo
     CDS             complement(9256..9578)
                     /locus_tag="IWK15_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9701..10816)
                     /locus_tag="IWK15_002191"
     CDS             complement(9701..10816)
                     /locus_tag="IWK15_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547756.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10822..11220)
                     /locus_tag="IWK15_002192"
     CDS             complement(10822..11220)
                     /locus_tag="IWK15_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547757.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3090 a         2384 c         1778 g         4397 t
ORIGIN      
        1 aactacgttc tagatagata cgagatttca aattaaaaaa cgaaaatgag agatacaaat
       61 atatcaaata tttaaaaagt aaccacattt tctataccac tccttatcag aaacagcctt
      121 attctttaga ataagactgt ttctgggttg attaatttgt cttttgttaa actaccatct
      181 aaggcaagtt ctaaatgtaa atgtggtcct gtactatttc ctgttgaacc cacataacca
      241 ataatttgtc cttgttcaac tgtttgtcct acgttcacgc aatattcctg ttggtgcgca
      301 taaagagcca cacttccgtc ttcatgctca attgtgacat aatttcctga caaagtatga
      361 tacgaagctt ctatgaccgt tcctgccttg ctcgcataga ttggttctcc ttgattggct
      421 gccatgtcta atcctctatg aaattcttct cctcgccaac cgaaggaact agaaattgaa
      481 taatttttta gaggtaccat atagccttct ttgcttagtt gtggaccagc tacttcttta
      541 tcatatttga ttaagtcata ggtttcgatt aacccattta attttttatc gtagctagtg
      601 tctgttgcgt atcttcctgt taaaaattta gtcgcttcct gataggtttt tgccttactt
      661 ttccaaacac catcataaaa agaggtattt ccaactaagc cttcttttaa cagtttggca
      721 tagtctttta agctttcttc atagctctca tattgccgaa aagttgcttg ggtcgtataa
      781 agtgtcccat tgcctaaatc ttcttgtgtc gcaaaagaga cgccctttcc gttatgtgtg
      841 cctttgattc caaatagatt gtagtttggt gcctgagcca attgactttg tccgcttgcg
      901 gattctaaaa tggcttgcgc gatcataaca gaagcgtata aatcattttc ttgtccaatt
      961 ttacgagcag attcaccgat tttacggata aacgtttcta cagacgcgtc tttttcaaaa
     1021 tgaatagtcc cttcgttatc ttgtgtgctg gaagggacta attcttgtat cggtgcgctt
     1081 ggtgcttggt ggtagttttt tgtctcttct gaaccgattg attgtgcctg cggtacgtca
     1141 ttcataggta cagtcacact ctgttcaggt gccttttgtg ggcgttccgt ttttgttggc
     1201 ggttccgctt ctgtttcggt acttggtttt tgaggagtct gtgagctttc atcagaaggt
     1261 tgctcatttg gttcttctga cggttcctcc tcaggttgtt gattgtcttc tggtttgtct
     1321 ggttgattcg gtgtttctgg ttgttcttca gaacctgttg gtggagcgtt catttctgga
     1381 ttatctggtg tatctattgt atcttcagtc ggtgtttctg gttgattttc ttctccagtg
     1441 gaatttgttg tcgtttcttc agtgatcgtt gcagattgcg aatttgtcag ttgattgttg
     1501 ccttgttcat tagccaaaac cgctggcgta tacccaaata cttgacctac taataacaaa
     1561 gcagataaac atttactcgt ttttttcaca agattgcctc cattcttcaa tcaatcatct
     1621 agagacagct gattgctgtc tctagtcata ctttaattgc tttgtttcat tttacgacga
     1681 accagaacag cccccacacc aacaaaaaga aacagtccca atgcaattaa cacatcgttc
     1741 actgtttctc ctgtttttgg taagctttgt tttggtgtgc tcgtcggcgt ttgtttgaca
     1801 ggggttgtcg gcgtttcttt tttaatgcct tttgctgtca atgtttgttt ttcattcttc
     1861 aaatcaaagt gacgtgcttc ttcctcttta tggtctttat ccttatagac aatatgagtt
     1921 gcgaccaatt tctccccgtc ttttaacgtg cctgctttgt agtcaaagga tactttaaac
     1981 tcttcagacg ctttagaaac tttacgtgca gtttctaccg taccaacgac tgtatctttt
     2041 ccatttttat caatcttatg gaattgagtc acccagtatt tggtttcccc taccgtagat
     2101 tctggaaatt cttgcgatac atgatcgatc aactcttggt ctttgtctgg atcaaatgtt
     2161 tggtcgccat tcactgtaat aaagcgtgtt tcgatttttg gttctttcgc agtcactgtt
     2221 tgatctttat tggttaaatc aaaatgacga gcgaattctt cttcctcttc tttgtcgtta
     2281 tagacaatgt gcgttgcgac aatcgtgtca ccgtctgaaa ccatgtctgc tttgtagttg
     2341 aacgtcacat caaatgtttc tttttcatcg gtcacttttc gtgtttcttt taccgtatct
     2401 aatacagtga cttttccatc ggcagtaatt ttatggaatt gcgtcaccca atatttctct
     2461 gtcccaaccg cagatttagg gaatacttgg gacactttat ccactaattc ttggtctttt
     2521 ttcgggttga aatcttttcc acctgtcgtt gtcacaaaca aagtagaaat ttctggttgg
     2581 tcttcttctg gaaccacgat ttcttcgttt ttcacagtaa ttggttgtac ttctccgtct
     2641 ttgaccgtta ctaaaatcgt ttctgtttgt cctgacgctt gcccataatg ctcagggaac
     2701 acaatttccg taaatttata cgtaccgtct ggtaatttta gtttttctgg ttcttctttt
     2761 gtttcataca caaaggacac atttttatta tgtgcagtca taccttcaat caggaatttc
     2821 gctcctggta attgtagatg attgattgaa tcgactttat tgaagtctac ttcacccgtt
     2881 tttaacaagt tgtcaatcac atctaattca aacgtaagca caggcgtcgt gttatcgcca
     2941 taggccacag agaacgtggt ttgttcttca gaaggttgac ggtagttgtc tggcacattt
     3001 ttttcaatca attggtattt gttttgatcg ccattgtaga attttttgtt tgccttccag
     3061 tgtccgtctt tgtcagtttt tactgaatcc aagacttctc cttttttcac aaggactgtt
     3121 tctttatctg gcgcagtaat atctgttttg gcgataatat caaattcgat attttctgca
     3181 ggaacggtat cataaacagg tttgccttga ttccaggatt cttccgcatt atagacttct
     3241 ttgactttct ttccttctgg aatgaactgt tgtaagtcat tttttgcagt taacgtcact
     3301 tctttgtttg gttcaatcgt aatcgtatga gtttgtttgt ctagaatata cccagctggt
     3361 gctttgactt ctttgacatc caatttattt tgaacatatt ttccgtcttt ttcatagaat
     3421 gcagggattt cttttaaggc gatttcccct ttggcatccg ttgtatattg ttttgtttct
     3481 cccgtatcat gacgtttgat ttcatacgtc acatttggaa cttttttccc tgtgacagcg
     3541 tctagtttgg ttagtttgaa gtcgccattt tttagaactt taatagttag tccagctcta
     3601 ttaggatctg gcacataaag ctttcctaga ttttgagcaa caggatctac ataggcaaaa
     3661 cttgaaccta cccctctaga atttggatcc agtgaagaaa cagaaattcg tccgtcattt
     3721 gagttttgat ttgcttttat aaacaaacta tttcctgatt ttcttacttg aacgttagct
     3781 gtgttttcaa caaccgaccc atcccattga gaaaacacac cttttgtatc cttaacttct
     3841 acttcttccc ctactttaac cgtcactgtt tggctatcaa aagaaggatc ttcaagtaaa
     3901 ttgtttattt ttctatctac agtctgtttg aaagcttcat aattagattg tccaatagtt
     3961 ccataattaa caaatgaacc acctaaaact tcccaaatat atgtttgtgc taatacatag
     4021 ttatccattg aaccactaga atagtagcca aaataaccaa ttaaagaaat cttttttcta
     4081 atagcacttg tcgtaattaa attttcgagt ggtgtcacat catattgaat acccgcactc
     4141 gtgattgcgc cttcttgaat gcaaaaaaca tcttctccgt caatttgcca gcgaaccatg
     4201 taatcatgag tatacattgt tccttcagga gtaattcgag tcatattcac atctttacga
     4261 ccattttcag tcaatgttag attggtagca cgtgtctgtg cattcttttc caagattgct
     4321 tgccattctt cagattcttg cgccaatcgt tcttgttcag aaaaagaagt cgctttttga
     4381 atatcttcaa tggttataaa gtatttctca taaccttcag ggacctcttg agtcattaca
     4441 ttgggcggag aaacatcttc tgtcgtgtcc tgcgtagttg tctcttctgt tgtttcactt
     4501 gtggtttcag tactgatcgc ttcttcacta ctttttgatt cttcagtctt cattacttct
     4561 tcagtagaat ttgattcatt tatcgtttct gcaaatgccg ttacagatcc taacatggga
     4621 ttgagaaatg taacgaaaat tgccaaccaa gttgccattc ttttaaacat atttttttcg
     4681 ttcctttcat tttatttttc agtcttctaa agtaatttct tatggacttc gacaccgaaa
     4741 catttttatc ctcctcaaaa caaaaaaaga ctagaactta taattctagt cttcatcaac
     4801 cacttacttt tcttttattt ttagtagaaa tctacagtgt gtttttgtga cccgtcactg
     4861 taaataataa catccaccat aaaacctttg ttgtaccact gcgaatttgg atcgctaacc
     4921 tctgcattag cccaatcata ggcttcatct acagtgttaa atacctttcc actattacca
     4981 atctctccaa ctacaattgt tggctcttca ggtgtcgttg tgccaccatt tccgccttga
     5041 tcgcctccac caggattgct tggttcttca ggcgttgttg ttcctccacc tgtgcttggg
     5101 ttagaaggag ttgtttctcc tccagtattt ccaccattac ctggttgtgt aggtgtttga
     5161 gtagtgccac cattgttttc tggtgtactt ggcgttgttg taccaccagt tgttcctgtt
     5221 tgatctccag ctggtgttgt tggtgtcgtt gtatttggtg tcgtttgtgt atttgaagtc
     5281 gttccttgtc ctactggttg attcggatca actttgtctt tctcttgaat cactgtttga
     5341 gccacagttt cacctgcgct gttttgtaca cttgctacat tgccattaaa tactaattta
     5401 tttcctgcat agattaaatc cacatttgta atcttattca tgtccgctaa tttttggaca
     5461 gtaatatttg ttgcttgact gattgcgctt agggtatctc cccagacaat tgtgtattca
     5521 ttccctttaa tttctgcttt gatttgttcc gccgaacgag gtgtccaaac tttgttttct
     5581 gctgcttcag cattcgttcc cacagcaaga ctaactcctc cgactacgat tcctagtgcc
     5641 aatactttca acattttcat tgtttttcgc ctccgatttg aataaattta tttacaaaaa
     5701 catagtacca aacttatgtc tatcagtcga tatatttttt gtagattttt ttattttttg
     5761 atatactaac tttggtacta aaataactaa aaggaatgtc acacatgcaa cttaataatt
     5821 taaatgaaat acatgattgg gctcaaaata atttacttaa tcgtaaacaa gctgccaaaa
     5881 tcactggaca aaactatgat gctttttcta gctctttacg tagaaataat atcaaaccat
     5941 ttctagaaat aggcgacgaa cctggtcctt ccatcattcg cttatatcta agagaagata
     6001 ttgaaaaata cgcaaaacag ctcgaagaaa aaagacagaa acgtcaatga ggcgttttct
     6061 gtcttggatt tacaagtaaa acaataataa acacttttac aacggaggtt ctatgaataa
     6121 gaaatacatt gtttcagttg gttcaatcgt actgattgta ctcgctatta ttgctttttc
     6181 tgtatccaac aaaacagaaa cacagccaca aaatacggtt caatcgacta ctacaagtca
     6241 atctagcgat cattccgtct atccttcact aaatagttta aaagaaacgc atggacaaga
     6301 tttaattgat cttggttctt ctcaaattac tactgtctta tttgaaaaga aaaatattgc
     6361 gtttattact cctaaagaag gaactgacct tgaattttct atcaaagtaa caaataccaa
     6421 agaaaattta ttctccattc ctgacattca atatacccct aacaagttaa agattgggga
     6481 tacgtttggt ttggctaaag taaatgaaca atactttgct tactcactga aataagtttc
     6541 taatagcaag aggaaaaacc ctcttgttat ttttatcaca taatcatttt ataaaaacgc
     6601 ttcgaaaata tcaacatttt tgaagaaact atttttctaa tttctcaatt tcttccaagg
     6661 gattttcgct aatgacccat acaggcacag attgctctcg aggctcccct tcttctaacc
     6721 accaacctgc ataatgaact tcttcctcct cagcattacc atgataccaa ccaacaaagg
     6781 attcatctac aacgaatgaa atttcttctt ctggatcaaa taattcattt ttccatgctt
     6841 caattgcttg ttctttggtg tacttttgtt tgttaaatac accacaaatt tcgtcatcta
     6901 cataaaaaat ttcaaaatca aaattactcg ttccttttac tggcatctta acttttcctc
     6961 ctttgactca cttcaataga tataccactc atgttcattc cattgtttac tagttaattg
     7021 gagttttatc ttagggcttt gtgcactctt tccaaaaact tgagcgtcgt ttttttcttc
     7081 taaccaaaaa aggtaatttc ttacattcgg tactaaatgt ttttcctgca tttcttcatg
     7141 aaaaacatat aattctgctt catttttcaa ccaattcatt attcattccc tcattcactt
     7201 cttcataccg ccattcagat agtgagattt ctttttgttt ctccacttgt cctataaaaa
     7261 tcgctgtatt caacaagctg atcacactta ataataaagc aatgaaacta atggttacac
     7321 ttactctttc acattttgtc attttgtttc cttctttact cgtttgttat ttcttgtaat
     7381 aggtctaata actccttcac cgaaatttgc aaatggttcg caaaaatttc aataaaagca
     7441 ctcgcacaca ttaccagacc ttcaactgat atatcactgc gacaataaaa ttctggagaa
     7501 acactgattt ctgattcttt cttacaattg aacgaaaaaa ctatttttga atcaaattcc
     7561 ggtaattctt ccacaactat gttttgggat tctaccaaga aagggagtgc ctgttctaat
     7621 gcttcttctg gtgtttgagc agatagaata attgcttctt gttcttttat ttcattgttc
     7681 tcaaatactt tataactttt tttcattgtt agttcctctt tcattcctct ctcaattagc
     7741 taagccgtta ctggtacgcc attctctaaa tttagtatcg ctaatcctta atgctcgtcg
     7801 aatatcaatc attcgataac ctattgcacg aagttcttta tatcttttag cagttagcgt
     7861 ctctaacgtc atttcaggca atgggttcga atcaaaaata gaattcgaac cgattatttg
     7921 ggccaattgt tgtacttgta acagctcttc tgaacttaat tcagtgattg ctcgattgcc
     7981 tatcattgca tacattttct gtcgaatata cttcttttct tgtttagaca acatttctat
     8041 ccactcctat tttcattatc atttgttacc tccacttcag taaatccaca aataaatgaa
     8101 ttcatattcc aagtaccatt cccttcaact acaacttttc tcctgtcctc tttcggatat
     8161 tcaagaatca gaccgtttac caatatgttt ttcactacta ctaatttgtt tgtataccgt
     8221 ggaattgctg ttccgatata cttcacttta tttcctacgc taattgtcat ttcctcactc
     8281 ctattctttt ttatccaaaa ctttttcctg tcctattcct gccaacgaat atacttcact
     8341 gcatgcttaa acaaaacaaa atgttgccct gttttatttc cgtcttttac ttgttccaca
     8401 tacagttcat atttggtcac accttttaaa acgccagata ctttttttca tccattgtca
     8461 caattagttt tttcatcaat cgattgatac tgcactgctc atttttcttg ttcatatagt
     8521 attcctttct ttttcaaaac tagcctcttt tgtttactag ttttcatcaa tcatcaacga
     8581 tttaacagat tttttctttc gcagggtttc tagcgtcagc aaccccaaac tctaaaaaag
     8641 tgcctttgct cccttgaaaa acgagcggac gattgtactc gtcatcatat gtttttcata
     8701 ctacctgatc cacgcaaata accttttttt gttctgtgcc acacccatcc atacgtatcg
     8761 cttgagattc accatacttt tttaggtggt ttatccttcc taagtccctg ttagacacgc
     8821 tgattaactt agaaagtcct gtttgtatcc aattttcaaa gatctcccaa aagaaataag
     8881 taggtttacg tgtatcgctt ctgttcaaag tacctgtttg tacttgagta tttctactcc
     8941 cctcgttcaa gggattcctt ccaccagcta gcttttcctt tcatcatcaa atgttcctga
     9001 aaagcattta cgtttcgtgt ccgtgtgctt acttattctt ttttcgatag cgattatccg
     9061 ctcgctactc gctattttat actttgtttg ttagctcaaa taaaaactaa caaacaaaaa
     9121 aggctagttt ctttccgttt tattttttta actgtcgata aatagttgtt agtttaattc
     9181 caagttctaa aaccaatacg cccaacaaaa ccacaggaaa aacaattggg gcaaacaagt
     9241 aaaatcggat agttttcatg tcttccctct ttctttttgg tgtcgctgtg gtcgttcttg
     9301 ccgttcaata cttcgataca ttttttattc attatttgct cgttttgttt tcctctggac
     9361 tgcttttctt gctttttgaa tactttatac gcttttcgtg cctgaattaa taggtcttgt
     9421 cggcgtttca ttcgtgagaa aacaatattt gaaaccatca aatcctggtt cgaacaacca
     9481 cgattcgcaa taattttccc atcaaaaaaa atagttttca cttgagaaaa aaacagttgg
     9541 aaatcttgtt ttgttgctaa tttttttgtc aaaatcactg tgcttttccc tgttgaatct
     9601 acaatgattc ttcgaacaat ccgattcgat aacttccaac gttgccgatt aaacaatgtt
     9661 acttcacaac aaatatgacg taagtgatta ggggtcattt tcatgtgtcc aattcctcca
     9721 accaattatg catcgaaatt ttgggcaatt gtgacagccc ataaatctgc gtttccaggt
     9781 cttcataact acaagaacgc ttggcttgaa gtaatgaata ttctgcttga tctgatagtt
     9841 ttgttgccat tggatctaga ttcaattctc ttacacaaca ttcccaaaaa ttcaatagcg
     9901 ctctaaaata aaatcctttt cgattctgaa ttgcttttcc ttcatgacga gcaatttttt
     9961 cattttgaat acaccgttga tacgttttgc ttaaatcttc tgcaaatgtt tcccctaaaa
    10021 ttcgatactc gcttccatca gataaaccat gtgtttgatt atactttcgt tgccgctttt
    10081 ctataattcg tttactgtcg tatatcatgt ttagctgctc tttcgcttca cgccattcag
    10141 ctgaactgcg aacaatccat gtcaaaacat ctttcggaac aaacgtactt tcgctgtaat
    10201 gagcaataaa ctctttttgc caatcatcag ccatttcaac aggactagtc gtatcgattt
    10261 ttgtatctag attttctgta tctagattgt tagaagaatt attaaataat tgtgcagaat
    10321 tttcacatgg atttttttcg tcaaaatcat cgaaaatcgc taaattatca acagattcct
    10381 cctcttcttt tccttgtgca gaatctacac tggttgagtc aacaggacct aatgaataca
    10441 catcattttc tgtcgtatct aattctccta gatacaaccg agctttgttt ctttttcctg
    10501 tttgttgatc aaatcccata tcctctattt ctagtagctc atattctttc aattcttttt
    10561 tgattgtatt aacagaacgt ctgcaacaat tcaatgcatt ggctaattct tgttccgtaa
    10621 attcaaaata aacaaaacct tgactatcta cttgatttcg ttgaatggct aaatctaagc
    10681 gtgaccgaaa aaccatatac gcgatttttg ctttatcaga caatgagcga tatttttcac
    10741 ttgtaataaa tacctttgga aattgatagt ataattcatt gtatttacga gttgcggtaa
    10801 atctttttgt tgccatcgtt attacacctc atcttgtcca tatattaaga aagaattttg
    10861 gtgttggtag acacgaacat cttccattgt agcaatcaat tcttttagat aaattcgacc
    10921 gaattcaatt gttagtaagt cgtaatcttc taattcattt ttgatttctt caatttcttc
    10981 ttcagacaca ttgagtaaaa tggctaattc ttgattggta taagaaatat agacatgttt
    11041 cttttcatca atccgttttt caattaagga ttcttctaag tactcattca gtaagctata
    11101 ggcgtatttt gcttgtgcag tcatcgcata atattttgac gattttataa agaatagttt
    11161 tggcatatga aaagttgctt gtgtcagcac atctgttgcg cgaaatttgt tttttaccat
    11221 attttttcct cctagaaaaa cacgcaaaaa aagctctgta taaatcgaat tacacagagt
    11281 ttcttcttgt tgatatttaa tttctaaaat acgtttcggg taaaaactaa acacttacta
    11341 atatttagat tgtagaatct tcgccttcat ttattcattt taaagagaaa aagatcaagt
    11401 gaaatgtgcc caaaagtttg attctattaa tttttaaaca ccattcataa tatatttcct
    11461 ataatgtaaa aagcgtcgat acttattaaa gcatcgacgt tatttttata gtaaatttat
    11521 caaaattttt gccaacattt tttccgattt ttttccctat ttctatcttt ccttatcttt
    11581 cttagttaat attatttgat gttaatatat cactactaat attaaaaaac attgatttat
    11641 cataggata
//
LOCUS       DACDSR010000082        11643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDSR010000082 DACDSR010000000
VERSION     DACDSR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.082"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            316..675
                     /locus_tag="IWK15_002193"
                     /pseudo
     CDS             316..675
                     /locus_tag="IWK15_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="IWK15_002194"
     CDS             complement(970..2103)
                     /locus_tag="IWK15_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9547758.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="IWK15_002195"
     CDS             complement(2094..2762)
                     /locus_tag="IWK15_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547759.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWK15_002196"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWK15_002196"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9547760.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWK15_002197"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWK15_002197"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9547761.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWK15_002198"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWK15_002198"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9547762.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWK15_002199"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWK15_002199"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9547763.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="IWK15_002200"
     CDS             complement(6138..6911)
                     /locus_tag="IWK15_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9547764.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="IWK15_002201"
     CDS             7076..7555
                     /locus_tag="IWK15_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9547765.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="IWK15_002202"
     CDS             7612..7914
                     /locus_tag="IWK15_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9547766.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="IWK15_002203"
     CDS             7936..9393
                     /locus_tag="IWK15_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP9547767.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="IWK15_002204"
     CDS             complement(9986..10738)
                     /locus_tag="IWK15_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9547768.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="IWK15_002205"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="IWK15_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..>11643)
                     /locus_tag="IWK15_002206"
     CDS             complement(11199..>11643)
                     /locus_tag="IWK15_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9547769.1"
                     /translation="HKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHL
                     FKSMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDF
                     RDMDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         3343 a         2510 c         1994 g         3796 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc atttagccct
    11401 ctttgcgaag gtgttcctgg atctgcgaaa aagatggcaa tatcgttcga gttacttata
    11461 gatcgccagt tggaaaattc ttttccacaa tcaaatgtca tggatttaaa caagtgttta
    11521 ggcatggatt tgaaccaatc atttaacctg ttttcgatat cttcggctcg tctgcctgtc
    11581 ggtttcaaag taataataac cttggataaa cactcaacca atgtaataac agcgctcttg
    11641 tgt
//
LOCUS       DACDSR010000083        11469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDSR010000083 DACDSR010000000
VERSION     DACDSR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.083"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            422..691
                     /gene="rpsN"
                     /locus_tag="IWK15_002207"
     CDS             422..691
                     /gene="rpsN"
                     /locus_tag="IWK15_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9547770.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            706..885
                     /gene="rpmF"
                     /locus_tag="IWK15_002208"
     CDS             706..885
                     /gene="rpmF"
                     /locus_tag="IWK15_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9547771.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            917..1294
                     /locus_tag="IWK15_002209"
     CDS             917..1294
                     /locus_tag="IWK15_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547772.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1315..1464
                     /gene="rpmG"
                     /locus_tag="IWK15_002210"
     CDS             1315..1464
                     /gene="rpmG"
                     /locus_tag="IWK15_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9547773.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1488..2471
                     /locus_tag="IWK15_002211"
     CDS             1488..2471
                     /locus_tag="IWK15_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9547774.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2501..2623
                     /locus_tag="IWK15_002212"
     CDS             2501..2623
                     /locus_tag="IWK15_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9547775.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2641..4164
                     /locus_tag="IWK15_002213"
     CDS             2641..4164
                     /locus_tag="IWK15_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9547776.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4188..4427
                     /locus_tag="IWK15_002214"
     CDS             4188..4427
                     /locus_tag="IWK15_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547777.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4620..5081)
                     /locus_tag="IWK15_002215"
     CDS             complement(4620..5081)
                     /locus_tag="IWK15_002215"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9547778.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5276..5494)
                     /locus_tag="IWK15_002216"
     CDS             complement(5276..5494)
                     /locus_tag="IWK15_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547779.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5612..7228
                     /locus_tag="IWK15_002217"
     CDS             5612..7228
                     /locus_tag="IWK15_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9547780.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7469..8809)
                     /locus_tag="IWK15_002218"
                     /pseudo
     CDS             complement(7469..8809)
                     /locus_tag="IWK15_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9085..9240)
                     /locus_tag="IWK15_002219"
     CDS             complement(9085..9240)
                     /locus_tag="IWK15_002219"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9547781.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9536..9868)
                     /locus_tag="IWK15_002220"
     CDS             complement(9536..9868)
                     /locus_tag="IWK15_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9547782.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10382..10573)
                     /locus_tag="IWK15_002221"
     CDS             complement(10382..10573)
                     /locus_tag="IWK15_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9547783.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10667..11053)
                     /locus_tag="IWK15_002222"
     CDS             complement(10667..11053)
                     /locus_tag="IWK15_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9547784.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3816 a         1803 c         2197 g         3653 t
ORIGIN      
        1 aagaatcagt atgattgaat tagttcactt aatgtctatt cttataatat aaggaataat
       61 caaagtttta aaaaattggc gataaaccag ttaaacataa aagattgaat aaaaagttat
      121 agtttctttc atattgccgt tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt
      181 tgattggact aataaaagtg aattcttatt tttcagagcc taaactgcat attcttttaa
      241 atgatttgat tatctctatg taccgataaa ttcgttttca aatagttatc tattcatatg
      301 agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa agtatcagtg
      361 tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta
      421 tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta
      481 tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg ctaaattgcc
      541 aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc gtccaagagg
      601 gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc atcaagggtt
      661 gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc agtaccagca
      721 agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt agtaaaacca
      781 gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt atctttaaaa
      841 gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa tatataactt
      901 ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag gattaaagta
      961 ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt cgatggtaga
     1021 gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa atggattagt
     1081 caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa tctatccaat
     1141 ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg agatacctgt
     1201 tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt ttgaagatgc
     1261 acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag atttatgcgt
     1321 caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat
     1381 aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg
     1441 gcaattttta aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg ggcataccag
     1501 ttacaatcgt ttctgggttt ttaggggctg gaaaaacaac gctaatcaat caagccttgc
     1561 aagtctcacc ctaccccagg gaagaaatta ttataattga aaatgagttt ggtgaagttg
     1621 ggattgatca caaattatta gttcatagcg aggaacgggt gttacaacta aataatggat
     1681 gtatgtgttg cagtcttcgc agtgatttat tagcaatatt gatttcttta ctggaaacaa
     1741 tagaagaaca tcaacaacca atttcacaaa tcatcattga aactacaggt atagcagatc
     1801 cccaaccaat cattcaaaca cttttaacag cgcctcaact aagaggaaga gtatatatcg
     1861 atagtttatt gactgttatt gatagtgaca gttttgaact tattgaaaga gaaccacaag
     1921 cccttaagca acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt
     1981 tatctgcact aaaaaaatta cagagagaaa tcaaaagtat taacccttta tccgatattc
     2041 gaatgttcac acatagtgaa acgattcaaa aaaaggattt ttataatttg aaaaaattta
     2101 atcatccctt agtttttgaa agtgattcat cagaaaatca ctataatggc aaatactatc
     2161 atcaacacca tcaagtacat gagtttaaat caattctctt aacttcagat caagacataa
     2221 aaaaagattt tttactccaa tggattgact ggctgatttt aaccaaccaa gggagcctgt
     2281 atcgttttaa aggattgatt gctttacaag agcaggattt tatagttgcc atgcaagagg
     2341 taaacgaaca ggttaatttt caatacacca ctcgacaaaa agaccaaacc actattattc
     2401 taatcggaaa aatgttggat gaagaaagga tcaaacagag ttttgataaa ttactggcgg
     2461 aaagcaaata atgataagta gtgattgggg gtaatttcta atggagaaaa cggatatatc
     2521 tagtgcatat cgacgactta aaagcccgaa tattaaaact agaaaaaggg cattaaaaat
     2581 tatcaaagaa cataaacgaa ataaaatgaa gaaattagca taattaagga aggaaaaaga
     2641 atgaagaaaa tcagtgtagg acttattggg atagcagctc taggattatt gggagcttgc
     2701 tccagcacta atgatgcaaa agtatcgaat gataaagatg gaaaattaga aattgtaaca
     2761 acattctatc caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat
     2821 ttaatggttc cagcgggatc agaacctcat gactatgagc cctcagctaa ggatatggca
     2881 aaagctcatg atgctgatgt ttttgtatat cataacgaaa acatggaatc atgggtacca
     2941 aaagctaaag aaagttggaa gaaagctggg cctaatgtag tagaaggaac aaaagacatg
     3001 attttattgc caggtagtga agaagaagat catgatcacg gagaagaaga tcaccatcat
     3061 gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc
     3121 aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa
     3181 aagtatttga ctaaactgaa acggttggat gctgactata ccactagtct taaagaagcg
     3241 aaacaaaaaa gttttgtgac gcaacacgca gcctttggat atttagcttt ggattatgga
     3301 ttgaaacaag ttcctattgc tggactaagt cctgaagaag agccatcttc tggtcgatta
     3361 gctgaattaa aagagtatgt taaaaaaaat aagatcaatt atatttactt tgaaaagaat
     3421 gctaatgata aaattgcaaa aactttggcc aacgaagctg ggataaaatt ggaagtactg
     3481 aatcctcttg aaagtcttac aaaagaacaa atggataatg gtgaagatta tgtttcagta
     3541 atggaagaca atctaaaagc gttagaaaaa acaactatgg ttgctggtga agaggtagtt
     3601 ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg ggtactttaa agatgccgat
     3661 gtgaaggatc gcgaattgag tgattataca ggcgaatggc aatctgttta tccgttgtta
     3721 aaagatggaa ttttggatga agttttcgat tacaaagcaa aaatcaataa agacatgaca
     3781 gctgccgagt acaaagatta ttatactact ggctataaga cagatattga tactatcaac
     3841 attaaagaca acaccattga ttttgtagtc aacggtgaac atcatcaata cacatacaaa
     3901 tatgttggat acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt
     3961 gaaactgatg atgcaggtgc tggtcgtttt aaatatattc aatttagtga tcatggaatt
     4021 gcaccaagta aggcagagca tttccatatt ttctttggtg gagaaagtca agaaaaactc
     4081 tataatgaat tgcataattg gcctactttc tatccagctt cattgtctaa acatgaaatt
     4141 gctcaagaaa tgatggctca ttaaaattca atttttggag gaaatacatg gtaagaaaag
     4201 tattattgtc tatggattcc aagaatattg atgcttatcg gcgttatcga tttgcaaagc
     4261 aaatgagatt aaatgggagt acattagctg atattgctgc aactttacat gttagtattg
     4321 acacagttca tagagtatta aaacgggatc ctgattgttg ttttatcaag gtcaatactg
     4381 gaagaagtag acgtttagca aaacggcata atagcttggc caattaattg ataataccag
     4441 agtagatcat ttttccctta tttggatttg atctgctttt tattatgtct tgaattgtca
     4501 aatcaagcgc aacactttat caaagaagac ttcataaggt gttctccaat ctaaacactt
     4561 tctaggtctg ttattcagtt tttcaatcat ctgatttatc gttgagttat ctaccgtagc
     4621 tatctctttc gattttggca agtattctcg aatgagtcta ttcgtatttt aatttgttca
     4681 ttgttgccaa gggcatgagg atctggaaaa taaaagggca tattattcaa tgcttctgta
     4741 actgcgaaat gattggagaa ttcttttcct ctatctggag tgatcgtttt gaccgcctca
     4801 tttggaatac tgcttaaaag ttcaatcatt ttctcgctca cataagcggc tttctttctg
     4861 gggaccttct ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt cactaaacaa
     4921 gtaccaccag ttttgccagc tacaatatcg gctttccaat gaccaaaatc acttctatca
     4981 tctgcggatt ttggacggcc atggatcgaa tgagtaaggc aaatctttcc acgagactcg
     5041 acatgatttt ttgtatgtct tgtttttcct cgatggtgca atgaacggat caggccttta
     5101 ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg
     5161 ataaaactaa ttgaaaagtc catatgttca tgcttcaaac gattcgagat ttcctctggc
     5221 gaccactgaa aatcgacaaa taaatgacga acaattgacc aaactcacgg atcatttaga
     5281 atgtgataac gtccgcattt ttttcttcga taagcataat ttttttccgc tttggaaggt
     5341 gaatagtttt tttcttgttt cttctcagtt catgtaaaat agtaaacggg ctatgctgaa
     5401 tgtcgttgcc aattatacta atagaaaatc cttcttcgtg aagcagaaag attctttctc
     5461 gttcgagtat agtaagatga cgatactggc tcataattat tttccttcta ggagtgttct
     5521 cgacaaactt atactagttg gatatcgatt atgactcatt tttatgtgtt gcacttaaag
     5581 tgtaaattca aggtatagaa agaggttccc tatgaaaaaa atacctatta caatcttaac
     5641 tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg
     5701 aaaggaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt
     5761 cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat
     5821 ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga
     5881 cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc
     5941 aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc
     6001 cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat
     6061 tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt
     6121 aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac
     6181 caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat
     6241 taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat
     6301 agatgagact tcctattcag tgggctggtt caaagagtta gagcttggct atcaaaatca
     6361 tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct
     6421 tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc
     6481 aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg
     6541 tagctataaa agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg
     6601 tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt tgtttggtga
     6661 acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca
     6721 acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt
     6781 tccatggaaa aagtctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc
     6841 tggtgtcaag ataggcgatg tcataattat tggcattttg attattacct cttttattcc
     6901 cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc
     6961 catcgttcga attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa
     7021 actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag
     7081 aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc
     7141 aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa
     7201 agaaagcaca gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga
     7261 aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac
     7321 aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag
     7381 ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt
     7441 caatagaata atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg
     7501 ttctggcttt agtcagttgg gtagtattct tagttttctt aaaatatttt cctcgatata
     7561 aaaagattcg ttctcggtag ttttgaaagt ttctatagcc ataggcaatt cgttttagta
     7621 ctttgatatg gttattcaaa cattctatag ggccgttgga atagggaacg cgcaacgctc
     7681 gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac tggttggtag tgctctggca
     7741 actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgataa
     7801 cggaaagaaa atcctggtag acttcgtagc ctcgctttaa ggaatcatca taagataata
     7861 gccgatcgac tatctctgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat
     7921 gctgaccttt caattcccac gcattctttt gaagtagttt ccagtatttc tttaagtgct
     7981 tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaaag
     8041 cccgaccaat gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac
     8101 actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga cgcacttgtt
     8161 cacggacttt tatagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt
     8221 ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg
     8281 agacattttt cactgatttg aattcatcga aacacaccac ttctggtaac agctggctca
     8341 aagaaatggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt
     8401 gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct taatagctca agaatggctt
     8461 gtttgactct gcgagcaatg gagcagcgac gatcactaac tgaatcttct gctaaaaaag
     8521 tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt
     8581 tactgacatc attcaataag atggatactt ttttccagcc ctatttaatg atcgtttgtt
     8641 cattttgata gtggcgatga aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa
     8701 caaaacatgt ttcgtcattg attcgctctt tgcgaaaggc atcttcatga aagataagat
     8761 ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact
     8821 cctttgaatg gttttcgtcg actttattct aaaggatttt aaggtgtctg tttgcgttta
     8881 ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc
     8941 ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt
     9001 gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt
     9061 atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc
     9121 attgtcaaca ggagtcccag tttggctcac tgaatgtgtg atgtggtggt cgttcaacag
     9181 ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa
     9241 ttctccttaa taaatttgta atggacggac gacaatctct tttttcatgt gtgaatctac
     9301 catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc
     9361 gtcattacat ggaatgtaag taatatccat cacccaaaca gcatcaattt ggttctgttc
     9421 aaattatcga ttcaacattt ttttaccatt tctttgattg ttttttctct accatgagaa
     9481 gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca
     9541 acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg
     9601 tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt
     9661 caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt
     9721 tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt
     9781 cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt
     9841 taaatttacg atcatatgtt tttctcaata gagttcattc cttccggtaa cgtaactgat
     9901 agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga
     9961 agttgataga taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca
    10021 aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg
    10081 cttttcaatt ctagaggaga agtcctcgtt gaagtaccat agcccagttc tggattcgac
    10141 ctcctgccca cttcatacgt aaataaagta attttaggag tgagactgtg acaaaagtta
    10201 tttttaagct aaaaacgaat taaaaaacgt tgataattaa aatcattcgt tttagattct
    10261 atgaatcatt atagaacgct gtaaaatcga tactttataa ttataaagaa gatagtttca
    10321 aatttattgt tcgaaatttg aaactatctt tttacttatg tcacagactc ccttagttga
    10381 gttattcttt aatttcgaat ttcgagatag ctagatatat tgcaagcata cattcttgat
    10441 aacaatcttg atcatatttt ccagttctcc aggattgctt atgaatcaaa gagctaaatc
    10501 ttttgagaat tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg aaatcttctt
    10561 ttaaagaatt cataaatcta ccttcttccc atggacttat aatctatttc tttgggaagg
    10621 aggagaacga gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt
    10681 tttgaatttt tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaatcccca
    10741 gaacctttcc aatttcttta tcagttttat caaagataaa tttttcgata agaaaaattt
    10801 gttcaggttc tttcaacaaa ggaaggattt ttgctaattt tatatcttca acaaggagag
    10861 tcacatttaa aacattaatc ggttgtttaa attttatagt atctttagta aacaaatatg
    10921 gtgggagttg atcaagttgt tcatgttttt gaatttcttc ttgtttttta taataagtat
    10981 tagcggtgtt caaaatgacc ttttcaaaga atcgaatcaa ctgggcattt acgcgttcgt
    11041 cgttatctga catattgcac acctcctcca ttaagattta aagggaaaag aggcaaaaaa
    11101 cacgaaaaga agctaatgca attatgctgc gctgcgaata acttctataa gctcacatgc
    11161 attgatagga ctgctgatgt ttattttcaa aaatgttttt ttagttgata atggatagtt
    11221 atctttatac ttagaaaaga aaattcatat atcttcttaa atatcaaaca tgtccttatg
    11281 gctaagaaaa tgtcttgagc tttttacgta taataaatct tttctaaaac ctccttttgg
    11341 ataatattct tattcgtgta tagattgtat tttttatttc ttcccccttc ataattaaca
    11401 actagaaaag agttcgaata gacacgtatt tgaacgaaaa tttttattgg attcccaaat
    11461 tggaatatt
//
LOCUS       DACDSR010000084        11220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDSR010000084 DACDSR010000000
VERSION     DACDSR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.084"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            732..1205
                     /locus_tag="IWK15_002223"
     CDS             732..1205
                     /locus_tag="IWK15_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9547785.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1214..1441
                     /locus_tag="IWK15_002224"
     CDS             1214..1441
                     /locus_tag="IWK15_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9547786.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2077..2448
                     /locus_tag="IWK15_002225"
     CDS             2077..2448
                     /locus_tag="IWK15_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547787.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2704..2946)
                     /locus_tag="IWK15_002226"
     CDS             complement(2704..2946)
                     /locus_tag="IWK15_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9547788.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2978..3880)
                     /locus_tag="IWK15_002227"
     CDS             complement(2978..3880)
                     /locus_tag="IWK15_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9547789.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3893..4081)
                     /locus_tag="IWK15_002228"
     CDS             complement(3893..4081)
                     /locus_tag="IWK15_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9547790.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4095..4658)
                     /gene="amaP"
                     /locus_tag="IWK15_002229"
     CDS             complement(4095..4658)
                     /gene="amaP"
                     /locus_tag="IWK15_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9547791.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4696..5589)
                     /locus_tag="IWK15_002230"
     CDS             complement(4696..5589)
                     /locus_tag="IWK15_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9547792.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5667..6605)
                     /locus_tag="IWK15_002231"
     CDS             complement(5667..6605)
                     /locus_tag="IWK15_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9547793.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6639..6989)
                     /locus_tag="IWK15_002232"
     CDS             complement(6639..6989)
                     /locus_tag="IWK15_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9547794.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7022..7924)
                     /locus_tag="IWK15_002233"
     CDS             complement(7022..7924)
                     /locus_tag="IWK15_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9547795.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7917..8774)
                     /locus_tag="IWK15_002234"
     CDS             complement(7917..8774)
                     /locus_tag="IWK15_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9547796.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9108..9917
                     /locus_tag="IWK15_002235"
     CDS             9108..9917
                     /locus_tag="IWK15_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9547797.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9957..10454
                     /locus_tag="IWK15_002236"
     CDS             9957..10454
                     /locus_tag="IWK15_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9547798.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10839..10912)
                     /locus_tag="IWK15_002237"
     tRNA            complement(10839..10912)
                     /locus_tag="IWK15_002237"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10876..10878),aa:Ile,seq:cat)
BASE COUNT         3277 a         2100 c         1878 g         3965 t
ORIGIN      
        1 tcagtaaaga attcagttgg actactacat tatgggaatc tctattcttt ttcatcttcg
       61 tatttttgtt acaaaattat cgaaaaaaat aaacatgtgc catctatctg ttggcacatg
      121 ttttaggatt tatggtactt tttattcggc aatccatttt atttttctac ttcttcttaa
      181 tacctctaac acttttgatt tataaactaa tccaatgcat ctacttgcgc ttattaagct
      241 atttcagcta gatagtacca ccccatgcgt caatgcttga ttttcctttt gtataacatt
      301 gtataagcaa taaaggacaa ataagtggtg attacaccaa accattgaat aagaaaaatt
      361 ctgatacgta aataaacatg aacttgaaaa aattaatgaa tcggttagct actacgtagt
      421 tgtctttttt ctcatacagt tctgttaact tacacttctc ttcgctagat aataaaaaat
      481 cctccaactg tattgtccaa tataatccca ggcatctaaa ctagcattat agaagagaca
      541 cacatagaat ctaaaataac tggttttaaa tgtttcttac aatcaaaaaa agctgttaaa
      601 tgcactccac accttcatac tctatcattt tttataaacg atatattttt attgtttttc
      661 gataaaaaat agttgtttta caataattat tctgctatat tttagctatc aaagataaaa
      721 gagggataaa aatgaaattt aaaacattgc tattaaaatt tgtcgtttta ggtataagtg
      781 ccgtatttat tttgttcggc gtactttttt atactcagat cgctacaagc gaaaaaggat
      841 tcaattttga ttggcagacc acattatttg tttctgtcat ttactttgcc cttctcctag
      901 ggatcgttat tgcttatttc ttattgaaac tattgaaaaa aatcgaagat aaccaagctt
      961 tttccactca aagcttaaac atactaaaaa aaatcgaacg aaccattttg tttattggtt
     1021 tcgccttttt tggcttactt cctaaattta tgaaagtagc ggatttagac gatgctccag
     1081 gtttgatgtt ggtaggaatc gctgtcgtat tcattccttt tgcagtatac acattaacaa
     1141 gcgtgctgga aaaactgctt gaacatgcga ttttacttaa gacagatcat gatttgacag
     1201 tgtgaggtgc gacatggcaa ttatcgttaa tttagatgta gtattagctc aaaaaaaaat
     1261 gagtgtgact gaattgagtg agaaagtggg cattacgatg gcaaacatct ctattctaaa
     1321 aaatggaaaa gcaaaagcca tccgtttttc cactctcgaa aaaatctgcc aagttttgga
     1381 gtgtcaacca ggtgatttgt tgacctataa aaatgactcg aatactatac cagaagaata
     1441 aaataaagta gaacaaacag taaataaaaa ataatctgaa tgatagatgt gtgagacttt
     1501 tagaagttat ttacgtctat tattcagatt ttttatttcg attgaatacc gttttatcta
     1561 taaataagat atgtactgac tcatacaaag ggggcctttt cttctccatg tactatcact
     1621 tttcctgata caactggttt cctgttacgt tgtagttgga tctgctttta gtataatact
     1681 ttattcattc caccagactg atagcagttt gagcagtcta aataatcatg tagtattaca
     1741 ggccattctg tttcatacct tgaagtgctt aaaacttcag ctaatggccc attgtgttaa
     1801 ctcttactcc attctttttt agtaagctcc catatatact gtaaagcctt ctatatatta
     1861 tgtcatgttg ctttttaaca tttactatgt agttttgata aaatgatttg aataacagtg
     1921 atggacaatt cttttttatt tttaacatta ttttgataat aatgattatt tatttaaaaa
     1981 tacattagat aacatttgtt acgacacgaa aaagcttcac aaacaaatat cgttatttta
     2041 aaatgagggc gaactcacac aattaggagg aatagtatgc catttattgt ttactttttt
     2101 ctttcattac tcgtgatcta tattccatta ccaacatttt tcctgctcgt caatcgtcta
     2161 gcagagcaac atgatacgat tgctgctctt acgaaacttt gtttacttct atttgtttgt
     2221 ctagattact ttacgagcta ttattggcta aaacagacaa gagtaaaaaa gagatattat
     2281 ttcttccttg tctttttcaa tctctttgag tttctgatcc attgcttttt acttcatcag
     2341 tatcatacga tccatttgtg gatcatctgg gttttcaaag tggtcgtatt attttgtatg
     2401 ttcacctttc ctatctcaag aaaatttagg gaggcattgt tttattaaag aagcttctct
     2461 gtatcaataa agacacattt aaggaaaaac ttttaaaaat ctaattggga atttgcccgc
     2521 ataaataaga ggctgtgaca aaagtcttgt cacagcctct tattccgtat aaacagtgtt
     2581 caaaagcttg catcttcgat aatatgccca accaaatgaa aaatatgaga agctattttt
     2641 gtttggctgt tcttattact tgatgaagaa cagctttttc acgacctctt tttaaaagtc
     2701 tttttacagt tttcgagcaa agaatgatac gattagtact aaaatgatgg caccaatcaa
     2761 tgatggcacg attgccattc ctgcaatact tggtccccaa gaacctaata atgcttgacc
     2821 aagtgctgat ccaactaaac ctgctacgac atttgcaata atccccatcg aagaaccttt
     2881 attcgtgatt gctcctgcaa ttgctccgat aattcctccg acgataagtg accaaataaa
     2941 tcccatgttt cgttcctcct aatgttttac ttccttatta ctgaacccgt ggttctgtat
     3001 tttcttttac tttttgtgcg ccgcttccag ctgctgattt tactttatct gtttgattcg
     3061 aagcaaattc accggttttt ttcgctgcgt cagttacttt gtcttgcacc gtttcttgat
     3121 ctttttcaaa ttcttctttt gttttgatgt ccactacgtt agcgttgact tcgatcactt
     3181 ctaaatgtgt catatttttt acttctttgc cgattacttc ttgcatttct tcaaagattt
     3241 tttcgatatc tttgccatat tccacaatga catccatatc aactgctact tgttttttac
     3301 cgacttctgt ttcgatccca gccgttacat tatctgtatt cacaagtttt cctgccacat
     3361 tagagaagaa accaccgttt gcagataata aaccgtctac ttgttcaatt gcgattccga
     3421 tgattttttg cacaacttta tcttcataat tcagctcacc gcgcattgaa tcttcctgtt
     3481 cagaatcatg ctttccttgg ttatttgtat gatcagaacc aagttttcct tgtccgtttg
     3541 tgtggtcaga actaagtttt tcttgtgctt tcgcatggtc agaaggatgt ttttgctctt
     3601 ccacaggttt agaaggatcg attgcatcat gttgttctgg aactttagat tctggagttc
     3661 ctggatgatg aggtgcatgc ttttctgcat catgtttctt ctcttctact ccttgttgaa
     3721 cgttagggac tactggatct ggatgtgcag atactggtgc tgttttgttc ttttcatcgt
     3781 tcacaccatt tgattggttt ggtgtcgttg ggtctggatg tgcagacact ggtttttggt
     3841 tgttcatttt attgttatta tttttgttat tgttattcat ggatgtttcc tcctaagatt
     3901 taaaatagtg atctaagatt ttcgtttgtt tcagataaaa accgatagcg acgccgatcg
     3961 ctgccatgat caagatcaat aaggtcttga aaaagcccac ggtgatccat aaaacggcta
     4021 atatcaatcc aagtacccca aagatgatag ggagcttgta tgtcgaaaat agttctttca
     4081 tttttggtcc ccccctattc tactcgtttg cggtcattgc ttcgtttagc tggttcttca
     4141 aagcctgtgt atttcaccga tactttcact ggctcttttg ttcccaatat gtgttgtact
     4201 tcattttcga tttctttcat caatgttcct gttttaccaa tgagtgctga agttcgtttc
     4261 aactcccctt ttacattgac ggcgatcttg tttttcgttg ctttgacatt tacttttggt
     4321 gaatggatga attcatcttc atgcaagtga gaacgaacca ttccttcaat tgctttttta
     4381 tccaacgtca actccccatc accatgtttc agtagaaagt ttccgattgt ttttggataa
     4441 aacaggaaaa caagtaacgt gataattgtc aaaatagcaa atgcaacggc aagccaaaat
     4501 acatattgtt gcacgtatga attagtgatg gaaaagaatc gaaacttctc tagctgaaaa
     4561 ggcaatggat aggttgcact taatagtatc gcaaacaaaa gcacagatag caaaagtaat
     4621 gttccgatca ctccgatcgt ttttacacct ttattcattt tcgcacctcc tcataggggt
     4681 gaaacgatca acttcttata agttgatcgc ttctccacca gttactccgt aaatttgggc
     4741 tgtaacataa cttgctttat tcgatgctaa gaaaacataa actggtgcta attctcctgg
     4801 ctgacctgca cgttctagta aactttgctg accaaattca gggatcgaat cagaaggttg
     4861 tccttcgtct agttgtaatg gtgtccagat tggaccaggt gctacaccat ttacacggat
     4921 tcctttaggt gcaagctgtt ttgctaaacc gatcgtaaag ttagcgattg aagcttttgt
     4981 tgctgcgtaa tctaataaat gttcgcttgg attgaatgct tgaacagaag ttgttgttac
     5041 gattgctcct cctgctggga tatgcggaac agcttctttc actaaagcaa acatagcgat
     5101 gatgtttatc ataaatgtgt ctttgacttg ctcaagtggt aactcagcaa tagactgaca
     5161 agaaatttgc tgtcctgcgt ttagtaccat cgtatctagc ccgccaagtt cactcacggc
     5221 tttatctatg atttctttag gtgcttgttc gtcacgaaga tcaaaaggta gtaagacaat
     5281 cttacgtcct gcaactttca cgtattttgc tacttcctct gcatcttttt cttctcctgg
     5341 gaagaattga agagcgatat ctgccccttc tctagcaaat gcaatcgctg ctgcacgacc
     5401 gatccctgaa tctccaccag tgattagtac acggcggttt tctagttgat tattgccttc
     5461 ataggattct tctccacaat caggaacagg aatcatattc tgctgtaaag ctggtgtatc
     5521 ctgatcttgt ttaggaaatt tctccgtatg gtacaagttt cgcgggtcga ttaattttgg
     5581 ttctgtcatt gctgtattcc tcctagtttt taaattgtat tgccaaaaca aatttctttg
     5641 tttgatacct gttttaaata gattctttaa tttgtataat ctttccggta taaatggtaa
     5701 cctaaaacta ctgctgctat caaagagcca aagactacga gtataaagcc tagagcagaa
     5761 cttttacccg gtaatcctcc tgtattcatt ccccaaatcc ctgcgaacaa cgccggaacg
     5821 gaaatgatta atgcagctga atctagatat ttcatgatat gatttagatt attgtccatc
     5881 atcccgttga atagatcacc aatactctct aataaatctc gatagatctc gatcattttt
     5941 tctgtttgtc gctgtctcaa atggacatca tagagtagct gttcatcgtc taaacgtttg
     6001 atgaaagttt cagaattcca taattgatcc attgtttccc gttgatctct taaggtatga
     6061 tcgatatata cgatgtctct ttcaagatct gcctgtttat acagttcttc attttccgtt
     6121 gttttacgag ctgctttatc caaagcgtca attcgctttt tcatctctct tagttctttc
     6181 acgtaatggg tgtaaatatc taataatgta tacgtgatga tcttttcaaa cgagtttaaa
     6241 tgatttccat gctttgtcag cagccgatcc acaaattcgg tttttctatc gatacaagta
     6301 atcaagagat tatcggaaag tacaaaagat attggcgtta attttttttc gatacgatta
     6361 tctgactgat ctagattaat cactaccaaa gaaaatggat gctctagttt cgtctcataa
     6421 aggcgctcca tccgagaaac ttcttccgga tattcggttc ctacgaaaat atcatcagga
     6481 agctcgaatt cttcgattgc ttgctcaatc tcttcgggct ttgcactatc aagtacgagc
     6541 caattatatt ttttttcatc tgtttctatt acattctttt cttcatccac aaagtagtat
     6601 ctcaatgtat acctcctttt tggactttct cgaaactatt agctgaagaa gcagttcttt
     6661 aatttgatat tataatcgag tactgcgatt tcattttcta atgaaaagct gtcaagctgt
     6721 ttgttgaagt ccatgttcaa tcgtttaccg aaataatttt gctctaattt agatgtatag
     6781 atgtccaata atggatgctc gattttagtc gcatattctc caaattccga aacaaaagga
     6841 atgatttgaa aaaagctgcc ttttgcaaaa gcacctttgt cccttagaaa tggatcggaa
     6901 tcattgataa gcgcgagtgc gatttttgta ttttccccaa ccttttcaat taacgtctct
     6961 actgcatttt tagacattgt aatttccatt cctactcccc ctcttcagtt gttgtcttct
     7021 attatttaac aactacatct gtttttggtt gaacttttgc atattttgga tatttaggga
     7081 atttagctac acaacggaaa gccgtcatcc ctgcatattt ttcgtaatat ttcttcgttg
     7141 gcccttcaaa atccttgctc cacggtttca catgtcctga aaaatggata atgtaaggat
     7201 tgttgcgagt ttcttcgtat tcacgttcac ctgctgctgt tggatgtttt tttgcttttg
     7261 ccatgatata gccttgtgca ttccaacgcg gatgaagtgg taaccaacgg tcatgtaaga
     7321 tagcatttaa tgcatcttgg tcatggaatc gtaatttttc tggattatgt tcgatgaaat
     7381 ctaatacttt ttgcgtaata ttttcatcta accatttttt cacattgatc agcatcaaac
     7441 ctgagttgaa ataacgcatt gatttagctg aaattttcat tttttccagt cgttgatgga
     7501 acccagcgtc ttcaacagct gctacgattg aatcaccgaa gtcaagacgc cataatttag
     7561 agatatcttg taaagcgatc atgtcgcaat ccatatataa gatacgttca acttctgtgc
     7621 cgcggaacaa ttccggaatc gcaatacgat aataagctgt ttctggaata cgatcgctga
     7681 ttaccacatt tgtaaagaat tctttattga ttttcaagaa ttccacatct gagttcattc
     7741 gtgcgttttt cactgagaag cgtaaaagct gtttactttc aaaatcgata tcatcgtcaa
     7801 tcacatagaa ccgaacaaat ttagacgggt tgcaattatc taatactgag acaaataatg
     7861 cagctaaatg tggtacgaat tttgtgttac aacttgcgac tactgctatt tcttttttat
     7921 tcattgatct cgcctacttt cgttagagta ctgtgtgcta atttttttaa gtataaattg
     7981 gtatacggat gatctttcaa cgtattccaa ggtttatcat gtcctgtaaa gtgaacgatc
     8041 gaaggttttt catttccttc tttatactga cgttcatatt tttcatttgg tgctggatga
     8101 cgttcaaaaa tcagcgaagt ctgcatgttc cattttggat gaagttgttc ccaatcttca
     8161 taaagtacag cattcaaagc atcttggtcg tgatagatga ttcgatcgcc attttcactg
     8221 aggtaatgga tagttttttc tgtaatttct tttttattcc attgatcaat gtcaatcacc
     8281 atcactcctg aattgaagta caagtcatcg gaatcgattc ctaatctttc caaagctttc
     8341 acttggcctg gatcgataat agcgccgatt gtttttccat ttaacggctc atcatatagt
     8401 tttacgatat cgtctagtac taacacatct gaatctaagt aaaggacttt cttataatcc
     8461 tctttagcta ataagtttgg taaagaaatt cttaagtaag cggtagttgt gatatggtca
     8521 ctgactagga agtcttcgta gatatctttt tccactgtca ggaattggat acttgcatta
     8581 cttgaatatt tatttaccgt ttcttccaat ccttctttac tatattctga tagaccatcg
     8641 tcaatcacat aaaattttat gcgtcttgtt ttattgcaat tttctaatgc agttgcgatc
     8701 atcacgctca agtaaggagc ataattttcg tctgaagcag ttaccacagg aactacgccg
     8761 tatctcttct ccatcaaaat cactcctttt tctaatcttt ttctaacctg ttctacatct
     8821 agaatattcc tcatttggtt ttaaagcaaa atatatgctt tgttttcatt gaatatttta
     8881 aaattttttt atcacaaaac gcatcggtaa gcgctaacta ttcgtttttc atggcttttt
     8941 attcaaaggt attgaccaaa aactactata tatagccacc tttttcttaa gaatgttttt
     9001 tgaaaaaatt cataatgaca aagcaagtta gtttcatttt tttaaattct catttaatct
     9061 taaggtaaca ggaaaataga tatgtaaaaa gaaagtagga agataaaatg aaactagcag
     9121 cgattgattt agacggtacc ttgcttgatc gaaatggaaa gataccagaa gaaaatatcc
     9181 aagcacttcg gaaatttgat gaaaatggcg gaatcgtaac cgtcgctacc ggaaggaaca
     9241 gcatttctgc taaagatatt ttttccgaat taggaatcgg tggctatttg atttcttcca
     9301 atggttcact gattgctgaa atgaaggatg gaaaaatggc gaaagttctc agacgttcaa
     9361 aaatagatag cgatacactg agaaaagcct tcttctatgc tcaagaagcg aaaatatcca
     9421 tcattgctag ccgagaaacc caagatgatc agctgacatt cgatgaatca aatctagtca
     9481 aagatgaccc ttattatgaa catttcaatt tacagaacca ttcttttgat caaattttaa
     9541 atatgttgga agatccttct cttagctatc ttaaattagc attaacagat aagagtgaag
     9601 aaaaactaca gaagctacaa agagatctgc aagcagatgg tatccaatca gtattctctg
     9661 atccgcattt cttagaaatc acgcctacag gtatcactaa agctcattca cttgtttttc
     9721 tggcggatta ccttggaatc gaatcgagtg acatcatggc ttttggcgat caagaaaacg
     9781 acattgctat gttgaattac agcgggttga gtattgcgat ggggaatgca cagcgacaag
     9841 taaaagaaat tgcaaaccaa gtaacactga cgaatgaaga agctggtgtt gcttatattt
     9901 taaaaaactt ctattaaaaa gaataaaatg acgatatgaa tgtaggagga aagaaaatgt
     9961 tagttattat tgatatgcaa aatcatattc ttgatccaaa cagcgaattt tatattccag
    10021 agtcagatca attggcagaa cgtattgctc aaagattagc taaagcacgc caaaataatg
    10081 aatatattct cttcacacgg gatataccag ttgaactgaa ggacaaagaa gaagaacgag
    10141 aagatttgca attgatccct cttctttctc cacaagaaaa tgaacgagtg attaaaaagt
    10201 attactttac gattccacct gaaactttag ctgaaatcca ggaaacttta tttgaaagca
    10261 aagaagaaca aaaagagatc gaagtagtcg ggattgaaac aaacttgtgt gtactctcta
    10321 atctgatagg cctgcagagt gcttttcctg aagcagactt ttttgttgat ccaatgttag
    10381 tctccagtag aaaacacgga gaatccgctt tagagctgtt gaaagatttc aacgtctcta
    10441 tcgtcgaatc ataaaaatat ctatcttcat ttacgatatt taacacacaa taaaaaaagc
    10501 acgcagaact gttttagtcc tacgtgcttt ttctatcaaa gtagattggc ttttttatga
    10561 cacagagtga aacggcaggc tcacgacctc tttcttaaat ggtgttcaat cagctgaccc
    10621 tcggtattag cttaacaaac ggcaaaatat gaggaactat ttttccgttt gttaagctaa
    10681 tactcaggtc aaaacgctga tttcacaacc tcttccttaa acggtattca aaagcttgca
    10741 tctgcagtaa taagaacagc caagtaaaaa cataagaagc tgtttttact tggctgttct
    10801 tattacttga tgcagggcag ctttttcaca acctcttatg taccttgtag gactcgaacc
    10861 tacgaccgga cggttatgag ccgtctgctc taaccaactg agctaaaggt acatcataaa
    10921 taaaatttat ttttgttact tttcaactat agcacgaatg acaaactctc acaagtttca
    10981 aaatataagt aaaataatat tgttattctt atttctttta cttgtgtcat aactattttg
    11041 ttactttaat ttttatttgc ttattttaga aaaaatgtta ttcttttatt tattctcaat
    11101 gttaggagtg attattttgc catttattgc gattttattc gatttagttg cggcagcagc
    11161 ttattttctc caattaaatt atcaaacctc aggaattctg tttattggaa tgatttttca
//
LOCUS       DACDSR010000085        10931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDSR010000085 DACDSR010000000
VERSION     DACDSR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.085"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWK15_002238"
     CDS             complement(<1..288)
                     /locus_tag="IWK15_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547799.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWK15_002239"
     CDS             430..1548
                     /locus_tag="IWK15_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9547800.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWK15_002240"
     CDS             1676..3052
                     /locus_tag="IWK15_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9547801.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWK15_002241"
     CDS             3453..5033
                     /locus_tag="IWK15_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9547802.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWK15_002242"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWK15_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWK15_002243"
     CDS             complement(5659..5979)
                     /locus_tag="IWK15_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9547803.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWK15_002244"
     CDS             complement(6127..8361)
                     /locus_tag="IWK15_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9547804.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWK15_002245"
     CDS             8840..9106
                     /locus_tag="IWK15_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9547805.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK15_002246"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK15_002246"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9547806.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1912 c         2216 g         3323 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaaa t
//
LOCUS       DACDSR010000086        10890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDSR010000086 DACDSR010000000
VERSION     DACDSR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.086"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            158..1393
                     /locus_tag="IWK15_002247"
     CDS             158..1393
                     /locus_tag="IWK15_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9547807.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2392..2652)
                     /locus_tag="IWK15_002248"
     CDS             complement(2392..2652)
                     /locus_tag="IWK15_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9547808.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2679..3980)
                     /locus_tag="IWK15_002249"
     CDS             complement(2679..3980)
                     /locus_tag="IWK15_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9547809.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4272..4730)
                     /locus_tag="IWK15_002250"
     CDS             complement(4272..4730)
                     /locus_tag="IWK15_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9547810.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4743..6068)
                     /locus_tag="IWK15_002251"
     CDS             complement(4743..6068)
                     /locus_tag="IWK15_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9547811.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6085..7497)
                     /locus_tag="IWK15_002252"
     CDS             complement(6085..7497)
                     /locus_tag="IWK15_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9547812.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7514..8278)
                     /locus_tag="IWK15_002253"
     CDS             complement(7514..8278)
                     /locus_tag="IWK15_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9547813.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8382..9257)
                     /locus_tag="IWK15_002254"
     CDS             complement(8382..9257)
                     /locus_tag="IWK15_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9547814.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9512..9596)
                     /locus_tag="IWK15_002255"
     tRNA            complement(9512..9596)
                     /locus_tag="IWK15_002255"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9560..9562),aa:Leu,seq:caa)
     gene            complement(9614..9685)
                     /locus_tag="IWK15_002256"
     tRNA            complement(9614..9685)
                     /locus_tag="IWK15_002256"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9651..9653),aa:Gln,seq:ttg)
     gene            complement(9695..9767)
                     /locus_tag="IWK15_002257"
     tRNA            complement(9695..9767)
                     /locus_tag="IWK15_002257"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9732..9734),aa:His,seq:gtg)
     gene            complement(9829..9913)
                     /locus_tag="IWK15_002258"
     tRNA            complement(9829..9913)
                     /locus_tag="IWK15_002258"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9877..9879),aa:Leu,seq:caa)
     gene            complement(9934..10004)
                     /locus_tag="IWK15_002259"
     tRNA            complement(9934..10004)
                     /locus_tag="IWK15_002259"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9970..9972),aa:Cys,seq:gca)
     gene            complement(10050..10121)
                     /locus_tag="IWK15_002260"
     tRNA            complement(10050..10121)
                     /locus_tag="IWK15_002260"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10087..10089),aa:Gln,seq:ttg)
     gene            complement(10133..10205)
                     /locus_tag="IWK15_002261"
     tRNA            complement(10133..10205)
                     /locus_tag="IWK15_002261"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10170..10172),aa:His,seq:gtg)
     gene            complement(10208..10281)
                     /locus_tag="IWK15_002262"
     tRNA            complement(10208..10281)
                     /locus_tag="IWK15_002262"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10247..10249),aa:Trp,seq:cca)
     gene            complement(10288..10368)
                     /locus_tag="IWK15_002263"
     tRNA            complement(10288..10368)
                     /locus_tag="IWK15_002263"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10332..10334),aa:Tyr,seq:gta)
     gene            complement(10370..10445)
                     /locus_tag="IWK15_002264"
     tRNA            complement(10370..10445)
                     /locus_tag="IWK15_002264"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10410..10412),aa:Phe,seq:gaa)
     gene            complement(10481..10553)
                     /locus_tag="IWK15_002265"
     tRNA            complement(10481..10553)
                     /locus_tag="IWK15_002265"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10518..10520),aa:Asp,seq:gtc)
     gene            complement(10563..10635)
                     /locus_tag="IWK15_002266"
     tRNA            complement(10563..10635)
                     /locus_tag="IWK15_002266"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10600..10602),aa:Val,seq:tac)
     gene            complement(10651..10722)
                     /locus_tag="IWK15_002267"
     tRNA            complement(10651..10722)
                     /locus_tag="IWK15_002267"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10687..10689),aa:Glu,seq:ttc)
     gene            complement(10737..10826)
                     /locus_tag="IWK15_002268"
     tRNA            complement(10737..10826)
                     /locus_tag="IWK15_002268"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10790..10792),aa:Ser,seq:gga)
BASE COUNT         3476 a         2347 c         1759 g         3308 t
ORIGIN      
        1 ggagtttcaa cttgattaca aaatttaaat ttagaagttg tgaaaacagc gagagacagt
       61 ttaatattca aaatatacat aggctactcg aacgctgtgt tttttagtat actagtaaga
      121 aacgaaggga tagactcatt ccaataagga ggatttcatg aagacaattc tttttgctcc
      181 tgcagtattc aatttagctg agacaacacg aatgattgag gttgccaaag ctatgaaaga
      241 ccgttattac tgtgtattct ttggcttttc taaaactttt gctcctttga tcacagaaag
      301 tggctttgag tatcatttac tagcgccgat ccttacgaaa aaacaagaag aacaaatcat
      361 aaaatttgac cagttgcgta cacttaaaaa cccttttacc aaagaaatgg ttgcctctcg
      421 tatcactagt gaacagagtc tgattgctaa gtttgagcct cgactgattg tgacaggttc
      481 gaatgtgaca atttttttat cggcccgcaa taaaaagatt cctttagtat atgttaaacc
      541 ctatgctttg agtgaacctt tttttcaatc agagcatgcg ctgatgcccc acgagttacg
      601 tttcttcggc cctttaagca atcccctttg gcactggaca aaaaagcgta tagtaaatac
      661 ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa gaaatgcttc ctttaccaaa
      721 aacaagttta caaatgatgg atgctgatct aaatctcatc acaacgccta aactattttt
      781 ggaagaagaa cagctgccgg aaaattatcg aatcgttggt ccaatttttg ctaagctcag
      841 tacttccttg cctgaagaag tgctaacttt tatacagcga aagcaacaag aaagaaaacg
      901 tattatctat ttcgcaatgg gtagttcagg caatccgcgt atgttacgca aaattttgtt
      961 cttcttacaa aaacaaccgg atctagcggt tattgcacct attgactatc tgcttgatga
     1021 cattcaatct gactcagaag atcttttaat aacagaatat ctgtctagtc atcttttgca
     1081 tgaattcatt gatttttctt ttatccacgg cggggaaggg accgtccaaa ctgcctgtga
     1141 atccggcaaa ccgtttatcg gtgtggggat gcaatttgag caaagctgta atattgacta
     1201 ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca gtgactgaaa gaaaactgca
     1261 gattgctctg cgcgaagttt cttcacccaa gattcgtcag caggcacgca cgctgcaagc
     1321 cgtttttcca ataaatggtc cacaacaagc aattcaagaa atcgaaacct tcctgagcaa
     1381 ccacccaata tagtttcaag tagaaaaagc ttttttaata gagactcatc agtaatttcg
     1441 ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa tagtggattg aaaagttaga
     1501 gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc ttaaaataaa agatctgacg
     1561 ctatccaaat gagcataggt agcgtctgcc atttattctt agtcaaattc tttgacacct
     1621 ttctctggtt ggatcatctc aacgtttctt caaaagaaag accataataa ttaggcaaat
     1681 acattttctt aaaatgtgtc atatagatct agaagcatcg ctttgtagag atttgtgccg
     1741 ctagtcccct caacaattac taaatcaaat gtaggattta ccactttatc tttgataatc
     1801 aacctccctc ttctgttgag acaatgatct cgcaatccga tgcatactcc tatataaact
     1861 gccagcaatc cccacatatt gagtagatca tgtcagaatg ttctatgtcg tcagcaacga
     1921 tacactaaaa gtcacgttca ccatgactga ttgctattcc cagcacaatt atttctatac
     1981 acgtttcatg atttgaataa acactcacat catacatatt accattcata caccatactg
     2041 cagcccctac tatatggttg taagctccca aatctatatt tgacttgatc actttggtag
     2101 tagatctact aattctaaat ccattgctga taaaatttcc attttttcct ccttctttct
     2161 gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa gggctgtgac aaaagccttg
     2221 tcacagtccc atattccgaa taaacggtgg tcaaaagctt gcatctgcgg taataagttc
     2281 aaccaaacga aaaataagcc aacatttcac aaaaatttaa gaagcaattt tcgtaaattt
     2341 cttcttattt cttgaagcta accacttctg ttacaaccac ttttagaaca tttaaagctt
     2401 cgatgtgaca aaccatctat gctgctcgat cataaaattg atagcaaaaa ttccacaaag
     2461 tacatatgtc ccccatgaaa acaaaacatt catatgcggt acaatgatct ttggaagaat
     2521 cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg atttgaatat gatatgtagc
     2581 tagcatattc aatataaggt gactagccgt taataaaaag ataaaaacaa aaaataaaat
     2641 cagccaatac attgtttccc accttctaaa aaccaaactt agacagtctg gacttcgttt
     2701 tctccacgac tttgttcatc tgacagcatc tttttatcat aagcttttac aaatggataa
     2761 tagataataa atgctatcaa tgtgttgatc atgatcaaga taacaacacg ccagtcattt
     2821 cctgctgaaa gataaccgcc gattggagct ggtaatgtcc atgttggcaa aacagtaaat
     2881 ctattgacca tttctaattt gactgcaaaa taactgataa tagtcaaaac taaaggaact
     2941 aagttgaacg ggatgaacat gtaaggattc aacatcatag gagctccaaa aattatgggc
     3001 tcattgatat tgaagatacc aggaataata gaaaaacgac caacctcttt caagaatttt
     3061 gatttacaga agaatataaa caaaacagat accacgatcg ttgctcctgc accaccaatt
     3121 gttaccgtcc attgataaaa ctgttcagga gcgatatagg gtaattgggt ggcagctgta
     3181 ccatctgcca atgcctttgc attctcatcc aacatgatca gccacattgg acgaacaaca
     3241 gcccctacaa tactcatgcc atggatacca aatgaccaca acaaatggat caaaaacatt
     3301 ggtagtaaaa caccaagcaa attattccct aaaatattgg ttaaaggctc gaataacgat
     3361 aataaggcag cgtttacatc aaagcccgcg acgcttcgaa tcagccaagt aattgttaaa
     3421 atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa cagcaggtgg cacttgttct
     3481 ggcattttga tagtaatatt tcgattttta aagaaacgat atacttccac tgcaaaaatc
     3541 atagataaga tccccgcaaa catcccagat gctcccaaag aactcatagg taagacccag
     3601 ccaagaggtg cctcttcagg agttaaaacg tttacaggaa cttgtaacat aaagaacgtt
     3661 cctaaagata atataccacc tgtcacacta tctaatccat atgatttagc tagatccgaa
     3721 cccataacaa aagttgcata aatagccatc aatcccatcg tcatcctaaa gggtaaaaca
     3781 atcgttgccg aataaggggc aataaattca ttccatccag gaaacggtaa attgataaag
     3841 accataaaaa atgtcccaat caaaatcaat gacaatgttg aaacaacccc attacgaatt
     3901 gctaataaat gacgctgtgt accaattttt gccattggtg ggacaagttt ctcttcgacc
     3961 catttcatca gcttttccac taaaaccact ccttttcaat aaaatatgaa ccattcattg
     4021 catgacaacg caaagaatgt tcataactaa ttatataatt aagaaatttt atttcaatat
     4081 attctcttaa ataatcaaat ttatttttaa gaatcctgaa aataagtttt ctaaatcata
     4141 ggagcaacaa aaaaattcga taattaacaa aaatcaaaat gattaaatct agtgattgaa
     4201 acatatcatt tcaaaaaaat tttgtaaatt cacccataaa tatcacccta tttaactttt
     4261 ttcatcgtac actaaaattc aaaacaacta tctttaatct ttacttcttg gttttgtttt
     4321 aataaatttc cttcaaaaat gcctggagaa actacaatga tcaccgtatc tttttgtgct
     4381 tgctccaact tcttcaaatc tataatagct aacagtgtcc cttcttttac ctcctggtta
     4441 ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa ctgtatctaa acccatatgg
     4501 atcagtattt ttctgccaga ttcactttcg atcgttatgg catgtcgggt aggaaatata
     4561 ttatcgatta ttcctttaac tggcgcatgg atatatccat catgattcgt tatagcgtat
     4621 ccaactccta tcatttttga agaaaagacc tcgtctttta ctgctgttaa tggaataatt
     4681 tgaccactag ctacagcttt tagaatgttt cttgttttaa agaaatgcac tttttatcac
     4741 ctctacttta atgctggcca atattttttg tttactttga tcatttcatc tagtattttt
     4801 tttgcgactc tggcatttgg tacggtttga ttcaatgtaa atgcttgtaa agctttttga
     4861 taagaacctt caaagtaagc atctactagt aacttctcta ctgcaacttg tgcctccatc
     4921 aatcctttat gaaaatcact gattggtttt cttagtgtaa ttggctccac accagttgca
     4981 ccaacatatg ccggtatttc aacaactgca tctgaccgta gattcggaat cgccccttga
     5041 ttaggaacga ttagcataaa gcgacgacga tcatcattca acaaggaaat agctatatcg
     5101 acgatatatt gtccatgctc cccaaaatta aagttcaata cttcttccac aataccgcgg
     5161 cgtattttat cagccatttc tttggtattt ttctctcgtc catccatgac catattagca
     5221 cgtgtatact ctttgtcagt atgctcaacg accatatcag gatataagta atactctaag
     5281 taattattcg ggatattact tggaaaattt ttcgtgatga ctgacatcat ttgaaaggtt
     5341 ttttgccaag ttttatctcc tatattaaaa tcggcgactt gcatttcttt tgttattagt
     5401 ttttcaataa tctctggcat aatttctcgt tgcaattctt tatcataaat cgatgtatac
     5461 cagccaaagt ggtttaaacc ataatacgta acgatccaat tcttacgatc ataaccgtaa
     5521 ttcactgcaa tcgtttcttc aattgaaata gtcatatcac aggcattaat catttttaca
     5581 ttcggatatt tacgacgaac agcttcagaa acgatcgact ctggatttgt ataattcaaa
     5641 atccatgctt tgggggcata gtcttgtata tgatcgacaa tttccaataa acctttgatt
     5701 gagcgcatcc cataagcaaa tcctccgagt ccacacgttt cttgtcctac taatccatgt
     5761 ttaagtggaa tcttttcgtc cttttctcgc atctccaatc ctccaacacg aatttgtgag
     5821 aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat ccgttgcacg aatgcgaata
     5881 tttgaaaaac cttttctttt cagcataaat ttaacaatca agtacatatc catatttctt
     5941 tcttcatcaa tatcatataa acgtatttca gataatggca accgtttttc attgttcaaa
     6001 atggcttgaa cgattcctgg tgtatacgta ctcccgcctc cagctatagt gataatctgt
     6061 tttttcatca tcatcttttc ctttctattc ttctaataat ttattgaatt ctgccataac
     6121 ataagtcact tctaagccga tgataatttg ataatttttt tcagttggtt taatgatccc
     6181 gcttatccct aattgcttga ttcgctgttc ctcgaccatg gtgggatcca ctacctctac
     6241 ccgtaggcgt gtattacaac tatagacatc caggatattg gcttttcctc caaacgcttg
     6301 tagcattttt gttgccatat attcataatt tttacttgct aaccctaact ctgtttcttc
     6361 taaggttact tcattgccaa actctgcctt agcttcacgt cctaatgtct gaatgtttct
     6421 cttattgatc aaataataaa aagtaacgta atacaaggca aaaaagaaaa ttccgattgg
     6481 aagaatcaaa atagcattgg tagcgattcg aaaattgatg agataatcaa caatacatgc
     6541 tccgaatgta aaaccaattc gtatattgaa taagtaacag acaatccctg ctaaaccggc
     6601 aaaaagtgca tggatcacgt acaacatagg agaaagaaac atgaaactaa attcaacagg
     6661 ctcagtgata tttgcaataa atgaagttcc tgcaccgctt cccatgattc ctttgaccat
     6721 ctctttattt tctttataag ctgctttata gatagctaga catatagctg gtataccaaa
     6781 catcattact acaaaaaaaa ctgataaaaa cagtccagcc tgaggatctc cgttgataaa
     6841 gcgtgtcatc tctcctctga aaacttcctt gctaggagat gtatagcttc ctaaatcgta
     6901 ataaatatac gtattcaaca catggtgcag tccaaagggg atcagcaaac gattcaaaaa
     6961 cataaatatc cccacaccta atgctcccat atcaacgagc agttttgcaa aattatcaat
     7021 cccaatttgg atgagcggcc agataaagcc caaaaaaaca gctgcgattg tttgtagaat
     7081 catcaccata gtcaatggga atttttcgcc cgcaaaataa gaaaaaacta ctggtaattt
     7141 ttgttcttta aagcgattat acgtccatgc cgctactaaa ccggaaataa ttccaccaaa
     7201 aatccccata ttcacttctg ggtccataat ttctaagcct tttttcaagg tagcaatcgc
     7261 taaaaaacca gtaagtgcag gtattccttt gtccttattt ttgacaaaac cgattgcaac
     7321 tcctatagca aataagatat ccaagtttcc aaaaacggca tttccagctg caccaattac
     7381 tgggatgttc agcatatcgt cagctgataa acgcatgatc aatcccgcta atggcatcgc
     7441 tgcaactgga agtagcatcg ctcttccaag cttctgcaca tttgatttaa cattcatttt
     7501 tattcctcct ctattaaata tcctcatatg ttactaactc aatcaaagga tcatttttca
     7561 ctttttgagc aaatgctgaa tgaattaaaa attcgacttg gtgggtacga ggatacacaa
     7621 aacttgaacc atttaacaat tcctgatcca aatacgctgc gtgacacatg acttcgatcg
     7681 ttttatcttc tctcaatatc atgagtaaac ttgccaaata ttcttccaca ctcgtattga
     7741 tttgtcgttt tttttcatca gtttctccta cgacatcgaa acaaggctca aaataatcga
     7801 catgacgaac ttcatctttt cgttcaaagt tgctccgaac cggcaaatca tatttttttg
     7861 ctaatgtaac aactacctcc tgattttcat ccaaagtatg agtatgatga tgactgtcta
     7921 aatgagttgg tatgatcccc gcatgataaa ttttttgaat ctgagcattc cattcctgat
     7981 acagttgatc tttgtcaatg ttaaatggat gctgataata agataaggat ctgaactctc
     8041 cgctttcgat aagagtatcg acagttttga ggagtggttt gccacacgtc aatgttaaat
     8101 ggactcctac acctagccta ggcatttcct ttcttagttt cacagcatgc tcgaaacccg
     8161 gcatatttgc catcaatgta gttgatgtca aaattcctcg ctgataagca tccataatcc
     8221 cataattcac accatggcta tagccaaaat catccgaatt aaaaatcact tttcccattt
     8281 cattctcctt cttttttcta ctaaaatagg cgcaaccaaa taatcatagt ccatttacta
     8341 tgtgtcattt ggttgcgcct atagatagtt acgcccttat tttaatagat tcttgctaga
     8401 tgaattgcca aatagatgat ttcattttct tgaatggcaa gttgaaattg ctgttcgatg
     8461 atatctttaa tctttagtgc tgtacaatac gctttgctat gtttaaatgt ttcagtaaaa
     8521 aaaccaatat tttctactgg cgtttcttcc tgataaatca agcgtttaac caggaatttg
     8581 atatgaacca ttaagcgatg ataagtcagt gattctttat cataaacgtt tgaataggat
     8641 ttttctagat gggacaaaat cgtggtcgtc atttctacta tttttaagct ctttaaacta
     8701 ttaggctctc ctagctcagc attaatgata tgcatagtga tgaatcctac ttcatttgac
     8761 cctaactcgg tttgatatcg atgattgatt cgctcgacca cttctactgc tatatcatat
     8821 tcatttggat aaaggaatct catttctggc aacataattg ctgtgacatc tctatcttct
     8881 ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg taatatagat atttgaatct
     8941 aatggagagg agatcctagc tgttatcagt tcaatcactt cttccgcaaa atccataaat
     9001 tcgattggaa tctgcgagac tatctcactg aatcgtttga tcttttcttt agaatctaat
     9061 acaaataatt tgtcagcatc ctcaacatca aatggcttac tcactttagc agaaaagcca
     9121 atccctttcc ccatcgcaat tttttctatt ccatcgtcat cgatcaatac agcattatga
     9181 ttcagctttt tcatcacata caccgttaat tcacctcctc tatatgataa aacaaaacaa
     9241 cttgcagact ccttcataca tatttttgtc tccaaacaat ttttaaagac aaaaaacgac
     9301 ctaagaaaga accctttctt aggtcgtgcc taattgaata gtagcaccct acataattta
     9361 caaaaatagt ttaacacata ataaaagcgt tatcaatatc ttttttactc gttttatgat
     9421 aatacttgta tcgaattgat ttggaggtaa ttacataaaa acaaaaaaaa tatagcaact
     9481 ctctataaga aagttgctat attgataatt ataccggcgg ccggggtcga accggcacgt
     9541 cctcaacgga cactggattt tgagtccagc gcgtctgcca attccgccac gccggcaaat
     9601 ttaaaaataa taactggggt agctggattc gaaccaacgc atgagggagt caaagtccct
     9661 tgccttaccg cttggctata ccccaagaat aaagggcgaa tgatgggaat cgaacccacg
     9721 aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat cataaaagat
     9781 gttatttaat ttctcaataa aaaactaaga tgaagacatc ttagtcaata ccggcggccg
     9841 gggtcgaacc ggcacgccct caacgggcac tggattttga gtccagcgcg tctgccaatt
     9901 ccgccacgcc ggcaagttga tttattagta ctaaggcggt aaccggattt gaaccggtga
     9961 tgaaggtttt gcagacctct gccttaccac ttggctatac cgccattata ctattatcaa
    10021 tctataaaat aaagaataaa ttatagaaac tggggtagct ggattcgaac caacgcatga
    10081 gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaaa agggcgaatg
    10141 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
    10201 ttcgccatgg caggggcagc aggaattgaa cccacactaa cggttttgga gaccgctgtt
    10261 ctacctttaa actatgcccc tataaaatgg aggagagtgg attcgaacca ctgaacccta
    10321 aggaacggat ttacagtccg tcgcgtttag ccacttcgct actcctccat ggtggcgcgg
    10381 gacagaatcg aactgccgac acacggagct tcaatccgtt gctctaccaa ctgagctacc
    10441 gcgccatatt ttaaaattat taattgaata acaagaacga cggttccgac gggaatcgaa
    10501 cccgcgatct cctgcgtgac aggcaggcat gttaacccct acaccacgga accagttcat
    10561 tatggaggtt aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag
    10621 ctgagctaaa cctccaacat tcaattaaaa tgacccgtac gggattcgaa cccgtgttac
    10681 cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaat ttgctacgga
    10741 gagtaaggga ttcgaaccct tgagacagtt tggcaccatc tacatgattt ccaatcatgc
    10801 tccttcggcc tctcggacaa ctctccagag tgaaagaagc tcaatactct ttctcaaaat
    10861 gagaactccg gcagtaggac tcgaacctac
//
LOCUS       DACDSR010000087        10681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDSR010000087 DACDSR010000000
VERSION     DACDSR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.087"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWK15_002269"
     CDS             23..646
                     /locus_tag="IWK15_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547815.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWK15_002270"
     CDS             1096..2145
                     /locus_tag="IWK15_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9547816.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWK15_002271"
     CDS             2142..3023
                     /locus_tag="IWK15_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547817.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWK15_002272"
     CDS             3026..3796
                     /locus_tag="IWK15_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9547818.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWK15_002273"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWK15_002273"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9547819.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWK15_002274"
     CDS             5107..6270
                     /locus_tag="IWK15_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9547820.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWK15_002275"
     CDS             6387..6797
                     /locus_tag="IWK15_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9547821.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWK15_002276"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWK15_002276"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9547822.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWK15_002277"
     CDS             9337..9654
                     /locus_tag="IWK15_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547823.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWK15_002278"
     CDS             9814..10050
                     /locus_tag="IWK15_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9547824.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWK15_002279"
     CDS             10047..10445
                     /locus_tag="IWK15_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9547825.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3730 a         1775 c         2255 g         2921 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 t
//
LOCUS       DACDSR010000088        10532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDSR010000088 DACDSR010000000
VERSION     DACDSR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.088"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWK15_002280"
     CDS             complement(25..144)
                     /locus_tag="IWK15_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9547826.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWK15_002281"
     CDS             complement(159..1343)
                     /locus_tag="IWK15_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9547827.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWK15_002282"
     CDS             complement(1599..1853)
                     /locus_tag="IWK15_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547828.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWK15_002283"
     CDS             complement(1878..3020)
                     /locus_tag="IWK15_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9547829.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWK15_002284"
     CDS             complement(3080..4432)
                     /locus_tag="IWK15_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9547830.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWK15_002285"
     CDS             complement(4503..4847)
                     /locus_tag="IWK15_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9547831.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWK15_002286"
     CDS             complement(4857..5231)
                     /locus_tag="IWK15_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9547832.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWK15_002287"
     CDS             complement(5244..5558)
                     /locus_tag="IWK15_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9547833.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK15_002288"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK15_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWK15_002289"
     CDS             complement(8976..9638)
                     /locus_tag="IWK15_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547834.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IWK15_002290"
     CDS             10255..10485
                     /locus_tag="IWK15_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547835.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DACDSR010000089        10412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDSR010000089 DACDSR010000000
VERSION     DACDSR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.089"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            129..2825
                     /locus_tag="IWK15_002291"
     CDS             129..2825
                     /locus_tag="IWK15_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9547836.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2902..3390
                     /locus_tag="IWK15_002292"
     CDS             2902..3390
                     /locus_tag="IWK15_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9547837.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3402..3896
                     /locus_tag="IWK15_002293"
     CDS             3402..3896
                     /locus_tag="IWK15_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9547838.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3917..4726
                     /locus_tag="IWK15_002294"
     CDS             3917..4726
                     /locus_tag="IWK15_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9547839.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4723..5544
                     /locus_tag="IWK15_002295"
     CDS             4723..5544
                     /locus_tag="IWK15_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9547840.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5595..7694
                     /locus_tag="IWK15_002296"
     CDS             5595..7694
                     /locus_tag="IWK15_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9547841.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7691..8809
                     /locus_tag="IWK15_002297"
     CDS             7691..8809
                     /locus_tag="IWK15_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9547842.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8848..9726
                     /locus_tag="IWK15_002298"
     CDS             8848..9726
                     /locus_tag="IWK15_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9547843.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9917..10069
                     /locus_tag="IWK15_002299"
     CDS             9917..10069
                     /locus_tag="IWK15_002299"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547844.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3214 a         1694 c         2307 g         3197 t
ORIGIN      
        1 atccagttca atatttgtag ctatgaaaaa acataaaaat catattagat aaaaactaaa
       61 ttggtagata aattattttg tcagatatct gaggcaaata ttcgactatt tgtgtttcaa
      121 agtaaaaatt ggaatacaaa tatagaatag acatattgca gccgatagtt tattatgctt
      181 ttagacacat taaactggag gaaaaaatga aaaagagcgc taaagatcga gtttttgatg
      241 agctgatacg actaacaggg aatatgtctt ctgtcactgc cgagaaaatt tcgaagaatc
      301 taggtatctc tcgtcagaat gccagccatt atttgacgag attagtggaa gataaaaagg
      361 tcgagaaatt accgggaaag cctgttttgt ggaagccttt agatgaatat gcggttgtag
      421 ataatacgga gcaaataaac gaagcctttc attctgtagt tggtcatgac ggaagtctga
      481 gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc gcctaacgga ctcagtgtat
      541 taataaatgg agccacgggt gttggaaaaa gttttttagc cacaaaaatt tttgaatacg
      601 ctatccatga gcaaattatt gaaaaagatg caccatttgc tatcttaaac tgtgccgatt
      661 atgcggacaa tcccgaactg ctttcggcaa cactgtttgg atacaagaag ggggccttta
      721 cgggggctga aaaggatacc gatggtttat tggcaacggc caacaacggc tatctttttc
      781 tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa gctctttcta tttattgata
      841 ctggaaacta tcgtccagtc ggtgaaaatg taaattggca ttctgctaag gttcgtttta
      901 ttttcgccac gacggaaaaa ggtgaaaatt atttattaga cacctttgat cggcgtattc
      961 aaattagtgt tatgttgcca acttttgaag atcgccccat tcgagaacga ttagagctga
     1021 ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa agatattatt gtttcaagag
     1081 aagccttgtc tttaatgtta tctcatccct ttagtgggaa tattggtaag ctgaagaata
     1141 tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca agaagaacca ttaacgattg
     1201 ggaaaaatga gatcttgatt cagttaaata tgctggaatc cgaagtagaa agcttgccga
     1261 ttgctagttt attgataaag aaaaatcaaa agataaaaac ggatcgaatc aaaccgtttg
     1321 attatgattc gattttggaa aaaattactc agctgataaa tgaaggtaat ttcaatgaaa
     1381 atttttcttc gataaagaat gaagtacagc atttggtgca tcacattgat aaggattctt
     1441 ttttatcaat gagtagaaaa ttgatgattc agatgtttag aaaggtctgg gtatccgttt
     1501 taggaaaaac gtacggattg acgacgagtg gacctgttgc agaaattatt agcagatcct
     1561 atgtcatttc cccaagctat aagacagact taaccgggat aaatcgtaca ttagctgcaa
     1621 atctacctcg gacgacttac ttatgtaatc agttcttgga gcatttacca ccgctctcag
     1681 aaaaggacga gaactttcta agactgctta ttgtcgtgac tttatctgat tatgtagatg
     1741 aatccatcga gctgaagggc ctgttgcttg ctcacggaga tagtacggct agcagcattc
     1801 aagcagtagt gaatcaatta tgcggcaatt atgtttttga agcgttggat atgccagtag
     1861 agagtaatgt gtcagagatt gtcgaatcga caaggaattt cgtgaaacgg caagtgaata
     1921 cagatagtct agtgctgatc gtagatatgg gctctttaaa tcaaatttac acacagatca
     1981 agaacgattt aaaaggggag ttacttcttc tgaataattt gacgacttcc attgcacttg
     2041 atattggctt gaagatgacg atgaatgcgc catttaaaca gattgcggag gatgcagcag
     2101 acaagtattc aatcaatgtt caatactttg aaggcttttc tcaaagtagc aacattatca
     2161 tttcctgtat gtcgggtttg gggatttcgg acaaattgaa agatgttttt cgtcatcatt
     2221 tatcggatga atccatcgaa gttttcacga aggactacag agaactacgt gaattaatcg
     2281 accaaaatga tgaaacttat tttgacaaga caaaattggt catcacgact tcggatcttc
     2341 cacgttcctt ttcaattcct aatattaatg tttatgacat cttagacggt gatggagcag
     2401 ccaatttgga gcatattttg agtcctgaaa tgaagccggc agaatttaac cgcttgattc
     2461 aggagctagt aaaattcttt tcgttggaag gaattgcgga ccgtttgaac tttctgaacc
     2521 caacgatcgt gatgagtgaa gtggaaacag tcatttctaa atatgaaaat tactatcatt
     2581 tcactttaaa tggccgtgta aaactaaatc tctatatgca tacggcattg atgatggaac
     2641 gtttattctt ggctcggaca gaaaaatctg aagaaccgaa ttatgatttg aaaccagatg
     2701 aagatgaatt ttattatgtt tctagaagta tttttcaatc aatggagatg aaatataatt
     2761 ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt attaggaccg tatattcaaa
     2821 aataaagtgt ctaaaaaagt gtctaatctg ttttttttag gttagacact ttttttaatg
     2881 tgaattaacg tgattaaacc aatgttttta aaagttggca caggaattgc ttatataaaa
     2941 gtgaacagaa agaaaggaat taaccatatg aagaaaattc taatagcgac tcacggtcac
     3001 ttagcttcag gctttcttag cagtattcaa ttactgacgg ggaaggttca agagattacg
     3061 gtgattaatg cttatgtgga tgagtgtgat tttgaacagg agttgaatgt atttgtaaac
     3121 caggtgaacg agcaggatca gatttttgtt tttaccgatt tatttggcgg aagtgtcaat
     3181 cagaagatta ccaaaacctt tttggagaaa gagattgctg taactatttt gtctggcttt
     3241 aatctgccaa ttctgctcga aatagtattg gcaaccaagg aattaacaaa ggaggatatt
     3301 caggaactgg taaataaatg ccaacaggaa ttaaagatga ccattcttga tgaggtacat
     3361 gtagtaaatg aagaggaaga ttttttttag gaggaagaaa aatgattaaa gcagtgcgaa
     3421 ttgatgaacg attaattcat ggacaagtag cgatgacttg gacaaagagt ctaaatttaa
     3481 ccgggttagt agtagcaagc gatgaggcgg ctaccaacga tattcaaaag atgacattga
     3541 agatggctgc accatcgaat gtcaaagtca ttattaaaac agttgatgac gcgattcatt
     3601 tgttaaaaga tccgcgggcg aaggacatgc gcttgatggt tttagtccct aatgtcaaag
     3661 atgcggtaaa agttgcgaaa gctttcccaa cggaaatcga attaatgaac gtggggaatg
     3721 ctgggaaaat gacaccgcca tcggaagata agaaaacctt atcaaaggaa gtgatgttaa
     3781 cgacgtcaga aattcaagac ttaaaagaat tagttgaact ttatccagat accgtttttc
     3841 agcctacacc ggcgatggaa aaacgttcag ctatctctat tttgagtgat ttttagaaaa
     3901 aggaaggaga aaaattatgt atgatgcatt tgtagtcgct ttggccgttt ttattggaat
     3961 tgctggacat gaaatgttcg ggatgtccat gctgagtcgt ccgattgttg ttgctccgtt
     4021 agcaggagcg ttgatggggg atttgcagac ggggttgatt gtcggggcgt cactagaagc
     4081 catctttatg ggtgtcgtca atatcggaat ctctagtacg gcggagcctt ctttggctgc
     4141 tgggctggct acagcgttcg caatcaaaat gggaggaaac gtggatgcga ttattcccat
     4201 tgtctttcca ctagcagtat taggattaca gttaatgaat atgatttttt cttttgtatg
     4261 tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct ggtgatcaaa aaggaatcat
     4321 caatctgcac ttctttatgt ggtttgtcca ctatgcgctt tatgctctta ttccattctt
     4381 tgccgtttta tttggagcag atgttgtcaa aagcgtcttg gataatattc cagaggtcat
     4441 catgaacgga ttaacagtcg ctggaaatct gctgccggca gttgggatgg cgatgctgtt
     4501 acggatgctt tggagtaaaa atattgctgt gtggtttttc tttggggccg ttgttatggc
     4561 gtatctcgag ttaccattga ttgcaattgc cgcagttgga acgattattg caattgttat
     4621 agcccaaaga gatctacaac taaaaaatat atctgttcat gcagttcaaa gcgatgctgc
     4681 tgtaaatgca acgcttgacc aagaagagga ggatttcttt caatgaaatt agctgatgga
     4741 atttctaaag atgagaaaaa attggttcgg aagatgtttt ggcgctcgat gactatgtat
     4801 gcttcggtga atccggtgac gatgggcggc ggtggtttcg cctattctat gatgcccttc
     4861 attaatcatt tctacaaaag tgaagaagat cgagctgcgg cgctggaacg tcatacacag
     4921 tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg ggattgcggc atcgatggag
     4981 aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta tcaacgcgat caaaacgagt
     5041 ttgatgggac cattggctgg aattggcgat tcattgttct ggggagtttg gcgtgtcgtt
     5101 accgcgggac tagcgatcaa cttagccaat gccggaaata ttttggcacc aattatcttt
     5161 ttagttatgt tcaatatacc taattacttg atgcgatact atggcggcgt tttaggctat
     5221 tcattaggct ctaaatatat tgaaaagctg tatgcgaacg ggttaattga aattctgaca
     5281 aaagctgcta gtatcttggg tctgatcatg gtaggtgcaa tgacgatcag tatggtcaaa
     5341 ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac taatgaatgt ccaagaaatc
     5401 ctcgatagta tttttaaagg aattgtaccg ttattggtaa cattgagctg tttcaaatta
     5461 ctagataaaa aagtcggcgt catcaaaatt atttttggaa tggtcgtctt gggaatttta
     5521 ttgtcattac ttggaatcgc atagtaggaa gctgcagcta ctaggctgca gtttttttag
     5581 caaggaggga aaagatgact attaatgaag taatttattc acccaaacac ccatttttta
     5641 atgctgagtt aaacgacgga aatgtgacta cttcaaacga atacagctac tatcggaata
     5701 ttcctgatgt aaagggattg accttcgatg taagtaatca aaagacatta gcaaaccttg
     5761 atgtttgcgg gacgattcaa acgttgacct tttatcgaga aaatttacta actgaggaaa
     5821 aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa ttcgttatcc ttaaaggttg
     5881 aagtaaacgg gattgtctac catttgtcag aatcggatca tcgtattgaa gtcgatgtga
     5941 ttcaagactg tttgcctaga tatattcatc attattcgac gtttaaggtc attgtagtat
     6001 cctttgctcc catactggct aataagcgtt taagtatgct tgttcaacag gtttacattg
     6061 tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga tgtgccttcg tatcaagaga
     6121 agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg aaataaggac acgatcgcac
     6181 aacaggaagt ggcaagcttc gccattgctt ttgtggaccc aaatgctttt gaagaagtaa
     6241 tgacttttca ggaatctgat atcgaaaaat ggctgaagga aacattgaac tattttgctc
     6301 agctatttgg gcagctgtca atgccaaacg atagagttgt ccatctctat cgtcgtgcac
     6361 tttatcaaag cttttctagt ttcggtatga atcgacaggg acaggtcgtc ggcagcaatt
     6421 ggggcagcta tcctgctact aatcgaattt ggaataagga tatgtattac gcatcattgc
     6481 cattcaccat gtttgaaccg gagttatgtc aaaaaacggt tctatggttt gatcaatatg
     6541 gcgttaagtt tcctggttca aaatttacag gaggtatcaa tcattctctg agtaattcgt
     6601 tatccagtgc gttactggct agtctttatt ttgaacatac tggggataca aaattctttg
     6661 agcggtgtcc gcaggttcta ctgaatgctt caagaattat tgaggacata ttagctcaac
     6721 gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt atcagacgcc tttgcacttg
     6781 gaaagtatca taccggcagc aatctttgtc tttggaaggc ctgtattgga cttgcagaaa
     6841 tttttgaagc cctagaacaa ctgagtctta gcaagcgata caaaaacatt gcctgtaaac
     6901 taaaggaatc aatttgtaaa caaatgacta tcgatggtcc ttttggtgag caatttttgg
     6961 agggaatcgg tgatgaggag aaaacaacct attcagtgaa gcactatcaa aaaccgattc
     7021 ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga tggaaaaatt aatttattga
     7081 tgcatgatgg agaagaatca gacactacgt tgatgccttt ttatcaattt ttagataata
     7141 aagatcattt atatcagaac accatggcct tttctgctag ttctttcaat ccaacctata
     7201 gcgaggaaat caaaggcatc acttggggat tagaatccgg cgccactttt cctggcttta
     7261 tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt cgtaacccga ttagaagaac
     7321 taatttgttt ggcagattta gacggttcgt ggtggtggtg gccgtatcgc ttagaggctc
     7381 aacaaggaga agtcgtgcga gactttggct gcggcaaatg cggctgggca tcgggtcttt
     7441 ttgtcagcct aatgatcagt caatatttcg gattgaaatt caaaaaagga gtattgcaaa
     7501 ttgatcctgt tgataattta acgttttctt ggaagaattt gaagttcggt caagccttca
     7561 tttcgattga atgtacgcaa tccgagttga gtatttcaaa tttgtcagaa aagccattga
     7621 aaataagtga tacgaaaaaa attcttcacg tagaaaaggg aaaaacgatt cacattcaaa
     7681 ggagaaaaag atgagtatac taaaaaatat tacccaattt aaaaaaatcc agagtcgagc
     7741 aatcactccc gaaaatccaa caggcgagaa aggtaaagct gcgatgttgc caagcgagct
     7801 aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact tctggtgaag aaactgtgat
     7861 tgcggatatt actggaacgg gaataattcg tcatatgtgg atgacgattc gtgatcgaac
     7921 agaatttggc agttttgttt tacgtgattt aatcttaaga atttattggg atgggtcgga
     7981 tagccctgct gttgaaagtc ctttagggga tttcttttgt aatgggtttg gagagcgttg
     8041 cgaggtgaac tcgttgccga tagtggttaa tcctacaggg ggctttaatt cctatttcga
     8101 aatgcctttc caaacatccg ctaaaattac gatcgagaat ttacatccga aggatatgtc
     8161 ctcatttttt tatacgataa attacgcttt ggtggacgaa ttggaagaaa atagcctata
     8221 ttttcacgca aattggcatc gggaacgcat taccaaatta caggaggatt ataagttaat
     8281 tgatcgatta aagggtcaag gctattacgt gggtacttac cttgcgctta cctgcctaga
     8341 acgttattgg tggggcgaag gcgagtttaa attctacatt gatggggata gtgagtatcc
     8401 gaccgtaacc tcaacaggtt cagaagatta ttttggcggg gcgtgggcct ttcatgagta
     8461 cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca gcctttttag ggtatcctta
     8521 ccaatcaaat agagatgcca caagagatcg ttttgaaacg ggaaaattga atgccgttca
     8581 tgcctttggt gacgacgcaa tgccacgaca tggtctttat cgttggcatc ttcaagaccc
     8641 catctgtttt accgaagatt tatcggtcac tttacaacaa atcggcaacg atgatgttcg
     8701 cttatttgag cggcaagacg atgttgctag tgtcgcttac tggtattcaa cagaagcttc
     8761 aggaaatact aaacgtcatt tcactgatcg agaacgcttg ccgcgttaat gaagtgtttt
     8821 tcacatattc gaagggagga acctatgatg atatatgggg caattgaagc tggagggact
     8881 aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt tggaaagagt cagttttccc
     8941 acaacaactc cagttgaaac aatgaagcag gtcattgatt tttttaaacc ttttaaggaa
     9001 caattggcag gcatcggggt cggctgcttt ggtccaattg atattcaatg caaatcaacg
     9061 agtttcggtc atatcacatc aacaccaaaa cttgcttggc aaaatttcaa ttttgtagga
     9121 acgctaaagc aatatttcaa tattccgatt gaatggacaa cagatgtcaa tgcggcttgc
     9181 tatggtgaat atgttgctgg ttctggcaaa gggttagcag gtgttgcata cttcactgtg
     9241 ggaacaggta tcggaggtgg tgccttgttc aatgggactt tcgtggaagg gttcagtcat
     9301 ccggaaatgg ggcatatgtt gatcaagcga catccaaagg acaatttttc aggaaactgt
     9361 cccttccacc aagattgttt agaggggatg gcagcaggac cagcgattga aaaacggttg
     9421 ggtgtaaaag gccagaattt attggctgac gattcgtttt ggcagattga agccttttac
     9481 cttgcccaat gtgcatacaa tacaacactg atgttttcgc cagatagaat tatttttggc
     9541 ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc aggataaatt tgttgagctt
     9601 ataaatggat atgttgaaat accaccaatt gatagctaca ttattacgcc cgaattaggt
     9661 gataatgccg gaataatcgg cggattagca ttggccagaa aagcggtaag aaataagcaa
     9721 ccataattaa tgaatgtttc attgatactc atgtcaaaag tatttaaccg agtaatcaaa
     9781 tgatcaaatg aagttggata aagcgaaaat gaattcaaat aaaaaggctc taagtcaaat
     9841 cgtgttgttt tcattaaatc actgaaagga aatgcttctg cgtttccttt tttctttttg
     9901 ttgtgcgctg tataccttga ggatattcac tggtggcgta cacttaaata gtaatatatt
     9961 gtgttacttt atgtctgtgg taatagttat agttgcttac caatgtatgt tcatttttcg
    10021 aaatgttgac ttcataatcc ttggcttaat gtttgaattt ttttggtgat agattttttc
    10081 agccgtataa aataaatcgt aattctatta tagagagttg tgattataaa aagatagcaa
    10141 gaaaaaaaaa gaaagtttct atacatttta aatattttat tcctatttct attgctaaaa
    10201 aagtagaaat tctatattta cctttaactc tcgcttctct tgcaaatgta gttagcattt
    10261 taatcgataa taaactcagg tagttatatt accttgaaat gattgataat tttgtcactt
    10321 ctattttttt tgcaataaat tttttatttt agtgaaaaat aagaaactat ttctttgaag
    10381 gcgataagtt agaaaaatat tattgtagtc tt
//
LOCUS       DACDSR010000090        10333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDSR010000090 DACDSR010000000
VERSION     DACDSR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.090"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IWK15_002300"
     CDS             257..532
                     /locus_tag="IWK15_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547845.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="IWK15_002301"
     CDS             545..2617
                     /locus_tag="IWK15_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9547846.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="IWK15_002302"
     CDS             2782..4668
                     /locus_tag="IWK15_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9547847.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="IWK15_002303"
     CDS             4680..5627
                     /locus_tag="IWK15_002303"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9547848.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="IWK15_002304"
     CDS             5647..6174
                     /locus_tag="IWK15_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9547849.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="IWK15_002305"
     CDS             complement(6237..6890)
                     /locus_tag="IWK15_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9547850.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="IWK15_002306"
     CDS             7022..7864
                     /locus_tag="IWK15_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9547851.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="IWK15_002307"
     CDS             8022..8918
                     /locus_tag="IWK15_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9547852.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="IWK15_002308"
     CDS             8921..9634
                     /locus_tag="IWK15_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9547853.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="IWK15_002309"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="IWK15_002309"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9547854.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..>10333
                     /locus_tag="IWK15_002310"
     CDS             10169..>10333
                     /locus_tag="IWK15_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9547855.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYL"
BASE COUNT         3581 a         1765 c         2141 g         2846 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttg
//
LOCUS       DACDSR010000091        10196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDSR010000091 DACDSR010000000
VERSION     DACDSR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.091"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            121..627
                     /locus_tag="IWK15_002311"
     CDS             121..627
                     /locus_tag="IWK15_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9547856.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="IWK15_002312"
     CDS             644..1543
                     /locus_tag="IWK15_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9547857.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="IWK15_002313"
     CDS             1630..2514
                     /locus_tag="IWK15_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9547858.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="IWK15_002314"
     tRNA            complement(2706..2777)
                     /locus_tag="IWK15_002314"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="IWK15_002315"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="IWK15_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9547859.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4374..5411
                     /locus_tag="IWK15_002316"
     CDS             4374..5411
                     /locus_tag="IWK15_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9547860.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="IWK15_002317"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="IWK15_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9547861.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="IWK15_002318"
     CDS             6563..7753
                     /locus_tag="IWK15_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9547862.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="IWK15_002319"
     CDS             7857..8612
                     /locus_tag="IWK15_002319"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9547863.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="IWK15_002320"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="IWK15_002320"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9547864.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3566 a         1886 c         2112 g         2632 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc ggctctttgt caataaggac
    10141 tgatgagttg tgcaaaatca aatctgagtc agaatgaacc acattctggc tcagat
//
LOCUS       DACDSR010000092        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDSR010000092 DACDSR010000000
VERSION     DACDSR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.092"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWK15_002321"
     CDS             complement(247..759)
                     /locus_tag="IWK15_002321"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9547865.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWK15_002322"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWK15_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9547866.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWK15_002323"
     CDS             complement(3212..3646)
                     /locus_tag="IWK15_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9547867.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWK15_002324"
     CDS             complement(3656..4444)
                     /locus_tag="IWK15_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9547868.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWK15_002325"
     CDS             complement(4441..5382)
                     /locus_tag="IWK15_002325"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9547869.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWK15_002326"
     CDS             complement(5559..6146)
                     /locus_tag="IWK15_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9547870.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWK15_002327"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWK15_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9547871.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWK15_002328"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWK15_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9547872.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWK15_002329"
     CDS             complement(9063..9998)
                     /locus_tag="IWK15_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9547873.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDSR010000093         9758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDSR010000093 DACDSR010000000
VERSION     DACDSR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.094"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..497
                     /locus_tag="IWK15_002330"
     CDS             18..497
                     /locus_tag="IWK15_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9547874.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            590..1752
                     /locus_tag="IWK15_002331"
     CDS             join(590..866,866..1752)
                     /locus_tag="IWK15_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9547875.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2032..3012
                     /locus_tag="IWK15_002332"
     CDS             2032..3012
                     /locus_tag="IWK15_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9547876.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3134..4552
                     /locus_tag="IWK15_002333"
     CDS             3134..4552
                     /locus_tag="IWK15_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9547877.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4623..6092
                     /locus_tag="IWK15_002334"
     CDS             4623..6092
                     /locus_tag="IWK15_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9547878.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6102..6569
                     /locus_tag="IWK15_002335"
     CDS             6102..6569
                     /locus_tag="IWK15_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9547879.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6605..7477
                     /locus_tag="IWK15_002336"
     CDS             6605..7477
                     /locus_tag="IWK15_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9547880.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7558..>9758
                     /locus_tag="IWK15_002337"
     CDS             7558..>9758
                     /locus_tag="IWK15_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9547881.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFT"
BASE COUNT         3393 a         1419 c         1957 g         2989 t
ORIGIN      
        1 tagaaagagg tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca
       61 tcaaagtaaa taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc
      121 taaaactcag tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta
      181 gtattacgga attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc
      241 ccactttaga gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta
      301 aacttgtatc gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg
      361 aagagcttta tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa
      421 ccattaaaca gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga
      481 caagaaagga agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca
      541 gaaaaaataa ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg
      601 tcaacgtaga acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg
      661 aaagccccga gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg
      721 ggtcaagcaa gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca
      781 agtagaacta attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt
      841 aaagcaagcg gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa
      901 tactcaatat cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa
      961 gccaaaccag ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc
     1021 aatcgaaaag cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa
     1081 gtgagtcaac gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca
     1141 ttggctcatt tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta
     1201 gacggaacct tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt
     1261 cgtgttggga agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc
     1321 atcggctatt catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag
     1381 attccgtact cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat
     1441 aaccagacga ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa
     1501 ggctgcccct atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc
     1561 gttcatcaat accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc
     1621 cattggtgga ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt
     1681 cgacagcaga gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc
     1741 gaggcacctt aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg
     1801 ttgtcaaggg cagtcggaac accagcagca agcttacgca ataccttata atttttgtca
     1861 aaaaaagtgt tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg
     1921 atattatata tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat
     1981 gtcgaatagt cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa
     2041 attggaatta gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta
     2101 ttgaatgatc gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa
     2161 aaattagaat actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc
     2221 gtaggtttga tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc
     2281 gagttcgagc tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca
     2341 acaaaagaaa aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta
     2401 ggaacgcaca ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt
     2461 gatagaagtc ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa
     2521 ttagctactc aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat
     2581 agtaagataa agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa
     2641 ttcatgctgg aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat
     2701 gaaataatta agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat
     2761 gatttattgg cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa
     2821 gaggttaaag tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta
     2881 tcaactattc aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat
     2941 atgattaagg gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa
     3001 aatacagcat aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga
     3061 aacgggttga catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca
     3121 ctggaggaag aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca
     3181 acctaaggat gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat
     3241 ttatcatcaa cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt
     3301 agcaactacg aaagattttg ttcattttga agactttggg gaatctcttt taagagggga
     3361 tgacaatgag agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt
     3421 tcacgcattt tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt
     3481 gttgcttcat gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga
     3541 gttagaacca caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga
     3601 tgatgaaaaa gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct
     3661 acaaaccgga cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg
     3721 tgatttttgg gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg
     3781 tgactggtgg tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat
     3841 gagtcaaagt ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc
     3901 ttattatgca ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc
     3961 aacaaaagat caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca
     4021 tgaagtctat caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa
     4081 tgcattcata aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga
     4141 agaagtaacg cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt
     4201 tgatgagaat cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc
     4261 ttacgaatat cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta
     4321 cccttggttt caatatatgg ataaaggact tattagacct atatctttaa agcctaacca
     4381 atcttataaa ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat
     4441 cgctcttaac gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa
     4501 tggatcaatg actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc
     4561 aatcatgtgg caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat
     4621 gaatggacta caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata
     4681 tctcaagcgc tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag
     4741 cgaatatgga aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac
     4801 agttacaagt aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat
     4861 tgacaggagc taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag
     4921 gaaatagact tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag
     4981 cttttattgg ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt
     5041 ttggaatgga tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc
     5101 tgggtattat tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa
     5161 aacgttttgg cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag
     5221 gactagccaa tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat
     5281 ttggattaag cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt
     5341 cttggttttt agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt
     5401 acattttagt cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg
     5461 gtccattggg gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga
     5521 agatgggtgc ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag
     5581 gaacacaaca tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt
     5641 atatccaacc tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt
     5701 atttactctt taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa
     5761 cactggtagg tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc
     5821 cttttgtttg ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg
     5881 taaaagccat tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc
     5941 ttccttatat atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt
     6001 atgataaata taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg
     6061 actcaaaaaa attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa
     6121 aaaatgaaat aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa
     6181 gtgatccagt cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg
     6241 aaacgattgt atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct
     6301 taggaataaa aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg
     6361 agctaaatgg tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg
     6421 atattcttat gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca
     6481 ttgttgtttt tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac
     6541 aaggacaaga attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa
     6601 atgtatggtt tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga
     6661 taaagatttg aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat
     6721 gaatttagtt ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg
     6781 atcgttcggt cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac
     6841 gccaaaactt gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat
     6901 tccaactgct tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa
     6961 tggcagaggc ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc
     7021 tatccaaaac ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt
     7081 aaaacgacac aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga
     7141 gggcttagct gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac
     7201 tgaaaatgac gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac
     7261 aacattgatg ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca
     7321 cttaaaaaag aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc
     7381 cgatgttgac aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg
     7441 tttagcttta gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac
     7501 tatttgtttg gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg
     7561 aaaaaaaaaa tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat
     7621 ttttctgctc aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat
     7681 ttgaatcagt tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg
     7741 ttcaagtata atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata
     7801 tctttgaaat ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc
     7861 ccattaataa taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat
     7921 cagtatgctc ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt
     7981 gcagattatg caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga
     8041 gcctttactg gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac
     8101 ctttttttag atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt
     8161 atggattctc aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt
     8221 cgccttatct tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga
     8281 cgcatcccag ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt
     8341 caattattat ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt
     8401 tcgattgaac tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg
     8461 aggaatgaaa tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca
     8521 atttatttag gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat
     8581 ttaaaaaaaa tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat
     8641 cacatttcaa ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca
     8701 ggttcttctg agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt
     8761 gttaataaaa tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg
     8821 gcggatatca gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt
     8881 ttgctgtcta agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat
     8941 gagatttgga gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata
     9001 ctttttcttg taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg
     9061 cacggtaaaa taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat
     9121 gtttatgagg ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc
     9181 aacaattttt gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga
     9241 tcattggaac aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta
     9301 aacaatgttt ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca
     9361 attagtcatt tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa
     9421 ggattatcac aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa
     9481 aaagttaagg atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat
     9541 tttaacgcat taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt
     9601 tgtgttcttt caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat
     9661 ctaattaatg ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt
     9721 gagttctgct taaatgaatt agttaaactc tttactat
//
LOCUS       DACDSR010000094         9526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDSR010000094 DACDSR010000000
VERSION     DACDSR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.095"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..663)
                     /locus_tag="IWK15_002338"
     CDS             complement(16..663)
                     /locus_tag="IWK15_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9547882.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(747..2174)
                     /gene="uxaC"
                     /locus_tag="IWK15_002339"
     CDS             complement(747..2174)
                     /gene="uxaC"
                     /locus_tag="IWK15_002339"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9547883.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2248..3327)
                     /gene="uxuA"
                     /locus_tag="IWK15_002340"
     CDS             complement(2248..3327)
                     /gene="uxuA"
                     /locus_tag="IWK15_002340"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9547884.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3330..4961)
                     /locus_tag="IWK15_002341"
     CDS             complement(3330..4961)
                     /locus_tag="IWK15_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9547885.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5278..5949
                     /locus_tag="IWK15_002342"
     CDS             5278..5949
                     /locus_tag="IWK15_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9547886.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(6785..7741)
                     /locus_tag="IWK15_002343"
     CDS             complement(6785..7741)
                     /locus_tag="IWK15_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547887.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(7850..8095)
                     /locus_tag="IWK15_002344"
                     /pseudo
     CDS             complement(7850..8095)
                     /locus_tag="IWK15_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8240..8662)
                     /locus_tag="IWK15_002345"
     CDS             complement(8240..8662)
                     /locus_tag="IWK15_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547888.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(8801..9231)
                     /locus_tag="IWK15_002346"
                     /pseudo
     CDS             complement(8801..9231)
                     /locus_tag="IWK15_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         2972 a         1946 c         1399 g         3209 t
ORIGIN      
        1 tatttatttt ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc
       61 tattcgagtt acttcttcaa aatcaccttt ttcaacagga gccaacaaat tcccacctac
      121 accaactgca acaacgcctg cttcaaacca ctcttctaag ttatctagac ttactccgcc
      181 agtaggcatt atatttacat atggtaatgg agcttgatac gtttttacaa cagatggacc
      241 aaaagcagaa cctggaaata gtttaatgat ttctactcca gcggtaagtg cctcctttat
      301 ctcagtaatt gtcatacaac ctggtaaata aggaatttga tataaattac aaatctctgc
      361 tgtttgtcga tcaaaagatg gacttacgat atattctgct ccagccataa ttgcaatacg
      421 agcagtaaac gcatctaaaa ctgtaccggc tccaattatt acatcttttt gatcagcata
      481 ttttttagca atttctttga ttatttcttc agcattcgga actgtaaaag ttagttcaat
      541 ccctttaatt ccacccgcag taattgcatt tattgcttta atggcctcat ctttattttt
      601 tccacgtaca accgcaatga ctccagccgc ttctaactta cttaaaattt ctactttttt
      661 catatctatc tccttctttt atggatacat actctagcta gttgtactaa cttacaataa
      721 ataaatttaa gcttttattt tttgtattat tttctacttt cgatttcaaa aaaattaaaa
      781 taattatacg cattacgata acaaatattc tccactatct gactaagata catttcatca
      841 tctggaattc gacctgcttt aacccactcg cttagtaatt gacataatac tcgacggaaa
      901 tactcatggc gaggatagct tagaaaactt cgagaatcag tcaacatacc tgtaaaattc
      961 gacaacaaac tttgttgcgc catcgtagtc aactgttgac gcattccttc agctgtatca
     1021 ttgaaccacc aagcacatcc caattggatt ttttgaatca tacccccttg gaagctttgc
     1081 attgctgttg ctagtggaag ccaatcggtt ggatttaatg aatataaaat tgtttttggt
     1141 aagcacccac tcttttcagc agtgtcgaac aatgctacaa cattttgtgt caaactagct
     1201 tgatccccca tcgaatcacc accgacatct actccaattt gttcctttaa tatacttgaa
     1261 ttatttctta aagcatttaa atgcatttgc atcgtccaac catatcttgt atactcagac
     1321 atcaaatgta attgaatacc tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt
     1381 tttcctgcta aagcttttat taacagattg tcgagtattt tttcatctgc tatttcataa
     1441 aaataagtat tcaaaccgtg atctgctaat cgacagccaa tttgatgaaa ataatccact
     1501 cgttggctca atgcctgctt caacccatta aagtttttga ttggtatacc cgctgcttga
     1561 ctaagtttta aaatatagtt cgaaaatcct gatgatttga tactccagac cttatctgga
     1621 cggaaagtag gtaatacctt gaaagaatta tcctgtttag aaagctcagt atgatatcgc
     1681 aagtcatctg taggatcatc cgttgtacaa atggctttca catccatcat cttcagcaat
     1741 tcttttgggc gataggaatc cgtttggatt ttttcgttgg cacgtcgcca aatttctggt
     1801 gcattcttct cagaaattgt caaatcaata tcaaagtaac gttgtaattc caaatgagac
     1861 cattcaaaaa taggattacc tggcgccttc tccactgttt ttacaaaagc taaaaatttt
     1921 tcataatcat tacctgcacc actgatcaac tcttcttcta tcccattagc tcgcatcaat
     1981 cgccatttat aatgatctcc agctccttta tcatttaacc atacctgagt caaattcttg
     2041 tattgcttat tttctagcaa acatgctggg tctaaatgac aatgataatc gatgattggt
     2101 tggtgttctg caaatttatg aaaaagtctt ttagctattt cattctctaa taaaaagttc
     2161 ttatctaaaa acatatttaa gcactccttt ccttccctta gtttttagaa gagaaaattt
     2221 ttcttctaaa tagactccat tatataatta tttactcgcc cccttttcaa gcgcttccca
     2281 aataccattt aaataaactg cacctagagc tcggtcatat aacccataac caggtctacc
     2341 ggtttctccc caaatcatcc gaccatgatc tggacgaata tagccattaa atccattatc
     2401 gtacattgtt tttaatatct tatacatatc tagagaacca tattctgatt tatgcggcgc
     2461 ttcataaaaa tcgagtccat tatcacttaa atatttgata ttacgaacat gtgcaaaagg
     2521 aatccgatcc cgttgacaaa attcagatag aatttcatag acatcattat ctggtcgttc
     2581 tgcaatactt ccagtacata acgtaatacc attagaagga gaatccacta cattacacaa
     2641 ccattctaaa tcgtcacgat ttttaacaat acgaggtaat ccgaaaatag aatatggtgg
     2701 atcatctgga tgaactgcca ttttaatccc aacttcttca catgtcggga taattgcttt
     2761 gagaaaatat gcaaagttct ctcgcaattt cgcttcatca acttctttat aggcttctaa
     2821 taattgcttg atttgtgata accgatgagg ttcccaaccc ggcaattcaa aattattagt
     2881 agaattttct acttttctaa taatttcctc tggatcagta ggaatatttt ttgaaatata
     2941 ggctaacgta gaagacccat catctaaatg ataatccaaa tctgatttta cccaatcaaa
     3001 gactggcata aaattataac agataacctc aatcccaaat tctgccaaat tgcgaatcgt
     3061 ctgtttataa ttctcgatgt attgatctcg tgttggcaaa ccaattttga tatcatcatg
     3121 aacattaaca ctctcaataa ctttcaaggt aaggccattg gcttcgactt catcttttaa
     3181 agctttaatt cgttctctcg gccagacctc tccggctggt atatccatta atgttccaac
     3241 aattcctttc attgctggga tctgacgaat ttcttgtagt gagatttttt cttcatttgc
     3301 cccataccat ctaaaagtca tttccatagt tactttcctc caacttctgt tatatattta
     3361 ttgattgttt tacgaactgc ttgaggacct actagcattt cgcaaaagat tttttcaatt
     3421 ttctttccta tctcaacttg atataaatct aaaccaaata tttccttatt ttctaagatt
     3481 tcctgtaccg cacaatgaat ctttgcagga tcttgttcac ctaatttcaa tactgagaat
     3541 ttttcctgca actctaccaa taaaggatca ggacttggca caaaattttt tccttgatca
     3601 tcgactgcca ataaataacg taaccaagca gcaatagcga taggaataaa tgttagattt
     3661 tctgcatttc cgtctttttc gacatacttt ttaattgttt caccatagcg aatcgctact
     3721 ttttgagaag tatctgttgc aattcgttgc ggtgtatctg gaatataggg attaggcaaa
     3781 cgtttattga ttacttcatc aataaattgt tttggatcaa taacgcctgg actttcaact
     3841 actggtaagc cttctacata acctatccct ttaattaagc caaccaaatc atcatcttgc
     3901 atttctgcct gaattgattt ataccccaac aaacaacctg ttattgctaa tgccgtatgg
     3961 agcggattta aacatgtcgt aaccttcatg atatctgctt tatcaactgt ttctctatct
     4021 gtcataatca caccagccgc ttccagttta ggtcgaccat ttggaaaact atcttcaatg
     4081 actagataat ggactttttc agtattggta aaagcagcga tattggtatt tttcgcagta
     4141 tgaattatat ctaagtcttc gaaacccgcc ttcaataaat ccattttgac actttccgaa
     4201 ggatttggcg taattctatc aatcatactc caaggaaaag tcactttttc tttatccttt
     4261 aaataagcaa taaatttttc agaaacgtat cctttttttt gccaagcttc tgcaatcata
     4321 aaaatgctat cttgaaaacg ttgaccattc tgtgagaaat tatcagtaga aaccatcgca
     4381 atcggtaact ctccagcatt aaacctgcta tacaacaagg atgtcacaat actcatcgta
     4441 tgttgaggat tggtaggtcc agattttata tctgcttcca ctaccgaaaa aaattgtcct
     4501 ttagaatctt ttaaactgta tcctttttca gtaatagtaa acgttgtaaa ctgtaatgta
     4561 ggatcttcaa aatatttaac cactctttga tagtccgtta tattctttgg attgcaatat
     4621 aaactatcag ctgttactgc taaaagtctt tttttgagtt caccatcttc acccataaca
     4681 atctccaaaa tatcattgtt gtagtcatga taagcttttt ctactacttc ttcatcaaat
     4741 gtctcacaca caactactcc ggattctaat tcattttgat ctgctaagca ttgagcaatt
     4801 tctgcatgaa atcctcgata aagatttcct ccaccaaaat gaagccacac tggatgtttc
     4861 tgaccttctt gtttgaccgc ttcgacatta aaatacggta gttgaacttg cattttgttc
     4921 aatccttcga tagaatcttg ccagtcttta atcaaaacca tatttacccc tccatactga
     4981 tatgttagtt tccgttacaa ttgtagtata aaatcgaatg taatcgctgt caataagaaa
     5041 aaactaaaaa tattcttttt tctaccatgt cagaattatt gtgtcttttt ttagtaaaag
     5101 cacaccaata tatatttttt agaactaact taaattaaag cttaatttct ctttgatctt
     5161 atttgagtat tttttgtttt tttaatagct agtatgttac tttgaaattt gatattatat
     5221 aacgagcata gaactaaaat atcaaattca aaaatgaaat tttgagaggg attaaatatg
     5281 acaattaaaa ctagtctcca aaatcaagct tatcaatcta ttcgtcagca aattatctac
     5341 gctgacttag aacctggaaa aaaagtatca gaaaaaaaat tggaagaaac gctttctatc
     5401 ggtcgtactc caatacgtga agcattgatt caactccgtc atcaaggact tgttgataca
     5461 attcctcaaa gtggaaatta tatctcatta atcgacttag atttagcaaa aaatgctcgt
     5521 tttgtcagag aacatttaga acgacaaatt atgctagagt gttgtgccaa gatgaataat
     5581 caaaagaaac aagttttaga aactataatt gcagaacaag aaaaagctgt tagtcaaaag
     5641 aatgctcgta gtttttttca taatgataat ttatttcata aagcttgttt tgaaattgca
     5701 gaacgggaag aagtatggca atggttggaa gataataata cgcacttgga acgatttcgt
     5761 tggttacgag taaaaacgga aggattaaaa tgggaaaaaa ttatggaaca acatcatcaa
     5821 ctatttaaag ccttgaccat gcaagaccct gaagaagcta acttcttaac atccttgcat
     5881 cttcatttaa tgcttgatga acaaaatgtt gtattgacta ctcacccaaa atattttaaa
     5941 aatatataac tgctaaattt ctttttttta atttatttac atttctgatt actacagaaa
     6001 actaactact ttttttatgt aaagttctgt caaatatagt cactttatat tactttctct
     6061 tgagtaaaaa aacatgtgag gtcgtaaaca aagattgaac tatctacatt catactctat
     6121 aatctgttta tttgcagact gataatagca aaagaaaact tataatcaaa taatatcaac
     6181 atttgatcac ataatttgag attgaatgct gatttaccta tgttttacgt aatggtcttt
     6241 agttattttt gctcataaaa tgcataaaac acgcgttttt atgtgtgctt gaaatttctt
     6301 ggctctataa tttctaagac tgataaaaat attctatcgg ttctttttgc aaataaaaaa
     6361 acatatagaa gctccattag attgaatcac cacaacacaa tcaaaggagc attccgtatg
     6421 cttgattttc attctataaa actactttta ggaatcaaag aaaatcacaa cggttcaaac
     6481 taaaagcaag aatatccgaa gtaccattgt ttcatcgatc atgagaaaag cattcacctc
     6541 tgtctgcaga tgttcttata tcgctctgtc agtcctatta ataaagtact gttttgtttt
     6601 caaaaaaact attacgagta ctttttaaga gaatgccttg caaatattca tcacacaccg
     6661 ttatataagt caaatagttt ctttcagtcg tcaagaaaag attctcctgt ccttggcatt
     6721 atcagttaag tagcaactta attctcggac ttgttctact ctagcaaaat ttattttcat
     6781 caacttacgt ccaaatgttt ttttgaataa ttacttcatc gcgggtctgg aagacttgcc
     6841 tcattcgata agtcactagc ttttggcgtc cttcaggatt tctagtatac accaactgac
     6901 atttgggcag cgacgattga tttaattcgt ttagaaaaag gttagcgatt tgcttatttc
     6961 ggccaaagcg ttctggtacc gtcagaacta tctttttccc ttcgattaaa taccgcggat
     7021 tctccagcgg tgctaaaagc tccctcatag cttcagtaaa aagctgtcct tctcgatagc
     7081 cagcattctc aagatagcat ctaaattcct gctgatcagc cgctacgatt acttttctat
     7141 cactaacttc accaaggcgc tgcaacgtcg tttgaacgca tatggccaag ctttttttgc
     7201 gggcacattc cttttcccat agcttttgtc gcctacgaat ttggaaatat tccagcaagt
     7261 tacctttaaa tatagctgac cagaagctta gaggctctat attttttact actatcggca
     7321 atgaagtttc ttgtttttgc tttacctgat cgactttcct aacgacgatt ggcatagata
     7381 ggtgccagcc atcagctaaa gcagtactcc attgttcttt caaccgactt cgttcacaag
     7441 aaaaatagaa catcgactga gtataagttt ccatcgtggt ggtcgtatat tccagtggta
     7501 aagcaaagcg atcgccacca ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa
     7561 tattgcccaa acgccgtagc attcttttca aatcaggctg ttcagacaga ggaactcccg
     7621 gtaatccaat cgctaaatgc caaatgttcg ttactttatt ttgtgcacct aggcgtaagc
     7681 cccgccctcg tagttgttgt gtttgcacaa aagtggaagc ttgattagcc aagatcaaca
     7741 gattaattgc aggacaatcc cagccttctc ctagcaaact aacggttcct accaaaatat
     7801 tcaacttctt ttcggttaat aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt
     7861 catccaccgt caaataaaaa taacccgaca tataaggatg ttcattcata cccgtcgcta
     7921 gttctgccag ctcaacagtt actagcattt tcggtaacag taagaactct ccacagatgg
     7981 caccgatttg ctgagtgtta cctaatagac gaacaacttc atcaaacaaa ggaaccacgc
     8041 cataagctaa ggtaccatca ctgcctgcta atacctcctt gcaaatacga tccatcaaaa
     8101 tgaccgctgt cagttcttgc ttcagctgaa cttcttcttt taacaaaatt tgacaaactg
     8161 cttgcttttt ctcaggaatc gcctgactac caacagtgtc tggatactct ggaaacaaat
     8221 gcagcgactc atcaaacaat catccagctt ttttcaattg ataaaaaata ggcaattccg
     8281 gttgatcttc aaacatctgc tgataaatag tgatcagctc ttttttacct aaagatggaa
     8341 ctgaagcttt tttaccttgc tgcttagaac tgcctaatag ctgccaatgt tcgggtatca
     8401 attcatagtt atgatgattc aagtaaatta atgcactagt gaacagctca aaattttcat
     8461 aaatttcctt cgaatgctca agcggatgct gaacaaattc taaagaaagc aaataatcca
     8521 ccgtctcttg gctttccatt aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa
     8581 aataatgctg agtagtttcc tgattagccg gaatcaaata tagaaaatcc tgatgaggac
     8641 atagaacctc ctcctttacc aactcgggaa tgctaatttc ttcgtcaatc aggccatttt
     8701 aactcaatat attgcttcca ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag
     8761 gaaatcgtca acagattcgg ttgttttttc ttcaagtcaa tcaacaattc accccagctt
     8821 ttttttaaat gatgagcttc atcaaaaaac taacagctcc agctgatcac cataagttgg
     8881 ctcctccttc agtcggttgt aaatggtctg atacgtttcc actgtaatat gactaggatg
     8941 ttcaatcgcc ccagaaagat agcaactatc tgctggattt actaagaaat attcctcgat
     9001 tgttgttaac cactgtttct tcaatattag ggaaggaacc aaaattaagg ttttacaatc
     9061 caaacgtcgg atcatctcaa ttcctaaaaa ggtcttgcca gcccctggtg gagccactag
     9121 atggactgaa ttgttattta aataagtagc aaagttagtc aatatacgct tctgatagct
     9181 acgccactga attttttcat cagatagcca catcccaggt ccccctctca cgtattttct
     9241 ctatcataat ctaaatgaat acagcaaaaa aaagatttct tcaagaatat gtgtttttat
     9301 agtaatattt tacttactct aaactcaaat gaaaaggtaa aaaaactggt agtacaatct
     9361 tttaaattct cattgttttg tacgttcttt actatcaaaa aaacaaaaaa aacgcgtttc
     9421 tacgcgtgac caaaattttt agctctataa tttctaagac tgatagaaaa tcattctgtt
     9481 tttgaagtct tttttcataa atactacttg atttattgtg tatatt
//
LOCUS       DACDSR010000095         9378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDSR010000095 DACDSR010000000
VERSION     DACDSR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.096"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..414)
                     /locus_tag="IWK15_002347"
     CDS             complement(<1..414)
                     /locus_tag="IWK15_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9547889.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLP"
     gene            complement(404..2578)
                     /locus_tag="IWK15_002348"
     CDS             complement(404..2578)
                     /locus_tag="IWK15_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9547890.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2594..4936)
                     /locus_tag="IWK15_002349"
     CDS             complement(2594..4936)
                     /locus_tag="IWK15_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547891.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4958..7501)
                     /locus_tag="IWK15_002350"
     CDS             complement(4958..7501)
                     /locus_tag="IWK15_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9547892.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7511..7909)
                     /locus_tag="IWK15_002351"
     CDS             complement(7511..7909)
                     /locus_tag="IWK15_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9547893.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7990..8274)
                     /locus_tag="IWK15_002352"
     CDS             complement(7990..8274)
                     /locus_tag="IWK15_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547894.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8298..9296)
                     /locus_tag="IWK15_002353"
     CDS             complement(8298..9296)
                     /locus_tag="IWK15_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9547895.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2670 a         2005 c         1468 g         3235 t
ORIGIN      
        1 aggaagaaat tgattcaatg tcttttcaaa aaagaatgtt tttctctcaa tttttttgac
       61 gtttctatca tgaatcacga gaaaacttgg gacactttta acatggtaac gttgtaaaaa
      121 agtttgaagt tcttcttgtc ttggttcttt tcttgtttca attcgatata cattcctaga
      181 gaattttttg tcgtatcttt ccaagaaaga ttcaacttct tggcattttt tacaatcatt
      241 tcttgctaga tacacaaaaa atgattgatt cgttttttga agttctagta cttcttctaa
      301 attactattg attagttttc catttgattc ttcgttagtt tttatcagtt taaaacaacc
      361 aaataagagg aaactaacaa taagtatcct aacaataact ttcttatttc gcatattgtt
      421 tctccaaatt tggtttccga atatagacga ctcgcggtgg ttcctggacg tcttcagctg
      481 ttcgatacac cacaaattcg cctgttctca atgctcgaat gcgattcgga ttgaatttaa
      541 agcggtctac gtctcgtttc gtgcctcttt ctcctgtcca atcaaccttt cccatatcag
      601 aataacctgc ttcttgtgtc gtcatttccg ttaattcaat gtcttcttct gtccccatta
      661 aatctgccca ataatcaatt tctgtcgggt catttgtctt cccaataaag aacgtattca
      721 cattcccaat caactgtttt cgaaggacag gatcaattct atcaatgtcc gcaagcgttt
      781 gaggggcata cgccgcatgc accccagcgc ctcgtccctt attgactaag tctataaggt
      841 tatcatttag ataagaagct ggttcgtcaa atattactag gactttcgag cgattggctg
      901 tttctcctgc ttgtttatac acttctgacg caaagaaatt aatatctccc acaagcattt
      961 gagccaatgt ccgaataaac tcactataaa tcaatccgtt taacgagata taaacaatct
     1021 ttccttcttt aacaacttct tgaatatcta attctctttt tcctccagaa gtatcaaaga
     1081 gttcaccaag ctcactatcc aataacatat tgatattggt ctgtaaattc tcagaagtcg
     1141 catacaaata aaacgggttc ttcttatgtt cgtaacgaac aaacaaccgt ttaaacaacg
     1201 tttgctcgat ttctgtcatg agagaaatgt tttcttttaa taatagataa tacgtttcta
     1261 agtcaacggt tttacgatca tgttcaacga ttcgctcttc acttaactgt tccacatcgg
     1321 caaaatcacg ttctgttttg tccatcgctt cattcttttt cgtttttttc ttcgtggttt
     1381 tcttcttttc tacgaccact ttttctttaa ataaatctgg atttttcaac tgaatacgat
     1441 ccgcaaacaa atgtaagacg tttcgtggca ataaataatg ttgtaaccaa actagatttc
     1501 tttcaatttt atggctcttt tggaactcgt ctaaaagctg aatcgttacc tgtaataacg
     1561 ctttggctgc tgtcgaataa tacacgcttt ctgtttctgc tagattggtt aatttatctc
     1621 gaatggcaat atcattgcca ttttttacag gattataacg caaatcgcca ccgtccacaa
     1681 agacttcaat cgggcgattg tatgcctctg ccacttcttt taacgcttgt aaagtctctc
     1741 ctgctccttt cccatcgact aagataacgg ggatacgaaa cttacacgca tgttctgcta
     1801 gtaattggat caccgtcgtt tttcctgaac ctgtagtcgc tggaataatc acatgttggt
     1861 tcaattcact aaagttcatc acataaggga gcccatactc atctgttcct aatagaatca
     1921 ctttggctaa tgaagccagt tgtttattcc gcaaagcaac gtcttttatg acaattgcta
     1981 cctggtacgc ttgctgatac tctgctgtat atttttggga ctcctgaaat ttattcttcc
     2041 gacgttcctt gtattcttta ctatttagca aagctgtttg ccgttctttc tttgaaacaa
     2101 cttttctttt ttcaataaaa gacgcatacg caatcaaaca acttcctgca cctgtcccac
     2161 ctaaaaggag ggcaaaaatc gtttgtccat taaactgcaa cgcatgagaa aacaagttta
     2221 atggaaataa taagtaacac gacaaatacc ctctaggaag aacaaaaata aaacaaaata
     2281 gatttaatag cgtaaaacct aacgcaaaaa taaaaaaaag agagcttctt cgttttaaga
     2341 agctctcata ctcaaagtca taaatatgtg cacgtgtctc ttttttatct atcataaaga
     2401 caatcgctaa aggaatactg cctacaataa aaaatggata tagacaaaca cctaataaag
     2461 tgacgataaa ataaagttgt tgaaaccgac tttttggcgt ttctttttct tgttttcttt
     2521 tgcgatcgac agaaaacatt tgctcaaaat gtgtcggtcg ttttggtgca ttcatcatac
     2581 tatcgatttc ctcctatcgt tttcgttgtt tcatttctct tcgataaatc ggttgttttt
     2641 ctcgttttcg tttcattttt gcttgagttg ctcgaaactc actccgtgtc ttcggtgctt
     2701 gttttggtga ttttgtttgt ttcatggttg agtatgcttt agatacggtt gaaggcgctt
     2761 tggtttgtgc tgttactcga ttgactgctg acatatgttc ccgaaccccc tctggacgtc
     2821 gaacagattt cggtgtggta gaacctgttg ctcgtggtac attccctttt cttagtggtt
     2881 tttgtaccgc atgtttttgt cctgcaactg tccgggtttt catcgaactt gtttttgtta
     2941 cgggagtaag tgcctccttc cgacgacgac ttgtttgtcc ctgacgacgt tttcgatcgt
     3001 tcaattctgt tctaggcgta tgcttctggc cataagactt gtgtttttgc tttggattcg
     3061 ctaatgacac caccattgca gaaccctgat tgcgatacgg actacgagac gcaccttttc
     3121 tacttctttc tcttagacta ggattgcctt gcaattctcg ttgtaataaa tcggctgttt
     3181 ttcttgcctg tttctccatt gctgttcgtt gcatacgttc ttgactttgc tcttctgctt
     3241 gtttcgcttg atcatgcaag tctcttgctt tttgtgccat acgttccata cgatttggtg
     3301 ctttatgacg aggttgttgt gcggattcac gccgaaaagc acttcgtgtg gcttgtttcg
     3361 ccgtttgtgg catacgaacg tctgagccac ccccacgttt tcgttgacgt tcttgtctct
     3421 tccgtgcgtt ttctttttct ttttgtttct gtgcttttct cgcttctttg cgttctttca
     3481 accgttgttt ggcttccctt ctcaattttg cttctgcccc aaatggattt cccgcaaaag
     3541 ctaacgctcc tcgaacaccc attcttccag taaatagact gcctaaaaag cctcggaaca
     3601 aataaattcc caacggcgca aacaaataaa caatgatcaa gagaattttt tcccatggat
     3661 cgtcttgtgt actacttaat tggaacccgt aactaagaaa agaagaaaaa aggatacatg
     3721 caaaacctgt cattcctttt aaaaacgtca aaataccaaa aactttggca tagttaccaa
     3781 acacattcat tgtgacaaga aacaatccta aaaaaagtaa gaacggcaac atcggtacta
     3841 acaatagttg catagttcca ataatcaatc gaaacatact taagacaaaa taaacaacgg
     3901 tttgaatcag attcacgaca aaataaaaca aggcaaatgc tgtatttttc caactattgt
     3961 aatattgtat gttcttgttt ttcagtgtat ctgcttcata ctctgtgact ttgtcatgaa
     4021 ttttctcccc tatttttgta cctaaatcat tgtccaataa aagattgatt cgatcatatt
     4081 cttttccagc atatttgact ttcttctcac gaattgtgtc tacattggtt gtgccataat
     4141 ttaacaacag atatggttca taaacattac tataaaagac ccgagccgcc atcaatttgc
     4201 ctgccgtttc cactttttta tccgctgtta cctcttctgt ttcttttgtt tcataatttg
     4261 tttcttcttc aggtgttaac actggattga ccttcacgaa cgccgtttcc actgtagaat
     4321 ctaaagaaaa aagaacatca aaaagattct tatttgtccc agaatctttt aatacgctta
     4381 acccagtaaa aataaggatc gtcataagaa agacacggag tgcttgtttc attcgagcct
     4441 ctccagcata tttgattgcc atcataattg ccgaaaaact gattccaata gttcctgcaa
     4501 tacttaacat attgtccgca attttttgtg ttaagttttg aatgggttct cgaatggcgg
     4561 aaataatatc taattcaaag agaaattcaa ccattgaagc attgaattct cctaacattt
     4621 ttacgccact ccaaacaaaa tttttcatca ctcctgcaac attgtacgat agcttttttt
     4681 taaggttcat tgactcatag tcattggtca tcaattcaaa cgaattcttt ttgtaatttt
     4741 tatacaattg atccacatca gtcgttactt cttttacttc tactttatcg ttggcattct
     4801 ctccttcaga acgatcggtt tcagtgacac ccgcaccatc attcatttga tcggcagaaa
     4861 cagtagcccc ttgtagacag aaaaaaagac cccctaataa aaggagcgtc caccatttca
     4921 gagatttttt tcgcaaaaaa accacctctt ttttgtttta tcgttgtttt tcattttcta
     4981 acattgcttg aattttttca tcttgatttt cagtagaaga aacagccaat aaccaactat
     5041 caaacaacac atcaatggca atcggttgat ttcttcccaa atgatcctgg aataaacagg
     5101 ttcctgtttg taattgttcc atcatttcta aattggctga attcacttcc atatcaaaga
     5161 aggacaatag tttctctcgt gcttcttttt gtttcggacg aaacgcaaat ttgtaagaaa
     5221 ttaactcttt tttatcttct tgatcgtaat ctttaaacgc ttgcacaaca aggtaaatat
     5281 ccgtattatt cgcacgtcct tttcgcaagc tatcatcaat caaatagctt ccttgtgcgg
     5341 tatcttcata cccttttgcc tcgtcataaa taatcgcagc gtcttcttct ttgtttgtag
     5401 aaaagatata cgtaaacttc ataatgactt ccataattgc cattcctgca agttgttcgc
     5461 tatttaatcg acccgaatca atctcttttt gtgtcggaat gcgtattcct tgtgtcccta
     5521 acacgttgat ttgtgaatca aaacgtatcg gcgtactgtg ctcattccca atgagaatct
     5581 ttcctaaacc aacgctatgc cctaaaatca atttagctag ttctttgtca ctttttcgaa
     5641 tttcttcgat aacttgactt aacgaatgct tacctggcat ggtgtaaact acatcgatgg
     5701 cgtcttggat caccgttttc ttgtggctcg ctgtttctcg attggtatct acttctccta
     5761 acattcctaa cacattttta gcggtttgaa ttgcctcttc ttttggtaaa aacaatagag
     5821 gatcaagcat ccctcgacag ctttcatctg aagataacga aataaaatta attcgttcat
     5881 acatagccac aaactctggt atgtgtccga atttttcaag tgcctttaaa aagaactttt
     5941 gggtttcatt tttcggatca atataaagaa ttttttgacc taaaaacatc agccataaaa
     6001 aaatatattt tacaaggacc gattttcctt tacctggtgg accaataatc aacgtatttc
     6061 cattggtatg ttccgcgcct tcaatacttt tctttgttaa attgggataa taccatacta
     6121 gtttactaga tagcgctaac gcttctttcg cagatttaaa cttttttgca ataacaatcc
     6181 gtcctaaagg aaatcctttc ttatttccta cttctttctt taagtccaag ccataatctg
     6241 ccacaaaacc tgtcgtagcg acctgctcgt aactacgaaa acgttgcgta cttccaatta
     6301 agcattgatt aaacaacgtt agctgatccg ctaccgatcg ataaaatttg aaatctgtag
     6361 actcaatcgc aaattcaata tctttgactc gttcttccaa ttcttctttt gaagaagcag
     6421 atacaaccag cgtgacggta gaataacaca atcgtttttc tttgcgcttc agcttatctt
     6481 ttacatccat taaccgttct gtaccaaaat ttaatacatc gtctgaatct aaaatgccgt
     6541 caacggtctc atattctttt tcttgttcat aaatccgtcc agccatttta ttaatttttc
     6601 gttcatcaga ttttgaatgc tcaaatcgaa gtcgaatatg cgtatcaatt ggaaaagata
     6661 agctatcctg aagattttga ataaagctac tcccatacat ggctaaagga gcgtccgttt
     6721 gacttaaaaa cgtcgaataa tgcgtttgat tcaactgttc aatcgcaatg tatcctgctt
     6781 cattcgaaat gacgccctcg caaatttcca ttggcgtaag ctgtctttca ggcaattttt
     6841 cttctgatcg atggaaaaaa taataataaa tttgtccaat ctcttgggca gataagcgct
     6901 ctatcggctt ataattggac aattccgtat agagctcctt ttctaatgtt tcatacgttg
     6961 gtaacacact acttatagga actcgttgtc ccgtaatacg atagataaac tttttaaaaa
     7021 catgcttaag caattgaaaa tattcgatcg gatcagacac tttcatttct gtagtgaaac
     7081 gcacaaacag atacgttcga tactcgttta attgcacttc gtcttttaaa atctttcctg
     7141 cctgttcaaa gtacactttc ccaagatctg cgcattctcc ctttaccaaa tctcgaatgg
     7201 tttcttcaat gtgcgtatct aggttaaatt gacgtggaat attgataaaa tgatagtcat
     7261 attcatcatg tccaagtatg ccttcgccat cttgaagata gttcttgaaa aaatcaatgt
     7321 catttaaagg aaatacttgg gaagaaagtt gatagcctac ccacacatca tcatttttgg
     7381 tcaaaacaaa atttcggata atggcgtcta tcggaaattc taatcgagga agcttcgttt
     7441 ttttcttttt gaactgccaa atcccgataa taaagacgac caaaagaaac aaaaacgcca
     7501 tttactcgac tcactccttc catttgtgta gggaatacgt cactttttca tggaaaaacg
     7561 gcacatacat ttcatgttca aagcgttgtt tccgtttctg ataataggac gttcgcccaa
     7621 ttaaatactc gaaaaagtgt ttattgtccc atttcattga aaacaatgtc caaacgagaa
     7681 caccaatccc aaaaatcata atcaaccagg actttcgaaa cagtgtctgc aagaaactaa
     7741 tttgattcac atacgcaatc aaaaatagga tcaaaagaat gaagagaaat accccaccta
     7801 tgactaattg ttccatgcga atgccattag aaatccaggg aattttttgt cctttaatcc
     7861 cgtaaatttt gtgagggtgc ttaaactcct ttgtatagtt gtattccatg ttcactctac
     7921 ctccttttac aaaaaaagaa gcgaccagaa gttctaatct tctgatcgct tttgcagttt
     7981 gtctttttct tatgataatt gtatccaggg cacaatcatt tgcgttaccc ccacgttctc
     8041 ttcactatcc ttaataaaga taatgttctt tagccattca ccaatatcaa cggcattcga
     8101 taacaataag acgagcatta tcaaaataaa gacgccaata atggcaaacg ctgcccgcgc
     8161 aaagccttgg gtcgcataag caccaaccat tagaacaatc gcaacgattg ccaatagcca
     8221 aatgacttgt ccagatacat catcaaataa cgatttaaga tcaaaaagtt tcatttccat
     8281 tctcctctaa gttcatttta ttcatttaac gtagtaacaa aataactatc gccacttgtt
     8341 tttacttcca ttgaaaagtt tgattggaac tcgtttcctt tctcaaaata gaaacgatac
     8401 ttcccttgca aaaagtagtt tccttcttta ttttttactg catgatccag ttgtacattg
     8461 atcaattcgc cgccttctaa ccctttttcg ttagaaatca atgccaattt ttcatcattt
     8521 ttgacatata actcaaaaaa ccgtttcaca aatgcttcca gcttgatttc ttcgctttca
     8581 gtgaccgctt gcccagaaga ataaaatcga ttttcctgat agttcacaga attggtattc
     8641 ttactctcag aaagattcaa aaattgaacg ccatcaagta cttttaaaac attgttttga
     8701 taactgactg gaaaagaaag ttgactgctt ttccaatgtt ctccttctgt aaaagtaacc
     8761 tcataaaata ggcgatacat tgcttgtgtc aaatcgattt cttctagttt aataaagcga
     8821 acatctttcg ctactcgatt ggtagccgtc gttacaaagc ctagatcaga cgcttccaat
     8881 ccttgtgcta aataatgatt gacggaagcc tcccattcgt ttcgtttttc tggcgaataa
     8941 gtaaataacc tttcaataaa atgttgtccc tcgtacttca attgttccgt agaagacaag
     9001 gtttggttga cttcagttaa caatgtttct ggattgactt gttttttcac tgctaaacgc
     9061 gttaatgtat ccactcgtcc aaagtttagg aggagcacga cacccattaa cagaaacaaa
     9121 caaaaaaaag cgcctgaaaa tacactcatg gcactttttt gcgaaaattg gatcattcga
     9181 tctgacggtt ctgttttctt tttttggaac aaaaaacgtt tcttttgttt cttttccttc
     9241 tgttgtttcg gtggcttagt ttggaacgct tgtggattaa attttgcatt actcatgaga
     9301 aatgccatac cgttctaaca ataatttcgg tattttgtcc acaatcacta cttcgtggac
     9361 aaccacactt gccccctc
//
LOCUS       DACDSR010000096         9352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDSR010000096 DACDSR010000000
VERSION     DACDSR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.097"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IWK15_002354"
     CDS             complement(93..761)
                     /locus_tag="IWK15_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9547896.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IWK15_002355"
     CDS             895..1182
                     /locus_tag="IWK15_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547897.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IWK15_002356"
     CDS             1598..3136
                     /locus_tag="IWK15_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9547898.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IWK15_002357"
     CDS             3133..4098
                     /locus_tag="IWK15_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9547899.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IWK15_002358"
     CDS             4085..5407
                     /locus_tag="IWK15_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9547900.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IWK15_002359"
     CDS             5648..6391
                     /locus_tag="IWK15_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9547901.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IWK15_002360"
     CDS             6406..7398
                     /locus_tag="IWK15_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9547902.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IWK15_002361"
     CDS             7410..8147
                     /locus_tag="IWK15_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9547903.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IWK15_002362"
     CDS             8137..8859
                     /locus_tag="IWK15_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9547904.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9352)
                     /locus_tag="IWK15_002363"
     CDS             complement(8943..>9352)
                     /locus_tag="IWK15_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9547905.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1605 c         1969 g         2711 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg tt
//
LOCUS       DACDSR010000097         9276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDSR010000097 DACDSR010000000
VERSION     DACDSR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.098"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..234
                     /locus_tag="IWK15_002364"
     CDS             34..234
                     /locus_tag="IWK15_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9547906.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            381..3170
                     /locus_tag="IWK15_002365"
     CDS             381..3170
                     /locus_tag="IWK15_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9547907.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3217..3744
                     /locus_tag="IWK15_002366"
     CDS             3217..3744
                     /locus_tag="IWK15_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9547908.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3765..4955
                     /locus_tag="IWK15_002367"
     CDS             3765..4955
                     /locus_tag="IWK15_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9547909.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4995..6293
                     /gene="asnS"
                     /locus_tag="IWK15_002368"
     CDS             4995..6293
                     /gene="asnS"
                     /locus_tag="IWK15_002368"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9547910.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7469..8143
                     /locus_tag="IWK15_002369"
     CDS             7469..8143
                     /locus_tag="IWK15_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9547911.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8140..8481
                     /locus_tag="IWK15_002370"
     CDS             8140..8481
                     /locus_tag="IWK15_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547912.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8511..8870
                     /locus_tag="IWK15_002371"
     CDS             8511..8870
                     /locus_tag="IWK15_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9547913.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9074..9253
                     /locus_tag="IWK15_002372"
                     /pseudo
     CDS             9074..9253
                     /locus_tag="IWK15_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         3103 a         1666 c         2013 g         2494 t
ORIGIN      
        1 aatatgtgtt gatacacaag gaggattttt attatggaaa caggtacagt aaaatggttt
       61 aactcagaaa aaggatttgg attcatcact gcagaaaacg gaaacgatgt attcgttcat
      121 ttctcagcta tccaaggcga cggattcaaa actttagaag aaggccaagc agtaactttc
      181 gatgttgaag aaggccaacg cggacctcaa gcaacaaacg ttactaaagc ttaattaacc
      241 tatactaaaa aataaacgcc cagacatttg cgtgtctggg cgtttttgtc tattttcttt
      301 aaaaagcgat gagagtattt ttctgcactt tgtagtataa tttcaaaaga acattagagg
      361 caggagaaga ggaagatatt ttgaaaccaa gaacttatgc agtcgttgat atcgaaacga
      421 ctgggacgaa tccaaaagaa gatcggatca tccaatttgg ctgcgtcatg attgaatcag
      481 gtcggatcac ttcacgtttt tcgatcgata ttcatccagg gcggaagatt tcaaaacaaa
      541 tccagcattt aacagggatc acgaatcaac gcgtccaaaa agcgccttat ttcgaagatg
      601 tcgcccaaac gatttacaat cttttggcag acaccatatt tgtcgcgcat aatatttatt
      661 ttgattataa ttttttgaac catgaactca tgcgatgcgg acttcctagt ttgaagatac
      721 caggcatcga tacagtagaa cttgcacaaa ttttcttgcc gacggaacca agctttcgct
      781 tagctgattt gtcagaaagc cttggattta ttcatgagaa tcctcatcag gcagatagtg
      841 atgccgaagt gacaggtcaa cttcttttgc tgatcgaaga acggatgaga aaattgccaa
      901 tcatcacgtt agagcagatt gcgcgattaa gtagacacac aggtatggat acgagtcgtt
      961 tcatttatca tgtgattgaa gaaatgaaag aaaaaccaga gccacttgat ccctcattag
     1021 aaatcgtcga tgggctagct ttgcagaaaa aagaagtgga actattcaca tccgtccatt
     1081 atggcgagcg gacatatcca agaaaaaaac aagcaaaaga aaaaatgttt ggtaaaacac
     1141 tgatgtatcg aaaagaacag aacagactga tgaatgcggt ctacgaccat ttcacaaaag
     1201 atgaatcaaa agatttgatg atcgaagcag ctacaggaat gggtaaaaca atcggttatc
     1261 tgctgccatt gagttatttg gctacacctg aaaaacccgc tgtcatcagt accgtttcgc
     1321 tagttttgca gcagcagatc atagaaaaag acattccatt gctgaatcaa ttgctagatc
     1381 agcccataca acctgtaatt atcaaaagct atcgccacta tattgatttg cagcgtttta
     1441 aaggcacact tgtggaaccg ccagaacaaa aacaatatgc tttgtatcaa atggcggtct
     1501 tagtttggct gacgcaaacg aagactggtg atttggatga attacattta acaaatttga
     1561 accattcatt ctttgcagat atcgcgcaca gaggtattgg atttttagca agaaatcagt
     1621 cattttacga acaagacttt gttcgtttct tgcaaaagaa aatcaggcaa agtaattttt
     1681 tgattgtcaa tcatgcgttt ttagtacaag aaacacagcg aaaagaacca ttgcttccag
     1741 ctagtccatt tttgttgatc gatgaagtac atcatttgcc ggaaatcgct gaaaaagtaa
     1801 atgacaaacg attaagtatt aactatttcc ataaacaaat ccagcaatta gaagaaccaa
     1861 atcagctgtt tgaccgaata caaagctatt taccgaaaga acatgaagcc ttacgctggt
     1921 tatcgattta tcgagaagaa ctttctgcgc tatgcgaagc acagatttgt atggcagaag
     1981 gattaatgag tttagtgagc ggcaagcaac ctaacggaca gtatccagaa gaagtcatga
     2041 tgacaaaaga gatgttggat cagctgccac tggaatctgg gacagccatt gatcacctgc
     2101 agctttttta tcaggagatc cagcagctac agtcacaaat gcgtctgact tttgagatta
     2161 tccaagaaag ctggacaaac cagatgcgga tcgatttagc aagtttatat agcctatttg
     2221 atcaattaga caagcaatac ttgctgatgg atcaatggct ggaagactgg cgagatcatt
     2281 tgatccattg gttcgttcct gggaacacag cgcagcagat gatttttcat ctccatgatt
     2341 ttcaagcggc tatgttgccg tcgactgttt ggtatcctcg atatgatcgt attctttata
     2401 ttggaggaac gctgaagatc ggccctaatc gacactatct ctctaagagg cttggcattg
     2461 aagcagcacc tcttaaagtg attgcctctc cttatgatta tgagaaacag gcaaggatct
     2521 atgtgccaaa agaggcacct gccgtccatc aagtcagcaa tcaagcatat atcgattatt
     2581 tagagacagc acttacaggt ctgatcgaac aagaagatcg gccaatactt gtgttattca
     2641 cttctcatga tgttttgcag aaggtctatc agaaaatgca tctgtctatg ctagaaaaag
     2701 gacgagaaat actagctcaa gggtttggtg gaagccggga gaagcttttg aaacgcttca
     2761 ttttgtcaga aaaaagcgta ttgtttggcg ctgacagctt ttgggaaggt gtagatttgc
     2821 ctggagatgc tttgcagatc gttgtagtca cacgtttacc cttcgataat ccgcttcgcc
     2881 cgatggtcaa agctaggaac gcttatttag aatcagaggg gatcaatcct ttttaccagg
     2941 aatccgtacc taaagcagct ttgaagttac gccaagcatt aggtcgcttg ataaggacgg
     3001 agacagatcg tggcgtattg cttattttgg atcgaagact agtcactgca aaatatggcg
     3061 cacggatcat ccatgcatta ccgaaaaaag taccgatcaa agagctagct atggaggaaa
     3121 tctatcagga tatccatgaa ttcttaaaga aaaatgaaga aggaagttag aggagctgtc
     3181 ttttttcata attaaaatct gctataatga aaaggaatga agataaagga gcgacgaccc
     3241 ttgaagaaaa aacagacaaa ggaaaaaacg attaatcgcg tgttacttgg cttgatcgta
     3301 tttttattag cggtcatcgt gatcagctcg attttttact tgcgttccag tcgtccggcg
     3361 gtgcaggcga agaaagaagc agtagaaatc gcaaagaaat atgcaaatct tgaggaaacc
     3421 gatcattttt attggtttac tcgaaagaag acatactttt cactgacagg tacaaatgat
     3481 aaaggaaaag aaatcgccgt tatcattcct aaatctggtg aaaaagtaaa agtattggat
     3541 caaacaaagg gactgactga aaacgaagca aaacagacaa ttgctgcggc ccatcctgaa
     3601 atccaaatcg aaaaagctgc tttagggatg tatgatgatc agcctgtgtg ggaagtagta
     3661 ggaaaagaaa caaatgggaa actgaattat tacctgattg gctttgaatc tggagatgaa
     3721 gtcaagacaa tcaagggaat ttaaaaggta aaggaatgaa tgggatggag ttatcaaaaa
     3781 gaacaaaacg attagaaccg tctgtcactt tggcagctgc ggcaaaggcc aaggcattaa
     3841 aagcaaaagg cagagatgtg ctgagtttga ctgtgggaga accggatttt gcgacaccag
     3901 aaaacatcca agaagcagca atcgaagcaa tccgcaatgg gaaagctagc tattatacac
     3961 caacagcagg aatcccagag cttcgacagg caatcgttga ttatctgaag aaaaactatg
     4021 gacttgtcta tgaaccttca caaacagtcg taacagacgg agcgaaattt gctttgtata
     4081 cccttttcca aacgatcctt gacccgcaag atgaagtcat cattcctgtg ccttactggg
     4141 tcagttacgg tgagcaagta aaactggcag aaggacttcc tgtctttgtg aaaggggaag
     4201 aatccaatag ctggaaagtt actgttgaac agctagaaca agcacgttca gaaaaaacaa
     4261 aagccgtaat catcaattct ccatcgaatc cgactggaat gatttatagc aagaatgaac
     4321 tgcaggcaat cggtgaatgg gcagtaaaaa atgatatttt gatagttgct gatgatattt
     4381 atggccgttt agtttataat ggcaatgagt ttactccaat tgcaacgatt tcagaagcaa
     4441 tcaaaaatca gaccatcatc atcaacggcg tgtctaaaac atatgcgatg acaggctggc
     4501 gcattggtta tgcggtggga aatcctgtga tcatcaatgg aatgatcgct gttgcttccc
     4561 aatcaactag caatccgaca gcagtcagtc aatatgcagc tgttgaagca ttgaaaggtg
     4621 agcaagatac agtagaagaa atgcggaacg cctttgaaga acgcctgaat acgttatatc
     4681 cgcttgtggc agaactgccg ggtgtttctc ttgaaaagcc gcaaggagct ttctatcttt
     4741 ttcctaatgt aaaagaaacg ctacgcatgt gtaagtatga aaatgtcaca caatgggtag
     4801 aagatctttt agaagaaaca ggggtagcgc ttgtgactgg cgaaggcttt ggggcaccag
     4861 aaaatgtccg catgagttat gccactgatc gcgctacgtt agaagaagcg gtacgcagaa
     4921 tcaagcaatt tattgagagt aaaagtcaaa attaatcctg aagaaattca aggaatataa
     4981 aaggagagtc atacgtggaa caaattcaaa tcatcgattc gaaaaaccat gtgggagaaa
     5041 cagtaacaat cggcgcttgg gtcgcaaata agcgatcaag cggaaaaatc gcatttttac
     5101 aattaagaga cggaacagcc tttttccaag gtgtagttgt aaaaagcgaa gtgtcggaag
     5161 aagttttcca aacagctaaa aatctacacc aagaaacatc cgtgttagta actggagaaa
     5221 tccgtgaaga cagtcgttca aaatttggtt atgaaatcgg aattacaaat gtggaagtgg
     5281 taagcgagag ccacgattat ccgatcacac caaaagaaca cggaacagac ttcttgatgg
     5341 atcatcgtca tttatggctg cgttcttcaa gacaacatgc gattatgcag atcagaaatg
     5401 aaatcattcg tgcaacgtat gaattcttta atcaacatca ttttgtcaaa attgatccgc
     5461 cgatcttgac tggttctgca ccagaaggca ctacggatct attcgaaaca aattactttg
     5521 atcaaaaagc ttatttatct caaagtggcc aattatacat ggaagctgct gctatggctt
     5581 tcggaaaagt cttttcattc ggtccgactt tccgtgctga aaaatcaaaa acacgacgtc
     5641 atttgatcga attctggatg atcgaaccag aaatggcttt cactcatcaa gaagaaagtt
     5701 tagaagtaca ggaacaatat gtagcattct tagtacaaaa tgttttagat cattgcgaat
     5761 atgcattaga tgtactagga cgagacaaag aagtcttaaa aacttataca aaattgccat
     5821 tccctcgtat tacttacgat gatgcaatcg aattactgaa gaaaaatgga tttgatgaca
     5881 tcgaatgggg cgaagacttt ggttcacctc atgaaacgtt cattgcgaat tcattcgaac
     5941 agcctgtctt tatcctaaac tatccaaaag ccatcaagcc attctacatg aaaccacatc
     6001 caacaagaga agacgtggta atttgtgcag atatgatcgc accagaaggt tacggtgaaa
     6061 ttattggcgg aagcgaacgg gcaacagact atgatttctt gctagaacaa atccgtaatc
     6121 acggtttgga tgaaaaagaa tattcatggt atcttgatct acgtaaatac ggttctgttc
     6181 cgcactctgg ttttggtcta ggccttgaac gaacagtcac ttggattgct ggtatcgacc
     6241 atgtacgtga agctattcca ttcccacgtt tgttgaaccg aatttatcca taagaggtga
     6301 tgaaaaagct gttctgcatc aagtaattag aacagccaag caaaaacagc tgttcatgtt
     6361 tttgaattgg cgggacttat taccgcagat gtaagctttt gaataccgtt tagtggagag
     6421 gttgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtggaaa tggctttcca
     6481 catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc gtttaaggaa
     6541 gaggttgtga aatcagtgcc ttgacctgag tattagagaa acaaaacgga aaaatagctt
     6601 ctcatatttt accgtttttt tagctaatat cgagggtcag ctgattgaac accgtttata
     6661 tcaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
     6721 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaacgtt
     6781 tatttaaaaa aaagaactgc gaactctcgc agttcttttt ttaaaatacg ggataaagga
     6841 agtttccagc agacaagaga agaaagcaaa agaaattgct atttgattca caaggttcta
     6901 taacgaaatc gcaatcaatc aaacatatac aaagaaggtt attcaaatgg aaatctgatt
     6961 tgatgatgct tctgctgttg aaataaagca gcatctagta cctgaaaaca aactaatagt
     7021 aacattcgaa gatggagtag gtccgtattt tcagcatgca atgatccata tgtaagttta
     7081 attcatcctt gaaatcatcg gaccagataa agttatgacc aatctatccc aagcaatcta
     7141 gaagagatat tagtaaaaga acatttactg gaagtcttag atgagcctat aaatatatgt
     7201 tctgatggcg tcatcagtgg tctgattgac ggaagcttag gtgtgatcga tttctctgga
     7261 ccaaacaacg aaggaatcaa ggggaatccg gcacgttaag aaaaaaagta taaaaaacta
     7321 gacacttgat gagcgttcat tcaattgatt gaagggacta ggctggtgtc attttttata
     7381 catatatttt ctctttcgtc ttgatagggc ttatgggata atataaggaa gaaaatcatc
     7441 gttttattca aaaaggagtt tttgacaaat gaaaaaacgt atattattta tcggtgacag
     7501 cattacagac gcaggaagag accgagcaga agcaactgat ctaggtaaag gttatccact
     7561 tttgattgcc ggaaaactaa tctcccaata tccaacgatg aaatttgaat gcatgaaccg
     7621 tgggatagga ggagacaaaa ttgcagatct gcaagtacgc tggcaagaag attgtttagc
     7681 tttacaacct gactatgtat cgattttgat cggaatcaat gatacctggc ataatactag
     7741 cgatcaggct atattcggaa ctgagaaatc tgcgcagcaa tttgaatccg tttatcgaaa
     7801 attactaaat gaacttcaaa cagcggaaat ctgcaacatc atcttgatgg agccgtttgt
     7861 acttcctata accgacgacc gtcatggctg gcgtaaagac ttagacccta aaatccaaat
     7921 tgttcgtaaa cttgctaagg aatatggcgc attgctggtt cctttagatg gcatattcaa
     7981 tgctttaggg attgcttatg gtttttcgaa atatacaaga gaagatgggg tccacccaac
     8041 gattggcgga catgagatca ttgcacaaag ctggatagat gcaattgccg cagtcgattt
     8101 ttttagtgga gataaaaatg agcgaaagaa aagtgagaaa tgatggaaat acggtgggca
     8161 gtaaaagaag atttacctca aatcaatcag ctgattactg atcatccaga atcattcgta
     8221 aaagcaaaaa cggcagctga cctaaatgat aagaataaac tattgctcgt tgctgtaaca
     8281 aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc aacagtcaga agaacgggtg
     8341 gatcagcgac catcaggaaa actaatccaa cacacattag taacaaaaga aaatacggaa
     8401 gaaatccaaa aaaaatttgc agaatacttg atgctggaag ctaaaaaaag aagaataaaa
     8461 tcaatttttg cccagtttta atcaaaaaca gtaatagaag gagaaataca atgagttgtt
     8521 taggaaatgt catctggttt atattcggcg gactaatcgg tggtcttagc tggttagcag
     8581 cggggatact ttggtgtatc acgatcatag gtatcccaat cggtatgcaa tgttttaaga
     8641 tcgcctccct ttcttttgcg ccttttggca aagaagtaat ttatcaccag tctggcatgt
     8701 cattgatcgt taatatcatc tggctgatcg tttcaggcct accaattgct ctcgcacatg
     8761 tcgtcagcgg catccttcta tgtatcacga tcatagggat accttttgcc aagcaatctt
     8821 tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga aattgtataa aaagagaata
     8881 ataaaggtta gaaaacgcat agagtcaggt gggatacatg tatttcacct gactctatgt
     8941 gttttcctgt ataagttctc ccatgtgact tttacgtctt ttatttatat gggattactg
     9001 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     9061 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     9121 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     9181 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca ggggcaatga tcgaagagaa
     9241 aaagaaagaa taagccaaaa catctgtatg gaagaa
//
LOCUS       DACDSR010000098         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDSR010000098 DACDSR010000000
VERSION     DACDSR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.099"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IWK15_002373"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IWK15_002373"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9547914.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWK15_002374"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWK15_002374"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9547915.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IWK15_002375"
     CDS             complement(3191..4432)
                     /locus_tag="IWK15_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9547916.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IWK15_002376"
     CDS             complement(4450..5373)
                     /locus_tag="IWK15_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9547917.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IWK15_002377"
     CDS             complement(5594..8086)
                     /locus_tag="IWK15_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9547918.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IWK15_002378"
     CDS             complement(8093..8560)
                     /locus_tag="IWK15_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9547919.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IWK15_002379"
     tRNA            complement(8696..8769)
                     /locus_tag="IWK15_002379"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IWK15_002380"
     tRNA            complement(8827..8900)
                     /locus_tag="IWK15_002380"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDSR010000099         8767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDSR010000099 DACDSR010000000
VERSION     DACDSR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.100"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..289
                     /locus_tag="IWK15_002381"
     CDS             <1..289
                     /locus_tag="IWK15_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547920.1"
                     /translation="VEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTR
                     SAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(386..820)
                     /locus_tag="IWK15_002382"
     CDS             complement(386..820)
                     /locus_tag="IWK15_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547921.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1052..1762)
                     /locus_tag="IWK15_002383"
     CDS             complement(1052..1762)
                     /locus_tag="IWK15_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9547922.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1752..2612)
                     /locus_tag="IWK15_002384"
     CDS             complement(1752..2612)
                     /locus_tag="IWK15_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9547923.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2617..3255)
                     /locus_tag="IWK15_002385"
     CDS             complement(2617..3255)
                     /locus_tag="IWK15_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9547924.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3270..3569)
                     /locus_tag="IWK15_002386"
     CDS             complement(3270..3569)
                     /locus_tag="IWK15_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9547925.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3612..5096)
                     /locus_tag="IWK15_002387"
     CDS             complement(3612..5096)
                     /locus_tag="IWK15_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9547926.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5116..5577)
                     /locus_tag="IWK15_002388"
     CDS             complement(5116..5577)
                     /locus_tag="IWK15_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9547927.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="IWK15_002389"
     CDS             complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="IWK15_002389"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9547928.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6880..7650
                     /locus_tag="IWK15_002390"
     CDS             6880..7650
                     /locus_tag="IWK15_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9547929.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7923..8750)
                     /locus_tag="IWK15_002391"
     CDS             complement(7923..8750)
                     /locus_tag="IWK15_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9547930.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2647 a         1851 c         1381 g         2888 t
ORIGIN      
        1 tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa
       61 agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc
      121 agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa
      181 ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa
      241 tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg
      301 aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat
      361 tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc
      421 tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt
      481 cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat
      541 aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat
      601 acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac
      661 gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa
      721 atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata
      781 attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata
      841 caattctccc tctaataata cagttctttg actatttaat ctatcacata taactctcct
      901 gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat
      961 aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat
     1021 cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat
     1081 gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga
     1141 tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg
     1201 ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga
     1261 cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa
     1321 cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat
     1381 aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca
     1441 tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca
     1501 aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc
     1561 ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg
     1621 gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt
     1681 tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg
     1741 caattttttc ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct
     1801 tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga
     1861 aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa
     1921 ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta
     1981 tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg agccggatta
     2041 ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga
     2101 atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt
     2161 tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg
     2221 ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt
     2281 tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc
     2341 gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa
     2401 caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt
     2461 tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata
     2521 aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt
     2581 aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc
     2641 atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt
     2701 gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc
     2761 agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc
     2821 accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat
     2881 accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg
     2941 caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac
     3001 tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc
     3061 acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc
     3121 tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc
     3181 aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa
     3241 attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca
     3301 gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact
     3361 ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg
     3421 tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt
     3481 acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat
     3541 ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat
     3601 cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa
     3661 aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca
     3721 actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct
     3781 gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg
     3841 aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagcggct
     3901 ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga
     3961 atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa
     4021 aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga accaaagcca
     4081 aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct
     4141 tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga
     4201 tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc
     4261 atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa
     4321 aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt
     4381 aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc
     4441 tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc
     4501 actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct
     4561 actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat cctacctaga
     4621 tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt
     4681 ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt
     4741 aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc
     4801 attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa
     4861 ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag
     4921 ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga
     4981 agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc
     5041 tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt
     5101 tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt
     5161 cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat
     5221 attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt
     5281 ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat
     5341 agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc
     5401 gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc
     5461 atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc
     5521 cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa
     5581 actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca
     5641 tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat
     5701 ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat
     5761 ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg
     5821 acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta
     5881 actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg
     5941 tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga
     6001 ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt
     6061 tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct
     6121 tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc
     6181 gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg
     6241 aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg
     6301 gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg
     6361 aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga
     6421 tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca
     6481 cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac
     6541 atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc
     6601 cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc
     6661 atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag
     6721 catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc
     6781 aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat
     6841 aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat
     6901 attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact
     6961 ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta
     7021 ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac
     7081 cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct
     7141 agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa
     7201 acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat
     7261 tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca
     7321 aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt
     7381 tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat
     7441 gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct
     7501 gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat
     7561 tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg
     7621 ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt
     7681 atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat
     7741 aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg cttcttttat
     7801 aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa
     7861 agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca
     7921 ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct
     7981 attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa
     8041 gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac
     8101 tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag
     8161 gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat
     8221 attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag
     8281 atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc
     8341 aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc
     8401 agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg
     8461 atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc
     8521 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
     8581 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
     8641 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
     8701 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
     8761 ttccctt
//
LOCUS       DACDSR010000100         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDSR010000100 DACDSR010000000
VERSION     DACDSR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.101"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWK15_002392"
     CDS             complement(<1..173)
                     /locus_tag="IWK15_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547931.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWK15_002393"
     CDS             complement(593..1714)
                     /locus_tag="IWK15_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9547932.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWK15_002394"
     CDS             2042..3451
                     /locus_tag="IWK15_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9547933.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWK15_002395"
     CDS             3448..4176
                     /locus_tag="IWK15_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9547934.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWK15_002396"
     CDS             complement(4304..5215)
                     /locus_tag="IWK15_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9547935.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWK15_002397"
     CDS             complement(5232..6239)
                     /locus_tag="IWK15_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9547936.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWK15_002398"
     CDS             complement(6236..8365)
                     /locus_tag="IWK15_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9547937.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWK15_002399"
     CDS             complement(8370..8477)
                     /locus_tag="IWK15_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547938.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDSR010000101         8392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDSR010000101 DACDSR010000000
VERSION     DACDSR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.102"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            72..2681
                     /gene="clpB"
                     /locus_tag="IWK15_002400"
     CDS             72..2681
                     /gene="clpB"
                     /locus_tag="IWK15_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9547939.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2832..3503)
                     /locus_tag="IWK15_002401"
     CDS             complement(2832..3503)
                     /locus_tag="IWK15_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547940.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3496..3990)
                     /locus_tag="IWK15_002402"
     CDS             complement(3496..3990)
                     /locus_tag="IWK15_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9547941.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4010..5230)
                     /locus_tag="IWK15_002403"
     CDS             complement(4010..5230)
                     /locus_tag="IWK15_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547942.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5377..7212
                     /gene="lepA"
                     /locus_tag="IWK15_002404"
     CDS             5377..7212
                     /gene="lepA"
                     /locus_tag="IWK15_002404"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9547943.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7306..8388)
                     /locus_tag="IWK15_002405"
     CDS             complement(7306..8388)
                     /locus_tag="IWK15_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9547944.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2693 a         1609 c         1690 g         2400 t
ORIGIN      
        1 agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt taattaggag
       61 ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg attgctgaag
      121 ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat gtatggaaaa
      181 tctttttaca acctaatcat tttggtcgaa atttctatac ggatgccgga ttggatgtcg
      241 aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt tctggtggaa
      301 acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat gaagcagacc
      361 aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt ttagcgttga
      421 tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta tctgaaaaag
      481 aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca caaaaccaag
      541 aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg
      601 gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt
      661 cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg aaaacggcaa
      721 tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata
      781 aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat
      841 ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct
      901 tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga agtatggatg
      961 ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc ggtgccacaa
     1021 ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt cgctttcaaa
     1081 aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt ggattaaaag
     1141 aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta gcagcagcta
     1201 ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc gatttgatcg
     1261 atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa ttagatcaag
     1321 ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg
     1381 atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt cgtgaagaag
     1441 caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct gtttcagcaa
     1501 aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat aactatgatt
     1561 tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa gaattgaagg
     1621 aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa
     1681 atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag
     1741 gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg attggtcaag
     1801 atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga ttgcaagacc
     1861 caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga aaaaccgaat
     1921 tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta cggatcgata
     1981 tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct ccaggctatg
     2041 taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct tatacaatcg
     2101 tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc
     2161 tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa aatacggttc
     2221 tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact gctgatggaa
     2281 cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac ttcaaaccag
     2341 aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta gacaacgtga
     2401 aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat caagaaatcc
     2461 tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat gaaccagcat
     2521 atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca ctagctaaag
     2581 aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta cttgatggac
     2641 aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt gagactataa
     2701 aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat aaacagtgtt
     2761 ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg cttctgtcac
     2821 aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa gtaacgctca
     2881 tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat cgtatttgtc
     2941 agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt gccatcagaa
     3001 gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc ccagaactcc
     3061 caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa ccaaggactg
     3121 gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc aagagctgtc
     3181 aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat actgatcaaa
     3241 tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa caaaacgatt
     3301 gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa aatcaatgca
     3361 atccctaata aagattccac gataaatgtg attccttgag aagtcgaata tttttgtgtg
     3421 ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa agctatttgc
     3481 acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt ttacttctga
     3541 aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac ctattgtata
     3601 taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga tttttttcgg
     3661 gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga tgcctttcgc
     3721 ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat ctagtaaagc
     3781 aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca ttcgactgtc
     3841 tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga tcgaaagctc
     3901 agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat cacttttagc
     3961 aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat caaaaataat
     4021 cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt gtttcttcat
     4081 ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc atcatcaagc
     4141 aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt aagacacgga
     4201 cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga gcgacaggat
     4261 cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt
     4321 cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg atctgactat
     4381 cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc cagtagatca
     4441 tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga tcaagataga
     4501 acatttcttt tacgtgaaat atttcttcag ctacccaata gaacagaaca cctcttggtt
     4561 catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg aaacgccaat
     4621 actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg gcaaatttgt
     4681 cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata ccacttactg
     4741 ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca gacatgattt
     4801 cccatccttt acgccgatag taggggatac tgtaaggata caaataagag atccattgct
     4861 tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat ccatggttag
     4921 catattctgg ataagttcca actccagtca caccacccat tttataacgt gcaccgtgaa
     4981 tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg tggaaccatc
     5041 cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct cgtttattct
     5101 caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa agcaattcat
     5161 tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct ctaaaatctt
     5221 tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa aattctacaa
     5281 aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt cgttggtata
     5341 atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa aatgaaagaa
     5401 cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg gaaatctaca
     5461 ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag
     5521 ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta
     5581 gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat tgacacccca
     5641 gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt
     5701 ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt ttatttagcg
     5761 ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc cgcagcagat
     5821 cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag tgaagctgta
     5881 ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat cgtggaatat
     5941 gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt tgactctatt
     6001 tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat ggtcaaaccc
     6061 ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga ggtcggggtc
     6121 ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca
     6181 gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt agcagacaat
     6241 cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta ttgtggtctt
     6301 tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa actgcaattg
     6361 aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt tgggttccgc
     6421 tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga acgggaattc
     6481 aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa aacggatggt
     6541 acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat tcaggatgta
     6601 gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg tgcagtaatg
     6661 gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga tgattaccga
     6721 gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt cgacaaattg
     6781 aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc
     6841 aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt aagctttatc
     6901 gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta
     6961 atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc
     7021 cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt
     7081 tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat gaaacaaatc
     7141 ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga cgaggacgaa
     7201 ccgaagaaat agcaagggtt tgtttgaaaa cagtgtaaaa aataaaccat gtttacatgg
     7261 atttgccacc ggtttgccac cggtggtttt tttcggtggc aaaaatcaag aattctttct
     7321 aagattttcc atgatatcga ccgtttcatt tctcatctta tctgttacat gcgagtaagt
     7381 atccatcgta attgaaattc tgctgtggcc caatcgttca gagatttctt tcatttttgc
     7441 gccattttcg agaagaagtg tagcatgagt atgtctgaga gaatggaagt taaaagagag
     7501 ggagagtgca ttcgatatcc tcctagtatt ccatttcact acacttggcg taactagctc
     7561 accgtcttcc ttcgtacata ctgcatttga atcaatgtag agctttccat acttcattcg
     7621 attttctaat tgttgtttct tatgtttttt cagaattgct agcaaggttt gtccaataaa
     7681 aatcgttcga ttggaactgc ttgtctttgg cgtaccatat acccatgcgc cgtcattctt
     7741 taccatttgt ttctctacag taattgttcc atttgaaaaa tcgacatcat cccacgtcag
     7801 accacaaact tcgccaacgc gcattcccgt ataaaatcca atattcaaag gaatatagaa
     7861 aggatgtcct tcaggagtaa tttctagcat atgatcaaag tcctcaagag aaatgatttt
     7921 tagatctttt ttagtcgttg gtcgtgcttc atattttggt atttttacat acagcatagg
     7981 attttgcttg attaatcccc aaggataaac cgccatattc agcgcattct taaggacaga
     8041 gtgagtaata gtcattgttt tcttcgagta acccttttta aattcagcat tgatgaaatt
     8101 ttgtaaaaga gcaggggaga gatccgtaag ttttttcttt cctaaataac cgtttatatg
     8161 atttttgatg gtaaatcggt agttttcata ggtattgtat tttagattta gtttaacgta
     8221 ttcctccatc caaaaatcaa ggtattgttt tactcgagta tccgtaccta aaaagtattg
     8281 tcctgtttcg tcaatatctg ataaaacttt tcgtaaagca gcttcggcct ctggtcgggt
     8341 gtctccgcca actttctcca ctttttttct tgagccatca tcattgatat ct
//
LOCUS       DACDSR010000102         8352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDSR010000102 DACDSR010000000
VERSION     DACDSR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.103"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            159..710
                     /gene="lepB"
                     /locus_tag="IWK15_002406"
     CDS             159..710
                     /gene="lepB"
                     /locus_tag="IWK15_002406"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9547945.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(793..1335)
                     /locus_tag="IWK15_002407"
     CDS             complement(793..1335)
                     /locus_tag="IWK15_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9547946.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1572..1970)
                     /locus_tag="IWK15_002408"
     CDS             complement(1572..1970)
                     /locus_tag="IWK15_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9547947.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2230..3090
                     /gene="ylqF"
                     /locus_tag="IWK15_002409"
     CDS             2230..3090
                     /gene="ylqF"
                     /locus_tag="IWK15_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9547948.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            3083..3850
                     /locus_tag="IWK15_002410"
     CDS             3083..3850
                     /locus_tag="IWK15_002410"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9547949.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3906..4763
                     /gene="dprA"
                     /locus_tag="IWK15_002411"
     CDS             3906..4763
                     /gene="dprA"
                     /locus_tag="IWK15_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9547950.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4885..6963
                     /gene="topA"
                     /locus_tag="IWK15_002412"
     CDS             4885..6963
                     /gene="topA"
                     /locus_tag="IWK15_002412"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9547951.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6932..8308
                     /gene="trmFO"
                     /locus_tag="IWK15_002413"
     CDS             6932..8308
                     /gene="trmFO"
                     /locus_tag="IWK15_002413"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9547952.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2915 a         1458 c         1747 g         2232 t
ORIGIN      
        1 catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa tagcctaatt
       61 gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt tcttataata
      121 aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa caacgctata
      181 ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt gcgttcatgt
      241 tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa aacgtgttga
      301 agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt gatattgttg
      361 tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt ccaggagaaa
      421 atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag gaaccttatc
      481 taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac tttacactac
      541 aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt ggagataatc
      601 gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat attctaggta
      661 aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga gaaacaataa
      721 agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag tttactacct
      781 tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag ttttgataaa
      841 tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga atctacctag
      901 aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat tgataaaaag
      961 gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt ttatcttttt
     1021 atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa gaaaagcagc
     1081 ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca tttcaagcaa
     1141 accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa atccgaatgt
     1201 gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa gggctaataa
     1261 aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa cgtcttttgc
     1321 aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga taccataaaa
     1381 tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac aattaaaaaa
     1441 agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata aaaaaaatag
     1501 aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa aacgtcagat
     1561 tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc tagatcttgg
     1621 acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg gaaagtacca
     1681 ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcgttttc aacactaaac
     1741 gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt tataaatttt
     1801 ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac cattgaacca
     1861 gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata aaaagcaacg
     1921 gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat ttgtttcgtc
     1981 tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa aaatttagat
     2041 tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat gtgatagaat
     2101 gaggactggt tcactttcac ttaactaata gatggaattt ctttataata aaaaagtgaa
     2161 tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat gaaaggaatg
     2221 gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg acgagaagtt
     2281 tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg attaccttta
     2341 tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct tgttttattg
     2401 aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata ttttcgtaaa
     2461 caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa aggtctagta
     2521 cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg ggggatcaag
     2581 cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc tacgttgatg
     2641 aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt aacaaaaggt
     2701 cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg gattttatgg
     2761 cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc gatcaaagac
     2821 cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc ccgatattat
     2881 tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa attggcaccg
     2941 gaattactga tggatattac agcaaaacgt gggtataaag aagactatga tcgtggcagc
     3001 gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact ggatcgttgc
     3061 gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag aacgattgaa
     3121 aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc cacgcaaagg
     3181 cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat taattaagga
     3241 aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc accaatggat
     3301 tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg cagctgcagt
     3361 tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat tatcagagaa
     3421 aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg ggatcgggat
     3481 cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat tagcgatgat
     3541 ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg cgatgacctt
     3601 ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg tgtcgattgc
     3661 tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat atgacgtgct
     3721 ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac acttagaagg
     3781 acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg taaaaaaata
     3841 tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg aaaggcggtt
     3901 ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa ggaatcagta
     3961 gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa gaaatcggat
     4021 ttgaagacat cattaagata ggtaaaataa ccaaacatcg ggagctgttt cgcaaaagtt
     4081 ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt atcacttgct
     4141 ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt gtactgtttt
     4201 ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt ggaagacaca
     4261 cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata gaagctggat
     4321 atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa gcagcaatct
     4381 ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc tatcctgctg
     4441 aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt gaatatcctc
     4501 ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc gcagctttgt
     4561 ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata acagcacagc
     4621 aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta acagaacatt
     4681 ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg ggtgaagata
     4741 tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg acaaacgttt
     4801 tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt aggaacacga
     4861 caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt agaatcacct
     4921 gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat ggccagtgtt
     4981 ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa caattatgaa
     5041 ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa agccgcagca
     5101 aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga agcgattgct
     5161 tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt ggtcttcaat
     5221 gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat cgatgtagat
     5281 ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta ttcgatcagt
     5341 cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca atctgtcgct
     5401 ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga agaatattgg
     5461 agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt ctggggaatc
     5521 gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac tgctagaatt
     5581 gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag aaaccctgcg
     5641 aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa ctttcgaaca
     5701 agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg taaacaagga
     5761 acagtcggct tgattacata catgcgtact gattctaccc ggattgccga tacagcaaaa
     5821 gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag acatgatcac
     5881 cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg tccaacgagt
     5941 gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt gaaactttat
     6001 acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt agatacaatg
     6061 aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa aatcaaattc
     6121 aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga gaaagaaaat
     6181 attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga accaaaacaa
     6241 cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc actagaagaa
     6301 aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca aagacgctac
     6361 tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat tgtcaattca
     6421 ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga aatggaagga
     6481 gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga tcgtttctat
     6541 aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat tcaaatcaaa
     6601 gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat aaaattaggg
     6661 aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac gaaaccaatc
     6721 gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat tgaacgaaaa
     6781 tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga attcacttct
     6841 tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt cgagaagaaa
     6901 gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga catccaaaaa
     6961 taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag ggcttgccgg
     7021 aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat atgaaatgcg
     7081 tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag tatgttccaa
     7141 ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag aaatgcgccg
     7201 attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg gtggtgcctt
     7261 agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag agcatccatt
     7321 agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg tagtagcaac
     7381 aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg gttcagatgg
     7441 tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg atatggataa
     7501 agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact gtccgatgac
     7561 agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg cagaacttaa
     7621 atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa tggcacagcg
     7681 aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag atcctaaaac
     7741 tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag cttctttata
     7801 caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg ttttccggat
     7861 gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc gaaacagctt
     7921 tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg aagatctgtt
     7981 ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga gcggactact
     8041 tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt ttccacaaga
     8101 aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac atttccaacc
     8161 gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag acaaaaaagc
     8221 aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa aaacagaatt
     8281 agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga gacggatagt
     8341 ctcgctcttt tt
//
LOCUS       DACDSR010000103         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDSR010000103 DACDSR010000000
VERSION     DACDSR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.104"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            190..615
                     /gene="lacA"
                     /locus_tag="IWK15_002414"
     CDS             190..615
                     /gene="lacA"
                     /locus_tag="IWK15_002414"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9547953.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            632..1147
                     /gene="lacB"
                     /locus_tag="IWK15_002415"
     CDS             632..1147
                     /gene="lacB"
                     /locus_tag="IWK15_002415"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9547954.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1158..2090
                     /gene="lacC"
                     /locus_tag="IWK15_002416"
     CDS             1158..2090
                     /gene="lacC"
                     /locus_tag="IWK15_002416"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9547955.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2093..3073
                     /gene="lacD"
                     /locus_tag="IWK15_002417"
     CDS             2093..3073
                     /gene="lacD"
                     /locus_tag="IWK15_002417"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9547956.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3101..3418
                     /locus_tag="IWK15_002418"
     CDS             3101..3418
                     /locus_tag="IWK15_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9547957.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3418..5124
                     /locus_tag="IWK15_002419"
     CDS             3418..5124
                     /locus_tag="IWK15_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9547958.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5266..6672
                     /gene="lacG"
                     /locus_tag="IWK15_002420"
     CDS             5266..6672
                     /gene="lacG"
                     /locus_tag="IWK15_002420"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9547959.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6695..6967
                     /locus_tag="IWK15_002421"
     CDS             6695..6967
                     /locus_tag="IWK15_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP9547960.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            6988..7887
                     /locus_tag="IWK15_002422"
     CDS             6988..7887
                     /locus_tag="IWK15_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9547961.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2599 a         1409 c         1698 g         2522 t
ORIGIN      
        1 aacggtgtta tctatttttt aaaaaaacaa ataaaaaaaa caaaaaataa acaaaaaaag
       61 tgttgctttt tgtttgaatg cttgatatta tataaacaaa gaaatgatga aaacgttttt
      121 tcgaacattt tgcaaaatgt tttctacttc tacgtagcat ttctttctaa aatttaggag
      181 gtggtcaaaa tggctattgt tgttggtgca gatctcaaag gtacgagatt aaaagatgtt
      241 gtgaaaaatt ttcttgtaga agaaggtttt gaggtaattg atgtgactaa ggacggacaa
      301 gattttgttg atgttacctt ggcagttgcc tctgaagtaa ataaagatga gcaaaactta
      361 ggtattgtaa ttgatgctta tggagctggt ccattcatgg tagcaactaa aattaaaggt
      421 atggttgcag cagaagtttc agatgaacgc tcagcttata tgacacgtgg acataacaat
      481 tcacgaatga ttacggttgg cgctgaaatc gtcggtgatg agcttgctaa aaacatcgct
      541 aaaggtttcg tcaatggtaa atatgatggc ggacgtcatc aaatccgagt agatatgctt
      601 aacaaaatgt gttaagaaaa ggagttaagg aatgagaatc gcaattggat gtgaccacat
      661 tgtcacagat gaaaaaatgg cagtatcaga attcttgaaa tcaaaaggac atgaggtcct
      721 tgatttcggg acatatgacc acgcacgtac tcactaccct atctatggta aaaaagtcgg
      781 tgaagcagta gtaagtggtc aagcagactt gggagtatgt atctgtggta caggtgtcgg
      841 cattaacaat gcagtcaata aagttccagg tgttcgctca gcgttggttc gtgatatgac
      901 atcagctata tatgcaaaag aagagttaaa tgcgaatgtt atcggtttcg gtggaaagat
      961 tactggtggt cttcttatga atgacatcat tgaagctttc attgaagctg agtacaaacc
     1021 aacagaagaa aataaaaaat tgattgcaaa aatcgaacac gttgaaacac ataatgcaca
     1081 tcaagcagat gaagatttct ttacagaatt ccttgaaaga tgggaccgtg gtgactacca
     1141 cgattaaggt ataacaaatg attctgacag tcacactaaa tccttcagta gacatctctt
     1201 atcctctaga aacattgaaa attgatacag tgaatagggt gaaagatgtt agtaagacag
     1261 ctggtggaaa aggcttaaat gttactagag tcttgtatga atctggtgat aaagttactg
     1321 ctacaggttt cttaggtggt aaaattggtg aatttattga aagcgaatta gaacaatctc
     1381 ctgttagtcc agctttttat aaaatctcag gaaacactag aaactgtatt gctatcttac
     1441 acgagggtaa ccaaacagaa attttggaac aaggaccaac aatttctcat gaagaagcag
     1501 aaggctttct tgaccactat agcaatctta ttaaacaatc agaagttgtg acaatttcag
     1561 gaagtttacc ttcaggactt cccaacgatt attatgaaaa acttatccaa cttgcttcgg
     1621 atgaaggagt agcagtcgtt ttggattgtt caggtgcacc tcttgaaaca gttctaaaat
     1681 cgtcggctaa acctactgcc atcaaaccta ataatgagga actttcacag ttgcttggaa
     1741 aagaagtaac aaaagatatt gaagaactca aagacgttct taaagagtca ctttttagtg
     1801 gtatcgaatg gatagtggtc tcattaggtc gaaacggtgc ctttgcaaaa catggtgatg
     1861 ttttctataa ggtagatatt cctgatatcc cagttgttaa tccagtcgga tcaggggact
     1921 caacggtagc tggtattgca tcagctttaa atagtaaaaa aagtgacgct gacttattaa
     1981 aacatgcgat gacattgggt atgttaaatg ctcaagaaac aatgacaggg catgtgaata
     2041 tgactaatta cgaaacacta aactcacaaa ttggagtaaa agaggtataa aaatggtact
     2101 tacagaacag aaacgcaaat ctttggaaaa aatttcagat aaaaacggtg ttatctcagc
     2161 tttggcattt gaccaacgtg gtgctttgaa acgtttgatg gcacagtatc aagatacaga
     2221 accaactgta gctcaaatgg aagaactaaa ggttttggtt gctgatgaac ttacaaaata
     2281 cgcttcttca atgttgcttg accctgaata tggacttcca gcaacaaaag cattggataa
     2341 agaagcaggt cttcttcttg cctatgaaaa aacaggttat gatacatcaa gtacaaaacg
     2401 tttgcctgac tgcttggatg tttggtctgc aaaacgtatc aaagagcaag gcgctgatgc
     2461 cgttaagttc ttgctttact atgatgtaga cagctcagat gaacttaacc aacaaaaaca
     2521 agcttatatt gaacgtgttg gttctgaatg tgtcgctgaa gatattcctt tcttcttgga
     2581 aatccttgct tatgatgaag aaatctcaga tgcaggttct gttgaatatg caaaagtgaa
     2641 acctcgcaaa gtcattgaag cgatgaaagt tttctctgat ccacgcttta acattgatgt
     2701 tcttaaagta gaagtaccag ttaacgttaa atatgtcgaa ggttttgctg atggtgaagt
     2761 agtttacagt aaagctgaag ctgctgactt ctttaaagca caagaagaag cgactaacct
     2821 tccatacatc tacctaagtg caggtgtatc tgctaaattg ttccaagaaa cattgcaatt
     2881 tgctcacgac tcaggtgcca agtttaatgg tgtgctttgt ggacgtgcta cttgggcagg
     2941 atctgtagaa ccttacatca aagaaggtga aaaagcagca cgtgaatggt tgcgtactac
     3001 tggattcgaa aatattgatg aacttaacaa agttcttgtt aaaacagcta gtccatggac
     3061 tgataaagta tagtaaaaac ataaaacgga ggatattgtt atgaacagag aagagatgac
     3121 tctcttaggg tttgaaattg ttgcttatgc tggagatgct cgctctaaac ttttagaagc
     3181 gcttaaagcg gctgaaaatg gtgatttcgc taaggcagat agtcttgtag tagaagcagg
     3241 aagctgtatt gcagaggctc acaattctca gacagctatg ttggctcgag aagcttctgg
     3301 ggaggaactt ccatacagcg ttaccatgat gcatggtcag gaccacttaa tgactactgt
     3361 cttattaaaa gatgtgattc atcacctcat cgaactttat aaaagaggag caaaataatg
     3421 cataaactca ttgaacttat tgagaaaggg aaaccgttct ttgagaaaat ctctcgaaac
     3481 atctatcttc gtgctattcg tgatggattt atcgctggta tgccagtcat ccttttctca
     3541 tctatcttta tccttattgc ctatgtccca aatgcttggg gattccactg gtcaaaagac
     3601 attgagacct tcttgatgac tccttatagc tattcgatgg gtatcctagc attctttgtt
     3661 ggtggtacta ccgctaaagc tttgacggac tcaatgaacc gtgacctacc tgcgaccaat
     3721 cagattaact tcttatctac gatgctagcg tctatggtag ggttcctttt gatggcagct
     3781 gagcctgcaa aagaaggagg cttcttgaca gccttcacgg gaacaaaagg tcttttgaca
     3841 gcatttattg cagcctttgt gacagttaat gtttacaagg tctgtgtgaa aaataatgtt
     3901 actattcgta tgcctgaaga ggttccacca aatatctctc aagtatttaa agacttgatt
     3961 ccattcactg tagcggttgt tctcctttat ggatttgaac tcattgttaa gggaactctt
     4021 ggtgtaaccg ttgcagaatc aatcggtacc cttcttgctc ctctcttctc agctgcagat
     4081 ggttaccttg gaattacact tatctttggt gcttatgcct tcttctggtt tgttggtatc
     4141 cacggtcctt caattgtcga accagcaatc gctgctatca cttatgccaa tatcgatgcc
     4201 aacttgcatc ttatccaagc tggacaacat gcagacaaag ttatcacttc tggtactcaa
     4261 atgtttattg ttaccatggg tggaacagga gctacattga ttgttccatt cttgttcatg
     4321 tggatttgta aatcagaacg taaccgtgcc atcggacgtg cctcagttgt tccaacattc
     4381 tttggggtta atgagccaat cttatttggt gcaccaatcg tattgaatcc gattttcttt
     4441 gtaccgttca ttttcgctcc gattgtcaac gtttggatct ttaaattctt tgttgacaca
     4501 ttgaacatga actcattctc tgccaacctt ccatgggtta ctcctggtcc gttgggaatt
     4561 gtactcggta ctaacttcca agttctatca tttatcttag ccggactctt ggtagttgtt
     4621 gatactatca tttattaccc atttgttaag gtatacgatg aacaaattct tgaagaagaa
     4681 cgttctggta aaacaaacga tgctcttaaa gaaaaagtag ctgcaaactt caatactgct
     4741 aaagcagatg ccgttcttgg aaaagcaggc gttgctaaag aagatgtagc agcaaacaat
     4801 aatatcacaa aagaaactaa tgtccttgtt ctttgtgcag gtggaggtac aagtggcttg
     4861 cttgccaatg ccttgaacaa agcagcagca gaatacaatg ttccagtcaa agcggcagcg
     4921 ggtagttacg gtgctcaccg tgaaatgttg ccagagtttg acttggttat cttggctcca
     4981 caggtcgctt caaacttcga tgatatgaag gctgaaacgg ataaattggg cattaaactt
     5041 gcaaaaactg aaggagctca atatatcaaa ttgacacgtg atggacaagg tgctcttgca
     5101 tttgttcaac aacagtttga ttaataaatt tgtaaaagtt atttgagacg agattattct
     5161 ttcaattcct tataatattg ttaaccatat cattgcattt catttccttg gtttccggaa
     5221 ctcagaatat cgtctcaaat gctttttttg aaaggattta tagttatgac taaaacactt
     5281 cctaaagatt ttatttttgg tggtgctaca gctgcttatc aagcagaagg tgcgactcac
     5341 acggatggta aaggcccagt tgcatgggat aagtatcttg aagacaatta ttggtacaca
     5401 gcagagcctg caagtgattt ttaccataaa tatcctgttg atttagaatt agctgaaaaa
     5461 tatggcgtta atggtattcg tatttcaatt gcctggtctc gtattttccc aactggctat
     5521 ggcgaagtaa atgaaaaagg tgttgaattc taccataatc tttttgccga gtgccacaaa
     5581 cgtcatgttg agccattcgt gactttgcac cactttgaca caccagaagt tcttcactca
     5641 gatggagatt tcttaaaccg cgaaaatata gaacactttg tagattatgc cgctttctgt
     5701 tttgaagagt tcccagaagt aaactactgg acaactttca atgaaattgg cccaattgga
     5761 gatggtcaat acttggttgg taaattccct ccaggcattc aatatgatct tgccaaagtc
     5821 ttccaatcac accacaatat gatggtttct catgcacgtg ctgtgaaatt gtataaagat
     5881 aaaggttata aaggcgaaat tggtgtagta catgcgttgc caaccaaata cccttatgat
     5941 ccagaaaatc cagcagatgt tcgtgccgct gagttagaag atatcatcca taacaaattt
     6001 atcttggatg caacctacct tggacactat tcagataaaa cgatggaagg ggtcaaccac
     6061 atcctagctg aaaatggtgg agaacttgat cttcgtgatg aagacttcca agcacttgaa
     6121 gcagctaaag atttgaacga tttccttggt atcaactact acatgagtga ttggatgcaa
     6181 gcctttgatg gtgagactga aatcattcac aatggtaagg gtgaaaaagg aagttctaag
     6241 taccagatta aaggtgttgg tcgccgaatc gcaccagatt atgtgcctag aacggattgg
     6301 gactggatta tttatcctga aggcttgtat gaccaaatca tgcgagtgaa aaatgattat
     6361 ccaaactaca agaagattta catcactgaa aacggtcttg gatacaaaga tgagtttgta
     6421 gataataccg tttatgatga tggtcgtatt gattacgtga agaaacactt ggaagtttta
     6481 tcagacgcaa ttgcggatgg tgcaaatgtt aaaggttact tcatctggtc acttatggat
     6541 gtcttctcat ggtcaaacgg atacgaaaaa cgttatggac tattctatgt agactttgac
     6601 acgcaagaac gctatcctaa gaaatcagct cattggtata agaaagtagc agaaactcaa
     6661 gtgatagagt aatattactt gaaaatgtaa aaaattgtta tccgatactt tagaaaaagt
     6721 acaacactta tttagaccag gctttgaaaa ggtatatatt gttagtttcg aagattgtcc
     6781 tatgattcct gagcttgaag ctaccccgtt gctaaaatgt ggtaaatggt atgtttcaac
     6841 tggaaaagag tggatttgcc attctgattt agagttatca gcatttgaat gggaattttt
     6901 acaatcactc gatgtagaaa tacgtgagac tattcacttc gaggtaaatt atttgccttt
     6961 ccaataataa aaagaaagga attagttgtg actatcgaat taaaaaacga gtatcttact
     7021 gttcagttta aaacacttgg tggtcaactg acttcgatta aagacaagga tgggatagag
     7081 tatctctggc aagctgatcc aaattattgg aatggacagg cgcctattct atttcctatc
     7141 tgtggtagtt tacgaaatga ttgggctatt tatagacctc aagaaagacc cttttttaca
     7201 ggtcttattc gtagacatgg ttttgttcga aaagaagaat ttattctgga agaagtcaat
     7261 gataatagcg tgacctttag tattaaacca aatgctgaga tgcttgataa ttacctatat
     7321 cagtttgaac tgagagttgt ttataccttg aatggtaaat caattagaac cgaatttcag
     7381 gtaacgaact tggaaatgga aaaaacaatg ccttacttta ttggagccca tccagcattc
     7441 aatagtcctt tagtagaagg tgaaaactat gaggattatt atattgaatt tagtgaggtt
     7501 gagtcttgct cgataccaaa gagttttcct gagacaggat tattagattt gcaagatcgg
     7561 acaccttttt tggaaaatca aaaaattctc gatttggact actcactttt ttcccatgat
     7621 gccattacac ttgaccagct caagtctcgt agcgtaacgc ttcggtcaag gaagtctggt
     7681 aaaggtcttc gagtagattt tgatgatttt ccgaacttga ttctgtggtc tacaactaat
     7741 aaaagtccat tcattgcttt ggaaccttgg agtggtctct ccacttcact tgaagaaggt
     7801 aatttcttag aagataaaag acaggtaacg aaaatttcac ctcaagaaac ttctagaaaa
     7861 atctatgata ttactatcct taattaaaat ttgttattat ttgagtcttc aataagattt
     7921 gaatactctt accttgtgat gatagattgg tcttctccgt gccagtctta catcgcaagg
     7981 tttttaagtt ttttgtgggt ctatgtcaac tgtagtgggt gacgaaaagc taatatctaa
     8041 agacagtctg gtagtaaaaa ataagattat ttactacttc ttgatttaaa ataatagtgg
     8101 agccattaat ttgtggcaaa tttaatttaa tattttgcct caaattagcc acacttttta
     8161 tagaactaag aggaaaaatg aaattttctt cctattaata tagttgtttg aaatcttaaa
     8221 tatagcgt
//
LOCUS       DACDSR010000104         7916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDSR010000104 DACDSR010000000
VERSION     DACDSR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.105"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..199)
                     /locus_tag="IWK15_002423"
     CDS             complement(<1..199)
                     /locus_tag="IWK15_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547962.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="IWK15_002424"
     CDS             complement(362..1996)
                     /locus_tag="IWK15_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9547963.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="IWK15_002425"
     CDS             complement(2000..3373)
                     /locus_tag="IWK15_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9547964.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="IWK15_002426"
     CDS             complement(3389..3628)
                     /locus_tag="IWK15_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547965.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="IWK15_002427"
     CDS             complement(3810..6485)
                     /locus_tag="IWK15_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9547966.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="IWK15_002428"
     CDS             complement(6668..7591)
                     /locus_tag="IWK15_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547967.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2321 a         1560 c         1198 g         2837 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgat
//
LOCUS       DACDSR010000105         7870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDSR010000105 DACDSR010000000
VERSION     DACDSR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.106"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..427
                     /locus_tag="IWK15_002429"
     CDS             <1..427
                     /locus_tag="IWK15_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547968.1"
                     /translation="RYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFME
                     RSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTY
                     LYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(528..1229)
                     /gene="nth"
                     /locus_tag="IWK15_002430"
     CDS             complement(528..1229)
                     /gene="nth"
                     /locus_tag="IWK15_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9547969.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKRNHACRRGFFNCK"
     gene            complement(1236..1934)
                     /locus_tag="IWK15_002431"
     CDS             complement(1236..1934)
                     /locus_tag="IWK15_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9547970.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2202..3386
                     /locus_tag="IWK15_002432"
     CDS             2202..3386
                     /locus_tag="IWK15_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9547971.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3392..3835)
                     /locus_tag="IWK15_002433"
     CDS             complement(3392..3835)
                     /locus_tag="IWK15_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9547972.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4061..4348)
                     /locus_tag="IWK15_002434"
     CDS             complement(4061..4348)
                     /locus_tag="IWK15_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547973.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4519..5871)
                     /locus_tag="IWK15_002435"
     CDS             complement(4519..5871)
                     /locus_tag="IWK15_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9547974.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6144..6590)
                     /locus_tag="IWK15_002436"
     CDS             complement(6144..6590)
                     /locus_tag="IWK15_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9547975.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6826..7752)
                     /gene="cysK"
                     /locus_tag="IWK15_002437"
     CDS             complement(6826..7752)
                     /gene="cysK"
                     /locus_tag="IWK15_002437"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9547976.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2136 a         1594 c         1277 g         2863 t
ORIGIN      
        1 tcgttattcc aagaataaaa aagctgaaaa attttgttta ttattaaata aatttacttt
       61 aattgctcct atccttgcat ttattatttt ttctgtactt ttaagcacta ctcttaaagg
      121 aaaatttatg gaaagaagct ctcatgcaat gattctcact tttttatggt tgttgttcac
      181 cagaatctat atttttctga tgagcctgaa accgcccaaa tccattagtt tatgtttagt
      241 tataaatggt atttttttat tatcacttat catttttatt acacctttag atagatacgt
      301 cacttattta tataatcctt tggaatactg gacttatttt ataggtatat tggaaggcat
      361 tattttctac atcgggtatt ttcccaataa aaataacaac ttttattttt atcgcaataa
      421 attgtaataa gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa
      481 gataaacgaa acagcttttc tccataataa caagataatt catacattta tttgcaatta
      541 aagaaaccac gtctacaggc gtggtttctt tttagcgcct tttcttttag acgcatccga
      601 tttttcccat cttgacatat tgatagcaaa ggacatacct cgcacttggg atttcttgcc
      661 gtgcaatgat atctaccaaa aaaaatcagt gtatgatgtg tctttaccca taattcttgt
      721 ggtactttcc gcatcagtgt ttcttctact tccatgaccg tagcatctag tttgcagatc
      781 ctcaggcgtt ttgagacacg ttccacgtgg gtatctactg cgattgctgg tatcccgaat
      841 gcatccccca atacgacatt tgctgtcttt ctgcctacac caggaagaga catcaattct
      901 tctcttgacg ttggcacttg cccatcgaaa cgttcaatca gctgctgtgc acacgctttg
      961 atgttttttg ctttatttcg atacaaacca atcgttttta ttttaagaat gatttcatcg
     1021 attgaagctt ctgctaacgc atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct
     1081 ttattcactg atacatctgt cgcctgggca cttaagatca ctgcaatcaa taactcaaat
     1141 ggattattgt gcttgagttc tccatgtgct tcgggaaaca ttccatacat cgtttctaga
     1201 gcttccattg ttttttgttt gcttaacata ttccgctact cctccaacca atttttcagt
     1261 gagactttcg gcaattcttc ttgttgagct gactgttcta cttctctttg aagaagctgc
     1321 tgttttcttc gtttttggtc ttcttccact tgttgttttg tacgtagatt ttttctttcc
     1381 cacgatagca aaatgcggtc aacgtaatta aaactataag cctgatttaa tacggcttcc
     1441 cgcagtgcta gcttcaaaat ttccggctga taatggtctt cttctagcca ttgtccgatc
     1501 cgctggaact cgatcgaaga aagcgggcgt ccaaattctt gttcaaataa ttgatacact
     1561 gactggattt ctttttcttg tgttttttct tcgtattttt tttcttgtgt ttttaaatac
     1621 tcacctaata gatcatagat ggggtaaaga ttatatcgat ctgctttttt accgttgaca
     1681 atcgtcgttt ctatcttgat aaatccattt gttacgagac gatccagtat ttgaaaaatc
     1741 tgatcttgtt tgattcccat atccatagca atcatctgca aatcagggaa agtatctcct
     1801 tcaagctgtg ccatatgcaa ctgtagaaca aataaaaatt cttcagattg tagaccgatc
     1861 ctatgataat ttttaagtaa aagattagat atgactgttt gtccagcgct taagtaattt
     1921 tctaaattca acatgatttc tcctccgctc aatcacagta tacgtaaaaa aaacatggga
     1981 agcaagaaca ggtttctttt ctatttactt cactgatcgg tcagatttac aatgtcgtct
     2041 acttgatttt tgtttgttca tgtttttgat gcgtatcttc acttttcgga ataaataaat
     2101 taagtagatt tcgctgttcc ttagaatgtt gacaaggaaa caaaattact atacactatt
     2161 tttgtttcca agttaacatt attgcataaa ggagttttta catgtacagt ttcacaaata
     2221 aacattctaa agtttttgga tacgtctctg tcttattcac tggattagga tttactatcg
     2281 tcagtcctgt acttccgttt ttaacgctgc cttattctca ctccatacat cagcaagctt
     2341 tctatatcac tttgctcatg tctgtttatg ctctagctgc cttcttatct gcccccattc
     2401 tcggtagctt aagtgatcat tttgggcgtc atccgatcct tattatcagt ttattaggat
     2461 caagtatcgg atatttgctt tttggtttag gaaattcgat atggatgttg ttccttggtc
     2521 gtatcattga aggattgacg gcaggagaaa tctccacctt atatgcttac tttgccgaca
     2581 tcacggaacc taatgaaaga accaaagttt ttggatggat gggtgcttta gtcggtatag
     2641 gaacaacgct gggtcctatt atagggggtc tattagctga gttggggaat tctgttccta
     2701 tatttattgg agcactcttt actttcttaa atgccgttta tggctacaca tttatgctag
     2761 aaagccttcc aataaaaaaa cgttcagtag atttaagttt ctcccatgta agaccttttc
     2821 accaattaca acaattattc aagatttcat ccgttattcc tttattgaca gcaggttttg
     2881 ctgtttggtt ggcagctggc tctcttcaat ctatcttttc tcaattttcg attgacgcct
     2941 ttcagtggaa ggctgggttg gttggtttaa gcttctcttt gattggaatc ttggatactg
     3001 tctcgcaatt gttcattatg cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa
     3061 gaatagggat gttcagtgaa atcctcgcct atctattcat cacattatct ggaattcttc
     3121 tactgccgat tttgttttta tttgggatta tctgttatgg tttcggtgat tctattttta
     3181 ctcctgtatt caatggccaa ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa
     3241 tgggcggtac ccaaagtata caatctctat cgcgcgtcat cggcccgcta atcgcaggac
     3301 agctttatgc tgcagcacca tttcttccga cggtattcgg atttttgctt atgatttttg
     3361 cctattatca ctacaataaa aaatagtagc ttcatttgct acttcgtata aattctctgt
     3421 atcgaccgat aaaacgaaat actgtgtcaa attcttcttt tgacatattt tcaaatacag
     3481 tagcatctcg ctgtacccat ttttgatgga gttgctcgtg tctattcctt attttttgtc
     3541 cgattggcgt caattgaaaa tagatttctt ttttattctc ttcgctttga taagtgttta
     3601 tgatccctct agctatcagt ttttttgtta gcttactgat ggctcctcta gtcatgtaac
     3661 tagcattcgc taattttgtc acgttaggat cagggatttt cccgatatgt tcaatcactt
     3721 ctagttcatt tagagtaaat cctgcaaatt ctttttccat ttctggttta ttacgatgaa
     3781 taatctcatt agctaaatcc agatattctt ctattagcag ttgtctttta tccatttcta
     3841 caccttcttt ttctttcatt atattgtttt tttgtttcct attcaacaaa gataaaaata
     3901 ttttgtagga aaacccaatc tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa
     3961 cccctttttc tgattaaaag atagtaaaga gacagtcatc ggtaattgtg atgatttttt
     4021 gtcatcactt aaggatgagc ggccttttca aaaactgtct ttaaagatca tcatcagttt
     4081 ctagcacata tggctgacct tcatatgtgt actctgtatt caatgtgccg tcattttcat
     4141 ctgagtagat gcgtctactg aaaggaaaac gatgagaaac attatctttg gcgtcatttg
     4201 ctggcatcgt gtaatattcc tcttgcgttt cattcaactg agagagtact tgatctgcca
     4261 actcatcaat attgatcgtt aatttcttcg aagcactttt ataatcttct aaaaatgcat
     4321 cttgttggac aaaatattct ttattcatct tctttctcac ctttcttttc aaaatcaaat
     4381 aaagctgctt gttcttttat gtaacagctt tatctttgct gcttattgtg acaaaaaatt
     4441 cgaagaaaga caactaattg tacttttcac aaaaaaagac agagggtcgt gacaaaattg
     4501 tgtcacaacc ctttttcttt attgtgcagt ttcgatttcg cctagcgctg cttgtgcaag
     4561 taggttcaag taattccatg ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc
     4621 gagatcttct acagtcattt tattttgaac agctaaagac atggtatttg ctgattgtgt
     4681 aatgtcgtat ttggacatga actgagcacc tacgatacga ttcgttcctt tttcatagac
     4741 tagctccatc attacttttt cagtagttgg catgaactct ggacgataat tatcttcaaa
     4801 tacagtcgct tggacttcta gatcgttcaa agttgcgctt tctttggtca cacctgtcga
     4861 accgatcgtc cagccaaata ggtaaagtcc tgaagttcct tgagtaccac gatacgccat
     4921 tttatgatct gtcaagttat ttcccacaag caaaccttga cgaacggcat ttgttgccaa
     4981 aggaatatag ttttgcgtgc cacttggatt gtagttgact actgcagagt ctccagctgc
     5041 aaaaatgtct ggattacttg tctgcatgta ttcattcact ttgattgccc cgtttggcag
     5101 tgtatctact tttccttcga tcagttttgt atttggacgg aagcccacac ataaaatgac
     5161 catatctgta tcaaatgtat ctgttggtgt agctacttgt ttcacttttc cttcttcatc
     5221 tgtgataaat tcagtaacat tttcgcctag cgcaagagta acaccgcggt ctgtcaattc
     5281 tttttctaag atatctgtga atggtttatc tagatattta ttcaagatgc gatctaatcc
     5341 atcaatcaat gtcacttgtt ttccagattc gacaaaagct tctactaatt caataccgat
     5401 atatccacca ccaacaatta ctacacgttt tgcttctttc gcttgtgcaa taatttcatt
     5461 tgcttgattg tagtttttgc ataggagtac atttttcgca tcgattccac taatcggtgg
     5521 gataataggc catgaaccag ttgtcatcac taatttatca taagaaaccg tttctgtttc
     5581 tcctgttttc agatcttttg ccgttactgt tttattttct gtatcgacgt ctgttacatc
     5641 gtgttccatt ttaactgttg ctcctaatga agataattct tcaggatttg agtagaacaa
     5701 gcctgctggg tcctttacta ctccgccgac ataaagtgcg ataccgcaag ataagaaaga
     5761 aatattgtca tttctttcat atactgtcac ttctgcttct ggatgatttt ttaaaatact
     5821 ttttacagct gcagtacctg catgagtaca gccaattact actactttca tttatggtcc
     5881 ctccgattaa cattttgtcc ttcgacaaaa ctactataac agaaataaaa cggatacaac
     5941 agtttaatgc ttgtgaattc acaaaggttt gtttgtagtt tcattccttt tcaagcataa
     6001 gaacccaata tctacaagat tcttatttta ataatattca cttttatatc taagattgtg
     6061 aataaaagac aaaaaaataa aaaagctagc gtttttttta cacagctagc tttttttagg
     6121 tataacactt gtagaaactg tttttacatt tgcgcctttt ttgctgactc tttacaatcc
     6181 ggacagagtc catacaattc caaccgatgt tcattaatct caaaatcagt cagtctgctt
     6241 gcagccattt ctatatcttc aagccctggg taatgaaaat caacaatctt accgcagttt
     6301 tcacaaatta catgataatg acgctttgaa ctgaaatcaa atcggctaga agcatcgccg
     6361 tacttcattt cttgaacaaa tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc
     6421 gcaacactca tatttggaaa ccgatgttca agtgctttat aaatctcatc agccgttgga
     6481 tgggaatgat gttcaatcaa atattctaaa accgcgtacc gttgaggtgt gattcgaatg
     6541 ttctcttttt ttaacttttc gataacctct tcgactaact tattttccat gtaaacaccc
     6601 cacgttgtta ttctcgtctt atcatactat ttttacattt taaaatccac ttaacatgaa
     6661 aagaaaattc aattttattg tattttaatg aaaaaagacg taaaacagtc gcgttcacta
     6721 ttctacgtct ctattcaaat aatttttctc atcataaatt tttgtgtgtt cttttcatct
     6781 ttaaaagttt tttacataaa cctatctgga gaaaaaccaa gtgccttata tctcttggta
     6841 aagggctgta gataaataac gttcaccatt gtcgggaacc acagctaata ccttcttgcc
     6901 ttttcctaat tcttttgcga cttttagcgc ggcagcaatt gcagcgccag atgagatgcc
     6961 tactaaaatc ccttcttttc gcccaacttc tctagctgtt tccatcgctt cgtctccact
     7021 aatagaaagg actttatcat acacagaagt atccagtgtt ttaggaacaa aacctgtacc
     7081 gattccttgg attttgtgag gtcccgcttc tttcccttct aaaatagcag attctgctgg
     7141 ttctaccccg attaattcga ttttcggata cacacgtttt aattctcggc cagcgccagt
     7201 gatcgttcct cctgtaccga tacctgcaac aaacgcgtcc aagccattta ctccaaatgc
     7261 ttgatgtatc tcaggaccag tcgtcttctc atgcacaaga ggatttgctt cattttcaaa
     7321 ttgtaatggt aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc tgattgatcc
     7381 agaaatacca tccgcaccag gagtcaaaat caactctgcg ccgtaccctt tcatcaataa
     7441 acgtctttcg atactcatcg tctcaggcat cacgatgatt actttatatc cttttgcaac
     7501 tcctaccatc gacaatccga ttcctgtatt tccagaagta ggttcaatga tagtatcacc
     7561 tggtttcagt agaccatcgt gttctgcttt ttctatcata cttaaagcaa tacgatcttt
     7621 tacacttcct cctggattaa aaaactcaag ctttacaaaa acatctgctg aatcttctgg
     7681 tacgatttta tttaatttga cgattggcgt tttgccaatc aattcggtga ctgagttata
     7741 gatagctgtc atcttcaatt cctccttgtc tttgctcttc ttacttatca taatacaatc
     7801 tgttcattta tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa gatataccat
     7861 atatggtaat
//
LOCUS       DACDSR010000106         7856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDSR010000106 DACDSR010000000
VERSION     DACDSR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.107"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IWK15_002438"
     CDS             complement(<1..179)
                     /locus_tag="IWK15_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9547977.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IWK15_002439"
     CDS             complement(192..1295)
                     /locus_tag="IWK15_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9547978.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IWK15_002440"
     CDS             complement(1385..2320)
                     /locus_tag="IWK15_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9547979.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IWK15_002441"
     CDS             2622..4556
                     /locus_tag="IWK15_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9547980.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IWK15_002442"
     CDS             5013..5429
                     /locus_tag="IWK15_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9547981.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IWK15_002443"
     CDS             5443..6009
                     /locus_tag="IWK15_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9547982.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IWK15_002444"
     CDS             6032..7018
                     /locus_tag="IWK15_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9547983.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7856)
                     /gene="scm"
                     /locus_tag="IWK15_002445"
     CDS             complement(7173..>7856)
                     /gene="scm"
                     /locus_tag="IWK15_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9547984.1"
                     /translation="SDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDT
                     TSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTT
                     STTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHAS
                     VSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRIT
                     FMSYKKSKR"
BASE COUNT         2230 a         1503 c         1638 g         2485 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgtgtc cgtgctatca cttgtggtag aggtagtatc tgtactgtcg
     7741 cttgaagctg acgtgtccgt gctgtcactt gtggtagagg tagtatctgt actgtcactt
     7801 gaagctgatg tgtccgtgct atcacttgtg gtagaggtag tatctgtact gtcgct
//
LOCUS       DACDSR010000107         7693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDSR010000107 DACDSR010000000
VERSION     DACDSR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.108"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..787)
                     /locus_tag="IWK15_002446"
     CDS             complement(59..787)
                     /locus_tag="IWK15_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9547985.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="IWK15_002447"
     CDS             complement(800..2080)
                     /locus_tag="IWK15_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9547986.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="IWK15_002448"
     CDS             complement(2221..2823)
                     /locus_tag="IWK15_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9547987.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="IWK15_002449"
     CDS             3068..3274
                     /locus_tag="IWK15_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9547988.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="IWK15_002450"
     CDS             3586..4305
                     /locus_tag="IWK15_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9547989.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="IWK15_002451"
     CDS             complement(4386..5069)
                     /locus_tag="IWK15_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9547990.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="IWK15_002452"
     CDS             5287..7473
                     /locus_tag="IWK15_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9547991.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2759 a         1293 c         1445 g         2196 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
     7621 aagaattttg tcacacctcc cttatttatt ttgaaaatga gaatactatt atcaaaatag
     7681 agaatatata gaa
//
LOCUS       DACDSR010000108         7634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDSR010000108 DACDSR010000000
VERSION     DACDSR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.109"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWK15_002453"
     CDS             <1..152
                     /locus_tag="IWK15_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9547992.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWK15_002454"
     CDS             complement(230..763)
                     /locus_tag="IWK15_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9547993.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWK15_002455"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWK15_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9547994.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWK15_002456"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWK15_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9547995.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWK15_002457"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWK15_002457"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9547996.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWK15_002458"
     CDS             complement(4488..5066)
                     /locus_tag="IWK15_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9547997.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWK15_002459"
     CDS             5388..5858
                     /locus_tag="IWK15_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9547998.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWK15_002460"
     CDS             complement(5966..7192)
                     /locus_tag="IWK15_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9547999.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2210 a         1535 c         1292 g         2597 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgat
//
LOCUS       DACDSR010000109         7608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDSR010000109 DACDSR010000000
VERSION     DACDSR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.110"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..710
                     /locus_tag="IWK15_002461"
     CDS             <1..710
                     /locus_tag="IWK15_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9548000.1"
                     /translation="MFAGLAISGIVTFFILTPKYSSQAQLIVTLPQSDTANVNDVNTN
                     LQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTAEQIKDAISVNQAENSQMFSIQAIS
                     TNAVAAQQIANTTSQVFQENAKDVMNVDKISIISGAVANTTPVSPNNKLNLMTGLMLG
                     MMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILGTVPEMSAKELNAGIRKKGPAQTQP
                     KATNDKTAPSRRSRSRV"
     gene            722..1420
                     /locus_tag="IWK15_002462"
     CDS             722..1420
                     /locus_tag="IWK15_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9548001.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            1448..2212
                     /locus_tag="IWK15_002463"
     CDS             1448..2212
                     /locus_tag="IWK15_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9548002.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            2256..3707
                     /locus_tag="IWK15_002464"
     CDS             2256..3707
                     /locus_tag="IWK15_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9548003.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            3739..4875
                     /locus_tag="IWK15_002465"
     CDS             3739..4875
                     /locus_tag="IWK15_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9548004.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            4875..5981
                     /locus_tag="IWK15_002466"
     CDS             4875..5981
                     /locus_tag="IWK15_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9548005.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            5987..7009
                     /locus_tag="IWK15_002467"
     CDS             5987..7009
                     /locus_tag="IWK15_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9548006.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            7031..>7608
                     /locus_tag="IWK15_002468"
     CDS             7031..>7608
                     /locus_tag="IWK15_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9548007.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILS"
BASE COUNT         2763 a         1046 c         1586 g         2213 t
ORIGIN      
        1 caatgtttgc tggcttggcc atctcaggaa tcgtaacatt tttcatcttg acaccaaaat
       61 acagttcaca agcacaattg atcgtgacac tgccacaatc agacactgca aatgtcaatg
      121 atgtcaatac gaacctgcaa atgatcaata cgtataaaga tatgatcgtc agtgatttag
      181 tgctcaatga agtcaaagat cgtttagaaa cagaagacaa tgtgaaaatg actgctgaac
      241 aaatcaaaga tgccatctca gttaatcagg cagaaaattc acaaatgttc tcgattcagg
      301 caataagtac gaatgcagtg gctgctcaac agatcgctaa cacaacttca caagttttcc
      361 aagagaacgc aaaagatgtg atgaatgttg ataaaatctc gatcatttca ggtgcagttg
      421 ccaatacgac acctgtttcg ccaaataata aattgaattt aatgactggc ttaatgttag
      481 gaatgatggt aggtgtaggt ctggcattct tattggaatt actcgatcgg acagtgaaag
      541 atgaaaaata tgtgacagat acattaggat tcccaatctt aggaacagtt ccagaaatgt
      601 cagcgaaaga attgaatgct ggtatccgta aaaaaggacc agctcagaca caaccaaaag
      661 ctacaaacga taaaacagcg ccatcacgca gaagccgctc aagagtctag gaggaaagaa
      721 aatggcacga acacagaaac aaaaagcagc ccaaactcat gcagtcagtt tggtcacact
      781 caccaatccc tcttcaccaa tcgctgaaca atatcgtacg attcggacga atatccagtt
      841 tgcctccgca gctggtcagc aaatcaaaac gattgttgtg acttcatcag gacctggaga
      901 agggaaatcg accactgcag caaacattgc cgttgttttt gccaaatctg gtcaacgggt
      961 gctgctagtc gatgcagact tacgtaagcc cgtcatctat aaaacgttca agttgaacaa
     1021 cgcaagcgga ttaagtactg ttcttagttc atcaggaagt gtagcagatg agatccaacg
     1081 aacaccagtc gacaatttgt cgattctgcc aagtggacct aaaccaccaa atccatcaga
     1141 actactaagt tcgccaagaa tggatcagat tttagcagaa gcaagacaac tatttgatgt
     1201 agtgatcttc gacatgccgc cagtcgttgc cgtaacagat gcacagatca tgtcttcaaa
     1261 aacggatggc accttattgg tcgtacggga aaatacgtca cgaaaagaat cattgactaa
     1321 agcaaaagaa ctgttagata tggtacaagc acgagtctta ggtgtcgtat acaacggagc
     1381 agaacacagc aaagatgctg gttattacta cggaaattaa ggaataagaa gagaggagac
     1441 aagagaaatg atcgatctac attgccatat cctaccagga atcgatgacg gggcagaaaa
     1501 cttagaagca agtattgcaa tggctgaaaa agcgattcag caaggcatta cgcatatttt
     1561 atgcacacca caccacaata atggaaaata cagcaatgaa aaatcacaag tcatttctct
     1621 agtcgcttcg ttacaagccg aattagagaa aagacagttg cctttgacgt tattggaagg
     1681 acaagaagta cgtattaccg gaacgctcat cgaagacatc caccgagatg aaattttatt
     1741 tacagattta gatgatacat atcttctgat cgaatttccg acactggaag tgccgttata
     1801 tgcagaacgg cttttttttg agttgttaga actaggaaag ataccagtga tcgtccatcc
     1861 agaacgcaac gcgcattttc gagaagaccc aaatcatttg cttccgttcc tagatatggg
     1921 atgtttggcc caacttactg cgccaagtta tgttggtgta tttggcaaag atatccagaa
     1981 aacagccaaa acaatggtca aacataatct cgtgcagatg gctgcttctg atgcacacgg
     2041 ggtctctaag cgcacctttt acttaaaaga gtgctatgag cagattgcca aggagtttgg
     2101 taaggaaaaa gtagtacaga tgaaacaagt cgcaaaggat ctgatcaacg gggatgagat
     2161 caattatcca atctatgaag aggtaaaaaa gaagaagttt ggtttatttt agttctttat
     2221 tcgtagataa aaaggaaatg aggagagagt gagagatgta tagaaaaaac tcaagtggtt
     2281 ggtctaaaca tattgatttt attttattag acttattttg tgtccagctt gctttttata
     2341 tatcatatgt aatgcgacag ggtgactgga atccgtatgt gattcctatt tatcgccaca
     2401 tggcaatttt tatagaactt gagaatctta cgattatatt tttatttgag gcatataaaa
     2461 atgttttaaa aagaggatat tataaagagg ctgtagcttc tataaagcaa tcttttatgc
     2521 tgttacttgt gtgcagttta taccttatta caatgcagga tggaaatgat tattccagag
     2581 tttctttatt tataatgggg gtggtgtatg gattattaac atatataatt cgtatacttt
     2641 ggaaagggtt gttgcatcac cgcatgaaaa acggtgaaga tgtatcgtta gttattgtaa
     2701 catctcaaaa gattgcaaaa aatgtagttc gtaatcttaa agagaagaat tatggcatgt
     2761 ataaacttag tggtgtgatt gtagtagaca gagatatgta tggaactacc atcggaggtg
     2821 tcccagtagt tgcaaatgct gataatgcag cggaatatct tttacagaat tgggtagatg
     2881 aagtattcat caatgttgac gaaagtgttc catatcccaa agaattaatc gccagatgtt
     2941 cggaaatggg acttgctgta catatgaatc ttacaaaagt gtctgatagt acacgtggaa
     3001 aacaatttat tggccgagtt ggtgattata ctgttattac atctagtatt aatgttatga
     3061 cgatgaggca agcatttata aagagaacga ttgacattct tgctggaatt attggatgta
     3121 ttgcaacagg tgttattttt atcttcgtag caccagttat ttacatcaaa tcaccagggc
     3181 cgattttctt tgcacaagaa cgtattggtc aaaatggtaa gacatttaag atgtataagt
     3241 tccgtagtat gtatatggat gcagaagaac gcaaagcaga gttgatgaaa gaaaataaga
     3301 tgagtaataa tctcatgttt aagatggatt ttgacccaag aatcattggc aataaaatac
     3361 ttcctgatgg aacaaagaag acaggaattg gacaatttat tcgatctaca tcattggatg
     3421 agtttccaca attttataat gtcctaatgg gaaaaatgag cctggtgggg tttagacctt
     3481 gcttgaagtc agaatatgag aagtatgatt ttcatcatcg tgcgagaatt gcgatgaaac
     3541 ctgggattac tggaatgtgg caggtgagtg gtagaagtga tatcacagac ttcgaagagg
     3601 ttgtaaaact tgatgcagaa tatatcagga attggagtat gggattagat tttagaattt
     3661 tgtttaggac agtcaaaact gtgcttcata gaaacggttc catgtaagat aaagatacta
     3721 tagaaggagt aaaacgtgat ggcaagaaaa aaattaaaaa tctgtatgct aggtcataaa
     3781 agaattccat caagagaagg tggaattgag atagtggtag aagaattggc aacgcgaatg
     3841 gttgcacttg ggcatgaagt gacttgctat aatcgaagtg gtcatcatgt tagcggaaag
     3901 gaatttgacc agcataaaaa taaagaatat aaaggtgtaa aattaaagac tatttttaca
     3961 ttaaatataa aaggaattgc agcaatgagt tcttcagtat ttggtggaat tagagcagca
     4021 ttcggaaaat atgatgttgt tcattttcat gcagaaggac catgtgctat gttgtggttg
     4081 ccaaaacttt ttgggaaaag atgtgttgca acaattcatg gtctagatca tcaaagagaa
     4141 aaatggaaca agcttgctag tacatacatc atgttagggg aaaagtgtgc tgtaaaattt
     4201 gcagatgaaa taattgtact tagtgaaagc gtccaaaatt attttgaaga tatatatgga
     4261 agaaaaacaa ggtttatacc aaatggcgtt aaaaaaattg aaataaaatc agcaggattg
     4321 attacagaaa agtatggatt aacaaaagat tcttatattt tgtttttggg gcgattggtt
     4381 cccgaaaaag gaattcggta tctaattgag gcatttaaag atgttcagac agataaaaaa
     4441 cttataatag ccggaggaag ctctgataca gatgaatttg caaatgaact aaaggaatta
     4501 gcaaaaggtg atgagcgaat tatatttact ggatttgtac aaggacagga attagaagaa
     4561 ttatatagta atgcgtatat ctatacactt ccaagtgatt tagaaggaat gccattaagt
     4621 ttgttagaag caatgagtta tgggaactgt tgtatagttt caaatatctc agagtgtacg
     4681 gaagtagtag aagacaaggc tatgattttt aagaaatcgg atgtaagtga tttaaagata
     4741 agattagaag aggcatgcaa tcaatcggaa atggttaaag ttttgaagaa tcaagctact
     4801 gagtttattt gtagtaaata taattgggat aaaatcgtac aagaaacatt aaatttgtat
     4861 aggggaaata tataatgatt cgaatattac agtgtgtgaa tgatatgcat cgtgcagggt
     4921 tggagacgat gctaatgaac tattatagga atatagatcg aactaaaatt cagtttgatt
     4981 ttttgacaca tcgtccatat aaaagtgatt atgatgagga aataataagt cttggaggaa
     5041 aggtctatta tgcaccgaga ctttatccac aaaattatcc taaatatttt aaatggatgg
     5101 aacaattttt taaggatcat ccagaatatg agattgttca ttctcatatt gatgcgatga
     5161 gttatttacc tttaaaagct gcaaaaaaag ctgggattcc gatacggata gcacatagcc
     5221 ataatacatc gatagataga gattttaaat atcttttaaa gcaatattat cgatgtaagt
     5281 taacgtctgt tttgacacat gaattttcat gtggaaaagt agcaggtgat tttttgtttc
     5341 gaaatgataa attcactttg attccaaatg cagtggatgc taaaaaattt tattatgatg
     5401 aaagtattag aaatgatgta cgaatgaaat taggaatttc aagtcagacg tttgtaatgg
     5461 gacatattgg aagaatttca tattctaaaa atcatcgttt tttgattgaa atttttaaag
     5521 aatttcacgc tattaagaat aattccgtat tattaattat tggaactggt gatatggaag
     5581 aagaaataaa aaattatgca aagaaaagtg gcatagatga tgatattaaa tttttaggaa
     5641 atagaaatga tatagaaaaa ttttatcaag catttgacgt attaatgttg ccatcattat
     5701 ttgaaggggt tccgttagtg ggtattgaag ctcaatttgc agatttaccg tgtttctttt
     5761 cggagaaagt tcctacagaa gttgctttca gtgataagac caatttcata agtttaaatc
     5821 aatcggcaaa agaatgggcg agagagattg caaatgtaga tatctctcat cgagattcag
     5881 aacgttcgat tttaataaag gcagattata atattcaaac agcatacaaa atcttagaat
     5941 caaaatatta tgagttattt gaattaatac aaagaggtta aagaatatga cacagaaaca
     6001 ttcaagtata aaaagaatta tacatcgcat atccgaagaa ttgaatgcaa caagacgaat
     6061 tgcaaagata acatcttgga aggaagcatt tgccacgtta caagctaaaa ttgatattca
     6121 agtcatgaat cgtaatggat ataatgaaag taaaaaacag aaggaacatt tattaaaaaa
     6181 acatgaggta atgctgaagt attatgaaaa gactttttca gattttttga aaacatatga
     6241 ttatagtcaa aatcaaataa ttaatcaatc aaaatcacca tattctaact gcatttgggt
     6301 ttgttggtgg cagggattgg atcaatcgcc ggaaattgta aaagcttgtg taaaatcaat
     6361 aaaacgaaat gcagggaaac atactgttat aattttaaca gaagataatt atagacaata
     6421 tgttgatata ccaaaatggg tggaagaaaa gaaaaacaaa ggaatcatta cgagaacaaa
     6481 ttattctgat ttattaagat tatctttgct agcaaaacat ggaggtttgt ggttagacgc
     6541 cacattttat tgtacaaaac caataccaga tgagtatttt gatatcgata tttggtctat
     6601 taggaggcct gattatgctc acgcatccgt agcatcagga tattttgcgg gttattcatt
     6661 agcatgtaat caagagtcta gatggatgtt tgctataatt agagactttt ttttaaatta
     6721 ttggaaaaat aatgatatga tggtggatta tttgatgata gattatatga ttgttttagc
     6781 acagagaata aatcatgaaa tagcacagac atttgataaa atacctagta acaatccatt
     6841 atgtgacgaa ttgattaaaa atttaaataa tatatatgat gaaaaagtat ggaacttatt
     6901 aaagaaagat actttactgt ttaagttgac atggaaacaa tcttttaaaa agcaaaaaga
     6961 tgataaagtt actttttatg ggatgttggt agaaaacaaa ttattataaa tattagaggt
     7021 ataaaaagtt atgaaacaca aggtaagtgt gatagtacca atttacaatg ttgaaaaata
     7081 tttaagaaaa tgtgtggata gtttattgag tcagacatta aaagagatag aaatcgtatt
     7141 agttgatgat ggttcgccag atgcttcggg ggaaatagca gatgagtatc aaaaaaaata
     7201 ttctaatgta aaaacaatac atagagaaaa tggggggtta ggaccggcaa gaaatacagg
     7261 aattgagaat gcgacaggag aatatgtggc ttttcttgat tcagatgatt gggtagaaag
     7321 tgatatgtat gaaaaattat acttagttgc gtccaaagaa aatgctgata tagtagttag
     7381 tggacattgt gattttgcga atgacaaagc tatggtgaaa aagcaacatc ctttgagtgg
     7441 gaatgtatat gatacgccta atagtattaa aaatgtacga aaaaatttat ttggacatag
     7501 tccaaaagat caagaagtag aggccttccc gatgtcagta tgtatgtctt tgtataataa
     7561 aaatctgcta gataaatata gactaagatt tgaaaaaatt ttatctga
//
LOCUS       DACDSR010000110         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDSR010000110 DACDSR010000000
VERSION     DACDSR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.111"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWK15_002469"
     CDS             complement(138..428)
                     /locus_tag="IWK15_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548008.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWK15_002470"
     CDS             complement(546..806)
                     /locus_tag="IWK15_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548009.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWK15_002471"
     CDS             complement(943..1356)
                     /locus_tag="IWK15_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9548010.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWK15_002472"
     CDS             complement(1812..2297)
                     /locus_tag="IWK15_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548011.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWK15_002473"
     CDS             complement(2436..2651)
                     /locus_tag="IWK15_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9548012.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWK15_002474"
     CDS             2875..3675
                     /locus_tag="IWK15_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9548013.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWK15_002475"
     CDS             3694..5562
                     /locus_tag="IWK15_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9548014.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWK15_002476"
     CDS             5555..6046
                     /locus_tag="IWK15_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9548015.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWK15_002477"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWK15_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9548016.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWK15_002478"
     CDS             6606..>7600
                     /locus_tag="IWK15_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9548017.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDSR010000111         7411 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDSR010000111 DACDSR010000000
VERSION     DACDSR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.112"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..351)
                     /locus_tag="IWK15_002479"
     CDS             complement(16..351)
                     /locus_tag="IWK15_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9548018.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(335..607)
                     /locus_tag="IWK15_002480"
     CDS             complement(335..607)
                     /locus_tag="IWK15_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9548019.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1058..1861)
                     /locus_tag="IWK15_002481"
     CDS             complement(1058..1861)
                     /locus_tag="IWK15_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9548020.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1839..2963)
                     /locus_tag="IWK15_002482"
     CDS             complement(1839..2963)
                     /locus_tag="IWK15_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548021.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2979..3794)
                     /locus_tag="IWK15_002483"
     CDS             complement(2979..3794)
                     /locus_tag="IWK15_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9548022.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3784..4665)
                     /locus_tag="IWK15_002484"
     CDS             complement(3784..4665)
                     /locus_tag="IWK15_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9548023.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4741..6030)
                     /locus_tag="IWK15_002485"
     CDS             complement(4741..6030)
                     /locus_tag="IWK15_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9548024.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6258..6902)
                     /locus_tag="IWK15_002486"
                     /pseudo
     CDS             complement(6258..6902)
                     /locus_tag="IWK15_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1590 c         1136 g         2407 t
ORIGIN      
        1 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
       61 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
      121 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
      181 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
      241 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
      301 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
      361 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
      421 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
      481 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
      541 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
      601 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
      661 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
      721 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
      781 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
      841 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
      901 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
      961 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     1021 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     1081 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     1141 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     1201 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     1261 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     1321 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     1381 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     1441 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     1501 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     1561 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     1621 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     1681 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     1741 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     1801 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     1861 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     1921 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     1981 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     2041 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     2101 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     2161 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     2221 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     2281 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     2341 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     2401 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     2461 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     2521 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     2581 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     2641 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     2701 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     2761 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     2821 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     2881 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     2941 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     3001 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     3061 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     3121 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     3181 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     3241 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     3301 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     3361 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     3421 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     3481 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     3541 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     3601 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     3661 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     3721 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     3781 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     3841 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     3901 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     3961 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     4021 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     4081 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     4141 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     4201 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     4261 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     4321 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     4381 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     4441 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     4501 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     4561 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     4621 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     4681 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     4741 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     4801 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     4861 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     4921 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     4981 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     5041 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     5101 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     5161 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     5221 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     5281 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     5341 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     5401 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     5461 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     5521 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     5581 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     5641 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     5701 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     5761 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     5821 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     5881 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     5941 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     6001 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     6061 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     6121 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     6181 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     6241 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     6301 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     6361 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     6421 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     6481 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     6541 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     6601 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     6661 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     6721 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     6781 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     6841 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     6901 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     6961 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     7021 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     7081 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     7141 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     7201 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     7261 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     7321 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     7381 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACDSR010000112         7283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDSR010000112 DACDSR010000000
VERSION     DACDSR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.113"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IWK15_002487"
     CDS             complement(170..3319)
                     /locus_tag="IWK15_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9548025.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IWK15_002488"
     CDS             complement(3333..4550)
                     /locus_tag="IWK15_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9548026.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="IWK15_002489"
     CDS             complement(4540..6135)
                     /locus_tag="IWK15_002489"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9548027.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="IWK15_002490"
     CDS             6260..7045
                     /locus_tag="IWK15_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9548028.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2132 a         1477 c         1199 g         2475 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aag
//
LOCUS       DACDSR010000113         7216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDSR010000113 DACDSR010000000
VERSION     DACDSR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.114"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(411..626)
                     /locus_tag="IWK15_002491"
     CDS             complement(411..626)
                     /locus_tag="IWK15_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9548029.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(645..881)
                     /locus_tag="IWK15_002492"
     CDS             complement(645..881)
                     /locus_tag="IWK15_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9548030.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            1139..3292
                     /locus_tag="IWK15_002493"
     CDS             1139..3292
                     /locus_tag="IWK15_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9548031.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            3261..4676
                     /locus_tag="IWK15_002494"
     CDS             3261..4676
                     /locus_tag="IWK15_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9548032.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            4709..5743
                     /gene="sppA"
                     /locus_tag="IWK15_002495"
     CDS             4709..5743
                     /gene="sppA"
                     /locus_tag="IWK15_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9548033.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            5745..6266
                     /locus_tag="IWK15_002496"
     CDS             5745..6266
                     /locus_tag="IWK15_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9548034.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            6263..6649
                     /locus_tag="IWK15_002497"
     CDS             6263..6649
                     /locus_tag="IWK15_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9548035.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2524 a         1081 c         1298 g         2313 t
ORIGIN      
        1 ttcatttttt agttaaaaaa tttgtcctgt caagtgcaat cctatgattg tacttgatag
       61 gatttgagat aattactttc ctgtttttat aaatttaact tgcaggaaca ggctttaatg
      121 cacatccgac aagtacatta ccaatacctc ctgtaacggc cgaaataggt cctcctgcag
      181 caccaactac agcgctgcaa gtccttttgt aaaaatatct ctaccattac atttccaaag
      241 aatttttatc tgactttaat aaaagataaa aatttaatat aattataaaa actagcacaa
      301 gaagctttag taaaaagttc tgtccattcc aaacttttga aaagataaag aacataccta
      361 tgttcgtaat cgacacaaac caatcttgag gtttgatatt ttttaattgt ttacgcaccg
      421 gtccacatgc tgaatccagc acctaataaa gctccaccta caccacaagc agctattgca
      481 taaggtcctc cggctgaaca aagttttact cctgctgttg ccccagaaaa agtagtgcct
      541 aaaactccca cggcactccc accttgaata tttttcaact cttctgaaga taatataaat
      601 gttgtttttg tgtttttaat atgcatacta cttattctcc ttttttattt ttttcgagca
      661 tcccagccag ctgcagcacc tccagcgaca gctccatata accctccaac aacaccgcaa
      721 gctggtccgc aaattgctgt tcctaaacct gcaccaccta ctgcccctgt ggtaagtcca
      781 cctacaactt gccccaattt tccccaagca ttattgcctc caccaacagt ttgtgcattt
      841 ccacaatact tagttccttt acattttgca tgtttaaaca cgcttcttta aataatattt
      901 tttacggtgc tactgtaaca tacgtatttg attaacagta aaaatcttcc tgaaacttat
      961 caagtttatt cttgaatagt aaaatttcca aaataaattt gattcaggaa tgaaaaagtt
     1021 agtgttcaag aactgttttc aaagtttttg tggaattatg agacaatatt ttcactgtat
     1081 taaagctata ttgagaattt ttctataaaa attttttaga aggagttgtg ttagataagt
     1141 gataaaatca tttccttttg tgcagcaaca agatgaaaaa gattgtggtg ttgcttgcct
     1201 ttcgatgatt ttaaaatatt ttaaaactga tatcccgatt cataaattac gggacctatc
     1261 tgggactgat ttagagggta cttcagcatt tggattgaaa aagacatttg aaaaattgtc
     1321 ttttgattgt atggctattc aagcagatag tgagatctgg aaagaaagag atcttatctt
     1381 accattaatt gcacatatat tggttgataa cagctatatg cactacgttg taatttataa
     1441 aacgaaggga aacgtactat ggattgcaga tcccgctaat ggaaaaaata gaaaaacgat
     1501 tgaagcgttt caaaaagaat ggacaggtat tcttttatta cctatgccta gagaaactta
     1561 tattcctatt aaagaaaaag tagctggatt aagttctttt ttgcctataa tttggaaaca
     1621 aaaaggatta gtattccaca tcgttttagc atcgattttc attacgtttt ttggcatagg
     1681 aagctcatat tattttcaag gtattcttga ttttttcatc cctaatcaag cacgttcaac
     1741 tttaaatata atttccattg gcttgatcgc tgtctattta tttcgggtat tgtttgaata
     1801 tagtcgtagt tatcttctta ttattttagg acagcgaatg agtatggcaa ttatgcttca
     1861 gtatttcaaa catgtgttaa ctttaccaat gaattttttt gctacgagaa aatcagggga
     1921 aattatttct cgttttcttg atgccagtaa gattattgat gctttagcaa gtgctacgtt
     1981 atcaatattt ttagatattg gtatggtgtt gcttgttggg gttacgttag ctattcagaa
     2041 tagtacgttg tttttaatca ctctatgctc tttgcctttt tatgtagttg caatattcgc
     2101 tttcgtgaaa agctatgaaa aatcgaacag ggaagaaatg gcagccggtg cagctgtaaa
     2161 ttctagtatc atagaaagtc ttaaaggaat agaaacaatt aaagcatata gcggtgaaga
     2221 gaaagtatat aaccgtatag accatgaatt tgttaaatta atgaaaaaat catttcgtac
     2281 ggttacgctt gataatattc aacaaggaat taaacatttg attcaattaa ttagcagtgc
     2341 attaatttta tggctaggtt cttattttgt tatggataat agaattagtc taggtcaact
     2401 tatcacatac aatgctttac tagtattttt gacagatcct ttacaaaaca ttattaattt
     2461 acaagtgaaa atgcaaacgg ctcaagttgc aaataatcgt ttaaatgaaa ttttcgcgat
     2521 tgattcagaa aaatcgaacc agtcttttga atatatgctt tctgaacaat tatttcaaca
     2581 agatatcact gtaaaaaacg tttcattctc gtatagcatg aaggccccaa cgttgaacga
     2641 aatttcatgt acaattcctt cgcacagtaa aacggctata gttggagtta gtggttctgg
     2701 aaaatcaact cttgcaaagt tatttgttcg tttttatgaa gcatcagaag gatcgatcca
     2761 ttatggaaac attaatcact tagacattcc ttttcagata ttacgcgatc gagtgacata
     2821 tttgcctcaa gagtcatttt tctttagtgg aacaatcatt gagaatctta tattcggttt
     2881 agatagccca ccaagtttcg aacaaataat agaagtttgt gagatagtcc aactaaatga
     2941 atttattaat caacagccat tacgttacga gacattgttg gaagaaggtg gaaataatct
     3001 ttcaggaggg caacgacaac gattagcctt agcacgggct ttattaaaaa atccggacat
     3061 attaatttta gatgaagcaa ctagtggatt agataccttg ttagaacatg caattatgaa
     3121 ttatctcctc aatttaaaag aaaaaacgat tatttttatt gcgcatcatt taccaatagc
     3181 aaaagcatgt gatcaaattt tagttctcca tgaagggcat ttggtagaaa aaggaacgca
     3241 cgatgaactt cgttttgccg atggtatcta tcaaaaatta tgggaaatct aggagaggga
     3301 aacaatttat gcagaataat aattggttcg aacattctag tatctatact aaacaacatc
     3361 atagcttcta ccgttgggtg ttataccctg taattctttt tttatgtatt attggattgt
     3421 ttttaacctt tgctaaaaaa gaggttgtta ttcggacaac ggctcaattg aaccccgaaa
     3481 aaatagagaa gttacaagtg ccattagaag ctaaaataat ggaaaacttc ctaaaagaaa
     3541 atcaatttgt tcataaggac gatgtgttgg ttagattgga ttgttcttta atagagaatg
     3601 aaaaggctca gatagaacaa gaaaaccaac gtattactca acaaataaag atggctcagc
     3661 tatttattga aagtataagt aaaggaaaaa atttgttttc aacggatgac agttttggct
     3721 acagtaatca attaaagagc atgttgtcag aaaaagaatc actccgctac gctttgaagc
     3781 aaagtgaatt aaatgatcaa aagcaattag aagtatacga aaagacaaaa agacaactag
     3841 aaaaacaaat tgagagttca gatagtaaat tacaagaatg gcaacaagta caggtagctt
     3901 ggagtaataa tcaatcatta aaagattttt caaaagaaat gatggcaaac tatgagaatt
     3961 ggcaagaaca actaaataat gtttctgatg atcaaaaaaa tcaagtgaaa ctgacaattt
     4021 cagcaagcat aaatgaacaa attgagcaac taaaaaaaga agtagaacag tatcagtcag
     4081 aaaaagctaa attagttaaa ccaactactt ctgagaatga cagaattagt caaacggaaa
     4141 aaggaaagca agagctagaa caagcaataa caactgtaaa acaaacagtt acagagctaa
     4201 atgaaaaaca agagaagaac cagtcagtta tcaaaagttt ggacgatcaa ctttcaaaag
     4261 gaatactaaa agctcctgta tctggaacgg ttcatttaaa tgaagaaaca aagggacaag
     4321 cagaactagc caaaggaaca gtgcttgcag aaatttatcc gatccacgaa aaatcaaaaa
     4381 tgaaatttac agcactttta cctgcgaatg aaagtaccta tattaaagaa gggatgaagg
     4441 ttcattttaa attggatcaa aaaggtgggg ctccgattac gatcgacgga actttagatg
     4501 aaatttcaga aaattctact gccactgaga aaggtgtttt ttatgcagtg aaaggaacgt
     4561 taaaaacaac tcaatcgttt ccgtttcgtt atggattaac tggagagttg tctttaattg
     4621 taggagaaaa aacatattgg caacaaattg tcgatgtgtt tctaaataaa aaataattat
     4681 ttttactaag aatataggag gagtttatat gaataaaaga agatggattg ctgtgggagt
     4741 agcgtgcgga ttattgttac tatccattat cgtcgcgcta attccaggaa aagataaaga
     4801 ggaagcatca aatacaaccc taacaggaat aaataagatt ttttacggat ctaacgaaat
     4861 cacagaagaa acgttagaag aaggcgcatc aaataaaaag attgtcaaac tatccgtcaa
     4921 tggagttatt gcagatacag gtgaaagcaa tctttttagt agggaacaat ataaccatca
     4981 gaatttttta acacagctaa agaaaattca agaagacaaa gcggttaagg gagtgttatt
     5041 agaagtaaat tcacctgggg gaggaatata tgaaagtgct gagattgcta aagaaatggc
     5101 aaaaattaaa aagctagata ttcctatcta tactgcattt aaaaatacag cggcaagtgg
     5161 agggtactac atttctgctg gttcagataa aatatttgct acggaagaaa caacaactgg
     5221 atcaattggt gtcattattt ctggtttaaa ttattctggc ttattagaaa aacttggcgt
     5281 aactgatgcg acgtataaaa gtggtgcatt aaaagatatg atgtcacctc aacacaaacc
     5341 ttcagaggaa gagaataaag ttatacagga atttgtgatg agtgcttatg atcgttttgt
     5401 caatgttgta gcaaaaggaa gaaatatgga tactaatgca gtgaaagaac ttgcggatgg
     5461 aagaatttat gatggtaacc aagcagtaga aaatgggttg gtggatcaaa ttggttactc
     5521 tgaagatgca ttagattccc taaaaaaaga gaagaaatta actgatgcga cggttataga
     5581 atataaaaat gacacaactg gctttgcttc ttcttggtta ggtaataaga ttgctgaatg
     5641 gcaagggcta aaggcaacta catcagatcg tgtaatttca gtttttgaaa aacttggatt
     5701 ttctgatact ccaaaaccaa tgtattacta tggagggcta taaaatgaat aagaagagtg
     5761 gcattgaaac tttgcctaat tatgtatttg cgggattttg gttacgattt tttgcttttc
     5821 ttattgattt aatttgtatt agcatcatca cccatgctac tataggtctc ttttacaaaa
     5881 tcatgggact ggaatctgac aaatccatta ttacttttta tggtatagcg tcattagcta
     5941 tttatttaac ctattttgta cttttgacta agctgaattc aggacaaaca attgggaaaa
     6001 tgatttttgg gataagagta gtgagctttg aggaaaaaga gctaagttgg caaactatct
     6061 ttattcgtga gtgtatatgt aggtttatat taaaaaattt tgtatttcaa attgggtatt
     6121 tggtggctgc tttcacgccc aagaaacaac atattgggga ttatttttct aacacgtcag
     6181 tggttgttat tcgttcatta gaagctatta actatggtca aaaaatttct aattctaaaa
     6241 cacttaatct ggagagaatt aaatgaataa gagaaaaaca gagtttattt tgattttaat
     6301 tagtggaata acagggattt tatgtagtct tttagcattg atgggtggta tgtttctatc
     6361 ttctattgat tcaactactt taaatactac agaactaacc agtaaggata tagatatgtt
     6421 agtcaatcta agcgggaatg ttgttttatt tggaggaatt accctagttt tttcgataat
     6481 gattttcata ataggttttt tagtaagaaa aaatcaaaaa acagtggtct atggtattag
     6541 tttactcatt ttatctatta ttccattttt tcttttaaca ttattgtggg taattcctgg
     6601 agttttaggt attattgcgg gattgatgct tctaataaga aaatcttaat taggcaatgt
     6661 gtagacaacg tgaaacgatt aatcaattaa aagctaaata cgttttgaga aatgataact
     6721 tgtagtcgat ggaagacatg tagataaaaa aactacgtgt cttttattta ttctaatttt
     6781 ttcgaaaaaa agacactctc tgattgttgt attaatggaa aaggggaaag cagcaagtaa
     6841 atttagtttt tctattaaat attcttcact ttttaacctt ttatagtact tagatttaat
     6901 cctatatgtt tagagagttc attggcagca tgattttgca aagcttctcg aattattgtt
     6961 atattagaaa ctttactgtt cataattatg cttctttttt aactcaaagt ccaaagggct
     7021 acagtggact ttgagttaaa aataaaaagc cctagttatt ttgaaaaata aaaaaagaac
     7081 agcgtaaatg ttttcgaaat gctttcgcac caacatttac ctgttccgtg ggtttggcac
     7141 ccaatgggcc gtgaggggct cgaacccgcg acccgctgat taagagtcat taatgtcaat
     7201 ttttaaataa aattaa
//
LOCUS       DACDSR010000114         6817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDSR010000114 DACDSR010000000
VERSION     DACDSR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.115"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..632
                     /locus_tag="IWK15_002498"
     CDS             <1..632
                     /locus_tag="IWK15_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548036.1"
                     /translation="KNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNIQ
                     SIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTN
                     RYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILN
                     EYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            619..2040
                     /locus_tag="IWK15_002499"
     CDS             619..2040
                     /locus_tag="IWK15_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9548037.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            2053..3060
                     /locus_tag="IWK15_002500"
     CDS             2053..3060
                     /locus_tag="IWK15_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9548038.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            3072..4076
                     /gene="neuB"
                     /locus_tag="IWK15_002501"
     CDS             3072..4076
                     /gene="neuB"
                     /locus_tag="IWK15_002501"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9548039.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            4073..5221
                     /gene="neuC"
                     /locus_tag="IWK15_002502"
     CDS             4073..5221
                     /gene="neuC"
                     /locus_tag="IWK15_002502"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9548040.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            5194..5871
                     /locus_tag="IWK15_002503"
     CDS             5194..5871
                     /locus_tag="IWK15_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9548041.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5822..>6817
                     /locus_tag="IWK15_002504"
     CDS             5822..>6817
                     /locus_tag="IWK15_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9548042.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIET"
BASE COUNT         2464 a          881 c         1249 g         2223 t
ORIGIN      
        1 ataaaaatag catcttcaaa aaaaaaattg acgattattt tgtctattgt ccggaattgt
       61 tactttatga tgtaaaaaat ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga
      121 tgaaaaacat acaatcaatc tttgggatag aagaagtttt tgtcggagat aaatgtatct
      181 tttttgatca acctttatta tctgatggtt atggcataga tgaaacagat attttcgcta
      241 aagtaaatag tatattttct ggaaaaaaag aaactttaat taagcttcat cctagagcaa
      301 ggacaaatcg ttatggtgaa aaaaacatat tgaattccag tttaccgttt gaagtcttgc
      361 tacttgaaaa tgacttctct tcaacacttt tgattagtcc tatatctaca atttcttttt
      421 caccatctat gatgtttgaa aagacgatca actctattat gttggctaaa ttaattttaa
      481 atgaatatac agcattatct gaagataaaa aagaaacatt gacaaatatt gttcttttct
      541 gtgaacatta taaccgtcta tcagaagaaa aaattttact cccagaaaac tgggcagaat
      601 taagaaagtt ggtagagtat gggattattt aacacacttt ccaagtcaaa attagttaag
      661 tccagtttct ggtatactat agggaatttt tttattaagg gaatttcatt cataactatt
      721 cctatttttg taaatattac tactcaagca gaatatggtt tgattaataa tttcacttca
      781 tttgtatcaa ttttttcgat tcttgttggg ctaagcctaa attcggcaat taataatgca
      841 agttttgact ataaagatga cataaatgaa tttatgtcat cggtactttc tctagctaca
      901 ctttcgttaa ttgtgttctt attaataggg aacatatatt ttttgttttc aaatgctttt
      961 tttgatatga accaacttgt atttaatttg atgttattac aaagttttgc tagttttgta
     1021 attagttata tccagtctta ttttacgatt aactttaacc attttaaata tttgatttta
     1081 tcttttatat ctactttttt gaatattgca atctctgtag ctttgatgca gacattatat
     1141 tttgatgata aatatgttgg cagagtagtc ggcggaacac tgtcatttgt gttgataggt
     1201 ttagtaattt atttaagaat aatgataaag ggtgctcatt tttttgataa aaaacagtgg
     1261 agatacgctt taaaaatctc tttgccgcta atcccacatt ccttagcaaa tatagtttta
     1321 gcacaattcg atagaattat gattaattct tatttaggtc ctgctgcggg gggaatatat
     1381 tcctacatta gtaacatagg tattattcta agcgtaatat ggatgtctac taataacgcc
     1441 tgggttccat ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt
     1501 agtaactatt atttagtagt ctttactttg attacgatga ttgtaatggt agtttcgatt
     1561 gatttagcaa aatttatggc tccgaaagac tattattctg ggttatcttt agtggttccg
     1621 ataaccttag gctatttctt tcagttttta tatagtttac cagttaatgc tgaattttat
     1681 gagaaaaaaa catcgtttat tgcgattggt acagtagcaa gtgctatagt taatatacta
     1741 ttaaacatga ttttcatacc taggttagga ataattgctg ctggatacac aaccgtattt
     1801 acgtattttt tactttttat ttttcattat aatatcgcta aaaaaattac gaataaacaa
     1861 ttatttgaca caaaaaaaat atgtatcata ataatttttg tatcaatttt ttctttgata
     1921 cttcatattt taatcaatca aatgttgatt cgttacgttt tgacatcatt attgataatt
     1981 agtttagtta tatatttttt aaagattaag aatcgaatta tagatgacta taattcatag
     2041 gaggagatat aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt
     2101 ggaagtgaaa ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt
     2161 agaggtgaag aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga
     2221 tttttaattg gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat
     2281 attatttttc atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa
     2341 gctgtaaaaa caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat
     2401 gtaaaacatt ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt
     2461 gcgacaaagt tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca
     2521 gaaacaatat tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt
     2581 cctcttttta gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg
     2641 acacgattta tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat
     2701 tcaaaatttg gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct
     2761 caatcattaa taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa
     2821 gttgtaggta tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt
     2881 gaacgctcat atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac
     2941 gaactgtaca aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa
     3001 ttaatgagtg gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa
     3061 ggagttttat gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg
     3121 gaagtctaga tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg
     3181 ttaaatttca aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat
     3241 atcagaagga taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat
     3301 tatctcaatc tgattttatt agaataaaag attattgtat acaaaataaa attgattttt
     3361 tgtctactcc ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat
     3421 ttaaaattgg ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg
     3481 ggttgccagt tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag
     3541 attgtttcat agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt
     3601 ctgaagatat taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg
     3661 ggtactccga tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta
     3721 aggtaattga gaaacatttt accttggata aggaactacc tggtccagat cataaagctt
     3781 ctttagatcc tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt
     3841 taggtgatgg tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa
     3901 aaagcctagt ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta
     3961 caataaaacg tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa
     4021 aagttaaaag accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa
     4081 aatacttata gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa
     4141 tttactacaa gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa
     4201 atatggattt actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca
     4261 aagtttgttt ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa
     4321 tggtttcaca aatatatttg aacaaactag ccctgatttt gtattattac taggggatag
     4381 aggagaaatg ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat
     4441 tcacggaggc gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat
     4501 agcatctgtt cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga
     4561 agattcttgg agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag
     4621 attacctagt tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata
     4681 cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt
     4741 agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag
     4801 tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat
     4861 ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat
     4921 gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa
     4981 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
     5041 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
     5101 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt
     5161 gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta
     5221 aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt
     5281 gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa
     5341 atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa
     5401 aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata
     5461 aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg
     5521 gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca
     5581 gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt
     5641 agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta
     5701 ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct
     5761 tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct
     5821 tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt
     5881 atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa
     5941 ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac
     6001 tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt
     6061 aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc
     6121 tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa
     6181 tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa
     6241 ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg
     6301 aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt
     6361 aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt
     6421 taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa
     6481 atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca
     6541 acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg
     6601 acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa
     6661 ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc
     6721 aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa
     6781 cgtaataaat aaactaaatg aaaaaaacat tgaaaca
//
LOCUS       DACDSR010000115         6690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDSR010000115 DACDSR010000000
VERSION     DACDSR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.116"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..835
                     /locus_tag="IWK15_002505"
     CDS             <1..835
                     /locus_tag="IWK15_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9548043.1"
                     /translation="TRIKIIQLLSKKKMNVKDLAKELGVSSAITTMHVKKLEEANIIK
                     TEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGHYTNYAIEPTCGLATIHDF
                     IGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTEDTLEMLEVSVEISSEFPFS
                     NDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPDNLNQYGLLKTIRITKHLT
                     NMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTLFGKGFGNYDQDIKLKVYY
                     S"
     gene            complement(908..1732)
                     /gene="nadE"
                     /locus_tag="IWK15_002506"
     CDS             complement(908..1732)
                     /gene="nadE"
                     /locus_tag="IWK15_002506"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9548044.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1744..3216)
                     /locus_tag="IWK15_002507"
     CDS             complement(1744..3216)
                     /locus_tag="IWK15_002507"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9548045.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3853..4215)
                     /locus_tag="IWK15_002508"
     CDS             complement(3853..4215)
                     /locus_tag="IWK15_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9548046.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4438..5190
                     /locus_tag="IWK15_002509"
     CDS             4438..5190
                     /locus_tag="IWK15_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9548047.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5330..6253)
                     /locus_tag="IWK15_002510"
     CDS             complement(5330..6253)
                     /locus_tag="IWK15_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9548048.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6268..6540)
                     /locus_tag="IWK15_002511"
     CDS             complement(6268..6540)
                     /locus_tag="IWK15_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548049.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2035 a         1339 c         1144 g         2172 t
ORIGIN      
        1 aacaaggatc aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc
       61 aaaagaactt ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc
      121 aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa
      181 aatcgatatt agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat
      241 acctattggt cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca
      301 tgattttatc gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc
      361 ccgcattctc tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac
      421 agaggataca ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc
      481 aaatgacaat tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg
      541 gacgagccct ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga
      601 taatcttaat caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat
      661 gaatggagaa cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg
      721 gcatttgcga atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg
      781 taaaggattc ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt
      841 ttcttttcta ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc
      901 tttattgtta tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg
      961 tcttgttcca ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta
     1021 gataatcatc aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt
     1081 tcccatcttc aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg
     1141 cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc
     1201 caccatcacc aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta
     1261 atacggctcc tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt
     1321 tgatatttcc tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt
     1381 gtgcatcaac agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa
     1441 gagcagcttt tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa
     1501 attgatagtt ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac
     1561 ggcctgctaa agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg
     1621 gatatttttt caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat
     1681 caattgttgg tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc
     1741 ctcctatgct tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat
     1801 tttatggttc cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg
     1861 tttatactct tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag
     1921 tgcaggtaat tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc
     1981 tgtaaagtct cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc
     2041 ttcttcttct cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc
     2101 aaaatttcga gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt
     2161 gcttgataac ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt
     2221 gaatactgca ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca
     2281 tacatcaatt ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt
     2341 cgaagcatag atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt
     2401 agatatatag gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc
     2461 taattctttc gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc
     2521 gactaagaac acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt
     2581 tccataagat tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt
     2641 cccagcacgt acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca
     2701 gactgctgca tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc
     2761 gatgacagat ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt
     2821 caacagtgct gtttctacta attggcagtg cgctaaaggc ccttccactt ggatcaatgg
     2881 ttcatttgca aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa
     2941 attttgtaaa tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat
     3001 atcggaatca ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa
     3061 aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa
     3121 atcagctcga cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg
     3181 cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt
     3241 tttcatttcc tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt
     3301 ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac
     3361 tatcctttct tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat
     3421 ccgagacacg aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt
     3481 cctactacaa tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct
     3541 acaacataat caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct
     3601 tatccccaaa gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta
     3661 tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa
     3721 gatacaaagt aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc
     3781 agtctcgcct tgttttttat tgtatacatt agatatggag caggttacca taaagatgat
     3841 tattagtcgt tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt
     3901 atttggacgt ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac
     3961 tttgacatct tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct
     4021 tgtgattttt ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa
     4081 tagcagttgt gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa
     4141 aaagaattcc actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt
     4201 ttcgattagc ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt
     4261 tatgttacca tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa
     4321 ggatttacct tttatcactt aatagtataa cataatttca tgagaaaaaa atacaatttt
     4381 ttcttgacca tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg
     4441 gagtacacaa taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt
     4501 tatgatgaga ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat
     4561 ggcaaaaaag aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag
     4621 ttggatgata tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc
     4681 aagcatcagc aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc
     4741 caacagacac ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa
     4801 gggtttaaac aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa
     4861 aaatacggaa aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa
     4921 acgtatgaag aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta
     4981 aaagaaccag aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggtta
     5041 tcttacagct ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa
     5101 gccgatgaac ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct
     5161 ttaaatgaaa ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac
     5221 gtaaaatgac ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg
     5281 gtgtctgctc cattctttca caagtttttt catcctactt taaatatatt catcgttgaa
     5341 taaacgtata aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca
     5401 tttctccgca agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca
     5461 gcaaattatc gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc
     5521 ctgctgcaac catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga
     5581 agcctgcttg ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa
     5641 aagatggaaa aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc
     5701 cacttcgccc caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac
     5761 cttctgtaat tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt
     5821 cttcacaaaa aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg
     5881 cacgagacgt aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc
     5941 gctctttttc tttctgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg
     6001 ctgcttcaat gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa
     6061 tcactccaat gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat
     6121 aatcgacttc tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat
     6181 gtccattgat tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc
     6241 gaatgtttgc cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac
     6301 cacagctgga ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg
     6361 ctgcccgccc caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt
     6421 tactatattc ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc
     6481 gacaaacgca taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat
     6541 tgcaagccct ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag
     6601 tttgttttgc caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta
     6661 aattgaatac attataatga tactgtaaat
//
LOCUS       DACDSR010000116         6473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDSR010000116 DACDSR010000000
VERSION     DACDSR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.117"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(362..1144)
                     /locus_tag="IWK15_002512"
     CDS             complement(362..1144)
                     /locus_tag="IWK15_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548050.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1209..2045)
                     /locus_tag="IWK15_002513"
     CDS             complement(1209..2045)
                     /locus_tag="IWK15_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9548051.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2063..3472)
                     /locus_tag="IWK15_002514"
     CDS             complement(2063..3472)
                     /locus_tag="IWK15_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9548052.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3486..5309)
                     /locus_tag="IWK15_002515"
     CDS             complement(3486..5309)
                     /locus_tag="IWK15_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548053.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6152..6463)
                     /locus_tag="IWK15_002516"
     CDS             complement(6152..6463)
                     /locus_tag="IWK15_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548054.1"
                     /translation="MNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQILQEQ
                     YNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYAEVLML
                     N"
BASE COUNT         2000 a         1374 c         1023 g         2076 t
ORIGIN      
        1 tagactgatc gttaacttat tttttatatt tctacatagt tatctttaga accatttcag
       61 atacatatga aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat
      121 aaaatataca aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa
      181 cacttatata tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat
      241 agtcgggctc caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat
      301 gtataagtac ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc
      361 attattcaat aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata
      421 ccacagaata atcttttttc ccattttcaa gtaaccgaag tggaatgtct ttaccaactt
      481 gctctgtcac tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag
      541 catctgtaga aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct
      601 tccaagtagg tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg
      661 tcttcccagt atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg
      721 tctgctccgt aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac
      781 ttatatattg tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt
      841 cactataacc agttctttca atagagactt caatgtattt cacactatca tactcaaaag
      901 aaaaatagac atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta
      961 cttgagacct tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct
     1021 tattattaat ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac
     1081 actttaccac ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt
     1141 ccaaggaatc aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt
     1201 ttcttcattc agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt
     1261 gctgaggcac ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct
     1321 ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc
     1381 ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc
     1441 gaatctttat caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt
     1501 agtgctctta ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca
     1561 atgtgaaacg caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg
     1621 atcaattgga tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca
     1681 atcagcaggc gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag
     1741 tgatcaacca aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca
     1801 taatcgataa ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta
     1861 atgtaatcct tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg
     1921 tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt
     1981 acttctttgc cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact
     2041 ctcattagat tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc
     2101 aaaagaagct ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa
     2161 catatataaa accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg
     2221 accaaggtaa ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa
     2281 tatgtttggc tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta
     2341 tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca
     2401 atttcctcaa tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat
     2461 ctacataagg attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat
     2521 cccccgctct agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg
     2581 aataataatt aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg
     2641 cctcaatttt aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt
     2701 ttccccgaac tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg
     2761 caattgcatc ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac
     2821 cgatttttcc tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata
     2881 aaacatggtg attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca
     2941 aaccaatcgc tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt
     3001 tcaccttgtc accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag
     3061 ttgctcgatt taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca
     3121 tggtgacaat cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag
     3181 aaattccctt ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg
     3241 aaaagcgaaa agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat
     3301 ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa
     3361 taatagacgg ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg
     3421 aggcacccca taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc
     3481 tctccttaac taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta
     3541 attcctgcgt aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg
     3601 tttctaatgg cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct
     3661 gcttttacaa atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg
     3721 atcaataatt caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata
     3781 gtaatgactt ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg
     3841 ccctttccca atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg
     3901 gttcctttta ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca
     3961 tcagaagtat cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt
     4021 acacacgtaa taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag
     4081 gctgctaagc caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa
     4141 ccagcaattg caccaccaat catggaagca actaatggtt ttttcagctt caaggttact
     4201 ccatagagtg aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc
     4261 tgcttcaact ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca
     4321 atattagaag ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc
     4381 atagtagcga ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa
     4441 ccacccatca aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt
     4501 gctataagat cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc
     4561 ataattaaaa ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga
     4621 tccgcaaagg gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat
     4681 cccgcataat ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa
     4741 aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg
     4801 ttgacatcaa actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct
     4861 gcatcagcaa tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc
     4921 aaagtcaaga cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc
     4981 ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct
     5041 gaagaggggc cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt
     5101 tggacttcat tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca
     5161 ccaagtgttt tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc
     5221 agtctggtgg cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat
     5281 acatttcttc ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac
     5341 caagtcaaac cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat
     5401 aatcagtaac atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag
     5461 cgcaatcaac agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac
     5521 agttagatca catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct
     5581 aatattaact ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt
     5641 gaaagcatca aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa
     5701 aattgatgaa tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca
     5761 atagtgatac ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg
     5821 agatataaag gtatcgatag aatgatttgg gctagtattc ttaaaaatta actagcaaat
     5881 gtttaaactg atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca
     5941 ataaaaatac caaacatatc cataataatt ttttcttttt catttctttt atattgttat
     6001 aacagtgaat aacataaaaa ttcccgacaa tcattgtaat atttttttgt cacctgtatt
     6061 agtatttaat ttataggaaa ctataaaaac tccctagtga atttacacct ttgggagttc
     6121 ctgtttggac ggtatgcata atacatttaa atcaatttaa cattaacacc tcagcatatg
     6181 gtatctccac atatatacgg tctttctttg gatagtactc aatatttatg ttgatagcac
     6241 ttccattgtc taatatagct ttagcccggt atgtgttatc tccattttca tgagtaatcg
     6301 tcacatcatc acatgatcga tttaagttgt actgttcttg taaaatctgt gttaccaatg
     6361 gttttgatga cttctctaat ccagatgtac ctgtcatttt tgaaaaaata atacctataa
     6421 tagccaagac aacaatacct ataatgacaa acttcttgtt catatttact tta
//
LOCUS       DACDSR010000117         6432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDSR010000117 DACDSR010000000
VERSION     DACDSR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.118"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1393
                     /locus_tag="IWK15_002517"
     CDS             <1..1393
                     /locus_tag="IWK15_002517"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9548055.1"
                     /translation="ELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQL
                     NDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHD
                     GDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMG
                     SGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKD
                     WPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHL
                     KETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLK
                     KNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKE
                     NDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEA
                     WLQGALEVFKHEQ"
     gene            1383..2600
                     /locus_tag="IWK15_002518"
     CDS             1383..2600
                     /locus_tag="IWK15_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9548056.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2613..5762
                     /locus_tag="IWK15_002519"
     CDS             2613..5762
                     /locus_tag="IWK15_002519"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9548057.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2247 a         1066 c         1320 g         1799 t
ORIGIN      
        1 tgaattactt tcagatgaag aatcaaaaga agatctgatt gccacaatcg ttgatatttt
       61 gggatatgca attgcaccag agtatctttt caacgtctta gcggatcaag caaaacaagc
      121 aacgttccaa ttgaacgatc taaacaaagc ctttgtgcaa ttggcatcta cctacaatca
      181 atttaatggg ttatttgatg atgtggatct ccaatcaaaa aaactaggaa cagatgagca
      241 acaacgaaat gtgacgatta ccgaagtcat caagaaattg aatgatgtag atgttttaga
      301 acacgatggg gacgtgatcg gggatgcgta tgagtttctg atcagtcaat ttgcttcaga
      361 agcagggaag aaagccggag aattttacac acctcatatg gtttctgata tgatggcgca
      421 aatcgtaacg ctcgatcaaa aggaacgacg attctttagt gtttttgatc caacgatggg
      481 ttcaggttca ttgatgttaa acgtacgtaa ctacttaaca cacccagata acgttaaata
      541 tcatgggcaa gaactaaata caacgacata caatctagca aaaatgaact tgattttaca
      601 tggtgtcgac gccgaagaaa tgaatctccg caatggggat acgttgaata aggactggcc
      661 aacagatgaa ccttacacat ttgatgcagt agtaatgaac ccaccttatt cggctaattg
      721 gtcagcagat acgactttct tagatgattc tcggtttaat cgttatggga aattagcgcc
      781 aaaatccaaa gcagattttg cgtttcttct tcatgggttc tatcatttga aggaaacagg
      841 taccatggcc atcgtcttac cacacggagt tttattccgt ggcgctgcag aaggggtcat
      901 ccgtcaaaaa ttactagaag atggcagtat ttatgctgtg atcggtatgc ctgcgaactt
      961 gttctttggg acatcgattc caacgacggt gattgtgttg aagaagaatc gtcaaactcg
     1021 agatgtatta ttcattgatg ctagtcgtga atttgttaaa gggaaaaatc aaaataaact
     1081 atcggaagaa aacattcaaa aaatcctcga aacctacgct gaaagaaaag atgtcgagaa
     1141 atacgctcat ttggcaacct ttgatgaaat caaagaaaat gactataact taaatatccc
     1201 acgctatgta gatactttcg aagaagaaga accaattgat atggttcatg tcggaaatga
     1261 catcaagaag atacgacaag aacagcaagt gctagaaaaa aaacttttag aagctatctc
     1321 ttccctgcaa acaacacctg aaaatgaagc gtggttacaa ggtgcgctgg aggtgttcaa
     1381 acatgagcaa tgatacacaa ccagaaatcc gtttcccagg atttacagat gattgggaac
     1441 agaggaagtt gggggacttc gttgtagatt atgttgaaaa gacaagtgta caaaatcaat
     1501 ttcctatgct aacttcatct caacaaaaag ggatagtttt gcaagaagac tattttgcaa
     1561 atcggcaagt aactacagaa aataatattg gttactttgt actacctaga ggatatttca
     1621 cgttccgtag tcgaagtgat aatgatgtgt ttgtgtttaa ccgtaatgac attattgata
     1681 gagggattat aagctatttt tatcctgtat ttactttaaa atctgctgat tccgacttct
     1741 tcttaagacg tattaacaat ggaatccaac gacaattatc tattcaagca gagggaacag
     1801 gtcaacatgt tctgtctcta aaaaagttta aaaatatagt agcaatgttt cctagtgagg
     1861 aagaacaaca aaaaatcggc accttcttca aacaacttga cgacacgatc gctcttcatc
     1921 agcgtaagtt agatttgctg aaggaaacga aaaaaggatt cttacaaaaa atgttccctg
     1981 aaaacggagc aaaagtgcca gaaatccggt ttcctggttt tacggaagat tgggaagagc
     2041 gtaagttggg agaattaggt aaaactcaat cgggaattgg atttcctgat gccgagcaag
     2101 gtggttctga aggtactcca ttttttaaag tttccgacat gaataacatt ggtaatgagt
     2161 atgaaatgag aaatgcaaat cattatgtat ctaatgagca aattgagcgt aaaaaatgga
     2221 agccaataaa agatgtgcca gcgattatat ttgctaaagt tggcgcagca ataatgctta
     2281 atagaaaacg tttggtcaca agccctttct taatagataa caataccatg gcttatttat
     2341 ttaataatac ttgggatatc tattttggta aaatattgtt tgaaactata aatttgccaa
     2401 gatactccca agtaggggca ttgccttctt ataacagttc tgatattgag aatatcagtg
     2461 ttaaggttcc tgtaaaggat gaacaacaaa aaatcggtac tttcttcaaa cagctagatg
     2521 atacgatcgc tcttcatcaa cgtaagttag atttactaaa agaaacgaaa aaaagcttct
     2581 tacaaaagat gtttgtctag gagggaagaa gaatgaaaat tagtcaccgt gaggaagcag
     2641 aagtcgaaga acagttgatt cgtgtacttg gagaagggca taatcagtgg acgtatcgtc
     2701 cggatctaaa atcagaagaa gacctttggg tcaatttacg ccaaaaaatt atctcaaata
     2761 atcaagcaga actgaatgat tttcctttga ccgataaaga atttgaaacg atcaaaacgg
     2821 aattactgct gcgaacgaaa accccatttg atgctgcgaa atggttaaaa ggtgaaaatg
     2881 ggatggctcg gatcacgatt gaacgggaag attcgcaatt gggttctgtc tcacttatcc
     2941 tttattccaa tcaagacatt ggtggaggaa tctctactta tgaagtggtc catcaaattg
     3001 caaaacgtgg aagtaacata gaagcacggg atcgacgttt tgacgtcacc ttattaatca
     3061 atggattacc gattgtccaa atcgagctga aacaagtaac tgcaaaagat ggtgtttatc
     3121 aggcatttaa ccaaatcaaa aaatatgcag aagaaggtat gtttcggaac aatatctttt
     3181 cgaccttaca gttgtttgtg gtttccaatg aacaaaccac tcggtatttt gcaaatgcct
     3241 taccgaaaga cttgcaccct aagtttttat ttagttggcg aacgaaagac aatgaaaaag
     3301 tagaaaactt atatgagttt tgtaaacaag tgttaaatat cccggatgct catcgtttga
     3361 ttgcggatta tacaatcgtc agtgaagatc aagacaataa aaccctcatg gtgttacatc
     3421 cttatcaagt acatgcgatt caagccttgt ttattgccgc aaataaacat caatcagggt
     3481 atgtgtggca tgcgactggt tcaggaaaga cgttgactag ttttgtatct actaagcttt
     3541 tagcacgaaa atcggggatc gatcgaacga tcatgttagt ggaccgcaaa gatttagata
     3601 atcaaaccac cacagagttt acaaaatttg cttcggaatt taatactggg atttcttccg
     3661 ggaacgccaa agcgaatagc ttgatcgttg gaacgggaag tgccaaagaa ttaagtgaaa
     3721 ccctgttggc agatgccaat gcaaatgttg taatcattac aacccgtcaa aaactagatg
     3781 cggcgttgaa atatgcaaag aaacaagaag agaaaaaagg aaccaatcgc ttccaaaaat
     3841 taatgggcca acatatcgtg tttgtagtag atgagtgtca tcgagcgtta agtgctgaaa
     3901 atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt acaggtacac
     3961 cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca acccatgatc
     4021 agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat ggctctgtct
     4081 taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag aataaaatct
     4141 atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag atcaatcgta
     4201 tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct gtttttgaag
     4261 cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat gcctatacaa
     4321 aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg agttccattg
     4381 acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct gattggttaa
     4441 caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat cctgattttc
     4501 cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt gcccaacaag
     4561 aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc tggtcattag
     4621 cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa agagcggaat
     4681 tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta ttaacaggtt
     4741 ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat gcaggattga
     4801 tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga ttgattgtga
     4861 cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa ctgtattcaa
     4921 aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa aaaagattca
     4981 aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat gaacatacgc
     5041 cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt gcttatgaat
     5101 cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact ttgaacaatc
     5161 aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt tcattaatag
     5221 aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat agtgaaaata
     5281 ccactaagtt gtatgatatt gattcaactt atattgatca attactagga acttatgctg
     5341 cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg aataaaacag
     5401 aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg actttagata
     5461 gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt gatgatttga
     5521 ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct tctaatctgc
     5581 aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac agtaaggatt
     5641 acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca caagaaatga
     5701 agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat gaattgaggt
     5761 aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg gaagtgtaga
     5821 aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt aagacagcta
     5881 ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt tatatgaccg
     5941 tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt tcttttgttt
     6001 ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag tgattttttg
     6061 ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag tttattgaga
     6121 aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat taaatggaat
     6181 catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac ctatgggact
     6241 gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg tcgtattgat
     6301 tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt ctctatatga
     6361 ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg gaataatcca
     6421 ataaaaaatg at
//
LOCUS       DACDSR010000118         6392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDSR010000118 DACDSR010000000
VERSION     DACDSR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.119"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IWK15_002520"
     CDS             <1..382
                     /locus_tag="IWK15_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP9548058.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="IWK15_002521"
     CDS             complement(776..1783)
                     /locus_tag="IWK15_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9548059.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="IWK15_002522"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="IWK15_002522"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9548060.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="IWK15_002523"
     CDS             3679..4002
                     /locus_tag="IWK15_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9548061.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="IWK15_002524"
     CDS             4013..5695
                     /locus_tag="IWK15_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548062.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6392
                     /locus_tag="IWK15_002525"
                     /pseudo
     CDS             5901..6392
                     /locus_tag="IWK15_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2034 a          981 c         1294 g         2083 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga ag
//
LOCUS       DACDSR010000119         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDSR010000119 DACDSR010000000
VERSION     DACDSR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.120"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWK15_002526"
     CDS             334..714
                     /locus_tag="IWK15_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9548063.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWK15_002527"
     CDS             696..1610
                     /locus_tag="IWK15_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9548064.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWK15_002528"
     CDS             1855..2544
                     /locus_tag="IWK15_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548065.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWK15_002529"
     CDS             complement(2665..2751)
                     /locus_tag="IWK15_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9548066.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWK15_002530"
     CDS             3427..4377
                     /locus_tag="IWK15_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9548067.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWK15_002531"
     CDS             4398..4931
                     /locus_tag="IWK15_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9548068.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWK15_002532"
     CDS             5567..6100
                     /locus_tag="IWK15_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548069.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDSR010000120         6288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDSR010000120 DACDSR010000000
VERSION     DACDSR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.121"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..205
                     /locus_tag="IWK15_002533"
                     /pseudo
     CDS             1..205
                     /locus_tag="IWK15_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801983.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            381..941
                     /locus_tag="IWK15_002534"
     CDS             381..941
                     /locus_tag="IWK15_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9548070.1"
                     /translation="MIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHL
                     TPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGK
                     PLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTL
                     NTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            938..2830
                     /locus_tag="IWK15_002535"
     CDS             938..2830
                     /locus_tag="IWK15_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9548071.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2837..3214
                     /locus_tag="IWK15_002536"
     CDS             2837..3214
                     /locus_tag="IWK15_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9548072.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3368..4150
                     /locus_tag="IWK15_002537"
     CDS             3368..4150
                     /locus_tag="IWK15_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9548073.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4276..5007)
                     /gene="erm(A)"
                     /locus_tag="IWK15_002538"
     CDS             complement(4276..5007)
                     /gene="erm(A)"
                     /locus_tag="IWK15_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9548074.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5065..5152)
                     /locus_tag="IWK15_002539"
                     /pseudo
     CDS             complement(5065..5152)
                     /locus_tag="IWK15_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            5516..6178
                     /locus_tag="IWK15_002540"
     CDS             5516..6178
                     /locus_tag="IWK15_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9548075.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         2244 a          866 c         1211 g         1967 t
ORIGIN      
        1 actgctatca tttaaagttg ctgtacgagt tcatggaaca gagaggtgtt attcttaatg
       61 atattaactt tgagttgtta gcagacttcg taggttggtt gagatatcct tcagcatcaa
      121 atgtaattga tcttcagtca aaaaaagcca taagagaaga aacgacagtg aatacaattt
      181 taaatgtagt tatgagtttt cttgattatt taagtagatt aggagaattt aaatcaattg
      241 atgtatttaa acaagcaaag ggaagaaatt tcaaaggatt tttacatcat gttaataagg
      301 gtagatacca aaagaatgtc ttaaagttaa gggttaaaaa gaaacagata agaacattga
      361 gatcagagga agttaagcaa attattgatg cttgtcatac gaaaagagat aaattaattt
      421 taatgcttat gtatgagggt ggtttaagaa tcggtgaagt gttatcgctt aggcttgaag
      481 atattgtcac ttgggacaat caaatccatt taacacctag agatgttaat gttaatgaag
      541 cttatattaa attaaggaag gaaagaacaa tacatgtgag taaagaactt atgtcacttt
      601 atacagatta cttgatatat gagtatagtg aggaattgga gcatgattat gtttttattt
      661 ccttaaaaga aggctatttt gggaaaccac taaagtacca aagtgttctt gatctagtta
      721 gaagaatagt taaaaggact ggaatagaat ttacatcaca tatgcttcgc cacactcacg
      781 caacgcagct aattagggaa ggatgggatg ttgcgttcgt tcaaaagaga ttaggtcacg
      841 cacatgttca gacaacgtta aatacctatg ttcatctttc agatcaggat atgaaaaatg
      901 agtttaataa atacctcgag agaaaggagc ataagaaatg aatgcttcta gtaaaaggaa
      961 aattattagt cagagtgaga ttagcaaaaa aatagctgta atgaatgaag aaatgcaggg
     1021 gttttgggct aataatagtt gggatataag aaaatgtcca catccttctg ccatagaatt
     1081 aagtaagaat cctgctttaa ggaatcgttg ggttcgtttt gaacgtgtta aaaatctgtg
     1141 gttaagaaca gaattgaaat atttttattt ttaccattta aacaatggaa tatggaatgc
     1201 aaaaactgtc tggattagaa aaggaacagt aattaataaa atgttagatt tcttagattt
     1261 aaagtatcct agcattactt caattactga agttcctatt gaaaaagcaa tgacggagta
     1321 tagaacttat ttgacaaaac ggggtgttag aattaccact actaattata agattactgc
     1381 taatcaagaa aaaacacctg taaaagctaa ttcctactat gttactaatc taaaacaatt
     1441 tatggagttt tatgagaact tttattttga tggagaggag tgggataaag acgtttggga
     1501 tagacgtaac ttacctttgc cagatgataa ggttaaccca acacaatatg aatatacaat
     1561 taactttaaa gggtttcgga atacatattt taaacaactt gtaaaaagat attgtaagtt
     1621 gagattgaac atggatagct tttcctatgt aagtgatatt gcccaaagac ttaaagagtt
     1681 ctttaatttt ctggacatga aatttaaaca agttcagaga gtacaccaat taacgagagt
     1741 ggaaattgaa gcatatttaa gtgaactaaa catgatggga ataaaaccta gtacaataac
     1801 tgggaggatc tctatattgg aaggactatt tagtaccctt cttaggctag aatgggatga
     1861 tgttccttcc aaaatattaa tttattctga ggactatccg aaaataccaa gagcaaaacc
     1921 acgctttata gatgaattcg tcctagagca attgaacagt catcttgata aattacccga
     1981 atatatagct acgatgacta tgattgttca agaatgtgga atgaggataa gtgaattgtg
     2041 caccttgaaa aaaggctgtc tattagagga caaagatgga gatttctttt taaagtatta
     2101 tcaatggaaa atgaaaaagg agcatatagt tccaatatct aaagaggtag ctttacttat
     2161 taaagttcgg gaagataaag tttcagagga atttccagat agtgaatacc tctttccaag
     2221 aaaagatgga tcgccattaa aacaagaaac atttagaggt gagttaaata aattagctta
     2281 tgagcaaaat atagtggata aatcaggtga gatttataga ttccatgccc atgcctttcg
     2341 ccatacagta ggaacaagaa tgattaacaa cgggatgccc cagcatattg tgcagaaatt
     2401 tttggggcat gaaagcccag aaatgacaag cagatacgct catatctttg atgaaactct
     2461 aaaaaatgaa tttactaaat ttcaggaaaa actggttacc aataatggag atgtgcttga
     2521 tctagatgaa gataatgaag tcgatgatgt agagcttcaa tggttcaaga aaaatataaa
     2581 tgcacaagtg cttccaaatg gttattgtag attgccagta gtagcaggtg gttgtccaca
     2641 tgcgaatgca tgcttagatt gcactcactt ctgtaccagt aagcaattct taccacagca
     2701 cgaagaacag ttagagcgta cagaagagtt attagccata gctaaggata aacaatggca
     2761 aagacaagta gagactaata gccgtgttaa agagcgttta gaacaaatca ttggaagttt
     2821 gacggggtaa ttatcaatgg ataaacaagt tagaaataca acagaaattg tacgtttggc
     2881 gaagcagaaa tcaaaaaaga caagggaaaa agtagacaaa gcgatttcta aattttcgat
     2941 tgaaggtaaa gttattaatt ttaattcaat agcaaaggaa gctaatgttt ctaaatcatg
     3001 gctttataag gaacacgata ttaggcaaag aatcgaatcc cttcgtgagc gtcaaataac
     3061 agcaaatgta gtctcaaaac ccaagaaaag ttctcgttcg gaggaaatcc ttattaaaac
     3121 cttaaaaaga agagtaatgg aattagaaaa agaaaataaa aaattacaga accaaattca
     3181 aaaattatat ggagatctgt ataataaaga ataattatta atctgtagac aaattgtgaa
     3241 aggatgtact taaacgctaa cggtcagctt tattgaacag tcatttaggt atatgtccaa
     3301 tctagggtaa gtaaattgag tatcaatata aactttatat gaacataatc agttacgagg
     3361 tgaaatcatg agcaatttga ttaacggaaa aataccaaat caagcgattc aaacattaaa
     3421 aatcgtaaaa gatttatttg gaagttcaat agttggagta tatctatttg gttcagcagt
     3481 aaatggtggt ttacgcatta acagcgatgt agatgttcta gtcgtcgtga atcatagttt
     3541 acctcaatta actcgaaaaa aactaacaga aagactaatg actatatcag gaaagattgg
     3601 aaatacggat tctgttagac cacttgaagt tacggttata aataggagtg aagttgtccc
     3661 ttggcaatat cctccaaaaa gagaatttat atacggtgag tggctcaggg gtgaatttga
     3721 gaatggacaa attcaggaac caagctatga tcctgatttg gctattgttt tagcacaagc
     3781 aagaaagaat agtatttctc tatttggtcc tgattcttca agtatacttg tctccgtacc
     3841 tttgacagat attcgaagag caattaagga ttctttgcca gaactaattg aggggataaa
     3901 aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg tggcaaacag tgactactgg
     3961 tgaaattacc tcgaaagatg tcgctgcaga atgggctata cctcttttac ctaaagagca
     4021 tgtaacttta ctggatatag ctagaaaagg ctatcgggga gagtgtgatg ataagtggga
     4081 aggactatat tcaaaggtga aagcactcgt taagtatatg aaaaattcta tagaaacttc
     4141 tctcaattag gctaatttta ttgcaataac aggtgcttac ttttaaaact actgatttat
     4201 tgataaatat tgaacaattt ttgggaagaa taaagcgtcc tcttgtgaaa ttagagaacg
     4261 ctttattact ttaatttagt gaaacaattt gtaactattg aaaatagaaa gaaattgttc
     4321 cttcgatagt ttattaatat tagtgacatt tgcatgcttc aaagcctgtc ggaattggtt
     4381 tttagtgaaa agaacacgat attcacggtt tacccactta taaacaaaag atcgatactt
     4441 tttgtagtcc ttctttgaaa tcaatggttg atgtcgttca agaacaatca atacagagtc
     4501 tacacttggc ttaggatgaa aatatagtgg tggtactttt ttgagcattt ttatatccat
     4561 ctccaccatt aatagtaaac ccaaagctcg ttgcagattt tgcaatcttt tcgcaaatcc
     4621 cttctcaacg ataagatagc tatatttagc ctgactttca aaggtaattc ttttgacaat
     4681 atccgtactg atgttataag gaatattacc atatatctta tagtttatat gttttgggaa
     4741 ggaaaatttt agaatatccg tttgaatcac ttttatattc tcagaggggt ttaccgcttc
     4801 tttagtcact tgacataagc ctccatcaat ttctatagca gtaactgatc gactcatttt
     4861 gactagctct ttggtaaaat gtccttttcc tgatccgatt tctattacgt tgtcttgttt
     4921 actgatattc gtgtgattca atatttcttt tacatgcttt ttagaagtaa taaaattttg
     4981 cgtgtcttta gggtttttct ggttcattat aaccttctcc ttactggtta taatgaactg
     5041 acattaagcg ttcattataa ccaattaatt ttgatttggt tgataacgaa ctttattaat
     5101 aacaaatata gaaaaagtac ccatactttt atctcctttc catttttttc attatgaatg
     5161 agataaagta atttctacta ctgcgatact ggtgcacata attgaaacct ccttatgtaa
     5221 ctgaatatga ttgtatcatc ttttcatttt agaataaacc tttatatgta attttgcact
     5281 tgacacttca gatggacgtt ttcttcaaaa taatatgttt tgaaacttga taagagaagc
     5341 cactgtcttt actaaaaagt aaaggctgcg ataaaggaca gtgactttaa taaagttgca
     5401 ctgttgtatt gtctttggat aaaattgtct aaagggaagt tttatcccca acgaaaaaac
     5461 atctaaagtg gtaagtaatg aaagaatgtc gataaagtag gaatggggga tctttttgaa
     5521 tacaatagaa tcacagtttg ataaggttgc agaagattac gatttcgtga atgagctttt
     5581 gaatgattat tcattctttg tgtctaatat gtctccaaag aaaggcagag cattagatat
     5641 cggatgtggc tcgggtttgt tagtggagaa attagcaagt tattatgatg aagtggtagg
     5701 gattgatatt tctaatcaaa tgctcgatct tgccaaatct aaacgtcaac taacaaatac
     5761 ggtctatctg aatatgaatg cagaacaact taattttaat gagaagtttg attttattgt
     5821 aagccgaaca acctttcatc atttggatga tatagccagc gtaatacagc aaatgaagga
     5881 actgttgaat gaggaaggaa gaatagttat tcttgataat gtatctgaag tcgagacacc
     5941 gcctacctat gtttataaat taggggcaat ccaagagttt ttaccgcatt gtttcaaatt
     6001 tggaataaaa aatgcgatta gaatttataa tcacaacaca tctaaatcat ggcttgagca
     6061 tttagcttca gataagtacc tatctgaaca aaattattac gatttatatg aaaagttgtt
     6121 gcctggatgc caattccata aaatgggttg ggcaatgggg gttgtctgga caaaataact
     6181 taaagctgtg gctttgaaaa gttgaatttg tgattgtttt cagagctatc gggtgcttta
     6241 gtataaataa caatgaataa aaaatcagcc tgtatcttat gttactat
//
LOCUS       DACDSR010000121         6217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDSR010000121 DACDSR010000000
VERSION     DACDSR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.122"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            71..1567
                     /gene="glpK"
                     /locus_tag="IWK15_002541"
     CDS             71..1567
                     /gene="glpK"
                     /locus_tag="IWK15_002541"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9548076.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            1581..3404
                     /gene="glpO"
                     /locus_tag="IWK15_002542"
     CDS             1581..3404
                     /gene="glpO"
                     /locus_tag="IWK15_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9548077.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            3405..4121
                     /locus_tag="IWK15_002543"
     CDS             3405..4121
                     /locus_tag="IWK15_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9548078.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            4427..>6217
                     /locus_tag="IWK15_002544"
     CDS             4427..>6217
                     /locus_tag="IWK15_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9548079.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLD"
BASE COUNT         2056 a         1068 c         1457 g         1636 t
ORIGIN      
        1 tcttttttta aaacagtagt atgataagag tagttaaaga aagcgtttgc atataatgga
       61 gggaatcaca atgacagaat caacgtatat catggcgatt gaccaaggta caactagctc
      121 acgagcaatt atctttgaca agaaaggaag acacatcggc agttctcaaa aagagttcac
      181 gcaatatttc ccgcaagaaa gttgggtgga acatgatgca aatgaaatct ggaattctgt
      241 acagtcagtt attgccggag cgttcatcga atcagggatc aaaccaaatc agattgctgg
      301 gattggcata acgaaccagc gtgaaacaac agtaatatgg gaaaaagata caggacgtcc
      361 aatctatcat gcagttgttt ggcaatccag acagtcagct ggaattgcag ataacttaaa
      421 aaatgaaggc tatcaagaat ttttccacga aaaaacaggg ttagtgatcg atgcctattt
      481 ctcagcaacg aagatacgct ggattcttga tcatgtagaa ggcgcgcaag aacgagctga
      541 aaaaggcgaa ttgatgtttg gaacgatcga ttcttggtta gtatggaaac tgaccgatgg
      601 acaagcacat gttactgatt actcgaatgc cagtcggacg atgttgttca atatccatca
      661 gctagattgg gatcaggaga ttttagattt actaaatatt cctcgagtga tgttaccaaa
      721 agtcacttct aactcagaag tttatggtta tacgcaaggt tatcattttt atggaagcga
      781 agcaccaatt tcagggatgg caggagacca gcaagccgca ttattcggac aaatggcatt
      841 cgaaccaggc atggtcaaga atacttatgg tacaggttca tttattgtca tgaataccgg
      901 tgaaaaaccg caattatcca aaaataattt attgacgaca attggttacg gcataaatgg
      961 aaaggtttac tatgctttag aaggaagtat ttttgtggct ggttcttcga ttcaatggct
     1021 gagagatgga cttcaaatgt tgcaaaaagc tagcgattca gaagacgcag cgaaacgctc
     1081 tacaagcgaa gatgaggttt atgttgttcc tgcattcgtt gggctaggtg ctccttattg
     1141 ggatcaggca gcacgagggg ctatgtttgg cttgacacgt ggtacgacaa aagaagatat
     1201 cattaaagct actttacagt cgattgctta tcaagtgaga gatatcatcg atacgatgca
     1261 agatgacact ggaatcaaga ttcctgtatt gaaagtagat ggcggtgcag caaacaatga
     1321 atatctgatg caattccaaa cagatatctt gaatgtaccg atccaacgag cagaaaactt
     1381 ggaaacaacg gcgctagggg cagcattttt agcaggatta gctgtcggtt attggaaaga
     1441 tacagacgag atccgcgaat tttatgaagc tggcaagcta tttgaagttc aaatggagga
     1501 agagcgccga gaaaaacttt acaacggttg gaaaaaagca gtaaaagcta cccaagcatt
     1561 tgaataagga ggaaatccat atgttttcaa acaagacaag acaagatagc attcaaaaaa
     1621 tgcagcaaga agaattggat ctgttgatca tcggtggcgg aatcactggt gccggtgtgg
     1681 cagtccagtc agcagcatca ggaatcaaaa caggattgat cgaaatgcag gattttgcag
     1741 aagggacttc ctctcgctcg accaaacttg ttcatggcgg tattcgttat ctgaagacat
     1801 ttgatgtgga agtagtagct gacacagttg gtgaacgtgc agtcgtacaa agtattgccc
     1861 cacacatccc aaaaccagat ccaatgcttt tgccaatcta tgagggcgaa ggagcaacaa
     1921 ccttcaatat gttctcggtc aaagtagcaa tggaccttta tgacaaactg gcaaatgtga
     1981 caggaaccaa atatgagaat tatacgctga caccagaaga agtattggaa agagaaccgt
     2041 ttttgaaaaa agaagggcta aaaggcgcgg gtgtctatct agatttccgc aacaatgatg
     2101 cccgtttagt gattgataat atcaaaaagg ctgccgaaga tggggcttat ctagtaagta
     2161 aaatgaaagc ggttggcttt ttatatgatg gcgatcaaat cgttggcgtc aaagctcgtg
     2221 acctgctgac agatgaagtg atcgaaatca aagcaaaatt agtgatcaat acgagtggtc
     2281 cttgggtaga taaagtacga aacttgaatt ttacgcgtcc agtctctcct aaaatgcgtc
     2341 caactaaagg gatccattta gttgtagatg cgaaaaaact gcctgtaccg caacccacat
     2401 acttcgatac aggaaagcaa gatgggcgga tggtttttgc tattccaaga gaaaacaaga
     2461 cttactttgg tacgacagat acggattacc aaggagattt tacggatccg aaagtcacac
     2521 aagaagatgt agattatcta ttggatgtga tcaatcatcg ctatccagaa gcaaatatca
     2581 cattgaatga tatcgaagca agctgggcag gacttcgtcc actgttgatt ggtaattctg
     2641 gttctgatta caatggtgga gataatggat cgatttcaga caagagcttc aataaagttg
     2701 tcgatacagt aagtgaatat aaggaaaaca aagtttctcg tgctgaagta gaagacgtgt
     2761 tgaaccattt ggaaaacagc cgtgatgaaa aagcaccttc tacgatttcc agaggtagtt
     2821 ctttagaaag agaaccagat ggtttgctga ctttatctgg tgggaaaatc accgattacc
     2881 gtaagatggc agaaggagct ttgcgattga tacgccaatt attaaaagaa gaatacggaa
     2941 tagagacgaa agaaatcgac tctaaaaaat atcagatttc aggtggaaac ttcgatccaa
     3001 ctaaattaga agaaacagtg acagaattag caaaagaagg agtagcagcc ggtttagagg
     3061 aagaagatgc tacttatatc gctgattttt acgggactaa tgcccgacgt atctttgaat
     3121 tagcaaaaga aatggcacct tatcctggtt tgagtctcgc tgagtcagct cggttacgtt
     3181 atggtttaga agaagaaatg gttttggccc caggcgatta tctcattcgt cgtacaaatc
     3241 atctgttgtt tgaacgagat cagcttgatg agatcaagca acctgtgatc gatgcaattg
     3301 ctggatattt tgaatggaca gaagaggaga aggcgcaaca gactaaacgt ttagaagcat
     3361 tgattgcaga atcagatctg cgggaactaa agggggagaa ataaatgaac agtgatatga
     3421 ctcaaatcat gggagagttt attgggacac tgatcctagt tttgcttgga gatggtgtgt
     3481 gtgcggctgt gaatttaaac aaaagtaaag cacaggcttc tggctggatt gtcatcgcat
     3541 ttggttgggg actcgctgta acaatggctg tctatatttc tggattcatg ggcccagctc
     3601 atttgaatcc tgcagtctct ctagctatgg cgatgacagg ggcaattagt tggaatcttg
     3661 tcgttccttt catcattgcg caagttttag gagcttttgc aggggccatt cttgtttggc
     3721 tttcttattt gccacattgg aatgcaacga aagatgaaag cgcgatttta ggaacgtttg
     3781 caacaggtcc tgctatacgg aattatccag caaatgtgat cacagaatta attggtacat
     3841 ttgtattagt tcttgggttg ctagcttttg gacaaaacga atttgcacct ggaacgaacg
     3901 tattcgctgt aggtggattg atcttagcca tcggtctttc attaggaggg ccaacaggat
     3961 atgcaatcaa ccctgctcgg gactttggtc ctcgattagc acatgcggta ttgccaatag
     4021 ctaataaagg aacttcagat tgggcatatt catgggtacc aatcgcaggt ccaatgatcg
     4081 gagcaattat cgcggtaggt gtctattcat taatggtgta aatctataaa acatactaaa
     4141 gtttgacaac caaaatgtgt cttgctatag ttaagtggaa aataaataag acctagaaga
     4201 atggggtatg ttttgtgagt gtagctggta tctaatcagt aagtcgaata gccaataaac
     4261 aaagtttatt tggtgtgctt atgaaacggt acctgtcagg aaaacaatat ctttgtattc
     4321 taataaggat aggacatgac ttttttaagc tgtgttctat ccttttattt atctctaggc
     4381 ccttatttat tttgggacag gtatctcttc aagaaaagga gagaatatgg acaacgaaat
     4441 gattagtcaa ttacaatttg atcggatcaa aaaggaaatc caagcaagag caattggaaa
     4501 ctatagcaaa aaacgaatca gcgatatgac ggtctcgaca aacctacaaa ctgtcttgga
     4561 tcgtcaagaa gaaacaagag aagcacgctt gatcttagaa agcagtcagc atgttccatt
     4621 tatggggttg ccgaggatcg atgcactgac agaacaagtc aaaaaagggt tgattctgca
     4681 gccaaccgat ttgatcgaat atgcggattt tcttagaagc agtcggatga tcacaaagtt
     4741 ttttgataag aatcaatacc agacaccctt gttgtttgct tatagcaaac atcttcctga
     4801 tttgataaat gtagaagagt tgattgacca gaaaataaaa aataataaag tcagtgacga
     4861 cgcgtcacgt aatttgcgta aagtacgcaa acaattacaa ctaatcgaaa aagaaatcca
     4921 gtcaaaatta ctgaaattct tgcgtcatcc gaaaaacaaa gaaatgatcc aagaagcaat
     4981 gattgttcaa aaaggcgaat actatacgat tcctatcaaa gccagctata aaaataaaat
     5041 agatggaacg attatcgatg aatcaaataa aagaacgacg gtttttattg aaccaactgt
     5101 gatttctaaa ttgaatgaac actatcagtt gttaaaagct gaagaaatca gtgaagaata
     5161 tcaagtcctg gcggcattga ctggtgctat cgcagaaaac gaagaagtga tcgatttatt
     5221 gatcgaaacg atcacggtgt tagatatcat ctttgctcgg gcaaaattta gccgagaaat
     5281 caatggaagc acacctagaa tcaataaatc ggaacatatc attatcaagc aaggcagaca
     5341 tccattttta cctgagcatg cggtaccatt ggatgttgaa atcggtaaag actatcgagg
     5401 cttgattatc accggagcaa atgccggagg caaaacagtc gtgttgaaga cagtcggttt
     5461 attgactttg atggcgatgt tcggtatgca agttccggca aaagaaggga cagagcttgc
     5521 tgtctttgat gagattttcg tcgatgtcgg tgaccaccaa aatttagaaa atgcgttgag
     5581 tacattttct ggtcatatgc aaaacatcgc tgctattttg aaaaagataa aaaggaatac
     5641 gttggtactt ttagatgaga ttggcagtgg aacagagcca aatgaagggg ctgcactagc
     5701 gatcgcaatc atggaatcta tgtatgagca aggggcattg atcattgcca cgacccatta
     5761 tggagaaatc aagaaattcg ccggagatca tgaagatttt gtgccggctg ctatggcttt
     5821 cgatcgggaa gcacttcgtc ctaaatatca gttgcgggtt ggagaaacag gagaaagcca
     5881 agctttgtgg attgctcata aaatggatat gtcgatgaaa ttgatccatc aagcagagcg
     5941 ttacttggcg gaaaaagcct atcggacaca gaaaaaagaa tttcttccta agacagcagt
     6001 tacaaatagg aaggagaata aaaaagaacg ccagctcttt gcaaaaggtg atcggatatt
     6061 tgtcaatgaa taccaaaaag aagcgttagt ttatgaagat ataggtgaag atacgattga
     6121 tgtatattta gacaaaaaaa taatccacgt gcctcgtcag cgtgttcgtc tggtgcgttc
     6181 agcagaagat ctgtacccga caggttacga tttagac
//
LOCUS       DACDSR010000122         6089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDSR010000122 DACDSR010000000
VERSION     DACDSR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.123"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            112..801
                     /locus_tag="IWK15_002545"
     CDS             112..801
                     /locus_tag="IWK15_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548080.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            926..3277
                     /locus_tag="IWK15_002546"
     CDS             926..3277
                     /locus_tag="IWK15_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9548081.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3357..3752
                     /locus_tag="IWK15_002547"
     CDS             3357..3752
                     /locus_tag="IWK15_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548082.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4077..4694
                     /locus_tag="IWK15_002548"
     CDS             4077..4694
                     /locus_tag="IWK15_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9548083.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            4735..5034
                     /locus_tag="IWK15_002549"
     CDS             4735..5034
                     /locus_tag="IWK15_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548084.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5068..5235
                     /locus_tag="IWK15_002550"
     CDS             5068..5235
                     /locus_tag="IWK15_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9548085.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5280..5885)
                     /locus_tag="IWK15_002551"
     CDS             complement(5280..5885)
                     /locus_tag="IWK15_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9548086.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2089 a          854 c         1203 g         1943 t
ORIGIN      
        1 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
       61 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      121 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      181 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      241 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      301 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      361 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
      421 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
      481 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
      541 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
      601 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
      661 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
      721 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
      781 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
      841 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
      901 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
      961 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1021 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1081 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1141 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1201 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1261 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1321 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1381 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     1441 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     1501 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     1561 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     1621 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     1681 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     1741 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     1801 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     1861 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     1921 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     1981 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2041 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2101 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2161 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2221 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2281 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2341 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     2401 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     2461 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     2521 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     2581 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     2641 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     2701 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     2761 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     2821 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     2881 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     2941 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3001 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3061 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3121 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3181 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3241 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3301 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3361 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     3421 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     3481 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     3541 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     3601 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     3661 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     3721 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     3781 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     3841 ataactagtt tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga
     3901 ataaatataa tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg
     3961 ccttgatttt ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc
     4021 tgatttaatc tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga
     4081 gaaaattttt ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt
     4141 ccgcgaagga attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag
     4201 attttaaacg ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct
     4261 atctacaatc attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag
     4321 aattaactaa ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg
     4381 ctagttacaa gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac
     4441 tcagctatat ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag
     4501 aagctgcgaa acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat
     4561 ttcctgcgat ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc
     4621 aattgggcat gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc
     4681 accacttttc ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac
     4741 ttaacgaatc aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc
     4801 atcggtttgg ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat
     4861 ttcctggttt gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag
     4921 gaacaaaccg cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc
     4981 gaattttatg cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta
     5041 gtaaaattta caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt
     5101 gaaataacta gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta
     5161 gcaaaaagtg tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg
     5221 gtacatggaa catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc
     5281 tacaaatcat tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt
     5341 gcaatctctg caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag
     5401 cgtttttgcg gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg
     5461 gctttttgag ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt
     5521 tgtgcaatat aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca
     5581 tttccagtat taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt
     5641 ttcataaaag caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat
     5701 gtaacgatga cgcaatcacc ttcacggata taatttagta atttttgaaa ctcaggacgc
     5761 tcagtatctt taccactggc tttttcttgg aataatttat caatgttaag tccttccatc
     5821 attgccaatt gtcgatccaa attttgatct tgtgaagata ctcgaatata gccaattttt
     5881 cccataaaaa actccttttt aaaagtgata taatttacat gtaaaattta cttgtagttt
     5941 tactgctatc atagcatttt taaaaataaa ttttatgtag acttaaaatt tacttcatgg
     6001 agggtttaaa tggatgaaat caaaagtaac aaaaaagcat ttggcattta tttaggagaa
     6061 cctagtacag aacaattaaa ccaattttt
//
LOCUS       DACDSR010000123         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDSR010000123 DACDSR010000000
VERSION     DACDSR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.124"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK15_002552"
     CDS             <1..395
                     /locus_tag="IWK15_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548087.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWK15_002553"
     CDS             398..1228
                     /locus_tag="IWK15_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9548088.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWK15_002554"
     CDS             1244..2095
                     /locus_tag="IWK15_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9548089.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWK15_002555"
     CDS             2092..2451
                     /locus_tag="IWK15_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9548090.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWK15_002556"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWK15_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWK15_002557"
     CDS             2624..2929
                     /locus_tag="IWK15_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548091.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWK15_002558"
     CDS             2929..3240
                     /locus_tag="IWK15_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548092.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWK15_002559"
     CDS             3407..3823
                     /locus_tag="IWK15_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9548093.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWK15_002560"
     CDS             3823..4491
                     /locus_tag="IWK15_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9548094.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWK15_002561"
     CDS             4488..4865
                     /locus_tag="IWK15_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548095.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWK15_002562"
     CDS             4862..5161
                     /locus_tag="IWK15_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548096.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWK15_002563"
     CDS             5403..5819
                     /locus_tag="IWK15_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9548097.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDSR010000124         5972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDSR010000124 DACDSR010000000
VERSION     DACDSR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.126"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            26..415
                     /locus_tag="IWK15_002564"
     CDS             26..415
                     /locus_tag="IWK15_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP9548098.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            543..1226
                     /locus_tag="IWK15_002565"
     CDS             543..1226
                     /locus_tag="IWK15_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9548099.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1535..1608
                     /locus_tag="IWK15_002566"
     tRNA            1535..1608
                     /locus_tag="IWK15_002566"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1569..1571,aa:Arg,seq:tct)
     gene            1767..2126
                     /locus_tag="IWK15_002567"
     CDS             1767..2126
                     /locus_tag="IWK15_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9548100.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2249..3880
                     /locus_tag="IWK15_002568"
     CDS             2249..3880
                     /locus_tag="IWK15_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9548101.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3942..4583)
                     /locus_tag="IWK15_002569"
     CDS             complement(3942..4583)
                     /locus_tag="IWK15_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9548102.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4865..5173
                     /gene="rplU"
                     /locus_tag="IWK15_002570"
     CDS             4865..5173
                     /gene="rplU"
                     /locus_tag="IWK15_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9548103.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5200..5544
                     /locus_tag="IWK15_002571"
     CDS             5200..5544
                     /locus_tag="IWK15_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9548104.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5557..5850
                     /gene="rpmA"
                     /locus_tag="IWK15_002572"
     CDS             5557..5850
                     /gene="rpmA"
                     /locus_tag="IWK15_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9548105.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1855 a         1002 c         1280 g         1835 t
ORIGIN      
        1 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
       61 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      121 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      181 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      241 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      301 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      361 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      421 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      481 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      541 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      601 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      661 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      721 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      781 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      841 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
      901 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
      961 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1021 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1081 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1141 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1201 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1261 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1321 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1381 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1441 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1501 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1561 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1621 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1681 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1741 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1801 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1861 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     1921 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     1981 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2041 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2101 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2161 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2221 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2281 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2341 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2401 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2461 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2521 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2581 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2641 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2701 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2761 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2821 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     2881 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     2941 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3001 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3061 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3121 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3181 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3241 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3301 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3361 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3421 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3481 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3541 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3601 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3661 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3721 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3781 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3841 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     3901 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     3961 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4021 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4081 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4141 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4201 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4261 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4321 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4381 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4441 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4501 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4561 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4621 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4681 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4741 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4801 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4861 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     4921 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     4981 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5041 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5101 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5161 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5221 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5281 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5341 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5401 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5461 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5521 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5581 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5641 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5701 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5761 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5821 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     5881 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     5941 tttactttgt ctttttacac ttaatatttt tt
//
LOCUS       DACDSR010000125         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDSR010000125 DACDSR010000000
VERSION     DACDSR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.127"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IWK15_002573"
     CDS             complement(<1..985)
                     /locus_tag="IWK15_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9548106.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IWK15_002574"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IWK15_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9548107.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IWK15_002575"
     CDS             1810..2964
                     /locus_tag="IWK15_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9548108.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IWK15_002576"
     CDS             3179..4147
                     /locus_tag="IWK15_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9548109.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IWK15_002577"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IWK15_002577"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9548110.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IWK15_002578"
     CDS             5177..5785
                     /locus_tag="IWK15_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9548111.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACDSR010000126         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDSR010000126 DACDSR010000000
VERSION     DACDSR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.128"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWK15_002579"
     CDS             450..1604
                     /locus_tag="IWK15_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9548112.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWK15_002580"
     CDS             1598..2020
                     /locus_tag="IWK15_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548113.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWK15_002581"
     CDS             3143..4075
                     /locus_tag="IWK15_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9548114.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWK15_002582"
     CDS             4092..4607
                     /locus_tag="IWK15_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9548115.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWK15_002583"
     CDS             4903..5184
                     /locus_tag="IWK15_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548116.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWK15_002584"
     CDS             5186..5602
                     /locus_tag="IWK15_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548117.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDSR010000127         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDSR010000127 DACDSR010000000
VERSION     DACDSR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.129"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWK15_002585"
     CDS             complement(99..1229)
                     /locus_tag="IWK15_002585"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9548118.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWK15_002586"
     CDS             complement(1877..3079)
                     /locus_tag="IWK15_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9548119.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWK15_002587"
     CDS             complement(3139..3342)
                     /locus_tag="IWK15_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9548120.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWK15_002588"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWK15_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWK15_002589"
     CDS             4879..5691
                     /locus_tag="IWK15_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548121.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACDSR010000128         5613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDSR010000128 DACDSR010000000
VERSION     DACDSR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.130"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWK15_002590"
     CDS             complement(59..2008)
                     /locus_tag="IWK15_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9548122.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWK15_002591"
     CDS             complement(2050..3108)
                     /locus_tag="IWK15_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9548123.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWK15_002592"
     CDS             complement(3227..3682)
                     /locus_tag="IWK15_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548124.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWK15_002593"
     CDS             complement(4266..5069)
                     /locus_tag="IWK15_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9548125.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2075 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat att
//
LOCUS       DACDSR010000129         5612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDSR010000129 DACDSR010000000
VERSION     DACDSR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.131"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWK15_002594"
     CDS             66..767
                     /locus_tag="IWK15_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9548126.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWK15_002595"
     CDS             764..1885
                     /locus_tag="IWK15_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9548127.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWK15_002596"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWK15_002596"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9548128.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWK15_002597"
     CDS             3264..4172
                     /locus_tag="IWK15_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9548129.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWK15_002598"
     CDS             4207..4479
                     /locus_tag="IWK15_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9548130.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWK15_002599"
     CDS             4490..5536
                     /locus_tag="IWK15_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9548131.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1821 a          972 c         1219 g         1600 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gc
//
LOCUS       DACDSR010000130         5192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDSR010000130 DACDSR010000000
VERSION     DACDSR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.132"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..1073
                     /locus_tag="IWK15_002600"
     CDS             99..1073
                     /locus_tag="IWK15_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9548132.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1055..1516
                     /locus_tag="IWK15_002601"
     CDS             1055..1516
                     /locus_tag="IWK15_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9548133.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1566..2030
                     /locus_tag="IWK15_002602"
     CDS             1566..2030
                     /locus_tag="IWK15_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9548134.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2044..2793
                     /locus_tag="IWK15_002603"
     CDS             2044..2793
                     /locus_tag="IWK15_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9548135.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2793..3623
                     /locus_tag="IWK15_002604"
     CDS             2793..3623
                     /locus_tag="IWK15_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9548136.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3623..4270
                     /locus_tag="IWK15_002605"
     CDS             3623..4270
                     /locus_tag="IWK15_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9548137.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4368..5144)
                     /locus_tag="IWK15_002606"
     CDS             complement(4368..5144)
                     /locus_tag="IWK15_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9548138.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT         1766 a          706 c          940 g         1780 t
ORIGIN      
        1 tttgaaaaat agtattgcaa aataaagcaa tttcatagat aataggtatt gtaaagttat
       61 atgactttat aatacttatt agagtgaggg ataaagaaat gagacaacta cacaaagaac
      121 agattgaaac agcaatggca gcgttaaagg aaaagacaat tgatcatgta tattttacag
      181 catgtggggg ttcacaagcc gttttaatgg ctggtcaata tctatttgat aaagaattaa
      241 ctgtaccttg tcatgtttat actgctaacg aatttaacta tgctatacca aaaggattat
      301 ctgaaaattc tttagttatc acttgttccc attctggcac aacaccagaa actgtagaag
      361 caacaaaaaa agcaagagaa tttggagcaa tgactatttg tttatctaat gaagaaggat
      421 ctcctctatg ggaagcagct gaatatcctg ttcactatga ttggggtaaa gatgttgatg
      481 ctagtgattt aaataaagga attctttatg gtttattatt taatatttta cttgttttag
      541 atggctctaa aaaatgggaa acttgcttga atgaatttga taagttaaat gatcttgtta
      601 ttgctgaaaa gagcaaattt gaaataactg ctaaagaatg gggtaaaaac caaaaacgag
      661 ataaaattat ttacacaatt ggtagcggta tcaattacgg agaaatttac tccacagcag
      721 cttgctggtt catggaaatg caatggatta attctaatgc tattcattct ggggaatttt
      781 tccatggtcc ttttgaaatc actgattatg atgtaccatt catcttagtg aaaagtattg
      841 gagatacaag atttttagat caacgggtag aagacttcac taaaaaattc agtaataaat
      901 taatggtttt agaccaagct gattttgact tagaatctat agatagtgta gcgaaagaat
      961 atattgcagc tgtacttagt ggcgttgtta ttcgccaaat ggtagaagcg attgcttttg
     1021 aaagaggcca ttccttagat gttagacgct atatgtggca aatgaaatat taattaatag
     1081 gaggttgcaa aagatggcaa atcaattatt tcacattgtg attacaacac attctaccct
     1141 ttgtgagggt tatttaaagg caacaaaatt aattttaatg aatgacgcca caggtattag
     1201 tgctgttcca tttaaagaaa atactagtcc agatgatttt gaggagagta tgaaagaaat
     1261 aattagtcag catcatttgc aacctctaat tattttaacg gatttaattg gaggaacacc
     1321 taataatgtt tctattaaac agttgaacaa caatagaatt caaattattg ctgggattaa
     1381 tttacctctt ttactggagt tattaatggc ccagtcagct ggtaaatcta tagaagaaat
     1441 agattttaag gaattactga ataactctca aaaaagtatg gttcatatta attcgctttt
     1501 aaaagatgtc agttaaggtg gcaatttcta ttctataaat tttaaaaatt gaaggaggaa
     1561 atagaatgat taaattagtg cgtgtagacc acagattact tcatggacaa gtaatatttt
     1621 cttggactaa acagtatgat attaaccata ttattgtggc tgataacaaa gttccaggag
     1681 atccaatgtc tgtgatggct ttgacaattg ctaaacctgc tgattgcaaa ttagacattg
     1741 ttaaaattag tgatgttaaa gggattatgg aaacatcccc aaatgataat tacatgattt
     1801 taattaaagg acctaaggaa gcttttgaat tagttcaaaa tttaccagaa attaaagaaa
     1861 ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca atatggaaag gcagtctttt
     1921 taactcctga agaattagaa gccacaaaaa aaattcttga tcaagaagtg aaaatatttg
     1981 tgcaacaagt cccatcttca agtattgaaa atgtagattt tagtaagtaa ggggggaaaa
     2041 taaatgttat taaaagccat tttattaggt ttagttggtg tggtagctgt tattgactcc
     2101 aggttaattg gtcgacaaaa tattggtaga ccactaattt tgagtacact tgccggactt
     2161 gtgctaggtg acttgcatac agggattgtt cttggggcat ctttagaatt aatttctatg
     2221 ggattcgtct caattggggc ggctggacct cctaatatgc aacttggtag tattattgcc
     2281 actgcttttg caattttaac taatagtagt acagaaacag ccttaacaat agctatccca
     2341 gtggctgtag ctggtgaatt tatgagtatt atcatgcgca tgataattgc tcaatttgga
     2401 catgctgcag ataaagccat tgaaaataat aaatttagaa aagctcaaat tattcatatt
     2461 tattggtcat ttatttttaa tgcttttgtt tactttattc caatcttttt aacagtatat
     2521 tttggtgctg acgttgtggc agatttagta gataaaattc cagaaatgat tacagacgcc
     2581 ttgactgtct ctggaaatat gctttccgca ttagggtttg caatgctatt aagcacaatg
     2641 ttgagtaaaa aattatatcc ttactttatt tttggattct ttattgttgc ctattcaggt
     2701 ctttcactaa ttggcgtgac aatttttgca gctctgattg cctttgtaat ggatcaagtt
     2761 aaatatggaa aaagagaaga ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt
     2821 taatcacaaa aaaagatctt aataagatgt gtcttcgttc atggacaatc atgtgttcgt
     2881 ggaattatga aagacaaatg catatgggat ttatgtatgg gatggcgcca attttaaata
     2941 aagtttataa aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat atggcatttt
     3001 ttaactgtac gcctcaaatg acttcattta ttatgggact ttgtgcttct atggaagaac
     3061 aaaatgctca agcagaagaa ggaaacttta atactgaatc tatctccatg attaaaacca
     3121 gtttaatggg tccttttgct ggaattggtg attcattttt ccaaggtact ttaagaatta
     3181 ttacatttgg gattggattg tcttttgcta agcaaggtag tattttagga ccgattttag
     3241 ctgttggcct ttttgctata ccttctattt tagctgtttt ttacggcggt aaattaggat
     3301 atgagtctgg caataaatac cttactaaat tgtatcaaga aggtcttatg gatcgagtaa
     3361 tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg aatggtggct agcatggtgc
     3421 caattgtcac ccctttaaaa ttcactacag ggggaacaga acttgtttta caagacttac
     3481 ttgattccat tattcctgga ttattaccac ttgtatgtac tttgggaata tataaattga
     3541 ttaagaaaaa tgttagtaca aatatgctct tattaggttg tattttggca gggtttgttt
     3601 taagtataat aggtatctta taatgattaa ggctctaatt tttgatatgg acggagtttt
     3661 aattgatagc gagattatgt atcaaaagtg ggtatttaat tttttaaaac aagaaaattt
     3721 aagctatcct ttgtattctt atcaaaaaaa gattggaact acagcaagta tttttgatga
     3781 tatagaaaag tacaatgagg gatgtaacag tttagaattg aagggaaaat ttactaaata
     3841 ttggaaatcg caaaagattg attacttgga tatttttcct aatatactaa ttgaacagct
     3901 tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct tcttccagtc caatggatac
     3961 tataaatgaa gttgttacca cttgtaaaat agagtcatat tttgattgtt taattagcgg
     4021 aagagatttg ccagaaagta aacctaatcc tactatattc cttaaagctt cagaacagct
     4081 cagaattcca gttgaagaat gtctggtgat agaagattct tataatggta taaaagctgg
     4141 taaaagagca aatatgaagg ttcttgcaat taaagataaa cgtttttctc aagatgtaag
     4201 tctagcagat gatgttattt acgatattaa atatctgcga caagaagtac aggtgaaatt
     4261 tggaatataa ataagttttt tgatgtattt agagaatatt agtaaaaata aaaatcatgc
     4321 ccaaatctaa tcaaaattga ttgctttggg tatgatttct tttaatatca atctattact
     4381 ttaggatctg aatcattttt atgcaccgta tctaaagtga tattttctgt gacttcaata
     4441 acataactaa ttctatcaca acgaactgtg gattgagaat aatgaaatgg ttgattattt
     4501 ttatctttgc caaccttttc taaaataaat agcggatctc ctactataca ttgcaataag
     4561 tcggaaagct caggtgttgc agataaaccg tttatttcca taatagagct cgtagctttg
     4621 acatcatagt catcttctag aagtttatat aatgaagtat attggctacc tttttcaaat
     4681 aaagttggaa atttttcttc tgatgcgaaa aaattatcaa ttcccatcgg ggcaccgtct
     4741 tcccacatta aacgtttaat attgtaaact ctatcagtta cattgatttg taatttatta
     4801 gctaaaaact catcagcctt tataatttta gaatgtaaaa cagtatatct aattttatgt
     4861 cctcgggtag aaagagaatc gctaaatcca tgaactccag ctaattttaa actttctttt
     4921 ttttgagtta caaaggtccc tttccccctt aatcgttcaa ctgttccact ttctgctaaa
     4981 agtttaaaag cttttcgaat ggtaatctta cttactccat aaattttaca taattcttct
     5041 tctgtaggta attgctctcc tggaagatat tttttttcac gaatatcgtt acgtatagaa
     5101 gcttctactt gtaaatataa tggtctatta tcttgaaaat tcatagtaca ctccctaaaa
     5161 agttatactt cattatatca caatttatta aa
//
LOCUS       DACDSR010000131         5162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDSR010000131 DACDSR010000000
VERSION     DACDSR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.133"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1165..1830)
                     /locus_tag="IWK15_002607"
     CDS             complement(1165..1830)
                     /locus_tag="IWK15_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548139.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1956..3992
                     /locus_tag="IWK15_002608"
     CDS             1956..3992
                     /locus_tag="IWK15_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9548140.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3996..4634
                     /locus_tag="IWK15_002609"
     CDS             3996..4634
                     /locus_tag="IWK15_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548141.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4662..5090
                     /locus_tag="IWK15_002610"
     CDS             4662..5090
                     /locus_tag="IWK15_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548142.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1705 a          782 c          948 g         1727 t
ORIGIN      
        1 gctaattttt tgattaaatg gtaagttttt ccttgtttgt tttagtttaa actagtgtgt
       61 aatttaaaaa tctatatgga agggtctgtg gggtaatact taaataaagg ctaggaacct
      121 gataacatca aggttcctag cctttttata ataaaatgaa gcgaaaataa tttcttttaa
      181 agagccgaga ttatcagcta tataagagtg aatttgatgc aaaaaacaga gccgaaacag
      241 tcttggtgtt cgtagctctg cttatatgtt tcgaatttaa gacaaagagt tggcaaaaga
      301 tgttgataag gaagataaga taactgtgtt atataaacta ggtaactaac tgtaacaagc
      361 taattttaag agattcttat tagaagacgt ttaggaatat aactaagaaa atgattatac
      421 aggtaattgt catttcaata atgttttcta attttcgcgt ctttgtttta agaaaaaggc
      481 cagaatcagt tggaaaaaag ttcgtacgat agtgaagagg ggcaaaattt cctactaata
      541 ttcccactaa caagagataa aacggccagg ttggtgtgat tcctctataa aaaaatgccc
      601 atgctaccga aatcccatag attaacaact gaacaatttt tacaatgaaa aaattgttct
      661 gccattactc aaagtctctt cctccttgta tcgtttattt gtttcatttt atcattatga
      721 taggcgcttg aacaatccat tttgtatttt attgagtact cttggataaa ctaccagcga
      781 ttatcatatt tatttatgtt actgtaagcc atcatataaa agtaaatgag cagatcatag
      841 aaatataaaa aagaactaaa attgatctaa attatgtgca gcttgactac gtaccaatct
      901 atattactta ctagattggt cgttaatcta ttcggatcga atgagcaaaa tatttttgtg
      961 aatgtccatc cttgattatg attccaaaaa gaatcattgg gaggctgtgg cgtaaatatt
     1021 atactataag ccagaaatac ctatcattct gaactttttt atttaggaac tttcatctca
     1081 aggctttttc aattaatttg tacccctgtt ttttggataa gatcatgtgc attgattttt
     1141 tctatctcta cgttcatctc ctacttaatg cctatctggc tcctgagggc ttctttttaa
     1201 ggcgtcgtca tagtcttggc tgaggatttc ttggtaatct gagggaatgg cttcccaagt
     1261 gagcaggcga acgttcatga cgtgttttcc tttgtgtttg attacagcat ggtttttctc
     1321 tttttctacc tctccagcgt gaaaagacag ccatgtacca tggccaaaga tatcattaca
     1381 tacccaacgt tcgatggaat tgtccacgta tcctacaggg agtgtggtat agccgatttg
     1441 tggtttcaaa aaaggattga atcgatcgat cgtttcattt ttgtattgat aattgataag
     1501 aaaaagatag accaaacaaa agacgagcag aaagaaagat agtgtctttt tgagtgagaa
     1561 gggttgtttt gatgaataag atttcttgtt aacaagaggt tcatctggca atgttggttc
     1621 tattgattcg gtagctgatt caatagaagg aaaatcaatc ggcagaccta aagtatgatg
     1681 taaaagtgac agcatttcaa ggtctggcat tgtacgagaa ttttcccaac tagaaatcgt
     1741 tttatcggaa acaccaattt tttcgcctaa ttctttttgc gtaagcttac aatttctgcg
     1801 gtaagttcgt attttttctc cggtgttcat cttcttctcc tttcgattct acagtatata
     1861 gatttattct acatgaccta tacacttgat agatacgttt ttttgatgaa aaacggtatg
     1921 attttatcaa agaatcattt aagtaaggga gagtggtgat aatcaaaaaa aagttcacag
     1981 ttttactcac aatcatagtg accggtatca tgagtggttt tgccttggtt agtataaata
     2041 aacaggtact ctatcggaca aatgaatttt tccatcaaac agatacttat actccgattc
     2101 gattgccaca acattttatt gggcaagaag aatatgagca gctaatcatg gtatttgaaa
     2161 atgtctcatc agaaactgag ctttcttata tgaaaaaaac agtaaatgaa tccaatgaag
     2221 tagatacatc ttggaacttc aagatacagc caaagcttga aatgacattg tattatcaag
     2281 gggatgtcaa atcttctcac gagaaggtac cctattctaa tacctccttt cgcgtaacgt
     2341 ccattgaaga atcgatcaaa ataaaagatt ttgaaggtga atttttcttg atgacgagag
     2401 aaaaagaagc ctatgaacgg ttcatttcac tattcactga tcgattcaat caagcatttc
     2461 acactgatta tgccactaat atttttgaag actttgaaaa tagctattcg tattcggtta
     2521 tttcaaatac agacgtatat aattatcgag tgtatttaac tattggattg attcttttat
     2581 tccttttgtt atcatccttt ttcttattga tgaataaaga aatcagttta ttgttgaata
     2641 atgacttttc attacatcaa gtattgtggt gtttattggg gaaaaagaaa atcattatta
     2701 cattcataat tgcaggaatc attggtggtt ttatttttat gtattataag gagcaatttc
     2761 tttatttgtt ccagatgtac gttttcttgt tcagcggaat atatggtatc tctttgattt
     2821 ctcttttatt agttgaaaaa atcgcttcgt taagagaagc gttcaactat atccaataca
     2881 ttctttttac atttggattg ttgttttctc ttcttttgac cgttgccaat gtcgaattag
     2941 caactatcgt tttttcttct tcacagttag ctgtttccca tgaaccacca aaagaaatcg
     3001 ctggtgagga ctatcatgta ttttatccag taaccatcgg gaaaaaccac gtggatttta
     3061 tttattccaa tcgttttgca tcagcagcag atcaggaatt atatgaaact ttgaataaaa
     3121 acggaagtat attagtcaat gtatcagatt acttgaatga agaaaatgaa cagtttggca
     3181 gagggataaa aataaatctg aattatctta agaaatttcc tttgattgat gtctctggaa
     3241 ggttgatcca aataaccgaa aaagaaacgc accctgttat attgatccct gaacgatata
     3301 gacatacaga tttaaaaaat gacctggcgc aaataacaga atattatcaa gaaatcagtg
     3361 aaaaagaacc gaaatttcta tttataaaaa acaatcaacc catttataca tttattcctt
     3421 ctatcccctg gatcgaacat tatccggttg ttgaaatact gacattgaaa aacagtatgt
     3481 atttggaaag aaatattctc agcggagaaa tctatccacc gttaaagatc aagataggct
     3541 ctttgtcgaa agaaaggtta tttgagctga ttgaagaaag tcagttacgt gataacttgc
     3601 agttatcatt tccgtatact cagactgaag agatagctgt caaagtgtta agcagatcat
     3661 ttcattactt gatacagata tttttactca ctttcttttc tttcttcttg ttgagctatg
     3721 tcatgttatg catatatttt gtctacaatt gtcggaaaat cgctatcttt cgttcaagtg
     3781 gttattcgct ctttgaaacg tataaagatt tcttcatgat gaacctcatc aaatggggaa
     3841 cgacaagtgt aatcttcctt tttcttatag aaagagagcc taaatatctt tttaatattt
     3901 ttttctttag cttcattgat tttattcttt cagttgtttt tattttgtct acagaaaaga
     3961 aaagtcagtt attgctgatg aatggaggtt aacaattgga gccgttgata gtgataaaag
     4021 gtgcgaaaaa aatcattcga aacaaaacga ttcttcattt ggatcgtttc actgcggatg
     4081 ctggggatct tgtgactgtt gttggagaaa gtggagcagg taaaagtacg ttattgaata
     4141 tattaggatt gaatgatcag ttcagttcag gacaattcta tttgttcgat caagagacga
     4201 aacaattaac tgggagagag aagcgcttga taaaaagaaa taccatatct tttctttttc
     4261 aagattttgg gttgattgaa tatgaaacaa tcgattttaa tttagatatt ggattgaagt
     4321 tttcgaattt aaatgtttca gataagcgaa cagcaaagta tgaggcattg aaaaaagtaa
     4381 atataaacaa gaagctaagt acgcctatct acacattatc tggtggtgag aagcaaagaa
     4441 tcgcctgtgc gagagtctta ttgaagaata gcaaaatcat tttagccgat gaaccgactg
     4501 ggtcattgga taaaagaaat aaggataaag tagttgaaat cttatcgcaa caagcttccc
     4561 aaggtacgtt agttatcgta gtcacgcatg atccatatct gaccaaaata ggaaacaaga
     4621 agatcatgct ttaaaaatca aaaaggaaag gatgtcgttt tatgaaaaaa gcagttatta
     4681 tttgtgcact agggataggt tttgttggag gaaacgctat gtcatctgaa gtggaagcaa
     4741 attcagttca cggtgaaatc tattacaaat ctttaaagcc aggagagatt cattttgatg
     4801 ctaaaggtaa tattattaag tcaaatttac cagtagaccg ggaaatttct agaacagcaa
     4861 ttaatcatag ctctggaaga tgggtttatt atagtgatac aacttgtacg agaaaaacag
     4921 gtcattctaa ccactatagt cgtgtctata atcgtcacgg tgcaaaagcg aaagttggaa
     4981 actctattcg atcagcaaat gcaacaaaag gagtatgggc ctatgcaagt gcgacaggaa
     5041 atagaactga cacattctca tgttggtaca atccaaccgg ttggtattaa gtatccaaaa
     5101 gtttatataa aatagaaaaa acatataatg aaacagcgtt aaaagttatt tttggtagtt
     5161 tt
//
LOCUS       DACDSR010000132         5066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDSR010000132 DACDSR010000000
VERSION     DACDSR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.135"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IWK15_002611"
     CDS             complement(406..780)
                     /locus_tag="IWK15_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9548143.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IWK15_002612"
     CDS             complement(803..2203)
                     /locus_tag="IWK15_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9548144.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IWK15_002613"
     CDS             complement(2207..2617)
                     /locus_tag="IWK15_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9548145.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IWK15_002614"
     CDS             complement(2635..3126)
                     /locus_tag="IWK15_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9548146.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IWK15_002615"
     CDS             complement(3136..3927)
                     /locus_tag="IWK15_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9548147.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IWK15_002616"
     CDS             complement(3920..4693)
                     /locus_tag="IWK15_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9548148.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5066)
                     /locus_tag="IWK15_002617"
     CDS             complement(4849..>5066)
                     /locus_tag="IWK15_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9548149.1"
                     /translation="INLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSKDFFS
                     EIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1616 a         1028 c          666 g         1756 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattc
//
LOCUS       DACDSR010000133         5043 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDSR010000133 DACDSR010000000
VERSION     DACDSR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.136"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..727
                     /gene="treR"
                     /locus_tag="IWK15_002618"
     CDS             14..727
                     /gene="treR"
                     /locus_tag="IWK15_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9548150.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            804..1250
                     /gene="msrB"
                     /locus_tag="IWK15_002619"
     CDS             804..1250
                     /gene="msrB"
                     /locus_tag="IWK15_002619"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9548151.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1420..2022
                     /gene="ruvA"
                     /locus_tag="IWK15_002620"
     CDS             1420..2022
                     /gene="ruvA"
                     /locus_tag="IWK15_002620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9548152.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2035..3036
                     /gene="ruvB"
                     /locus_tag="IWK15_002621"
     CDS             2035..3036
                     /gene="ruvB"
                     /locus_tag="IWK15_002621"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9548153.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3065..3643
                     /locus_tag="IWK15_002622"
     CDS             3065..3643
                     /locus_tag="IWK15_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9548154.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3695..4177
                     /locus_tag="IWK15_002623"
     CDS             3695..4177
                     /locus_tag="IWK15_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9548155.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4294..4668
                     /locus_tag="IWK15_002624"
     CDS             4294..4668
                     /locus_tag="IWK15_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548156.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1726 a          850 c         1097 g         1370 t
ORIGIN      
        1 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
       61 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
      121 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
      181 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
      241 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
      301 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
      361 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
      421 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
      481 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
      541 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
      601 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
      661 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
      721 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
      781 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
      841 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
      901 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
      961 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
     1021 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
     1081 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
     1141 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
     1201 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
     1261 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
     1321 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
     1381 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
     1441 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
     1501 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
     1561 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
     1621 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
     1681 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
     1741 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
     1801 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
     1861 acaaaagacg ctacagctct atctgaagca atagaagctc tctcggctct tggttacagt
     1921 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
     1981 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
     2041 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
     2101 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
     2161 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
     2221 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
     2281 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
     2341 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
     2401 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
     2461 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
     2521 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
     2581 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
     2641 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
     2701 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
     2761 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
     2821 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
     2881 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
     2941 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
     3001 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
     3061 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
     3121 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
     3181 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
     3241 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
     3301 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
     3361 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
     3421 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
     3481 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
     3541 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
     3601 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
     3661 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
     3721 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
     3781 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
     3841 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
     3901 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
     3961 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
     4021 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
     4081 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
     4141 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
     4201 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
     4261 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
     4321 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
     4381 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
     4441 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
     4501 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
     4561 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
     4621 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
     4681 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
     4741 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
     4801 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
     4861 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
     4921 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
     4981 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
     5041 gtc
//
LOCUS       DACDSR010000134         4846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDSR010000134 DACDSR010000000
VERSION     DACDSR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.137"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            54..380
                     /locus_tag="IWK15_002625"
     CDS             54..380
                     /locus_tag="IWK15_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014024983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548157.1"
                     /translation="MDFSTKKIIDDLYDTSDTMDRIILSYSQYLDKLENMINNMISYQ
                     LNMIMKTLTEISIVLTIPAIIFGFWGINVHVPFEKSSYGVLAVLIISAILSLICWLLM
                     RRKTYL"
     gene            898..1353
                     /locus_tag="IWK15_002626"
     CDS             898..1353
                     /locus_tag="IWK15_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9548158.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            1664..2020
                     /locus_tag="IWK15_002627"
                     /pseudo
     CDS             1664..2020
                     /locus_tag="IWK15_002627"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2033..2443
                     /locus_tag="IWK15_002628"
                     /pseudo
     CDS             2033..2443
                     /locus_tag="IWK15_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            2558..2722
                     /locus_tag="IWK15_002629"
     CDS             2558..2722
                     /locus_tag="IWK15_002629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548159.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(2873..3852)
                     /locus_tag="IWK15_002630"
                     /pseudo
     CDS             complement(2873..3852)
                     /locus_tag="IWK15_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3857..4624)
                     /locus_tag="IWK15_002631"
     CDS             complement(3857..4624)
                     /locus_tag="IWK15_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9548160.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT         1610 a          800 c          862 g         1574 t
ORIGIN      
        1 ctaaccataa agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt
       61 ttagtactaa aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca
      121 ttttaagtta cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt
      181 atcagctaaa catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag
      241 caattatttt tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg
      301 gtgttttagc cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc
      361 gacgcaaaac atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc
      421 tttgttgact ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc
      481 agaagtcaat aaattggact atttttattc agttacttag gcaacatttg agatacgatt
      541 gcgattataa aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc
      601 gatatcatcg aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat
      661 actagaaaac atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg
      721 aaaactgatt aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt
      781 taagtgatat aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata
      841 aacaatttaa ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg
      901 gtgcaacaaa ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca
      961 cttctcgata gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc
     1021 acgctactac gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac
     1081 ggctctgtca aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta
     1141 tatttaaagc ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat
     1201 atcaattctg agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag
     1261 cagtttcata aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa
     1321 gagaaaaaat tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc
     1381 ttagcgattt taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc
     1441 aaaattgacg tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc
     1501 catgtcagag gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat
     1561 ctaaccaaat tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc
     1621 aaaaataaaa aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt
     1681 tagagctagt tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt
     1741 tgggcattta gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa
     1801 acttgaatct ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg
     1861 aaagcagttt aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac
     1921 agatagcgac agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt
     1981 ttcaaaagat aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg
     2041 aagcggtttt caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca
     2101 aacacgccaa attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa
     2161 tttacaacag aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt
     2221 ataccttatt tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga
     2281 ggtggaagct aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt
     2341 aaaacccaag aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca
     2401 agaggggata attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt
     2461 ttctttaagt agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac
     2521 tttaaaagct atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac
     2581 accatttatt acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg
     2641 ttttttagga cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag
     2701 cacccaaatt ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca
     2761 tcctattcta atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt
     2821 tgtccaatat ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg
     2881 taagagtatc aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa
     2941 tttctgttac acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta
     3001 ttccttactc tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt
     3061 tattaaaact aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat
     3121 ttaattttcc attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg
     3181 ttccattata gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat
     3241 aatcaggatt gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta
     3301 tagccctttt agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat
     3361 agctattttc tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa
     3421 aatattgata ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt
     3481 tagaatacct attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg
     3541 aatcatcgta atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt
     3601 cctagaaata ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag
     3661 ctgctcaata ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct
     3721 gaaacgtttg taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac
     3781 gctacgttta aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt
     3841 tttataagtc ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta
     3901 agtgttgagg gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga
     3961 agttaaagaa atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag
     4021 cattgtttag ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat
     4081 tatgggtaac agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg
     4141 tcgaagccat tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca
     4201 ataaatcaat ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga
     4261 cacgaatttt ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc
     4321 caggtccaat aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt
     4381 caattttttc ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt
     4441 tagaggaaag caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag
     4501 catcaaggtc tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta
     4561 tggttatggt cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt
     4621 tcatatgaag acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt
     4681 gtatcataaa agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt
     4741 agatgatgaa ataagaacag aggattgacg tatattagct taggtcagat tttgtataag
     4801 acgaaaataa agtaggacct cttaatcagt aagttataga aagtaa
//
LOCUS       DACDSR010000135         4833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDSR010000135 DACDSR010000000
VERSION     DACDSR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.138"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(182..1144)
                     /locus_tag="IWK15_002632"
     CDS             complement(182..1144)
                     /locus_tag="IWK15_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9548161.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="IWK15_002633"
     CDS             complement(1146..2585)
                     /locus_tag="IWK15_002633"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9548162.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="IWK15_002634"
     CDS             2770..4716
                     /locus_tag="IWK15_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9548163.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1547 a          819 c          790 g         1677 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aat
//
LOCUS       DACDSR010000136         4800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDSR010000136 DACDSR010000000
VERSION     DACDSR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.139"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IWK15_002635"
     CDS             complement(<1..257)
                     /locus_tag="IWK15_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548164.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IWK15_002636"
     CDS             complement(273..923)
                     /locus_tag="IWK15_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548165.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IWK15_002637"
     CDS             complement(934..2106)
                     /locus_tag="IWK15_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9548166.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IWK15_002638"
     CDS             complement(2125..2997)
                     /locus_tag="IWK15_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9548167.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IWK15_002639"
     CDS             complement(3019..3939)
                     /locus_tag="IWK15_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548168.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IWK15_002640"
     CDS             complement(3939..4208)
                     /locus_tag="IWK15_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548169.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IWK15_002641"
     CDS             complement(4205..4585)
                     /locus_tag="IWK15_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9548170.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1257 a         1052 c          791 g         1700 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
//
LOCUS       DACDSR010000137         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDSR010000137 DACDSR010000000
VERSION     DACDSR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.140"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWK15_002642"
     CDS             564..989
                     /locus_tag="IWK15_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548171.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWK15_002643"
     CDS             2141..3118
                     /locus_tag="IWK15_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9548172.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWK15_002644"
     CDS             3128..3625
                     /locus_tag="IWK15_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548173.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWK15_002645"
     CDS             4001..4282
                     /locus_tag="IWK15_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548174.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWK15_002646"
     CDS             4284..4700
                     /locus_tag="IWK15_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548175.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDSR010000138         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDSR010000138 DACDSR010000000
VERSION     DACDSR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.141"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWK15_002647"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWK15_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWK15_002648"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWK15_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9548176.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWK15_002649"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWK15_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWK15_002650"
     CDS             1786..1869
                     /locus_tag="IWK15_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9548177.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWK15_002651"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWK15_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9548178.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWK15_002652"
     CDS             complement(2994..3857)
                     /locus_tag="IWK15_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9548179.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWK15_002653"
     CDS             complement(3859..4131)
                     /locus_tag="IWK15_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9548180.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWK15_002654"
     CDS             complement(4148..4363)
                     /locus_tag="IWK15_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9548181.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IWK15_002655"
     CDS             complement(4455..>4710)
                     /locus_tag="IWK15_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9548182.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACDSR010000139         4610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDSR010000139 DACDSR010000000
VERSION     DACDSR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.142"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IWK15_002656"
     CDS             complement(85..339)
                     /locus_tag="IWK15_002656"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9548183.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IWK15_002657"
     CDS             complement(450..704)
                     /locus_tag="IWK15_002657"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9548184.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IWK15_002658"
     CDS             complement(897..1172)
                     /locus_tag="IWK15_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9548185.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IWK15_002659"
     CDS             complement(1144..2097)
                     /locus_tag="IWK15_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9548186.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="IWK15_002660"
     CDS             2709..4202
                     /locus_tag="IWK15_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9548187.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="IWK15_002661"
     CDS             4337..>4610
                     /locus_tag="IWK15_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9548188.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DACDSR010000140         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDSR010000140 DACDSR010000000
VERSION     DACDSR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.143"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            323..1054
                     /locus_tag="IWK15_002662"
     CDS             323..1054
                     /locus_tag="IWK15_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9548189.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="IWK15_002663"
     CDS             1199..3226
                     /locus_tag="IWK15_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9548190.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="IWK15_002664"
     CDS             3334..4431
                     /locus_tag="IWK15_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9548191.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DACDSR010000141         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDSR010000141 DACDSR010000000
VERSION     DACDSR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.144"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWK15_002665"
     CDS             complement(543..2048)
                     /locus_tag="IWK15_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9548192.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWK15_002666"
     CDS             complement(2045..2731)
                     /locus_tag="IWK15_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9548193.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWK15_002667"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWK15_002667"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9548194.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDSR010000142         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDSR010000142 DACDSR010000000
VERSION     DACDSR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.145"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWK15_002668"
     CDS             690..1658
                     /locus_tag="IWK15_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9548195.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWK15_002669"
     CDS             1669..2484
                     /locus_tag="IWK15_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9548196.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWK15_002670"
     CDS             2552..3262
                     /locus_tag="IWK15_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9548197.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWK15_002671"
     CDS             3298..>4425
                     /locus_tag="IWK15_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9548198.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDSR010000143         4422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDSR010000143 DACDSR010000000
VERSION     DACDSR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.146"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(202..432)
                     /locus_tag="IWK15_002672"
     CDS             complement(202..432)
                     /locus_tag="IWK15_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548199.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            508..580
                     /locus_tag="IWK15_002673"
     tRNA            508..580
                     /locus_tag="IWK15_002673"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:541..543,aa:Lys,seq:ctt)
     gene            742..963
                     /locus_tag="IWK15_002674"
     CDS             742..963
                     /locus_tag="IWK15_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548200.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1287..1532)
                     /locus_tag="IWK15_002675"
     CDS             complement(1287..1532)
                     /locus_tag="IWK15_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548201.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1526..1927)
                     /locus_tag="IWK15_002676"
     CDS             complement(1526..1927)
                     /locus_tag="IWK15_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9548202.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2099..3907)
                     /gene="uidA"
                     /locus_tag="IWK15_002677"
     CDS             complement(2099..3907)
                     /gene="uidA"
                     /locus_tag="IWK15_002677"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9548203.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(3935..>4422)
                     /locus_tag="IWK15_002678"
     CDS             complement(3935..>4422)
                     /locus_tag="IWK15_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9548204.1"
                     /translation="VVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIK
                     NIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIF
                     VSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGISPIDAYCGLKDTF
                     I"
BASE COUNT         1364 a          808 c          576 g         1674 t
ORIGIN      
        1 aaaaattcat ctctaatttt ttctgctcgt tttttatttc ctttcttttt taattttgta
       61 tctttccact tttgtctaat ttttccgtta gagtctttat aagatacaac tacttgtaat
      121 gttcctcttt tttcaattat gtttgctctt aatttcaatt caatctctcc tcttaaactg
      181 aaatatcggc aaaaattttg actattttat tatagcatga tttttacctt catcttgtat
      241 tttcaatata tactcaatga cagctttttt aggtattttg taccttgctc caatttttat
      301 attttctatt gatctatctt tcagcaaatt ataagcttta ttttttccaa tatttaacat
      361 tttagttaaa tcagtaattt tcaatatcga tggataactt tcaaatattt tttcataatc
      421 ttgaaaatcc ataaaattca ctctcaattc tgataataag tattgcactt ttttatttta
      481 ggtgttagaa taacaaagta ttaatttggg ctgttagctc agttggtaga gcagatgact
      541 cttaatcatc gggtcgcggg ttcgagcccc tcacagccca ttgggtgcca aacccttaaa
      601 tacagtgtaa acgttggtgc ttagcattta gaaaacgtat atactgtatt tttttgtttt
      661 caaaagagac tgtagtaccc ctttttttca ctaaaagccc actgtagtca ttgatatttt
      721 tgataagaaa ggaacataat tatgcgtaat gaaattgcta atataacaat aatccgagaa
      781 actttacaaa accatactgc aaatgagatc tctaaacata caggattaaa tttgagtact
      841 ataaaaaaac taaaaagtgg agaacgttca atagaaaaac taaatttaca tgatgctatt
      901 tgcctcactg aatttggtct taaaaataat agaaaaaatg ttgaaatcaa catatggaaa
      961 taattcttaa tcagctgctt ttgtcaaaag cacataggaa tttcacctct ctccaagttt
     1021 acggcgagcc ttacggaagt atcattatcc caacttcaaa tcatcgcttt ttattgacta
     1081 taaaaattat tttagaatgt ttatcgctcc ttctttacag taagaaatca tggcgcaact
     1141 aaaatatgct ccttgaagtg ttaacgtact gatagaatgt atttaaattt caatgatcaa
     1201 taggttttct gtttaaaaga atctactaat cctttaacta ttagaagatt cttttaaaca
     1261 gaataagtgg aaaggggact ttttagttag ttaattttaa attttaagat cgcttctatc
     1321 aatttaattt ctagctgtct tagcatatac tcatctacat aggtaataaa ttgccccgcc
     1381 tcattaaatc catttgtcaa acataactta gctatataac tatgataatg tttgagtatc
     1441 atcttgattg aaattggatc ttcttctaga gcagattgga taatggatag aggaactaat
     1501 ggatactgcc gttttttggg gttgtctacc ataagtcata ccccattttt aattgttcaa
     1561 ctgccttctt tcgttgatac cagataccac ttgtactcat attgaggatt ttaccgattt
     1621 cagtatcatt aaaacctgca aaataataaa gtaggataac ctttcttctt atttccgata
     1681 aatgatcaat ggctgccgat aacttttgat ctgaaattaa tatttccatt ccaaataaga
     1741 aatagacatc ttgttcatca aaatgagttt gggagtactt accttcattt gaattcgtca
     1801 atggttcctc attatctgtt atgttaatgt ttcgttttcg aatagactta gtttcgtttc
     1861 gtaatacttt tttacagtag gcatcaaatt gatgttgtat agatttctca ttgtaatttt
     1921 gttgcatgat aaatatgctc cttttttccc ctttccacta atacaacaat cagaaagcgc
     1981 ctttttttcg aaaaaactag aaaaaaatat ccttctttaa acagaaaatc tttttataaa
     2041 aaaaaggagt tggcaattaa gcagccactc cttttttata ttccttataa aaggtaaatt
     2101 attttttata actgaaattt ggtattgatg accatcgttt ctttagatag tgagcaatca
     2161 ttttaggctt tctatctcta gtaaagattc ctttcttatt tccttgcact cttaaaatcc
     2221 cttgacttgt ttcaaaatct gcaaaattcc acacttgttc accaacaaaa ttgtcacatt
     2281 tatctattac ttcgtgattt gcttcgtaat aacccacttg atactcttct gtaaacattg
     2341 ttggtactgt atcatgaagt ccagctactg tatctgctcc atattctgta aacattattg
     2401 gcgtatcggg acatctttcc atccatccat ctagctcttt tttcaacaga tattttgcat
     2461 gttttaggtc tccacctgaa acataccaac cataatagcg atttaaacat aaaacatcga
     2521 caatatctcc tactttacaa atatctggtg ttgactttaa gtaagtaacg attgttacag
     2581 gtctcttctg agggtcaagt tctttagtca attcaactaa aggttcaaaa tattctttag
     2641 cgccttcaga atccgaatct ggttcatttg caatagacca cattacgaca ctcggatggt
     2701 tcttgtcgcg ttctatcaat tccttaatcg ccattttgtg agcattttct gtccgtattt
     2761 ctttccaagt atcatgtttt tctacgttgt catctaataa agtagccatg aaattcaaat
     2821 gtaatcctac tgcaggtact tcatcaataa ctacaatacc ttctcgatct gctaagcgca
     2881 taatttcttc tgaataagga taatgtgaag tcctaaatga attagcaccg atccatttca
     2941 ttaaattaaa atctttgata tttactgctt cattcaaacc acgaccatta atatacgtgt
     3001 cctcatgctt cccaaatccc ttaaagtaaa aaggtttatt attaatcaaa aacttcccat
     3061 tcgaaacctg aatactacga atgccaaatt cttctgtgta cgtatcaatc actttatttt
     3121 cgttaattaa actaactttc atactatata agtaagcatt tagaggctcc caaagtgttg
     3181 ggttcttaag ttgaatcact ccttctttcc ccttagcttc tgctataagt tgagcttctt
     3241 cgtcatagat tgctattaga atttctccgg tccattctgt atcgatatta aattctacat
     3301 ttgcatctga atcaattatt tttgtattaa tagtgacatc ctttatatag tctttcggag
     3361 ttgtatacaa tttaacgggt ctattaatgc ctgcataatt gaaaaagtca aaattaacca
     3421 aattttcttt ttttatgtct ccatcttcct gtatcgtttc tttcaaaatc cctacaggta
     3481 aagtgctatc gtcaataaca ttgtttacag ccactgtaat tctgttactt ccatctttta
     3541 taaactgatt aatttctgat tcaaatggcg taaaccctcc tttgtgttga ttaatcaatt
     3601 ttccattcaa aaatactttt gcttcatgtg ttgcagatcc aaaacgaaga actaatcttt
     3661 cttctaataa ttttttagga agaatcactt ctttttcata ccacacccat ccaacatgat
     3721 ttctaatgtt ctctgtttca aataaatcat tataagaaga aggaactgaa attggataac
     3781 tctctttttc aaccattggt tctaaagcaa gttcttcttg taagtcatca tttgtatcta
     3841 acttaaatct ccaaagaccg cttaaatcaa ttacttgcct cgtctctgtc ataatgggat
     3901 ataacatgta aatcactcct atataattta aatattaaat aaatgtatcc tttaacccac
     3961 aataagcatc tattggactt attcccttaa attcagaaag gcccattagt ttgtccacta
     4021 atatttctaa agtttcttgt tttccatcat aagtattgat ataagttttt acttgaggca
     4081 catcagctaa atgaaacgga cactgtacag atacaaaaat tgttggaatt tcatgaatat
     4141 aaaatggaat atccggagta cctttacttg ctggccaaac aattctttga actgtacctg
     4201 ggttaacatt agctatatta ataatcaagt catatttttc agttaaatct ttgattggtc
     4261 tcttagcagc atatattcca gctatatttt taattttttc ttcttgcgat aatcctttca
     4321 attcttcttc aggtgaaatg tatttttcaa cttcaaatcc ttttgctgac aaaatctgtt
     4381 tcaataaatc aatggcctga ggtctatttc cagcaactac tt
//
LOCUS       DACDSR010000144         4354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDSR010000144 DACDSR010000000
VERSION     DACDSR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.147"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1770)
                     /locus_tag="IWK15_002679"
     CDS             complement(<1..1770)
                     /locus_tag="IWK15_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548205.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1724..3478)
                     /locus_tag="IWK15_002680"
     CDS             complement(1724..3478)
                     /locus_tag="IWK15_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548206.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3588..4157)
                     /locus_tag="IWK15_002681"
     CDS             complement(3588..4157)
                     /locus_tag="IWK15_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9548207.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4175..>4354)
                     /locus_tag="IWK15_002682"
     CDS             complement(4175..>4354)
                     /locus_tag="IWK15_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548208.1"
                     /translation="LIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRV
                     EDSTLIEQIFNDITK"
BASE COUNT         1461 a          825 c          604 g         1464 t
ORIGIN      
        1 tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag
       61 atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt
      121 tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat
      181 aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt
      241 ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa
      301 agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc
      361 gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg
      421 agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa
      481 aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga
      541 agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc
      601 atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa
      661 cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt
      721 atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga
      781 tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata
      841 aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc
      901 tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc
      961 tactagaatg actactgcta aactaacata tgagaaaata gaggataagg ccatgactga
     1021 aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag
     1081 tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca attctatagt
     1141 ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt
     1201 ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc
     1261 taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc
     1321 cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga
     1381 catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt
     1441 tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata
     1501 taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac
     1561 ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat
     1621 tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt
     1681 agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat
     1741 tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat
     1801 gtttagccat taattcatta tgatttccac gttctactat tttcccttct gacaagacaa
     1861 gaatttcatc tacagattta atgctggata atcgatgagc tatcataata actgtttttt
     1921 ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg
     1981 cactggcttc atccataata acaatctttg catcttttaa aattgctctg gcaatagcta
     2041 tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc
     2101 tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt
     2161 tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata
     2221 gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat
     2281 ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta
     2341 gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt
     2401 gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat
     2461 tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt
     2521 gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt
     2581 gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca
     2641 acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta
     2701 taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg
     2761 ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca
     2821 aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat
     2881 attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg
     2941 tattctctgt cattttcttt aacaagaata caccaattac taacatggcg attagaacca
     3001 atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg
     3061 cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat
     3121 caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg
     3181 ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag
     3241 aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa
     3301 ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt
     3361 aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta
     3421 accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt
     3481 tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta
     3541 atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat
     3601 ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac
     3661 actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca
     3721 gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc
     3781 cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct
     3841 cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg
     3901 ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg
     3961 taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca
     4021 gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta
     4081 accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata
     4141 taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct
     4201 gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt
     4261 ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga gtagctataa
     4321 ctactgcacc cttttctttt aagtctttaa taag
//
LOCUS       DACDSR010000145         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDSR010000145 DACDSR010000000
VERSION     DACDSR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.148"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IWK15_002683"
     CDS             complement(65..1285)
                     /locus_tag="IWK15_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9548209.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IWK15_002684"
     CDS             complement(1305..1910)
                     /locus_tag="IWK15_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9548210.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IWK15_002685"
     CDS             complement(1959..2891)
                     /locus_tag="IWK15_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9548211.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IWK15_002686"
     CDS             complement(3312..4172)
                     /locus_tag="IWK15_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548212.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDSR010000146         4244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDSR010000146 DACDSR010000000
VERSION     DACDSR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.149"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..593
                     /locus_tag="IWK15_002687"
     CDS             <1..593
                     /locus_tag="IWK15_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9548213.1"
                     /translation="QKQIYEISKNQTSILELLDGYNSDLISEYKNEIETCVNEIRKLN
                     WDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEA
                     LKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDS
                     YRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            596..1492
                     /locus_tag="IWK15_002688"
     CDS             596..1492
                     /locus_tag="IWK15_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9548214.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1542..1931
                     /locus_tag="IWK15_002689"
     CDS             1542..1931
                     /locus_tag="IWK15_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9548215.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1918..>4244
                     /locus_tag="IWK15_002690"
     CDS             1918..>4244
                     /locus_tag="IWK15_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9548216.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         1463 a          655 c          884 g         1242 t
ORIGIN      
        1 ctcaaaaaca aatttatgaa atttcaaaaa atcaaactag tatactagaa ttactagatg
       61 gatataactc tgatttgatt tcagagtata aaaatgagat tgaaacatgt gtaaacgaaa
      121 taagaaaatt aaattgggat attgtttctg tgaaaaaaga aatacaagat aaggcgaagg
      181 tagagcttga aattgaagat ttaaaaaaac aagtagaaaa gctatctcat aagagttata
      241 aagatgttta tgatgagtat tcaaaagaga cagatattta tagggaactg aaacgtgatg
      301 tggaggcact taaggaaatt cccaaaaatc taactgatag tgttgacaaa attgactttg
      361 gcaatggtat aaaagttaca gacgaaattg atgaacaccg cggagaattg attaagaatt
      421 tagtgaccaa tcgggctaag attcaaacaa taattaatga aatgtcagat gaaattgata
      481 gttatagagc agagctgaat aaatcagatt ggaaagttaa ctataatgag gtttcaaaga
      541 ggtatcaaag aggtatcaaa aacttagtga agagttatct caagatgaat taaaaatgtt
      601 acaaaacctt gattctttat atcaatcttt atcaaaaact gaagagcgat tggaagaaat
      661 tattcaacaa gaaataaagc ttaaagatta ccaagttgag gttgactcag caatgtcaag
      721 aatgaataaa tcatatcaaa atctctattc tgagagaagt agttcctcgc gcgaaatttt
      781 ttctgatatt acaggaatag aggctactat tcaaccaaat agggatttca ataattatgt
      841 taaagagctc agagcttgta ttaataagga ggacactttt gacagtgagt ttggatctct
      901 tattgataag ctgacaaaaa attccattac taatcaagat atatataatg acatacttga
      961 tataagagtt agtggggaaa gtagtatttt ttcagataaa aaattaatca attctataag
     1021 aaaattgaat gatgcacaag ttcttgaatt acaaaccctt aagccaaatg atttagtagt
     1081 tatccgactt gaactaaatg gaaaaaaagt tagtttatcg aatgctagtg ctggtcaaaa
     1141 gacatctgca attctttcta tgattttggc gtatggaaat tcaccactca tattggatca
     1201 gcctgaagat gatttggata gtcaattaat taataactta atcgtaagaa gtatcattac
     1261 aaaaaaggaa aacagacaaa ttttaattgt aactcataat gcaaatatcc cagttaatgg
     1321 tgattcagaa tggttgatta gcatgggaga ttcaaaagaa atatcggctg atcagtgcgg
     1381 gtcggtagat gaacccaata tcaagccaag aatatgtact gtgatggaag gtggcgagga
     1441 tgcttttaca aatagagcga aacgctatgg ttttaaacaa attgtagaat aacactagag
     1501 ggtgagacat attattatgt ccgccctttt taggaggaaa aatgaaaaaa ataaaaagct
     1561 ataccagtat ctgggcagtt gaaaaagtca tttacgcaat caatgatttt cagttgcctt
     1621 ttcctgtgac ttttaaccaa atggcttggt tcgttttatc tttactgttt gtcatcgtat
     1681 ttgcccatgt gcctccacta tctatgatag aggaggtctt tttaaaatac ttaggcatac
     1741 cggtggctgt cacttggttt atgtcgcaaa agacgtttga tggcaagaaa ccactgggat
     1801 ttttacgatc ctttatcagc tatcacttgc gatttaaggt gacttttgca ggaaagaaag
     1861 tcaaagaaca gaagaaacga tgggatgaac ccagtactct tgtaaggagt gtgaactatg
     1921 tatccgatta agtatattga aaataatctc gtctttaacc aagaaggcga gtgttttgcc
     1981 tactatgaat tagtgcctta caattactcc tttttgtcac ccgaacagaa atttcaagtg
     2041 catgataact ttcgccaact aattgcgcaa aatcgtgaag gcaagatcca tgcgttacaa
     2101 attgccacag aaagtagtat tcgcgccaca caagaacgat caaaaaaaga aattaccggt
     2161 agactcaaag aagtagcgaa acaacgaatt gatatacaga cagatgcatt agtatccatg
     2221 attggcgata gtcagattga ttatcgcttt tttattggct ttaaactaat tgcgacagat
     2281 gaagaagtca atctaaagag tctcaagaaa tctttctttt ctgggcttca agaatttgtc
     2341 tatggagtga atcatcattt gatgggcgat tttgtttcct tatccaatga agaaattcgc
     2401 tgctattcta agcttgaaaa attaatggaa agcaaattgg ctcgaagatt taaagtcagg
     2461 cgagtcacac cgagtgacct gacgtatttg attgaacata tttatggcga aaaagggata
     2521 ccgtttgaag aatacgaatt tcagctacca aagaaaaagc taaaatcaga aacactggtg
     2581 aaacgttatg acttattacg tccaaatcgt tgtttaattg aagaaaaacc aaggtgctta
     2641 cggatggaac atgagaatca tgagtcgtac gtagcgtatt taacaattaa tacaattgtg
     2701 ggggagatgg aatttccttc ctcagaactt ttctattacc agcaacaaca atttacattc
     2761 ccaatcgaca caagtatgaa tgtagagatt gtgacgaata aaaaggcttt agctaccgtt
     2821 cgtaataaga aaaaagaact gaaagattta gacaatcatg cttaccaatc tgacaatgaa
     2881 acgaattcca atgtcttaga tgctttggat tccgtggatg aattggaaac caccttggat
     2941 cagtcaaaag aatctatgta caaactgagt tatgtggttc gagtgagtgc cgaatcagtg
     3001 gatgaactaa agcgacgctg tgatgaagtg ctcgattttt atgatgatac caatgtaaaa
     3061 ctagtccggc cctttggcga tatgatgggg ctacatgaag aattcttacc ctcaagtaaa
     3121 cgatatatga acgactatat tcagtatgtt acgtctgatt ttttggcggg gcttggtttt
     3181 ggggctactc agatgctagg agaattagaa gggatttact ttggctacaa tgtggataca
     3241 ggtcgtaatg tttacctgaa accggcattg gcttcacaag gagtcaaggg ttcagtaacc
     3301 aatgcgctgg ccgccgcttt cctgggttct cttggtggag gtaaatcctt tagtaacaat
     3361 ctcttagtct actatgctgt tttgttcggt ggtcaagctg tgattgtcga tccaaaaggg
     3421 gagcgaggag ggtggaaaga aaccttgcca gaaattgcgg atgaaatcag tatcctcaac
     3481 ttgacgagta agtcagaaaa tcaaggtctg cttgatcctt atgtgattat gaagaaaaag
     3541 aaagactcgg aaagtttagc aattgatatt ctgacttttt taacaggaat ttcaagtcga
     3601 gacggggaga agtttcctgt gttacgtaaa gccattcgaa cggtgactca aagtgatcaa
     3661 cgggggttat tacaagttat taacgaattg cgaaaagacg ggtcaccaat cgcggaaaat
     3721 atcgccgatc atatcgagtc ctttaccgat tatgattttg ctcatttgct tttctcagac
     3781 ggctcgatta cccaatcgat tagtttagat aaacagctaa atatcattca agtggcggat
     3841 ttggttttac cggatgcaga aacgactttt gaagaatata ccaccatgga attgcttagt
     3901 gtttcgatgc taatcgtcat ttccactttt gccttggact ttatccatag taaccgagaa
     3961 atcttcaaaa ttgtcgattt ggatgaggct tggagttttc ttcaagtggc tcaaggaaaa
     4021 acgttatcga ataagcttgt tcgtgctggt cgggccatga atgctggggt ctatttcgtc
     4081 actcaaaata gcgacgattt attagatgaa cgtttaaaaa ataatatcgg gttgaagttt
     4141 gcttttcgca gtaccgatat tcatgaaatc aagaaaacct tggaattctt tggtttggat
     4201 caggaggatc aaagcaatca aaaacgcctg cgagatttgg aaaa
//
LOCUS       DACDSR010000147         4187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDSR010000147 DACDSR010000000
VERSION     DACDSR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.150"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            9..731
                     /locus_tag="IWK15_002691"
     CDS             9..731
                     /locus_tag="IWK15_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9548217.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="IWK15_002692"
     CDS             789..2828
                     /locus_tag="IWK15_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9548218.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="IWK15_002693"
     CDS             complement(2937..4088)
                     /locus_tag="IWK15_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9548219.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1529 a          781 c          810 g         1067 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaa
//
LOCUS       DACDSR010000148         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDSR010000148 DACDSR010000000
VERSION     DACDSR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.151"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWK15_002694"
     CDS             <1..106
                     /locus_tag="IWK15_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9548220.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWK15_002695"
     CDS             970..1182
                     /locus_tag="IWK15_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548221.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWK15_002696"
     CDS             complement(1460..3259)
                     /locus_tag="IWK15_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9548222.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWK15_002697"
     CDS             complement(3383..3979)
                     /locus_tag="IWK15_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9548223.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDSR010000149         3865 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDSR010000149 DACDSR010000000
VERSION     DACDSR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.152"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..630
                     /locus_tag="IWK15_002698"
     CDS             <1..630
                     /locus_tag="IWK15_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548224.1"
                     /translation="VPEYNLNHHLQINNYLIKGMALAKDHPHLKRIISERRTVYETKD
                     LNPKTKGRKFFVPDFIFRFRDKENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTR
                     PESHLIYVTPSGAIQEELHAFKRIFFKRETEKDASQQELFERLHIFSALEFEEGLKAL
                     LENDPAVNWRTVEREKIYPNGAVQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1117..1383
                     /locus_tag="IWK15_002699"
     CDS             1117..1383
                     /locus_tag="IWK15_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548225.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            1455..1796
                     /locus_tag="IWK15_002700"
     CDS             1455..1796
                     /locus_tag="IWK15_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548226.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            1851..2270
                     /gene="ssb"
                     /locus_tag="IWK15_002701"
     CDS             1851..2270
                     /gene="ssb"
                     /locus_tag="IWK15_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9548227.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            2347..2643
                     /locus_tag="IWK15_002702"
     CDS             2347..2643
                     /locus_tag="IWK15_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548228.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            2658..3089
                     /locus_tag="IWK15_002703"
     CDS             2658..3089
                     /locus_tag="IWK15_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548229.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3271..3576
                     /locus_tag="IWK15_002704"
     CDS             3271..3576
                     /locus_tag="IWK15_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548230.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1433 a          587 c          799 g         1046 t
ORIGIN      
        1 gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat taaaggaatg
       61 gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg taccgtctat
      121 gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc agattttatt
      181 tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat tgagctaacg
      241 ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg tgagttgcga
      301 acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca agaagaactt
      361 catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag tcaacaagaa
      421 ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt aaaagcatta
      481 ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat ttatccgaat
      541 ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga ggaaaaacca
      601 aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct ctttttttgt
      661 gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt taggaaggac
      721 taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc taatcgtata
      781 gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg gtaaaatgca
      841 cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc cgaacgaccg
      901 ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact gaaacccaaa
      961 atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg ggttgcaccc
     1021 ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc gaaaaaatca
     1081 aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa cgagcaactg
     1141 aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg gagctttgaa
     1201 ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc tacagcctta
     1261 gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa tggttttccg
     1321 caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga aaacttctta
     1381 taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa tataaagaaa
     1441 gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt ttttatagta
     1501 gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta acagaaaaaa
     1561 cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc atgagtggaa
     1621 aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact aaagacagta
     1681 ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt tatcgtagtt
     1741 gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat tattaagggg
     1801 atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc atgatgaatt
     1861 cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca ccaaaaggca
     1921 atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca aacggcgagc
     1981 gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact ttagcaaaat
     2041 atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg agctatcaga
     2101 atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc tatttgttag
     2161 agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa aataattctt
     2221 caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa gtgaaaaata
     2281 attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga gatgaggcga
     2341 ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt gggctcgttt
     2401 gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta tcaaagcgtt
     2461 cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa ctttttagat
     2521 tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc gaaacaaaca
     2581 ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga aaacaatgat
     2641 taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg ctagcattga
     2701 ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc ttatcggaaa
     2761 taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa gtagaaatag
     2821 tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat ggaaccaggc
     2881 aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca cttttgcata
     2941 aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact ggctggaaac
     3001 aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt ggctatcaac
     3061 gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc ttaagattaa
     3121 tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga atcaactggt
     3181 ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc taggtaaagg
     3241 cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg acgctataaa
     3301 ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta cgggtctata
     3361 accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc gaaaagtaag
     3421 aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag agttggtaaa
     3481 acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac agaagaaata
     3541 gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag aataaaaaaa
     3601 tcctctacag aaaactacgg agggcaacat gtgctattgt ttcaacaata catttttaat
     3661 ttttaccata acaaaaaagt acttgaaaaa aaacagaaat atgttaaatt aaaaatgaaa
     3721 aaatggaagc ggttacttat tcttttgaat ctaagataaa aattcaaaag aataacataa
     3781 cccaaatatg attatacttg agattctaaa tatttatcga aaaggaattt gttttaagga
     3841 aaaatgattc tatggacggt tattg
//
LOCUS       DACDSR010000150         3713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDSR010000150 DACDSR010000000
VERSION     DACDSR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.153"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWK15_002705"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWK15_002705"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9548231.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWK15_002706"
     CDS             complement(2261..2512)
                     /locus_tag="IWK15_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9548232.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IWK15_002707"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IWK15_002707"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9548233.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IWK15_002708"
     CDS             3462..3680
                     /locus_tag="IWK15_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548234.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DACDSR010000151         3473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDSR010000151 DACDSR010000000
VERSION     DACDSR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.155"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            53..1429
                     /locus_tag="IWK15_002709"
     CDS             53..1429
                     /locus_tag="IWK15_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9548235.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            1440..2234
                     /locus_tag="IWK15_002710"
     CDS             1440..2234
                     /locus_tag="IWK15_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9548236.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            2247..>3473
                     /locus_tag="IWK15_002711"
     CDS             2247..>3473
                     /locus_tag="IWK15_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9548237.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEK"
BASE COUNT         1210 a          422 c          691 g         1150 t
ORIGIN      
        1 tggcagttca acagaatgaa aacgcttaac aataggaagg agcgcaaatt atatggaaag
       61 aaaatttggt ctaagagaca aaataggata tatgtttgga gattttggga atgatttcac
      121 tttcattttt gctagttctt ttttaatgat tttttataca aaagtattgg ggataagcgg
      181 agctaccgta ggaacattat ttttagtagc aagatttatt gatgcagtta cagacataac
      241 aatggggcgt attgtggatg gatcaaaacc atctaaaact ggcaaattta aaccgtggat
      301 tttacggatg agtggtccag tcgctttagc aagtttttta atgtatcaaa catcaatggc
      361 atcagcttct atgagtatta aagttatata tatgtatgta acatatatac tatggggaag
      421 tattttttat acatctatta atattcctta cggttcaatg gcatcagcta tcacttctgt
      481 tgcagaagaa aggacagcgt tatcaacatt tcgtagtata ggagctacct tagcaggtat
      541 gttcattggc gtgttaacac caatttttat ttatacaaga gatagttctg gagctcaaat
      601 tgtacgtggt ggatcaactt ttacaattgt ggcaggagta ttttcagtgt gtgcagttat
      661 ttgttacgta atatgttata aattaactgt agaacgggtt aaaagtgatg aatcgcagtt
      721 aaaagtttca ttgttgaaat ctttaaaagg attagctaaa aatagagcgt tgctttctat
      781 aatagctgca gctatttttt tgttgcttgc tcagctttta acaatgagta tgaacaatta
      841 tgtatttcca gattattata ataatgcaca gggaatagtt ttaatgaact ttatcaatcc
      901 tattttgatg ttggtacttg tttcacctgt aagtttaatt ttcagtaaaa aatacggtaa
      961 aaaagaattg gcgtcagtag ggatgttctt ctctgctatt gtatatttac ttttattttt
     1021 gataaagcct agtaatatgt atgtattctt agttcttact tctgttggat atatggggtt
     1081 aggtattttt aatacagtca tttgggcgaa tattactgat gtaatcgatg atcaagaagt
     1141 gaaatctggc caaagagaag atggaactat ttatgcagta tattcttttg cccgaaaaat
     1201 tggacaagct ttagctggcg gtgtcggagg ttggactctt tctataatag ggtatgattc
     1261 gttagcaaaa gtacaaagtg gagaagtttt acaaaagctg tataactctt caacgttgat
     1321 tccagcagtt tgtttctttg ttgtaggatt gattttattt ttcgtatatc cattatctaa
     1381 agaaaaagta ttagaaaatt ataatttgct aaatcaaaaa cataactagg agaaaaaaca
     1441 tgaacagttg gatattttgt attgattcag atggttgtgt catggataca atgacttata
     1501 aacacaatct ctttttcggg ccaattgctg cagagatttt tgatgttcat catagggaaa
     1561 aattcctaca ggattggaat aatattaatt tatattcaga gactcgtgga ataaacagat
     1621 ttttaggttt agttaaaaca ttagaaaaaa atgatgtacc atcgattaat cgattaaagc
     1681 actgggtatc tgtaacaaaa gaattgtcta atccatcttt gaagagggaa atagaatact
     1741 ctcagtcaga ggatttatta aaagctttaa aatggtcgga agaagtcaat cgacagatta
     1801 gtcaagctaa aggattagat aaaccctttt taagtgctag agatactata aaagctctga
     1861 aaaaatatgg gaagataatt attgtaagct cagctaataa ggaagctatt caagaagaat
     1921 gggaaagaca tgaattatta tccttagttg atgaactgtg ttgtcaagat aaaggaaaaa
     1981 aagaagacat tattcgaagt gtaattgaaa atggttgtga tttggataag atattaatga
     2041 ttggtgattc tcctggtgat ctagaagctg caaataagaa taaagttttc ttttatccta
     2101 ttttggttaa taaagaaaaa gaatcatggg aaaacttaag aactgatatt ttatttaaat
     2161 gtttatcggg agagtatata gatattgaac aaaaatatat agatactttt tggaaaaatc
     2221 ttactaataa gtagaggaga aagaaaatga ctataaaagt agacttacga aaaaaaccat
     2281 atcatttaga tgaagatgct atttcttgga ttgaagagac gattgatgct atgactttag
     2341 aagaaaaaat aggtcaatta tttgttaata tgggatctag tcgtgataaa aattacttaa
     2401 gtagtatggt caatcagtac catattggag ctgtacgata taatccagga actgctgatg
     2461 aggtatatga tcaaaactat atcctacaaa cagaaagcaa aattccttta ttaattgctg
     2521 ctaatacaga agcaggtggt aacggagctt gtacagatgg taccgaagtt ggtgtagaag
     2581 taaaaattgg tgcaacaaat gatgcgaaat atgcttatga attagggaga gtttctggaa
     2641 ttgaagcatc ggctattgga tgtaactgga gttttgctcc tattgtagat attaatagaa
     2701 attggagaaa tcctatcatt tctagtcgat cttttggaca aagccctgat aaagttttag
     2761 aacttagttt agcttatatg aaaggtattc aagagagtgg aattttgcct gcagctaaac
     2821 atttccctgg tgacggtatt gacgaacgag atcaacatct atcttttagt attaatagtt
     2881 taagtacaga agaatgggat aattcttttg gaaaagtata ctcaggctta atagaagctg
     2941 gattaccttc aattatggca ggtcatatcc atatgccagc ttattcaaaa cattttaatc
     3001 ttgatctgaa agatgaagat ttacttcctg caactttatc aaaagaatta atcactgact
     3061 tacttagagg aaaactcggg tttaatggat tagtagttac tgatgctagt catatgttag
     3121 gaatgactgg ggcaatgaaa cggtcagaat tattgcccac ttctattgcg gctggatgtg
     3181 atctattcct attttttaat gatccagatg aagactttaa ttatatgatg gaaggttatt
     3241 acaaaggaat tatttcagaa gagcgtttac gtgatgcatt aagaagaatt ttaggcttaa
     3301 aggctaaact aggtttgcat aaaaaagaaa agaataaaat tttgttaccg aaacaagaag
     3361 ctatggaaaa aattggttta attgaaaata aatcaatttt taaagaggta gctgatcaag
     3421 caattacact agttaaagat aaacaagata tttggcctat tgtaccagaa aaa
//
LOCUS       DACDSR010000152         3449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDSR010000152 DACDSR010000000
VERSION     DACDSR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.156"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWK15_002712"
     CDS             complement(150..1337)
                     /locus_tag="IWK15_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9548238.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="IWK15_002713"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="IWK15_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="IWK15_002714"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="IWK15_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9548239.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3449
                     /locus_tag="IWK15_002715"
     CDS             3305..>3449
                     /locus_tag="IWK15_002715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9548240.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DACDSR010000153         3416 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDSR010000153 DACDSR010000000
VERSION     DACDSR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.157"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            166..1206
                     /locus_tag="IWK15_002716"
     CDS             166..1206
                     /locus_tag="IWK15_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9548241.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2030..2362
                     /locus_tag="IWK15_002717"
     CDS             2030..2362
                     /locus_tag="IWK15_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9548242.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2981..3322
                     /locus_tag="IWK15_002718"
     CDS             2981..3322
                     /locus_tag="IWK15_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9548243.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1271 a          461 c          647 g         1037 t
ORIGIN      
        1 taatgacttt aaaggtagta cataattcat tttatttctg attttaggta gtatttaatt
       61 aaaaaacagt tttaatgcgt ttgcattttt tatttatctt tgctattttc gaaaagagta
      121 ggtaatttta cataaataaa acaacgtgaa agtagaggat ctacaatgac tgattttaaa
      181 ttttttaagg cggatagagt ttacaacgaa ttattttatc aatttccaaa agtctttatt
      241 gtttctgacg aatacaaaaa aatgaaagat tcaactaaga ttgcctatat gcttttaaaa
      301 gcaagattag agatcgcaat cagcaaacgg caaatcgatg aagaaggtaa tgtttatttt
      361 acttatacga caaatgaact atgtagagta ttaaactgcc aaaaacaaaa agcgatagca
      421 atcaaaaaag agttggaatc ctttggttta ttattacaaa agcagatggg atttaacaaa
      481 cagttaggga aaaataatcc taatagacta tatctagcag aattaaaagt ctcagaaaat
      541 gatatctact tactcgaaaa atttgataga gagaataggg aaaacgttga taaatcagag
      601 ggtatgaaaa tcatacccac cctcgacgaa aaatcagacg ctgaatccct tggggctcaa
      661 gagggtatga aaatcatacc gtgccaaaac gttgataaat cagagggtat gaaaatcata
      721 ccagaactta ataataatat attagacact aatagacaca atatagacac tgaaaaagac
      781 cgcctacaag atcaattgtt gttagacaat tttgagacaa ttatgacaaa cgacagcatt
      841 gctacgtttg tccctgaacg agtattaaat ttgataaaaa cattttcttc aagttacagt
      901 gaagctcaaa aaaccgtcca gactattcat aatgcaaaga aaaaagctga aatagaaagt
      961 ggtatttcga tagtttttga agaactcgat agttattatg tcaatgcaga acaagaatta
     1021 tacacgacac tgttaaaagc ctatcaaaaa ttaaaaaccg aaaaagtcga aaatatccag
     1081 aacctgattt ttgtctatgt aaaaaattgg tttatcgaaa aaccaatagc tgctaaagta
     1141 tcaagtgaaa aacgtttgaa ttatgaaagc tccccaagca ctattacgaa agactggtta
     1201 gagtgaaact ttactgacaa agtgattgtt gcttcatgga gtaacattaa aattaaaaat
     1261 tttttattat tctaattata ttacgaatat tacaaaataa tagctatttt aatagaattt
     1321 ataagttaaa atattatgat aaaaatcaca aacgtagcct tttacgtcat aagtgttaaa
     1381 agaaataatt aatttcaaat atagaaaagg gaacgtatgt ttgcatactc cttttttttg
     1441 agttataata atacgcgaag agagataatt tcattattta aacaagctaa aaatcttgat
     1501 attacttctt ttcaggcaat acgaatatta ttcgcttaca ttttttgtat aaatttaatt
     1561 gtctgctatg cttgtgatga attgattttc gttctgcact tttggatgat aaatttggac
     1621 aattgaaaat acaaaaaaaa gaaaggcggt atgaactcgg aaggctggta accttcgtca
     1681 gcgttcatac acggcattaa gatttggccc ttaatgcgtt aacctttcag tgttacggta
     1741 ttacccgcaa tggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt
     1801 gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcacaa tgttataaaa
     1861 atgtccgtgt ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttgg
     1921 atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata
     1981 acaagaaaaa cttgttaaaa atatttaaag aaattgggtg gtttatttta tgtcatatac
     2041 aggaattttg agtctcaaag atatttgtca ctacggtaaa cggtgtaccg ctacagagaa
     2101 aatcacgaag aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta agaaatatat
     2161 aatccagaaa gacaaagtct cagaggaaat gatttattac attgggaaac aaaagcaaat
     2221 aattttaaaa gatcctatcc ccttaaaaga gctatatcca acaatcaaac atgtttatga
     2281 ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg
     2341 gatggggcgt ttgatcggct gaattgatca ttctcataac ttgtatttta tcgtttttcg
     2401 cttgcttgaa aatattgtcg agacttctaa aggaacttag agtcactaga aaaataagga
     2461 gtagacttga tctatcaaca attcccttcg gggagtgacg gggtaggttt taacgattgg
     2521 taacgtaaaa aattggtcaa tcgagtaaat gcgcttgttt ggatagtggt gaaatgaatt
     2581 tgtgtaaata ataagcgcag agtaaacaag agggaattta gaagttagac aagttagaat
     2641 agcgtctaga ttaatcatga gcagctacta agggatctag actttgacgc aaactcatgg
     2701 aataaggagt aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc
     2761 agtacatgta gatacttgcg aggacgttga ttattggatc aatcgagcat ttaccgctgt
     2821 ctactagggt ttctcgtttc ggacgccata aacgagtcga aattttttat gtatttttaa
     2881 gtaaacgaaa aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa ttgatataaa
     2941 acatagagaa aatgaaattt acgtaatgga ggaatgacaa atgaaaaatt atgatccgaa
     3001 tatacgattg ggcacacata ctattaaggt ttctttccaa cgttgggatt acaaaggatt
     3061 tgtgacgttt agaagaggcg gaaattgtaa aggattagat gttttagcat tagacgagga
     3121 tgatttatac gatcaaacat tgactgataa tccaattgga ttcggcttgc tacctgaaga
     3181 tgatgaaggt aatgaatggt ttaaaatgac gcttatgaat gataatggag acgaattatc
     3241 tgttgaagat acttggtcat atttgagtga ttatattgtc agctttgaaa tcattgaatt
     3301 tgtagcggat aaagaagaat aaaaactgga ggaataaatt ttacagataa aggcttagaa
     3361 ggagtcacga tgattaataa aaaggtagta ttaccttagg cattgagtaa ggaatt
//
LOCUS       DACDSR010000154         3378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDSR010000154 DACDSR010000000
VERSION     DACDSR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.158"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IWK15_002719"
     CDS             68..751
                     /locus_tag="IWK15_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9548244.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IWK15_002720"
     CDS             753..1589
                     /locus_tag="IWK15_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9548245.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IWK15_002721"
     CDS             1600..3354
                     /locus_tag="IWK15_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548246.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         1065 a          680 c          639 g          994 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaaga
//
LOCUS       DACDSR010000155         3350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDSR010000155 DACDSR010000000
VERSION     DACDSR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.159"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..748
                     /locus_tag="IWK15_002722"
     CDS             <1..748
                     /locus_tag="IWK15_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9548247.1"
                     /translation="ILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQKIGFSGFSDLKI
                     EIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYI
                     FGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDT
                     YDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDL
                     LVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            978..1208
                     /locus_tag="IWK15_002723"
     CDS             978..1208
                     /locus_tag="IWK15_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9548248.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1431..1529)
                     /locus_tag="IWK15_002724"
                     /pseudo
     CDS             complement(1431..1529)
                     /locus_tag="IWK15_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1794..2249)
                     /gene="tnpA"
                     /locus_tag="IWK15_002725"
     CDS             complement(1794..2249)
                     /gene="tnpA"
                     /locus_tag="IWK15_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9548249.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2492..3350
                     /locus_tag="IWK15_002726"
                     /pseudo
     CDS             2492..3350
                     /locus_tag="IWK15_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1106 a          579 c          584 g         1081 t
ORIGIN      
        1 tattttaaat tctgggaaaa ctattattta tagtacgatg agtgatatta agaaaaacac
       61 aaaagttggc gatgctacta ttattcgctt ttgtcaaaaa attggttttt ctggtttttc
      121 tgatttaaaa attgaaattg caaaggaaga ctttagtcaa aaaaaagaac atccgactag
      181 tgtaaagtac tatgatgaaa ttgctaatag tttaacagaa gctctctatt ccactattag
      241 gcttttagac gaagaaaaat tgaatgaagc cattaaaatg atcactcaat caaaaaacat
      301 atatatattt ggagtgggat ctagtggaaa tactagtctt gatttagaaa atatgtttct
      361 tcgcgtagga gttcaagcaa aagctgtact agaccctcat tttcaagccc aagtcgcttc
      421 tttattaaca gttaatgatt tagtaatcat tttttcttta tctgggaaaa cgaaagatac
      481 ttatgattca ttgaaaatag ctaaaaaaaa tggcgcaaaa attattgcta taactaacta
      541 tattcattca cctataggaa agtctgcaga cttagtttta caaacagcca ttgaagaatt
      601 tcttaatggt ggatctttag ctggaaaaat atcacaactt tatatttgtg atctattagt
      661 tcatggttat gaacaaaaca ataatattga ttcagttgaa ttaagagaaa aagttttgcg
      721 ctctattatt gataaaagta ttgagtgaac tacccccact tagctaaacc taacggtttt
      781 taacgattga agtaggcaat tcttgggatg agagcatact tgatagctta ttttttacta
      841 atagcggtgg ctcttattga ccaaatatcc ctgtagttcc tacggttctt gattttatct
      901 aaagatattt ttatcgtgga agctattgat caacttgaaa gaaaaacgtg tagctcatta
      961 tcactagttt atcgagtatg gatgaagcaa tggatcatcc tctcttaaat aaatttatta
     1021 aggatctgat ccttcaaatt ttttccatga tcgatgagca ggaacctact gaatctaaac
     1081 gacatcgggc acaaggaatt attaagattg ctaagcgaat gttttctata aagggtgttc
     1141 taagctatac agtgttaatt caaaagattc ccaatcatct tgtttataag aacaacgttg
     1201 aagattaaaa aagtgtttca atgctaaaat tgcaaaatac ttcaatgtta tgagacaaac
     1261 tgtctataag aatcaatttc cacccatttc atcacttgtt aaaagtttat gatccttttg
     1321 atttttatat taatttatct gtatttaata cagattttat ttattagttc atctgcctat
     1381 tgtattttat tttgttgcct taccatctga agtaaatata ctcttaattg tcaaattaag
     1441 ttagacagat catcaatact tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc
     1501 agtgattttc taggaagatt atttctgcgc cagcattttc taatgtttga atttgtgttt
     1561 tgtaatcttg gttgcttgtg ctaattcttg aatagccgta aattgccaaa atttcatcac
     1621 cctttctgga cataagtttc tgacaccagt taaaatcaag tctatcagta acccatacac
     1681 ccgtgaggac gggtgtattt ttaatccccc tagaaggggt cgacactcgc tgccccttaa
     1741 aaggggattt ttaaaagacc gccaactgct catcttcact cacttacttc tctttatttt
     1801 ttaaatggtt ctacgtactc tcttttactt ataatatatc gaatgcgatc atcagcttct
     1861 tgttcacgga tgtatttttc atttgtcttt tgattcaatc cgactgtact cacatagtat
     1921 ctcttcgacc aaaaggattt gtttccgtat ttatatttga gatttgcgtg ctgctcgtgg
     1981 attagtaccg cactacgacc tttcagatac cccataaaac tggatataca tatttttggt
     2041 ggtactcgaa caagcatgtg aatatgatct agcattgcat gtgcttctat gatttccaca
     2101 tctttcattt cgcacaactt cctaagtatt tttccaagat cttttcgtaa ttgcccatag
     2161 attacttttc gttgatattt tggtgtaaaa ataatatgat acttacaatt ccaacgagta
     2221 tatgctaaac ttttatcgtc atttgacatg aaaaaactcc tttcgatctg atgagcggtt
     2281 ggtagccact cactagtata tcgaaaggag tttttatcgc taaagatact tcttccacgc
     2341 gtagaaacgc gcggttttct tgtttcgcac gctttacgca caaaactgac tgaagtcatt
     2401 aagaaaataa aagggtctaa aacttttaag ttttgtaccg acccccaaaa gttagaccaa
     2461 aaaatctaac ttttggaggt cggtattttt tatggctaag tatacttttg aacttaaatt
     2521 aaagatcgtt catgattact tggatggtaa aggcggaagt gattatctgg caaagaaata
     2581 ttccattaag gctccatcac aagtgaagcg ctggatcaat gcctatcaag aatttggtga
     2641 agagggtcta gtaagaaagc gacaaaagaa aaaatattct gttcaattta agctggatgc
     2701 gatagagtta tatctaacaa gtgagctgtc ctatcgtgaa gttgccaaca cacttaacat
     2761 gaacaaccct aatttgatcg ctagttggat gcgtcaattc cgtgagggtg ggatcgatgg
     2821 actctcaaaa acgaaaggat gtccacctat cttgtctaag aaaaatgaac ccaaaaataa
     2881 gaaaaaatct acaactaaag caacgtctaa agaacgtgaa cgcatcgagg agttggagaa
     2941 acgagtccgt tcccttcaaa ttgaaaatgc ctttttaaaa gaattgagga agctgcgaaa
     3001 acaggaagcc caacaacgac gaatgaagca atcgcacgaa tcatcgcaag cctccgagga
     3061 ccgtttaaat tagttgagct tctagaaact ttaaagttcc ctaaggcaat ctttatgtac
     3121 tggcaaaaac gattggaccg taaaaatcca gatcaagaga ttgaagcgga aatgcttaaa
     3181 gttcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact aagaaaccgc
     3241 ggtttctcta tcaataaaaa gaaagttcag cgcctgatca agaagctagg aataaaagta
     3301 acgacataca cacgtaaatc acggcgctac aattcttatc gaggacaagt
//
LOCUS       DACDSR010000156         3329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDSR010000156 DACDSR010000000
VERSION     DACDSR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.160"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..290)
                     /locus_tag="IWK15_002727"
     CDS             complement(<1..290)
                     /locus_tag="IWK15_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9548250.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="IWK15_002728"
     CDS             complement(283..624)
                     /locus_tag="IWK15_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548251.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="IWK15_002729"
     CDS             complement(798..1523)
                     /locus_tag="IWK15_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9548252.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="IWK15_002730"
     CDS             1576..2052
                     /locus_tag="IWK15_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548253.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="IWK15_002731"
     CDS             complement(2058..2240)
                     /locus_tag="IWK15_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548254.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="IWK15_002732"
     CDS             complement(2240..2539)
                     /locus_tag="IWK15_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548255.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="IWK15_002733"
     CDS             complement(2644..2847)
                     /locus_tag="IWK15_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548256.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..>3329)
                     /locus_tag="IWK15_002734"
     CDS             complement(2862..>3329)
                     /locus_tag="IWK15_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9548257.1"
                     /translation="SEVLPTIRKTGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEY
                     EPKISYLDTILSSTDTVATSQIAADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMN
                     QGYTKSHTSEIPKADGGTKVVMNTKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT          905 a          668 c          508 g         1248 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcact
//
LOCUS       DACDSR010000157         3300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDSR010000157 DACDSR010000000
VERSION     DACDSR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.161"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..666
                     /locus_tag="IWK15_002735"
     CDS             <1..666
                     /locus_tag="IWK15_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9548258.1"
                     /translation="NEIFEMMDEEKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKF
                     LSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHE
                     LPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFL
                     AGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVR
                     IDK"
     gene            801..1379
                     /locus_tag="IWK15_002736"
     CDS             801..1379
                     /locus_tag="IWK15_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548259.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1325..1843
                     /locus_tag="IWK15_002737"
     CDS             1325..1843
                     /locus_tag="IWK15_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548260.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1851..2891
                     /locus_tag="IWK15_002738"
     CDS             1851..2891
                     /locus_tag="IWK15_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9548261.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            3140..>3300
                     /locus_tag="IWK15_002739"
     CDS             3140..>3300
                     /locus_tag="IWK15_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9548262.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1192 a          363 c          660 g         1085 t
ORIGIN      
        1 aatgaaatat tcgaaatgat ggatgaagaa aaaaaggtat cttatgataa acaatatata
       61 gaagatttat actacaaata tatggaaact gggaaaatac aggaggaata tatggaagaa
      121 ctgagaaaat ttctctcaga caaaaaaatt ctactgattg caccaggaaa aagttctgta
      181 gatgaggtag aaagaataaa agagtttgtt accaaagaag aagtcgtaat aattagtgta
      241 aattttgaat attctcatat agatatagat tacgtatttt taagtaattt aagacgtttt
      301 catgaattac cgaaagaaag cagaactaaa tgtattgtta catcaaatat acaagcatcg
      361 gatgtatatt tacggacgag ttataaaaag ttgttatctg aaagggaagt tgtaagagac
      421 aatgcaggat taatggcaat tagattttta gccgatatga atgtggatga aatattctta
      481 gctggatttg atggatattc acataatgaa gatgagaatt atggtgagca atccatggaa
      541 attataacaa aatttgcatt attagatgca atgaacgtgc aaatgagaga tgaattgtca
      601 gaattagcta aaaaagtaaa ggtgacattt ttaacggatc cacgttgggt tcgaattgat
      661 aagtagtagt aataatgttt ttaaaatttt gataaagaga tagtgagaga ataaaaatgg
      721 gtaaagtctt aaataaaaaa gaagaaaaat atttaaggga cgatggggcg agatgaaact
      781 ctaattttga acttttaaga atcatttcta tattgtgtat tgttgcttac catgctaatt
      841 tgcgtttaaa ttataatgca gacggaatta tagtacatgg ttcattaact gctaaccaga
      901 ttttcttgaa ttatattggt atttggggtc agctaggagt taattgtttt atatttattt
      961 cggcatattt tttaagtgag aaaagaaaca ttcactttga aaatgttttg ggaattgtat
     1021 tagaatgtat tttttatagt gtattagttt atggaattct ttgtgcatgt ggaatagaaa
     1081 cattttcatt gaacagcttt gtaaaagaaa tattatcatt tgctctaggt agatattggt
     1141 ttattacatc ctatttagca ttgtatattc taagtccgta tttaaatatc atgcttaaca
     1201 atctggaaaa acttcagttg caaaaaataa gtatgttatt gtttataggt gtatgctgtt
     1261 atcgtatgtt atttaaaaca tctttgtttg gggattgtgc tgttttaatt gcactatatg
     1321 tcatatgtta tacactgaaa aaaacgataa taatattttt gagagacaca gaatcatagg
     1381 aacaattgta tgtataacag ttataccact tctcatatcc ataggaatac ttttcaaatt
     1441 tataccaaat gcaatggtta accagatggt tcatattggt tctccattga tggttatagc
     1501 aagtatattt ttattttatg tatttaaaaa tttttcattt tacagtaagt atataaataa
     1561 acttgcgaga accagtttag gggtatatat tttacatgaa aatcattatt tcattgatgg
     1621 tatgaagtct agaggcttaa tatgggatgg gatatttgga ttaggcaaaa ggtttttaga
     1681 tagggatttt tggatttggt ttataattgc agttgtagtt gtgtgcataa tttgcatggc
     1741 tatcgatgtt gtaagagaaa aatttgaaag acagattttt aaaattaaga gtgtaaagaa
     1801 cctgtgtaaa cttatgaatc atgttataaa tatataccca taattaataa atgaggagaa
     1861 aagaagtgaa agttatatca ttcggagaat tattattgag acttgcagca cccggttatt
     1921 caaagttatt tcaaagagat agtttggaga ctacattctg cggaggggaa gctaatgtag
     1981 cggtatcctt agcaatattt ggatatcaat cagaatttat aacaaagctt ccggattcag
     2041 atgttggata tgcggcagct aatgctttgc gttattttgg agtaaatgtt gataatatta
     2101 tatttggaga gggacgcatg ggactgtatt atttagaaaa aggagcagct cagagaccat
     2161 ctaaagtaat ttatgacaga aaattttctg caatttcgtt agctgatagt gatgaattta
     2221 agtggaagga gatcctaaac gatgcaaatt ggtttcattg gacagggata aatccagcat
     2281 tgtctgagaa tctctttgag acgtccttag atgcatgtca ggctgccaaa aaaagaggta
     2341 taacaatttc ttgtgattta aattatcgat ccaagctttg gacatcagag gaagcaaaag
     2401 aaaagatgga acagatacta ccattggttg atatttgtat ttgtaatgaa gaagatgcag
     2461 aaaaagtgct aggaattaaa gctgagcata caaatattaa tagtggagaa ttaaataaag
     2521 atggctatgt gcaggtggct agggagattc acaagaagtt tggatgtagg tatgtagcta
     2581 caactctgcg tagaagttat tcggcaagta gaaatggttg gagtgcaatg ttatacgaag
     2641 cagaaaaaga taaagcgtat ttttccacag aatacaacat acaaattgtt gatcgcgttg
     2701 gtggtggaga cagcttttct gctggtataa tatatgcgtt atgtgaaaat atgtccggac
     2761 aaaaggctat tgaatttgcg actgcatcta gttgtttaaa acatactata gaaggtgatt
     2821 ttaatcgaac tacaattgag gatgtatata atttaatgaa tagcggtgga aatggtcgcg
     2881 tgttgcgtta gtcaacatat aaagataaat cacagtaatt tttatggtga ttatctgtct
     2941 tagtagtgat tagaataacg tgaaaataaa ttgttgagcg tttatactgt tttgaaaaat
     3001 aaatttaaca tattgaaaaa tacaaatata tacgtatatt ataaagcgcc gattgtaaaa
     3061 ttaagctaga caactaaaac agtcatttgt gctacactca aaatagttcc gaccaaagaa
     3121 ttataaggag tgagtgcaaa taacctatac ctatcttaca acggatgaac ttgtaataat
     3181 agagtcttat ttgaaaatta accaatctgt tgctaaaact gcgcattgct tgaatcgttc
     3241 aagtcaaacg atccataaag tatatctgtt cttcaagcaa agaaaatcag ccttagaata
//
LOCUS       DACDSR010000158         3283 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDSR010000158 DACDSR010000000
VERSION     DACDSR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.162"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWK15_002740"
     CDS             complement(<1..1097)
                     /locus_tag="IWK15_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9548263.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IWK15_002741"
     CDS             complement(1227..2603)
                     /locus_tag="IWK15_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548264.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="IWK15_002742"
     CDS             complement(2603..3259)
                     /locus_tag="IWK15_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9548265.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          998 a          617 c          443 g         1225 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tga
//
LOCUS       DACDSR010000159         3261 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDSR010000159 DACDSR010000000
VERSION     DACDSR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.163"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..889
                     /locus_tag="IWK15_002743"
     CDS             200..889
                     /locus_tag="IWK15_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9548266.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            903..2405
                     /locus_tag="IWK15_002744"
     CDS             903..2405
                     /locus_tag="IWK15_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9548267.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2418..2894
                     /locus_tag="IWK15_002745"
     CDS             2418..2894
                     /locus_tag="IWK15_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9548268.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2991..3101)
                     /locus_tag="IWK15_002746"
                     /pseudo
     CDS             complement(2991..3101)
                     /locus_tag="IWK15_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1082 a          442 c          621 g         1116 t
ORIGIN      
        1 aaatggataa ttttctttaa ttaaatagat ataactcata caaactcctc tacaatttca
       61 acttttctcc attatactcc aaaataataa aaaaaactcc taaatactcc atttttatct
      121 ttacttttat gaaagcggat gctatattgt ttttggagtt gttatccaaa aataataaaa
      181 aattggagtg tttaggataa tggataaaaa gaggttttta gatcttattt caaaaggttt
      241 gattgtttcc tgtcaagcat tacctggtga accattttac aaagaggaag aaagtttaat
      301 gccttatttt gctttggctg ctcaaagggc tggagcagtt ggtatacgtg caaattctgt
      361 tcgagatatt attgaaatta gaaagtgtgt atcattacca atgattggaa ttattaaaag
      421 agattatcca ccagaatctc cattcattac tgccactatg agagaagtag atgaattagt
      481 agaaaccggt gttgaggtaa tcgctcttga ttgcactctt agaaatcgac atgatggact
      541 atctataaat tcttttatta ctaaaataaa agaaaaatat cctcatcagt tatttatggc
      601 agatatttct acatttgaag aaggcaaaaa tgcatataaa gcaggaatag attttgtagg
      661 aactacttta agtggatata ctgaggaaag taaaaaacaa gatgggccag atatagattt
      721 gattaaaaaa ttggtagaag aagggatttg tgtaattgct gagggaagga ttcatactcc
      781 agaacaagca aaagagataa aaaaaatagg agttgcaggt attgttgtgg gtggtgctat
      841 tactcgacca caggaaattg catcaagatt tgttaaagca ttaagttagg gggagaaata
      901 aaatgtttaa aaaattttca caattaggac gcgctttcat gttaccaatt gctatattac
      961 cagtagccgg attactattg ggactgggag gagcgttaac aaatgagagt gctataaatg
     1021 cttatccatt tttagatcaa ccgtggttgc atacaatttt aagtattatg agttatgctg
     1081 gaaatgcagt ttttgcaaac ctagcactaa tttttgcaat tggtattgct gtaggacttg
     1141 caaatggaga taaaggaaca gctggtttag ctggtggagt agcttatcta gtatatactg
     1201 caactattag tgggtttctt gaactttttt cagcaaaaga tgcaacacta gatacaggtg
     1261 tagtgggatc aattgttgtt ggtggtactg ttgcattttt acacaatcgt tatagaaaaa
     1321 ttgaattacc acaattctta ggtttttttg gaggatcaag atttattccg attgtttcta
     1381 gtttagctgc aatcattatt ggttctttct tttatttaat ttggccacca attcaaaatg
     1441 gtttagtagt tgctggtgaa caaattgccc aaatgggtag tttaggaact tttttatatg
     1501 gttttttatt gcgtttaaca ggtgctgttg gactacacca caccatttac cctttgttct
     1561 ggtatacatc tttaggtggt accgaaactg tagctggatc aacaattgct ggagcccaaa
     1621 atattttctt tgcccaacta gctgatccaa accacactgg tctgtttact tatggtacta
     1681 gattttttgc aggaagattt gcaacaatga tgtttggatt accagcagca tgttatgcaa
     1741 tgtatcgtgc aattcctaag aaaaatcgta aaaagaatgg tgggctttat tttagcgggg
     1801 cccttacatc atttttaact ggaattactg aaccagttga atatatgttt ttatttgttg
     1861 ctccttggct ttatgtgatt catgcattct tagatggatt atctttttat tttgctgata
     1921 tcttaaatat tcgtgtaggg aattcctttt caggtggttt gatcgacttt ttattattcg
     1981 gtgtacttca agggaatgac aaaactaatt ggttaaaatt aattcctttt ggtattgcat
     2041 gggcatttat atatttcttt gtttttacat tctttatcaa aaaatttaag gtagctattc
     2101 ctggaatgga agaagaatca gaagttcatt ctgaaaaagt aataaacaaa aatacatcat
     2161 tacatgaaga gtctcttatc attattgaag ctcttggtgg aaaagaaaat attgagtctg
     2221 ttgaagcttg tgcaactcgt ttgcgtgttg ctgttacgaa tggggagatt attcaaaaac
     2281 aaacaataaa agacactggt gcaactgctg tcttcgaagt gaagggtggc atccaagctg
     2341 tttttggagg gaaagctgac ttattaagtc aagaaatcaa tcaaatatta ggaaaagata
     2401 attaggaggt ttttaatatg tttgaaattt tcaaaaagaa aaaacaagaa actttttatg
     2461 caccttgttc cggaaaaatc atttccatca cagaagtaaa tgatcctgtc ttttcacaaa
     2521 aattactagg tgatggtttt gccgtcatac ctgatggttc aaaaatttat gctccattgg
     2581 ctggtcgagt aacaaatata tttcctacta aacatgctat taatataaaa agcaactcag
     2641 gcattgatta tttgattcat attggaattg atacagttga attacaagga gagccttttt
     2701 cgatttgtgt tcaagaaaat gatgaggtta ctcctgaaac ctcgttggtt gatgtggatt
     2761 ttgacaaaat aataaaatct ggtaaagata cttctgttat agttgttttt ccggagaaaa
     2821 atcagttcaa tgatttaata atagataatg gtcgaattgc tcatgggaat tcatgtggaa
     2881 aaatcacttt ttaaaaaagt gtatataaaa agctataaaa taagctaaat aggatatttg
     2941 tcaaatgaag ttggtgaaca gaaagaactg ttttctttct gttcattttt ttattttatt
     3001 agtaatgatt ccccatgcca aatcatgatc cgtaatttaa agttataaaa gcttcgaaat
     3061 ccattggcat ttcgttggaa taccttgata tgattattca aatataaaat atagagagaa
     3121 gagagagagg gaagatattt ataaagggca aagttatgtt tactcaactt aggaaataga
     3181 aatagatcaa tttatattct cttgtcaatc gaatatgaac cttagcacta tataccaaat
     3241 atagtaaaat gctgaaagat g
//
LOCUS       DACDSR010000160         3227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDSR010000160 DACDSR010000000
VERSION     DACDSR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.164"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            396..1907
                     /gene="gadC"
                     /locus_tag="IWK15_002747"
     CDS             396..1907
                     /gene="gadC"
                     /locus_tag="IWK15_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9548269.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1920..>3227
                     /locus_tag="IWK15_002748"
     CDS             1920..>3227
                     /locus_tag="IWK15_002748"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9548270.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYV"
BASE COUNT          982 a          526 c          647 g         1072 t
ORIGIN      
        1 tttttctatt tacacaaaat attttacact ctcaacaaaa gtatcttcga ttaatacttg
       61 ctcaagttat gtgcagctac gaaaaagtag gtaccgaggt actttctttt tcgcagcttt
      121 ttttggactt atgagaagta cattcgctgg aagaatacga tataaaacga gctttttcac
      181 taaagatatt aatagagtga aagaaacaag cttttgttgt cagttttttc acttttctaa
      241 aaatgaaaat aaaaaattct ggaagtggag aagaattttg cttgctatac tttgtcttgt
      301 agaaaagagg tggacaaaat aatgcaagct caaataagcg attttccata accttggtag
      361 gaatgacaaa ttaaatagga aagggaagta ataatatgaa taaaaaaaag ttatctctat
      421 tcggtttttt tgccctgaca gcttccatgg ttttgactgt ttatgagtat ccgacctttg
      481 caacatcaca attacatttg gttttctttc ttcttttggg aggtatttta tggtttctac
      541 cagttgcttt atgtgctgca gaaatggcaa cagttgaagg atggaaagat ggtgggattt
      601 tcagttgggt tagtcaaact ttaggagaac gttttggttt tgcagctatt tttttccaat
      661 ggtttcaaat tacagtaggt tttattacaa tgatctactt catactagga gcgctgtcgt
      721 atgtaattaa ttttccagag cttaatacag atccatttat caagtttatt ggtgtattag
      781 tcattttctg ggtactgact ttttcacagt tgggcgggac caatcgtacc gctaaaattg
      841 ctaaagcagg atttgtcatt ggaattgtta ttccttcgat cattctcttt ggtttagcag
      901 cagcctactt tatcggcggt aatccgattc aaatggctat taatgcgaaa gcttttattc
      961 ctgacttttc tcagctttca actttagtag tatttgtatc ttttatattg gcttatatgg
     1021 gcgtagaagc ttcagcatca catattaatg agctggaaaa tccccaaaga aactacccat
     1081 tggcaatgtt tttactagtg attttggcca tcgccttaga tgcaatcggc ggtttctcag
     1141 tagcagcggt tattccacaa catgacttgt ctctcagtgc tggtgtaatc caaacatttg
     1201 aaacgcttat tttacatttt aacagtcatt tacattggtt ggtaaaagtg attgctctca
     1261 tgattgcctg tggcgttatg ggtgaagtta gttcttgggt agttggtcca tcacgaggga
     1321 tgtttgcagc tgctcagcga ggtctgttac cagttttctt ccggaaaact aataaacaag
     1381 acgttccagt tccgttgatt atgatacaag gaattatcgt tactatttgg gcagcagtct
     1441 taacttttgg tggaggaggc aacaatttat cattcttagt agcaatctcc ttaactgttg
     1501 tcatttatct cgtaggatat ttactatttt tcctaggtta ctttgtatta gtttttaaga
     1561 aaaaagactt aaaacgaact tacaatgtac caggaaaaac tgttggcaaa acgattatcg
     1621 gtggaattgg ttttatttta tcagtgttcg cattgcttat ctcttttgta ccaccagctt
     1681 caattgcagc tagtgagcac cgaacatacc aaatcatact acttattagc tttgtagtga
     1741 ctgttctttt accttttatc atttatgaag tacatgataa aagtgctcac acaactattg
     1801 aggaacctac tcacgtgaaa gctgaagacg ttaatcccgc tgtttatcca gctgctagag
     1861 gagagcacca cgttacaaaa aatgatgaac atattttaaa acattaggag gaattcatta
     1921 tgttatacgg aaaagataat caagaagaaa aaaactattt ggaaccaatt tttggctctg
     1981 caagtgagga tgttgacttg cctaaatata agttaaacaa agaatccatt gaaccacgaa
     2041 ttgcttatca attagtacaa gacgagatgt tggatgaagg aaatgcgcga ttaaacttag
     2101 ctactttttg tcaaacgtat atggaacctg aagcagtgaa attgatgacc caaacgttag
     2161 aaaaaaatgc aattgataaa tcagaatacc cacgaacaac ggaaattgaa aaccgctgtg
     2221 taaatatgat tgctgattta tggcatgctc caaataatga aaaattcatg ggaacttcaa
     2281 cgatcggctc ttcagaagcc tgcatgctgg gtggtatggc catgaaattt gcttggcgta
     2341 aacgtgctga aaaattaggt cttgatattc aagcaaaaaa acctaacctg gtgatctctt
     2401 ctggttacca agtttgttgg gaaaaattct gtgtatattg ggatgtggaa ctgagagaag
     2461 tcccaatgga tgaaaaacat atgtcaatta atctagatac tgtcatggat tatgtggatg
     2521 agtacacaat tggtattgta ggtattatgg gtattactta cactggtcgt tatgatgata
     2581 tcaagggtct gaatgattta gttgaagctc acaataaaca aactgactat aaagtataca
     2641 ttcatgttga cgctgcatcg ggtggctttt atgcaccatt tactgaacct gatctagttt
     2701 gggattttca attgaaaaat gttatctcaa ttaattcttc aggtcacaaa tatggtttgg
     2761 tatatccagg tgtgggttgg gtcttatggc gtgaccaaca atacttacca gaagaattag
     2821 tatttaaagt tagttactta ggtggagaaa tgccaactat ggctatcaac ttctctcata
     2881 gtgcagcaca actaattggg caatactaca actttgtacg ctatggcttt gatggttatc
     2941 gtgatattca ccaaagaact catgatgttg ctgtttattt agccaaagag atcgaaaaaa
     3001 ctggtatttt tgaaatcatt aatgatggat cagaattgcc agttgtgtgc tataagctga
     3061 aagaagatcc caatcgcgaa tggacactat atgatttatc tgatcgtctg ttaatgaagg
     3121 gatggcaagt cccagcctac ccattaccta aagacttgga tcaattaatt attcaacgct
     3181 tagttgttcg agcagacttt ggaatgaaca tggctggtga ttatgta
//
LOCUS       DACDSR010000161         3141 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDSR010000161 DACDSR010000000
VERSION     DACDSR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.165"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(358..972)
                     /locus_tag="IWK15_002749"
     CDS             complement(358..972)
                     /locus_tag="IWK15_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9548271.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1578..1955)
                     /locus_tag="IWK15_002750"
     CDS             complement(1578..1955)
                     /locus_tag="IWK15_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548272.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2255..3136)
                     /gene="galU"
                     /locus_tag="IWK15_002751"
     CDS             complement(2255..3136)
                     /gene="galU"
                     /locus_tag="IWK15_002751"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9548273.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          953 a          652 c          486 g         1050 t
ORIGIN      
        1 taacaaatgt actataatta ctttgtaaag agttttgggg aagactcttt catcaacgtg
       61 tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc cactactttt
      121 tatttttctg acactacata ttcttataac tcatttctca taactgtgaa acaacttata
      181 aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc ttaaaataac
      241 taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt tttttcaaac
      301 gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta
      361 ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa
      421 aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa
      481 gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg
      541 caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat
      601 taatggatga ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc
      661 ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc
      721 ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact
      781 tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct
      841 agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg
      901 aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata
      961 acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct
     1021 aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt
     1081 ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag caaataaaac
     1141 aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta gattaggtac
     1201 aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag ggtgtatgtg
     1261 tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc tttttttatt
     1321 cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat
     1381 acaaaaaaac attggtatga tttatatata tataaataac ataaaaaaag gaatggctta
     1441 tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa aaaagctttt
     1501 ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta tctttttttg
     1561 cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc gagagaatct
     1621 gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt tcttttcata
     1681 tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac gcaagcttcc
     1741 caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc tgcataccag
     1801 ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa tagactaata
     1861 agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt gagtatatca
     1921 agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct ttttttataa
     1981 ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc atttctctta
     2041 tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat aatattttct
     2101 gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac tgatctagag
     2161 tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat
     2221 agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt agctttttac
     2281 tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga agtccatatt
     2341 caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt cctttaaact
     2401 ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt agctgtatct
     2461 cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga gtcagtagat
     2521 aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt tctacaaacc
     2581 cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt ccgtatttag
     2641 atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt tcttcataat
     2701 ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc cccagcatga
     2761 cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc cccagtcctc
     2821 ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca actaaagcga
     2881 gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt
     2941 gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg attcccgaat
     3001 ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga agcatttctt
     3061 ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca ggaatgactg
     3121 cttttttgac cttcacttgc t
//
LOCUS       DACDSR010000162         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDSR010000162 DACDSR010000000
VERSION     DACDSR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.166"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWK15_002752"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWK15_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWK15_002753"
     CDS             1360..1992
                     /locus_tag="IWK15_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9548274.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWK15_002754"
     CDS             2197..2424
                     /locus_tag="IWK15_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9548275.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWK15_002755"
     CDS             2493..2759
                     /locus_tag="IWK15_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9548276.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWK15_002756"
     CDS             2749..3018
                     /locus_tag="IWK15_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9548277.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDSR010000163         3118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDSR010000163 DACDSR010000000
VERSION     DACDSR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.167"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(553..2166)
                     /locus_tag="IWK15_002757"
     CDS             complement(553..2166)
                     /locus_tag="IWK15_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9548278.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..>3118)
                     /locus_tag="IWK15_002758"
     CDS             complement(2307..>3118)
                     /locus_tag="IWK15_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548279.1"
                     /translation="FRLLINESANLYAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKE
                     TQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKPAISYDQ
                     EINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKH
                     ILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKEL
                     KMEKAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1006 a          544 c          544 g         1024 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaat
//
LOCUS       DACDSR010000164         3012 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDSR010000164 DACDSR010000000
VERSION     DACDSR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.168"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWK15_002759"
     CDS             177..359
                     /locus_tag="IWK15_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9548280.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWK15_002760"
     CDS             462..746
                     /locus_tag="IWK15_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548281.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWK15_002761"
     CDS             762..998
                     /locus_tag="IWK15_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548282.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWK15_002762"
     CDS             1020..1274
                     /locus_tag="IWK15_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548283.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWK15_002763"
     CDS             1356..1541
                     /locus_tag="IWK15_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9548284.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWK15_002764"
     CDS             1608..2003
                     /locus_tag="IWK15_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9548285.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWK15_002765"
     CDS             1993..2370
                     /locus_tag="IWK15_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548286.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWK15_002766"
     CDS             2371..2766
                     /locus_tag="IWK15_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548287.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DACDSR010000165         3010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDSR010000165 DACDSR010000000
VERSION     DACDSR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.169"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            624..>3010
                     /gene="esp"
                     /locus_tag="IWK15_002767"
     CDS             624..>3010
                     /gene="esp"
                     /locus_tag="IWK15_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9548288.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT         1097 a          456 c          591 g          866 t
ORIGIN      
        1 caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga
       61 aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg
      121 agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat
      181 gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat
      241 ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt
      301 attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg
      361 cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt
      421 tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact
      481 ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag
      541 attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat
      601 tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa
      661 aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg
      721 ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa
      781 tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag
      841 cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt
      901 tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg
      961 aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat
     1021 tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg
     1081 gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt
     1141 ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata
     1201 cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag
     1261 agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc
     1321 atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt
     1381 cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg
     1441 gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca
     1501 caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat
     1561 attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag
     1621 ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg
     1681 tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg
     1741 aaaattatgg cataggttca ataactaata atgatttaac taaacttatc gattttgcta
     1801 atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg
     1861 aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag
     1921 atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg
     1981 gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag
     2041 agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg
     2101 acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc
     2161 aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag
     2221 atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat
     2281 taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag
     2341 aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg
     2401 gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag
     2461 agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg
     2521 taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt
     2581 ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag
     2641 ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt
     2701 caatttcaga tgtctataat aagtacttcg gtaatgcaag tgttgatgca aacactggta
     2761 ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc
     2821 ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa
     2881 gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct
     2941 tcaccgcagg ggaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
     3001 gcgtaagctt
//
LOCUS       DACDSR010000166         2947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDSR010000166 DACDSR010000000
VERSION     DACDSR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.171"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(449..865)
                     /locus_tag="IWK15_002768"
     CDS             complement(449..865)
                     /locus_tag="IWK15_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548289.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IWK15_002769"
     CDS             complement(867..1148)
                     /locus_tag="IWK15_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548290.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IWK15_002770"
     CDS             1948..2922
                     /locus_tag="IWK15_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9548291.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACDSR010000167         2925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDSR010000167 DACDSR010000000
VERSION     DACDSR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.172"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..443)
                     /locus_tag="IWK15_002771"
     CDS             complement(<1..443)
                     /locus_tag="IWK15_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9548292.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="IWK15_002772"
     CDS             513..863
                     /locus_tag="IWK15_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548293.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="IWK15_002773"
     CDS             1277..2095
                     /locus_tag="IWK15_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9548294.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="IWK15_002774"
     CDS             complement(2168..2527)
                     /locus_tag="IWK15_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9548295.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          934 a          442 c          537 g         1012 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaat
//
LOCUS       DACDSR010000168         2870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDSR010000168 DACDSR010000000
VERSION     DACDSR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.173"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            803..1069
                     /locus_tag="IWK15_002775"
     CDS             803..1069
                     /locus_tag="IWK15_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548296.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1127..1639
                     /locus_tag="IWK15_002776"
     CDS             1127..1639
                     /locus_tag="IWK15_002776"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9548297.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1636..2673
                     /locus_tag="IWK15_002777"
     CDS             1636..2673
                     /locus_tag="IWK15_002777"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9548298.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1037 a          506 c          571 g          756 t
ORIGIN      
        1 ttttattgat gaccacgaaa gtgcatggcg atatttgatg ttgatacttt atgcagtacc
       61 ctatgtattc ttcgctatca ttctgtggct catagcattg ttttttagag tatttaatga
      121 cctttaggta gatttatata taaaaagggc taaatacgca aaataccccc ttaaaatgca
      181 aaataaatgc aaataagggg gtaactcctt atgaaaaaac aactaatagt attataacac
      241 gtttttatgc cgagaaaact tattggttgg aatgggctat gtgttagcta acttgttagc
      301 gagttggttg gacttgaatt gggattaatc ccaagaaagt accaactcaa caacacataa
      361 agccctgtag gttccgacca ataaggaaat tggaataaag caacgaaagg agttgagtaa
      421 aatgagtgag aaaacgaaga aaatcattcg agtatcatta gctttttcac aagtagtagt
      481 gcaatcattg atagtttatt atacttacca gcaatataaa ttatctaaag agaattaagt
      541 tttgattatg taataagttt tggtactaaa aaagacttga tctaattaga ccaagtcttt
      601 tgttagtgtt atattaataa caaaataaaa ataagaagtc gctcactccc cgaccaaagt
      661 ttgtgagcaa aataaacagt cgatgtttgt ttcttaattg tatatcttga tattaaacga
      721 tttaagatta ttcttcaaga tatatattcg caaatgtaat tggaaaagcg acttcttaaa
      781 caaaattaag gagtcgcttt ttatggcaaa aataacgaaa aatgagcaag aaaatttggt
      841 ggaaaattac acaaaaaaac cggcgtactc taatagccgg ttagacgcac atttcgtgtg
      901 cacttctaat ccgaaattga gctttgacgc tatgacgata gtcggaaacc ttaataaaaa
      961 cagcgctaaa aagctatccg attttatgag tttagatcca caaattagac tttgggatat
     1021 acttcaaaca aaatttaaag ctaaagcact acaagacccc ctatgttagg ctagttgtcg
     1081 tataggataa taagttataa taaagactat acggaggatg attaccatga caaataaaaa
     1141 caatgaacgt tactctaccg aactgaaagc gacagccatc aagaaaatga tgccacccga
     1201 aaataggggg attcgccaat taagtcaaac tttgggcatt ccagaagcga ctctttattc
     1261 ttggcgtaaa aaagctagaa ttcaagggaa tactacaccc ggaacgaaaa aaaatgtcga
     1321 aaaatggtca agtgaagata aatttttaat tgtgatggaa tcattctcaa tgaaccaagt
     1381 agaacttgcg gaatattgcc gaaagaaggg cttatttaaa gaacaaattg aggcttggaa
     1441 gaaaacgtgc ttatctgcga atgaacagga agaaaatcga acgagagaac ttgcaactga
     1501 actaaaagaa gagaaaaaac aagcgcgtca attagagaaa gaattacgtc ataaggaaaa
     1561 agcattagcg gaagccgctg ctctcttatt actaagaaaa aaagcacacg cgatttgggg
     1621 ggaccaagag gaagaatgat ttcatcttca gatcgcaaac aagctgttga acttattcaa
     1681 gaagccaatc gcaatggggc ccgtctcacc tatgcctgta atgagctgaa tattagcgtt
     1741 cgaacctacg aacgatggac gcgtgaaggt acgatagcgc atgatcaacg ccctttggcg
     1801 aaacgtcctg tacccaaaaa taaactgacg gatcaagaac gcgaaaaaat aatagaaacc
     1861 gtttcaaaaa aagaatttat gaatttgcca cctagccaaa ttgtaccaaa acttgcggat
     1921 tgctcgattt atattgcatc agagtctagc ttctatcgtg tattgcgaga aaaaaatatg
     1981 caacatcacc gtggccgaag tcaagtttca caaaaacgca taccgccaag tcaccttgcg
     2041 acgaagccaa atgaagtttg gacttgggat attacttggc taaaaggtcc aataaaagga
     2101 ctcttttacc ggttatatct catcattgat ttatttagcc gtaaaatggt aggctgggag
     2161 atttgggaat cagaagaagc tcgtcatgcc gaaaaactta ttaaacagac ggtgcttaag
     2221 gaacgtatcc aaggacagcc gttggtttta cactccgata atggcagccc tatgaaagcc
     2281 gcgacttttc aagtcttact cgaaaaatta ggaattcaaa gttcttattc aagaccacga
     2341 gtgagtaatg acaatcctta ttcggaagcg gctttccgaa cgttaaaata tcgaccagaa
     2401 tatcccaata acggctttaa aacaatagat gatgctcgaa aatggacgaa agagtttgtc
     2461 gattggtata acgattctca tttgcatagt gggattcaat ttgtgtctcc tgcagcgtgt
     2521 catgaaggac gacatctcgc tattttagag aaacgaaagc aggtttacga ggacgctaaa
     2581 aagcgccatc ctgaacgctg gtcaaaaggt gttcgaaatt ggactccaca tcaatcggtg
     2641 gcgttgaatc ctttaaaaga aacagaaagt taacaggagc ataatctttc aagattatca
     2701 aataaccctc gtcacccaat aggggaaaaa gttattcttc ccctattgga taacgaggcc
     2761 aaccgaccat agggaggttc tgctttttga taggtggaaa ataaaaatac gacaactata
     2821 ttgacaaaca ccgaccatcg tccagtaaag agacgcaata aattctatcg
//
LOCUS       DACDSR010000169         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDSR010000169 DACDSR010000000
VERSION     DACDSR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.174"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IWK15_002778"
     CDS             562..843
                     /locus_tag="IWK15_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548299.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IWK15_002779"
     CDS             845..1261
                     /locus_tag="IWK15_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548300.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IWK15_002780"
     CDS             complement(1422..1688)
                     /locus_tag="IWK15_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548301.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IWK15_002781"
     CDS             complement(1646..2620)
                     /locus_tag="IWK15_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9548302.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          929 a          614 c          396 g          918 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tctttcttgt tccaattttt
     2041 tgttatacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaaag aaacaggatc aaccacccga tattgggtaa
     2161 tgaaatcatc tggcacatca ataatgtcac aagcaatatc tgcacgtgag aaatgtggtt
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc ccttttccct cttgaaactt aagaatctca atataagcta
     2341 tattttcctc atgttggtct agtaattgcc agccattatt tccaaccgct tttaaatatc
     2401 cgttaccttc gtttagacgc ataagctgtt caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACDSR010000170         2781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDSR010000170 DACDSR010000000
VERSION     DACDSR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.175"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWK15_002782"
     CDS             174..518
                     /locus_tag="IWK15_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9548303.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWK15_002783"
     CDS             519..938
                     /locus_tag="IWK15_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9548304.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWK15_002784"
     CDS             complement(1030..1380)
                     /locus_tag="IWK15_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548305.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWK15_002785"
     CDS             complement(1346..2566)
                     /locus_tag="IWK15_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9548306.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1034 a          499 c          535 g          713 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt a
//
LOCUS       DACDSR010000171         2774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDSR010000171 DACDSR010000000
VERSION     DACDSR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.176"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..590)
                     /locus_tag="IWK15_002786"
     CDS             complement(<1..590)
                     /locus_tag="IWK15_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548307.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYL"
     gene            complement(1304..1594)
                     /locus_tag="IWK15_002787"
     CDS             complement(1304..1594)
                     /locus_tag="IWK15_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548308.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1802..2494)
                     /locus_tag="IWK15_002788"
     CDS             complement(1802..2494)
                     /locus_tag="IWK15_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9548309.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          842 a          507 c          387 g         1038 t
ORIGIN      
        1 atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac ttgttcttga
       61 ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt tatttcttct
      121 cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac tggccactga
      181 tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg tattaaacag
      241 tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac atacttgcaa
      301 agtaatacta caattcctaa cataatcgta gaatttaata atttccatag cataaagtgt
      361 ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt cttataatgt
      421 tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac tacgtcatcc
      481 gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat ccctattagt
      541 aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat ttttatactc
      601 cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa gataacttaa
      661 aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag aaatctacga
      721 tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
      781 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
      841 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
      901 gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt ttacgactat
      961 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
     1021 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
     1081 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
     1141 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
     1201 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca tccatctttc
     1261 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     1321 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     1381 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     1441 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     1501 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     1561 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     1621 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     1681 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     1741 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     1801 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     1861 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     1921 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     1981 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     2041 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     2101 tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat
     2161 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     2221 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     2281 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaag cgcttgctct ttatctgtca
     2341 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     2401 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     2461 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     2521 ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatgtgt
     2581 aattttaata taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa
     2641 atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac
     2701 aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt
     2761 tattacaaaa aacg
//
LOCUS       DACDSR010000172         2730 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDSR010000172 DACDSR010000000
VERSION     DACDSR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.177"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(3..188)
                     /locus_tag="IWK15_002789"
     CDS             complement(3..188)
                     /locus_tag="IWK15_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548310.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            474..848
                     /locus_tag="IWK15_002790"
     CDS             474..848
                     /locus_tag="IWK15_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548311.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            853..1281
                     /locus_tag="IWK15_002791"
     CDS             853..1281
                     /locus_tag="IWK15_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9548312.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1372..2286
                     /locus_tag="IWK15_002792"
     CDS             1372..2286
                     /locus_tag="IWK15_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9548313.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            2402..>2730
                     /locus_tag="IWK15_002793"
     CDS             2402..>2730
                     /locus_tag="IWK15_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9548314.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETY"
BASE COUNT         1020 a          439 c          454 g          817 t
ORIGIN      
        1 ccctatcgga tgtccaatat ttttttcact gtttcgatat gctgttgtgc tttctttcca
       61 tcacgattgc cgtttagaat atctgataaa taagctcctg aaataccgat aagcgcagct
      121 agttctttga aagtcattct tctttttctc atctccgctc gaatttttaa gtctaaattc
      181 tcagacataa aaatagctcc tttctaaaaa aataatctgt aagctaaaaa attagctaat
      241 attcgttgac aataactaat atttttatta gtatatagac atagctaaat aagacacaga
      301 aatgcctagt aatttacatt tcagagtttg ccgaccgtga agtgtttatt agttatcaag
      361 tctttttagc taatatttta gcttacgaaa acagtatact aacactttta ttagttgtca
      421 acacctttaa ctaatttttt tattagtgtt ttcgtagctt gtgaggtgcg aaaatgaatt
      481 tactagatag aataaaggaa ttagctcata aaagaggcat aagtataact caattggaag
      541 aagaattaaa tatacctaaa aacacgattt atcaatggaa aaatagaact cctagtacta
      601 aaagactaca acttgtagca gattatttta atgtaactac tgattattta ctcggtagaa
      661 accaagttcc tgactgggca acaaaagatg aagtagttga acttgataaa ttactagatt
      721 caaatgtcaa tatgtcttat ggcggagaaa cactgacgcc tgaacaggtc caacgcgtaa
      781 aagatatcct tattggaacc ttctgggata ttgtgaaaga agacaaagaa aaaggcaaaa
      841 agatgtgagc ttatggagat ggatacgatt aatttagtcg aggagttgaa gcggaaatac
      901 cagtccgcta atccttttta tatttgtgaa aagatgggca ttaaaattca atacgttcct
      961 tttatcgaga atcccaaagg acagtttcaa gaaattagag gtcgtgcaat aatcttctta
     1021 aatgatgaac tgagagactc tgaggaaaga ttctacattt gcgctcacga attaggtcac
     1081 gctatttttc atcgtggctt atccagttac tatgtatcaa caagaacatc tagaagcaaa
     1141 tctgaaagcg aagctaattg ctttgctgct aatctcattg tttctctata caaagaagac
     1201 aatgatcaat atcctaaacg aattgaggaa ttaaagaatc tttacggact tccagaaagc
     1261 gcttatcgct ttcttatata atgtaaaaaa agtccgtgct gcaacacgga ctaaaaacct
     1321 tatttctaag atcctacaaa aaaatcatat catagaagtg aggaattaaa aatgaaaaaa
     1381 attgtttttg ggttgctttt tttaggcttg gtattaagcg gatgctccac tagtcctaat
     1441 tctgctagca ctaaagaaaa tactgtttca tcaacatctg aatatgaaac aaaaaaacta
     1501 acttcatcag acgttcataa aataaaagtt ggtgatgatt taactcaagt ttatcagaca
     1561 cttggttcac cgttacgtaa atggacaaat gattttgttt acaaagaatt agacaatgct
     1621 atcacacggg atgaattaat tgtatctatt tcaaaggatg cacaaggaga ttattataaa
     1681 gagcactctg aattaattga acatggaaat agtgcaaaag atataaaaaa attagagatg
     1741 ctccaatata cttattcaac tgattctgat gatgaaacaa tgttaatttg ggttagtcca
     1801 aaaactgaac aggttgtata ttataatcaa cgaactttct tagataaaga tggaaaatcc
     1861 aaagattcaa gctctactac aactacaact acaacttcgt cttcagaacc ccagacggca
     1921 tcagttgcac atagtatagg agaagcagtt ccttttactt ctgaaaatgg taactctatt
     1981 aacgtaactg tccagtctgt atcaaaagat tacggtgatg atttctataa accagaaggc
     2041 ctattttacg caaaggtaga ttttcttgta gaaaacacag ggactacacc ttttgatgca
     2101 agttcaagtt atttagaatt ttatgattca tcagatatta aatctgattt agctagtaga
     2161 gattactttt cggaaaccat ccaaccgggt aaatccgcac aaggaacagc atattttgaa
     2221 gtgaaaaatg atggaacaac atttgaagtg ttcttcgcag atacttcatg gaaaggtagc
     2281 tactaaacaa aaaaacacgc cccctccctc gccaaagttt gtggacgtga tgaaaaataa
     2341 acctgctata attggcttac ttatccattc ctattatagc aacaaatagg agatgaaaac
     2401 aatgtggatt gaaaccaaaa ctgataaaaa cggaaaaaaa gtatataaat ataatgagcg
     2461 atatattgat ccaaaaacta gaaaaagaaa aaaagtgagc attacttata aaaataaatc
     2521 tcgagaaact cagaaagtgg cattgctaga gttaaataaa aaaattgata taaaattaaa
     2581 cgaaaaaaca cttcagaagc ctgatctaac atttcatgag cttgtcgaag aatggttagt
     2641 tatttacaaa agacaagtta aggagtctac atattatcct acgaataata tcttaaacac
     2701 tataaagaaa aagatacctg agacttacat
//
LOCUS       DACDSR010000173         2727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDSR010000173 DACDSR010000000
VERSION     DACDSR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.178"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..369
                     /locus_tag="IWK15_002794"
     CDS             7..369
                     /locus_tag="IWK15_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548315.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            489..679
                     /locus_tag="IWK15_002795"
                     /pseudo
     CDS             489..679
                     /locus_tag="IWK15_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1456..1998
                     /locus_tag="IWK15_002796"
     CDS             1456..1998
                     /locus_tag="IWK15_002796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548316.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            2447..>2727
                     /locus_tag="IWK15_002797"
     CDS             2447..>2727
                     /locus_tag="IWK15_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9548317.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
BASE COUNT          951 a          407 c          614 g          755 t
ORIGIN      
        1 tagtttatgg gaatgacttt agcagatatt aaagcgcaag aagaaaaatt aaaaaaagaa
       61 caagcgaaac ttcgccaaca gaaaaaacgc ttgattgaga aagaaaatgc tgcaatgggc
      121 aaggctcttc ggaagcgttt tgggtttgaa acttttgctg attttgagcg gtggctatcg
      181 tcgatttctg acgatcagca acacgaaaaa tcagtggata atgagtggct tgctgatgaa
      241 aaatcagcgc caagcgaacg agattatccc gatgaatctg acggcttatt cggagtgata
      301 ccgaataaga aaagtcagat gagtgagagt aaatttacgt caatatttaa tgacgtgaat
      361 ttacgataag cgattatttt gcgtgaagca aaataaagcg taaaaggagt tgagagcgtg
      421 acacgctttc ggctcctttt ttcgttgtca cttgtgacag cgaataccct cggagagcat
      481 ttggaattgt gacagccatg tcacaattag gaaaatgtta ctctttgaca aagagtaaca
      541 gaaaggttta cactcttttt attagcgaca gccgaaggag ggttttaact tggggttcag
      601 tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc gtgagttaac
      661 tgatagccaa aaaatcaatg actggcataa gcatattgat ttcacaaaat cggatgaaaa
      721 tatctattta gaacaaacaa atattcgtga aaaatatgaa gaattattcg gtgaagccgt
      781 agaaaaatat aatgcaaagc agaaaagagc cgatagaaaa atcgaggact atttagcaaa
      841 ggttcgcaag gataaaaaat tggaaccaca gcgagaattt atcgtgcaaa ttggtactct
      901 tgatgatttt cgtactactc gtgatgatgg ttcttctact ggcatatcgg aacaacaagc
      961 agaacaaaat cgagtgattg ccaacaaaat tttagttgaa tatttcaaag aatttaaaga
     1021 acggaatcct aatttatccg tctacaatgc tgttattcat aatgacgaag taagtccgca
     1081 cctgcatttg aatattgttc ctgttgctga agggtataaa cggggtgttc aaaagcaacc
     1141 aagttttaac aaagccttgc aacagcaggg tttacgagca gataaagaga atggtcgagt
     1201 gatttggaat aattttagga atcaggaagt tgagtcaatt gaacgtttga tggcagattt
     1261 tggttgggag cgtgagttag tcggtactaa caaaatcaaa gatgttcacg aatataaaga
     1321 aatcatgagc gagatttcag aattgcgtca aacggccgta aaagagcgtg agagcgtttc
     1381 tgatgagttg aggtatattt ttctaggcaa agagaagaaa atgagcgaga aaaggctctg
     1441 gtggcttctg agagagtgaa gattgaagaa gagaagaaaa atatgctgaa tgtaaaaaaa
     1501 gagatggcgg tcattttcca aaaagatatt gttcctttag aaagtttcaa gaggcttcct
     1561 gatggttcgt atcatttatc tgggaatgat tttgttgatt tgtatcaacg agctacgaga
     1621 gcaacaaatg ccaagagaaa tgctgataac atgagtgagc aatatttaca gatgcttgag
     1681 caaaatacag aattaaatga gtatgtagct acattggaag aagagcgtca tttgtctggt
     1741 agaaaacaat tagcagattt acaagtggaa aataaaaagc tgaaagaaga aaatcgctct
     1801 ttacgccagc gtttagagcg attaaaaaac gcatttcaaa aatcgatcac tcgtttgtct
     1861 atccgttttg gtcttgcaga aaaggatatg gggctttctg aggaattaga agtgttagag
     1921 caggagaaaa ccttatttta tcgtcaaaac gtctcacagc gtaaattaga gcgttctgaa
     1981 gatgagatcg agtggtagtt acgatgtgca gtcaacggta ccggaggcgg tttgtgccga
     2041 ggataaggag ttgacggcta aaccactaat ccctgaagct gacatttgct aaaaaatggc
     2101 aagtccattt tttggccaac ctagcgttag cgaagtttgg gggcgccctt ttcaaagggt
     2161 gcggttttat tttttggata tttgcatagc taaagagaag tgctattatt gagttatcaa
     2221 ataaataaac tgaaaaaaga gcaaaaaaat aaagtcgagc aacgctggac acgttctcga
     2281 cctttacgga aactaatcgc actagctccg taacttctta atcattatac gaatattttt
     2341 agatttcttc aagactagag attttttggt aatgaatttt taagagttac gtgaactttt
     2401 gaaaattctt tccagaagtc tctttttctt tgaggaggaa caaaatttga gtaaaaaagg
     2461 cacgatcata aatgagtggg aaaaattaac agccccctct aacaggcggt taaagtatca
     2521 aagtttgacc cttcccttaa aaatgcagtg gtcaattgac cgaattacca tagtcggaaa
     2581 actgaaagaa aatatttatt atcacactcc aaatgatgtg ttgattttaa attttgaaca
     2641 gcttatgcgc ctaaatgaag gtaatggtta tttaagatct gttagtaata atggttggca
     2701 gttgttagac caacatgaag agaatat
//
LOCUS       DACDSR010000174         2542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDSR010000174 DACDSR010000000
VERSION     DACDSR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.179"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(128..742)
                     /locus_tag="IWK15_002798"
     CDS             complement(128..742)
                     /locus_tag="IWK15_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9548318.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            980..1402
                     /locus_tag="IWK15_002799"
     CDS             980..1402
                     /locus_tag="IWK15_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548319.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1412..1615
                     /locus_tag="IWK15_002800"
     CDS             1412..1615
                     /locus_tag="IWK15_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548320.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1826..2410
                     /locus_tag="IWK15_002801"
     CDS             1826..2410
                     /locus_tag="IWK15_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9548321.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          837 a          390 c          455 g          860 t
ORIGIN      
        1 ttagttaagt aaaaaatggt ttgttggaat tttatgcttt atctgttctg agtaagactt
       61 aattttatag atagtaatta tatgtaggaa attaaagatg agaagataat aaaagcagca
      121 tgatcaacta tttgtcattg accatgctgt ctttttttat tctatacacg gtttgtctcg
      181 taacattgta gtctttagca atcttagcaa tcgcaacacc atttttaaga tcttcaacga
      241 tgcttttgta aacaaggcga cgttggggat cttttgcgtc tgcactgtat aatttgggcc
      301 gccctttgta gacgccgttt gcttttgcga tcttaattcc ttgtgcctgg cgacgtttcg
      361 attcagtccg ttcttgttca gcgatcatgg ccaaaatttg gatgatcaga tccttaatga
      421 atttatccag gagaggattg ccaattgctt cagccatgat cggtaaacta gtaatgatca
      481 gcttcacatt tttcttcttc aagtaattga ctgaagcgat gatctcgtca taatttcttc
      541 caagtcgatc aatagcttcc acaataaaaa tatcttgatc tcggacgtaa tctaatgctt
      601 cttggaaaac aggtcgatga gtaatcgttg cgccagattg tttctcgatg aaaattctct
      661 ctgcaccgaa ttttttcatt tcttcaatct ggcgttgttc attttgatcg atcgatgaga
      721 cacgtatgta tgcaactctc aaaactttta cctactttct gcaactattt aaaatacacc
      781 ctaatcatac acgaggaagc catatgtcac ttttcttttt aaatggcaat attttttgta
      841 aataaatgtc aatcaagaaa atttaaacga ttatgacatt aaaaatatat tttatgaatt
      901 ttctagtata taacttgatt tatctgttaa gatttattaa gaaaacgttt tcttagtttt
      961 gatttcgagg tgatagacaa tgaaaaataa gtttatttta tctttaggta ttttaactat
     1021 tggaattagt gtaactggaa ctacaattcc tacatttgct gatagtacaa atgaaccaac
     1081 tactgtagta gataataacc aagaattaac tcctttagat agggacggaa tagccattgg
     1141 taacccaact aacgacttaa ctgttactga aaattattta atagtttctc cattacttgc
     1201 tctagcagct tctaaaacga tacgagtgac aggaacgatg atttttgata aagttggttg
     1261 gccttctaga cctcctcaat cacggaattg gaaagaaaaa agaaatggtt attggtacta
     1321 tggaaaattg tatttacaaa gttataaatc agctggaaac aattggattg cgacttattc
     1381 aggaacctta actagaaagt agggattttt tatgccagaa tttgtgaaca caccaaattt
     1441 ttatcaagat agcgatattc taagtaaaat taatttaccg aaagaagaat cagctttaca
     1501 agttcagcga actttacagg agacacaaaa aattcaaaaa tcaatttcta atctagaaga
     1561 ttctgaagaa aaaagaaaaa aaagagacca aaaaacatat caaaaagaaa aataatgatt
     1621 ttaaaaaaag agtgtctagt cgacattctt tttttctaat tgaaagtgtt cataaaatca
     1681 cccatttctg gacactttct ttttttgctt tatttttcaa aaaaacaatt catttcatgt
     1741 tcataaaccc ataacaatat ctatgttcag ttaaactata tggataaagt tatgttacaa
     1801 taaaattaat gaaggagtga tttttttgaa aataggatac gcccgggtat ctactggttt
     1861 acaaaatttg gatttacaag aagacagcct aaataaattt ggttgtgaaa aaatttttac
     1921 tgatcatatg agtggggcaa aaagtaatcg accaggatta gaaatggcca ttgattttgt
     1981 tcgctctggc gatacacttg tcgtttggcg cttagatcgt ttaggtagaa atatggaaga
     2041 tttgatttca atcgtcaatc gactgaatga gcgtggtgtg agttttcata gtattcaaga
     2101 aaatattacg atggacaaat caagttctac aggacagctc atgtttcatt tgtttgcggc
     2161 atttgcagaa tttgagcgta atttgatcct ggaacgatcc gcagcaggac gagcagctgc
     2221 tcgtgcaaga ggacggtttg gtggtcgtcc tgaaaaattg tctgaacaag atttggaatt
     2281 actgaagact ttagtggata gcggtacacc tattaagact attgcggaac ggtggaacgt
     2341 ctcaagaacc accatttatc gttatttggc aaaaatggag gagaaaaatg gaaagacaaa
     2401 ttctcattag tttttgtcat ttaatgctga aaatttttgc catttatcta gaaaagtgac
     2461 aatagtatga gaattttttg taagtgtcta ctgataactt attctcaaat gttgtaagga
     2521 ttttattcat ttgttaatat ta
//
LOCUS       DACDSR010000175         2535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDSR010000175 DACDSR010000000
VERSION     DACDSR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.180"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..236
                     /locus_tag="IWK15_002802"
     CDS             <1..236
                     /locus_tag="IWK15_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548322.1"
                     /translation="KQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQEVWINT
                     ELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            267..446
                     /locus_tag="IWK15_002803"
     CDS             267..446
                     /locus_tag="IWK15_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548323.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(489..959)
                     /locus_tag="IWK15_002804"
     CDS             complement(489..959)
                     /locus_tag="IWK15_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548324.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1046..1744
                     /locus_tag="IWK15_002805"
     CDS             1046..1744
                     /locus_tag="IWK15_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9548325.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1922..2263
                     /locus_tag="IWK15_002806"
     CDS             1922..2263
                     /locus_tag="IWK15_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548326.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2256..>2535
                     /locus_tag="IWK15_002807"
     CDS             2256..>2535
                     /locus_tag="IWK15_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9548327.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGH"
BASE COUNT          929 a          367 c          506 g          733 t
ORIGIN      
        1 aaaaacaaga ttttgtaaaa aaatacaatt attctccatc tacttatcaa cgtcgaatgt
       61 cggaactaaa aaatacagca attttctcag cggcgtatga acgggtcaca ggacaagaag
      121 tttggatcaa tacagaatta tacgataaat ttttgtcttt caaatcctat aacaggttac
      181 gcacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat ttataaaaaa
      241 cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcattg gtattcagcg
      301 caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg ataggaacga
      361 ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag tacagagcga
      421 aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag aatgtatatt
      481 actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt atttttgata
      541 tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt ttggtgatta
      601 aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga tatagcactt
      661 ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc gactgatcct
      721 cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa gttagcggat
      781 gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat gattactttt
      841 gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag ttgagggcgc
      901 attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat tattgacatc
      961 cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt ttaactaaat
     1021 tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa ataaagaagc
     1081 tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac atgttttaag
     1141 agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg tagaaggtaa
     1201 tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag atggattttt
     1261 cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa aatacattga
     1321 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
     1381 tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa
     1441 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
     1501 agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
     1561 attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
     1621 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
     1681 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgacaatga
     1741 atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg aaatactata
     1801 aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa caaataaaaa
     1861 agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga gattttagca
     1921 tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt ggggcaagcc
     1981 gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga atgaagaact
     2041 agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg tttatcttat
     2101 ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag atggcttgat
     2161 tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt acggaccagg
     2221 taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga
     2281 actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg
     2341 attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg
     2401 taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa gactttcttg
     2461 ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct gtctcagtaa
     2521 cggttaaagg acaca
//
LOCUS       DACDSR010000176         2503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDSR010000176 DACDSR010000000
VERSION     DACDSR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.182"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(944..1462)
                     /locus_tag="IWK15_002808"
     CDS             complement(944..1462)
                     /locus_tag="IWK15_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9548328.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          816 a          457 c          360 g          870 t
ORIGIN      
        1 taatcgcaaa tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag
       61 ttatttgttc ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac
      121 gaaagaataa aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag
      181 tgagttagtg cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct
      241 tgcagaaatc ggtactggga ttaaactaag caataaaaac taatgttccc ctcattcttt
      301 gaacttctac tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc
      361 aaatcccgta tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg
      421 ccacataccc tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta
      481 gcatattaac aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa
      541 tttcaattct tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca
      601 tgtgggttgt tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa
      661 acatcccaaa aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat
      721 atggcaccat aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa
      781 ttgatacaat ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc
      841 caataaaaac cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata
      901 caagaggtct cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct
      961 tccattttct tatctttgtt ctaaaagtcc caaatggagc aactgtattt acatgaataa
     1021 atttatatac ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg
     1081 cagtgaatag atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac
     1141 gattgagaat ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact
     1201 tatataactc tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc
     1261 catttctttc atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta
     1321 aattttcagc aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa
     1381 tagtttcaaa ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt
     1441 ctttattttt atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat
     1501 tcatctataa tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat
     1561 tgactgtcta actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa
     1621 tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt tcttttgtag taaaatcgga
     1681 ctggtttgca tatcattttc ttcgtaaata tcaaaatcat gttaactact atcaattact
     1741 aataaaaact accactttaa gcgttaagaa ctgttaagtt tagttaagtg aggatagttc
     1801 acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt atataggatt
     1861 acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag taaaaattga
     1921 cgatgacacg ttccttacgg ctcatcagat gaaacagcta caaaaacgaa atgatcaact
     1981 cgaagaaaat ttaattctaa aaaagcgatt gccatattca cgcctcgctc gacgaactat
     2041 taaaggtcaa gatttacctg aagatcatcc tttttaggaa attgaagctt tttatgttgc
     2101 acaatgcatt tataatacta caatgatgtt ttcaccagat attcttctag ggtgcggtgt
     2161 aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa
     2221 ttatgttaat cttccgaaat ttttttccaa aattaaggga tcaagctggt gttatagatt
     2281 gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc gatactctgt
     2341 taagttggtt gacacggtgt cgatttttat gactttctta ttctgaaact tcactaacgg
     2401 acagtaccta aaaagtctat agtatggacg tttacgagta ctattttatc aaaaatgctg
     2461 ctcatttcaa cttatatgtt ttagtgaact acccccactt agc
//
LOCUS       DACDSR010000177         2408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDSR010000177 DACDSR010000000
VERSION     DACDSR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.183"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IWK15_002809"
     CDS             complement(243..464)
                     /locus_tag="IWK15_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548329.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IWK15_002810"
     CDS             complement(562..807)
                     /locus_tag="IWK15_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548330.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IWK15_002811"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IWK15_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="IWK15_002812"
     CDS             complement(2031..>2408)
                     /locus_tag="IWK15_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9548331.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DACDSR010000178         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDSR010000178 DACDSR010000000
VERSION     DACDSR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.184"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWK15_002813"
     CDS             5..1150
                     /locus_tag="IWK15_002813"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9548332.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWK15_002814"
     CDS             1321..1779
                     /locus_tag="IWK15_002814"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9548333.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IWK15_002815"
     CDS             1938..>2407
                     /locus_tag="IWK15_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9548334.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDSR010000179         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDSR010000179 DACDSR010000000
VERSION     DACDSR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.185"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWK15_002816"
     CDS             207..2069
                     /locus_tag="IWK15_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9548335.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWK15_002817"
     CDS             2069..>2398
                     /locus_tag="IWK15_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9548336.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACDSR010000180         2344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDSR010000180 DACDSR010000000
VERSION     DACDSR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.186"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..515
                     /locus_tag="IWK15_002818"
     CDS             123..515
                     /locus_tag="IWK15_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548337.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="IWK15_002819"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="IWK15_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="IWK15_002820"
     CDS             857..1054
                     /locus_tag="IWK15_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548338.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="IWK15_002821"
     CDS             complement(1097..1672)
                     /locus_tag="IWK15_002821"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9548339.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2344)
                     /locus_tag="IWK15_002822"
                     /pseudo
     CDS             complement(1827..2344)
                     /locus_tag="IWK15_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          750 a          401 c          362 g          831 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaa
//
LOCUS       DACDSR010000181         2233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDSR010000181 DACDSR010000000
VERSION     DACDSR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.187"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWK15_002823"
     CDS             <1..544
                     /locus_tag="IWK15_002823"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9548340.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IWK15_002824"
     CDS             complement(900..1472)
                     /locus_tag="IWK15_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9548341.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IWK15_002825"
     CDS             complement(1488..2093)
                     /locus_tag="IWK15_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9548342.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DACDSR010000182         2104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDSR010000182 DACDSR010000000
VERSION     DACDSR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.188"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWK15_002826"
     CDS             169..1539
                     /locus_tag="IWK15_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9548343.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWK15_002827"
     CDS             1560..1844
                     /locus_tag="IWK15_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548344.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DACDSR010000183         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDSR010000183 DACDSR010000000
VERSION     DACDSR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.190"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IWK15_002828"
     CDS             complement(<1..337)
                     /locus_tag="IWK15_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9548345.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IWK15_002829"
     CDS             complement(404..559)
                     /locus_tag="IWK15_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9548346.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IWK15_002830"
     CDS             1176..1397
                     /locus_tag="IWK15_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548347.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IWK15_002831"
     CDS             1398..1655
                     /locus_tag="IWK15_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548348.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="IWK15_002832"
     CDS             complement(1791..>2058)
                     /locus_tag="IWK15_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9548349.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DACDSR010000184         2009 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDSR010000184 DACDSR010000000
VERSION     DACDSR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.191"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWK15_002833"
     CDS             <1..177
                     /locus_tag="IWK15_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548350.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWK15_002834"
     CDS             190..690
                     /locus_tag="IWK15_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9548351.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2009
                     /locus_tag="IWK15_002835"
     CDS             787..>2009
                     /locus_tag="IWK15_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9548352.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETER"
BASE COUNT          644 a          274 c          455 g          636 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggtt
//
LOCUS       DACDSR010000185         1992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDSR010000185 DACDSR010000000
VERSION     DACDSR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.192"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IWK15_002836"
     CDS             complement(<1..425)
                     /locus_tag="IWK15_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9548353.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IWK15_002837"
     CDS             complement(534..1565)
                     /locus_tag="IWK15_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9548354.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          613 a          328 c          346 g          705 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gc
//
LOCUS       DACDSR010000186         1982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDSR010000186 DACDSR010000000
VERSION     DACDSR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.193"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWK15_002838"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWK15_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9548355.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWK15_002839"
     CDS             852..1040
                     /locus_tag="IWK15_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9548356.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWK15_002840"
     CDS             1053..1610
                     /locus_tag="IWK15_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9548357.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWK15_002841"
     CDS             1642..1884
                     /locus_tag="IWK15_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9548358.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          305 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 tt
//
LOCUS       DACDSR010000187         1963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDSR010000187 DACDSR010000000
VERSION     DACDSR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.194"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..171
                     /locus_tag="IWK15_002842"
     CDS             <1..171
                     /locus_tag="IWK15_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9548359.1"
                     /translation="NNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLY
                     HANEVLKIAEEK"
     gene            172..924
                     /locus_tag="IWK15_002843"
     CDS             172..924
                     /locus_tag="IWK15_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9548360.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            929..1876
                     /locus_tag="IWK15_002844"
     CDS             929..1876
                     /locus_tag="IWK15_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9548361.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          689 a          263 c          390 g          621 t
ORIGIN      
        1 aataataaaa taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat
       61 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
      121 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
      181 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
      241 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
      301 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
      361 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
      421 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
      481 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
      541 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
      601 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata
      661 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
      721 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
      781 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
      841 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
      901 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
      961 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
     1021 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
     1081 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
     1141 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
     1201 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
     1261 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
     1321 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
     1381 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
     1441 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
     1501 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
     1561 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
     1621 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     1681 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     1741 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     1801 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     1861 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     1921 aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DACDSR010000188         1947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDSR010000188 DACDSR010000000
VERSION     DACDSR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.195"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..622
                     /locus_tag="IWK15_002845"
     CDS             <1..622
                     /locus_tag="IWK15_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548362.1"
                     /translation="NYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTAL
                     QLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPR
                     DVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYY
                     MYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            619..>1947
                     /locus_tag="IWK15_002846"
     CDS             619..>1947
                     /locus_tag="IWK15_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9548363.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMII"
BASE COUNT          691 a          232 c          340 g          684 t
ORIGIN      
        1 caattactta aaaaaaagga ctccactata taacaaaagc tataattttt attgtttttt
       61 tcctaatata tatacaggaa gagagaatac aatacaaatt ccattagtta cagctgggtc
      121 aagaaccgca ttgcaacttt cggatatttt taaccctatg atagatattt ataaagaaaa
      181 aaaatatata ttttttacgt ctgtttatga ttttgagggc ggagaacctg tcggagaata
      241 tgaattagta tgcaaaatag cagacttagt aggaaaagat aatctaattg ttaagacgca
      301 tcctagagat gtaagaacaa tttataaaga taatggattt atcgaggata aaaattcatc
      361 aataccttgg gaggcgattc aattgtcagg cgattttaac gataaaatat ttatgacaat
      421 taattcagga agtgttattt caggtagtac aatgtctgca aaacctgtac gcacgtatta
      481 tatgtataaa ttgtgtaaca tagaaggaaa tccgtcttgc aaaaagaatg cgtatgatat
      541 tgagcgattg cttaagcaaa aaacgatgaa aaacatttta aagaatgtga aaatagcaga
      601 aagcttagat gatatactat gagcaaaact aaatatttaa ttaaaaatat gggaatatta
      661 acaataagta atttttcttc aaaaatttta gtttttttgt tagtcccttt atatacaagt
      721 gtattatcca caacagaata tggcacatat gatttgacaa tttcaacagc cacattattg
      781 tttccaattt taaccttaaa cattgtagat gctgtgatgc gctttttaat ggataaagat
      841 aaaaataaaa aaaatatagc atctatagcg attaaagtta tatttttaag cgtgatagtc
      901 tttggatccg taatgtattt gttaaatatt acaaaattat ggcaagatgt ttatggatta
      961 gaaaaatata tttttttgta ttatatatcc tatgtatgga atcaatttct aatacaattt
     1021 gctaaagggt tagaaaaagt taaagctatg ggcatagcag gtattatcag cacaattgta
     1081 acaataattg ccaatatttt cttcttgctt attctgaaat ggggattaat tggttttttc
     1141 attgcaaata ttttatcaca gattatttca gctacttatc tgtcagtatt agtgagaata
     1201 tgggaatata tcgattttga ggctaaagat cctcagctaa agagagaaat gctaaattac
     1261 tcgattccat taattgcaac tgttgttgga tggtggatta atagtacagc tgataggtat
     1321 gtggtttcct ttatagtagg tgtctcggcg aatggcttat tgtctgtggc atataaaata
     1381 cctcaaataa ttaatacttt gcagggaata tttatacagg cctggcaaat ttctgcaatt
     1441 aaagaatatg gagataatga gactgcaaaa ttttatggaa atacatttgt tatgattaat
     1501 ttgttaatgt gcataacctg ttcaagtctt atcatattaa ctagaccgtt ttcacatatt
     1561 ctttatgcga aagactttta tgaagcgtgg aaatatgttc catttttatt aatagctaat
     1621 gttttaaatt gtgcatcagg tctgttagga cctatcttat cagcaaaaaa agattctaaa
     1681 tccatgatgt ggtctgcaat cataggggca ttagttaatg tgattatgag tgtagtcctt
     1741 gtttataaga taggggttca aggtgccaca atagccactg ttatatgttc atatgtaata
     1801 tatgttgttc gtaaacgagc tgtgggaaaa gatattttta taaagcatta tcatattgtt
     1861 gtggaatctt ggggattatt gtttgttcag gcaatcatag aaatatattt taaaaattat
     1921 gtaatagaaa ttataattat gattatt
//
LOCUS       DACDSR010000189         1931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDSR010000189 DACDSR010000000
VERSION     DACDSR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.196"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            73..852
                     /locus_tag="IWK15_002847"
     CDS             73..852
                     /locus_tag="IWK15_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548364.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            898..1842
                     /locus_tag="IWK15_002848"
     CDS             898..1842
                     /locus_tag="IWK15_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9548365.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1858..>1931
                     /locus_tag="IWK15_002849"
     CDS             1858..>1931
                     /locus_tag="IWK15_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9548366.1"
                     /translation="MEETISLKEIFETLRKHLVIIIIS"
BASE COUNT          700 a          334 c          408 g          489 t
ORIGIN      
        1 taaaaagctg tcatttttat gtaagtgaat tatatcttaa tttatctatc atcaatactt
       61 aggagtgaat ctatggaaaa gccattcgtc aagtcattga ctgctgtggc ggtaggtgca
      121 ggtgctattg cgatctgttt gtttggatac catatcaata atcagcggca acatcagcaa
      181 cggatcaatt atgcagaatc tgccatcacc aatcaaaaag acaccttgac ttctttatca
      241 aaagaagtag acaagctgta tagtacaaaa gaaaaaattt ttctaaaccc tgaaataaca
      301 gaagaaacaa tcacaagttt gtcaaataag ctatcctctg tcaaactttc agcagatgat
      361 tacgacatca aagaatcaga attgcctaaa gaagctgttg caatccaaga tgagaagaaa
      421 gcaatcgtga cacagttaga tagtgcagaa tcaaaactga agatccagac agctgtcaac
      481 gatttattta ctaaaaatgt atcaaactgg cagcaagctg tagacgatgt catcatcaaa
      541 gaaaaggtga caagtgcaga tgtcgctcgt gtacgtgaaa atatgagctt ttttaaagat
      601 agtccttgga aaacggttgt tatgcaatat ctaggattcg ctgatattca gatcgcgcaa
      661 gtcacgcaac tagatcagtt attcgatacg atgttaaagg atggtcaagt gactgccacc
      721 gctacttacg atcaatattt gacggtgctc agtcaaatag agcagatccg gaatgaaaaa
      781 ataagcgcag catacgcaac aaaagcacaa actgttgctg agcaaatggg ctatagcaat
      841 ataagctatt gacagttaaa acgttgattc aagcaaggtt agcaaacgga ggaaactatg
      901 gcaagacgaa gaagaaaatc aagagggaaa aaaatatttc tgattatttt agcagttctt
      961 atcgtattag tattagcaat tgtaggagta ggagcaaaat tatatctgga tctctcacat
     1021 tctattgaga aaacttacga atcagtggaa cgaacccaag gagaacgaac gaaaccagtt
     1081 gatctggaca aacaagaatc tttctctgtt ctattgatgg ggattgatac tggagattta
     1141 ggtcgggtcg atcaaggaag atcagatacg atgatggttg ccacagtcag tcctgaagac
     1201 aaacagacaa cgatcgtcag cattcctcgt gatacgtatg tcgacatcgt aggtcgtgac
     1261 acaaaagaca agatcaacca cgcttacgca ttcggcggag ctgccatggc gatggatacc
     1321 gtacaaaaat atgtagacat cccaatcgat cactatgtct ctatcaatat gaaagggcta
     1381 aaggaactcg ttgatgcagt tggtggtatc gaagtcaata atgacctcac tttctcacaa
     1441 gatggctatg actttccaat cggtaaaatt acattgaacg gggatcaggc attatcttat
     1501 tctcgtatgc gttatgaaga cccaaatggt gattacggta gacaagggcg ccaaagaaag
     1561 atcgtagaag gcgtagcgaa aaaagtattg agtctaaacg gtgtcagcaa ctatcaatcc
     1621 gttttgaatg ctctagagtc gaatatgaaa acagacatga actttgatga aatgaaaaaa
     1681 attgctttag attatcggga ggcatttggt acgattaagc aagatcagct gcaaggagaa
     1741 ggcttcatgc aagataatat ctcctatcaa aaagtcagtg atcaagaact tgatcgtgtg
     1801 aaaaaagaat taaaagatca aatgaatcta gagaataaat aggaaagtgg agaaaaaatg
     1861 gaagagacaa tcagcttgaa agaaattttt gaaacattac gcaaacactt ggtaataatc
     1921 atcatctcaa t
//
LOCUS       DACDSR010000190         1921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDSR010000190 DACDSR010000000
VERSION     DACDSR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.197"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IWK15_002850"
                     /pseudo
     CDS             1..492
                     /locus_tag="IWK15_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IWK15_002851"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IWK15_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IWK15_002852"
     CDS             1171..1617
                     /locus_tag="IWK15_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548367.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IWK15_002853"
     CDS             1619..1756
                     /locus_tag="IWK15_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9548368.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
BASE COUNT          689 a          337 c          401 g          494 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 g
//
LOCUS       DACDSR010000191         1911 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDSR010000191 DACDSR010000000
VERSION     DACDSR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.198"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IWK15_002854"
     CDS             <1..969
                     /locus_tag="IWK15_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9548369.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IWK15_002855"
     tRNA            1159..1232
                     /locus_tag="IWK15_002855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          639 a          327 c          362 g          583 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DACDSR010000192         1902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDSR010000192 DACDSR010000000
VERSION     DACDSR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.199"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..1034
                     /locus_tag="IWK15_002856"
     CDS             6..1034
                     /locus_tag="IWK15_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9548370.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1090..1308
                     /locus_tag="IWK15_002857"
     CDS             1090..1308
                     /locus_tag="IWK15_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548371.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1379..>1902
                     /locus_tag="IWK15_002858"
     CDS             1379..>1902
                     /locus_tag="IWK15_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548372.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKE"
BASE COUNT          729 a          212 c          348 g          613 t
ORIGIN      
        1 taataatgaa tagcaaaaaa cttcagatgg gtgaaattgt tagaggtcct agtcaaatat
       61 ctattgatgt tacatataaa tgtaatttta gatgtaaaca ttgttacaat gcgagtggtg
      121 gagacaattg ttttatggat aatgagttga ctgatgatga actgttagaa cttacaaaag
      181 atatatgtaa tttaaagcca tataatattt gtttctgtgg aggagaaact ttactgagaa
      241 aaaaaatagt tttatcttgt gccactttat taaaagaggc aggtattcct aatattgcta
      301 ttgttactaa tggatcatta gtaactcagg atgttgcaaa agaattattc aaagcaggta
      361 ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag tagccatgaa gcacttagat
      421 tatatccaaa cagttttgaa aaggcaatta atgctatcaa attatttaga gaattgtccg
      481 aagaagtgga tgttgctttt tgtcctacta gttttaatta caatcaatta cgagaagtat
      541 atcaactagt aaatactctt aaagtgaagt ctttaagaat tcaaccattt atgcttagtg
      601 gtaggggaat gcaaaatagg gaaatagtgc caactgtttc tcaatataga aaactagttt
      661 ctgatattaa ctatattaaa agaaatgaag attataccaa gatagattgg ggagatccaa
      721 ttgatcatat catccgacac agaactttat tgaaagattt ttcgaatttt gtttctatta
      781 ggtcagacgg aggattaatg gcatcacctt acttgccaat tcaagttgga aatattaaat
      841 gtcacagtct ttctaaatat tggcgagcag gactttataa agttttttct ttagatttag
      901 ttcaagatat agctactcaa gtttcttcaa tacaagatat gagtaaagaa ttaaaaaaag
      961 gacatattaa atggcttaat gaagatatag aaattgatat tctcgatgaa tacaaacatg
     1021 atattttaat ttaggtttat cattggaaag tttagataaa caaagtactt ttagaaagga
     1081 gggaaaaaaa tgatttctga acctcaaatg aataatatgt atgaaagccc aactataatg
     1141 ggagctggtg aagctgcgcc aagagcttgt tcagcaatta tggtgtgtgt gttattagca
     1201 tctgcaggag taggtatcca agcaggagcg gtgtatactt atgctgtagt tgctacagta
     1261 gctgtagctg cagccgcagt ttggaaaaca gtaggattag ttggttagga attaatagga
     1321 gactaactgt ctcctattga ttccaatgat taagctaagg aggaatttta agaaaataat
     1381 gaaatacaat aatttaaaac cgaaattact taatgagaac atagaatata taagagaaga
     1441 aaattatgga tatttaacca tagttcccaa aaatggaact gaattgaaag ataacgtttt
     1501 caatccaaca gtaagatata tattggctaa ctgtactgga aaaaacagtg taaaaaaaat
     1561 agcgaatggt ttaaaaaaga agtttcctag tttagaatat gaaaaattct atcaagatgt
     1621 tgtaggaact attaaatttt tcaaacacta taatattatt gatataggag attatagtat
     1681 ggaagaaaag aaaatatata aaattgaaca aaatttagaa ttagaattac taaatgaatc
     1741 acattatgaa gaaatttata attttatcac agatcatcct gaaagtatgt attttcatga
     1801 agatatttat catcaaggaa atagtaaaga attagaagtt gtccttagat ataatttatt
     1861 ttcgtttacg agtgaatttt actttttaag acataaagag ag
//
LOCUS       DACDSR010000193         1855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDSR010000193 DACDSR010000000
VERSION     DACDSR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.200"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..1469
                     /locus_tag="IWK15_002859"
     CDS             3..1469
                     /locus_tag="IWK15_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9548373.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          563 a          323 c          384 g          585 t
ORIGIN      
        1 gaatgaacaa caaacaatta cgctggttta ctgttgcgtt aattgcattt aacatggttt
       61 ggggcatggg gaatgtagtt aataactatg ctcagcaagg agtatcagtt gtcacctctt
      121 ggctattgat tttggccatt tattttattc catacgcatt gatcgttgga caattaggat
      181 cagccttcaa ggatagtaaa ggaggcgtca gttcatgggt cgaaaatacg acatctaata
      241 aacgactggc atactatgcc gcttggactt actgggtcgt ccatattcct tatttggcac
      301 aaaagcctca agcaattctg attgctggtg gttgggcagt ccaaggaaat ggaaatatcg
      361 taaatacgat gtcagtacaa atggtagcag ttatttcact tattatattt cttgcctttt
      421 tatatttatc aacaaaagga ctttctactt tgaaagtgat tggcggactg gccggaacag
      481 cgatgttcgt catgtcgatc ttatttattc ttttagctgt aacggcaccg ttcatcaatc
      541 caacgatgga atttgccaca ccacatatga atcaagtgaa aacatatatt ccaacatttg
      601 atttcagtta tttcacaacc gtttcgatgc tagtatttgc agttggaggg gcggaaaaaa
      661 tatcccctta tgtcaactct acaagaaatc ctgcaaagga atttcctaaa gggatgatct
      721 tcttagcagc aatggtaggg atttgtgctg tattaggctc agtcgcaatg ggtatgctgt
      781 ttgctagtga taatattcct aaagacttga tggcaaatgg ggcatacagt gcttttcaaa
      841 ttttaggtaa ttactatggt atcggtaact cattgatgat catttacgca ttgacaaaca
      901 tgattggaca aatctccgca ttagcctttt caattgatgc accactaaaa atcttgttag
      961 cagatgcgga tccagaattt gtaccaaact ggctacgtaa acgtaatcgt aaaggtacat
     1021 tggtgaatgg ttatcttctg acaggtatac tagtaggaat cattattttg ttgccgattt
     1081 ttggtatcaa agatatgaat gaattagtaa aatggttgac gaacttgaac tcagttgtca
     1141 tgccaatgcg ttacctatgg gtgttcttgg catatatgat gttgaataaa gcttataaga
     1201 aattcaattc tgaatataaa tttgttaaaa atccaaaaat cggttttatc tttggtgctt
     1261 ggtgctttgc cttcactgcg tttgcatgta tcctaggaat ggtgccaaaa gtagattata
     1321 ctgctgatcc taaagcatgg tggttccaat tatttacgaa tatcatcaca ccaatcgtct
     1381 tgatcttatt aggtatgatc ttgccagcaa ttgctcgtcg agacaaaaac aaaaaaatca
     1441 ctgaaccaga aacagtggtt cagccttaaa atagaaatac ttgttacaag aggttgtgac
     1501 agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg
     1561 tgaagtttcg gcttgttttt cgaagaagct acttctggaa caccgtgaat cagaggttgt
     1621 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagcc cctcatattt
     1681 ttcgtttggc tgggcttatt accgcagatg caagctttgg aacaccgttt aaggaagagg
     1741 ttgtaaaatc agcgatttga cctgagtatt agagaaacaa acggaaaaat agctcctcat
     1801 attttgccgc tttttgagct aatgctgaag gtcagctgat tgaacaccat ttatt
//
LOCUS       DACDSR010000194         1849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDSR010000194 DACDSR010000000
VERSION     DACDSR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.201"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            208..405
                     /locus_tag="IWK15_002860"
     CDS             208..405
                     /locus_tag="IWK15_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548374.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            511..993
                     /locus_tag="IWK15_002861"
     CDS             511..993
                     /locus_tag="IWK15_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548375.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1051..1647)
                     /locus_tag="IWK15_002862"
     CDS             complement(1051..1647)
                     /locus_tag="IWK15_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9548376.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          575 a          276 c          368 g          630 t
ORIGIN      
        1 aataattagt ttttaaggaa tatgttttaa ttttttgaaa aaaattcttc taaatacaac
       61 agctgtttta attgtgaaaa cgaaaacagt acgctataat gcctgtaagg ttgattttta
      121 actgtaaata aaaatcaatg agttaaatct ccaaagaaat agcagtggta atgtggagaa
      181 aagttccagc agtttaggag gattttgatg acggaagaaa tgattaattt aggagaacaa
      241 tatgcatgca aaccaattgg ttttacaaaa acggtcattg gtgaagttgt atcaaaaatg
      301 acaaactgcg ccgtggtcaa agttgcccaa tgtgctgcag aggaccaaga attattagat
      361 gaaaaagcaa gcatggttgt tgcaaaatat gatacatttg aataaagaaa acaattggac
      421 gtgcggcgtg ctgcaagtcc aatttctttg agaaggtaaa tcagtattct tttcctattt
      481 tctggagaag aaaaagcgag gcgagttgtc atggtaacat tctttacggt tctattgttt
      541 ttattcgttg ttgtgatgtt tttatttctt tggctggttc gaaaagaaat tatttatcgg
      601 actgtgcgaa atcgatgggt ctacttggtg atacccttct tagtgtcact agtcatttgg
      661 tatacactca tcagtcatcc tacagcagat gaattggcaa aagggatctt gtcagcaatg
      721 atttttatta gttttttgtt ggacagtaga ggaatcacag aagaaggact tgttttgaat
      781 agctttgaca aaaaaggtgt tcctttttct gagatcaata aaattgtatt atatcagcca
      841 aaaggttcta aaatcgtaaa aatgaatttt ttccgaaacg gatggcgagg accgatgctg
      901 aaattttctg catcattaga agaattagtt ccatttttgt cccaacgctt aaacgatgag
      961 gcagagattg atattatgat tgatccagaa taaaaacaga ggttgcgaca agatgtttgt
     1021 cacaacctct gttttttatt tcaaataaaa ttatccaaat acgcggaagc gatcggcatc
     1081 gttcatgtat tctttttccc cagccggtgt ttctggtgaa ccgaatacta attgcgaacg
     1141 tagtttccag tttgaaggga tattccattc ttttgcaact gcttcgtcaa taactgggtt
     1201 gtaatgttgc aagtttgcgc ccaatccttt atcaactaat gctacccaag tattagctgt
     1261 tgcgattccg tttgattgtt ctgaccaatc tgggaagttg tctgcatata attcaaattg
     1321 ttcttgtaga ccttttacca cgtcagtatc tttgaagaat aatactgtac cataaccatt
     1381 tttgaaacca gctaatttgt tttgtgtatt tgggaaagct tctgctggag tcaatgggcg
     1441 aagtgcttct tcagtgattt cccaaagttt ttcatgtgca tctccgaaaa ggataactgc
     1501 gcgtgttgat tgagcattga aagcagttgg gctttcttta attgcttctt tgatcaaagt
     1561 tgttaattct tcattagaaa gtgaaacatt acgtcctaga tgatagattg aacggcggtt
     1621 tttcaaagtg tcagtaaaag ttgtcataat aaaatttctc ctttgatttg cgttttgcat
     1681 ttttttaatt gattacgaaa tgaagtatag caccttacaa aaagtaagta aacagatatg
     1741 ctcacaaaaa ataagcgaag ttcaaaaaac aattttaata agaaaaatcg ttatgtaagg
     1801 attttcttag ttatcgcttt cattgttgtg ttttaggaat ttttgctta
//
LOCUS       DACDSR010000195         1818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDSR010000195 DACDSR010000000
VERSION     DACDSR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.202"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(165..302)
                     /locus_tag="IWK15_002863"
     CDS             complement(165..302)
                     /locus_tag="IWK15_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9548377.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(304..750)
                     /locus_tag="IWK15_002864"
     CDS             complement(304..750)
                     /locus_tag="IWK15_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548378.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1198..1818)
                     /locus_tag="IWK15_002865"
                     /pseudo
     CDS             complement(1198..1818)
                     /locus_tag="IWK15_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          476 a          383 c          319 g          640 t
ORIGIN      
        1 cttacttttg aaagagtctc agtaattttc gagaaagatg cagcaatcgg tctaattact
       61 aataaaatca aagaaacgat agcggttatt gatcctgcta tcattcccca ttcccctaaa
      121 ttaatcatgt gacaactcct ttaccttaaa taaaaacgca tcaattaaga tgcgctctta
      181 tctttattaa tgattttatc tgcttcttcg tctgtaatgc atagtggaac gaatagtcga
      241 acttgatcgt cagtaaaaca gccccaatca tacatcattt tcacatcgct aaaactaaac
      301 atactactca cctccttttg aagctggatt tagttgctct ttaatttctg aaatgtcttt
      361 gctattttgt aacgaagcaa gcatcatttt tgaattgatt tgtgctaaac tgtcagcttt
      421 ttctttcaat gcagtatttt cctgtttaat cgctacatcg cttagcatga gtttggcatt
      481 tagctgtttt aggttgccgt tttcatgttc aagagcctcg tacatcgctt tgagattgtt
      541 taaatcgttg tgatctaacg cgttcgctaa aataatccat tggttcaatt taggatcaaa
      601 catctgatca gcaatcgtta gcggttcgcc atctgaacga accccttcga gcggtggctg
      661 atctgtgtaa ggaacggata caagcatgtc gtccaatact tttcctgcat actctccgcc
      721 agtacggcca tatttccaaa tgtttttcat ttatttccct cccagtaatt gaatttcggt
      781 ttccaatttg gaatcggtgg ttcgacttct gtgcattctt ccggtaaatg ttcttcgtca
      841 ttcacaatga tttgctcgaa tccgtaaggc ttgattgatt tgtaaactac tttcattaga
      901 tttcctccct taaattgaat aagtgataac aaatgaataa gctgatccat aacttgagtt
      961 tcttcgccat ttaatggctc catctgcacc aatagataac tgagcactgt tcaaagtaga
     1021 acggtctatc gagccaacca gttgctcaaa actaattggt ggccgatagc cttctggaat
     1081 tgttagtatc gttgaatcat ttccaccact gcttttccca ttcaacgcaa aaaaataaat
     1141 agtgacggtt tttccttcac gataaagctt tgctgatccg gtattcccgt ttgtaactgt
     1201 taaggttacg acttcatatt tgttgtcgtc tgatgtcaat acagattttc cttttgattg
     1261 aataccatct tcgaaatttt tcaatccttc gatggattgc ggttcagtca aactaaccgt
     1321 atcattcaag cctttttcag tatattcagg cgtgatgtcc cagctgtagt cgttcggatt
     1381 gttgctatct ttcaatcctt caccaaagta tttaaactga ctaatattcg gggttcgggt
     1441 gtcgcctttt tcaattttga gccagtcaat ttggcatgcg ccggccgttt ctttaggcaa
     1501 ctgaaaaatg cgaaggtctt taggcaaacc cggttcaagt ttcgttggtg tgaatgttag
     1561 agaccatacg tctgtcaatc cctcaactgg ttttagttct ccaaaattaa cgttccaata
     1621 attatatgct acaaaggttt gacttgcggg ttttgttcct ttaagcgtga tagtatacgt
     1681 ttgacctatt ataagctctt cttccgtgtt acctttatat atttcgtacg cgctagattt
     1741 aattgggaac gtcgtacctt tatttgcaat attctcaccc aacgatgttt tacctaccca
     1801 gtaagggtca tctagtaa
//
LOCUS       DACDSR010000196         1813 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDSR010000196 DACDSR010000000
VERSION     DACDSR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.203"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            189..482
                     /locus_tag="IWK15_002866"
     CDS             189..482
                     /locus_tag="IWK15_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548379.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            475..771
                     /locus_tag="IWK15_002867"
     CDS             475..771
                     /locus_tag="IWK15_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548380.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            842..>1813
                     /locus_tag="IWK15_002868"
     CDS             842..>1813
                     /locus_tag="IWK15_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9548381.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRI"
BASE COUNT          692 a          246 c          352 g          523 t
ORIGIN      
        1 atggcaattg gcagatagtc aattattatt tcaagaaaaa taacatttta ttttcttatg
       61 ttacttataa gtagttttac atgctttttg actttaaaaa tacatgtgat gatacatgtt
      121 atatgaatta tcaatgtacc cagtaatatg ctattttcat tatgtaaaaa ataacagggg
      181 gtgtgtagat ggagattgat gaaacttgtt tgagtaactc ttataaaagt atgaatgact
      241 tttttgagga aatggaactg gattcagaat tgctctatca attttatctt gcttttcgag
      301 ggcaacagat ttcttttcca atgaaaatgt acgatcgtga gttagttaga aaaagaatcg
      361 aaaacatgat agaacaggaa aaaactgtag atatccgtca attgacagaa gtttatggtt
      421 tctctgcaag atggattcaa aaagtaatta aacaaaaaga aaggagacga gtacatggct
      481 aagctgattg tattaggtgg tttaatttac ggtggatatt ggttatataa atatatgctt
      541 gcgcatccga atatagataa aggaaaagta attaagacag ttggggtaat catagttgga
      601 ctcatagctc taactaagaa aaataatact aatagcccaa aaataatgag aacttatcgc
      661 tgtagaggat gtggaaggga aatttctact agttttaatc catctgaaag ccctcaagga
      721 agagatcgtt gtcatgctgg ctatcgacat tcttgggaaa ggattaacta aatgaacacc
      781 attgagatta attgactgat aaaataagta cgcgagggag aaattgaaag ggggaaataa
      841 aatgaaaaaa tggttgttat tactaattgg aatggggatt ttattcactg gttgtacatc
      901 tgagaatacg aggactaaag agactgtaga aactgataca acaattgagg ctaaaaaaag
      961 taaaacgcaa tcaactgaac aaactacaac agaaatgaaa caatctgtgg aagcaagtac
     1021 aagtgaaagt tcaatcattg aatcttcttc tgaagaggtt aaaaatactt tgtgggatag
     1081 tgagaaatct actcaactag caatttttgt cccacaatgg ggaaagacat taggtcaaga
     1141 atataaaagc tacaatcctc agaacaatgt aagtctgtat ggtacgcctt taccaagtgc
     1201 agttattgat ggaaattgga aaatggcaat caatgaagca ccagttaccg tacaatggtc
     1261 agaagatgga actggtcatg cagatttcaa tttagtagca gtttattctg atgtagaaac
     1321 tggagaatat ttagggcagc atgtatattt ctttggcttt caaaatggac aacctaaagt
     1381 ttatttaacg caacaaaatc aaggcaatga taaaaactac atgtacttca atgaaactca
     1441 gaaccaacag ttaaaacaag gtttcattga tattgtgaat ggacaaactc ctcaaactcc
     1501 agtagtagaa gaatctactc aacaagcaac aactacaacg acacaatcta caagtagctc
     1561 agcaatttca agttgggaag ctgctaaaga aactgtacta aataataaag ctttatggtt
     1621 gtatgattct cctgataaaa atgcatcaaa tactaattta attagcacaa atgaatatat
     1681 gaaagatcta atgcaagatg aaaatggaag ctactattca attaatattg taacggatga
     1741 taatggaaat acaacaatgg ctggtggaaa acaattccgt gtttatacag atggaagaat
     1801 ttctcaacgt att
//
LOCUS       DACDSR010000197         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDSR010000197 DACDSR010000000
VERSION     DACDSR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.204"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWK15_002869"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWK15_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9548382.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DACDSR010000198         1751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDSR010000198 DACDSR010000000
VERSION     DACDSR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.205"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            51..617
                     /locus_tag="IWK15_002870"
     CDS             51..617
                     /locus_tag="IWK15_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9548383.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            762..849
                     /locus_tag="IWK15_002871"
     tRNA            762..849
                     /locus_tag="IWK15_002871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:794..796,aa:Ser,seq:cga)
     gene            1183..>1751
                     /locus_tag="IWK15_002872"
     CDS             1183..>1751
                     /locus_tag="IWK15_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9548384.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          597 a          272 c          345 g          537 t
ORIGIN      
        1 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
       61 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
      121 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
      181 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
      241 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
      301 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
      361 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
      421 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
      481 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
      541 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
      601 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
      661 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
      721 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
      781 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
      841 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
      901 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
      961 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     1021 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     1081 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     1141 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     1201 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     1261 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     1321 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     1381 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     1441 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     1501 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     1561 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     1621 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     1681 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     1741 ctatcaaatg g
//
LOCUS       DACDSR010000199         1749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDSR010000199 DACDSR010000000
VERSION     DACDSR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.206"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(78..449)
                     /locus_tag="IWK15_002873"
     CDS             complement(78..449)
                     /locus_tag="IWK15_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9548385.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(849..1079)
                     /locus_tag="IWK15_002874"
     CDS             complement(849..1079)
                     /locus_tag="IWK15_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548386.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(1173..1511)
                     /locus_tag="IWK15_002875"
     CDS             complement(1173..1511)
                     /locus_tag="IWK15_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548387.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          579 a          336 c          203 g          631 t
ORIGIN      
        1 cattatttct caaaccaatc ttaaaaatca tcaggagtac ttttttgata tacttttatt
       61 aatgctgggc gtaatggtta aaagatttcc actagttcct ctccaccaag ctgagttgaa
      121 aaatgcttat ctgtaaacaa catagacaaa gaggaaataa acgtttccgt atctttctct
      181 gtcccaagga tattaattaa ttttcctact aggtaagtat cattaaactt aagatctgcc
      241 ttcacatcaa aagttttgat aaaagctttt atcatgcgta caattttgtc tttatcgcca
      301 taattgattt caccactcat atcaatatag tcactaatcc ctgaagaaat aatggcaact
      361 gcttcttctt tcgccttatc agataattca tttaatgact gggattgatt gatgacttct
      421 tgagttttat ctgttacgta atctctcatt atcctatcgg catactccgc aaagtaatct
      481 tgaaaaaatt cttctacagg gtaaccaact tcgttttccc aaccaggaga aatttcatta
      541 tcagtactta ttaaatctat taaaaaccaa cccgttctct gagtgttgat tctataatat
      601 ttttaatttc ctatataaat caaaaatatt atagaatcaa taagctaatt aagaaacaaa
      661 ctttagaaac tctagaagaa cttctgaatt tatatagaag tgtgtagcta cttactaaat
      721 atacaaaagt taaaataaaa tactctggat actctttata ctgcattaaa gttagtaata
      781 ttaacagcaa agatatcgaa aaaacaataa cataatatgt gtcaaaattt ttcataataa
      841 gaactccctt actgttgcat actaacataa tccaaaaact cttctacttc agctaaagac
      901 attccacttt ctttagtcca aaaaacagtt ttttctatcg atccttctct gtaaactgta
      961 aatgtcggag tcgagttaat tgagtagcga ctcttgaatt ctttccactc attttcatct
     1021 tctctaactt ttgctacatt cacgtaataa atattttctt ttcccttttc taccagcata
     1081 tcgaatagct tcctgctttt gatgatcgat ttcttgtgct acatctagta aattttcatc
     1141 aatttctata tcataaagta tttcactagt gtctacatcc acaatatata ctttttatca
     1201 gtagcaattt ttgagtaatc cgttaaaatg atctcagtct cgtcactttc atcagtataa
     1261 atatacgtta acaatgaact caccttttca atcggtgtta gctctaagcc tactgtattt
     1321 tttactattg agtcattatt taccaccata acttcgtact gtatatacca tcctatttgg
     1381 tttccatcaa tatcagtaat tggctcacta gctaatggag tctccggaac aatattttcc
     1441 tgatatttct taaactcttc tgtagcacga aaaaggttat catgttcctc acttgaaact
     1501 ggttgagtca tagaaaatgt tacttggcta aacagaggga aaataaacac tacaatcaac
     1561 aaagtataaa aaaaagccct tctttttcat tttttatctt ccaatcacaa attgaaaaac
     1621 ttattcctgc aactttgagt ctagtatcac aatcgactga ctacaactat tcatttacat
     1681 tctactttaa gttcctcctt aaccattaat tttattaaag atacttgtaa tagtgattct
     1741 cacaaaatt
//
LOCUS       DACDSR010000200         1673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDSR010000200 DACDSR010000000
VERSION     DACDSR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.207"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1372
                     /locus_tag="IWK15_002876"
     CDS             <1..1372
                     /locus_tag="IWK15_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9548388.1"
                     /translation="VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTY
                     PDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVT
                     DVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1506..>1673
                     /locus_tag="IWK15_002877"
     CDS             1506..>1673
                     /locus_tag="IWK15_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9548389.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNL"
BASE COUNT          661 a          248 c          355 g          409 t
ORIGIN      
        1 agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa caacagtaac
       61 agatgttact cccggcggta caattgatac caacacacca ggtaattacg aaggtgtcat
      121 tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag aagtgacaga
      181 caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg
      241 aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag aaggaacaac
      301 agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca attatgaagg
      361 tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt
      421 gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag tgactacaga
      481 cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt taccaaaagg
      541 aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata aaaaaggtac
      601 tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg ttacagtgac
      661 agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga ctacagactt
      721 aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac caaaaggaac
      781 tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa aaggtactgt
      841 agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt ctgtagtaga
      901 taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc aaattgttac
      961 tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa gtcaacacca
     1021 tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag aggcagggac
     1081 aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta ctaatgtagt
     1141 tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta agactgacaa
     1201 tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct caaaagtttt
     1261 tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt taatactgtt
     1321 aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa
     1381 tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt gcttttttat
     1441 gatgggaaat aagtgttagt taaagatgaa tatacttttt atagaaagga aaagaatgta
     1501 attaagtgga aaaaatagta attattggtt caaaccattc agggctagcc gcagctcgat
     1561 ttctaaactt aagcgattca aattatgaag ttattctaat cgataaaaaa tcagaaatta
     1621 gttacttgga atgtgggaca tcgttattta taaaaggaga agtaagtaat tta
//
LOCUS       DACDSR010000201         1667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDSR010000201 DACDSR010000000
VERSION     DACDSR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.208"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..797
                     /locus_tag="IWK15_002878"
     CDS             138..797
                     /locus_tag="IWK15_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9548390.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            816..>1667
                     /locus_tag="IWK15_002879"
     CDS             816..>1667
                     /locus_tag="IWK15_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9548391.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAV"
BASE COUNT          645 a          200 c          315 g          507 t
ORIGIN      
        1 agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat gttattttta
       61 taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt cttaaaaaaa
      121 agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta aataacaaaa
      181 gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt tattatatac
      241 tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt agtaacccag
      301 atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt tccttagatc
      361 aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta gaggctgatg
      421 tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt gaaaaaggac
      481 tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa ttgcaagatt
      541 tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct agaacacagg
      601 atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc aatgtgatga
      661 ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt gagattgagt
      721 cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat aatcatctta
      781 ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag attttagatt
      841 gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat aatacaaaaa
      901 aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg tttctcacaa
      961 ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa ttagcacaga
     1021 taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat ggcgaatata
     1081 atatagagga tcttccagat tatgatggtt catctattga tggaattagg attgcgtttc
     1141 ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa aaaggctact
     1201 tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt ttagcagtaa
     1261 taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt tttggaatga
     1321 tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta gaaaaagata
     1381 tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat gcacaaaaac
     1441 ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac ggaatgggaa
     1501 gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat aatgcaggaa
     1561 aaaaatatca attaaaacca ttattatata taattgatga aatactaaat agtttctatc
     1621 agaagagtta ttggggatat tcattaccaa actatatttc agcagtt
//
LOCUS       DACDSR010000202         1639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDSR010000202 DACDSR010000000
VERSION     DACDSR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.209"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWK15_002880"
     CDS             <1..153
                     /locus_tag="IWK15_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9548392.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWK15_002881"
     CDS             159..575
                     /locus_tag="IWK15_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548393.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWK15_002882"
     CDS             681..944
                     /locus_tag="IWK15_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9548394.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IWK15_002883"
     CDS             944..1216
                     /locus_tag="IWK15_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9548395.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IWK15_002884"
     CDS             1293..1622
                     /locus_tag="IWK15_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9548396.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          625 a          207 c          324 g          483 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaac
//
LOCUS       DACDSR010000203         1637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDSR010000203 DACDSR010000000
VERSION     DACDSR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.210"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            321..1490
                     /locus_tag="IWK15_002885"
     CDS             321..1490
                     /locus_tag="IWK15_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9548397.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          600 a          227 c          259 g          551 t
ORIGIN      
        1 ctatatttta ggtttcatat tatataaatt aataatatac taccatttgt tggtagtata
       61 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
      121 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
      181 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
      241 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
      301 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
      361 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
      421 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
      481 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
      541 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
      601 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
      661 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
      721 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
      781 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
      841 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
      901 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
      961 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
     1021 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     1081 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     1141 aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
     1201 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
     1261 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
     1321 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
     1381 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
     1441 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
     1501 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
     1561 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
     1621 ttcatatgct aatttat
//
LOCUS       DACDSR010000204         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDSR010000204 DACDSR010000000
VERSION     DACDSR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.211"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWK15_002886"
     CDS             101..868
                     /locus_tag="IWK15_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9548398.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWK15_002887"
     CDS             1060..1458
                     /locus_tag="IWK15_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9548399.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DACDSR010000205         1628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDSR010000205 DACDSR010000000
VERSION     DACDSR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.212"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..1525)
                     /locus_tag="IWK15_002888"
                     /pseudo
     CDS             complement(85..1525)
                     /locus_tag="IWK15_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          538 a          267 c          268 g          555 t
ORIGIN      
        1 gaataaatat atgatattca tttgttacac tctcaatctt agctgttgtt ctattcatca
       61 ttactcttat tgtaagtagt tcagtcaaat ttgcttgttc aattgatcga taacagtatg
      121 aattgtttga aaagtaatcg gatatataat tgttttctca ctacctaagc cttcttggta
      181 taaagaacta tcgcaaagta taatccgctg agtacaaaag aaagtgtcgt ctactatttc
      241 tctaagttgt ttacaaatga tattactaat agaaaagtgt tctcccaaaa tgtcttctaa
      301 ctgttgtcga tagtataaat acctaaagtc tgacggagct actatgaaca cttcaattgg
      361 agatttccgt ttatgctcat ttaaaagact taaactaatg gttagtttac gaatagaatt
      421 ctcattccaa cgcagaaata aattgttttg ttttcgccat tgttcaagaa tttcatgaac
      481 ttctaagtac aaagcgcgtt gctcttcatt taacaagtaa gtcttatcag gttgaaatat
      541 ctgaatatca gcccaaagtg agcggataaa cgggagaagt gctttatcaa aaagtatctg
      601 attttcttca gatatattca gagataagat aagtttttga ctgagctcaa tcaccaaagg
      661 gttatctgct acatggtttt gataaacaac gttcaagtct ttttgtaaca attcaaagtt
      721 atcgcataga taatttctag agttgaatag aaagtagata taaaggcatt catcttcgtc
      781 atgacataaa gagaaagaaa gttgctctaa tcctttctgt attagctgaa acaagggagt
      841 atgtcgggca actctttttt cttcctgact aaaatatatt gggttagtct tagatctctg
      901 caaaccgata gcgataccta ataaaataaa ttgtttttca actgtagggt aacttcttga
      961 aaaaaaatgt ttttctacat aatcaatcaa ttgttgaccg ctttgaataa ttgatttatt
     1021 tagctggatc aactctgtcc gatttgtctc atagattatc gttagtaaca aacttcgata
     1081 atccttttca ggaatttcaa aatggccatc agcatttaag tactgcattt gtttaaaaaa
     1141 attaatcaac tcatttcgta agttgtaaac tttgcttaac gaaatgaagg tagtatcagc
     1201 tatattactc caattgtgtt cccctgataa ataatgtcct agtacattga aaaagtcaga
     1261 gcgctgatag atgagttgag tgagtgccaa cctcgaaaac gaagagtcga atgcgactgt
     1321 aaagctactt ctagatttct ccactgaaat aatttgctga tggagtatgt cggtaatgca
     1381 ttccaagtcg ttagtgacag acagtgagtg ggcaaacgtt caattgatct gctatttcgc
     1441 gaatctgaat ggtccctttt tcgtgtaatt gctcacaaat gtaaacttga cgaaaaatac
     1501 ttttctccag ataggtttcc aacatagtac tcctcctagt cattttatac ataactatta
     1561 taactctacc aatcggaata gtcctgtaat atattgaatc aaaaatataa attataaaat
     1621 aattagat
//
LOCUS       DACDSR010000206         1598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDSR010000206 DACDSR010000000
VERSION     DACDSR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.213"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..616)
                     /locus_tag="IWK15_002889"
     CDS             complement(<1..616)
                     /locus_tag="IWK15_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9548400.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLR"
     gene            904..1557
                     /locus_tag="IWK15_002890"
     CDS             904..1557
                     /locus_tag="IWK15_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9548401.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          575 a          224 c          297 g          502 t
ORIGIN      
        1 agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc gtaaatagct
       61 gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac ttcattgatt
      121 tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg taatgcttcg
      181 ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc ttagcgatta
      241 gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt tcctttgtta
      301 gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca caatcatagc
      361 atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact ttcaatatct
      421 gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag tgttcacaat
      481 gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct ttatattctt
      541 tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg agtaattttc
      601 tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc gactaaattt
      661 taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc
      721 ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga tttcaaggtg
      781 tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt caccaacact
      841 agatattata gagccataat atgctgtata atatctagtg tataggctta agtggaggta
      901 tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat aagtgaatat
      961 ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc cttgtcaaaa
     1021 tttaatagcg atataaattt ggttatctta gacattcaga taccttttat aaatggttta
     1081 gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt gactgcattt
     1141 agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata tgtagaaaag
     1201 ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa gaattatgga
     1261 catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta tacagctaaa
     1321 ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa atgtttattg
     1381 gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg gaaagatagc
     1441 gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag aaaaaaatta
     1501 caattagact gtataactac tataagaaat gtaggatata aattggagag aaaatgaaaa
     1561 aattaaagat atttccaaaa atgttcatac aaatattt
//
LOCUS       DACDSR010000207         1558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDSR010000207 DACDSR010000000
VERSION     DACDSR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.215"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..292)
                     /locus_tag="IWK15_002891"
     CDS             complement(<1..292)
                     /locus_tag="IWK15_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548402.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGE"
     gene            complement(294..1352)
                     /locus_tag="IWK15_002892"
     CDS             complement(294..1352)
                     /locus_tag="IWK15_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9548403.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          612 a          256 c          183 g          507 t
ORIGIN      
        1 tttccccctg tataaaaatt tcatcataaa cggataaatc atccgagaaa aattgattat
       61 ggttagttaa caagttaatt ttacgtttaa aggaagaacc gattttccgg tgattcacaa
      121 aaacaacatc attaaatagc cctaattctt ttatacgctc actatatctt ctagcgtttt
      181 caaattcaga ataaataaat aaatctcctt ttgattctcg gtaaaaattt tgataaacat
      241 ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa agctgtcttc atattaattc
      301 tcctttttta aatacaagta agaaatgctt cccaaataat accagaaaac aattgaaatg
      361 acatcgcgct tattgaaaag tacatgagcc tccaccattt catctataaa aatagatagt
      421 ataaaaatta aaacaatacc aagaaacgca taattcactt tacttctaaa aaatgttttg
      481 agtaaactaa agatatttaa cacatagaaa gcatagatac atataaaagc aattatacca
      541 cttgatacaa gtacttgaag aaatccatta tgcatgtttc catgtgctct ttttagttca
      601 ttcacatctg tagtatcaag atctactttt ttcaaaaatt ttgcatcgct attcccatag
      661 aaatcggtct ctccataacc aaataacaaa gttggtctta taactttaag cgcagctttc
      721 caaatcgtaa aacgaccagc tgaaatttcg gagttatcac tagaatgctg taatattact
      781 tctttttctg gaagtggagt tttatctgat tcatttgact ctataacttc tacttttcta
      841 tcaatcgata caggaattgt tgctaaaaaa tccttagcat aattagtacc aaaagaaaaa
      901 tataccaata gaactaataa tatgcttacc atccatttga tttttacagg tagtacttct
      961 ctaatgctct ttctaaattt tgaattggca aacaacaaaa gaaataatac taaaaaagaa
     1021 caaattacta cctgggcacc tctagacgaa gaaagtgtaa tataaaatat ttgtacgata
     1081 aaattcatca tataaaaaac acgaatcgtt tttgttattt ttgaaattaa tacactaatt
     1141 aaacttaaaa taatagaagt atatccaaac atagctccaa catttggact tgtatagaca
     1201 ccaaataacc gatgttcaat aaaaccttgt cttgcaagta atcctggcgt tcctgttggg
     1261 acatgataag aaatcaaaaa aataaattga tagatagata ttagcgcagc taaaaaaatt
     1321 atgataatga aaacattatt aaaatacacc atttttttct ttttttcctc ttcactttgt
     1381 gaaaagtctg caggaaaaat tactatcaaa gtaatcagca aatatccaaa attataaata
     1441 gatggaatta aaaaattttc tctgttaatg attatagtta agataaaact aatcattgct
     1501 ccaatcagta gccaagaaaa acgcatttta aaaaaatttt tctttataaa aacctcat
//
LOCUS       DACDSR010000208         1557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDSR010000208 DACDSR010000000
VERSION     DACDSR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.216"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            77..949
                     /locus_tag="IWK15_002893"
     CDS             77..949
                     /locus_tag="IWK15_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9548404.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1245..>1557
                     /locus_tag="IWK15_002894"
     CDS             1245..>1557
                     /locus_tag="IWK15_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9548405.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CS"
BASE COUNT          486 a          298 c          351 g          422 t
ORIGIN      
        1 aataaaaaaa taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat
       61 aaagggagaa atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg
      121 tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc
      181 tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg
      241 gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta
      301 cattacctcg accccgaaat tggcatggca aaattttgat ttcatcggta ccatgaaaaa
      361 agaatttccg atcccgattt cttggacaac agatgtcaat gcggctgcct atggtgagta
      421 tgtcttcggc agtggcaaag gattgagcag tgtcgtttac tatacgatcg gtacgggtgt
      481 tggcggcggt gccttacaag aagggcgctt tatcgaaggg tttagtcatc cagaaatggg
      541 gcatatgctc gtcgtccctc atccaaaaga tgactttgct ggcagctgtc catttcatgg
      601 caactgttta gaagggatgg ccgctggacc cgccatcgaa aaacgcttag ggaaaaaagg
      661 acaagaggtt gcagaagatg atccttattg ggaaatcgaa gcctcctata ttgctcaatg
      721 tgcctacaat acaaccttga tgttttctcc agatgtgatc atttttggcg gtggtgtgat
      781 gaaacaacgc catatgctgc aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta
      841 tgtcgaaaca cctgagttaa gcaaatatat cgtgacccca gcattagaag acaacgctgg
      901 aacgctcggt tgtttggcat tggctcgaga agcggtctta cattcataaa cgaaaaaagg
      961 ctcttcgaca tttagtgttg gtagagaaag atacaactga aaatttagtc ataaccagtt
     1021 aggcagagtg cttaactggt ttttgtttta cttggaagat caagccttga atgagataaa
     1081 ttcttgctcg aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt
     1141 tttttacacg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
     1201 caaatcgtaa tacccgctaa agcatacaag gagagtgaac ataaatgact tatacccatc
     1261 ttacaccaaa cgagcttgta atgatagaag catattttca tcaagaaact ccggttgcta
     1321 tcgttgcgaa gcagcttaaa cgtggacgcc aaacaattta caatgtctat aactttctca
     1381 aatgtggtgg aacagcactt gaatgctttg aacaatacaa agaaaataag cgacgttgtg
     1441 ggagaaccga aatcattttt cctgctgagg aaaaagaata cattgcaaaa agatcaactg
     1501 aaggttggac cccagacgtc attattggcc gtgcagagcg aaccttctct tgttcag
//
LOCUS       DACDSR010000209         1491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDSR010000209 DACDSR010000000
VERSION     DACDSR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.218"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1447
                     /locus_tag="IWK15_002895"
     CDS             <1..1447
                     /locus_tag="IWK15_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9548406.1"
                     /translation="GFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDTVANGGLG
                     LPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIVLALPFGV
                     STLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGALLAPLIV
                     GTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEERKGFIRNL
                     IIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVTAEEKPRV
                     WAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNPIFIFILT
                     PVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPLWLLGSFF
                     IVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFYTPGTESA
                     YFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          402 a          286 c          315 g          488 t
ORIGIN      
        1 gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga tgtgggaacg
       61 atttagttat tacggtatgc gtgcattact tatctattat atgtacgata cagttgccaa
      121 cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct acggatcttt
      181 gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag gagcaaaacg
      241 cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg ctttaccatt
      301 tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg cctttttgaa
      361 accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc gaagagatgc
      421 aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac cactaatcgt
      481 tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg ctattgggat
      541 gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg gagtgggtac
      601 aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta acttgatcat
      661 cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc atctgacaat
      721 tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt attactttat
      781 caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat gggcttatat
      841 cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt catctatctt
      901 agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga tccaagcaag
      961 ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat tcgtaacgat
     1021 gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc ttggactttt
     1081 atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg gtacaagtgg
     1141 aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg ctgaattgtg
     1201 tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat ttgaatcaca
     1261 aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg cacaaattgc
     1321 acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg cagtagcaat
     1381 cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa tgggctctat
     1441 tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc t
//
LOCUS       DACDSR010000210         1480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDSR010000210 DACDSR010000000
VERSION     DACDSR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.219"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          551 a          169 c          303 g          457 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat
//
LOCUS       DACDSR010000211         1478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDSR010000211 DACDSR010000000
VERSION     DACDSR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.220"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IWK15_002896"
     CDS             287..694
                     /locus_tag="IWK15_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548407.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IWK15_002897"
     CDS             708..1109
                     /locus_tag="IWK15_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548408.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..>1478
                     /locus_tag="IWK15_002898"
     CDS             1111..>1478
                     /locus_tag="IWK15_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548409.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          567 a          182 c          258 g          471 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcac
//
LOCUS       DACDSR010000212         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDSR010000212 DACDSR010000000
VERSION     DACDSR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.221"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..314)
                     /locus_tag="IWK15_002899"
     CDS             complement(<1..314)
                     /locus_tag="IWK15_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9548410.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NY"
     gene            complement(314..1291)
                     /locus_tag="IWK15_002900"
     CDS             complement(314..1291)
                     /locus_tag="IWK15_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9548411.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            complement(1304..>1457)
                     /locus_tag="IWK15_002901"
     CDS             complement(1304..>1457)
                     /locus_tag="IWK15_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9548412.1"
                     /translation="VFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDE
                     FKKIFK"
BASE COUNT          492 a          255 c          156 g          554 t
ORIGIN      
        1 tcataattat attgagtaag atcaatactc tcagaaaaag caggtttttc taaaaatatg
       61 taatcaactt tttcaacaat tacatttagt gtttccagat gcttaaaagt cggatttgta
      121 ataaaagcta tatcataatg tgtatccaaa tcgtctctat tttgaatata cgttatttct
      181 tcaaactctt tttttccact gctactataa gctgttatat caaaattaat agaattcttt
      241 tttccatagc ttattagatt ttttagatgt ctctttccaa ttgaccctaa cccaaaaaaa
      301 attactttca tcattaattt acctcaatgt cgttaattaa gttaataata tacttatctt
      361 tttcaaaagt atattggggt tccattttct tttccaaaag cgtgaaaaat tctttaatct
      421 cctctaaata tgcatcttca ataatagtat gtgaataatt attatcatgc ataacctcat
      481 cgtacaattg aatatctgtc atactagaag tttcttcatt ccatgaaaat agagaatccg
      541 ggttaccttc ccattctatt aaaatctttt caccaattat agttaaattt cttttagcta
      601 ctcttgaaac aacatctaca cttaaagttc caactatacc agtttcatgc tcaataataa
      661 gagtgaaact gtccgaataa tcaatatcaa gattggataa tttctgggtc attacagtaa
      721 acgatttaac tttcccaaat gtatctataa tccaaggtaa ttcaatagcg aatatttctc
      781 tacacccatt agtttttttg tttgaaacaa aaaaattgcg ataatcttcc caaggatgcc
      841 agtcgggtaa atactgtcct acatggtatt gataagaaat ttttttatta tgcgtcactt
      901 tagacttaat atattgaatt tcttttctct tcaaaaatgt agaagataga taaagtaatc
      961 tatctttctt tttagctact tcgattattt catcataata ttcatttaat aaattgattt
     1021 ccgtaaagat attacaatct aaatttaaag cctcttttat aataaattca tgggttatag
     1081 gggatgtaca aacaaaaact gaatttattt caaacaaatt ttttgcctct gttagactat
     1141 tataagttgg tattccaaat tccttttcaa cttcctttcg tctcgcctca ttagtatcaa
     1201 caccacatag taaaatatca ttaaagtgtt cttgaatgag tctaagtctt cttttcccca
     1261 tggaacctaa accaataatt cctatgttca taaaatatca cacctacttg aatatttttt
     1321 taaactcatc aataacaaat tcttgttcac tatctgtcat attacttcca gaaggtaagc
     1381 ataagccgct attaaataac ttttctgaca cactaagatt attatcatgg taatacattt
     1441 ttaccccttg aaatact
//
LOCUS       DACDSR010000213         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDSR010000213 DACDSR010000000
VERSION     DACDSR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.222"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWK15_002902"
     CDS             105..1406
                     /locus_tag="IWK15_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9548413.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACDSR010000214         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDSR010000214 DACDSR010000000
VERSION     DACDSR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.223"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWK15_002903"
     CDS             complement(<1..625)
                     /locus_tag="IWK15_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9548414.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWK15_002904"
     CDS             complement(784..1050)
                     /locus_tag="IWK15_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548415.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWK15_002905"
     CDS             complement(1040..1396)
                     /locus_tag="IWK15_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9548416.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DACDSR010000215         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDSR010000215 DACDSR010000000
VERSION     DACDSR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.224"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..65
                     /locus_tag="IWK15_002906"
     CDS             <1..65
                     /locus_tag="IWK15_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9548417.1"
                     /translation="GVSNRTYKMFSAESEEGGNK"
     gene            62..1081
                     /locus_tag="IWK15_002907"
     CDS             62..1081
                     /locus_tag="IWK15_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9548418.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1270..1343
                     /locus_tag="IWK15_002908"
     tRNA            1270..1343
                     /locus_tag="IWK15_002908"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1304..1306,aa:Ile,seq:cat)
BASE COUNT          464 a          262 c          284 g          383 t
ORIGIN      
        1 tgggtgtatc aaatcgaacc tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca
       61 aatgaaaaag aaaattacta ttactgcgat gagcctgtta acggctcttt ttttattgcc
      121 aattaacgga tttgcctata ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag
      181 ctcacaagta gcaaataatc agtatatttt actgcatgaa acggctaatg aaacagcaac
      241 aggacgcaat gaagcgcagt atatgcaacg ttcatggact agcgcttata ctgcttatat
      301 tgtgggagac ggcggaattg tttaccaagt cggacaacct ggttatgtac agtacggtgc
      361 tggttcatat gctaatgcca atagtcctgt gcagattgag ttacaacaca cacatgataa
      421 agcaactttt gaaaaaaact ataaagcata cgttgaattg gctagagatt cagcaatgaa
      481 atatggtatt ccattaacat tagacacgcc ttataaccaa ccaggaatca aatcacattt
      541 atgggtaact caaaacattt ggggcgatca cactgatccg tatggatact tatcagaaat
      601 gggtgtaagt aaggaaaaac tggcttacga tttagctcat ggatttacag acgaaaaccc
      661 aacgacttct gagaacaagc ctgtcattga tccaacccga gcaggtgcag caaatccaac
      721 tttatcagat ggaacgaacc attctcacat tgatcagttt ggagaaatcg aaaatgcgaa
      781 cttacacgtc gctggatggc acatcgctaa ctataagtac gagtatattt tcattatgga
      841 ctacaatact gggaaagaat tagctcgagt aagagctgat ggaatttata gaccggatgt
      901 aaatcaagct tataatactt ctggaaatgt tggctatcat gtatctttta acatgcgtaa
      961 cttccctaat aagaaagtct atgtcatgat gcgggcaacg aatgatccag agggaaacac
     1021 taaaggcggt gcgcaagatt tccatgacaa acgttggtat ttaaatattc ctaaacgata
     1081 aaaatagctc ctcgttgagg agcattacat aactatattg acaactataa aaatcattcg
     1141 ataaaatagt gatgttatcg catatcttca ctatcaccca taatagtcac actccaagct
     1201 atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg ctttatgtgg
     1261 caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc gtccggtcgt
     1321 aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt gttagaattt
     1381 tttgaagagt att
//
LOCUS       DACDSR010000216         1390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDSR010000216 DACDSR010000000
VERSION     DACDSR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.225"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..414
                     /locus_tag="IWK15_002909"
     CDS             106..414
                     /locus_tag="IWK15_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9548419.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            441..740
                     /locus_tag="IWK15_002910"
     CDS             441..740
                     /locus_tag="IWK15_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9548420.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            914..>1390
                     /locus_tag="IWK15_002911"
     CDS             914..>1390
                     /locus_tag="IWK15_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9548421.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKL"
BASE COUNT          514 a          198 c          291 g          387 t
ORIGIN      
        1 gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc ccaatcttct
       61 ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt tcaaaataaa
      121 ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt acaagcttta
      181 tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta tcttcaaatc
      241 tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccagaa
      301 gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata
      361 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      421 aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      481 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      541 ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg acacatcttt
      601 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
      661 ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt tggagtcaga
      721 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
      781 atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg aaatattttc
      841 aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa aaattagcat
      901 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
      961 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc
     1021 ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac catttacgaa
     1081 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta ctggttttat
     1141 tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa
     1201 tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa tcaacaccat
     1261 tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac aagcgaagac
     1321 catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga taaatgcaga
     1381 aggtaagtta
//
LOCUS       DACDSR010000217         1385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDSR010000217 DACDSR010000000
VERSION     DACDSR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.226"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWK15_002912"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWK15_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWK15_002913"
     CDS             complement(545..739)
                     /locus_tag="IWK15_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548422.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          493 a          224 c          184 g          484 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt caagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taaga
//
LOCUS       DACDSR010000218         1378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDSR010000218 DACDSR010000000
VERSION     DACDSR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.228"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(250..>1378)
                     /gene="cadA"
                     /locus_tag="IWK15_002914"
     CDS             complement(250..>1378)
                     /gene="cadA"
                     /locus_tag="IWK15_002914"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9548423.1"
                     /translation="GLAVLLAVLPPLVVSGAAFSEWIYRALTFLVISCPCALVISVPL
                     SFFGGIGGASKIGVLVKGSNYLELLAQTETVVFDKTGTLTKGDFSIQTIDTKIDPKKF
                     MQYIASAEQFSTHPIAQSVLEGYAGPLLPTTNIQEFAGEGILAEVDGKQVLAGNHKLM
                     ERFEISFPLSQEIGTLLYLAIDQSYSGYLVIADTLKEDAVDALVQLKQAGVKNTVMLT
                     GDSKKIADHIGKQVGVDKIYSELLPEDKVQRLEEILQRNNKKTAFVGDGINDAPVLAR
                     ADVGIAMGGLGSDAAIEAADVVIMNDQPSKIAEAIHLAKKTLKIVKQNIVFAIGIKIL
                     VLALGAFGFASMGDAVFADVGVTVLAVLNAMRSLHVKKEDE"
BASE COUNT          405 a          301 c          208 g          464 t
ORIGIN      
        1 atcatattca atttttaaca aacttacaag acagtgttat tttatgtgtg tattctttta
       61 tttaatacac atcgtttttt catatttttt ccatcaaaaa aataagttat tttcttttat
      121 ttcattgacc ttattgaatt tcaagctatt ttgaattttc tgcaaaatta aaaacattag
      181 ttattttata atcctatcta ttttctttac atttttcatt aagaaaagga tataaaaaaa
      241 agaggacgtt cattcgtcct ctttttttac atgcaagctt cgcatggcat tcaaaacagc
      301 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
      361 acctaaggca agtaccaaaa tttttatccc aatagcaaag acaatgtttt gtttgacgat
      421 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
      481 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
      541 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
      601 tttattgttt ctttgtaaga tttcctctaa ccgctgcact ttatcttcag gaagcaattc
      661 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgagtc
      721 gcccgtcaac ataactgtgt tcttcacacc agcctgtttt aattgaacca atgcgtctac
      781 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgatcgc
      841 tagataaagc aatgtaccga tttcttgact taaaggaaaa gagatttcaa atctttccat
      901 caatttgtga ttacctgcta atacttgttt gccatcaact tcagctaaga tcccttcacc
      961 tgcaaattcc tggatattag ttgttggtaa aagcggacct gcgtagcctt ctaaaacaga
     1021 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca atatattgca tgaatttctt
     1081 tggatcgatt tttgtatcga tcgtctggat cgaaaagtca cctttcgtta atgtgccggt
     1141 tttatcgaat accactgtct cggtttgcgc aagcaactcc aagtaatttg aaccttttac
     1201 taatacaccg attttacttg cgcctccgat accgccaaag aaactaagtg gcacggaaat
     1261 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
     1321 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcct
//
LOCUS       DACDSR010000219         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDSR010000219 DACDSR010000000
VERSION     DACDSR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.229"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWK15_002915"
     CDS             191..445
                     /locus_tag="IWK15_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9548424.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWK15_002916"
     CDS             446..763
                     /locus_tag="IWK15_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9548425.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDSR010000220         1358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDSR010000220 DACDSR010000000
VERSION     DACDSR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.230"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..>1358
                     /locus_tag="IWK15_002917"
     CDS             78..>1358
                     /locus_tag="IWK15_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9548426.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          461 a          255 c          259 g          383 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg
       61 agaggacgat atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg
      181 atgctgtttt ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg
      241 gaaacgggaa agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac
      301 aatacaatca catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact
      361 gtcgaaccca ttggacggct caaagttatt ttgttcaacc cagacattca atcgcgaatc
      421 atgtcagata taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga
      481 aaaattgtca ggtatctttg accaccgtta ttcgtacgtt gaaagagttt aaaagctact
      541 taccaaagca atctaagagg attctcccca gagtattgat ggttgatgaa tttcgttcgc
      601 acgcttccat agaagataaa atgagcttta tttgcgcaga tggcgaaaca ggacaattaa
      661 ttgatgtctt gcctacgcgt aaattgcctc gattaacaag ctatttctta ggctgcgcta
      721 atcctgaaga agtaaaattt ttagtcacag acatgaacgc cgcctacttc cagcttacca
      781 aacgtattct gccaaatgcg aaagtagtga ttgatcggtt tcatattatc aaacacatga
      841 atcaagcctt taatgagttg cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga
      901 aaagtcaggc agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca
      961 tcaaccatta tgaatacaaa acatggaaaa gttttcgagc accaaaatac ccatttctta
     1021 cggaagcgat gatgattgat cgattgcttg gcttttctac gtccctgaag gaggcgtatc
     1081 cctattttca tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct
     1141 tattggcaga acttcccgaa acgttagatg acggctttcg ggaaaagctc caaaaccttc
     1201 tgacctatga agaaggcatc accaacgcga tgatctatcc ttacaccaat ggaaaaatag
     1261 aagcgaagaa tacccatata aagacaatga aacgagtatc ctacggattt aaatcatttg
     1321 agaacatgag aattagaatc tttttgatca atcaatta
//
LOCUS       DACDSR010000221         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDSR010000221 DACDSR010000000
VERSION     DACDSR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.231"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWK15_002918"
     rRNA            <1..>1355
                     /locus_tag="IWK15_002918"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDSR010000222         1354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDSR010000222 DACDSR010000000
VERSION     DACDSR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.232"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            484..741
                     /locus_tag="IWK15_002919"
     CDS             484..741
                     /locus_tag="IWK15_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP9548427.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(712..1020)
                     /locus_tag="IWK15_002920"
     CDS             complement(712..1020)
                     /locus_tag="IWK15_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548428.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1098..>1354
                     /locus_tag="IWK15_002921"
     CDS             1098..>1354
                     /locus_tag="IWK15_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9548429.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSA"
BASE COUNT          501 a          230 c          251 g          372 t
ORIGIN      
        1 agttgttaaa tttgtttacg aatataaaag aaaggagaga agaatatgga aaaaacagtc
       61 tcggcaaaaa tcgaagattt gaaattagac atactgaaac aagcaaaagt ggcgatggaa
      121 cacgcggtag ataaagaaga ctctgccatg gttgcagcca tagcagagat tttagctcac
      181 gtttagtcat cgtttttacg gtcttctgga agctgaccat agataacagc ataatgaata
      241 tctaaataag cttgaacaat ttcttttggc gaaattgatt cgcctttagt agatacttgt
      301 gattcgtgat ttagccatgc aacaactaaa tccgcagcga tttcaaacgg cgttacactt
      361 tgtgtcagtt gccataaaga atttcacaat atgtatggtc gtggtaataa taccaaagag
      421 caatttgagg aatttatgga aattaaaaaa tacaaaaaat catttattaa aggaggctaa
      481 acaatgaaca aggaaatgag ccaaacaatc aaggtacaaa aaatgataga tgatctaaca
      541 catggaattg atagccaagc cgataaaatc atcaaggaat tgcaaggaca aaaagttaag
      601 gatgcaaaaa tgcttctcaa gactatcaac tttgaaatga atccaacaaa aagaaagctc
      661 gccgatgtgt tggaagaaaa gttggcttcc gctatcaacg agcaagaatt actatttgaa
      721 accgatactt ttaacagctg atgttttata gatgacagtt tcatccgaat ctatatcatc
      781 gccgagagaa aagaatgttg cttttgacaa aaagccgata agacctacaa gcggtcttga
      841 tgttgcaaga gtagataccc caatatcacc ttgcaaggta ttcacgtaaa tttgagaaga
      901 atagaacatt ccatcttttt cttcgatatt tgacgcatca tcgtatccaa cgaccaaagt
      961 atcggcaaat attttgattt tttctccatc aagcatagta attacaaatt ctggtttcat
     1021 aaattttcac ctccttaaca attatttcag cctgtcacac tgataaggaa attataccaa
     1081 agaaaggaat gagaaatatg aatacaccac aaatttttaa tttcgagcaa aacgaagttc
     1141 gaactatttt agtaaatgat gaaccatatt ttgtaggaaa agatgtagca gacgttctag
     1201 gatattcgaa tccgcaaaaa gctattcgag atcatgtgga cttggaggat aagacgcaga
     1261 acgattcgtt caccgtcaat ggaacagcag ttgttttgat taacgagtca ggcctttaca
     1321 gtttaatctt aaaatcaaaa cttccctctg ccaa
//
LOCUS       DACDSR010000223         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDSR010000223 DACDSR010000000
VERSION     DACDSR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.234"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..346)
                     /locus_tag="IWK15_002922"
     CDS             complement(<1..346)
                     /locus_tag="IWK15_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548430.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERES"
     gene            complement(363..>1348)
                     /locus_tag="IWK15_002923"
     CDS             complement(363..>1348)
                     /locus_tag="IWK15_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9548431.1"
                     /translation="FKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQN
                     LTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDP
                     EIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLA
                     QTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSP
                     LISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAK
                     IMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          469 a          227 c          149 g          503 t
ORIGIN      
        1 agctttctct ttcaattaac tgatccaatt tttgataatc cataaattca tcatgtatca
       61 aaaagaataa ataacttgat attttagcat ctagcttaat cagattaaat aatgtttgtt
      121 taaaattgct accattaata tgactgatag attctctagc ttcttcacat aaacttttta
      181 ctttattaat tactactttg tcatattcat ttaacacact attattacca ttacttagac
      241 gctcagattc tacgaaacaa ctcaaatcag aaatttccca aaaaaatttt ttgttaaaat
      301 ccaatttcct caactcctcc aaactacgta aatgatatag tctcataata aaataaaacc
      361 tctcagctaa tgttgttgaa attgtccact aaacctaatt gatattgctt aggagataca
      421 cctgcgtact ttttgaatac tctcgaaaag tgtgaagaat ctgaaaatcc taaacctaaa
      481 gaaacttcac tgatagtatg gtttgatttc aacattattt tagccgtaga aatttttcgc
      541 ttagagatat aattataaat cgtaatatgc atttcattct taaagctttc tcttaattta
      601 gtttcactta cagaaaaata attagctatt tcctttacag taattctttt atagatattt
      661 aagccgatga attgaacgac agaagaaatt aagggagaaa gatgctctat cttagttttt
      721 ccgatttcct tagtataata aacaatccca gaatccctca cttttaagac ctcaggtaac
      781 gatacagcta attcgttttc ttgtatcaaa gcatctctac tttgatacgc ttctattata
      841 tccattccta atgttataga cgtttgtgct aacttttcaa aaacaatgat agaataattt
      901 ttttcagacc gaatcgtatc tccactaaca cttggaatta ttccattact cagattaaaa
      961 accatgtttt ctaaatttcg aatatctcca tttttaacat attccagtat tttattttca
     1021 taaagaaaca gatatatttc tggatcgtaa ttttgtgata acagattatc tattttgatt
     1081 tgctcgatag ttttacttag attttctgta tattcaatta acggttcatg gaatagatct
     1141 tctatttttc cagtaaaaaa ataatgaact aaaatcagaa tatctcgaat gtctcctaaa
     1201 gaatacaaag tcaaattttg caatgaatta tagagtcgtt ctctatcagc cattgcaaca
     1261 ttcaaattgt tcatttttag tttaaaaaaa actttatcaa tattatttgc tctaaatgga
     1321 ccaaataagt aatatctggt tttaaaca
//
LOCUS       DACDSR010000224         1346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDSR010000224 DACDSR010000000
VERSION     DACDSR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.235"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWK15_002924"
     CDS             <1..1244
                     /locus_tag="IWK15_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9548432.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          438 a          244 c          286 g          378 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgcttac
     1321 ttttcttctt caagaccaaa taaaaa
//
LOCUS       DACDSR010000225         1342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDSR010000225 DACDSR010000000
VERSION     DACDSR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.236"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1204
                     /locus_tag="IWK15_002925"
     CDS             <1..1204
                     /locus_tag="IWK15_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9548433.1"
                     /translation="LVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETIVRFEQ
                     YNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFI
                     AKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGET
                     GNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFH
                     IVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFR
                     APKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDD
                     GFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFL
                     INQLINVR"
BASE COUNT          428 a          270 c          262 g          382 t
ORIGIN      
        1 gttggtcgtc gatgctgttc tctcgcctac tcctcgtgct tgtagaagtt gtggttctac
       61 tgtggtagat ggaaacggga aagcaattat agtgaaaaat gggaagaagg aaacgattgt
      121 ccgttttgaa caatacaatc atatgccttt ggttacgcgc ctaaaaaagc aacgctatac
      181 ctgtaaaaac tgtcgtaccc attggacggc tcaaagttat tttgtccgac tcagccattc
      241 aatcgcagat catgttcgat ataaaattgc ttctttactg actgaaaaag tatctttgtc
      301 ttttattgcg aaaagttgtc aggtatcttt gactaccgtt actcgtacat tgaaagagtt
      361 taaaagctat ttaccaaagc cttctaagaa gattctcccc agagtattga tggttgatga
      421 atttcgttcg cacgcttcca tagaagataa aatgagcttt atttgcgcag atggtgaaac
      481 aggaaactta attgctgtct tgcctacgcg taaattacct cgattaacaa gctatttctt
      541 aggctgcacc aatccagaag aagtagaatt cttagtcaca gacatgaacg ccgcttactt
      601 ccagcttacc aaacgtgttc tgccaaatgc gaaagtagtg attgatcggt ttcatattgt
      661 caaacacatg aatcaggcac tcaatgagtt gcgtatccgt gaaatgaatg agcttcgtaa
      721 agcaggacag aaaagtcagg cagaaaaact gaaaaagaac tggcgctttc tgctaaaaaa
      781 tcgtgcaagc atcaaccatt atgagtacaa aacatggaaa agtttccgag caccaaaata
      841 cccatttctc actgaagcaa tgatgattga tcgattgctt gagttttctg cgcctctaaa
      901 ggaggcgtat cccttttttc atgaattagt cgaagccttt cgagacaaag accctgactt
      961 atttttctcc ttattggcag aacttcccga aacgttagat gacggctttc gggagaagct
     1021 tcaaaacctt ctgacctatg aagaaggtat caccagcgca atgatctatc cttattccaa
     1081 tggaaaaatt gaagcgaaga atacccacat aaagacaatg aaacgagtat cctacggatt
     1141 taaatcattc gagaacatga gaattagaat ctttttgatc aatcaattaa tcaatgtaag
     1201 ataacaaaaa atctgaacca gaatgtggtt cattctgacc cggatttgat tttgcacaac
     1261 tcatcagtcc ttattgacaa agagccaaaa atctgagcca gaatgaggtt cattctgacc
     1321 cagatttgat tttgtacaac cc
//
LOCUS       DACDSR010000226         1312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDSR010000226 DACDSR010000000
VERSION     DACDSR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.237"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..804)
                     /locus_tag="IWK15_002926"
     CDS             complement(<1..804)
                     /locus_tag="IWK15_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548434.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEP"
BASE COUNT          427 a          212 c          143 g          530 t
ORIGIN      
        1 tggttcctta tctatatttt ctacaaaatc cttattatct tgtgatttag cttcgtataa
       61 ttctttgtat gtcgacacat tggttatact aattcctata gctagttctt gagactctac
      121 ttttctctct agctcctttg acaaagtgat ttgagattta tttggttccg ttggagtcaa
      181 ttctatagat tcttcataaa aatctctata cataaagcta tcattaattt tataaaaaaa
      241 attaaatgtt tttagcactg ccatataaca ttcaaaacat tcttctatag ttggtttact
      301 tccttgagga gaatgaacca atttattacg tttttctatg aaatttttaa tttcctttct
      361 tatttctttc ggaaactcaa tattaaagta ccctttaaac atgctcccta ataatttgtc
      421 tacaggggga gattgtaaat tctcaaatat tagagttaaa tcaggtttct cttgttgata
      481 aaattccttt acccctattt cacaggctgt aattatcgaa ataaacctat ctctagtatt
      541 tgatatattt ttacttcttt taaggagtat aagagaaaag gggatacttt cgtgtccatg
      601 catgtaatta actaattgtt gagcttcaga agttatatct ctaattatat agttaacgga
      661 attaggaagt gtctttaact ctaatgaaat ttctatccaa ttactatctt ctaatctgta
      721 tatgctcgaa gtataatttg attcgtcagc aagcatcgct acatcccaaa cactgatcat
      781 aaaatctttt aaacgttcaa tcatttcagc aatattgcta aaatcagcta atttttcttt
      841 agaataaaaa aaacatttat atcagaatca ataaattcta ttttttttat tactatatcg
      901 tcatcattat cttgaccatt tatgtttatt gttccctgag tggggagtac ttctttcaaa
      961 ttcaagttaa atttatattc cattacttct cctccaatat ataaaaatta cttaactgta
     1021 tccaaattta atatgtaaat aaatcattaa tttttagcta aattctatga gaagtttatt
     1081 tttgttgttt ttgctaatta actaaaacta gtacaaaaaa tttaaaatag attattaaac
     1141 ctattgctta ttctattcag tcatatatat aagcgtcatc ctttagtaac taacatccca
     1201 cactacaata gtattataac atatctattc aacttcaaat tgttgaaatt taagccattt
     1261 atatttactt ttcctcaact ttagaaatga ttttactaac cttatatccg ta
//
LOCUS       DACDSR010000227         1297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDSR010000227 DACDSR010000000
VERSION     DACDSR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.238"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            39..656
                     /locus_tag="IWK15_002927"
     CDS             39..656
                     /locus_tag="IWK15_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548435.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          485 a          171 c          207 g          434 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataa
//
LOCUS       DACDSR010000228         1285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDSR010000228 DACDSR010000000
VERSION     DACDSR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.239"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWK15_002928"
     CDS             <1..848
                     /locus_tag="IWK15_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9548436.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          380 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattt
//
LOCUS       DACDSR010000229         1277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDSR010000229 DACDSR010000000
VERSION     DACDSR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.240"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..>1277)
                     /locus_tag="IWK15_002929"
     CDS             complement(27..>1277)
                     /locus_tag="IWK15_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9548437.1"
                     /translation="LAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLEQLYIS
                     PSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFS
                     SLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQ
                     HTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNL
                     ICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEAT
                     SNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITK
                     QFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVL
                     FGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          416 a          177 c          231 g          453 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaa
//
LOCUS       DACDSR010000230         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDSR010000230 DACDSR010000000
VERSION     DACDSR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.241"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IWK15_002930"
     CDS             24..1103
                     /locus_tag="IWK15_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9548438.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DACDSR010000231         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDSR010000231 DACDSR010000000
VERSION     DACDSR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.242"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK15_002931"
     CDS             <1..395
                     /locus_tag="IWK15_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548439.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IWK15_002932"
     CDS             398..1147
                     /locus_tag="IWK15_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548440.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
BASE COUNT          509 a          210 c          254 g          292 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaat
//
LOCUS       DACDSR010000232         1244 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDSR010000232 DACDSR010000000
VERSION     DACDSR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.243"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            363..>1244
                     /locus_tag="IWK15_002933"
     CDS             363..>1244
                     /locus_tag="IWK15_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9548441.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFT"
BASE COUNT          496 a          189 c          245 g          314 t
ORIGIN      
        1 taaaaatata tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt
       61 ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa tcgataacat ttgttctaag
      121 aattagttac gttaaatacg gaacgcaaga tagacttgaa aaacggtaac aaaagaagta
      181 gtatattaag gtgtgatgtg ctacttgaca tttattctgc tgagcgaact ttctggattg
      241 tgtagcctta tatctattca ggacgtatcg agcagattag taaaggtgtt cttgatgatt
      301 ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat
      361 aaatgaaaaa aagattttta gcacttgcca tcgtactagg cactggctta ttgtcaggct
      421 gtacgaatgc cggtgaaaaa acagccgtta gttataaagg cggtacaatc agcgaacaag
      481 aagtaatgga tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg
      541 tttatcaagt atttgaagat aaatatggag atgacgtatc aaccaaagaa atcgattctc
      601 aatatgatca aacaaagaaa caattgggtg attcatttga ttcacaattg aaatctgcag
      661 gttacacaga acaaacattc aaagacagta tcaaacaaag tctagccttt caagaaggtc
      721 taaagaaaca tatcaaattg accgatgaag atttaaaaac ggcttgggaa tctttccatc
      781 cagaagtgga agctcaaatt atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga
      841 aagctgctga taagggagac gatttctcta aattagccaa agacaagtca actgatacta
      901 cgacaaaaga agatggcgga aaagtgaaat ttgattccac tactacaacg gttccggcag
      961 aagtcaaaga agcagccttt aagttgaaag atggtcaagt ttctgatgtg atcacttcta
     1021 ccaatgcttc aacttatact acggaatatt atgtggttaa aatggtgaaa aaccaaaaca
     1081 aaggcaacga catggataaa tacaaaaaag aattgaaaga aattgcaaca gacacaaaat
     1141 taagtgacag taccttccag aataaagtaa ttggcgaagt attgaaagac gctaatgtaa
     1201 aaatcaaaga caaagacttt gaaaatgttt tgagtacatt cact
//
LOCUS       DACDSR010000233         1221 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDSR010000233 DACDSR010000000
VERSION     DACDSR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.244"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1106)
                     /locus_tag="IWK15_002934"
     CDS             complement(<1..1106)
                     /locus_tag="IWK15_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9548442.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN"
BASE COUNT          483 a          176 c          193 g          369 t
ORIGIN      
        1 ttattcgaat tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt
       61 atttcctggt tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac
      121 tctttgtttg cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt
      181 gctataagtg ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc
      241 aatattagtg caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa
      301 atataataga tcactatact catcgtatag gctataaata aagaagaaaa accatttaac
      361 cgccagacaa attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa
      421 ttggttgaac aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt
      481 aattttttta taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta
      541 tagttgttat agttattatt taaaatatgt gtcagcatat cactaggagc cataccactt
      601 aattcttttc ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt
      661 aagaatcccg cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta
      721 gcgatttttt ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa
      781 accatagcga cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag
      841 taccattttt ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa
      901 aatgttaaag cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag
      961 gcggaaaatg tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt
     1021 ttcatagaaa aataagaaac gaccattgtt aaaaaagtga taaaaaaata gtagatatta
     1081 taacttaaaa caagatttcc catcatttta taaaataaaa aagctggata aagagtaagc
     1141 catggataaa aaatattcat cattgttcca tgatgatcga agttattgaa gtttactgga
     1201 gaactccata cattatctag t
//
LOCUS       DACDSR010000234         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDSR010000234 DACDSR010000000
VERSION     DACDSR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.246"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWK15_002935"
     CDS             <1..1076
                     /locus_tag="IWK15_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9548443.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDSR010000235         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDSR010000235 DACDSR010000000
VERSION     DACDSR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.247"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1192)
                     /gene="aph(2'')-I"
                     /locus_tag="IWK15_002936"
     CDS             complement(<1..>1192)
                     /gene="aph(2'')-I"
                     /locus_tag="IWK15_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9548444.1"
                     /translation="TEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEI
                     VYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSG
                     FRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGS
                     GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE
                     LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ
                     NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDG
                     NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQD
                     IVEEY"
BASE COUNT          394 a          209 c           93 g          496 t
ORIGIN      
        1 aatattcttc aactatatct tgatattctt ttgctttctc aatatctata tttccataca
       61 ttcttaatat atcttctcca aaatttgttc ctatttcttc ttcactatct tcaagtaagt
      121 atataaaatc acaatattca tctataattc cagaatctcc aaaatcaatt attccagtta
      181 atctattatt gccatctaac aatagatgat tacaactaaa atcattatgg cataaacact
      241 ttttaccctc aaaaactgtt gttgcattta gtctttccat aaaactttct atataatctt
      301 tttctatatc agttaaatca ttataaatag tttcacgcaa caatatatac tcttctaata
      361 cattttgttt attatcaata gtacattcac taatatctgt ataatctaaa ccgtgcattt
      421 gtcttaaaaa actggcaata tctcgtttta acaaattttg ttcttcttct gacatagtag
      481 aataaatttc tggtgttaaa aaagttcctt taatttcttt ataacctagt atagataatt
      541 catcactaat atacgaatat tcaatattag gaatttttac attagtttct aaatttgtat
      601 ttaaaaaatt atatattgct ttttcttttg cataaccttt tttcttatta gtactaaatt
      661 ttgttttaaa aatgtattca ttattaacta aatatgccac actatcataa ccactaccga
      721 ttatttcaat actatctact ttgaaattat caaagtaatg ctcaattaaa tatttcattg
      781 ccttaacatt tgtggcatta tcatcatatc tatattccat taaataacaa tcttcttttt
      841 tgccctcgtg taattcatgt tctggcaaat cttcaataat tctaaaacca gatttttggt
      901 atgcccttat tgctcttgga ttatttttat gagggtctaa aataactgca ttagcatttc
      961 tttctttttt caaaaattca aaaatcaatt taatatatct tgtaccaatt cctttactcc
     1021 aataatttgg ctctcctata aattgatcca taccatagac tatctcatca gtttttggat
     1081 aatgataatc agtatataac tcatcataca ttttatatat ttgtccatat ccaataggaa
     1141 cattgttata ttcaataatt actctaaaaa cttcatcttc ccaaggctct gt
//
LOCUS       DACDSR010000236         1190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDSR010000236 DACDSR010000000
VERSION     DACDSR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.248"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..479
                     /locus_tag="IWK15_002937"
     CDS             69..479
                     /locus_tag="IWK15_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9548445.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            493..849
                     /locus_tag="IWK15_002938"
     CDS             493..849
                     /locus_tag="IWK15_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9548446.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          421 a          172 c          225 g          372 t
ORIGIN      
        1 tagctaataa ttggtatttt aaaagaaatt actgtagaat tggattataa ttaaaaatga
       61 gtgagaaaat gataaaagta tatacttcgc ctagctgtac ttcctgtaga aaagcaagag
      121 catggttgca agcgaatcaa cttgaatttg aagagaagaa tatttttgcc gaaccgttaa
      181 cagaaagtga gatcaaaaag atcttggcag cgactgaagg aggagttcaa gatattattt
      241 ccactcggtc gaaaatttac gaaaagctgg gcatagattt taacgaatta actttaaagc
      301 aaatgatcgt cttattcaaa gaatatcctt cattattgag acgacctatt ttgattgatg
      361 aaaaaagaat actaatcgga tttaatgaag atgagatccg gtcatttgtc cctcgtgaaa
      421 ttcgatgtgt tgccagaaat ctgatctatt tacaaaacaa taacatctta aattcatagg
      481 gagggagcct tcgtggaagt gaaagtctat ggatcagcgc actgtgtaac aagtctgaag
      541 gcgaaacaat ggttagaaca ccatcaattg ccttatcaat ttgttgactt agaagaacgc
      601 gatctaagta cagaagaagc agaagaatta tgcagttttg aagaagtgca agcagaacag
      661 ctatttgcga cttggtctga ggcttttcac aacattgcga ttgacctttc ctgtgtagga
      721 aaaaaacaac tgattttttt atgcacacaa cataccaatc tcctcagacg tcctttgatt
      781 attatcgatg aagttctatt tattggatac aatgagcagt tgatggaaca gcttatctta
      841 aataaataga ctttaactgt tttaatcaag tcactgagca tttcatacag ttgtaacagt
      901 cgaacactta gcatcaagcg tgttatgtgt tctttttttg actttatttt tctactatag
      961 agacatgctc aatttatgtg gtcaaaattc aaatctaggt ggggatatag agttaaaaat
     1021 atattgacat gtgtaaattt acatgatata ttcatttctg ttgttttaag tagcaaagca
     1081 ataaaatagt attttaaaaa acatataaaa tagttgttga catgttagaa atgacatgat
     1141 aagataactg agttgttaaa aaagaaccac ttgcgaagct tgattctatc
//
LOCUS       DACDSR010000237         1169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDSR010000237 DACDSR010000000
VERSION     DACDSR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.249"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..373
                     /locus_tag="IWK15_002939"
     CDS             <1..373
                     /locus_tag="IWK15_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9548447.1"
                     /translation="IKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPF
                     DNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRN
                     QRLAQRILDNELGCANASEAV"
     gene            complement(357..950)
                     /locus_tag="IWK15_002940"
                     /pseudo
     CDS             complement(357..950)
                     /locus_tag="IWK15_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          355 a          232 c          205 g          377 t
ORIGIN      
        1 tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa aagagtttga
       61 taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat tgagtcataa
      121 aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga aagtagaatt
      181 tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat ttgactatgt
      241 aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac cggttggtta
      301 ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg ctaacgctag
      361 cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc gaggaggctc
      421 attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat aaaatttgtc
      481 aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc ttcaatgtcc
      541 atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa tctttggatg
      601 acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg aattgttgaa
      661 gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt taagttgagt
      721 ctttccttag caacacgata cctatgaagc acatcaacca agatatactt ctcttctttt
      781 tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga aagctccaat
      841 aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg tattttggta
      901 attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat acgcttgtat
      961 accctacttt cttaaaaagt agggtatact caagaatccg attaattatc ttctcaattt
     1021 ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc tgcaaaatgg
     1081 gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa tttgtatcct
     1141 aaaaaatcca taaaaattct ctcacattt
//
LOCUS       DACDSR010000238         1157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDSR010000238 DACDSR010000000
VERSION     DACDSR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.250"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..244
                     /locus_tag="IWK15_002941"
     CDS             <1..244
                     /locus_tag="IWK15_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548448.1"
                     /translation="NIDLENEISKLESIFSDINSLKYLIVEENSDSFLEFEKYLTDLI
                     DNRNNELMRQNIARFLTKLVSEWKSIISQNMDKLRY"
     gene            515..1138
                     /locus_tag="IWK15_002942"
     CDS             515..1138
                     /locus_tag="IWK15_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9548449.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          440 a          152 c          203 g          362 t
ORIGIN      
        1 aaatatagat ttagaaaatg agattagtaa acttgaatct atcttttccg atataaattc
       61 tcttaagtat ttaatcgtcg aagaaaattc ggatagcttt ttagaatttg aaaaatatct
      121 aacagatctt atagataata gaaataatga attaatgaga caaaacattg caagattcct
      181 tactaaatta gtttctgaat ggaaaagtat tatttctcaa aatatggata aattaagata
      241 ttaaggatgt tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta
      301 cctaagaaat acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat
      361 ctacagttta tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt
      421 aaagcgttga tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac
      481 taatagtaaa tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc
      541 ttccgacgat caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa
      601 taatattgct gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga
      661 aaacagacca gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat
      721 tcctagtctt gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt
      781 tcgtagtaaa ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga
      841 ccctgcatta agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga
      901 aaacgaacgg aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa
      961 tggtcgttat actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt
     1021 gatttacgat gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa
     1081 gaacgcagga gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata
     1141 tgttccagtt agaaaga
//
LOCUS       DACDSR010000239         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDSR010000239 DACDSR010000000
VERSION     DACDSR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.251"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            330..>1156
                     /locus_tag="IWK15_002943"
     CDS             330..>1156
                     /locus_tag="IWK15_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9548450.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAF"
BASE COUNT          430 a          135 c          194 g          397 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttga
//
LOCUS       DACDSR010000240         1145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDSR010000240 DACDSR010000000
VERSION     DACDSR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.252"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1143
                     /locus_tag="IWK15_002944"
                     /pseudo
     CDS             1..1143
                     /locus_tag="IWK15_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          492 a          149 c          192 g          312 t
ORIGIN      
        1 tgttatagaa tatatggaaa caaatggtct ttctatccaa gaaactgcta aaaaatttaa
       61 tatcggttca aatagaactc taagtaaatg gatagagcaa tatgaagaag gcggtgcttc
      121 ttcacttgag agccaaaaaa ggggcagaaa aattagtatg aattccaagc taaacattcc
      181 taaaaaactt aaagatgagt ctcttgaaga agaagttatt cgtttaagag cagagaacgc
      241 atatttaaaa aagttagaaa ccttgattca agaacaagat ttgagcaaga agaaatcaag
      301 gttaaaacaa tccttcagct caaaaaagaa tttaaattga atctattact ttcaattgca
      361 caattagcga agtcaactta ttattactgg gtaaaaaaat tagataagcc agataaatat
      421 agcaagataa agcaagaaat tacagcgatt gtaaaagaat caagaaactc ttatggttat
      481 cgtagagtca ctttagcgtt aaagatgaaa ggatatacaa tcaatcataa aacagttaga
      541 aaattaatgt cccaaatggg acttacctgt caaatccgaa taaagcgata taaatcttat
      601 aaaggaacag taggaaaaat tgccaagaat gtgttaaaac gaaatttttc agtagataca
      661 cccaataaaa aatgggtgac agacgtcact gaattcaaga taaaaggaag aaaaatctat
      721 ctatctccta tacttgattt atttaacgga gaaataatta gttatagtat atcaactagt
      781 ccaacatata aactgattga agaaatgctt caacaagcaa ttaaaaaaaa gggaactgag
      841 ggttcactga ttctacattc agatcaagga tggcagtatc agatgccaca atatcaaaaa
      901 aaattaaaag agaataacat tattcaaagc atgtctagaa aaggaaattg cttagataat
      961 tctgtaatag aaaatttttt tggtgtgctt aaatcagagt ttttttatcg agaaaaattc
     1021 cgatcaattg agatatttca aagtaaatta aatgagtata ttaggtggta taataacaaa
     1081 aggataaaac taaagttaaa tggactatct ccagtagaat atcggaaaca gtccattaaa
     1141 tagtt
//
LOCUS       DACDSR010000241         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDSR010000241 DACDSR010000000
VERSION     DACDSR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.253"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK15_002945"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK15_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9548451.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDSR010000242         1127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDSR010000242 DACDSR010000000
VERSION     DACDSR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.254"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1119
                     /locus_tag="IWK15_002946"
     CDS             <1..1119
                     /locus_tag="IWK15_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9548452.1"
                     /translation="LETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLD
                     IPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYL
                     FDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYS
                     MDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINR
                     SSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLS
                     ESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAI
                     EEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          434 a          138 c          191 g          364 t
ORIGIN      
        1 ctagagacag aagttgacaa aattgatatt caaaataaaa agattagttg tgtaaaaaaa
       61 gatggtacaa gattctctca agaatatgac aaagttatat tagctacagg ttcaaagcaa
      121 attatgctag atataccggg gaataattta gctaatattt tttcgataaa gaattttcca
      181 aatgcaaaag agatttttaa agctttggat gatagtacta ttaaaaaagt aggaattatt
      241 ggagcaggat atattggagt tgaaattgct gaagccataa gaaaaagagg gaaagaagta
      301 tatcttttcg atgttgcaga tagagtgttg tcgacttatt acgaccgtag tttttcagat
      361 aaagttgaag aaatactatc aaaaaatgga atacatcttt gtctatcaga aagtcctaaa
      421 aaatatttag gaagaaaaaa gattgaaaaa atagtcacta caagtggtac ccaatactct
      481 atggatatgg tcgtacaagc tataggattt ctatctaata gtccaattgg ggaaaaggaa
      541 ctcttgagag atgttagtgg agcatatcta actaatgaat accagcaaac aaatataaaa
      601 gatatatatg ctattgggga ttgtgctaca acttttttta cgtccattaa tagaagtagt
      661 gttgattttt ctatttctaa tgctctaaga acagcttata ttgcaactca acatattaac
      721 aatcaagatt ttacaccttc tggttcccaa tttacaaatg gcttttctat atttaattac
      781 aatttttacg cagcaggttt gaatttaaaa acagcacgat tattaagtga gtctatagat
      841 tatatagaaa tagaagattt cattagacct gcttttttga gaaaaaatag tcgtgtaaaa
      901 ttaagaatta gctttgaaaa aaatacaaag agaatagtag gtgtacaact ttgtagtcaa
      961 gaagatttat ctggactatt atcaatgttt tctcttgcta tagaagaaca aattacatta
     1021 gacaaattga aaatgttaga ttatttattt catcctagtt ttagccaacc ctataatttt
     1081 attttaaagg cagtaacagg attaaacatt gaaacataat cgttact
//
LOCUS       DACDSR010000243         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDSR010000243 DACDSR010000000
VERSION     DACDSR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.255"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IWK15_002947"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IWK15_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9548453.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DACDSR010000244         1105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDSR010000244 DACDSR010000000
VERSION     DACDSR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.256"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..816)
                     /locus_tag="IWK15_002948"
     CDS             complement(<1..816)
                     /locus_tag="IWK15_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9548454.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSD"
     gene            complement(887..>1105)
                     /locus_tag="IWK15_002949"
     CDS             complement(887..>1105)
                     /locus_tag="IWK15_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HAP9548455.1"
                     /translation="EKNKIVVCIENKILSSESGGQLNKYQIYIERNYSNYKKIFVFLT
                     PDGNEPTNPSWGIITYKNIVEESVKYFV"
BASE COUNT          347 a          200 c          160 g          398 t
ORIGIN      
        1 atcgctacag atttcttgtc gatcactaac acgaacctta tgagcgatat ttctagatat
       61 atggacacaa cattgttgaa attgagcatt tggataaaca ctatgaatac tatcgtcaat
      121 accacttaaa ccatctgtta cgactaacaa aacctcttct aaaccacggt cttttagatc
      181 ttgaagaatc tctttccaaa cataagcaga ttcagttgga gctatagtaa atcctagaac
      241 ctctttagtt ccatccaacc tgataccaat tactatatag acagcttctt ttgatacggt
      301 ttgtcttttt aagggaatat gagtagcatc cataaaaatg actgagtatt tagcttctaa
      361 agttctttct ttaaatgccg aaacatcttc agacacaatt ttactcatat ttgaaatagt
      421 ttgtggtttg taataatgac catacatttt ttcgattaga tcagagattt cggacatagt
      481 aattcctttt tgaactagct gaataatagt ggtttctaaa gagtcattgg ttcttttata
      541 agcaggtaaa gtttgttgag aaaagtctcc attcctatct ctaggtatta ctaaattcaa
      601 ttcaccgtac tctgttttaa aagagcgcga ataattccca ttacgggaat tacctgaatt
      661 aaatccagct ctatcatact tttcatagtc aagaaatacc gttaattcag cttgtaataa
      721 agcgttaata gccaattcta ggtgacgacg aaacaaatca tccaaatcac ctttattgat
      781 tagtatttcc ataatttctg tagtaaaatc gttcatatgg aagtcctctt ttctgtgaat
      841 gtgttgtggt aactctattc tacagaaggg acttcctttt ttctatttac acaaaatatt
      901 ttacactctc ttcaacgata ttcttgtaag taattatgcc ccaactagga tttgtgggtt
      961 catttccatc tggagttaaa aaaacaaaaa tttttttata gttagaataa ttgcgttcta
     1021 tatatatttg gtatttatta agttgccctc cagactccga agacagaatt ttattttcga
     1081 tgcacaccac tattttattt ttttc
//
LOCUS       DACDSR010000245         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDSR010000245 DACDSR010000000
VERSION     DACDSR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.257"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..866
                     /locus_tag="IWK15_002950"
                     /pseudo
     CDS             1..866
                     /locus_tag="IWK15_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          441 a          116 c          169 g          365 t
ORIGIN      
        1 tagcggtatt tattataagt aaagtgaatc ttatttttct gattttttca ctaataatga
       61 tgggagctat ggtgttacag tataatttat ttcaattacc gcttaagaaa acaatcgaga
      121 atacaaaaag tgttaatcaa agtattacag gagaattgaa tgcacatttt gatcaattag
      181 aaattataag cgcatataat tttgagaata tctctatttt aaaattcgat tctagattta
      241 aagaattatt taatgttttc aaaaaacaaa ataaattact aacactggga agctgtttgg
      301 cacaggttat tttccaatta tggattggag gaatatttct tattgatgtt attttattag
      361 ctaaaggaac aataagtatt actaattttg taatagtatt tatgtacgct aacatggtcc
      421 ctactttatg gactacttta attagtatta tttggaaatg gcaagactat tcagtgtcat
      481 taaatagata ttacgaatat tattcaaata acaacaaagt ggctggcaca aaaaaaataa
      541 ataaaataaa ttctattact ggtcaatcat taaatttttc atataacaaa agaaaggtat
      601 taagcaatat aagtttttca actgaaatga ataaactatt catgatatca ggagaaaatg
      661 gaacagggaa aacaacactg atgaaattat ttactaaaca gttgccggtt agtaataatc
      721 aattattcgt caacgggata gatattaata tgattgataa cgaatcatta agaaggttaa
      781 tttcaatcgc aacacaaata ccatatataa ttaatagctc cgtgaaggag aatattttaa
      841 tggggactaa aaactatgat aaaaaaaaat tcagtaaatt aatagaacta ttgggaataa
      901 gtaaatttat gacgaaatta ggtattactt tggacgacaa tatttatgat gaaaaaatat
      961 tattatctgg aggagaaaaa caaaaaataa gtttagctag ggcgcttatt aaatcagctt
     1021 cagttattat attagatgaa ccatttagaa atttagataa tgaaactcgc gataatttga
     1081 gggattatat a
//
LOCUS       DACDSR010000246         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDSR010000246 DACDSR010000000
VERSION     DACDSR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.258"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(128..1006)
                     /locus_tag="IWK15_002951"
     CDS             complement(128..1006)
                     /locus_tag="IWK15_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548456.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          316 a          210 c          178 g          370 t
ORIGIN      
        1 tgtgaatttc agcttatttc cgaaggagtt gcttctgctt ccgccgttta tacgtgttta
       61 gagcgtgaga caaaagtgtt ctttactttt gtctcacgct cttttaaact atttcgaagt
      121 actaaactta tgaagtaaga atacctaagc cagagccaat gtgctcgcct gcaagcaatt
      181 gctcttttgc ttgtcgctgt aaactcgtac tttctggata atcatgtatt tgggcatcta
      241 ctcgtccaaa gatccaaggg actccactat cgtcctgttc atcaactgat aataatcgat
      301 aatcggctgc aaaaagacag atttttgact cttttttcaa ccatttagta agattgggtg
      361 atagtaacca agtatctgtg tacatcactt ggtaatggta atcaggaaaa taaagttcaa
      421 aaaaacgaat ggcactttcg taactatctc ttatcttatt ctgagataaa tctgcatcgg
      481 aaggaatatg aagagaaagg cactgctttc cttctaatct tagatccgtg taccctttcg
      541 gaaaatatgt catttcgtac tccaattcac caatccgaaa gatcaagcca ctagtgtaat
      601 gccaaatcca aaaaccacgg tcaaagcgat agcttcctgt tacttggaaa tgttcatgaa
      661 taaatctagt aaaagcattc attgttgcca aatagatagt tcttggaatc ttcaatggtt
      721 gataaatttt tttccaactt tgagaagctg catatagata aatagctaat gctgcaaaag
      781 gttcctcttc atagactttg tctattttct ttgcagtcgc ctttgcttta ttaggatcca
      841 ttagttcatg actatattgc tcaaacacct catcaaggat gttttttgct tgtacaatca
      901 tttgctctat aacgactttg ggaagagtta aagtttctgc cagaatttgg atttcattgc
      961 ttattttctt catcaaattt ccccttttca tatctttgaa cctcattgtt taaatgactg
     1021 attatccgga caccatatgg tataaacata cttttattgt acactaacta taaa
//
LOCUS       DACDSR010000247         1072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDSR010000247 DACDSR010000000
VERSION     DACDSR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.259"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            16..>1072
                     /locus_tag="IWK15_002952"
     CDS             16..>1072
                     /locus_tag="IWK15_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9548457.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLF"
BASE COUNT          351 a          207 c          213 g          301 t
ORIGIN      
        1 aggacgatac gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat
       61 ttgatgatta cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat
      121 gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga
      181 aacgggaaag caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa
      241 tacaatcata tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt
      301 cgcacccatt ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat
      361 gttagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
      421 agctgtcagg tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta
      481 ccaaagcaat ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat
      541 gcttccatag aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata
      601 gatgttttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg ttgtaccaat
      661 ccagaagaag tagaattctt ggtgacagac atgaacgccg cctacttcca gctcaccaaa
      721 cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc atattgtcaa acacatgaat
      781 caagcgttca atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa
      841 agccaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
      901 aaccattatg aatacaaaac atggaaaagt ttccgagcac caaaataccc atttcttact
      961 gaagcaatga tgattgatcg attgcttgag ttttctccgc ccctaaagga ggcgtatccc
     1021 ttttttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tt
//
LOCUS       DACDSR010000248         1072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDSR010000248 DACDSR010000000
VERSION     DACDSR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.260"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1023
                     /locus_tag="IWK15_002953"
     CDS             <1..1023
                     /locus_tag="IWK15_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9548458.1"
                     /translation="IDDFRSIITSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIW
                     LYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQM
                     FVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSL
                     KEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNN
                     KICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEI
                     SISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTT
                     QVILLF"
BASE COUNT          392 a          120 c          178 g          382 t
ORIGIN      
        1 attgatgatt tcagatctat tattacttcc atggttatga cgtatatgtt ttcatttgaa
       61 gggataaaag ggtatggtta tgtagtattc cagataatgc ttgattcttt gtttttgctg
      121 atgtttattt ggttatatag aaaattagct ataaatagga ttgttgaaca gtccagttct
      181 aaacttactg ctatattcat gatttattta cttacaattg gtttatttgt ttcttatgtt
      241 gctcatcaat atcaagtatt tgatcatttt gtgtttggtg tcttaatatt tttaatcatt
      301 caaatgtttg ttgttctatt tttatttatt agaataacta ttaggcaacg agaacaatat
      361 gaacagcaat tgaaaagaaa agaattggtt tatctaaaaa aatatactga ttcactggaa
      421 aaagatcaag aaaaatttgc cagatttaga catgactata aaaatttatt gctgagttta
      481 aaagaaatcg ctaatcaaaa ccatgattcg atattggaaa aagaaataaa agaattagag
      541 atctattcta attcttatct aaccaataag tttgaatatc gttatttaaa aaatataaaa
      601 aatgaatatt tgaaaagttt gttgatagcg aagctatacc aagcaaataa caataagatt
      661 tgttgtaaat tcgaatgtcc aagtactatt attgaagttc ctgttccaat atttgactgt
      721 attagggtgc ttggaattat tctcgataat gcaattgaag catctataga gagtcaagat
      781 agagaactgt ctctagctat ttaccgagat gataaacaga tagaaatttc gatatcgaat
      841 agttgtagag agatactact ctctatagat actctaatga aaaaaggaat aacaacaaaa
      901 aagggacatc aagggttagg tttatctagt atagaggaaa tcaataaaaa gaatcccaat
      961 atgtttgtga actatcaaaa cgatccgtat caatttacta ctcaagttat tttgttgttt
     1021 tgagggaagg aggatttatg agttatccaa taatattatg tgaagatcaa at
//
LOCUS       DACDSR010000249         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDSR010000249 DACDSR010000000
VERSION     DACDSR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.261"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(206..1036)
                     /locus_tag="IWK15_002954"
     CDS             complement(206..1036)
                     /locus_tag="IWK15_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9548459.1"
                     /translation="MFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSRFVLRLFH
                     ISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLDSRPEQTI
                     EKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASILLAENLD
                     AMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARNYAVANIF
                     LNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLTVVRQKLE
                     "
BASE COUNT          385 a          224 c          184 g          277 t
ORIGIN      
        1 cattgaccat ctcaggaaag tttattcagt taccatcata aacaaaattt tttgatagaa
       61 taaatataaa atttttttga aagttccttt ccacattttt gtgtgaattt gacttaatgc
      121 tgagaatctg gcttttgaac accttgaatt agaggttgtg acaaaaatct tgtcataacc
      181 tcttttggca catatatttg tccttctatt ctaatttttg acgaacaacc gttaaataat
      241 gttgcaaacc aaccgtcatt acccacgcaa cacctaaacc gatgaaactt cctatgacga
      301 tccctaaaaa acgatattcg atcaaagaaa tcaagtatgc tccacgaata agtgtagata
      361 gcatcaacgc catcggatta agaaaaatat tagcaactgc ataatttcta gcaactagaa
      421 attgtgcgat tccatataga aaagtgatca gaaaaatggt ctgcaactgc gtaaacggca
      481 caaatgacaa gaatgccgac acacataacc cacccatcga tccaatcaga tattggactt
      541 gtcgatgctt catcgcatcc aagttttctg ctagcaaaat cgatgcacat gacaaggtca
      601 accaataagg attatgcaga ttcaagccgt gactgacata aacggaaaga aataaggcag
      661 cgctgtagaa aacagaatca atgatcacta aaggctcttc gtgaaatctt tcttttaaag
      721 ttgctttttc aattgtctgc tcaggccgtg aatccagtaa ttttgtaatc actgccgcaa
      781 tcagggcaaa aacgacaccc ataaaaaaat aaagtgagac gatcggtaat tgagcgattg
      841 gcagctgcat gctcgttccc atggcagaaa gcatcgcaaa aaacaagcca cctggtttag
      901 aaatatggaa taatcgcaac acgaatctgc tggaaaaagc aaccaaagct acggcaaatg
      961 gttcaagcca taaaacataa gtcgataaga tccccaaaac gaaagcaacc aacagtccgc
     1021 aaccgatcag aaacatacgt ttcagcaaat atttcatcgg tatgttttga
//
LOCUS       DACDSR010000250         1059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDSR010000250 DACDSR010000000
VERSION     DACDSR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.262"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..993)
                     /locus_tag="IWK15_002955"
     CDS             complement(37..993)
                     /locus_tag="IWK15_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548460.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DACDSR010000251         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDSR010000251 DACDSR010000000
VERSION     DACDSR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.263"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            121..>1045
                     /locus_tag="IWK15_002956"
     CDS             121..>1045
                     /locus_tag="IWK15_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9548461.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFIT"
BASE COUNT          364 a          152 c          230 g          299 t
ORIGIN      
        1 aaattttccg atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac
       61 cattcattta taattagtta tgaagagatg ttcatatgtt gaggcgagga ggtcattaaa
      121 atgaaaagtt acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa
      181 ggagttcaga agatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta
      241 acgattgacg cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg
      301 aaggaattag aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat
      361 ggaaatgaaa aagagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct
      421 ttattgctat tatttgattt taatgaacca attcgcttga tagggtattt attgatttat
      481 ctgctgatag gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc
      541 tttgatgaga attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat
      601 ccagaagccg tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca
      661 gtcagtcatt caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac
      721 gtgcagacga cggaaggatt gatgaccgtg aaccctgaag acgtgcagat cggtcagttt
      781 gtacttgtga agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca
      841 ttagtcgata cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa
      901 acagtattaa gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct
      961 tatgaaaatt ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa
     1021 gcaccagctg aaaatttcat tacta
//
LOCUS       DACDSR010000252         1042 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDSR010000252 DACDSR010000000
VERSION     DACDSR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.264"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWK15_002957"
     CDS             132..344
                     /locus_tag="IWK15_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548462.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWK15_002958"
     CDS             506..1024
                     /locus_tag="IWK15_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9548463.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
BASE COUNT          392 a          133 c          166 g          351 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa at
//
LOCUS       DACDSR010000253         1039 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDSR010000253 DACDSR010000000
VERSION     DACDSR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.265"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            407..1039
                     /locus_tag="IWK15_002959"
                     /pseudo
     CDS             407..1039
                     /locus_tag="IWK15_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          359 a          183 c          186 g          311 t
ORIGIN      
        1 attattaata aaatattttt tatattacaa aagtagtgtt tcaaagttag tagagaaaat
       61 gtagttatat atttaaattt ttaccagaaa gaaatgaaca ccttcaatta tctaaatctc
      121 gaaaatggtt agatgtaatc atcaggaaaa acaatcttaa acgaattact attcatggtc
      181 ttagacacac acatgctagc ttgcttttag caagctagtg caaatattaa agatgtgcaa
      241 gagcgtttag gccactcttc tattcaaatc attatggatc tatatattca tattacagac
      301 aaaagaaaag aagaaaccgt agcgcaattc gcaaaatata ggctctataa tttatgggac
      361 tgatgaatca aaattgattc ttcagtccca ttgttatttt aggtatatga ttcgtacatt
      421 gaaagagttg aaaaactatc ttccgaagcc gtcaaaaagg accctgccaa aagtattgat
      481 ggtggatgaa tttcgttcgc acgcttcaat tgtggataaa atgagcttta tttgtgcgga
      541 tggcgagact ggaaaactca ttgatatttt gcctacacga aaattgtctc gattaacaca
      601 ctattttagt cgctgtgcaa atcctgaagc ggtggaatat ttagtgacag atatgaacgc
      661 cgcctacttc caactgacaa aacgtgtctt accaaatgcg aaagtcgtca ttgattgttt
      721 tcatatcatc aaacatttga atcaggcgtt caatgatttt cgtgtacaag aaatgaaaag
      781 attacaccag ttagggcaac gacgggaagc cgagaaagtg aaaaagaact ggcgctttct
      841 tctaaaaaac cgtcaaaaaa tcaatcacta tgattacaaa acatggcgca gttttcgtgc
      901 acctaaatac ccgctactca ctgaagcaat gatgattgat cgattgctgg cattttcaac
      961 gcctttaaag gacgcctata cttttttcca tgagctgacc gaagccttta gagacaagga
     1021 atcagactta ttttttacc
//
LOCUS       DACDSR010000254         1023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDSR010000254 DACDSR010000000
VERSION     DACDSR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.266"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            273..389
                     /locus_tag="IWK15_002960"
     CDS             273..389
                     /locus_tag="IWK15_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9548464.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          368 a          165 c          160 g          330 t
ORIGIN      
        1 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
       61 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
      121 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
      181 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
      241 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
      301 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
      361 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
      421 aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg
      481 ctgttgattt tccattttta acatatgata aattggtaga atctttcttt tcaaattgat
      541 accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata
      601 aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta
      661 ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg
      721 aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag
      781 cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat
      841 atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc
      901 tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat
      961 aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat
     1021 aat
//
LOCUS       DACDSR010000255          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDSR010000255 DACDSR010000000
VERSION     DACDSR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.267"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IWK15_002961"
     CDS             complement(26..913)
                     /locus_tag="IWK15_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9548465.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DACDSR010000256          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDSR010000256 DACDSR010000000
VERSION     DACDSR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.268"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWK15_002962"
     rRNA            complement(<1..>994)
                     /locus_tag="IWK15_002962"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDSR010000257          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDSR010000257 DACDSR010000000
VERSION     DACDSR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.269"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IWK15_002963"
     CDS             <1..365
                     /locus_tag="IWK15_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9548466.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..914
                     /locus_tag="IWK15_002964"
     CDS             366..914
                     /locus_tag="IWK15_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548467.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          384 a          137 c          204 g          242 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacagagta
      541 gaggaagaac aaaaaaagtt ggcgcaccaa tcagaaagtt tgataaggtt tattccacca
      601 agcatccttt aaaactgtcg actttgttaa atgcgacttc cagagtttta gtagaaaagt
      661 atgagactaa tatgacaaga gatgaattgc ttagaaaagc tttagatgag tatattaaac
      721 agaatcttac gcaagaagat aagcaagatt tgtttaatga cgtggtgaaa gaactagact
      781 tgttcagaga aaaatatcca attgttccag aattagatga tcgtggagaa ataataagaa
      841 ctaccagtca gataaaacaa gataccgaag actcactgcg caaagggtgg ggaatgaata
      901 ataaagttag ataataatca aagaagaact aagtaactac ctttaaacgg tagttactta
      961 gttcttc
//
LOCUS       DACDSR010000258          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDSR010000258 DACDSR010000000
VERSION     DACDSR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.270"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IWK15_002965"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IWK15_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IWK15_002966"
     CDS             complement(570..>959)
                     /locus_tag="IWK15_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9548468.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACDSR010000259          947 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDSR010000259 DACDSR010000000
VERSION     DACDSR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.271"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            48..>947
                     /locus_tag="IWK15_002967"
     CDS             48..>947
                     /locus_tag="IWK15_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9548469.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGIL"
BASE COUNT          313 a          120 c          134 g          380 t
ORIGIN      
        1 aaaaataaat gatactatat gtaatataaa aaaacatgag gtggtatatg aatttgaaaa
       61 aaattttttc tgtaaaaata tttatagttt atgcatttgt tagtttatta tatttatttc
      121 cagcatttaa aaacatggaa atatttggct tttatgatac ggcatttcac attaatcgtg
      181 cgttaagttt agaatctatt tttagctccc ctattaattt tgaaacgttt agatcatatg
      241 gcatgcaagt aaataatttt tacccatggt taactctata tcctttattt ttattgatca
      301 agtttactaa tttagcaata ggctataacc tttttcttta tattgttacg ctcattactc
      361 tatttatatg tcattatgta atgtatgaaa ttaccaaaaa acatgttact agctcctttt
      421 tcgctattat atatacaaca tcttctttta ggagtgtaga aatatttctt cgaggggcaa
      481 tgggggaatt actcgcaatg tcaatacttc ctcttatttt actcgggttt ataaagcttt
      541 acgactcaaa aaaagaatct tgggttatgt tggcgattag catgacttta ttaatttaca
      601 ctcatgttct ttctgtagct atgactatgg taatgattat aattgcttta attattcaat
      661 tttatcggaa aaagaaaata gaaatcttct taattattaa attaataaaa gctgctatag
      721 ttactttact tctttcacta gcgtttatag gtcctatgat tgagcagaca ttgtatcaag
      781 atctgaatag accttatgtg gatatattac aaaaaagagc aatttatctg ggtaaagagt
      841 ttttgattgg aagtatagat tcagaattac tatcatttgg tattggctta actttgctta
      901 tttgtttaat tatactaaca tttaacttta aaaaattagg aatctta
//
LOCUS       DACDSR010000260          942 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDSR010000260 DACDSR010000000
VERSION     DACDSR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.272"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>942
                     /locus_tag="IWK15_002968"
     CDS             <1..>942
                     /locus_tag="IWK15_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9548470.1"
                     /translation="VAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVM
                     KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLE
                     RYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRV
                     KETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADR
                     LLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQ
                     IQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          290 a          191 c          183 g          278 t
ORIGIN      
        1 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
       61 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      121 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      181 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      241 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      301 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      361 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      421 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      481 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      541 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      601 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
      661 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
      721 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
      781 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
      841 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
      901 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDSR010000261          933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDSR010000261 DACDSR010000000
VERSION     DACDSR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.273"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(261..>933)
                     /locus_tag="IWK15_002969"
     CDS             complement(261..>933)
                     /locus_tag="IWK15_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9548471.1"
                     /translation="FHDKVFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNG
                     IELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLL
                     LAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYG
                     RISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKL
                     KESTV"
BASE COUNT          301 a          166 c          112 g          354 t
ORIGIN      
        1 aatctttcgc atcttccacc atacatttaa ttttaattcc cctttatggt acttaataaa
       61 aacaagaaaa gaactattca tttggccctt ttttggttcc agaccataga aatattcctc
      121 cagtaactaa tattgctgtt atgcacgtaa ctaataacag ctataaaaat tggtacaact
      181 ttaataggat tcagaaaaat ttgcactagt gaaggaatct attttattag aaaaatgcaa
      241 tctccctaaa gaaaaaacca ctaaacagtg ctttctttca acttctcctt aattttttgt
      301 gctttcccta aagtaaagtt tctggccaac tctgaatcaa aatatagtga acgtttttta
      361 aaatctattt ctttaacgtt aaagatattt actaaacacg ctcgactaat tcttgttaac
      421 ataggatatt tcttttcaag ctcactaatt cttccataaa attcgtactg cccatcttta
      481 gtacacaatg ataatctatg tggtaatttt gaagcttcta caaaataaat gtcatttata
      541 tcaaaagtaa atgtttgaga acctatggaa aaaacaaatg cttgattcgt aacctgtttt
      601 gttgcatcta tcctttcttg cgctaataac aataactcaa caatctcgtt tcgatactca
      661 tctagtgttt gatcttttgt tacaaatcct aacgtttcaa ctcttctttt tattgttacg
      721 ggtaacatct catcatgagt agttgtaaaa atgatttttg cttgaacatc atattttctg
      781 attacttctg ctaattctat accgttaact tcatgattta aatcaatatc taagaaatat
      841 attccttgct ttggtcggaa ttgtttgagg tattttttaa cttctaacgg actttgtgtt
      901 ttcaatacta tcttaaatac tttatcgtga aaa
//
LOCUS       DACDSR010000262          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDSR010000262 DACDSR010000000
VERSION     DACDSR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.274"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..>855
                     /locus_tag="IWK15_002970"
     CDS             6..>855
                     /locus_tag="IWK15_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9548472.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGI"
BASE COUNT          288 a          116 c          158 g          293 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatct
//
LOCUS       DACDSR010000263          822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDSR010000263 DACDSR010000000
VERSION     DACDSR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.276"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          300 a          145 c          181 g          196 t
ORIGIN      
        1 tgaaaggcat tctccgactt ccaggttttt gaccattttc tctcaacgtg gaaaaaccgc
       61 cacttatgag agaaaatggt caaaaaccta agcgggaagt ctccgaataa cataccaagc
      121 gccgacagcc aacagataaa gaatacacta ctaaaaatag tagtagcata aaatttaaaa
      181 gggaaaaaat caacccctac cccagtcttg gggtctcaaa actaactccc ccctaacccc
      241 ctctttcagc ctcttttgaa agagggggag aatcaacccc acggccaacg ccccagtgtt
      301 cttggggctg tccagaacag aaacttgcga gagagtgggg gtaaaacaag tgtagaggaa
      361 acttgaagac ttgttcgaac agaaacttgc gagagagccg gagtaaaaca agtgtagagg
      421 aaacttgaag acttgttcga acagaaactt gcgagagagt tggagtaaaa caagtgtaga
      481 ggaaacttga agacttgttc gaacacaaac ttacaagaga ggggagtaaa gcagttgcag
      541 aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa agcattatta aattgaaggt
      601 gatgaaatat gaagtattta ttgcatggct attttattga ttattctact cgcaatagta
      661 gtactttagc gattctatgt ttgcttcatg aattgagagt ggttactaga aaacagctat
      721 acaaactatt gaattttgac agatcaatta gtaaacaagg agtaacgaat gctttagtga
      781 gattatatga aaaacagtta atcgatcgaa aaaaatatgg aa
//
LOCUS       DACDSR010000264          815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDSR010000264 DACDSR010000000
VERSION     DACDSR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.277"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>815
                     /locus_tag="IWK15_002971"
     CDS             <1..>815
                     /locus_tag="IWK15_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548473.1"
                     /translation="EFENFIKLCSTLLYLILAYGYACTAFYLFKAEGIDFIFWSGVIS
                     YMSGSVFYLIVSYGLNGLISYIKSLITGETNTANFAMEVHDLTFAMGVFFLYYVFFEN
                     KNTKKHKIKIIVSLILIFMGLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICAT
                     VISLVFIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKY
                     FFNLYLSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTK"
BASE COUNT          252 a           80 c          148 g          335 t
ORIGIN      
        1 agaatttgaa aattttataa agttatgttc cacgttgctt tatttaattc ttgcatatgg
       61 ttacgcatgt acagcatttt acttatttaa ggcagaagga attgatttta ttttttggtc
      121 aggtgttatt tcatatatga gtggttcagt tttttattta attgtatctt atggtttaaa
      181 tggtttaatt tcttatataa aaagtcttat aacaggtgaa acaaatactg caaattttgc
      241 tatggaggta catgatttaa cttttgcgat gggggttttt ttcctttatt acgtattttt
      301 tgaaaataaa aatacaaaaa aacataagat aaaaattatc gtatctttaa ttttaatttt
      361 catgggattg aagagaattg aactgttagc tttaagtggt gcaattctag tgtattatgt
      421 tttattaaag tggggaaaaa agattcaaat ccgtgcatta gtgtgtacta tttgtgcaac
      481 tgttatttcg ttagtgttta tttacattat agatacagga attttggcat tgcttatgtc
      541 aaatttgggc attacagatg acggtgcgag gctttcattt tatctatatt catctaaatt
      601 ttatgaactt ggtttaggat atgtaggaac aggatggaca tggttttcaa aatacttttt
      661 taatctttat ttatcgcact ttaggttaaa tggtggaaga attgcagcaa gcctacacag
      721 tgatatatta actatttata tagaagttgg atgtatagtt tttattatat ggatgttttg
      781 gttatttttt gtgaaaccga taagactaac taaaa
//
LOCUS       DACDSR010000265          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDSR010000265 DACDSR010000000
VERSION     DACDSR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.278"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWK15_002972"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWK15_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9548474.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDSR010000266          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDSR010000266 DACDSR010000000
VERSION     DACDSR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.279"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..607)
                     /locus_tag="IWK15_002973"
     CDS             complement(<1..607)
                     /locus_tag="IWK15_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mg2+ and Co2+ transporter"
                     /protein_id="HAP9548475.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANGSVAKFENQT"
BASE COUNT          252 a          133 c          112 g          279 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgttagcttt ataagttgtt gacaaagcta
       61 tcatgtattt ttgaagagtc aataaatcac caaacgttgg tttgagtgaa ccggacgttg
      121 atatttctga gtctaaatca tcaatttttt ccttgactcc acgaaggtcg ttaagcatca
      181 tatagaaatc tttttgcatg atctttaaaa gtacttcctc cacgaaatga ttgacatcac
      241 gtaaaaccat attggataag agttcgggaa tgaattttaa tttttgtttt gtaaaaatta
      301 tcaacttatt tttaaatacc aagaaaacaa cagatgtcgt cagggtatct agatgcccac
      361 cttcatgtac ctcgggaaac aaaaaactta cgagcaaact tttttcatcg ttcttatcta
      421 tcatcgtgtc atatgcaact cgatcatcaa acgttagtat attgcctatt ttaaagtcat
      481 atttatgttc agaagattcg atatattctc gttcgtcacc ataaacataa acaacagaag
      541 aatttttgat atttgattct gacgaaacta ataatgtatc ttttgttaag gaataagttt
      601 tcagcattgt aatcctcctg ctttatattt ttctcagaaa taaatgcgta attgttgtgg
      661 aataaatacg ttatcagttt taaaaacatt ttgtcttatg tatgataacc tgccaaaaat
      721 tttatctcac acattatcta gacaatcttg tctccagctt ctaatgtatt atctaa
//
LOCUS       DACDSR010000267          750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.280, whole genome shotgun sequence.
ACCESSION   DACDSR010000267 DACDSR010000000
VERSION     DACDSR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.280"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..186)
                     /locus_tag="IWK15_002974"
     CDS             complement(<1..186)
                     /locus_tag="IWK15_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9548476.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHF"
     gene            complement(204..524)
                     /locus_tag="IWK15_002975"
     CDS             complement(204..524)
                     /locus_tag="IWK15_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548477.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT          241 a          138 c           98 g          273 t
ORIGIN      
        1 aaaatgagag gcgctgaaat gtgcaagttc atgagataaa tgaaaggctt ctgcagtttc
       61 gccatataga ttttcattca aaaaaatgat tctaggcttc ggataataga agccaggctc
      121 tttcatttcc atgtaaacta ctttcaaatt gtattcgctc aacatttctt tcaatttcaa
      181 atacatacaa cccatcactc caactattca ttttcctcta aagctttagc aattgcaatc
      241 gctttacgca ttgtctcctt agatatttct tttccatcaa aagaaaaaac agtatcgttt
      301 tctgataaat ccacatgttt aggggtttct ttttcttcgc gccccagaag atagtctaca
      361 gagacatcga aatagtcagc aatttcttgc aaacgttctg tagaggggtt tgagttctta
      421 agtctatata agacgttttt accatagcct aggtcttctt caactttatt taatgatttt
      481 ccctgttttt tcgctaactc ttttattctc tcaaacgtta tcatatcaat attcctttcg
      541 ttcaagaaaa aatatttaac tttatttgtt aaaattcgtt gactatttta acaaaaggtg
      601 ttaatatcat tcttgtaaac aagtttaaca actaaaaaga caacaaaaaa cactattgat
      661 taataaatgc caaccgccaa gaaagctttt aaaatcaatg tttatatgtc ttatttaact
      721 atgttctgat tttaacactg tatgttaatt
//
LOCUS       DACDSR010000268          750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDSR010000268 DACDSR010000000
VERSION     DACDSR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.281"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(91..>750)
                     /locus_tag="IWK15_002976"
     CDS             complement(91..>750)
                     /locus_tag="IWK15_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9548478.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          175 c          143 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg
//
LOCUS       DACDSR010000269          743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDSR010000269 DACDSR010000000
VERSION     DACDSR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.282"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWK15_002977"
     CDS             complement(<1..276)
                     /locus_tag="IWK15_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548479.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          251 a          125 c          125 g          242 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggc
//
LOCUS       DACDSR010000270          737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDSR010000270 DACDSR010000000
VERSION     DACDSR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.283"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            521..>737
                     /locus_tag="IWK15_002978"
     CDS             521..>737
                     /locus_tag="IWK15_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548480.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHY"
BASE COUNT          291 a           79 c          133 g          234 t
ORIGIN      
        1 aaatgaaaat tttttcgtta gtgtattaat gggtgaaagt ttaatcctaa tactgatttt
       61 gtgtttaatt gttcatgaag tgaataaaca attgtgaatt aaaaaaataa aaacctagta
      121 taatcacttt aataaattgt tggtagaagg tgatcaaaat gggagtaaac gaaatgatga
      181 aagtcagatt gagtttttaa atgatacctt cctcagaact tcaaaacgaa ttcaaagatt
      241 cgttgacaaa tcttgagcaa gatcatttaa aaaaatattt tctcttcatt tgacgaaaaa
      301 tgtttagaaa tcttatataa gtgaagataa agtaattggt gtaattaatt gtagagaata
      361 gcctttgagc aattgaagcg atacaattat ttagaaaatg aacttcctcc agcatactac
      421 atgattcctt acgggacgat gttaaagaag aaagcaagaa attttagaag tattagtaga
      481 taaactaatg ggcttttctc aatttaaggg agtagcccaa atgtttatca aatatggtgc
      541 agatacagta gcaaccatgt ttacagaaga atatcaagta gactattatg aagtaataga
      601 taaatgtgta cattttgttt atgaaaaagt gtggaatttt atgaattttg cgataagtga
      661 agaaatctta cgagtacaag aaaacagaac aggaatattt accagagaca gaaagcttaa
      721 tttgattgcg cattata
//
LOCUS       DACDSR010000271          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDSR010000271 DACDSR010000000
VERSION     DACDSR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.284"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IWK15_002979"
     rRNA            2..>733
                     /locus_tag="IWK15_002979"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDSR010000272          732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDSR010000272 DACDSR010000000
VERSION     DACDSR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.285"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..371
                     /locus_tag="IWK15_002980"
     CDS             168..371
                     /locus_tag="IWK15_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9548481.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            371..721
                     /locus_tag="IWK15_002981"
     CDS             371..721
                     /locus_tag="IWK15_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9548482.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          299 a           76 c          119 g          238 t
ORIGIN      
        1 tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc attgttatat
       61 aataaaatgt tatataataa aattactaat gatatttaat attacaagca atcttaagaa
      121 attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg aaacattgtg
      181 ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata gacgttgacg
      241 cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg gtaaattggg
      301 gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt agttttagtg
      361 gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac atgaattata
      421 caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta ctgatgtttt
      481 atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg taaataaatt
      541 agttaattat atttacatcg tgggttttga taataaaata cgctttattg ataatgatga
      601 aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata gtaaatacaa
      661 agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc ctcaacgata
      721 aaatatagta ta
//
LOCUS       DACDSR010000273          727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDSR010000273 DACDSR010000000
VERSION     DACDSR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.286"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWK15_002982"
     CDS             <1..156
                     /locus_tag="IWK15_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9548483.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>727
                     /locus_tag="IWK15_002983"
     CDS             175..>727
                     /locus_tag="IWK15_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9548484.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIK"
BASE COUNT          327 a           55 c          126 g          219 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaa
//
LOCUS       DACDSR010000274          719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDSR010000274 DACDSR010000000
VERSION     DACDSR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.287"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            257..655
                     /gene="spxA"
                     /locus_tag="IWK15_002984"
     CDS             257..655
                     /gene="spxA"
                     /locus_tag="IWK15_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9548485.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          252 a          104 c          137 g          226 t
ORIGIN      
        1 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
       61 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
      121 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
      181 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
      241 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
      301 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
      361 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
      421 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
      481 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
      541 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
      601 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
      661 taatacgaaa cttggaaaag actggctctt tgtcaaatga tgttggtaaa gagaaatct
//
LOCUS       DACDSR010000275          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.288, whole genome shotgun sequence.
ACCESSION   DACDSR010000275 DACDSR010000000
VERSION     DACDSR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.288"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..540
                     /locus_tag="IWK15_002985"
                     /pseudo
     CDS             259..540
                     /locus_tag="IWK15_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            614..>716
                     /locus_tag="IWK15_002986"
     CDS             614..>716
                     /locus_tag="IWK15_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9548486.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT          257 a           91 c          125 g          243 t
ORIGIN      
        1 aagtttttga tcctttattt tcaagtctga tttggcagct tttcggcagc tcatttattt
       61 atggtcagaa atagatgctg aaattttaat ctattagcaa atagtccaga acgtatgaag
      121 aggagtcgga tatcatttgt cattaacacc ataaatattt ataaaattcg tgttcaccta
      181 caattaaatt taatatataa tattcgttgt aagggcttat aattttttgt taatgaacaa
      241 gataggaaga aacatttaat ggacagtttt cagagattgt cactagtaaa tcaatttgag
      301 ttattggcta aatttgagga ccaaaacaga tcttattatg aaagaaaaat cgaaatattg
      361 agagaaggtt acgagtatca ttatgatgat gaaatttgga gtgatctttc tgagccattt
      421 ccgaaagaag attctcgatt tgtattagat gtattgaaca tgtatcgaga tatcaatttt
      481 agtaaatcaa agctaaactt agatgaaaac caaattacta attcatacta tactcatttc
      541 ggttctgttg caaagttttc caaaaaatct attttagtgt aaaattgaga aaaaagacag
      601 agaggacaga gtaatgaatc attttaaagg caaacaattc aaaaaagacg tcattattgt
      661 cgctgttggt tactacctgc gttacaatct aagctatcgt gaagttcagg aattgt
//
LOCUS       DACDSR010000276          715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDSR010000276 DACDSR010000000
VERSION     DACDSR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.289"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..355
                     /locus_tag="IWK15_002987"
     CDS             86..355
                     /locus_tag="IWK15_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9548487.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="IWK15_002988"
     CDS             348..605
                     /locus_tag="IWK15_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9548488.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          276 a           85 c          134 g          220 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatga
//
LOCUS       DACDSR010000277          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDSR010000277 DACDSR010000000
VERSION     DACDSR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.290"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..448)
                     /locus_tag="IWK15_002989"
     CDS             complement(<1..448)
                     /locus_tag="IWK15_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9548489.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DACDSR010000278          703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDSR010000278 DACDSR010000000
VERSION     DACDSR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.291"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..>703
                     /locus_tag="IWK15_002990"
     CDS             132..>703
                     /locus_tag="IWK15_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9548490.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCT"
BASE COUNT          247 a          101 c          114 g          241 t
ORIGIN      
        1 attctaagaa cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc gaaaacctat
       61 ctcattttct gctaatgtaa gttggtttag cttttgttaa tatgacttta tgaaaatgga
      121 ggtaattaaa tatgattttt cctaaagtta atgaagcaag cttgaataaa ggatcctttt
      181 ttcatatcta tgaaaacgaa gattttgaac tgtataatat cctttcacaa aaagaggtca
      241 tcgaaaacca aataatctgc catccaatta tttacaaatc tattttcaag tatgcatttt
      301 ttgcttccaa tgattttcaa agaatggaga ttaccgactg tatattcgaa aactgtgatt
      361 tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc aattgtaaat
      421 taacaggtac taaattgatg gaaagttata ttgggaacac gttattcgag aattgtaaaa
      481 tggactatgt aaatttatct ggttcaaaca taaaagaaag tacctttgag cactctgtct
      541 tatcaagcgc agattttgta gactgttccc tcactaaaat aatgttttct actaatgatt
      601 tagaaaatgc tagctttttt gatactgatt taaaaaatat cgatttaagt agcaactcct
      661 ttgaaatgat tgaagtacgc tcggaacatc taaaaaattg tac
//
LOCUS       DACDSR010000279          684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDSR010000279 DACDSR010000000
VERSION     DACDSR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.292"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>684
                     /locus_tag="IWK15_002991"
     CDS             <1..>684
                     /locus_tag="IWK15_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9548491.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQD"
BASE COUNT          231 a          111 c          135 g          207 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gata
//
LOCUS       DACDSR010000280          649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDSR010000280 DACDSR010000000
VERSION     DACDSR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.293"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>649
                     /locus_tag="IWK15_002992"
     CDS             <1..>649
                     /locus_tag="IWK15_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9548492.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DACDSR010000281          574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDSR010000281 DACDSR010000000
VERSION     DACDSR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.294"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..115)
                     /locus_tag="IWK15_002993"
     CDS             complement(<1..115)
                     /locus_tag="IWK15_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9548493.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..>574
                     /locus_tag="IWK15_002994"
     CDS             246..>574
                     /locus_tag="IWK15_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548494.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNH"
BASE COUNT          207 a           79 c           96 g          192 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acag
//
LOCUS       DACDSR010000282          570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.295, whole genome shotgun sequence.
ACCESSION   DACDSR010000282 DACDSR010000000
VERSION     DACDSR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.295"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..471
                     /locus_tag="IWK15_002995"
     CDS             <1..471
                     /locus_tag="IWK15_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9548495.1"
                     /translation="VLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELKDKKKES
                     LAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFVPTIVAY
                     SKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYGED"
BASE COUNT          226 a           52 c           97 g          195 t
ORIGIN      
        1 gttttagcga tttttatatg ttttgggcta cttttggtag gctacttgtt gtttaattct
       61 aatgaaatga gaactatttc tacgaatgac ttgttaaaaa aaagtgaatt aaaggataaa
      121 aaaaaagaat ctcttgcctt tataatgttt actcaagaag agtgtgagta ttgtatagca
      181 ataaatccaa tcatagatag tgtaaataag gaaactttga ataagataaa gaaatatgat
      241 atatctgtag attttcaatc taaacaaaag tgtaaaatta agtttgttcc gactattgtt
      301 gcatatagta agaataaaga agtggggaga attgacaatg aattagataa tttgtatcga
      361 aaaaagcaag atggttcaat tgtaatagac aagaaaaaaa caaagacatt gcttatagat
      421 tggatgaaaa aaatggataa acaaggagaa tatttgtatg gagaagatta aacagacatt
      481 tccgttaatt aataaatatt ggaataagaa aaaaattttt atatttttta ttacgtttct
      541 gctgtcatct ttattttctc taattttccc
//
LOCUS       DACDSR010000283          561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDSR010000283 DACDSR010000000
VERSION     DACDSR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.296"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>561
                     /locus_tag="IWK15_002996"
     CDS             <1..>561
                     /locus_tag="IWK15_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9548496.1"
                     /translation="SSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVL
                     FVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILK
                     DINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMD
                     YQFFTESVRSELALGNEIVSKDQQEK"
BASE COUNT          221 a           67 c          102 g          171 t
ORIGIN      
        1 gtcttcaaat ttggacttca aagcgactaa gaaacttgct gacttacttt gtgatttaaa
       61 aaataaagga tttactattg ttatagctga acatcgtttt tactatttga aagaattaat
      121 tgatcaagtt ctatttgtta aaaatagaac tgtaaaaact acaactattc agttgttaaa
      181 aactgaaaac aataacgcat taaggaaatt tgacattttt aatagttcga ttgaagttcc
      241 ctatatcgaa aaggtaagtg cagatgtatt aaaagttgaa gaaattactt atgaaaatat
      301 tctaaaagac ataaattttg atgtcaaagt tggagatgtt attggtttgg tagggaaaaa
      361 cggtgtgggg aaaacaacct tactaagaat actcatgaag attattcgcc ctaccaaagg
      421 aaagataatc gaaaataaaa aaaatttgtc atcgccgttt ttagtaatgc aagaaatgga
      481 ttaccaattt ttcacagaat cagtgaggag cgagttagcc ctggggaatg aaatagttag
      541 taaagatcag caagaaaaaa t
//
LOCUS       DACDSR010000284          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.297, whole genome shotgun sequence.
ACCESSION   DACDSR010000284 DACDSR010000000
VERSION     DACDSR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.297"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(330..>533)
                     /locus_tag="IWK15_002997"
     CDS             complement(330..>533)
                     /locus_tag="IWK15_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548497.1"
                     /translation="IVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGY
                     IWEEPGQMRSVLGTYEVEGVPFF"
BASE COUNT          176 a           95 c           60 g          202 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tat
//
LOCUS       DACDSR010000285          529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDSR010000285 DACDSR010000000
VERSION     DACDSR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.298"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          161 a          110 c           74 g          184 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataa
//
LOCUS       DACDSR010000286          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDSR010000286 DACDSR010000000
VERSION     DACDSR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.299"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWK15_002998"
     tRNA            complement(5..90)
                     /locus_tag="IWK15_002998"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWK15_002999"
     tRNA            complement(107..178)
                     /locus_tag="IWK15_002999"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWK15_003000"
     tRNA            complement(190..262)
                     /locus_tag="IWK15_003000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWK15_003001"
     tRNA            complement(280..361)
                     /locus_tag="IWK15_003001"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWK15_003002"
     tRNA            complement(369..441)
                     /locus_tag="IWK15_003002"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWK15_003003"
     tRNA            complement(445..517)
                     /locus_tag="IWK15_003003"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDSR010000287          527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDSR010000287 DACDSR010000000
VERSION     DACDSR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.300"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..329
                     /locus_tag="IWK15_003004"
     CDS             90..329
                     /locus_tag="IWK15_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548498.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          175 a           93 c           83 g          176 t
ORIGIN      
        1 actataaaag acttgatagt tttgcattga ttttgcctaa tactgagcat aggtgtatgc
       61 taaacgatac tataaggagt gtgtacataa tgaaaagaat tctgtttatc agtttatcaa
      121 atttattgtt acttactgct tgtgggactt ctgcatcaaa gaacatacct tcagatacgc
      181 aaatttcgaa tagtatcgat tcaacaattt ctacaaatga gaaaaattct ccggtagaaa
      241 cttccgatat aaaagtttct acctctgcaa aaaacagggt aaagaaactg tcagcacgtc
      301 tcctttattt caaacaaact ctgaattagc ttcttcatct tcttcagcaa ataatgatga
      361 tagtttcgcg tttactccta actaaatgca acctttacaa atgctttacg ataacaagcc
      421 tgagctaaat gatcctgata tcagttttgc attttttgaa atgattggat cggatgatct
      481 ttttaaaact actagtttgt ctatccgaaa ataggacagt tctggca
//
LOCUS       DACDSR010000288          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDSR010000288 DACDSR010000000
VERSION     DACDSR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.301"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK15_003005"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK15_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9548499.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDSR010000289          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDSR010000289 DACDSR010000000
VERSION     DACDSR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.302"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            57..>517
                     /locus_tag="IWK15_003006"
     CDS             57..>517
                     /locus_tag="IWK15_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9548500.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DACDSR010000290          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDSR010000290 DACDSR010000000
VERSION     DACDSR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.303"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDSR010000291          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDSR010000291 DACDSR010000000
VERSION     DACDSR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.304"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWK15_003007"
     CDS             99..293
                     /locus_tag="IWK15_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548501.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          213 a           65 c           84 g          143 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atata
//
LOCUS       DACDSR010000292          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDSR010000292 DACDSR010000000
VERSION     DACDSR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.305"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            301..>487
                     /locus_tag="IWK15_003008"
     CDS             301..>487
                     /locus_tag="IWK15_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9548502.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVF"
BASE COUNT          189 a           59 c           72 g          167 t
ORIGIN      
        1 atagtaaatt acctcatatg tgagcctgca tatactacga ctattttgac aacagtatta
       61 gaaaatatta acgaagtttc aattttagaa gtaaagaagc tagtgattca tttgctaaat
      121 tcaacagaag atgaatcact aatcccctat ttaaaagatt cacatttttt taaagaagcg
      181 gtactatcta aagaatatag agagcatgat gttttgagat acgcttacat ttattaaata
      241 aaatttctga tagtatttta ataattcgga tcatatcata agatatagga gggaaaagaa
      301 gtgattttat tgattttgtc tctcgtatta ctagttgtat ctaatctaat gaaaaataat
      361 cctcctacaa aaattaattc tttttatggc tttagaacta aaaagtcaat gaaaaatgaa
      421 aaaagttggc atttagcaca aaaattgttg ggggattttt caaaaaaaaa agttatccca
      481 gtatttt
//
LOCUS       DACDSR010000293          474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDSR010000293 DACDSR010000000
VERSION     DACDSR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.306"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IWK15_003009"
     CDS             complement(61..258)
                     /locus_tag="IWK15_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9548503.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          154 a           88 c           66 g          166 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttcc
//
LOCUS       DACDSR010000294          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDSR010000294 DACDSR010000000
VERSION     DACDSR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.307"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>473
                     /locus_tag="IWK15_003010"
     CDS             <1..>473
                     /locus_tag="IWK15_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548504.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           71 c          112 g           98 t
ORIGIN      
        1 accgcaggag aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc
       61 gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
      121 aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
      181 gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg
      241 actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta
      301 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa
      361 aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt
      421 acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DACDSR010000295          465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDSR010000295 DACDSR010000000
VERSION     DACDSR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.308"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..188)
                     /locus_tag="IWK15_003011"
     CDS             complement(<1..188)
                     /locus_tag="IWK15_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548505.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEE"
BASE COUNT          151 a           79 c           65 g          170 t
ORIGIN      
        1 tcctcttcag aacttatgga ttgtccttct tttattttat tgttttcatt acttttttga
       61 cacccaactg cgcttaagct caaacataat gcaagtaata ggcatgctcc tcttggtact
      121 attttttcat aaattattcc tcctttattt tttacacaat agcattttat agctactttc
      181 aataacattc tacacacctt ttatgaaacg ttgatgaaat ataatttttt atttttccaa
      241 actatggtat aacaatataa gtattatgct taaagttgtt cttacacgat tagtatatga
      301 tcgattaata tatgagcaag cacagtaagt gtacggacac ttacgaaaaa attgatgaag
      361 cagccctttg ggatctgctt catcaatttg aatcggctac actagactag acagaaaaaa
      421 taaggtgtgt agaataataa aaaacacact ggaggatttt tcatg
//
LOCUS       DACDSR010000296          456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDSR010000296 DACDSR010000000
VERSION     DACDSR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.310"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(225..371)
                     /locus_tag="IWK15_003012"
     CDS             complement(225..371)
                     /locus_tag="IWK15_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9548506.1"
                     /translation="MEEKNRLNAKQCSEQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT          165 a           72 c           54 g          165 t
ORIGIN      
        1 attataggta tagctgcgga aacatcagta aataattcaa caaaaaaagt gagtggtatc
       61 aataaacata caaaaagata ttttcttttg ttaagatcag tattagtcat ataaaataag
      121 agaaaaaaga aaaagtttcc cacgaacatt cttaatagat aagcttttgt atcgatagta
      181 atcaagagat cccctcctga ttttaggata tcataaaaaa aattttagtt acacttttta
      241 aataatgaaa aaacacattc cactaaattt gatgccggtt ttccttgagg ttttgttcca
      301 gcacctcctt taattttttt aagctcttgt tctgaacatt gtttagcatt tagtctattt
      361 ttttcttcca ttacgattcc ccctacaaac tataaatact tttatgtcac tgattcaata
      421 tacctatttt tctaatcaaa aaattttgag acatat
//
LOCUS       DACDSR010000297          450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDSR010000297 DACDSR010000000
VERSION     DACDSR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.311"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..>450
                     /locus_tag="IWK15_003013"
     CDS             102..>450
                     /locus_tag="IWK15_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9548507.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          181 a           53 c           92 g          124 t
ORIGIN      
        1 atttaagcac aaatagcgga gctgaaagta atcttctgga tgaattaaaa agaactgaga
       61 ttcaaattat agataaaaaa gaacgtccta aaaatggttt tgtgctatca gaaaaaactg
      121 atttagaaaa tacagaagca tttaacaaag aatcggttaa agatttacgt aaactaccaa
      181 caaaggacat aaatggcaag gattttacaa gtaaagattt tgagaagtat gatcttacta
      241 tggtaaatgt atttgcaact tggtgtacag cttgtgtaaa ggaaattcct gatttggttg
      301 aggttcagaa tgaaatgaaa agcaagggtg taaatatcgt tggagttgtt acagatcctg
      361 ttgatgataa cggtgaaaac aaggaagcaa ttgaaaagtc aaagttaata catgaaaaga
      421 caaaggcttc atatcctttc ttaatgccag
//
LOCUS       DACDSR010000298          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDSR010000298 DACDSR010000000
VERSION     DACDSR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.312"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWK15_003014"
     CDS             complement(<1..339)
                     /locus_tag="IWK15_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548508.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDSR010000299          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDSR010000299 DACDSR010000000
VERSION     DACDSR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.313"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWK15_003015"
     CDS             <1..379
                     /locus_tag="IWK15_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9548509.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDSR010000300          398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDSR010000300 DACDSR010000000
VERSION     DACDSR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.314"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..>398
                     /locus_tag="IWK15_003016"
     CDS             122..>398
                     /locus_tag="IWK15_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9548510.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          129 a           71 c           72 g          126 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DACDSR010000301          395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDSR010000301 DACDSR010000000
VERSION     DACDSR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.315"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..395
                     /locus_tag="IWK15_003017"
                     /pseudo
     CDS             1..395
                     /locus_tag="IWK15_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          168 a           68 c           74 g           85 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaat
//
LOCUS       DACDSR010000302          376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDSR010000302 DACDSR010000000
VERSION     DACDSR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.316"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          150 a           32 c           62 g          132 t
ORIGIN      
        1 aataatagaa attaacaagt tacaaaaaaa tattataaag aataatattt tctaaaaagt
       61 aaactatagt aatgtggatt atcaccttcc aacagtatat aaaatgagcg taaaaacggc
      121 tatttttgcc ttaacaacga tttttttgta tacaaatacg atatgctcat taattattgg
      181 aatttgtagt gctgtttaaa ttaatgaagg tgtaattata tgagtgatca ttttgaaaaa
      241 gtttgttgag aagatgtgct tctagtcatg tgatgggaga aaataattta actgttagag
      301 tgaatagtta aattattctg gttatatgaa aaaagtagtt tctaaaataa tcttgcaggg
      361 attaacttaa agaaat
//
LOCUS       DACDSR010000303          356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDSR010000303 DACDSR010000000
VERSION     DACDSR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.317"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>356
                     /locus_tag="IWK15_003018"
     CDS             <1..>356
                     /locus_tag="IWK15_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9548511.1"
                     /translation="EQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAI
                     DYCRLYVGLVDDANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLEC
                     KCYTILLLLSRIRKII"
BASE COUNT          147 a           34 c           65 g          110 t
ORIGIN      
        1 agaacaagag tctattacaa aaagtttatc tccaaataag cagacggaat ttaaagaatt
       61 tttaataact ctaaaagcta tagcatcgaa agaagattta gaatgtcaaa tcagagcaaa
      121 acgtatggca attgattatt gtaggcttta tgtgggctta gttgatgatg caaatttggg
      181 aattaaagag aaaagaaagt atataaaaaa atttattgaa gatgagggat taattcaatg
      241 ttggaaggga tatcctacaa atacgctacc atttcaacaa aaattttttc ataaaatgct
      301 tgaatgtaag tgttatacaa tattgttgtt gttaagtaga attagaaaga taatta
//
LOCUS       DACDSR010000304          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDSR010000304 DACDSR010000000
VERSION     DACDSR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.318"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..321)
                     /locus_tag="IWK15_003019"
     CDS             complement(<1..321)
                     /locus_tag="IWK15_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9548512.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           95 a           55 c           62 g          129 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg t
//
LOCUS       DACDSR010000305          323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDSR010000305 DACDSR010000000
VERSION     DACDSR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.319"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWK15_003020"
     CDS             complement(<1..302)
                     /locus_tag="IWK15_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9548513.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DACDSR010000306          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.denovo.320, whole genome shotgun sequence.
ACCESSION   DACDSR010000306 DACDSR010000000
VERSION     DACDSR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.denovo.320"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWK15_003021"
     CDS             complement(<1..>321)
                     /locus_tag="IWK15_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9548514.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACDSR010000307        10008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.093, whole genome shotgun sequence.
ACCESSION   DACDSR010000307 DACDSR010000000
VERSION     DACDSR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.093"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..720
                     /locus_tag="IWK15_003022"
     CDS             <1..720
                     /locus_tag="IWK15_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548515.1"
                     /translation="GRQVFRRPRQMMNRQTRRFQRSMSKIFRGKAGSTKQKTMTTKQN
                     QTNKANHTRQNERTTPKKETALSNVKKQQGGIGQRLGEKTAHVLDTKNRLVNKRKQAK
                     QQIQATPTHVKYALHQGKENVTHNVTDFKQSMADTKAQRKQQRMNQQEQKRKNIAQKR
                     LELDKAKEKRRKENPSSPVQNNQQHVRKRQQTVTPVLSQNTKRENHQRPLKKEQKQKT
                     PPKQFNQRKLNKRTDKRGQQT"
     gene            717..1739
                     /locus_tag="IWK15_003023"
     CDS             717..1739
                     /locus_tag="IWK15_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9548516.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            1756..2685
                     /locus_tag="IWK15_003024"
     CDS             1756..2685
                     /locus_tag="IWK15_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9548517.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            2930..3046
                     /locus_tag="IWK15_003025"
     CDS             2930..3046
                     /locus_tag="IWK15_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9548518.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3062..3865
                     /locus_tag="IWK15_003026"
     CDS             3062..3865
                     /locus_tag="IWK15_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9548519.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            4040..4336
                     /locus_tag="IWK15_003027"
     CDS             4040..4336
                     /locus_tag="IWK15_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548520.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4343..6295
                     /locus_tag="IWK15_003028"
     CDS             4343..6295
                     /locus_tag="IWK15_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9548521.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(6367..6864)
                     /gene="dfrG"
                     /locus_tag="IWK15_003029"
     CDS             complement(6367..6864)
                     /gene="dfrG"
                     /locus_tag="IWK15_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9548522.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            7226..8266
                     /locus_tag="IWK15_003030"
     CDS             7226..8266
                     /locus_tag="IWK15_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9548523.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            8364..8552
                     /locus_tag="IWK15_003031"
     CDS             8364..8552
                     /locus_tag="IWK15_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9548524.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(8610..8963)
                     /locus_tag="IWK15_003032"
     CDS             complement(8610..8963)
                     /locus_tag="IWK15_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9548525.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            9494..9916
                     /locus_tag="IWK15_003033"
     CDS             9494..9916
                     /locus_tag="IWK15_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9548526.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            9913..>10008
                     /locus_tag="IWK15_003034"
     CDS             9913..>10008
                     /locus_tag="IWK15_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9548527.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYR"
BASE COUNT         3501 a         1670 c         1859 g         2978 t
ORIGIN      
        1 ggcagacaag tgtttcgcag acctcgtcaa atgatgaatc gacaaaccag aagatttcaa
       61 cgcagtatga gtaagatttt tcgtggaaaa gcagggtcaa cgaagcaaaa gacaatgaca
      121 acaaagcaga atcaaaccaa taaagctaac catactcgtc aaaatgaacg gactacacca
      181 aagaaagaaa cagcactttc caatgtgaaa aagcaacaag gcggtatcgg gcaacgatta
      241 ggtgaaaaga cagcccatgt attggacacg aaaaatcgtc tagtcaataa aagaaagcag
      301 gcaaaacaac agatacaggc gacaccaacc catgtcaaat atgcccttca tcaaggaaaa
      361 gagaatgtca ctcataatgt aacagacttt aaacaatcta tggcagatac caaagctcaa
      421 cgcaaacaac agcgtatgaa ccagcaggag caaaaacgaa agaacattgc tcaaaagcgt
      481 ttggagctgg ataaagcaaa agaaaaaaga cgaaaagaaa acccatcatc tcctgttcag
      541 aataaccagc agcatgttcg caagcgtcag caaaccgtca cgcctgtttt gtcacaaaac
      601 actaaacgtg aaaatcatca gcgaccattg aaaaaagagc aaaaacaaaa gacaccaccg
      661 aagcaattca atcagaggaa gctaaataaa agaacggata agcgaggaca acaaacatga
      721 agctaaaaat aacagtgatt tgtagtgtgg tcttctctct cttctttttt ctcttattat
      781 tcctagtcat tttctttgca gatgatacgg acagtggcga aaacaacaag gattcttcta
      841 ttccacaagg cggtgtgacc gtttcaccag aagtgttggc acaccgtccg ttgattgaaa
      901 aatatggtaa ggaatatggg attgaagact atgtttctta tatcttagcc attatgcaag
      961 tggaatcggg cggtacagca gaagatgtta tgcaaagttc cgaatctttg ggtttaccgc
     1021 ccaacagttt gagtaccgaa gagtccatca aacaaggggt taaatatttc agtgaattat
     1081 taaccagtgc agaacaacaa ggagtggata tagattctgt tatccaatct tataattatg
     1141 gcggtggctt tttaaattat gtgaggagtc atgggaaaaa atatacctat gaattagccg
     1201 aacagttttc taaagagaag tcgggtggtc aaaaagcaga ctatcccaat cctatagcca
     1261 tacctgtgaa tggtggttgg cgatataact atggaaatca gttttatgtt cagttggttt
     1321 cacaatacct aaccgacact tccccaacag aatttgatga tgaaaccgtg caagtcatta
     1381 tggacgaagc cttgaaatac gaagggtttc cctatgtctt tggaggggcg tcgcctacca
     1441 cctcttttga ttgtagtggc ttaatacaat gggtgtatga caaagctggc atttctctcc
     1501 ctcgtgtcgc ccaagatcag tatgacgcta cgcaagaaat ctctatggaa gaagcccaag
     1561 caggcgacct tatcttcttt cattcaacct ataatgcagg cacatatgtg actcatgtgg
     1621 cgatatattt agagggaaat cgcttttatc atgcaggcga cccaatcggt tatggcgact
     1681 taagcagtcg ttattggcaa gaccatctga ttggggctag acgtgttatt cataactaag
     1741 aaaggaatga atcaaatgat tataaaaatc gagcgaaaag aaaagaagga gaaaccaaag
     1801 aaatctaaaa aaccgagcaa gcaaagaaaa gttccgatga ttaaagttgg cacgcataag
     1861 aaattaacct ttgttttatg ggttttattg attggtagtg tcggatttgg aatctataaa
     1921 aattttactg ccattgacac tcatactgtc cgtgaaacag aaatcattaa acagcagatt
     1981 gtcgatacca accaagtaga aagttttgtg aaatcctttg cgaaagacta cttttcgtgg
     2041 caacaatccc aagaagccat tgataaacga aatgaaaaat tgacccatta tttgacggaa
     2101 gaattgcagg tattaaatga agaaatgatt cgtaaggata ttcctactag ttctagtgtg
     2161 aacgatatac aggtgtggca ggtttctcaa gtgaatgaga acacctttga ggtactgttt
     2221 tctgttgaac aagttattac cgaagacaaa gacaaggaaa ctatatcgtc tagctttcat
     2281 gtggttgtcc atatagatga atcagataat atggttatta tcaaaaatcc gacaatgagt
     2341 aagaaacctc aaaaatcgga ctaccaaccc aaacagctag aaagtgacca tacagtagat
     2401 acagaaacga tggacgaaat cactagcttt ttagaaacgt tcttccagct ctatcctacc
     2461 gccacagaaa aagaattgac ttactatgta agtaatcatg tgttacctat gattaacaag
     2521 gaatatgttt tcgaggaatt ggtgaatcct atctttacca gaaaagataa ccaagtaatc
     2581 gtgaatgtcg ctgtgaaata tttagatcaa gaaacaaagg caacccaaat ctcgcaattt
     2641 gagttgatat tagaaaaaca agataattgg aagattgtaa aataacaaat attggtacat
     2701 gattacagat actttgtaat catgtactct ttttgataaa aaattggaga ttcctttaca
     2761 aatatgctct tacgtgctat tatttaagtg actatttaaa aggagttaat aaatatgcgg
     2821 caaggtattc ttaaataaac tgtcaatttg atagcgggaa caaataatta gatgtccttt
     2881 tttaggaggg cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa
     2941 gtatccagag aatatctgta tgctttgtat acctatggtt atgcataaaa atcccagtga
     3001 taaaagtatt tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaaaatca
     3061 catgaaaatt attaatattg gagttttagc tcatgttgat gcaggaaaaa ctaccttaac
     3121 agaaagctta ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac
     3181 aacgaggacg gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat
     3241 aacctctttt cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga
     3301 tttcttagca gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc
     3361 tgcaaaagat ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg
     3421 gattcccaca atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt
     3481 ttatcaggat attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact
     3541 gtatcctaat atgtgtgtga cgaactttac cgaatctgaa caatgggata cggtaataga
     3601 gggaaacgat gaccttttag agaaatatat gtccggtaaa tcattagaag cattggaact
     3661 cgaacaagag gaaagcataa gatttcagaa ttgttctctg ttccctcttt atcatggaag
     3721 tgcaaaaagt aatataggga ttgataacct tatagaagtt attactaata aattttattc
     3781 atcaacacat cgaggtccgt ctgaactttg cggaaatgtt ttcaaaattg aatatacaaa
     3841 aaaaagacaa cgtcttgcat attaagtagt tcagttttgg agtacaaaaa gtgaaaatgt
     3901 aaacctaaat gagtatcaaa ttgcaactga atttgatact cgttttttat tgttaaatca
     3961 acgtttgttg ttaatttaca ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg
     4021 tgaaataatt attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat ttactaagca
     4081 aaaaggggtg cattttccta tgcaatttct tgagccaaaa aataagaacg ctaagtctgt
     4141 ggattgggaa atatcagagc aagtaagggt gatagttaag caatacgctg aatatgcaga
     4201 gcgtactgaa agcgaagcag tggatgaatt cttattgaac attttagacg ataagaagtt
     4261 tattgaatgg attgccaaca agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa
     4321 agatagagtg ggttgataga caatgaccaa gcttaaaaga ttagccacga aggaagatga
     4381 aattgtagat gttaaaattc cgatttcaaa cgaagaatta aaagataggg ctaaacaata
     4441 tgatttatta actccaaaac gatttgcaac aaggtataac aaaatgttgt tcttgccaac
     4501 gtcttttaaa tggaacggtt ctgaatatcc cattcaatat aattactgca tcaatccgtt
     4561 ttgttgtaat tttgggaaag aacagcacaa attcaaagat gtaaaaggga aaccaagtcg
     4621 atataaaatg actggtagtt ccaaagataa aggtcataaa ggtatgtact gcaatgacaa
     4681 tcctattggc agaggtgtat cgcaaaactg tacagtaacc cctttatcta attggtctgt
     4741 tgttgaagaa attaaacgcc ttatagaaat caatagcatc caagatgttg agccagatta
     4801 tcaatttcat aaagaaggct gctcggaaga agaatcaacc ccattcaatg agccgaagca
     4861 attttacaaa agggggaaaa gtaggggcaa atctcaacgc tatcaatgta aggcgtgtaa
     4921 gaagttcact aatgttctac caaaaagaga agaaaccact acttatcacc aacaaaagaa
     4981 caccattctt ccaatgttcg ctaagatggt tgttggaagg gtttcagtca gtcgtacttg
     5041 tgatatttta ggaattggag ttggaaccta ctatcataag ctggagtggc tgtataggcg
     5101 ttgtttggag tttttagaac gatatgagac acaacccctt caaacaaaga aatttaacga
     5161 gatgtggctt aatacggaca agatgcacta ctacctaaat aacgtccgta agaaaggaca
     5221 aggttcaaag aaatatacgg ggtttgaaga ccttaatatg caaacgtata tagttgtatc
     5281 tgctgaggtc ttatcaagat atgtgttccg ttctgatgtt gcctatgatt ggaatatttc
     5341 aatggatgaa ttaaatgaag atacaagaaa attcaaagaa gaccatctca atacttttag
     5401 tcgtaaaaat gataggttag attggtctta ttatccacaa gaaccatccg ctaacgatag
     5461 cgaaaatagg aatgcttatc ttcacgaatt agggaaaatt actaatagga gtagatttgt
     5521 cgatggttta aatgttgatg ctccttatac tacaactgct cactattggt taattaaaca
     5581 aatggttaat gccgacgaat ggcgaatgat tagtgatgat gacttctcta ttagaaacgc
     5641 cttttataga gtattcacga aagaattaag gttgagcgat gcacatcatt ttatttgcca
     5701 agttaacaag acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag caaaagcaga
     5761 actgctcgat tggggagata tcagaggatt caaaacaaag tttctacgga ctttggcatc
     5821 tcattatcta actgaattac taacaagtca tcaattccac gaagaggcta ttagtaaaga
     5881 tggagaaaga tatcgcaaat atgccgataa ccctatcaaa catccattag ccactaagga
     5941 taaaggattt tattcagttg attgtagaac ggatttatct gctctggaac caaacgaaat
     6001 cgcaaagatg ctattaaatg ttaatgacca ttcgaccaat agctttattc aacagataag
     6061 acgttatata tcatctcttg aaagaccatt aacaacagct cgtggtgata agaagagcta
     6121 tatttacgct aacttcaatc cgaagtatgc tcaatttgcg attacaattc taagaactta
     6181 ctacaacttt tgtcgccctt ttaaatcagc ggataaaaag gttttgacac ctgctcaaag
     6241 attagggatt acagataaac aatttgattg gaaagatata atttatttca aatgacaaaa
     6301 agagcttgga gtaatcccaa gctcttgtgc tttatcttta ctgttcttct tcaattaacg
     6361 cacccgttaa ctcaataagt tttttctttc atatacatga aaatagtagt tatacggatt
     6421 tttatcattc tttatccctt tttgggcaaa tacctcattc cattcctcat aattcacttc
     6481 tggaaaaaaa gtatctcctt cgaattcatg atgtattttt gtgatgtaca ttttctcaac
     6541 ataagggaaa aacaaattat aaatctgttc tcctccgaaa ataaaaattt cttcttcgtt
     6601 tttacataac tcaaaaacat cttctattga atgaacaatt tcacaaccat taaaggtaaa
     6661 ccccttatct ctcgtcagaa taatatttct tctgtcaggt aaggctcttc cgattgattc
     6721 aaggttcttc ctacctaata ttatcggatg tccctttgta gtatttttaa catattccca
     6781 gtccttggga atcctccaag gaatgtcatt ctctttgcca atcactctat tcttatccat
     6841 cgcagcaatc aaagaaactt tcatttataa cacagccttt ttaactaatt taccatctaa
     6901 tttcttattt ctatcaaaaa accttttctc attatcctgc ccgttagttg aataagcaat
     6961 tttttctata agttatatta tcataatacc acaaaatcac aaattttcat tcaattatca
     7021 ttttcaattg cattttataa atcattttgt atgtaatttg gtatgtaagt taaaatgaaa
     7081 aaagtagtcc attttgaagt acattttgtc attttgtact ccaaaactga actacttact
     7141 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
     7201 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
     7261 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
     7321 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
     7381 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
     7441 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
     7501 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
     7561 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
     7621 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
     7681 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
     7741 tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg
     7801 cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta agtacggttt
     7861 atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta ttgtactgga
     7921 gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaagttta
     7981 tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt gtgcaaatat
     8041 cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc ctgctcgatg
     8101 tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg tttgtttagc
     8161 agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc gtcgtctaaa
     8221 tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg ttttatgttg
     8281 ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata gatttgaatt
     8341 aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt cctatttgtg
     8401 gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc cctttatact
     8461 gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata ataacaatca
     8521 aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct catcttatcg
     8581 gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc ttcattgata
     8641 ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc catgtatttt
     8701 tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa aaattcatca
     8761 atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg gtgttgtcct
     8821 ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc cacttgttca
     8881 cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc tctaaaatcg
     8941 tatttatctt cttttttacg catactaaat cacctctata cattttactg ttcctatata
     9001 accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata gaacactgtg
     9061 gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt tatttaccta
     9121 tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc gacttaatgg
     9181 agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt aagttcttat
     9241 caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt ttgcagatta
     9301 catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg tgcttttttg
     9361 gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc ccccgttcgt
     9421 gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc acgacagaaa
     9481 gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc agtttgacta
     9541 tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc tagacagact
     9601 atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact ctttttctac
     9661 tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa gaatcaatgt
     9721 tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac ggaacattat
     9781 tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac taaatttgaa
     9841 tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag aattcatgaa
     9901 ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat tgtaaaggcg
     9961 acagatggcg acgtggaagc aattaatcag attgttaaac attatcga
//
LOCUS       DACDSR010000308         5985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.125, whole genome shotgun sequence.
ACCESSION   DACDSR010000308 DACDSR010000000
VERSION     DACDSR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.125"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IWK15_003035"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IWK15_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IWK15_003036"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IWK15_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9548528.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IWK15_003037"
     CDS             1851..3005
                     /locus_tag="IWK15_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9548529.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="IWK15_003038"
     CDS             3220..4188
                     /locus_tag="IWK15_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9548530.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="IWK15_003039"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="IWK15_003039"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9548531.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="IWK15_003040"
     CDS             5218..5826
                     /locus_tag="IWK15_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9548532.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1347 g         1666 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DACDSR010000309         5149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.134, whole genome shotgun sequence.
ACCESSION   DACDSR010000309 DACDSR010000000
VERSION     DACDSR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.134"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..163
                     /locus_tag="IWK15_003041"
     CDS             <1..163
                     /locus_tag="IWK15_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9548533.1"
                     /translation="ELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARW
                     EYLSEWVED"
     gene            175..1122
                     /locus_tag="IWK15_003042"
     CDS             175..1122
                     /locus_tag="IWK15_003042"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9548534.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1142..1669
                     /locus_tag="IWK15_003043"
     CDS             1142..1669
                     /locus_tag="IWK15_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9548535.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1732..2385)
                     /locus_tag="IWK15_003044"
     CDS             complement(1732..2385)
                     /locus_tag="IWK15_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9548536.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2517..3359
                     /locus_tag="IWK15_003045"
     CDS             2517..3359
                     /locus_tag="IWK15_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9548537.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3517..4413
                     /locus_tag="IWK15_003046"
     CDS             3517..4413
                     /locus_tag="IWK15_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9548538.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            4416..5129
                     /locus_tag="IWK15_003047"
     CDS             4416..5129
                     /locus_tag="IWK15_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9548539.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
BASE COUNT         1873 a          906 c         1024 g         1346 t
ORIGIN      
        1 agaacttgaa gaaaaaatct cattgctcca agaagagatg aatcatcaag gagataattt
       61 cactcgatta caagaattgc aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa
      121 aatggcacgg tgggaatact tgagtgaatg ggtagaagat taggaggaaa aaggatggaa
      181 caagcgtatc ttgacttagg aagaaaattg ttggaagaag ggcatcgtaa agaagacaga
      241 acaggcacag gcacattaag cttattcgga taccaaatgc ggtttgatct gcaaaaaggg
      301 tttcctttgt taacaacgaa acgagtacct tttggattga tcaaaagcga attgctttgg
      361 tttttaaaag gagatacgaa tatccgctat ctattacaaa ataaaaacca tatttgggat
      421 gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga tatgactgat
      481 tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa gcagagcaag
      541 cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg cgatcttgga
      601 cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa
      661 atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt gatcgtttct
      721 gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac gatgtttcaa
      781 ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg ggatgtgttt
      841 ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat tgcacatgaa
      901 acaggtttag cggttggcga atttgttcac acattaggag atgctcatct ttatatcaat
      961 catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc gactttagtt
     1021 ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca attagaagga
     1081 tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa aggatgagaa
     1141 aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag aagaccgtct
     1201 tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag caaatacact
     1261 agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga atcgtcagac
     1321 gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca tgcatagtgt
     1381 ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg ctggagggtc
     1441 agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag tcatccatca
     1501 tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt cactgatcaa
     1561 tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg aaacgtatca
     1621 gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa aaagctatgg
     1681 atagactagc tgttatttag agctgtccca tccatagctt tatttttttt attaagtaaa
     1741 gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat gccaaacaac
     1801 atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt aagaaagccc
     1861 gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag gaacagcagc
     1921 cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg atactctctc
     1981 ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca accagattaa
     2041 tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag gagtataact
     2101 tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct tttttgcttt
     2161 tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc
     2221 atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt ttactaaaag
     2281 aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg cattaaacac
     2341 ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa cggctccttt
     2401 actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata gaaattgtct
     2461 tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag aaaataatga
     2521 caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta cgcgatgctt
     2581 taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat cccgatgatg
     2641 accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa
     2701 caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga gaagacggca
     2761 gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa gcagcaagac
     2821 aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc actgaccaag
     2881 gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa acagtagaga
     2941 aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc ggaatatcca
     3001 cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg ctgtctagtg
     3061 ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct gtaactaagg
     3121 ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa ctgaaagaat
     3181 tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg gcgcaagaat
     3241 tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg catacgaatc
     3301 cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat gtagattagt
     3361 cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt ttctttataa
     3421 aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata aaatttttga
     3481 taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa acgacatttg
     3541 atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt agctgcttct
     3601 ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga tccaaataaa
     3661 aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt aggagatcaa
     3721 accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg ttataatttg
     3781 accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca gatcttaaag
     3841 cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt tatcacactg
     3901 actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg gttaacggtc
     3961 aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact attggaagaa
     4021 tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa tccattttat
     4081 ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa caaaggaaca
     4141 gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga tttgctttat
     4201 caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac aactagcacg
     4261 acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca tttccatcca
     4321 aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat cgagacacaa
     4381 aaccaatggt tagagaagga ggatgatcaa taaacatgaa aacaagatct gacgtatctt
     4441 caaacaaaac aaagaaaaca aattactgga aattcgcctt ccttatcctt cttggggtag
     4501 tacttggttc gaccgtattt ttaggcagcc gtatttttgc taatcgggaa ccagaactcc
     4561 cagaaatacc agctcttacg gaaagacaag gagatcctgt cttaacgatc aattcaaata
     4621 aagaaaaagt caatcaaatc atcagctttt tccttagtga gtatcaaaaa gatagtgata
     4681 ttgaatataa attttatttg gaaaatgaag cactactcaa tggtactttc gaagtactag
     4741 gttttcctat cgatttctat ttatattttg atccgtatgt gatggaaaac ggaaatgtcc
     4801 aactaaaagc tagaagtctt tctatcggaa cattgagttt gccaatcaaa gatgtgatga
     4861 atatgatcaa gagaaactat aaattaccag attggatcga agtcaataca gatgatctga
     4921 ctatcatgct taggttggat caattccgaa tgcaaaacgg gatgtatatt aaagcagata
     4981 aaatcgactt agtaaatgat gacattcggt ttagtctata tttgccaaaa gatacctctg
     5041 atgcttcaaa caactcaaat accaacaaaa ctagcaccag cagtactgaa agcaagacaa
     5101 aagaaaataa aaaatcatcg gatgagtagt attagagggg gaatcataa
//
LOCUS       DACDSR010000310         3561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.154, whole genome shotgun sequence.
ACCESSION   DACDSR010000310 DACDSR010000000
VERSION     DACDSR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.154"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1045
                     /locus_tag="IWK15_003048"
     CDS             <1..1045
                     /locus_tag="IWK15_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9548540.1"
                     /translation="WEDRKGRKINVPIADGGENTAEAIYYALRGTWRTIDGMDLLFRQ
                     RKISYLLTTWQGKNTAVIESAQALGLDLLDKPTDQTIRLASSYALGELIKDALLQEVE
                     QIIVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRNVLVSKVKLIIA
                     ADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWEVDLDDIPGSGA
                     AGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQQTIHGKVPYGV
                     AVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKIL
                     GQTLSRFFTILPK"
     gene            1303..2805
                     /locus_tag="IWK15_003049"
     CDS             1303..2805
                     /locus_tag="IWK15_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9548541.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2872..3399
                     /locus_tag="IWK15_003050"
     CDS             2872..3399
                     /locus_tag="IWK15_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9548542.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1211 a          614 c          787 g          949 t
ORIGIN      
        1 ctgggaagat cgtaaaggaa gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac
       61 tgcagaagca atctattacg cgctcagggg aacatggcgt accattgatg ggatggatct
      121 cttattccgt cagcgaaaaa tcagctattt attgacaact tggcaaggaa aaaacaccgc
      181 agtcatcgaa tctgcacaag cattggggtt agatctactt gataaaccaa cggatcaaac
      241 tatccgactt gcttccagtt atgccttagg agaacttatt aaagatgcac tgcttcaaga
      301 agtagaacaa attattgtca ctttaggagg aagcgggtgt agcgatggtg gtctaggatt
      361 actccaatcc ttgggtgctt ctttagaagg gtacacagaa ggaaatcctt tattatctac
      421 taaaaaactt cattttgatc gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc
      481 tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat ttggaagaca
      541 aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa agattgctca
      601 gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg gagcagcagg
      661 cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt tcgatttagt
      721 cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca ttacaggaga
      781 agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag cagtgcttgc
      841 aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag agctagaagg
      901 actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag gtttagaaga
      961 agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga cacttagcag
     1021 atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct atcgtaattt
     1081 caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt gatgaaagct
     1141 gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg ggattggctt
     1201 ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct ctttagccag
     1261 ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa aatcgaaata
     1321 aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca agcaaatcta
     1381 aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg aaagacaact
     1441 ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca tcagcaagaa
     1501 tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta cgtgttaaat
     1561 gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat gcaaacagat
     1621 cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac aaaagtccta
     1681 ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc aacgaatcat
     1741 ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa acaaggcttc
     1801 atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt tttagcaatc
     1861 gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga acagaaaaaa
     1921 cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta
     1981 aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga taaaacaaag
     2041 aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc agtgggtgct
     2101 gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga gacccagatc
     2161 agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac gatgaatagc
     2221 caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg gtatgaaaat
     2281 ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc gatttcaggt
     2341 cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt caacggcaag
     2401 gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag ccaaaattat
     2461 gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga ctaccaagca
     2521 tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa caagatcgag
     2581 cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc acagccagct
     2641 gaactatata catgggatga accattgaat tatttggatg tcttcaatca agaacaatta
     2701 gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga tcaaaccttc
     2761 ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt gtaatctcaa
     2821 gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca catgctttat
     2881 gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg acgggcagat
     2941 gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa ggtagtagat
     3001 ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag agatacagca
     3061 caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg
     3121 gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa agcacgttta
     3181 gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc acggatcgtt
     3241 cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt ttgccataat
     3301 gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg gttctttaca
     3361 ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg tgttatttaa
     3421 ataaaatatt tcaaggcact gcttttgctt agcagtgcct tttttaggtt atgacaattc
     3481 ttttgataat ataaactcaa cctttagaaa tgaaatttaa ttattttaat aaaagtattg
     3541 aaattttgtt tctttagata t
//
LOCUS       DACDSR010000311         2962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.170, whole genome shotgun sequence.
ACCESSION   DACDSR010000311 DACDSR010000000
VERSION     DACDSR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.170"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..305
                     /locus_tag="IWK15_003051"
     CDS             <1..305
                     /locus_tag="IWK15_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9548543.1"
                     /translation="KLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQK
                     KIKHCEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            289..918
                     /locus_tag="IWK15_003052"
     CDS             289..918
                     /locus_tag="IWK15_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9548544.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1272..2750
                     /gene="msr"
                     /locus_tag="IWK15_003053"
     CDS             1272..2750
                     /gene="msr"
                     /locus_tag="IWK15_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9548545.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2797..>2962
                     /locus_tag="IWK15_003054"
     CDS             2797..>2962
                     /locus_tag="IWK15_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9548546.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDV"
BASE COUNT         1026 a          485 c          634 g          817 t
ORIGIN      
        1 aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg
       61 gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta gcgctaacag
      121 aaaaagccca gaagaaaatc aagcattgtg aaatggacca taaagataca ggttccgtgc
      181 tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta gataaattga
      241 atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat ggaacaaaaa
      301 aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga tgaaacatac
      361 aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc accttcttct
      421 gtcaatatga aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg
      481 cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat ggtcatgatt
      541 tttggcgata tgcaatgtta cgaatatggc gaagaaatct acaaccaagc ttacgaatca
      601 gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc ttattacaag
      661 agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt agcggctatg
      721 caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg tggattcgaa
      781 gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc agttattatt
      841 ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc accagaaaag
      901 atcactatgt tcgaataaaa ggttttcagt tactattcag tagctattgc tttttttgaa
      961 tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg atgaaattac
     1021 gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa caacaatttc
     1081 atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc tatgacagat
     1141 ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt
     1201 gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga
     1261 gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca
     1321 accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca
     1381 taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc
     1441 ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc
     1501 tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag
     1561 aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc
     1621 tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa
     1681 gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc
     1741 atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa
     1801 tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga
     1861 ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc
     1921 aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac
     1981 aaatcagact ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc
     2041 aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc
     2101 agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc
     2161 ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg
     2221 attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga
     2281 caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa
     2341 gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt
     2401 ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag
     2461 ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc
     2521 gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga
     2581 ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg
     2641 gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt
     2701 atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg
     2761 atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga
     2821 ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc gtataattta
     2881 tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc tggattcgtg
     2941 gtggctttca ttttggatgt ct
//
LOCUS       DACDSR010000312         2506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.181, whole genome shotgun sequence.
ACCESSION   DACDSR010000312 DACDSR010000000
VERSION     DACDSR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.181"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1121
                     /locus_tag="IWK15_003055"
     CDS             <1..1121
                     /locus_tag="IWK15_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9548547.1"
                     /translation="VQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLL
                     RLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQEC
                     GMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEE
                     FPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMI
                     NNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNE
                     VDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQL
                     ERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1128..1505
                     /locus_tag="IWK15_003056"
     CDS             1128..1505
                     /locus_tag="IWK15_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9548548.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1659..2441
                     /locus_tag="IWK15_003057"
     CDS             1659..2441
                     /locus_tag="IWK15_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9548549.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          938 a          358 c          509 g          701 t
ORIGIN      
        1 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
       61 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
      121 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
      181 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
      241 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
      301 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
      361 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
      421 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
      481 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
      541 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
      601 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
      661 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
      721 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
      781 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
      841 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
      901 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
      961 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     1021 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     1081 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     1141 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     1201 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     1261 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     1321 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
     1381 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
     1441 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
     1501 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
     1561 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
     1621 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
     1681 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
     1741 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
     1801 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
     1861 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
     1921 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
     1981 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
     2041 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
     2101 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
     2161 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
     2221 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
     2281 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
     2341 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
     2401 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
     2461 caggtgctta cttttaaaac tactgattta ttgataaata ttgaac
//
LOCUS       DACDSR010000313         2072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.189, whole genome shotgun sequence.
ACCESSION   DACDSR010000313 DACDSR010000000
VERSION     DACDSR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.189"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..773)
                     /locus_tag="IWK15_003058"
     CDS             complement(111..773)
                     /locus_tag="IWK15_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9548550.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1137..1224
                     /locus_tag="IWK15_003059"
                     /pseudo
     CDS             1137..1224
                     /locus_tag="IWK15_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            1282..2013
                     /gene="erm(A)"
                     /locus_tag="IWK15_003060"
     CDS             1282..2013
                     /gene="erm(A)"
                     /locus_tag="IWK15_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9548551.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          734 a          366 c          305 g          667 t
ORIGIN      
        1 atagtaacat aagatacagg ctgatttttt attcattgtt atttatacta aagcacccga
       61 tagctctgaa aacaatcaca aattcaactt ttcaaagcca cagctttaag ttattttgtc
      121 cagacaaccc ccattgccca acccatttta tggaattggc atccaggcaa caacttttca
      181 tataaatcgt aataattttg ttcagatagg tacttatctg aagctaaatg ctcaagccat
      241 gatttagatg tgttgtgatt ataaattcta atcgcatttt ttattccaaa tttgaaacaa
      301 tgcggtaaaa actcttggat tgcccctaat ttataaacat aggtaggcgg tgtctcgact
      361 tcagatacat tatcaagaat aactattctt ccttcctcat tcaacagttc cttcatttgc
      421 tgtattacgc tggctatatc atccaaatga tgaaaggttg ttcggcttac aataaaatca
      481 aacttctcat taaaattaag ttgttctgca ttcatattca gatagaccgt atttgttagt
      541 tgacgtttag atttggcaag atcgagcatt tgattagaaa tatcaatccc taccacttca
      601 tcataataac ttgctaattt ctccactaac aaacccgagc cacatccgat atctaatgct
      661 ctgcctttct ttggagacat attagacaca aagaatgaat aatcattcaa aagctcattc
      721 acgaaatcgt aatcttctgc aaccttatca aactgtgatt ctattgtatt caaaaagatc
      781 ccccattcct actttatcga cattctttca ttacttacca ctttagatgt tttttcgttg
      841 gggataaaac ttccctttag acaattttat ccaaagacaa tacaacagtg caactttatt
      901 aaagtcactg tcctttatcg cagcctttac tttttagtaa agacagtggc ttctcttatc
      961 aagtttcaaa acatattatt ttgaagaaaa cgtccatctg aagtgtcaag tgcaaaatta
     1021 catataaagg tttattctaa aatgaaaaga tgatacaatc atattcagtt acataaggag
     1081 gtttcaatta tgtgcaccag tatcgcagta gtagaaatta ctttatctca ttcataatga
     1141 aaaaaatgga aaggagataa aagtatgggt actttttcta tatttgttat taataaagtt
     1201 cgttatcaac caaatcaaaa ttaattggtt ataatgaacg cttaatgtca gttcattata
     1261 accagtaagg agaaggttat aatgaaccag aaaaacccta aagacacgca aaattttatt
     1321 acttctaaaa agcatgtaaa agaaatattg aatcacacga atatcagtaa acaagacaac
     1381 gtaatagaaa tcggatcagg aaaaggacat tttaccaaag agctagtcaa aatgagtcga
     1441 tcagttactg ctatagaaat tgatggaggc ttatgtcaag tgactaaaga agcggtaaac
     1501 ccctctgaga atataaaagt gattcaaacg gatattctaa aattttcctt cccaaaacat
     1561 ataaactata agatatatgg taatattcct tataacatca gtacggatat tgtcaaaaga
     1621 attacctttg aaagtcaggc taaatatagc tatcttatcg ttgagaaggg atttgcgaaa
     1681 agattgcaaa atctgcaacg agctttgggt ttactattaa tggtggagat ggatataaaa
     1741 atgctcaaaa aagtaccacc actatatttt catcctaagc caagtgtaga ctctgtattg
     1801 attgttcttg aacgacatca accattgatt tcaaagaagg actacaaaaa gtatcgatct
     1861 tttgtttata agtgggtaaa ccgtgaatat cgtgttcttt tcactaaaaa ccaattccga
     1921 caggctttga agcatgcaaa tgtcactaat attaataaac tatcgaagga acaatttctt
     1981 tctattttca atagttacaa attgtttcac taaattaaag taataaagcg ttctctaatt
     2041 tcacaagagg acgctttatt cttcccaaaa at
//
LOCUS       DACDSR010000314         1569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.214, whole genome shotgun sequence.
ACCESSION   DACDSR010000314 DACDSR010000000
VERSION     DACDSR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.214"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..204
                     /locus_tag="IWK15_003061"
     CDS             <1..204
                     /locus_tag="IWK15_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9548552.1"
                     /translation="PNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYE
                     KLGGKNDYKDEIVYVYDYEKGDK"
     gene            205..>1569
                     /gene="aph(2'')-I"
                     /locus_tag="IWK15_003062"
     CDS             205..>1569
                     /gene="aph(2'')-I"
                     /locus_tag="IWK15_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9548553.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          653 a          118 c          271 g          527 t
ORIGIN      
        1 cctaactatc aagacaaagg ttatggttca aaattattat cttttattaa ggaatattct
       61 aaagagattg gttgttctga aatgttttta ataactgata aaggtaatcc tagagcttgc
      121 catgtatatg aaaaattagg tggtaaaaat gattataaag atgaaatagt atatgtatat
      181 gattatgaaa aaggtgataa ataaatgaat atagttgaaa atgaaatatg tataagaact
      241 ttaatagatg atgattttcc tttgatgtta aaatggttaa ctgatgaaag agtattagaa
      301 ttttatgatg gtagagataa aaaatataca ttagaatcat taaaaaaaca ttatacagag
      361 ccttgggaag atgaagtttt tagagtaatt attgaatata acaatgttcc tattggatat
      421 ggacaaatat ataaaatgta tgatgagtta tatactgatt atcattatcc aaaaactgat
      481 gagatagtct atggtatgga tcaatttata ggagagccaa attattggag taaaggaatt
      541 ggtacaagat atattaaatt gatttttgaa tttttgaaaa aagaaagaaa tgctaatgca
      601 gttattttag accctcataa aaataatcca agagcaataa gggcatacca aaaatctggt
      661 tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt
      721 tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta
      781 attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat
      841 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
      901 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
      961 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
     1021 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
     1081 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
     1141 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
     1201 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
     1261 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
     1321 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
     1381 gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat
     1441 agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata
     1501 gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact
     1561 attgtttat
//
LOCUS       DACDSR010000315         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.217, whole genome shotgun sequence.
ACCESSION   DACDSR010000315 DACDSR010000000
VERSION     DACDSR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.217"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWK15_003063"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWK15_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWK15_003064"
     CDS             394..477
                     /locus_tag="IWK15_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9548554.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK15_003065"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK15_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9548555.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDSR010000316         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.227, whole genome shotgun sequence.
ACCESSION   DACDSR010000316 DACDSR010000000
VERSION     DACDSR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.227"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IWK15_003066"
     CDS             <1..1311
                     /locus_tag="IWK15_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9548556.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DACDSR010000317         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.233, whole genome shotgun sequence.
ACCESSION   DACDSR010000317 DACDSR010000000
VERSION     DACDSR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.233"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWK15_003067"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWK15_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWK15_003068"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWK15_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9548557.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDSR010000318         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.245, whole genome shotgun sequence.
ACCESSION   DACDSR010000318 DACDSR010000000
VERSION     DACDSR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.245"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="IWK15_003069"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="IWK15_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9548558.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DACDSR010000319          832 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20628_2015
            SAMN12639634-rid9882833.guided.275, whole genome shotgun sequence.
ACCESSION   DACDSR010000319 DACDSR010000000
VERSION     DACDSR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639634
            Sequence Read Archive: SRR10030750
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742055.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742055.1
            Genome Coverage       :: 45x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:28:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,006
            Genes (coding)                    :: 2,926
            CDSs (with protein)               :: 2,926
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 33 of 80
            Pseudo Genes (incomplete)         :: 39 of 80
            Pseudo Genes (internal stop)      :: 19 of 80
            Pseudo Genes (multiple problems)  :: 10 of 80
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639634-rid9882833.guided.275"
                     /isolate="E20628_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            117..665
                     /gene="aac(6')"
                     /locus_tag="IWK15_003070"
     CDS             117..665
                     /gene="aac(6')"
                     /locus_tag="IWK15_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9548559.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          299 a          126 c          188 g          219 t
ORIGIN      
        1 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
       61 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
      121 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
      181 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
      241 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
      301 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
      361 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
      421 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
      481 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
      541 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
      601 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
      661 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
      721 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
      781 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aa
//
